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From 2009.igem.org

Combined display of all available logs of 2009.igem.org. You can narrow down the view by selecting a log type, the user name (case-sensitive), or the affected page (also case-sensitive).

13:45, 13 October 2009 Wilfred (Talk | contribs) uploaded "File:Eye Vision.png" ‎
13:43, 13 October 2009 Wilfred (Talk | contribs) uploaded "File:Balloon Communication.png" ‎
13:08, 13 October 2009 Wilfred (Talk | contribs) uploaded "File:Hardhat Safety.png" ‎
09:05, 13 October 2009 Wilfred (Talk | contribs) uploaded "File:Human Practice Balance.png" ‎
11:01, 8 October 2009 Wilfred (Talk | contribs) uploaded "File:Cloning MT Transporter in AC3-new.tif" ‎
10:52, 8 October 2009 Wilfred (Talk | contribs) uploaded a new version of "File:Cloning MT Transporter in AC3-new.pdf" ‎
14:53, 29 September 2009 Wilfred (Talk | contribs) uploaded "File:Fermentation set-up zoom.JPG" ‎
14:51, 29 September 2009 Wilfred (Talk | contribs) uploaded "File:Fermentation set-up.JPG" ‎ (Our beautiful fermentation set-up)
18:54, 25 September 2009 Wilfred (Talk | contribs) uploaded "File:IGEMGroningen Molen.jpg" ‎ (Pure Dutch scenery, can it get anymore stereotypical?)
19:18, 10 September 2009 Wilfred (Talk | contribs) uploaded a new version of "File:Allignment MBP-ArsR.jpg" ‎ (A Tobacco Etch Virus (TEV) protease cleavage site is added between the two proteins.)
19:12, 10 September 2009 Wilfred (Talk | contribs) uploaded "File:Allignment MBP-ArsR.jpg" ‎ (Allignments of the metal chelator Maltose Binding Protein (MBP)-ArsR fusion protein (<partinfo>BBa_K190027</partinfo>). Above is <i>In silico</i> construct, in the middle is sequence determined with VF2 primer, below the sequence determined with VR primer)
18:37, 10 September 2009 Wilfred (Talk | contribs) uploaded "File:Allignments fMT.jpg" ‎ (Allignments of the metallothionein fMT constructs. The first is without promotor (BBa_K190019), second with constitutive promotor BBa_J23109 (BBa_K190031) and the third with IPTG inducible promotor BBa_R0010 (BBa_K190032). RBS is BBa_B0034. In combining t)
12:01, 10 September 2009 Wilfred (Talk | contribs) uploaded a new version of "File:Next.JPG" ‎
11:41, 10 September 2009 Wilfred (Talk | contribs) uploaded "File:Next.JPG" ‎
13:34, 8 September 2009 Wilfred (Talk | contribs) uploaded "File:PCR of SmtA - 08september2009.jpg" ‎
15:40, 5 September 2009 Wilfred (Talk | contribs) uploaded "File:Restriction analysis SmtA (pSB1AC3) with XbaI & PstI- 05september2009.jpg" ‎ (Restriction analysis of SmtA colony #1 using XbaI & PstI. Expected fragment was ~700 bp. )
15:05, 5 September 2009 Wilfred (Talk | contribs) uploaded "File:Colony PCR of SmtA-1 - 05september2009.jpg" ‎ (Colony PCR of SmtA colony #1 using Biobrick VF2/VR primers (lane 1) and RBSforward and SmtAreverse primers (lane 2). Probably a different RBS primer was used, explaining the lack of product. SmtA (including RBS) is 678 bp, PCR product using VF2/VR primers)
16:02, 2 September 2009 Wilfred (Talk | contribs) uploaded "File:Colony PCR SmtA pLac (pSB1A2) - 02september2009.jpg" ‎
07:35, 2 September 2009 Wilfred (Talk | contribs) uploaded "File:Restriction analysis SmtA pLow (pSB1AC3) - 01september2009.jpg" ‎ (Restriction analysis of the metallothionein SmtA with a low constitutive promotor. Restriction analysis was performed using XbaI & PstI, SmtA+pLow should give a band of ~750 bp, pSB1AC3 ~3000 bp. The lowest band is at a height of ~1000 bp.)
12:37, 1 September 2009 Wilfred (Talk | contribs) uploaded "File:Colony PCR SmtA pLow&pLac (pSB1A2) - 31august2009.jpg" ‎
14:10, 27 August 2009 Wilfred (Talk | contribs) uploaded "File:FMT & MBP-ArsR colony PCR to check presence inducible promotors - 27august2009.JPG" ‎
12:29, 25 August 2009 Wilfred (Talk | contribs) uploaded "File:PCR Check MBP-ArsR - 24august2009.jpg" ‎
15:16, 19 August 2009 Wilfred (Talk | contribs) uploaded a new version of "File:19august2009 Float test with GVP plasmid under Metal promotors -2.jpg" ‎
15:15, 19 August 2009 Wilfred (Talk | contribs) uploaded a new version of "File:19august2009 Float test with GVP plasmid under Metal promotors -1.jpg" ‎
15:01, 19 August 2009 Wilfred (Talk | contribs) uploaded "File:19august2009 Float test with GVP plasmid under Metal promotors -1.jpg" ‎
15:01, 19 August 2009 Wilfred (Talk | contribs) uploaded "File:19august2009 Float test with GVP plasmid under Metal promotors -2.jpg" ‎
11:15, 19 August 2009 Wilfred (Talk | contribs) uploaded "File:Metal promotors in pSB1AC3 or pSB3K3 cut with SpeI & EcoRI - 18august2009.jpg" ‎ (Restriction analysis of plasmids engineered to contain metal promotors. Constructs were digested using SpeI & EcoRI, expecting to cut out the ~100 bp that is the promotor. Gel is 2% agarose (1xTBE). DNA marker is 1 Kb.)
13:41, 18 August 2009 Wilfred (Talk | contribs) uploaded "File:MBP-ArsR Fusion protein in pSB1AC3 restricted with XbaI & EcoRI - 18august2009.jpg" ‎ (1% Agarose (1xTBE) gel of MBP-ArsR fusion proteins, with selfcloser (1), sample (white colony) (2), sample (red colony??) (3). Expected sizes of positive construct are shown. Samples were digested using PstI & XbaI, ~45 min 37 C.)
15:20, 17 August 2009 Wilfred (Talk | contribs) uploaded "File:17august2009 Float test with GVP plasmid.jpg" ‎ (Preliminary test with Gas Vesicle Proteins induced by IPTG on plate. Picture is after three days.)
09:29, 16 August 2009 Wilfred (Talk | contribs) uploaded "File:16august2009 Float test with GVP plasmid.jpg" ‎ (Preliminary test with Gas Vesicle Proteins induced by IPTG on plate. Picture is after two days.)
10:24, 15 August 2009 Wilfred (Talk | contribs) uploaded "File:15august2009 Float test with GVP plasmid.jpg" ‎ (Preliminary test with Gas Vesicle Proteins expressed constitutively. Picture is after one day.)
13:18, 7 August 2009 Wilfred (Talk | contribs) uploaded "File:PSB1AC3-GVP-metalpromotors restricted with NotI - 7august2009.jpg" ‎
18:15, 5 August 2009 Wilfred (Talk | contribs) uploaded "File:Inside Brochure iGEM GRONINGEN.jpg" ‎
18:13, 5 August 2009 Wilfred (Talk | contribs) uploaded "File:Outside Brochure iGEM GRONINGEN.jpg" ‎
19:46, 30 July 2009 Wilfred (Talk | contribs) uploaded "File:PSB1AC3 & pSB3K3 cut with SpeI & EcoRI.jpg" ‎ (1% agarose gel with vectors cut with SpeI & EcoRI. pSB3K3 vector was diluted too much, so not visible on gel.)
09:13, 29 July 2009 Wilfred (Talk | contribs) uploaded "File:PSB3K3 restricted with EcoRI & SpeI - 28july2009.JPG" ‎ (1% agarose gel showing pSB3K3 vector cut with SpeI & EcoRI. This was used for a gel purification.)
09:10, 29 July 2009 Wilfred (Talk | contribs) uploaded "File:PSB1AC3 restricted with EcoRi & SpeI - 28july2009.JPG" ‎ (Gel showing succesful restriction used for gel purification)
19:50, 24 June 2009 Wilfred (Talk | contribs) uploaded "File:The city of Talent.jpg" ‎ (-,-)
19:32, 24 June 2009 Wilfred (Talk | contribs) uploaded a new version of "File:The city of Talent.pdf" ‎ (JPEG)
19:27, 24 June 2009 Wilfred (Talk | contribs) uploaded "File:The city of Talent.pdf" ‎ (One of the sponsors of iGEM Groningen.)
18:50, 12 June 2009 Wilfred (Talk | contribs) uploaded a new version of "File:Wilfred Poppinga.jpg" ‎
15:26, 1 May 2009 Wilfred (Talk | contribs) uploaded "File:Wilfred Poppinga.jpg" ‎ (Personal picture)