SNOWDRIFT/Proof of concept

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'''Proof of Concept:'''
In order to establish cell-cell communication protein signalling can come in handy.
In order to establish cell-cell communication protein signalling can come in handy.

Revision as of 07:05, 21 October 2009

Proof of Concept:

In order to establish cell-cell communication protein signalling can come in handy. We attempted to do this by attaching what we call a 'secretion tag' to our protein of interest. This 'tag' allows secretion of proteins.

This tag is designed by using N-terminal sequences from proteins which are exported using the TAT Pathway.

We tried to test this export mechanism using the following biobricks:

Construct11.JPG

Both these constructs are designed to secrete the Green Fluorescent Protein in to the extracellular environment.