SNOWDRIFT/Proof of concept

From 2009.igem.org

(Difference between revisions)
Line 7: Line 7:
We tried to test this export mechanism using the following biobricks:
We tried to test this export mechanism using the following biobricks:
-
[[Image:700px-Construct11.JPG|950 px]]
+
<br>
 +
[[Image:Snowdriftproofofconcept.jpeg]]
 +
<br>
Both these constructs are designed to secrete the Green Fluorescent Protein in to the extracellular environment.
Both these constructs are designed to secrete the Green Fluorescent Protein in to the extracellular environment.

Revision as of 14:16, 21 October 2009

Proof of Concept:

In order to establish cell-cell communication protein signalling can come in handy. We attempted to do this by attaching what we call a 'secretion tag' to our protein of interest. This 'tag' allows secretion of proteins.

This tag is designed by using N-terminal sequences from proteins which are exported using the TAT Pathway.

We tried to test this export mechanism using the following biobricks:
File:Snowdriftproofofconcept.jpeg
Both these constructs are designed to secrete the Green Fluorescent Protein in to the extracellular environment.