Parts

From 2009.igem.org

(Difference between revisions)
(Parts)
Line 6: Line 6:
'''PDC'''
'''PDC'''
*Primary nucleaic acid sequence
*Primary nucleaic acid sequence
-
ATGAGCGAAATCACCCTGGGCAAATACCTGTTTGAACGCCTGAAACAGGTGAACGTGAACACCGTGTTTGGCCTGCCTGGCGATTTTAATCTGAGCCTGCTGGATAAAATCTACGAAGTGGAAGGCATGCGCTGGGCTGGCAACGCCAACGAACTGAACGCTGCCTACGCTGCCGATGGCTACGCTCGCATCAAAGGCATGAGCTGTATCATCACGACCTTTGGCGTGGGCGAACTGAGCGCTCTGAACGGCATTGCTGGCAGCTACGCCGAACACGTGGGCGTGCTGCATGTGGTGGGCGTGCCCAGCATTAGCGCTCAGGCCAAACAGCTGCTGCTGCACCATACCCTGGGCAACGGCGATTTTACCGTGTTTCACCGCATGAGCGCCAACATCAGCGAAACCACCGCCATGATCACCGATATCGCCACCGCTCCCGCTGAAATCGATCGCTGCATCCGCACCACCTACGTGACCCAACGCCCTGTGTATCTGGGCCTCCCTGCCAACCTGGTGGATCTGAACGTGCCTGCCAAACTGCTGCAGACCCCCATTGATATGAGCCTGAAACCCAACGATGCCGAAAGCGAAAAAGAAGTGATCGATACCATCCTGGCCCTGGTGAAAGATGCCAAAAACCCCGTGATCCTGGCCGATGCCTGCTGCAGTCGCCACGATGTGAAAGCCGAAACCAAAAAACTGATCGATCTGACCCAGTTTCCCGCTTTTGTGACCCCCATGGGCAAAGGCAGCATCGATGAACAGCACCCTCGCTACGGTGGCGTGTATGTGGGCACCCTGAGCAAACCCGAAGTGAAAGAAGCCGTGGAAAGCGCTGATCTGATCCTGAGCGTGGGTGCCCTGCTGTCGGATTTTAACACCGGCAGCTTTAGCTACAGCTACAAGACCAAAAACATCGTGGAATTTCACAGCGATCACATGAAAATCCGCAACGCCACCTTTCCTGGCGTGCAGATGAAATTTGTGCTGCAGAAACTGCTGACCACCATTGCCGATGCCGCTAAAGGCTACAAACCCGTGGCTGTTCCCGCTCGCACCCCTGCTAATGCTGCTGTGCCTGCCAGCACCCCTCTGAAACAAGAATGGATGTGGAACCAGCTGGGCAACTTTCTGCAAGAGGGTGATGTGGTGATCGCCGAAACCGGCACCAGCGCCTTTGGCATCAACCAGACCACCTTTCCCAACAACACCTACGGCATCAGCCAGGTGCTGTGGGGCAGCATCGGCTTTACGACCGGTGCTACCCTGGGTGCTGCGTTTGCCGCTGAAGAAATCGATCCCAAAAAACGCGTGATTCTGTTTATCGGTGATGGCAGCCTGCAGCTGACCGTGCAAGAAATCAGCACCATGATCCGCTGGGGTCTGAAACCCTACCTGTTTGTGCTGAATAACGATGGCTACACCATCGAAAAACTGATCCACGGTCCCAAAGCCCAGTACAACGAAATCCAGGGCTGGGATCACCTGAGTCTGCTGCCGACCTTTGGCGCTAAAGATTACGAAACCCATCGCGTGGCTACCACGGGTGAATGGGATAAACTGACCCAGGATAAGAGCTTTAACGATAACAGCAAGATCCGCATGATCGAAATCATGCTGCCCGTGTTTGATGCTCCCCAGAACCTGGTGGAACAGGCCAAACTGACCGCTGCCACCAACGCCAAACAGTAA
+
#ATGAGCGAAATCACCCTGGGCAAATACCTGTTTGAACGCCTGAAACAGGTGAACGTGAACACCGTGTTTGGCCTGCCTGGCGATTTTAATCTGAGCCTGCTGGATAAAATCT#ACGAAGTGGAAGGCATGCGCTGGGCTGGCAACGCCAACGAACTGAACGCTGCCTACGCTGCCGATGGCTACGCTCGCATCAAAGGCATGAGCTGTATCATCACGACCTTTGG#CGTGGGCGAACTGAGCGCTCTGAACGGCATTGCTGGCAGCTACGCCGAACACGTGGGCGTGCTGCATGTGGTGGGCGTGCCCAGCATTAGCGCTCAGGCCAAACAGCTGCTG#CTGCACCATACCCTGGGCAACGGCGATTTTACCGTGTTTCACCGCATGAGCGCCAACATCAGCGAAACCACCGCCATGATCACCGATATCGCCACCGCTCCCGCTGAAATCG#ATCGCTGCATCCGCACCACCTACGTGACCCAACGCCCTGTGTATCTGGGCCTCCCTGCCAACCTGGTGGATCTGAACGTGCCTGCCAAACTGCTGCAGACCCCCATTGATAT#GAGCCTGAAACCCAACGATGCCGAAAGCGAAAAAGAAGTGATCGATACCATCCTGGCCCTGGTGAAAGATGCCAAAAACCCCGTGATCCTGGCCGATGCCTGCTGCAGTCGC#CACGATGTGAAAGCCGAAACCAAAAAACTGATCGATCTGACCCAGTTTCCCGCTTTTGTGACCCCCATGGGCAAAGGCAGCATCGATGAACAGCACCCTCGCTACGGTGGCG#TGTATGTGGGCACCCTGAGCAAACCCGAAGTGAAAGAAGCCGTGGAAAGCGCTGATCTGATCCTGAGCGTGGGTGCCCTGCTGTCGGATTTTAACACCGGCAGCTTTAGCTA#CAGCTACAAGACCAAAAACATCGTGGAATTTCACAGCGATCACATGAAAATCCGCAACGCCACCTTTCCTGGCGTGCAGATGAAATTTGTGCTGCAGAAACTGCTGACCACC#ATTGCCGATGCCGCTAAAGGCTACAAACCCGTGGCTGTTCCCGCTCGCACCCCTGCTAATGCTGCTGTGCCTGCCAGCACCCCTCTGAAACAAGAATGGATGTGGAACCAGC#TGGGCAACTTTCTGCAAGAGGGTGATGTGGTGATCGCCGAAACCGGCACCAGCGCCTTTGGCATCAACCAGACCACCTTTCCCAACAACACCTACGGCATCAGCCAGGTGCT#GTGGGGCAGCATCGGCTTTACGACCGGTGCTACCCTGGGTGCTGCGTTTGCCGCTGAAGAAATCGATCCCAAAAAACGCGTGATTCTGTTTATCGGTGATGGCAGCCTGCAG#CTGACCGTGCAAGAAATCAGCACCATGATCCGCTGGGGTCTGAAACCCTACCTGTTTGTGCTGAATAACGATGGCTACACCATCGAAAAACTGATCCACGGTCCCAAAGCCC#AGTACAACGAAATCCAGGGCTGGGATCACCTGAGTCTGCTGCCGACCTTTGGCGCTAAAGATTACGAAACCCATCGCGTGGCTACCACGGGTGAATGGGATAAACTGACCCA#GGATAAGAGCTTTAACGATAACAGCAAGATCCGCATGATCGAAATCATGCTGCCCGTGTTTGATGCTCCCCAGAACCTGGTGGAACAGGCCAAACTGACCGCTGCCACCAAC#GCCAAACAGTAA
*Description of function
*Description of function
Line 18: Line 18:
'''ADH'''
'''ADH'''
*Primary nucleaic acid sequence
*Primary nucleaic acid sequence
-
ATGTCGATCCCCGAAACCCAGAAAGGCGTGATCTTTTACGAAAGCCACGGCAAACTGGAATACAAAGATATCCCCGTGCCCAAACCCAAAGCCAATGAACTGCTGATCAACGTGAAATACAGTGGCGTGTGCCACACCGATCTGCACGCCTGGCATGGCGATTGGCCTCTGCCCGTGAAACTGCCCCTGGTGGGTGGCCATGAAGGCGCTGGCGTGGTGGTGGGCATGGGCGAAAACGTGAAAGGCTGGAAAATCGGCGATTACGCTGGCATCAAATGGCTGAACGGCAGCTGCATGGCCTGCGAATACTGCGAACTGGGCAACGAAAGCAACTGTCCCCACGCCGATCTGAGCGGCTACACCCACGATGGCAGCTTTCAGCAGTACGCCACCGCTGATGCCGTGCAGGCTGCCCACATTCCCCAGGGCACCGATCTGGCTCAGGTGGCTCCCATTCTGTGCGCTGGCATTACCGTGTACAAAGCCCTGAAAAGCGCCAACCTGATGGCTGGCCACTGGGTCGCCATTAGTGGCGCTGCTGGCGGTCTGGGCAGTCTGGCCGTGCAGTATGCCAAAGCCATGGGCTATCGCGTGCTGGGCATCGATGGTGGCGAAGGCAAAGAAGAACTGTTTCGCAGCATCGGTGGCGAAGTGTTTATCGATTTCACCAAAGAAAAAGATATCGTGGGTGCCGTCCTGAAAGCCACCGATGGCGGTGCTCACGGCGTGATCAACGTCAGCGTGAGCGAAGCCGCTATCGAAGCCAGCACCCGCTACGTTCGCGCTAATGGCACCACCGTGCTGGTCGGCATGCCTGCTGGCGCTAAATGCTGCTCGGATGTGTTTAACCAGGTGGTGAAAAGCATCAGCATCGTGGGCAGCTATGTGGGCAATCGCGCTGATACTCGCGAAGCCCTGGATTTTTTTGCTCGCGGTCTGGTGAAAAGCCCCATCAAAGTGGTGGGCCTGAGCACCCTGCCCGAAATCTACGAAAAAATGGAAAAAGGCCAGATCGTCGGTCGCTACGTGGTGGATACCAGCAAATAATAGGGATCC
+
#ATGTCGATCCCCGAAACCCAGAAAGGCGTGATCTTTTACGAAAGCCACGGCAAACTGGAATACAAAGATATCCCCGTGCCCAAACCCAAAGCCAATGAACTGCTGATCAACG#TGAAATACAGTGGCGTGTGCCACACCGATCTGCACGCCTGGCATGGCGATTGGCCTCTGCCCGTGAAACTGCCCCTGGTGGGTGGCCATGAAGGCGCTGGCGTGGTGGTGGG#CATGGGCGAAAACGTGAAAGGCTGGAAAATCGGCGATTACGCTGGCATCAAATGGCTGAACGGCAGCTGCATGGCCTGCGAATACTGCGAACTGGGCAACGAAAGCAACTGT#CCCCACGCCGATCTGAGCGGCTACACCCACGATGGCAGCTTTCAGCAGTACGCCACCGCTGATGCCGTGCAGGCTGCCCACATTCCCCAGGGCACCGATCTGGCTCAGGTGG#CTCCCATTCTGTGCGCTGGCATTACCGTGTACAAAGCCCTGAAAAGCGCCAACCTGATGGCTGGCCACTGGGTCGCCATTAGTGGCGCTGCTGGCGGTCTGGGCAGTCTGGC#CGTGCAGTATGCCAAAGCCATGGGCTATCGCGTGCTGGGCATCGATGGTGGCGAAGGCAAAGAAGAACTGTTTCGCAGCATCGGTGGCGAAGTGTTTATCGATTTCACCAAA#GAAAAAGATATCGTGGGTGCCGTCCTGAAAGCCACCGATGGCGGTGCTCACGGCGTGATCAACGTCAGCGTGAGCGAAGCCGCTATCGAAGCCAGCACCCGCTACGTTCGCG#CTAATGGCACCACCGTGCTGGTCGGCATGCCTGCTGGCGCTAAATGCTGCTCGGATGTGTTTAACCAGGTGGTGAAAAGCATCAGCATCGTGGGCAGCTATGTGGGCAATCG#CGCTGATACTCGCGAAGCCCTGGATTTTTTTGCTCGCGGTCTGGTGAAAAGCCCCATCAAAGTGGTGGGCCTGAGCACCCTGCCCGAAATCTACGAAAAAATGGAAAAAGGC#CAGATCGTCGGTCGCTACGTGGTGGATACCAGCAAATAATAGGGATCC
*Description of function
*Description of function
ADH is short for Alcohol dehydrogenase which can catalyse the generation of ethanol.  
ADH is short for Alcohol dehydrogenase which can catalyse the generation of ethanol.  
 +
#[[Image:aaaaa.jpg]]
*Acknowedgment of sources and references
*Acknowedgment of sources and references
Bennetzen JL and Hall BD(1982)The primary structure of the Saccharomyces cerevisae gene for alcohol dehydrogenase.J Biol Chem.257(6):3018-25
Bennetzen JL and Hall BD(1982)The primary structure of the Saccharomyces cerevisae gene for alcohol dehydrogenase.J Biol Chem.257(6):3018-25
Line 27: Line 28:
'''groESL'''
'''groESL'''
*Primary nucleaic acid sequence
*Primary nucleaic acid sequence
-
CAGTTATCAGTTATCAGTTGTCAGGTTATTTCCACTGTTCACTGTTCACTGATAACTGTTAACTGTCCACTGTTCGGAAACCCGTACACACCAAAACATTGCCATGCTAGGATGGTTTTCGGTAAATTAGCACTCAGGAGTGGAGAGTGCTAACTCTGGAGAAATTAAAT
+
#CAGTTATCAGTTATCAGTTGTCAGGTTATTTCCACTGTTCACTGTTCACTGATAACTGTTAACTGTCCACTGTTCGGAAACCCGTACACACCAAAACATTGCCATGCTAGGA#TGGTTTTCGGTAAATTAGCACTCAGGAGTGGAGAGTGCTAACTCTGGAGAAATTAAAT
*Description of function
*Description of function
heat-shock-responsive operon
heat-shock-responsive operon
Line 36: Line 37:
'''Rbc'''
'''Rbc'''
*Primary nucleaic acid sequence
*Primary nucleaic acid sequence
-
CAGTCAATGGAGAGCATTGCCATAAGTAAAGGCATCCCCTGCGTGATAAGATTACCTTCAGAAAACAGATAGTTGCTGGGTTATCGCAGATTTTTCTCGCAACCAAATAACTGTAAATAATAACTGTCTCTGGGGCGACGGTAGGCTTTATATTGCCAAATTTCGCCCGTGGGAGAAAGCTAGGCTATTCAATGTTTATGGAGGACTGACCTAG
+
#CAGTCAATGGAGAGCATTGCCATAAGTAAAGGCATCCCCTGCGTGATAAGATTACCTTCAGAAAACAGATAGTTGCTGGGTTATCGCAGATTTTTCTCGCAACCAAATAACT#GTAAATAATAACTGTCTCTGGGGCGACGGTAGGCTTTATATTGCCAAATTTCGCCCGTGGGAGAAAGCTAGGCTATTCAATGTTTATGGAGGACTGACCTAG
*Description of function
*Description of function
RuBisCO promoter
RuBisCO promoter
*Acknowedgment of sources and references
*Acknowedgment of sources and references
Mutating the Synechocystis sp. PCC6803 RuBisCO promoter as a means of controlling levels of gene expression in cyanobacteria
Mutating the Synechocystis sp. PCC6803 RuBisCO promoter as a means of controlling levels of gene expression in cyanobacteria

Revision as of 02:07, 20 October 2009

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Parts

PDC

  • Primary nucleaic acid sequence
  1. ATGAGCGAAATCACCCTGGGCAAATACCTGTTTGAACGCCTGAAACAGGTGAACGTGAACACCGTGTTTGGCCTGCCTGGCGATTTTAATCTGAGCCTGCTGGATAAAATCT#ACGAAGTGGAAGGCATGCGCTGGGCTGGCAACGCCAACGAACTGAACGCTGCCTACGCTGCCGATGGCTACGCTCGCATCAAAGGCATGAGCTGTATCATCACGACCTTTGG#CGTGGGCGAACTGAGCGCTCTGAACGGCATTGCTGGCAGCTACGCCGAACACGTGGGCGTGCTGCATGTGGTGGGCGTGCCCAGCATTAGCGCTCAGGCCAAACAGCTGCTG#CTGCACCATACCCTGGGCAACGGCGATTTTACCGTGTTTCACCGCATGAGCGCCAACATCAGCGAAACCACCGCCATGATCACCGATATCGCCACCGCTCCCGCTGAAATCG#ATCGCTGCATCCGCACCACCTACGTGACCCAACGCCCTGTGTATCTGGGCCTCCCTGCCAACCTGGTGGATCTGAACGTGCCTGCCAAACTGCTGCAGACCCCCATTGATAT#GAGCCTGAAACCCAACGATGCCGAAAGCGAAAAAGAAGTGATCGATACCATCCTGGCCCTGGTGAAAGATGCCAAAAACCCCGTGATCCTGGCCGATGCCTGCTGCAGTCGC#CACGATGTGAAAGCCGAAACCAAAAAACTGATCGATCTGACCCAGTTTCCCGCTTTTGTGACCCCCATGGGCAAAGGCAGCATCGATGAACAGCACCCTCGCTACGGTGGCG#TGTATGTGGGCACCCTGAGCAAACCCGAAGTGAAAGAAGCCGTGGAAAGCGCTGATCTGATCCTGAGCGTGGGTGCCCTGCTGTCGGATTTTAACACCGGCAGCTTTAGCTA#CAGCTACAAGACCAAAAACATCGTGGAATTTCACAGCGATCACATGAAAATCCGCAACGCCACCTTTCCTGGCGTGCAGATGAAATTTGTGCTGCAGAAACTGCTGACCACC#ATTGCCGATGCCGCTAAAGGCTACAAACCCGTGGCTGTTCCCGCTCGCACCCCTGCTAATGCTGCTGTGCCTGCCAGCACCCCTCTGAAACAAGAATGGATGTGGAACCAGC#TGGGCAACTTTCTGCAAGAGGGTGATGTGGTGATCGCCGAAACCGGCACCAGCGCCTTTGGCATCAACCAGACCACCTTTCCCAACAACACCTACGGCATCAGCCAGGTGCT#GTGGGGCAGCATCGGCTTTACGACCGGTGCTACCCTGGGTGCTGCGTTTGCCGCTGAAGAAATCGATCCCAAAAAACGCGTGATTCTGTTTATCGGTGATGGCAGCCTGCAG#CTGACCGTGCAAGAAATCAGCACCATGATCCGCTGGGGTCTGAAACCCTACCTGTTTGTGCTGAATAACGATGGCTACACCATCGAAAAACTGATCCACGGTCCCAAAGCCC#AGTACAACGAAATCCAGGGCTGGGATCACCTGAGTCTGCTGCCGACCTTTGGCGCTAAAGATTACGAAACCCATCGCGTGGCTACCACGGGTGAATGGGATAAACTGACCCA#GGATAAGAGCTTTAACGATAACAGCAAGATCCGCATGATCGAAATCATGCTGCCCGTGTTTGATGCTCCCCAGAACCTGGTGGAACAGGCCAAACTGACCGCTGCCACCAAC#GCCAAACAGTAA
  • Description of function

PDC is short for pyruvate decarboxylase, it is a homotetrameric enzyme Pyruvate decarboxylase which can converting pyruvate into acetaldehyde and carbon dioxide.

  • Acknowedgment of sources and references

Kellermann,E., Seeboth,P.G. and Hollenberg,C.P. Analysis of the primary structure and promoter function of a pyruvate decarboxylase gene (PDC1) from Saccharomyces cerevisiae, Nucleic Acids Res. 14 (22), 8963-8977 (1986)


ADH

  • Primary nucleaic acid sequence
  1. ATGTCGATCCCCGAAACCCAGAAAGGCGTGATCTTTTACGAAAGCCACGGCAAACTGGAATACAAAGATATCCCCGTGCCCAAACCCAAAGCCAATGAACTGCTGATCAACG#TGAAATACAGTGGCGTGTGCCACACCGATCTGCACGCCTGGCATGGCGATTGGCCTCTGCCCGTGAAACTGCCCCTGGTGGGTGGCCATGAAGGCGCTGGCGTGGTGGTGGG#CATGGGCGAAAACGTGAAAGGCTGGAAAATCGGCGATTACGCTGGCATCAAATGGCTGAACGGCAGCTGCATGGCCTGCGAATACTGCGAACTGGGCAACGAAAGCAACTGT#CCCCACGCCGATCTGAGCGGCTACACCCACGATGGCAGCTTTCAGCAGTACGCCACCGCTGATGCCGTGCAGGCTGCCCACATTCCCCAGGGCACCGATCTGGCTCAGGTGG#CTCCCATTCTGTGCGCTGGCATTACCGTGTACAAAGCCCTGAAAAGCGCCAACCTGATGGCTGGCCACTGGGTCGCCATTAGTGGCGCTGCTGGCGGTCTGGGCAGTCTGGC#CGTGCAGTATGCCAAAGCCATGGGCTATCGCGTGCTGGGCATCGATGGTGGCGAAGGCAAAGAAGAACTGTTTCGCAGCATCGGTGGCGAAGTGTTTATCGATTTCACCAAA#GAAAAAGATATCGTGGGTGCCGTCCTGAAAGCCACCGATGGCGGTGCTCACGGCGTGATCAACGTCAGCGTGAGCGAAGCCGCTATCGAAGCCAGCACCCGCTACGTTCGCG#CTAATGGCACCACCGTGCTGGTCGGCATGCCTGCTGGCGCTAAATGCTGCTCGGATGTGTTTAACCAGGTGGTGAAAAGCATCAGCATCGTGGGCAGCTATGTGGGCAATCG#CGCTGATACTCGCGAAGCCCTGGATTTTTTTGCTCGCGGTCTGGTGAAAAGCCCCATCAAAGTGGTGGGCCTGAGCACCCTGCCCGAAATCTACGAAAAAATGGAAAAAGGC#CAGATCGTCGGTCGCTACGTGGTGGATACCAGCAAATAATAGGGATCC
  • Description of function

ADH is short for Alcohol dehydrogenase which can catalyse the generation of ethanol.

  1. Aaaaa.jpg
  • Acknowedgment of sources and references

Bennetzen JL and Hall BD(1982)The primary structure of the Saccharomyces cerevisae gene for alcohol dehydrogenase.J Biol Chem.257(6):3018-25


groESL

  • Primary nucleaic acid sequence
  1. CAGTTATCAGTTATCAGTTGTCAGGTTATTTCCACTGTTCACTGTTCACTGATAACTGTTAACTGTCCACTGTTCGGAAACCCGTACACACCAAAACATTGCCATGCTAGGA#TGGTTTTCGGTAAATTAGCACTCAGGAGTGGAGAGTGCTAACTCTGGAGAAATTAAAT
  • Description of function

heat-shock-responsive operon

  • Acknowedgment of sources and references

Cloning and characterization of the major groESL operon from a nitrogen-¢xing cyanobacterium Anabaena sp. strain L-31


Rbc

  • Primary nucleaic acid sequence
  1. CAGTCAATGGAGAGCATTGCCATAAGTAAAGGCATCCCCTGCGTGATAAGATTACCTTCAGAAAACAGATAGTTGCTGGGTTATCGCAGATTTTTCTCGCAACCAAATAACT#GTAAATAATAACTGTCTCTGGGGCGACGGTAGGCTTTATATTGCCAAATTTCGCCCGTGGGAGAAAGCTAGGCTATTCAATGTTTATGGAGGACTGACCTAG
  • Description of function

RuBisCO promoter

  • Acknowedgment of sources and references

Mutating the Synechocystis sp. PCC6803 RuBisCO promoter as a means of controlling levels of gene expression in cyanobacteria

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