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=== Parts ===
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'''PDC'''
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*Primary nucleaic acid sequence
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ATGAGCGAAATCACCCTGGGCAAATACCTGTTTGAACGCCTGAAACAGGTGAACGTGAACACCGTGTTTGGCCTGCCTGGCGATTTTAATCTGAGCCTGCTGGATAAAATCTACGAAGTGGAAGGCATGCGCTGGGCTGGCAACGCCAACGAACTGAACGCTGCCTACGCTGCCGATGGCTACGCTCGCATCAAAGGCATGAGCTGTATCATCACGACCTTTGGCGTGGGCGAACTGAGCGCTCTGAACGGCATTGCTGGCAGCTACGCCGAACACGTGGGCGTGCTGCATGTGGTGGGCGTGCCCAGCATTAGCGCTCAGGCCAAACAGCTGCTGCTGCACCATACCCTGGGCAACGGCGATTTTACCGTGTTTCACCGCATGAGCGCCAACATCAGCGAAACCACCGCCATGATCACCGATATCGCCACCGCTCCCGCTGAAATCGATCGCTGCATCCGCACCACCTACGTGACCCAACGCCCTGTGTATCTGGGCCTCCCTGCCAACCTGGTGGATCTGAACGTGCCTGCCAAACTGCTGCAGACCCCCATTGATATGAGCCTGAAACCCAACGATGCCGAAAGCGAAAAAGAAGTGATCGATACCATCCTGGCCCTGGTGAAAGATGCCAAAAACCCCGTGATCCTGGCCGATGCCTGCTGCAGTCGCCACGATGTGAAAGCCGAAACCAAAAAACTGATCGATCTGACCCAGTTTCCCGCTTTTGTGACCCCCATGGGCAAAGGCAGCATCGATGAACAGCACCCTCGCTACGGTGGCGTGTATGTGGGCACCCTGAGCAAACCCGAAGTGAAAGAAGCCGTGGAAAGCGCTGATCTGATCCTGAGCGTGGGTGCCCTGCTGTCGGATTTTAACACCGGCAGCTTTAGCTACAGCTACAAGACCAAAAACATCGTGGAATTTCACAGCGATCACATGAAAATCCGCAACGCCACCTTTCCTGGCGTGCAGATGAAATTTGTGCTGCAGAAACTGCTGACCACCATTGCCGATGCCGCTAAAGGCTACAAACCCGTGGCTGTTCCCGCTCGCACCCCTGCTAATGCTGCTGTGCCTGCCAGCACCCCTCTGAAACAAGAATGGATGTGGAACCAGCTGGGCAACTTTCTGCAAGAGGGTGATGTGGTGATCGCCGAAACCGGCACCAGCGCCTTTGGCATCAACCAGACCACCTTTCCCAACAACACCTACGGCATCAGCCAGGTGCTGTGGGGCAGCATCGGCTTTACGACCGGTGCTACCCTGGGTGCTGCGTTTGCCGCTGAAGAAATCGATCCCAAAAAACGCGTGATTCTGTTTATCGGTGATGGCAGCCTGCAGCTGACCGTGCAAGAAATCAGCACCATGATCCGCTGGGGTCTGAAACCCTACCTGTTTGTGCTGAATAACGATGGCTACACCATCGAAAAACTGATCCACGGTCCCAAAGCCCAGTACAACGAAATCCAGGGCTGGGATCACCTGAGTCTGCTGCCGACCTTTGGCGCTAAAGATTACGAAACCCATCGCGTGGCTACCACGGGTGAATGGGATAAACTGACCCAGGATAAGAGCTTTAACGATAACAGCAAGATCCGCATGATCGAAATCATGCTGCCCGTGTTTGATGCTCCCCAGAACCTGGTGGAACAGGCCAAACTGACCGCTGCCACCAACGCCAAACAGTAA
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*Description of function
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PDC is short for pyruvate decarboxylase, it is a homotetrameric enzyme Pyruvate decarboxylase which can converting pyruvate into acetaldehyde and carbon dioxide.
 +
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*Acknowedgment of sources and references
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Kellermann,E., Seeboth,P.G. and Hollenberg,C.P.
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Analysis of the primary structure and promoter function of a pyruvate decarboxylase gene (PDC1) from Saccharomyces cerevisiae, Nucleic Acids Res. 14 (22), 8963-8977 (1986)
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'''ADH'''
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*Primary nucleaic acid sequence
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ATGTCGATCCCCGAAACCCAGAAAGGCGTGATCTTTTACGAAAGCCACGGCAAACTGGAATACAAAGATATCCCCGTGCCCAAACCCAAAGCCAATGAACTGCTGATCAACGTGAAATACAGTGGCGTGTGCCACACCGATCTGCACGCCTGGCATGGCGATTGGCCTCTGCCCGTGAAACTGCCCCTGGTGGGTGGCCATGAAGGCGCTGGCGTGGTGGTGGGCATGGGCGAAAACGTGAAAGGCTGGAAAATCGGCGATTACGCTGGCATCAAATGGCTGAACGGCAGCTGCATGGCCTGCGAATACTGCGAACTGGGCAACGAAAGCAACTGTCCCCACGCCGATCTGAGCGGCTACACCCACGATGGCAGCTTTCAGCAGTACGCCACCGCTGATGCCGTGCAGGCTGCCCACATTCCCCAGGGCACCGATCTGGCTCAGGTGGCTCCCATTCTGTGCGCTGGCATTACCGTGTACAAAGCCCTGAAAAGCGCCAACCTGATGGCTGGCCACTGGGTCGCCATTAGTGGCGCTGCTGGCGGTCTGGGCAGTCTGGCCGTGCAGTATGCCAAAGCCATGGGCTATCGCGTGCTGGGCATCGATGGTGGCGAAGGCAAAGAAGAACTGTTTCGCAGCATCGGTGGCGAAGTGTTTATCGATTTCACCAAAGAAAAAGATATCGTGGGTGCCGTCCTGAAAGCCACCGATGGCGGTGCTCACGGCGTGATCAACGTCAGCGTGAGCGAAGCCGCTATCGAAGCCAGCACCCGCTACGTTCGCGCTAATGGCACCACCGTGCTGGTCGGCATGCCTGCTGGCGCTAAATGCTGCTCGGATGTGTTTAACCAGGTGGTGAAAAGCATCAGCATCGTGGGCAGCTATGTGGGCAATCGCGCTGATACTCGCGAAGCCCTGGATTTTTTTGCTCGCGGTCTGGTGAAAAGCCCCATCAAAGTGGTGGGCCTGAGCACCCTGCCCGAAATCTACGAAAAAATGGAAAAAGGCCAGATCGTCGGTCGCTACGTGGTGGATACCAGCAAATAATAGGGATCC
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*Description of function
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ADH is short for Alcohol dehydrogenase which can catalyse the generation of ethanol.
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*Acknowedgment of sources and references
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Bennetzen JL and Hall BD(1982)The primary structure of the Saccharomyces cerevisae gene for alcohol dehydrogenase.J Biol Chem.257(6):3018-25
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'''groESL'''
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*Primary nucleaic acid sequence
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CAGTTATCAGTTATCAGTTGTCAGGTTATTTCCACTGTTCACTGTTCACTGATAACTGTTAACTGTCCACTGTTCGGAAACCCGTACACACCAAAACATTGCCATGCTAGGATGGTTTTCGGTAAATTAGCACTCAGGAGTGGAGAGTGCTAACTCTGGAGAAATTAAAT
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*Description of function
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heat-shock-responsive operon
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*Acknowedgment of sources and references
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Cloning and characterization of the major groESL operon from a nitrogen-¢xing cyanobacterium Anabaena sp. strain L-31
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'''Rbc'''
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*Primary nucleaic acid sequence
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CAGTCAATGGAGAGCATTGCCATAAGTAAAGGCATCCCCTGCGTGATAAGATTACCTTCAGAAAACAGATAGTTGCTGGGTTATCGCAGATTTTTCTCGCAACCAAATAACTGTAAATAATAACTGTCTCTGGGGCGACGGTAGGCTTTATATTGCCAAATTTCGCCCGTGGGAGAAAGCTAGGCTATTCAATGTTTATGGAGGACTGACCTAG
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*Description of function
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RuBisCO promoter
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*Acknowedgment of sources and references
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Mutating the Synechocystis sp. PCC6803 RuBisCO promoter as a means of controlling levels of gene expression in cyanobacteria

Revision as of 02:03, 20 October 2009

Tju.jpg

Home|Project|Team |Protocols Parts Modeling |Gallery |Notebook|Ethics|

Parts

PDC

  • Primary nucleaic acid sequence

ATGAGCGAAATCACCCTGGGCAAATACCTGTTTGAACGCCTGAAACAGGTGAACGTGAACACCGTGTTTGGCCTGCCTGGCGATTTTAATCTGAGCCTGCTGGATAAAATCTACGAAGTGGAAGGCATGCGCTGGGCTGGCAACGCCAACGAACTGAACGCTGCCTACGCTGCCGATGGCTACGCTCGCATCAAAGGCATGAGCTGTATCATCACGACCTTTGGCGTGGGCGAACTGAGCGCTCTGAACGGCATTGCTGGCAGCTACGCCGAACACGTGGGCGTGCTGCATGTGGTGGGCGTGCCCAGCATTAGCGCTCAGGCCAAACAGCTGCTGCTGCACCATACCCTGGGCAACGGCGATTTTACCGTGTTTCACCGCATGAGCGCCAACATCAGCGAAACCACCGCCATGATCACCGATATCGCCACCGCTCCCGCTGAAATCGATCGCTGCATCCGCACCACCTACGTGACCCAACGCCCTGTGTATCTGGGCCTCCCTGCCAACCTGGTGGATCTGAACGTGCCTGCCAAACTGCTGCAGACCCCCATTGATATGAGCCTGAAACCCAACGATGCCGAAAGCGAAAAAGAAGTGATCGATACCATCCTGGCCCTGGTGAAAGATGCCAAAAACCCCGTGATCCTGGCCGATGCCTGCTGCAGTCGCCACGATGTGAAAGCCGAAACCAAAAAACTGATCGATCTGACCCAGTTTCCCGCTTTTGTGACCCCCATGGGCAAAGGCAGCATCGATGAACAGCACCCTCGCTACGGTGGCGTGTATGTGGGCACCCTGAGCAAACCCGAAGTGAAAGAAGCCGTGGAAAGCGCTGATCTGATCCTGAGCGTGGGTGCCCTGCTGTCGGATTTTAACACCGGCAGCTTTAGCTACAGCTACAAGACCAAAAACATCGTGGAATTTCACAGCGATCACATGAAAATCCGCAACGCCACCTTTCCTGGCGTGCAGATGAAATTTGTGCTGCAGAAACTGCTGACCACCATTGCCGATGCCGCTAAAGGCTACAAACCCGTGGCTGTTCCCGCTCGCACCCCTGCTAATGCTGCTGTGCCTGCCAGCACCCCTCTGAAACAAGAATGGATGTGGAACCAGCTGGGCAACTTTCTGCAAGAGGGTGATGTGGTGATCGCCGAAACCGGCACCAGCGCCTTTGGCATCAACCAGACCACCTTTCCCAACAACACCTACGGCATCAGCCAGGTGCTGTGGGGCAGCATCGGCTTTACGACCGGTGCTACCCTGGGTGCTGCGTTTGCCGCTGAAGAAATCGATCCCAAAAAACGCGTGATTCTGTTTATCGGTGATGGCAGCCTGCAGCTGACCGTGCAAGAAATCAGCACCATGATCCGCTGGGGTCTGAAACCCTACCTGTTTGTGCTGAATAACGATGGCTACACCATCGAAAAACTGATCCACGGTCCCAAAGCCCAGTACAACGAAATCCAGGGCTGGGATCACCTGAGTCTGCTGCCGACCTTTGGCGCTAAAGATTACGAAACCCATCGCGTGGCTACCACGGGTGAATGGGATAAACTGACCCAGGATAAGAGCTTTAACGATAACAGCAAGATCCGCATGATCGAAATCATGCTGCCCGTGTTTGATGCTCCCCAGAACCTGGTGGAACAGGCCAAACTGACCGCTGCCACCAACGCCAAACAGTAA

  • Description of function

PDC is short for pyruvate decarboxylase, it is a homotetrameric enzyme Pyruvate decarboxylase which can converting pyruvate into acetaldehyde and carbon dioxide.

  • Acknowedgment of sources and references

Kellermann,E., Seeboth,P.G. and Hollenberg,C.P. Analysis of the primary structure and promoter function of a pyruvate decarboxylase gene (PDC1) from Saccharomyces cerevisiae, Nucleic Acids Res. 14 (22), 8963-8977 (1986)


ADH

  • Primary nucleaic acid sequence

ATGTCGATCCCCGAAACCCAGAAAGGCGTGATCTTTTACGAAAGCCACGGCAAACTGGAATACAAAGATATCCCCGTGCCCAAACCCAAAGCCAATGAACTGCTGATCAACGTGAAATACAGTGGCGTGTGCCACACCGATCTGCACGCCTGGCATGGCGATTGGCCTCTGCCCGTGAAACTGCCCCTGGTGGGTGGCCATGAAGGCGCTGGCGTGGTGGTGGGCATGGGCGAAAACGTGAAAGGCTGGAAAATCGGCGATTACGCTGGCATCAAATGGCTGAACGGCAGCTGCATGGCCTGCGAATACTGCGAACTGGGCAACGAAAGCAACTGTCCCCACGCCGATCTGAGCGGCTACACCCACGATGGCAGCTTTCAGCAGTACGCCACCGCTGATGCCGTGCAGGCTGCCCACATTCCCCAGGGCACCGATCTGGCTCAGGTGGCTCCCATTCTGTGCGCTGGCATTACCGTGTACAAAGCCCTGAAAAGCGCCAACCTGATGGCTGGCCACTGGGTCGCCATTAGTGGCGCTGCTGGCGGTCTGGGCAGTCTGGCCGTGCAGTATGCCAAAGCCATGGGCTATCGCGTGCTGGGCATCGATGGTGGCGAAGGCAAAGAAGAACTGTTTCGCAGCATCGGTGGCGAAGTGTTTATCGATTTCACCAAAGAAAAAGATATCGTGGGTGCCGTCCTGAAAGCCACCGATGGCGGTGCTCACGGCGTGATCAACGTCAGCGTGAGCGAAGCCGCTATCGAAGCCAGCACCCGCTACGTTCGCGCTAATGGCACCACCGTGCTGGTCGGCATGCCTGCTGGCGCTAAATGCTGCTCGGATGTGTTTAACCAGGTGGTGAAAAGCATCAGCATCGTGGGCAGCTATGTGGGCAATCGCGCTGATACTCGCGAAGCCCTGGATTTTTTTGCTCGCGGTCTGGTGAAAAGCCCCATCAAAGTGGTGGGCCTGAGCACCCTGCCCGAAATCTACGAAAAAATGGAAAAAGGCCAGATCGTCGGTCGCTACGTGGTGGATACCAGCAAATAATAGGGATCC

  • Description of function

ADH is short for Alcohol dehydrogenase which can catalyse the generation of ethanol.

  • Acknowedgment of sources and references

Bennetzen JL and Hall BD(1982)The primary structure of the Saccharomyces cerevisae gene for alcohol dehydrogenase.J Biol Chem.257(6):3018-25


groESL

  • Primary nucleaic acid sequence

CAGTTATCAGTTATCAGTTGTCAGGTTATTTCCACTGTTCACTGTTCACTGATAACTGTTAACTGTCCACTGTTCGGAAACCCGTACACACCAAAACATTGCCATGCTAGGATGGTTTTCGGTAAATTAGCACTCAGGAGTGGAGAGTGCTAACTCTGGAGAAATTAAAT

  • Description of function

heat-shock-responsive operon

  • Acknowedgment of sources and references

Cloning and characterization of the major groESL operon from a nitrogen-¢xing cyanobacterium Anabaena sp. strain L-31


Rbc

  • Primary nucleaic acid sequence

CAGTCAATGGAGAGCATTGCCATAAGTAAAGGCATCCCCTGCGTGATAAGATTACCTTCAGAAAACAGATAGTTGCTGGGTTATCGCAGATTTTTCTCGCAACCAAATAACTGTAAATAATAACTGTCTCTGGGGCGACGGTAGGCTTTATATTGCCAAATTTCGCCCGTGGGAGAAAGCTAGGCTATTCAATGTTTATGGAGGACTGACCTAG

  • Description of function

RuBisCO promoter

  • Acknowedgment of sources and references

Mutating the Synechocystis sp. PCC6803 RuBisCO promoter as a means of controlling levels of gene expression in cyanobacteria

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