Minnesota/9 June 2009

From 2009.igem.org

(Difference between revisions)
Line 14: Line 14:
! Reverse Kinetic Constant
! Reverse Kinetic Constant
|-
|-
-
| RNAp + lacP + tetO1 + tetO2 ↔ RNAp:lacP||1E+07|| 1
+
|1/2||RNAp + lacP + tetO1 + tetO2 ↔ RNAp:lacP||1E+07|| 1
|-
|-
-
|RNA:lacP -> RNAp:lacP* || .01 ||
+
|3||RNA:lacP -> RNAp:lacP* || .01 ||
|-
|-
-
|RNAp:lacP* -> lacP + tetO1 + tetO2 + RNAp:DNAgfp||30||
+
|4||RNAp:lacP* -> lacP + tetO1 + tetO2 + RNAp:DNAgfp||30||
|-
|-
-
|RNAp:DNAgfp -> RNAp + gfp_mRNA||30||
+
|5||RNAp:DNAgfp -> RNAp + gfp_mRNA||30||
|-
|-
-
|gfp_mRNA + rib -> rib:gfp_mRNA||100000||
+
|6||gfp_mRNA + rib -> rib:gfp_mRNA||100000||
|-
|-
-
|rib:gfp_mRNA -> rib:gfp_mRNA_1 + gfp_mRNA||33||
+
|7||rib:gfp_mRNA -> rib:gfp_mRNA_1 + gfp_mRNA||33||
|-
|-
-
|rib:gfp_mRNA_1 -> rib + gfp||33||
+
|8||rib:gfp_mRNA_1 -> rib + gfp||33||
|-
|-
-
 
+
|9||gfp_mRNA -> Ø||1.16E-03||
-
|gfp_mRNA -> Ø||1.16E-03||
+
|-
|-
-
|gfp -> Ø||3.21E-05||
+
|10||gfp -> Ø||3.21E-05||
|-
|-
-
|tetR2 + aTc ↔ tetR2:aTc||2E+09||4E-04
+
|11\12||tetR2 + aTc ↔ tetR2:aTc||2E+09||4E-04
|-
|-
-
|tetR2:aTc + aTc  ↔ tetR2:aTc2||1E+08||1E-03
+
|13\14||tetR2:aTc + aTc  ↔ tetR2:aTc2||1E+08||1E-03
|-
|-
-
|tetR2 + tetO1 ↔ tetR2:tetO1||1E+08||1E-03
+
|15\16||tetR2 + tetO1 ↔ tetR2:tetO1||1E+08||1E-03
|-
|-
-
|tetR2:aTc + tetO1 ↔ tetR2:tetO1:aTc||1E+08||1
+
|17\18||tetR2:aTc + tetO1 ↔ tetR2:tetO1:aTc||1E+08||1
|-
|-
-
|tetR2:aTc2 + tetO1 ↔ tetR2:tetO1:aTc2||1E+08||1E+05
+
|19\20||tetR2:aTc2 + tetO1 ↔ tetR2:tetO1:aTc2||1E+08||1E+05
|-
|-
-
|tetR2:tetO1 + aTc ↔ tetR2:tetO1:aTc||1E+08||1E-03
+
|21\22||tetR2:tetO1 + aTc ↔ tetR2:tetO1:aTc||1E+08||1E-03
|-
|-
-
|tetR2:tetO1:aTc + aTc ↔ tetR2:tetO1:aTc2||1E+08||1E-03
+
|23\24||tetR2:tetO1:aTc + aTc ↔ tetR2:tetO1:aTc2||1E+08||1E-03
|-
|-
-
|tetR2 + tetO2 ↔ tetR2:tetO2||1E+08||1E-03
+
|25\26||tetR2 + tetO2 ↔ tetR2:tetO2||1E+08||1E-03
|-
|-
-
|tetR2:aTc + tetO2 ↔ tetR2:tetO2:aTc||1E+08||1
+
|27\28||tetR2:aTc + tetO2 ↔ tetR2:tetO2:aTc||1E+08||1
|-
|-
-
|tetR2:aTc2 + tetO2 ↔ tetR2:tetO2:aTc2||1E+08||1E+05
+
|29\30||tetR2:aTc2 + tetO2 ↔ tetR2:tetO2:aTc2||1E+08||1E+05
|-
|-
-
|tetR2:tetO2 + aTc ↔ tetR2:tetO2:aTc||1E+08||1E-03
+
|31\32||tetR2:tetO2 + aTc ↔ tetR2:tetO2:aTc||1E+08||1E-03
|-
|-
-
|tetR2:tetO2:aTc + aTc ↔ tetR2:tetO2:aTc2||1E+08||1E-03
+
|33\34||tetR2:tetO2:aTc + aTc ↔ tetR2:tetO2:aTc2||1E+08||1E-03
|-
|-
-
|tetR2 -> Ø||2.89E-04||
+
|35||tetR2 -> Ø||2.89E-04||
|-
|-
-
|tetR2:aTc -> aTc||2.89E-04||
+
|36||tetR2:aTc -> aTc||2.89E-04||
|-
|-
-
|tetR2:aTc2 -> 2 aTc||2.89E-04||
+
|37||tetR2:aTc2 -> 2 aTc||2.89E-04||
|-
|-
-
|tetR2 + nsDNA ↔  tetR2:nsDNA||1000||3.2409
+
|38\39||tetR2 + nsDNA ↔  tetR2:nsDNA||1000||3.2409
|-
|-
-
|tetR2:aTc + nsDNA ↔ tetR2:aTc:nsDNA||1000||3.2409
+
|40\41||tetR2:aTc + nsDNA ↔ tetR2:aTc:nsDNA||1000||3.2409
|-
|-
-
|tetR2:aTc:nsDNA -> aTc + nsDNA||1.93E-04||
+
|42||tetR2:aTc:nsDNA -> aTc + nsDNA||1.93E-04||
|-
|-
-
|tetR2:nsDNA -> nsDNA||1.93E-04||
+
|43||tetR2:nsDNA -> nsDNA||1.93E-04||
|-
|-
-
|Ø -> tetR2||1E-11||
+
|44||Ø -> tetR2||1E-11||
|-
|-
-
|Ø -> aTc||3.3E-04||
+
|45||Ø -> aTc||3.3E-04||
|}
|}

Revision as of 18:57, 29 July 2009

Back to Notebook Home
Go to Previous Day (June 8)Go to Next Day (June 10)

Today Patrick arrived and began working on getting up to speed on the team's TTL model and creating the TTN model he will eventually be working on.
The model created was similar to that described previously except with the addition of a second tet operon (tetO2):

Rxn # Reaction Forward Kinetic Constant Reverse Kinetic Constant
1/2RNAp + lacP + tetO1 + tetO2 ↔ RNAp:lacP1E+07 1
3RNA:lacP -> RNAp:lacP* .01
4RNAp:lacP* -> lacP + tetO1 + tetO2 + RNAp:DNAgfp30
5RNAp:DNAgfp -> RNAp + gfp_mRNA30
6gfp_mRNA + rib -> rib:gfp_mRNA100000
7rib:gfp_mRNA -> rib:gfp_mRNA_1 + gfp_mRNA33
8rib:gfp_mRNA_1 -> rib + gfp33
9gfp_mRNA -> Ø1.16E-03
10gfp -> Ø3.21E-05
11\12tetR2 + aTc ↔ tetR2:aTc2E+094E-04
13\14tetR2:aTc + aTc ↔ tetR2:aTc21E+081E-03
15\16tetR2 + tetO1 ↔ tetR2:tetO11E+081E-03
17\18tetR2:aTc + tetO1 ↔ tetR2:tetO1:aTc1E+081
19\20tetR2:aTc2 + tetO1 ↔ tetR2:tetO1:aTc21E+081E+05
21\22tetR2:tetO1 + aTc ↔ tetR2:tetO1:aTc1E+081E-03
23\24tetR2:tetO1:aTc + aTc ↔ tetR2:tetO1:aTc21E+081E-03
25\26tetR2 + tetO2 ↔ tetR2:tetO21E+081E-03
27\28tetR2:aTc + tetO2 ↔ tetR2:tetO2:aTc1E+081
29\30tetR2:aTc2 + tetO2 ↔ tetR2:tetO2:aTc21E+081E+05
31\32tetR2:tetO2 + aTc ↔ tetR2:tetO2:aTc1E+081E-03
33\34tetR2:tetO2:aTc + aTc ↔ tetR2:tetO2:aTc21E+081E-03
35tetR2 -> Ø2.89E-04
36tetR2:aTc -> aTc2.89E-04
37tetR2:aTc2 -> 2 aTc2.89E-04
38\39tetR2 + nsDNA ↔ tetR2:nsDNA10003.2409
40\41tetR2:aTc + nsDNA ↔ tetR2:aTc:nsDNA10003.2409
42tetR2:aTc:nsDNA -> aTc + nsDNA1.93E-04
43tetR2:nsDNA -> nsDNA1.93E-04
44Ø -> tetR21E-11
45Ø -> aTc3.3E-04