http://2009.igem.org/wiki/index.php?title=Special:Contributions&feed=atom&limit=500&target=Meagan&year=&month=2009.igem.org - User contributions [en]2024-03-29T06:59:41ZFrom 2009.igem.orgMediaWiki 1.16.5http://2009.igem.org/JamboreeJamboree2010-11-09T23:22:35Z<p>Meagan: /* Contact Information */</p>
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<span style="color:#576f91; font-family:georgia, serif; align:center; font-weight:bold; padding-left:40px; margin-bottom: 125px;">The iGEM 2009 Jamboree will take place on October 30th to November 2nd, 2009 at [http://whereis-beta.mit.edu/?mapterms=stata%20center&zoom=15&lat=42.36161990569666&lng=-71.09055519104004&open=object-32 MIT Stata Center] and [http://whereis-beta.mit.edu/?mapterms=lobby%2013&zoom=15&lat=42.35993922977393&lng=-71.092529296875&open=object-13 Lobby 13] in Cambridge, MA.</span><br />
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<strong><a class="heading" href="https://2009.igem.org/Jamboree#Pre-Jamboree">Pre-Jamboree</a></strong><br />
<ul style="list-style:none;"><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Jamboree_Registration">Register for the Jamboree</a> : <span class="deadline">September 18th</span></li><br />
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<li><a class="green" href="https://2009.igem.org/Jamboree#Project_Abstract">Submit your project abstract</a> : <span class="deadline">September 18th</span></li><br />
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<li><a class="green" href="https://2009.igem.org/Jamboree#Team_Roster">Submit your team roster</a> : <span class="deadline">September 18th</span></li><br />
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<li><a class="green" href="https://2009.igem.org/Jamboree#Requests_for_Variance">Requests for variance</a> : <span class="deadline">September 18th</span></li><br />
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<li><a class="green" href="https://2009.igem.org/Jamboree#Tracks">Select your track</a> : <span class="deadline">September 18th</span></li><br />
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<li><a class="green" href="https://2009.igem.org/Jamboree#Visa_Letters">Request your visa letter</a> : <span class="deadline">June 1st</span></li><br />
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<li><a class="green" href="https://2009.igem.org/Jamboree#Documentation">Document your project</a> : <span class="deadline">October 21st</span></li><br />
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<li><a class="green" href="https://2009.igem.org/Jamboree#Documentation">Document your parts</a> : <span class="deadline">October 21st</span></li><br />
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<li><a class="green" href="https://2009.igem.org/Jamboree#DNA_Submissions">Promote your favorite parts</a> : <span class="deadline">October 21st</span></li><br />
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<li><a class="green" href="https://2009.igem.org/Jamboree#DNA_Submissions">Send in your parts</a> : <span class="deadline">October 21st</span></li><br />
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<li><a class="green" href="https://2009.igem.org/Jamboree#Poster_Requirements">Make your poster</a></li><br />
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<li><a class="green" href="https://2009.igem.org/Jamboree#Presentation_Requirements">Prepare your presentation</a></li><br />
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<li><a class="green" href="https://2009.igem.org/Jamboree#Software_Tools_Track">Software Tools Track Teams</a></li><br />
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<li><a class="green" href="https://2009.igem.org/Jamboree#Experimental_Track">Experimental Track Teams</a></li><br />
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<li><a class="green" href="https://2009.igem.org/Jamboree#Photo_Release_Form">Print, sign, and bring your photo release form</a></li><br />
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<li><a class="green" href="https://2009.igem.org/Jamboree#Hotels">Book your hotel</a></li><br />
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<li><a class="green" href="https://2009.igem.org/Jamboree#Guests">Guests, family, and friends</a></li><br />
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<li><a class="green" href="https://2009.igem.org/Jamboree#Volunteer">Volunteer for the Jamboree!</a></li><br />
</ul><br />
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<strong><a class="heading" href="https://2009.igem.org/Jamboree#During_the_Jamboree">During the Jamboree</a></strong><br />
<ul style="list-style:none;"><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Schedule">2009 Jamboree Schedule</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Check-In">Check-In and Registration</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Badges">You should always have your badge!</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Friday_Night_Practice">Friday Night Practice</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Photo_Release">Bring your photo release forms!</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Team_Leaders">Team Leader Responsibilities</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Team_Boxes">What's in Your Team Box</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Posters">Show off your posters!</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#iGEM_around_MIT_campus">iGEM around MIT campus</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Presentations">Giving your presentations</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#We_need_your_posters_&_presentations!">We need your posters & presentations!</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#iGEM_from_Above">iGEM from Above</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Medals">Medals and Awards!</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#iGEM_Wrap-up">It's over: The iGEM Wrap-up!</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Family_and_Friends">Family, Friends, and Guests</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Food">Food!</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Social_Event:_Sunday_night">The iGEM Social Event: Sunday night</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Compton Lounge">What's at the Compton Lounge</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#iGEM Store">Check out the iGEM store</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Luggage">What to do with your luggage</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Transportation">Transportation: How to get around</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Contact_Information">Contact Information</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#Avoid_the_Flu!">Avoid the Flu!</a></li><br />
</ul><br />
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<strong><a class="heading" href="https://2009.igem.org/Jamboree#Post-Jamboree">Post-Jamboree</a></strong><br />
<ul style="list-style:none;"><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#iGEM_2009_Results">Check out the results</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#iGEM_2009_press_.26_pictures">Share your pictures</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#iGEM_2009_press_.26_pictures">Share your press</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#iGEM_2009:_press_kit">iGEM 2009: press kit</a></li><br />
<li><a class="green" href="https://2009.igem.org/Jamboree#iGEM_2010">Get ready for iGEM 2010!</a></li><br />
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==Pre-Jamboree==<br />
====Jamboree Registration====<br />
Registration for the iGEM 2009 Jamboree is due on September 18th. A Jamboree attendance fee of $175.00 USD is required for each undergraduate or high school student and $375.00 USD for all instructors, grad students, advisors, and other attendees. Registration after September 18th will increase $50.00 and all payments must be received by October 2, 2009. Head to the [[Jamboree/Attendance_fee | attendance fee page]] to find out more details on how to register.<br />
<br />
====Project Abstract====<br />
In order to provide others with a summary of what your project is about, we will be asking each iGEM team to submit an abstract of 100-150 words by September 18th. Your abstract will be used in the Jamboree program that will provide a guide to the different topics that iGEM teams will be presenting. Please see the [[Jamboree/Project_abstract | project abstract]] page for more details.<br />
<br />
====Team Roster====<br />
In order to get a final list of team members, each team must finalize their team roster by September 18th. For your roster to be finalized, each team member must have a user account and be listed on the Team Information page. Note that all members must be confirmed and NOT listed as "pending" (i.e. they should be in the Team Roster section of the Team Information page. See [https://static.igem.org/mediawiki/2009/a/a5/Team_roster.png this example]. Be sure to include all members who participated over the summer, not only those who will be attending the Jamboree, as this list will be used to create participation certificates (so check for spelling mistakes too!). <br />
<br />
For more information on how to add members/edit your Team Information page see the [[Registration_Handbook | iGEM team Registration Handbook]].<br />
<br />
====Requests for Variance====<br />
One of the [[Requirements | requirements]] for iGEM is that teams are required to provide their parts to the Registry as standard biological parts in standard BioBrick plasmids. If a team wishes to use a different plasmid or different assembly method, they must document the new standard, explain their decision, and receive approval from iGEM Headquarters [[Calendar_of_Events | before September 18th]]. Parts in standards that are not approved by the judging committee will be judged as if no parts were submitted. See the [[Judging]] page for more details and examples. Please send your requests to judging AT igem DOT org.<br />
<br />
====Tracks====<br />
Track selection helps group similar projects together so that they may be judged fairly, share presentation time, and poster location. It is important that you submit your track selection on time, by September 18th. You can find a list of the tracks on the [[Jamboree/Tracks | tracks selection page]] and see [[Judging/Judging_Criteria#Area_Prizes | judging criteria]] for each track. Email your top 3 track selections, in order of preference, to hq AT igem DOT org.<br />
<br />
====Visa Letters====<br />
In iGEM 2008 there were several teams that were not able to attend the Jamboree due to visa problems. In order to avoid a similar problem this year, we asked that all teams apply for visas by June 1 at the latest. If you need to apply for a visa to come to the Jamboree, you can use our [[Jamboree/Visas | online form]] and print out an official invitation letter. <br />
<br />
iGEM Headquarters does not have any authority over consulate offices or the visa application process. The only way to have the highest chance of obtaining a visa is to apply early. If you do not yet know who will be attending the Jamboree in November, err on the side of caution and have all members apply for a visa. <br />
<br />
====Documentation====<br />
'''Project:''' According to the iGEM [[Requirements | requirements]], each iGEM team's project should be documented on the iGEM 2009 wiki site with detail enough to replicate it independently. Additional presentational information about the team - their story, the rationale for the project, failures, successes, future work, etc. - is highly encouraged. Remember that these wiki pages will be the main source of inspiration for future teams, and having good documentation on them and in the part description in the registry increases the likelihood of more teams building on your project and your parts. What is considered proper documentation is decided by the judging committee and you can find details about proper documentation at the [[Judging]] page.<br />
<br />
'''Parts:''' iGEM [[Requirements | requirements]] also state that all teams must properly document their parts on the [http://partsregistry.org Registry]. The judging committee also decides what is considered proper part documentation. See the [[Judging]] page for details. Remember, the success of not just iGEM, but all of synthetic biology, depends on the development of well-characterized, reliable, standardized biological parts that have been designed to be simple to use and understand.<br />
<br />
====DNA Submissions====<br />
iGEM teams are expected to submit their parts to the [http://partsregistry.org Registry of Standard Biological Parts]. In addition to submitting your parts, you must also declare which of you parts are you "favorites." Judges will only be looking at favorite parts so make sure to promote them before the October 21st deadline.<br />
<br />
See the [[DNA_Submission | DNA submission requirements]] for more information about the DNA submission process. <br />
<br />
====Poster Requirements====<br />
There will be two poster sessions at the iGEM 2009 Jamboree. Each team will be assigned to ONE of the two poster sessions. Locations will be assigned prior to your arrival at the Jamboree. You will be required to put up your poster by 9am on the day of your session. Posters shall be NO LARGER than 48 inches by 48 inches (1.22m x 1.22m).<br />
<br />
For details on poster judging see the [[Judging#Poster_judging | judging page]]. Keep in mind that the poster must be able to convey your team's project fully. It should be able to be judged without any external information or input from team members.<br />
<br />
iGEM HQ will be asking for a copy of your poster in pdf format for our files. You will be required to provide the pdf version of your poster at the time of your presentation.<br />
<br />
====Presentation Requirements====<br />
Each registered iGEM team is to make a 20-minute presentation, followed by 5 minutes of questions/answer session. A laptop will NOT be provided to your team -- please make sure you bring your own laptop from which to display your presentation as well as any necessary accessories to connect the laptop to a projector cable. <br />
<br />
iGEM HQ will also be asking for a copy of your presentation in pdf format for our files. You will be required to provide the pdf version of your presentation on Saturday, the day of Jamboree presentations. Please check back for more details on how to submit your presentation.<br />
<br />
====Software Tools Track====<br />
More details will be provided for the [[Software_tools_track | software tools track]] as the iGEM season progresses.<br />
<br />
====Experimental Track====<br />
The non-competition teams will be given the opportunity to present their work in a exposition part of the Jamboree. Teams will give 5-minute presentations, have poster space to present their posters, and compete for a special track specific award (they will not be eligible for the same awards as the traditional iGEM teams). Details about this track can be found on the [[Experimental track]] page.<br />
<br />
====Photo Release Form====<br />
Some of the products of the previous iGEM Jamborees are the iGEM from Above photographs. The use of these photographs, and all others from the Jamboree, was made possible due to the existence of a filled-out photo release form from every person attending the Jamboree. We must do the same for this year's Jamboree. In order to comply with the law, every person attending the Jamboree must fill out a photo release form. All participants attending the Jamboree must print, fill out, and bring a [[Jamboree/Release_form | photo release form]] with you to the Jamboree.<br />
<br />
====Hotels====<br />
iGEM does not provide travel or housing for any iGEM members. Participants are required to make their own travel and lodging arrangements. However, we have arranged for iGEM room blocks with special pricing at [[Jamboree/Hotels | nearby hotels]]. All iGEM Block rooms are available on a first-come, first-serve basis so it is recommended that you make your reservations early.<br />
<br />
====Guests====<br />
Guests, family, and friends are welcome to come to the Jamboree. Each must register and pay the [[Jamboree/Attendance fee | Jamboree attendance fee]]. There will be registration on site for last minute attendees but note that there is a $50 fee increase after September 18. <br />
<br />
As space is limited in presentation rooms and at the awards ceremony, preference will be given to iGEM team members. Extra seating will be available on a space available basis. Payment of the [[Jamboree/Attendance fee | Jamboree attendance fee]] does not guarantee a seat in presentation rooms or at the awards ceremony.<br />
<br />
====Volunteer====<br />
There will be many opportunities to volunteer at the Jamboree. We would especially like to encourage past iGEM participants (iGEM alums) to volunteer to help during the Jamboree. Head to the [[Jamboree/Volunteer | Volunteer]] page to find out more information.<br />
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<br />
==During the Jamboree==<br />
Welcome to the iGEM 2009 Jamboree! The next few days will be full of exciting presentations, stimulating conversations, well-deserved awards, and most of all, a lot of fun. The following information will guide you through the whole Jamboree event from what to expect at Friday night practice all the way up to the awards ceremony on Monday. Please read through this whole guide! It contains useful instructions and information that will ensure your Jamboree experience will go as smoothly as possible. And as a special note to [[Jamboree#Team_Leaders | team leaders]], you will have some extra responsibilities so please make sure you are aware of what you are expected to do.<br />
<br />
If you have a question at any point during the Jamboree, just look for one of the iGEM staff members in the black iGEM pullovers.<br />
<br />
Note: For all locations on the MIT campus – Stata Center, Lobby 13, Compton Lounge, and other event locations, consult the map in your registration packet.<br />
<br />
====Schedule====<br />
For the iGEM 2009 schedule, please see the [[Jamboree/Schedule | schedule page]].<br />
<br />
====Check-In====<br />
Teams can check-in Friday night (October 30) for the Jamboree beginning at 6pm. Check-in will take place at the Compton Lounge (Building 26). See the location on the map provided on the wiki and in your registration packet. <br />
<br />
At Check-in you will pick up your [[Jamboree#Team_Boxes | team box]] containing team member badges, participation certificates, registration packets, lunch tickets, and other important and useful information. Each [[Jamboree#Team_Leaders | team leader]] will be responsible for picking up the team box. This means that each member of the team DOES NOT have to stand in line at check-in. '''Important:''' in order to pick up your team box, you will be required to turn in all photo release forms for each person that is on your team roster. The release forms are absolutely necessary (see section on release forms below). Check-in personnel will check off each release form for each team member. Badge(s) pertaining to each person missing a release form will be held at Check-in until the form has been turned in. Badges will be necessary for entrance into presentation rooms, events, and for access to food. <br />
<br />
Guest Check-in begins Friday (October 30) at 6pm, also in the Compton Lounge. If you did not register online, it will be possible to register on-site at Compton Lounge during the course of the Jamboree. On Monday, however, on-site registration will take place at Kresge Auditorium. Registration costs $225 for undergraduates and high school students, and $425 for everyone else.<br />
<br />
====Badges====<br />
You will receive your name badge as part of your [[Jamboree#Team_Boxes | team box]], as long as you have submitted your photo release form. Please wear your badge at all times during the Jamboree and make sure it is clearly visible. Badges will be necessary for entrance into presentation rooms, for access to food, and for the iGEM social event. If you do not have a badge, you must register in order to obtain one. <br />
<br />
====Friday Night Practice====<br />
Teams will be allowed to practice on Friday night (October 30) in the Stata Center, beginning at 6:00pm. You can practice your presentation, and get to know fellow iGEM members. There are a limited number of rooms available, so please check [[Jamboree/Schedule/Practice sessions | availability online]]. We cannot match the practice room with the room you will give your presentation in on Saturday or Sunday. Note that there will not be technical staff on hand to help with audio/visual equipment. Please leave all presentation rooms in the condition that you found them. Friday night practice will run from 6:00pm to 10:00pm, and there will also be pizza and soda provided on a first come, first serve basis.<br />
<br />
Depending on your practice presentation room (32-261, 32-262, 32-346, 32-397), your team will need an iGEM staff member to escort you to your room as they are in a limited-access part of the Stata Center. Additionally, these rooms are small conference rooms. Saturday sessions will not be held in these rooms but in order to accommodate all teams who would like to practice their presentations in the 4-hour period on Friday night, we must open these rooms for practice sessions.<br />
<br />
Check the [[Jamboree/Schedule/Practice sessions | practice sessions page]] for availability.<br />
<span style="color:#D22325; font-weight:bold;">As the wiki has now been frozen, please email [[User:Meagan | Meagan]] with your team name and the letter and # of the slot that you would like to reserve.</span><br><br />
<br />
See details in the [[Jamboree#Posters | Posters section]] below on when you can set up your team poster. Note that this is different from previous years.<br />
<br />
====Photo Release====<br />
One of the products of the iGEM 2008 Jamboree was the [https://static.igem.org/mediawiki/2008/b/b0/Igem_from_above_cropped.jpg iGEM from Above photograph]. The use of this photograph, and all others from the 2008 Jamboree, was made possible due to the existence of a filled-out [[Jamboree/Release_form | photo release form]] from every person attending the Jamboree. We must do the same for the 2009 Jamboree. In order to comply with the law, every person attending the Jamboree must fill out a photo release form. There is a copy of the release form on the 2009 iGEM wiki that you should print, fill out, and bring with you to the Jamboree. '''Note:''' Team leaders, you must have a filled-out photo release form for each member of your team that is on the official roster before you can pick up your team member badges and team box. There will also be blank copies available at registration for you to fill out if you need another copy. If you have any questions or need further clarification, feel free to ask an iGEM staff member (in the black iGEM pullovers). <br />
<br />
Go to the [[Jamboree/Release_form | Release form page]] for an online and pdf version of the form. <br />
<br />
====Team Leaders====<br />
As mentioned above, team leaders have a few responsibilities. Each team leader (or a designated representative) will be responsible for gathering all team member photo release forms and handing them in at Check-in at Compton Lounge in order to obtain your team box. Team leaders are responsible for making sure individual team members receive the materials in the box. On Monday at Kresge Auditorium, team leaders will pickup their team medals after the ceremony. <br />
<br />
====Team Boxes==== <br />
Your team box will contain the following:<br />
<br />
*Team member badges<br />
*Map of iGEM events<br />
*Team member certificates<br />
*Jamboree Handbook<br />
*Program & Schedule<br />
*Team-Specific Information<br />
*Lunch Tickets<br />
*iGEM 2009 Social Event Info<br />
*Internet Connection Instructions<br />
*Sponsor Information<br />
*Other Information<br />
<br />
'''Notes:''' Team leaders are responsible for picking up team boxes and ensuring individual team members receive the necessary materials. Badges are to be worn at all times. <br />
<br />
====Posters==== <br />
Each team is required to present a poster at the Jamboree. There are two poster sessions, session ‘A’ on Saturday and session ‘B’ on Sunday, and your team will be assigned to ONE session. Furthermore, poster locations are assigned for each team in either the Stata Center or Lobby 13. Please see your poster information sheet, in your team box, for your team’s poster location.<br />
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The poster must be no larger than 4 ft (48 in, 121.92 cm) by 4 ft (48 in, 121.92cm). Each team may only put up ONE poster. The poster should be hung up on one of the poster stands that will be set up in the Stata Center, 1st and 4th floors, and Lobby 13, 1st floor. <br />
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Clear push pins, included in the team box, are to be affixed to each poster board. Please only use these clear push pins. Do not use tape or the grey pins along the edge of the board. The grey pins hold the poster board onto the frame, and if they are removed, the board will fall. Teams can setup their posters beginning at 8am on Saturday or Sunday depending on their session assignment.<br />
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Poster judges will be roaming throughout the day Saturday and Sunday. A green sticker affixed to your poster will indicate that it has been judged. The poster receptions will be held on Saturday and Sunday from 5pm and 7pm in the Stata Center and Lobby 13.<br />
<br />
Posters must be removed immediately following the poster receptions on both Saturday and Sunday, both of which end at 7pm. Please be sure to remove your poster at the end of the reception. Any remaining posters will not be saved.<br />
<br />
====iGEM around MIT campus====<br />
[[Jamboree/Campus_map | Campus map]]<br />
<br />
Due to the size of this year’s Jamboree, we have established two home bases on the MIT campus – the Stata Center and Lobby 13. The home bases, which are separated by project track, will serve as a central meeting point and hold team posters.<br />
<br />
Team presentations will take place in five rooms across the MIT campus: 32-123, 54-100, 10-250, 34-101 and 26-100. Please note that these presentation rooms are located across the MIT campus in five different buildings, but all rooms can easily be found off iGEM Alley. There will be maps and signs posted around each building, as well as the map provided to you, which will help direct you to the room you’re looking for. <br />
<br />
=====Stata Center is “Home Base” for:=====<br />
*Foundational Advance<br />
*Environment<br />
*Food & Energy<br />
*Software Tools<br />
<br />
'''Associated Rooms:''' 32-123, 54-100, 34-101<br />
<br />
=====Lobby 13 is “Home Base” for:=====<br />
*Health & Medicine<br />
*Manufacturing<br />
*New Application<br />
*Information Processing<br />
<br />
'''Associated Rooms:''' 10-250, 26-100, 34-101<br />
<br />
====Presentations====<br />
In each room, the presentations will be divided into four sessions with each session comprised of three separate presentations. Each of the three presentations given during a session will be of the same ‘track’. Please be sure to bring the necessary equipment for your presentation, such as your laptop, cables/adaptors, and power supply, as iGEM will not provide these. If you are attending the presentations, please stay for the whole session and only leave the room during the scheduled coffee breaks. The schedule of the presentations can be found in your registration packet and on the online [[Jamboree/Schedule | schedule page]]. <br />
<br />
====We need your posters & presentations!==== <br />
In an effort to capture all of the hard work that teams have put into their iGEM projects, we ask that each team give us a copy of your presentation and a copy of your poster. <br />
<br />
To submit your files follow the instructions below. <br />
<br />
*Save your presentation and poster as a pdf file. <br />
*Ten minutes before the start of each session, there will be an iGEM staff member at the front of each presentation room. <br />
*Bring your laptop with the files on it to the front and the iGEM staff member will transfer your presentation and poster to a USB key that they will have with them. <br />
<br />
Please make sure to do this in the 10 minutes prior to the start of the session! (NOT prior to your presentation time) <br />
<br />
Consult the [[Jamboree/Schedule | schedule ]], online or in your registration packet, to see which session you are presenting in. <br />
<br />
====iGEM from Above====<br />
After the finalists have given their presentations on Monday, we will be taking the 2009 iGEM from Above picture! The picture will be taken at Killian Court, a short walk from Kresge Auditorium. In the unfortunate event of bad weather, the picture will be taken at Kresge. Make sure to wear your iGEM [[Jamboree/Team_Spirit | team t-shirt]]!<br />
<br />
====Medals====<br />
This year we are once again designating achievements for documentation of your project and contributions to the synthetic biology community. These achievements are signified by a bronze, silver, or gold medal seal, which each team member can adhere to their participatory certificate. These designations will be based on their performance in the online judging round (online judging occurs in the week directly before the Jamboree).<br />
<br />
Check out the [[Judging/Judging_Criteria | Judging Criteria]] page for the requirements for each medal level.<br />
<br />
Team Leaders can pick up their team medals (one for each member on the official roster) on Monday afternoon after the awards ceremony in the lobby of Kresge Auditorium. It is intended that your medal seal be placed on your participation certificate (for each team member). Frames for participation certificates can also be picked up in the lobby after the awards ceremony.<br />
<br />
====Awards/Frames====<br />
[[Judging/Awards | Awards]] will be presented at the awards ceremony on Monday (November 2). Each team that wins an award will receive one trophy for the team as well as award certificates for each team member. These award certificates are separate from the participation certificates that all iGEM teams receive from their team box. Both the award certificates and the participation certificates look great when framed. Certificate frames will be provided by iGEM HQ and will be available in the lobby of Kresge Auditorium after the awards ceremony finishes. Boxes for the awards will also be provided at this time.<br />
<br />
====iGEM Wrap-up====<br />
After the award ceremony on Monday (November 2) we will be hosting a discussion session on iGEM 2009 and the future of iGEM with iGEM 2010 and beyond. We will not be providing lunch during this time, so you are free to eat out and return for the discussion which will begin approximately 1 hour after the end of the awards ceremony.<br />
<br />
Lunch Suggestions<br />
At the Stratton Student Center (W20), a short walk from Kresge Auditorium, there are a number of eateries: <br />
<br />
Cambridge Grill<br />
Anna's Taqueria,<br />
Cafe Spice (2nd Floor)<br />
Sepal (2nd Floor)<br />
Shinkansen Japan (2nd Floor)<br />
Subway (2nd Floor)<br />
and others…<br />
<br />
Further away, but right by the Kendall/MIT train station, there are several food trucks serving a variety of foods on Carleton St.<br />
<br />
For more detailed information, please see http://dining.mit.edu, which provides a food map, short descriptions, menus and operating hours.<br />
<br />
====Family and Friends==== <br />
Family and friends are welcome to [[Jamboree/Attendance_Fee | register]] for all iGEM Jamboree events. If non-iGEM team members (e.g. family, friends, guests) want to attend the presentation sessions, poster sessions, social on Sunday, and the awards ceremony, they have to officially register and get a badge (on-site registration is available and can be paid for with all major credit cards, US$425). Entrance to the awards ceremony is not guaranteed, and is dependent on the capacity of Kresge Auditorium. <br />
<br />
====Food====<br />
Food will be provided throughout the Jamboree. Badges must be (visibly) worn in order to have access to all food. <br />
<br />
Teams have been randomly assigned to lunch locations in order to spread out the crowd and cut down on the time you have to wait on line. We ask that you please pick up your lunch in your assigned location, although you are free to eat it where you wish. <br />
<br />
Note that your team boxes will contain color-coded lunch tickets that you will need to present in order to obtain your lunch. See the flyer in your registration folder for the menu and your lunch location.<br />
<br />
====Social Event: Sunday night====<br />
On Sunday night after the poster reception, we will be hosting an iGEM Jamboree social event at [http://www.jilliansboston.com/ Jillian’s of Boston] from 8:00pm to 12:00am. There will be food, dancing, billiards, bowling, lounge space on the 1st and 2nd floors of Jillian's. Each person will receive two drink tickets at the door for soft drinks/soda. For those who are 21 and over there will be a cash bar. The drinking age in the US is 21 so please keep this in mind. Bartenders will be checking IDs so please have your US ID or passport with you as identification. We have arranged to have shuttle buses to bring people from the MIT campus and the Hyatt to the event location at Jillian's in the Fenway area of Boston. See the map in your registration packet for shuttle stop locations. <br />
<br />
'''Note:''' You must have your iGEM name badge in order to gain access to the social event.<br />
<br />
====Compton Lounge====<br />
Compton Lounge (Building 26) will house the pre-registration/check-in on Friday starting at 6:00pm. On-site registration will also be available and costs $225 for undergraduates and high school students, and $425 for everyone else.<br />
<br />
The Information Center will be located at Compton Lounge as well; feel free to ask any questions you may have. We will also have iGEM Staff available for assistance at all of the event locations.<br />
<br />
Lastly, if you want some great iGEM gear, you can also find the iGEM Store at Compton Lounge. We will be selling iGEM hoodies (sweatshirts) and patches.<br />
<br />
====iGEM Store====<br />
Come buy some iGEM products at the iGEM store! This year we will be selling [https://static.igem.org/mediawiki/2008/a/a7/Sweatshirts.JPG iGEM hoodies] (sweatshirts) for US$30 and iGEM iron-on patches for US$5. Wear your hoodies on those chilly days to keep you warm and stick your patch on your backpack, lab coat, jacket, or any other place you want to express your love for iGEM! And don't forget to buy some for your family and friends. The iGEM store will accept US dollars and all major credit cards. <br />
<br />
====Luggage====<br />
If you need to check out of your hotel on Monday morning and need to stow your luggage somewhere, you can bring it to Kresge Auditorium. However, we cannot guarantee that it will be locked or supervised during the awards ceremony. If you do bring your luggage to Kresge Auditorium it is absolutely critical that your luggage be picked up by 2:30 pm. <br />
<br />
====Transportation====<br />
The cities of Boston and Cambridge have a public transportation system that is comprised of buses and subways. You can find more information for the MBTA here: http://www.mbta.com. The Jamboree and its event locations are located by the MBTA Red Line at the Kendall/MIT subway stop. There are also several bus stops in the area.<br />
<br />
The MIT shuttle has stops across the campus. <br />
<br />
See [http://web.mit.edu/facilities/transportation/shuttles/index.html MIT's Shuttle website] for more information. <br />
<br />
====Contact Information====<br />
If you need to get in touch with anyone at iGEM headquarters (HQ) for an urgent matter, you may contact the following people:<br />
<br />
*Randy Rettberg<br />
*[[User:Meagan | Meagan Lizarazo]]<br />
<br />
====Emergency Information====<br />
If there is an emergency (medical emergency, fire, police, etc.) please contact MIT campus emergencies numbers:<br />
<br />
*From a campus phone: 100<br />
*From a cell phone, pay phone, or off-campus: +1-617-253-1212<br />
<br />
====Avoid the Flu!====<br />
Influenza-related illness often peaks during the cold season in the New England area. It’s always important to take these simple precautions to protect yourself and others:<br />
*Wash your hands frequently and thoroughly with soap and warm water, or use an alcohol-based hand sanitizer<br />
*Cover coughs and sneezes<br />
*Avoid touching your eyes, nose, and mouth<br />
*Stay home if you are feeling sick<br />
<br />
=====When should I seek medical attention?===== <br />
You may need to see a care provider if you have the following serious flu symptoms:<br />
*Fever higher than 101 degrees for two to three days<br />
*Nausea or vomiting and an inability to keep fluids down<br />
*Significant respiratory symptoms, including a big cough, wheezing, or shortness of breath<br />
*Yellow or green discharge from the nose or produced with a cough<br />
*Severe headache, especially with stiff neck; ear or facial pain, persistent sore throat; white spots on tonsils; and/or unusual rashes are not typically associated with influenza, but these symptoms may indicate other serious illnesses for which you should seek medical attention.<br />
<br />
<br />
----<br />
<br />
<br />
==Post-Jamboree==<br />
====iGEM 2009 Results====<br />
iGEM 2009 results will be announced at the Jamboree awards ceremony on Monday November 2nd. Please check back for results then.<br />
<br />
====iGEM 2009 press & pictures====<br />
If you have any online articles about your team or iGEM that you would like to share, please post them on the [[IGEM_Publicity| Publicity page]].<br />
<br />
If you have pictures that you would like to share with us, post a link to them on the our Media page.<br />
<br />
====iGEM 2009: press kit====<br />
For the iGEM boilerplate, high-resolution logos, and more material for the press, please see the [[Press_Kit | press kit]] page.<br />
<br />
====iGEM 2010====<br />
We will be posting information about iGEM 2010 soon after the Jamboree so make sure you check back if you are interested in participating!<br />
</div></div>Meaganhttp://2009.igem.org/File:Igem_from_above_2009_logo_400px.jpgFile:Igem from above 2009 logo 400px.jpg2010-09-28T17:36:14Z<p>Meagan: </p>
<hr />
<div></div>Meaganhttp://2009.igem.org/Job_OpportunitiesJob Opportunities2010-01-13T18:54:23Z<p>Meagan: </p>
<hr />
<div>'''Please see the [https://2010.igem.org/Job_Opportunities Job Opportunities page] on [https://2010.igem.org 2010.igem.org] for more information about job opportunities at iGEM Headquarters.'''</div>Meaganhttp://2009.igem.org/Main_PageMain Page2010-01-13T18:53:07Z<p>Meagan: </p>
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title="Repository freezer plates."><br />
<img src="https://static.igem.org/mediawiki/2009/3/3d/Slovenia_mascot_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
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<img src="https://static.igem.org/mediawiki/2009/5/57/Igem_banner_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: iGEM banner." <br />
title="iGEM banner."> <br />
<!-- <img src="https://static.igem.org/mediawiki/2009/3/37/Finalist_announce_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: 2008 finalist announcement"<br />
title="Announcing the finalists at the 2008 Jamboree."> --><br />
<!-- <img src="https://static.igem.org/mediawiki/2009/2/23/Dhl_boxes_2_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: DHL boxes"<br />
title="DHL boxes for the DNA distribution."> --><br />
<img src="https://static.igem.org/mediawiki/2009/3/38/Blackboard_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: Tagging blackboard!"<br />
title="Tagging the iGEM blackboard!"><br />
<img src="https://static.igem.org/mediawiki/2009/3/3a/Phage_mascot_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
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<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> <a class="quick_link" href="https://2009.igem.org/Jamboree">iGEM 2009 Jamboree</a></h3><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center; font-weight:bold;">October 31 to November 2, 2009</p><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;">Massachusetts Institute of Technology</p><br />
<ul style="list-style:none;" ><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Quick links:</lh><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Project_Abstract/Team_Abstracts">Team abstracts</a></li><br />
<li><a class="quick_link" href="https://igem.org/Team_Wikis?year=2009">Team websites</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Schedule">Schedule</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Campus_map">Campus Map</a></li><br />
<li><a class="quick_link" href="https://igem.org/Results?year=2009">iGEM 2009 Jamboree results</li><br />
</ul><br />
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<img src="https://static.igem.org/mediawiki/2009/9/95/2009_igem_from_above_300.jpg"<br />
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<!-- NEWS --><br />
<p style="padding: 10px; margin: 10px; color: #666; font-family: Lucida Grande, Verdana, Arial, sans-serif;"><span style="font-size:115%; font-weight:bold; color:#576f91;">News:</span> Check out the <a href="https://2010.igem.org/Job_Opportunities">new job opportunities</a> at iGEM Headquarters.</p><br />
<br />
<br />
<br />
<!-- ADD YOUR PUBLICITY --><br />
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<p style="font-size:130%; /*font-weight:bold;*/ color:#576f91;">Add your iGEM 2009 <a class="green_link" href="https://2009.igem.org/IGEM_Publicity">publicity</a>, <a class="green_link" href="https://2009.igem.org/Photo_Gallery">photos</a>, & <a class="green_link" href="https://2009.igem.org/IGEM_Publications">publications</a></p><br />
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<!-- *** JAMBOREE RESULTS *** --><br />
<div id="jamboree_results_box" style="width:700px; margin:0px 0px 30px 0px;"><br />
<p style="font-size: 200%; font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#ed0100; text-align:center; margin-left:20px;">iGEM 2009 is officially over!</p><br />
<img src="https://static.igem.org/mediawiki/2009/2/2a/Jamboree2009_cambridge_award.jpg"<br />
style="margin-left:125px; border:2px solid #aaa"><br />
<br />
<!-- GRAND PRIZE, 1ST, 2ND --><br />
<ul style="list-style:none; font-size:110%; color:#666; font-family: Lucida Grande, Verdana, Arial, sans-serif; text-align:center;"><br />
<li><strong>Grand Prize, Winner of the BioBrick Trophy:</strong> Cambridge</li><br />
<li><strong>1st Runner Up:</strong> Heidelberg</li><br />
<li><strong>2nd Runner Up:</strong> Valencia</li><br />
</ul><br />
<br />
<!-- FINALISTS --><br />
<div id="finalist_list"><br />
<ul style="list-style: none;"><br />
<lh>Finalists:</lh><br />
<li>Cambridge</li><br />
<li>Freiburg bioware</li><br />
<li>Groningen</li><br />
<li>Heidelberg</li><br />
<li>Imperial College London</li><br />
<li>Valencia</li><br />
</ul><br />
</div> <!-- close finalists --><br />
<br />
<!-- TRACK AND SPECIAL AWARDS --><br />
<table style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666;"><br />
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<ul style="list-style:none;"><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Track Award Winners:</lh><br />
<li><strong>Best Food or Energy Project:</strong> UNIPV-Pavia</li><br />
<li><strong>Best Environment Project:</strong> Cambridge</li><br />
<li><strong>Best Health or Medicine Project:</strong> Stanford</li><br />
<li><strong>Best Manufacturing Project:</strong> Imperial College London</li><br />
<li><strong>Best New Application Area:</strong> Valencia</li><br />
<li><strong>Best Foundation Advance:</strong> Alberta</li><br />
<li><strong>Best Information Processing Project:</strong> TUDelft</li><br />
<li><strong>Best Software Tool:</strong> Berkeley Software & Illinois-Tools <small>(Tie)</small></li><br />
</ul><br />
</td><br />
<td style="width:350px;"><br />
<ul style="list-style:none;"><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Special Prizes Winners:</lh><br />
<li><strong>Best BioBrick Part, Natural:</strong> ULB-Brussels</li><br />
<li><strong>Best New BioBrick Part or Device, Engineered:</strong> EPF-Lausanne & Freiburg Bioware <small>(Tie)</small></li><br />
<li><strong>Best Human Practices Advance:</strong> Imperial College London & Paris <small>(Tie)</small></li><br />
<li><strong>Best Experimental Measurement:</strong> Valencia</li><br />
<li><strong>Best Model:</strong> BCCS-Bristol</li><br />
<li><strong>Best Wiki:</strong> Heidelberg</li><br />
<li><strong>Best Poster:</strong> Freiburg bioware</li><br />
<li><strong>Best Presentation:</strong> ArtScienceBangalore</li><br />
<li><strong>Best New Standard:</strong> Heidelberg</li><br />
</td><br />
</tr><br />
</table><br />
<p style="font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> See the <a href="https://igem.org/Results?year=2009">Results page</a> for details</p><br />
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</html></div>Meaganhttp://2009.igem.org/File:2009_igem_from_above_175.jpgFile:2009 igem from above 175.jpg2010-01-13T17:59:13Z<p>Meagan: </p>
<hr />
<div></div>Meaganhttp://2009.igem.org/File:Get_logo_here_100h.pngFile:Get logo here 100h.png2010-01-13T17:56:04Z<p>Meagan: </p>
<hr />
<div></div>Meaganhttp://2009.igem.org/File:Get_logo_here_50h.pngFile:Get logo here 50h.png2010-01-13T17:53:52Z<p>Meagan: </p>
<hr />
<div></div>Meaganhttp://2009.igem.org/File:Get_logo_here_small.pngFile:Get logo here small.png2010-01-12T16:35:47Z<p>Meagan: </p>
<hr />
<div></div>Meaganhttp://2009.igem.org/SecuritySecurity2010-01-11T19:14:18Z<p>Meagan: </p>
<hr />
<div><span style="color:#ab0000; font-weight:bold;">** Note: For the most up-to-date information please see the [https://2010.igem.org/Security Security page] on the [https://2010.igem.org iGEM 2010 wiki]. **</span><br />
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<p align=center><b>Resources</b></p><br />
<br />
<p><b>People</b></p><br />
<br />
<img src="https://static.igem.org/mediawiki/2009/4/46/Piers1_60per.jpg"><br />
<ul><br />
<li><B>Piers Millet</b></li><br />
<li>BWC ISU</li><br />
<li><a href="mailto:bwc@unog.ch">bwc@unog.ch</a></li><br />
<li><a href="http://www.unog.ch/bwc">www.unog.ch/bwc</a></li><br />
</ul><br />
<p>The BWC ISU is the closest thing to an international organisation to ensure biology is used solely for beneficial purposes. It is housed in the UN Office for Disarmament Affairs in Geneva and, as Deputy Head, Piers helps States Parties to the Biological Weapons Convention ban the hostile use of biology. As a microbiologist and chartered biologist, Piers supports the technical aspects of the ISU's work.</p><br />
<br><hr><br />
<br />
<p><b>Reports</b></p><br />
<br />
<a target="_blank" href="http://www.synbiosafe.eu/uploads///pdf/Synthetic%20Genomics%20Options%20for%20Governance.pdf"><br />
<img src="https://static.igem.org/mediawiki/2009/4/4a/SB-Venter_CSIS_MIT.JPG"></a><br />
<b>Synthetic Genomics: Options for Governance</b> by the J Craig Venter Institute, CSIS and MIT, October 2007<br />
<br><br><br><br><br />
<br />
<a target="_blank" href="http://www.synbiosafe.eu/uploads///pdf/Synbiosafe-Biosecurity_awareness_in_Europe_Kelle.pdf"><br />
<img src="https://static.igem.org/mediawiki/2009/a/ab/SB-Biosec_Awareness_in_Europe.JPG"></a><br />
<b>Synthetic Biology and Biosecurity Awareness in Europe</b> by SynBioSafe, November 2007<br />
<br><br><br><br><br />
<br />
<a target="_blank" href="http://www.bbsrc.ac.uk/organisation/policies/reviews/scientific_areas/0806_synthetic_biology.pdf"><br />
<img src="https://static.igem.org/mediawiki/2009/f/fd/SB-Soc_%26_eth.JPG"></a><br />
<b>Synthetic Biology: Social and Ethical Challanges</b> by the Institute for Science and Society, May 2008<br />
<br><br><br><br><br />
<br />
<a target="_blank" href="http://www.synbioproject.org/process/assets/files/5999/synbio1final.pdf?"><br />
<img src="https://static.igem.org/mediawiki/2009/a/ab/SB-US_%26_European_Press_Coverage.JPG"></a><br />
<b>Trends in American and European Coverage of Synthetic Biology</b> by the Woodrow Wilson International Center for Scolars, 1 November 2008<br />
<br><br><br><br />
<br />
<a target="_blank" href="http://www.synbiosafe.eu/uploads///pdf/iasb_report_biosecurity_syntheticbiology.pdf"><br />
<img src="https://static.igem.org/mediawiki/2009/3/32/SB-IASB.JPG"></a><br />
<b>Technical solutions for biosecurity in synthetic biology</b> by the Industry Association Synthetic Biology, 2008<br />
<br><br><br><br><br />
<br />
<a target="_blank" href="http://www.raeng.org.uk/news/publications/list/reports/Synthetic_biology.pdf"><br />
<img src="https://static.igem.org/mediawiki/2009/5/5a/RAE-SB.JPG"></a><br />
<b>Synthetic Biology: Scope Applications and Implications</b> by the UK Royal Academy of Engineering, May 2009<br />
<br><br><br><br><hr><br />
<br />
<p><b>Other Resources</b></p><br />
<br />
<b>Why Secure Modern Biology?</B><br />
<center><br />
<object width="250" height="152"><param name="movie" value="http://www.youtube.com/v/hcyxWnhWsAQ&hl=en&fs=1&"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="http://www.youtube.com/v/hcyxWnhWsAQ&hl=en&fs=1&" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="250" height="152"></embed></object><br />
</center><br />
<p> This is a 30 minute video arguing why we need to secure synthetic biology. It was filmed at SB4.0 in Hong Kong in 2008. It includes a short quiz that demonstrates how hard it is to spot the use of biology for hostile purposes. It looks at some of the problems with trying to secure biology through top-down governmental approaches and the need to find a community-based response to this shared problem.</p><br />
<br />
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<div id="security_information"><br />
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<td width=115 align=center><img src="https://static.igem.org/mediawiki/2009/3/3f/Secuity_-_naughty_or_nice-_small.jpg"></td><br />
<td width=135 align=center><img src="https://static.igem.org/mediawiki/2009/a/a3/Video_cameras-small.jpg"></td><br />
<td width=155 align=center><img src="https://static.igem.org/mediawiki/2009/6/67/Bio_wordle.jpg"></td><br />
<td width=135 align=center><img src="https://static.igem.org/mediawiki/2009/b/bd/Zero-small.jpg"></td><br />
<td width=135 align=center><img src="https://static.igem.org/mediawiki/2009/b/b3/Security_fence_-_small.jpg"></td><br />
</tr><br />
</table><br />
<br />
<table width=675><br />
<tr><td style='padding-left:20px;padding-right:20px;'> <br />
<b>"Biology should be more fun. It should be about exploring the world around us. We should want to get out there and do things. We should be able to do things more easily. Securing biology should be something that helps us do that. It cannot be something that gets in the way."</b> <br />
</td></tr><br />
</table><br />
<br />
<p>Scientific research continues to bring us new and unexpected knowledge, technologies and approaches. Synthetic biology, being on the very cutting edge of what is possible, promises unprecedented opportunities for health, wealth and better living. But science and technology can be used for destructive purposes as well as for constructive ones. Refining our control of biology opens up chances to intentionally cause harm to humans, animals, plants and the environment that just did not exist before. That’s why it is important now, more than ever, for us to think about how others might use what we are doing in ways we would not be happy with.</p><br />
<br />
<hr><br />
</div><br />
<br />
<br />
<div id="preventing_malign_use"><br />
<br />
<table border="0"><br />
<tr><br />
<td valign=Top><br />
<b>Preventing Malign Use </b><br />
<br><br><br />
Securing biology is not a simple task. It is not something those outside biology could, or should, do alone. Equally, this is not something that biologists can do by themselves (our focus, as the name implies is on the biology). This is a truly interdisciplinary problem - one that means we will need to work together, in new ways, with new partners, to find an approach that provides benefits for all. Given the interdisciplinary nature of synthetic biology, and the experience the community has in drawing in people from different backgrounds, we are well placed to position ourselves in the vanguard of those thinking about how science interacts with society and to help write the rules which will govern this ‘century of biology’. <br />
</td><br />
<br />
<td width=5%><br />
</td><br />
<br />
<td width=45% valign=Top bgcolor="#B2D0B4"> <br />
<br><br />
As a participant in iGEM, there are three things you can do right now to help us secure our science:<br />
<br><br><br />
<ol><br />
<li>Include something in your project description and presentations that demonstrates that you have thought about how others could misuse your work</li><br />
<li>Contribute to community discussions on what needs to go into a code against the use of our science for hostile purposes (see A Community Response)</li><br />
<li>Look into what security provisions, such as laws and regulations, are already in place in your country (see Working within the Law)</li><br />
</ol><br />
</td><br />
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<br />
<br />
<div id="shaping_the_future"><br />
<b>Shaping the Future</b><br />
<br><br><br />
<p>Ensuring that biology is used safely, securely and constructively should be of concern to us all. This is a challenge we will have to face together. To do this we will need to figure out what we want biological engineering to look like, what we are prepared for others to do with it, and just how we want to tackle security issues. This page provides a space to focus on these issues and for you to help shape what should be done to stop those with a malign intent. There is a real opportunity here for iGEM and those participating in iGEM, not only to shape how they will deal with security issues but to drive their national and even international processes. You can make a real difference in securing biology – in your lab, in your country and across the world.</p><br />
<hr><br />
</div><br />
<br />
<br />
<div id="do_no_harm"><br />
<b>A Committment to Do No harm</b><br />
<br><br><br />
<p>There is a strong feeling amongst those involved with iGEM, as well as the broader synthetic biology community, that the work we do should be used only for the benefit of humankind. It should not be used to do harm or to make weapons. This understanding has prompted some to think about what the community can do to ensure that this never happens. One important step would be a code or personal declaration that everyone involved (from the organisers, through supervisors and advisors, to team members and even the mascots!) would commit themselves to. This helps to ensure that we think about security as something that does directly involve us, is part of our project and can be dealt with in a way that helps us to get on and have some fun engineering biology.</p><br />
<hr><br />
</div><br />
<br />
<br />
<div id="community_response"><br />
<b>A Community Response</b><br />
<br><br><br />
<br />
<table border="0"><br />
<tr><br />
<td width=55% valign=Top bgcolor="#B2D0B4"> <br />
<b>Areas a code might address:</b><br />
<ul><br />
<li>An <b>introduction</b> about why there is a code</li><br />
<li>A reminder of the importance of <b>personal responsibility</b> and that as your career progresses so do your responsibilities</li><br />
<li>A commitment to <b>get informed</b> about principles and practices designed to prevent hostile use</li><br />
<li>A commitment to find out about and <b>comply with regulatory frameworks</b>, such as international, national and institutional laws and guidelines</li><br />
<li>An obligation to <b>do no harm</b></li><br />
<li>A requirement to look at the <b>reasonably foreseeable consequences</b> of your activities</li><br />
<li>A commitment <b>not to ignore possible breaches</b> of the code by others</li><br />
<li>An obligation to <b>act responsibly</b> in case you stumble across something that does not easily sit with the aims of the code</li><br />
<li>Some link between the pursuit of <b>science and the best interests of the society</b> in which it is pursued </li><br />
</ul><br />
</td><br />
<br />
<td width=5%><br />
</td><br />
<br />
<td valign=Top><br />
Any code that would commit community members to do no harm would be first and foremost the property of this community. There is a solid body of work dedicated to the sorts of things that might be included in such an effort (see the Resources section). But nothing should be taken for granted – the content of our code would be up to us to decide. Here are some of the areas that a code might cover. Are these accurate? Can you think of anything else that should be on this list? Is there too much and we should get rid of some (if so, what)? Is there any point to working on a code? Here is where you can get involved – we are hoping that you will have something to say. You can answer these questions or add anything else you want to say (you know the drill) in the comments section below. <br />
</td><br />
</tr><br />
</table><br />
<br />
<br><br />
<hr><br />
</div><br />
<br />
<br />
<div id="working_withi_the_law"><br />
<b>Working Within the Law</b><br />
<br><br><br />
<p>There is an international treaty that prohibits the use of biology for hostile or malign purposes. If you use biology to do harm you will be breaking international law.</p><br />
<br><br />
<p>Many countries also have their own laws about using biology in this way. They are increasingly backed up with regulations and guidelines that are relevant to the day to day functioning of a laboratory. It is important that we are all familiar with the rules that cover our work. Whilst we are commonly taught how we should work safely, we are less often taught how to work securely.</p><br />
<br><br />
<p>This section provides a gateway to details of some of these national regulatory frameworks. We hoping that you will use this to make sure you know all you need to know about staying out of trouble. But we are hoping that you will also be able to help us improve this resource. We have provided some information on some of the measures in some of the countries with the largest participation in iGEM. We know this is not a list of all relevant measures in all countries that participate. Here is where you come in. Is there something missing you know applies in your country? If so why not add some information? If you don't know what is in place, why not find out and let us know? That would really help future teams, your professional conduct and the community as a whole.</p><br />
<br><br />
<br />
<i>China</i><br />
<ul><br />
<li><a target="_blank" href="http://www.unog.ch/80256EDD006B8954/(httpAssets)/BBCCCC514AA386A3C1257355003AA13D/$file/BWC_NID_Report-070912.htm#chi">Laws in China relevant to work with biological agents</a></li><br />
</ul><br />
<br><br />
<br />
<i>Switzerland</i><br />
<ul><br />
<li><a target="_blank" href="http://www.unog.ch/80256EDD006B8954/(httpAssets)/BBCCCC514AA386A3C1257355003AA13D/$file/BWC_NID_Report-070912.htm#swi">Laws in Switzerland relevant to work with biological agents</a></li><br />
<li><a target="_blank" href="http://www.unog.ch/unog/website/disarmament.nsf/(httpPages)/FD59A71FC0B3FAF8C12574780052F81A?OpenDocument">Swiss arrangements for biosafety and biosecurity</a></li><br />
<li><a target="_blank" href="http://www.unog.ch/unog/website/disarmament.nsf/(httpPages)/6175389B1B34CBA6C125747800540E12?OpenDocument">Swiss arrangements for the oversight of science</a></li><br />
</ul><br />
<br><br />
<br />
<i>United Kingdom</i><br />
<ul><br />
<li><a target="_blank" href="http://www.unog.ch/80256EDD006B8954/(httpAssets)/BBCCCC514AA386A3C1257355003AA13D/$file/BWC_NID_Report-070912.htm#uk">Laws in the UK relevant to work with biological agents</a></li><br />
<li><a target="_blank" href="http://www.unog.ch/unog/website/disarmament.nsf/(httpPages)/FD59A71FC0B3FAF8C12574780052F81A?OpenDocument">UK arrangements for biosafety and biosecurity</a></li><br />
</ul><br />
<br><br />
<br />
<i>United States</i><br />
<ul><br />
<li><a target="_blank" href="http://www.unog.ch/80256EDD006B8954/(httpAssets)/BBCCCC514AA386A3C1257355003AA13D/$file/BWC_NID_Report-070912.htm#us">Laws in the US relevant to work with biological agents</a></li><br />
<li><a target="_blank" href="http://www.unog.ch/unog/website/disarmament.nsf/(httpPages)/FD59A71FC0B3FAF8C12574780052F81A?OpenDocument">US arrangements for biosafety and biosecurity</a></li><br />
<li><a target="_blank" href="http://www.unog.ch/unog/website/disarmament.nsf/(httpPages)/6175389B1B34CBA6C125747800540E12?OpenDocument">US arrangements for the oversight of science</a></li><br />
</ul><br />
<br><br />
<br />
<hr><br />
</div><br />
<br />
<br />
<div id="got_questions"><br />
<b>Got Questions?</b><br />
<br><br><br />
<p>If there is anything here that has caught your interest, infuriated you and sparked any other reason you would like to get in touch, then please do. You can leave comments, thoughts and suggestions below but also feel free to contact us directly if you want something a little more interactive. </p><br />
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<html> <br />
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{{Security_Comments}}<br />
</div></div>Meaganhttp://2009.igem.org/Press_KitPress Kit2010-01-11T17:25:46Z<p>Meagan: Undo revision 169691 by Meagan (Talk)</p>
<hr />
<div>===iGEM Boilerplate===<br />
The International Genetically Engineered Machine competition (iGEM) is the premiere undergraduate Synthetic Biology competition. Student teams are given a kit of biological parts at the beginning of the summer from the Registry of Standard Biological Parts. Working at their own schools over the summer, they use these parts and new parts of their own design to build biological systems and operate them in living cells. This project design and competition format is an exceptionally motivating and effective teaching method. <br />
<br />
iGEM began in January of 2003 with a month-long course during MIT's Independent Activities Period (IAP). The students designed biological systems to make cells blink. This design course grew to a summer competition with 5 teams in 2004, 13 teams in 2005 - the first year that the competition grew internationally, 32 teams in 2006, 54 teams in 2007, and 84 teams in 2008. Projects ranged from banana and wintergreen smelling bacteria, to an arsenic biosensor, to Bactoblood, and buoyant bacteria. <br />
<br />
This year, we have 112 teams with over 1700 participants from countries across Asia, Europe, Latin America, and the US participating in the competition. They will specify, design, build, and test simple biological systems made from standard, interchangeable biological parts. The accomplishments of these student teams during one summer are often impressive and may lead to important advances in medicine, energy, and the environment. Teams will present their projects at the iGEM Championship Jamboree October 30 to November 2, 2009.<br />
<br />
For more information please visit [http://www.igem.org www.igem.org].<br />
<br />
===iGEM Logo===<br />
Download the iGEM logo '''[https://static.igem.org/mediawiki/2008/4/44/Igem_logos.zip here]'''.<br />
Note that the iGEM logo is copyrighted by iGEM and provided here for iGEM participants to help them promote iGEM and their iGEM team.<br />
<br />
<br />
<br />
===iGEM Contact Information===<br />
Please direct all press questions to:<br />
<html><br />
<div style="float:left; padding:20px;"><br />
<a href="https://2008.igem.org/Contact"><br />
<img src="https://static.igem.org/mediawiki/2008/2/21/Randy_contact_small.png"<br />
alt="Randy AT igem DOT org"<br />
title="Randy AT igem DOT org"><br />
</a><br><br />
<strong>Randy Rettberg</strong>, <em>Director</em><br><br />
+1.617.258.5244<br />
</div><br />
<br />
<div style="float:left; padding:20px;"><br />
<a href="https://2008.igem.org/User:Meagan"><br />
<img src="https://static.igem.org/mediawiki/2008/5/5e/Meagan_contact_small.png"<br />
alt="Meagan AT igem DOT org"<br />
title="Meagan AT igem DOT org"><br />
</a><br><br />
<strong>Meagan Lizarazo</strong>, <em>Assistant Director</em><br><br />
+1.617.258.5244<br />
</div><br />
</html></div>Meaganhttp://2009.igem.org/Press_KitPress Kit2010-01-11T17:25:10Z<p>Meagan: </p>
<hr />
<div>===iGEM Boilerplate===<br />
The International Genetically Engineered Machine competition (iGEM) is the premiere undergraduate Synthetic Biology competition. Student teams are given a kit of biological parts at the beginning of the summer from the Registry of Standard Biological Parts. Working at their own schools over the summer, they use these parts and new parts of their own design to build biological systems and operate them in living cells. This project design and competition format is an exceptionally motivating and effective teaching method. <br />
<br />
iGEM began in January of 2003 with a month-long course during MIT's Independent Activities Period (IAP). The students designed biological systems to make cells blink. This design course grew to a summer competition with 5 teams in 2004, 13 teams in 2005 - the first year that the competition grew internationally, 32 teams in 2006, 54 teams in 2007, 84 teams in 2008, and 112 teams in 2009. Projects ranged from a rainbow of pigmented bacteria, to banana and wintergreen smelling bacteria, an arsenic biosensor, Bactoblood, and buoyant bacteria. <br />
<br />
For iGEM 2010 we are expecting about 180 teams participating and over 1800 participants taking part in the competition. They will specify, design, build, and test simple biological systems made from standard, interchangeable biological parts. The accomplishments of these student teams during one summer are often impressive and will lead to important advances in medicine, energy, and the environment. Teams will present their projects at the iGEM Championship Jamboree in November 2010.<br />
<br />
For more information please visit [http://www.igem.org www.igem.org].<br />
<br />
===iGEM Logo===<br />
Download the iGEM logo '''[https://static.igem.org/mediawiki/2008/4/44/Igem_logos.zip here]'''.<br />
Note that the iGEM logo is copyrighted by iGEM and provided here for iGEM participants to help them promote iGEM and their iGEM team.<br />
<br />
<br />
<br />
===iGEM Contact Information===<br />
Please direct all press questions to:<br />
<html><br />
<div style="float:left; padding:20px;"><br />
<a href="https://2008.igem.org/Contact"><br />
<img src="https://static.igem.org/mediawiki/2008/2/21/Randy_contact_small.png"<br />
alt="Randy AT igem DOT org"<br />
title="Randy AT igem DOT org"><br />
</a><br><br />
<strong>Randy Rettberg</strong>, <em>Director</em><br><br />
+1.617.258.5244<br />
</div><br />
<br />
<div style="float:left; padding:20px;"><br />
<a href="https://2008.igem.org/User:Meagan"><br />
<img src="https://static.igem.org/mediawiki/2008/5/5e/Meagan_contact_small.png"<br />
alt="Meagan AT igem DOT org"<br />
title="Meagan AT igem DOT org"><br />
</a><br><br />
<strong>Meagan Lizarazo</strong>, <em>Assistant Director</em><br><br />
+1.617.258.5244<br />
</div><br />
</html></div>Meaganhttp://2009.igem.org/File:Kyotoexperiment.jpgFile:Kyotoexperiment.jpg2009-12-16T16:42:04Z<p>Meagan: uploaded a new version of "Image:Kyotoexperiment.jpg": Reverted to version as of 05:27, 13 December 2009</p>
<hr />
<div></div>Meaganhttp://2009.igem.org/File:Kyotoexperiment.jpgFile:Kyotoexperiment.jpg2009-12-16T16:41:54Z<p>Meagan: uploaded a new version of "Image:Kyotoexperiment.jpg"</p>
<hr />
<div></div>Meaganhttp://2009.igem.org/User:Meagan/Visa_htmlUser:Meagan/Visa html2009-12-11T19:40:54Z<p>Meagan: </p>
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<li id="igem_image"><img src="https://static.igem.org/mediawiki/2009/d/d1/IGEM_basic_Logo_150px.png"></li><br />
<li id="mit_image"><img src="https://static.igem.org/mediawiki/2009/1/10/Mit_logo_mini.jpg"></li><br />
</ul><br />
</div> <br />
<br />
<div id="date"><br />
<span class="date">May 19, 2009</span><br />
</div><br />
<br />
<div id="recipient_information"><br />
<ul><br />
<li class="no_bullet"> <span class="name">Daniel Solís Escalante</span></li><br />
<li class="no_bullet"> <span class="address">Roland Holstlaan</span></li><br />
<li class="no_bullet"> <span class="city">435 2624 HL, Delft</span></li><br />
<li class="no_bullet"> <span class="country">The Netherlands</span></li><br />
<li class="no_bullet">&nbsp;</li><br />
<li class="no_bullet"> Passport number: <span class="passport">07400039122</span></li><br />
<li class="no_bullet"> Citizenship: <span class="citizenship">Mexico</span></li><br />
<li class="no_bullet"> Date of birth: <span class="birth">01/09/1985</span></li><br />
</ul><br />
</div> <br />
<br />
<div id="salutation"><br />
Dear <span class="name">Daniel Solís Escalante</span>,<br />
</div><br />
<br />
<div id="letter_body"><br />
<p><br />
We are pleased to invite you to participate in the 2009 International Genetically Engineered Machine (iGEM) competition this summer and to attend the iGEM 2009 Championship Jamboree October 30 to November 2, 2009 at the Massachusetts Institute of Technology in Cambridge, Massachusetts, USA.<br />
</p><br />
<p><br />
Last summer, eighty-four student teams from schools in Asia, Europe, Latin America, and the US specified, designed, built, and tested simple biological systems made from standard, interchangeable biological parts. They presented their designs and systems at the 2008 Jamboree at MIT. This project design and competition format is an exceptionally motivating and effective teaching method. The accomplishments of a small number of undergraduates during a summer can be quite impressive. <br />
</p><br />
<p><br />
This year iGEM is even bigger with over one hundred teams and we’re sure the Jamboree and the projects presented there will be even more innovative and exciting.<br />
</p><br />
<table id="events"><br />
<th>Schedule of Events</th><br />
<tr><br />
<td><br />
Spring Workshops -<br />
</td><br />
<td><br />
MIT, May 16/17, 2009 – to be held at MIT, Cambridge, MA, USA;<br />
</td><br />
</tr><br />
<tr><br />
<td><br />
&nbsp;<br />
</td><br />
<td><br />
Europe, June 20/21, 2009 – to be held at Imperial College, London, UK;<br />
</td><br />
</tr><br />
<tr><br />
<td><br />
&nbsp;<br />
</td><br />
<td><br />
Asia, June 27/28, 2009 – to be held at University of Tokyo, Tokyo, Japan;<br />
</td><br />
<tr><br />
<td><br />
iGEM Jamboree - <br />
</td><br />
<td><br />
October 30 to November 2, 2009 – to be held at MIT, Cambridge, MA, USA;<br />
</td><br />
</tr><br />
</table><br />
<p><br />
Remember, teams may be any size, but must have at least two instructors and must be composed primarily of undergraduate students. The at least one instructor must attend one of the spring workshops (students are highly encouraged to attend) and all students are expected to attend the Jamboree in November. You can view the complete list of requirements for participation in iGEM 2009 on our web site.<br />
</p><br />
<p><br />
We look forward to seeing you at the spring workshops and at the Jamboree.<br />
</p><br />
</div> <!-- CLOSE letter_body --><br />
<br />
<div id="signature"><br />
<table><br />
<tr><br />
<td><br />
<ul><br />
<li class="no_bullet"></li><br />
<li class="no_bullet">Randy Rettberg</li><br />
<li class="no_bullet">Director,</li><br />
<li class="no_bullet">iGEM / Registry of Standard Biological Parts</li><br />
</ul><br />
</td><br />
<td><br />
<ul><br />
<li class="no_bullet"></li><br />
<li class="no_bullet">Meagan Lizarazo</li><br />
<li class="no_bullet">Assistant Director,</li><br />
<li class="no_bullet">iGEM / Registry of Standard Biological Parts</li><br />
</ul><br />
</td><br />
</tr><br />
</table><br />
<ul id="headquarters"><br />
<li class="no_bullet">iGEM Headquarters</li><br />
<li class="no_bullet">Massachusetts Institute of Technology (MIT)</li><br />
<li class="no_bullet">Cambridge, MA, USA</li><br />
<li class="no_bullet">HQ [AT] igem.org</li><br />
<ul><br />
</div> <!-- CLOSE signature --><br />
<br />
</div> <!-- ###CLOSE LETTER### --><br />
</body><br />
</html></div>Meaganhttp://2009.igem.org/Main_PageMain Page2009-12-10T16:21:49Z<p>Meagan: </p>
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<img src="https://static.igem.org/mediawiki/2009/0/00/Freezer_plates_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 15px;"<br />
alt="Main page image: freezer plates"<br />
title="Repository freezer plates."><br />
<img src="https://static.igem.org/mediawiki/2009/3/3d/Slovenia_mascot_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: Slovenia mascot."<br />
title="iGEM 2008 Slovenia mascot."><br />
<img src="https://static.igem.org/mediawiki/2009/5/57/Igem_banner_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: iGEM banner." <br />
title="iGEM banner."> <br />
<!-- <img src="https://static.igem.org/mediawiki/2009/3/37/Finalist_announce_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: 2008 finalist announcement"<br />
title="Announcing the finalists at the 2008 Jamboree."> --><br />
<!-- <img src="https://static.igem.org/mediawiki/2009/2/23/Dhl_boxes_2_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: DHL boxes"<br />
title="DHL boxes for the DNA distribution."> --><br />
<img src="https://static.igem.org/mediawiki/2009/3/38/Blackboard_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: Tagging blackboard!"<br />
title="Tagging the iGEM blackboard!"><br />
<img src="https://static.igem.org/mediawiki/2009/3/3a/Phage_mascot_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: Heidelberg phage mascot"<br />
title="iGEM 2008 Heidelberg phage mascot."><br />
<!-- <img src="https://static.igem.org/mediawiki/2009/9/9e/Presentation_room_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: 2008 Jamboree presentation room"<br />
title="2008 Jamboree presentation room."> --><br />
<img src="https://static.igem.org/mediawiki/2009/a/ab/Kit_plates_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: DNA distribution kit plates"<br />
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<br />
<div id="news_items" style="width: 700px; margin-left: 20px; float:left;"> <br />
<br />
<!-- *** QUICK LINKS BOX *** --><br />
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<table style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; background-color:#fbefb8;"><br />
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<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> <a class="quick_link" href="https://2009.igem.org/Jamboree">iGEM 2009 Jamboree</a></h3><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center; font-weight:bold;">October 31 to November 2, 2009</p><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;">Massachusetts Institute of Technology</p><br />
<ul style="list-style:none;" ><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Quick links:</lh><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Project_Abstract/Team_Abstracts">Team abstracts</a></li><br />
<li><a class="quick_link" href="https://igem.org/Team_Wikis?year=2009">Team websites</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Schedule">Schedule</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Campus_map">Campus Map</a></li><br />
<li><a class="quick_link" href="https://igem.org/Results?year=2009">iGEM 2009 Jamboree results</li><br />
</ul><br />
</td><br />
</td><br />
<td style="width:325px;"><br />
<img src="https://static.igem.org/mediawiki/2009/9/95/2009_igem_from_above_300.jpg"<br />
style="margin-right:-110px;"><br />
</td><br />
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</table><br />
</div><br />
<br />
<!-- NEWS --><br />
<p style="padding: 10px; margin: 10px; color: #666; font-family: Lucida Grande, Verdana, Arial, sans-serif;"><span style="font-size:115%; font-weight:bold; color:#576f91;">News:</span> Check out the <a href="https://2009.igem.org/Job_Opportunities">new job opportunities</a> at iGEM Headquarters.</p><br />
<br />
<br />
<br />
<!-- ADD YOUR PUBLICITY --><br />
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<td><br />
<p style="font-size:130%; /*font-weight:bold;*/ color:#576f91;">Add your iGEM 2009 <a class="green_link" href="https://2009.igem.org/IGEM_Publicity">publicity</a>, <a class="green_link" href="https://2009.igem.org/Photo_Gallery">photos</a>, & <a class="green_link" href="https://2009.igem.org/IGEM_Publications">publications</a></p><br />
</td><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/1/1a/Star_25.jpg"><br />
</td><br />
</tr><br />
</table><br />
<br />
<!-- *** JAMBOREE RESULTS *** --><br />
<div id="jamboree_results_box" style="width:700px; margin:0px 0px 30px 0px;"><br />
<p style="font-size: 200%; font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#ed0100; text-align:center; margin-left:20px;">iGEM 2009 is officially over!</p><br />
<img src="https://static.igem.org/mediawiki/2009/2/2a/Jamboree2009_cambridge_award.jpg"<br />
style="margin-left:125px; border:2px solid #aaa"><br />
<br />
<!-- GRAND PRIZE, 1ST, 2ND --><br />
<ul style="list-style:none; font-size:110%; color:#666; font-family: Lucida Grande, Verdana, Arial, sans-serif; text-align:center;"><br />
<li><strong>Grand Prize, Winner of the BioBrick Trophy:</strong> Cambridge</li><br />
<li><strong>1st Runner Up:</strong> Heidelberg</li><br />
<li><strong>2nd Runner Up:</strong> Valencia</li><br />
</ul><br />
<br />
<!-- FINALISTS --><br />
<div id="finalist_list"><br />
<ul style="list-style: none;"><br />
<lh>Finalists:</lh><br />
<li>Cambridge</li><br />
<li>Freiburg bioware</li><br />
<li>Groningen</li><br />
<li>Heidelberg</li><br />
<li>Imperial College London</li><br />
<li>Valencia</li><br />
</ul><br />
</div> <!-- close finalists --><br />
<br />
<!-- TRACK AND SPECIAL AWARDS --><br />
<table style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666;"><br />
<tr><br />
<td style="width:350px;"><br />
<ul style="list-style:none;"><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Track Award Winners:</lh><br />
<li><strong>Best Food or Energy Project:</strong> UNIPV-Pavia</li><br />
<li><strong>Best Environment Project:</strong> Cambridge</li><br />
<li><strong>Best Health or Medicine Project:</strong> Stanford</li><br />
<li><strong>Best Manufacturing Project:</strong> Imperial College London</li><br />
<li><strong>Best New Application Area:</strong> Valencia</li><br />
<li><strong>Best Foundation Advance:</strong> Alberta</li><br />
<li><strong>Best Information Processing Project:</strong> TUDelft</li><br />
<li><strong>Best Software Tool:</strong> Berkeley Software & Illinois-Tools <small>(Tie)</small></li><br />
</ul><br />
</td><br />
<td style="width:350px;"><br />
<ul style="list-style:none;"><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Special Prizes Winners:</lh><br />
<li><strong>Best BioBrick Part, Natural:</strong> ULB-Brussels</li><br />
<li><strong>Best New BioBrick Part or Device, Engineered:</strong> EPF-Lausanne & Freiburg Bioware <small>(Tie)</small></li><br />
<li><strong>Best Human Practices Advance:</strong> Imperial College London & Paris <small>(Tie)</small></li><br />
<li><strong>Best Experimental Measurement:</strong> Valencia</li><br />
<li><strong>Best Model:</strong> BCCS-Bristol</li><br />
<li><strong>Best Wiki:</strong> Heidelberg</li><br />
<li><strong>Best Poster:</strong> Freiburg bioware</li><br />
<li><strong>Best Presentation:</strong> ArtScienceBangalore</li><br />
<li><strong>Best New Standard:</strong> Heidelberg</li><br />
</td><br />
</tr><br />
</table><br />
<p style="font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> See the <a href="https://igem.org/Results?year=2009">Results page</a> for details</p><br />
</div> <!-- close Jamboree results box --><br />
<br />
<br />
<br />
<!-- ***** Begin entry style portion of page ***** --><br />
<br />
<br />
<br />
<br />
<!-- *** ARCHIVE *** --><br />
<a href="https://2009.igem.org/Main_page_archive"><small>(News post archives)</small></a><br />
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<div class='right-heading'>About iGEM</div><br />
* [[About | What is iGEM?]]<br />
<!--* [[iGEM_Publicity | News about iGEM]]--><br />
* [[Previous iGEM Competitions | Previous iGEM competitions]]<br />
* [[Contact | iGEM Headquarters]]<br />
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<div class='right-heading'>iGEM Start to Finish</div><br />
* [[Calendar of Events | Calendar of events]]<br />
* [[Start_A_Team | Start a team]]<br />
* [[Requirements]]<br />
* [https://igem.org/IGEM_2009_Registration iGEM 2009 Registration]<br />
* [[Workshops| Spring workshops]]<br />
* [[Summer_events | Summer News & Events]]<br />
* [[Jamboree | The Jamboree]]<br />
** [[Jamboree/Attendance fee | Registration]]<br />
** [[Jamboree/Schedule | Schedule]]<br />
** [[Judging | Judging]]<br />
* <span style="font-weight:bold; font-size:115%;">[[Photo_Gallery | iGEM Photo Gallery]]</span><br />
* <span style="font-weight:bold; font-size:115%;">[[iGEM_Publicity | iGEM Publicity]]</span><br />
* [[iGEM_Publications | iGEM Publications]]<br />
* [[Safety | Safety]]<br />
* [[Security]]<br />
</div> <!-- close Start-to-finish --><br />
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<div class='right-heading'>Resources</div><br />
<!--* [[Mailing_Lists | Mailing lists]]--><br />
* [https://igem.org/Team_List?year=2009 2009 teams]<br />
* [https://2009.igem.org/Team_Wikis?year=2009 2009 team wikis]<br />
* [https://igem.org/Team_Parts?year=2009 2009 team parts]<br />
* [https://igem.org/Team_Judging?year=2009 2009 judging forms]<br />
* [[Partner_Offers | iGEM Partner Offers]]<br />
* [[Instructional Videos]]<br />
* [[Using the wiki]]<br />
* [http://partsregistry.org Registry of Standard Parts]<br />
* [[Main page archive | Main page archive]]<br />
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alt="iGEM forum button."<br />
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<img src="https://static.igem.org/mediawiki/2009/e/ec/Mathworks_Logo_R1_RGB_10per.jpg"<br />
style="padding: 0px 10px 0px 0px;"><br />
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<img src="https://static.igem.org/mediawiki/2009/9/93/Mit_logo_h26px.jpg"<br />
style="padding: 0px 10px 0px 0px;"><br />
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<img src="https://static.igem.org/mediawiki/2009/e/e9/SynBERC_logo_h26px.png"<br />
style="padding: 0px 10px 0px 0px;"><br />
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style="padding: 0px 10px 0px 0px;"><br />
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<a href="https://2009.igem.org/Sponsors"><br />
<img src="https://static.igem.org/mediawiki/2009/6/6f/BBF_h30px.png"<br />
style="padding: 0px 10px 0px 0px;"><br />
</td><br />
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</table><br />
--><br />
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<img src="https://static.igem.org/mediawiki/2009/d/df/Sponsor_footer_trim.png"<br />
style="padding-left:10px;"<br />
alt="iGEM 2009 Sponsors"<br />
title="iGEM 2009 Sponsors"><br />
</a><br />
<a href="https://2009.igem.org/Sponsorship"><br />
<img src="https://static.igem.org/mediawiki/2009/8/8d/Sponsor_now_h35px.png"<br />
style="padding-left:10px;"<br />
alt="Become an iGEM sponsor"<br />
title="Become an iGEM sponsor"><br />
</a><br />
</div><br />
</html></div>Meaganhttp://2009.igem.org/Main_PageMain Page2009-12-10T16:21:09Z<p>Meagan: </p>
<hr />
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<br />
<img src="https://static.igem.org/mediawiki/2009/0/00/Freezer_plates_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 15px;"<br />
alt="Main page image: freezer plates"<br />
title="Repository freezer plates."><br />
<img src="https://static.igem.org/mediawiki/2009/3/3d/Slovenia_mascot_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: Slovenia mascot."<br />
title="iGEM 2008 Slovenia mascot."><br />
<img src="https://static.igem.org/mediawiki/2009/5/57/Igem_banner_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: iGEM banner." <br />
title="iGEM banner."> <br />
<!-- <img src="https://static.igem.org/mediawiki/2009/3/37/Finalist_announce_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: 2008 finalist announcement"<br />
title="Announcing the finalists at the 2008 Jamboree."> --><br />
<!-- <img src="https://static.igem.org/mediawiki/2009/2/23/Dhl_boxes_2_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: DHL boxes"<br />
title="DHL boxes for the DNA distribution."> --><br />
<img src="https://static.igem.org/mediawiki/2009/3/38/Blackboard_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: Tagging blackboard!"<br />
title="Tagging the iGEM blackboard!"><br />
<img src="https://static.igem.org/mediawiki/2009/3/3a/Phage_mascot_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: Heidelberg phage mascot"<br />
title="iGEM 2008 Heidelberg phage mascot."><br />
<!-- <img src="https://static.igem.org/mediawiki/2009/9/9e/Presentation_room_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: 2008 Jamboree presentation room"<br />
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<img src="https://static.igem.org/mediawiki/2009/a/ab/Kit_plates_xsmall.jpg"<br />
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<br />
<div id="news_items" style="width: 700px; margin-left: 20px; float:left;"> <br />
<br />
<!-- *** QUICK LINKS BOX *** --><br />
<div id="jamboree_highlight_box" style="border:3px solid #fed801; width:700px; margin:0px 0px 30px 0px; padding:5px; background-color:#fbefb8;"><br />
<table style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; background-color:#fbefb8;"><br />
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<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> <a class="quick_link" href="https://2009.igem.org/Jamboree">iGEM 2009 Jamboree</a></h3><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center; font-weight:bold;">October 31 to November 2, 2009</p><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;">Massachusetts Institute of Technology</p><br />
<ul style="list-style:none;" ><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Quick links:</lh><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Project_Abstract/Team_Abstracts">Team abstracts</a></li><br />
<li><a class="quick_link" href="https://igem.org/Team_Wikis?year=2009">Team websites</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Schedule">Schedule</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Campus_map">Campus Map</a></li><br />
<li><a class="quick_link" href="https://igem.org/Results?year=2009">iGEM 2009 Jamboree results</li><br />
</ul><br />
</td><br />
</td><br />
<td style="width:325px;"><br />
<img src="https://static.igem.org/mediawiki/2009/9/95/2009_igem_from_above_300.jpg"<br />
style="margin-right:-110px;"><br />
</td><br />
</tr><br />
</table><br />
</div><br />
<br />
<!-- NEWS --><br />
<p style="padding: 10px; margin: 10px; color: #666; font-family: Lucida Grande, Verdana, Arial, sans-serif;"><span style="font-size:115%; font-weight:bold; color:#576f91;">News:</span> New job opportunities at iGEM Headquarters. More details <a href="https://2009.igem.org/Job_Opportunities">here</a>.</p><br />
<br />
<br />
<br />
<!-- ADD YOUR PUBLICITY --><br />
<table id="publicity_table"><br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/1/1a/Star_25.jpg"><br />
</td><br />
<td><br />
<p style="font-size:130%; /*font-weight:bold;*/ color:#576f91;">Add your iGEM 2009 <a class="green_link" href="https://2009.igem.org/IGEM_Publicity">publicity</a>, <a class="green_link" href="https://2009.igem.org/Photo_Gallery">photos</a>, & <a class="green_link" href="https://2009.igem.org/IGEM_Publications">publications</a></p><br />
</td><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/1/1a/Star_25.jpg"><br />
</td><br />
</tr><br />
</table><br />
<br />
<!-- *** JAMBOREE RESULTS *** --><br />
<div id="jamboree_results_box" style="width:700px; margin:0px 0px 30px 0px;"><br />
<p style="font-size: 200%; font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#ed0100; text-align:center; margin-left:20px;">iGEM 2009 is officially over!</p><br />
<img src="https://static.igem.org/mediawiki/2009/2/2a/Jamboree2009_cambridge_award.jpg"<br />
style="margin-left:125px; border:2px solid #aaa"><br />
<br />
<!-- GRAND PRIZE, 1ST, 2ND --><br />
<ul style="list-style:none; font-size:110%; color:#666; font-family: Lucida Grande, Verdana, Arial, sans-serif; text-align:center;"><br />
<li><strong>Grand Prize, Winner of the BioBrick Trophy:</strong> Cambridge</li><br />
<li><strong>1st Runner Up:</strong> Heidelberg</li><br />
<li><strong>2nd Runner Up:</strong> Valencia</li><br />
</ul><br />
<br />
<!-- FINALISTS --><br />
<div id="finalist_list"><br />
<ul style="list-style: none;"><br />
<lh>Finalists:</lh><br />
<li>Cambridge</li><br />
<li>Freiburg bioware</li><br />
<li>Groningen</li><br />
<li>Heidelberg</li><br />
<li>Imperial College London</li><br />
<li>Valencia</li><br />
</ul><br />
</div> <!-- close finalists --><br />
<br />
<!-- TRACK AND SPECIAL AWARDS --><br />
<table style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666;"><br />
<tr><br />
<td style="width:350px;"><br />
<ul style="list-style:none;"><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Track Award Winners:</lh><br />
<li><strong>Best Food or Energy Project:</strong> UNIPV-Pavia</li><br />
<li><strong>Best Environment Project:</strong> Cambridge</li><br />
<li><strong>Best Health or Medicine Project:</strong> Stanford</li><br />
<li><strong>Best Manufacturing Project:</strong> Imperial College London</li><br />
<li><strong>Best New Application Area:</strong> Valencia</li><br />
<li><strong>Best Foundation Advance:</strong> Alberta</li><br />
<li><strong>Best Information Processing Project:</strong> TUDelft</li><br />
<li><strong>Best Software Tool:</strong> Berkeley Software & Illinois-Tools <small>(Tie)</small></li><br />
</ul><br />
</td><br />
<td style="width:350px;"><br />
<ul style="list-style:none;"><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Special Prizes Winners:</lh><br />
<li><strong>Best BioBrick Part, Natural:</strong> ULB-Brussels</li><br />
<li><strong>Best New BioBrick Part or Device, Engineered:</strong> EPF-Lausanne & Freiburg Bioware <small>(Tie)</small></li><br />
<li><strong>Best Human Practices Advance:</strong> Imperial College London & Paris <small>(Tie)</small></li><br />
<li><strong>Best Experimental Measurement:</strong> Valencia</li><br />
<li><strong>Best Model:</strong> BCCS-Bristol</li><br />
<li><strong>Best Wiki:</strong> Heidelberg</li><br />
<li><strong>Best Poster:</strong> Freiburg bioware</li><br />
<li><strong>Best Presentation:</strong> ArtScienceBangalore</li><br />
<li><strong>Best New Standard:</strong> Heidelberg</li><br />
</td><br />
</tr><br />
</table><br />
<p style="font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> See the <a href="https://igem.org/Results?year=2009">Results page</a> for details</p><br />
</div> <!-- close Jamboree results box --><br />
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* [[About | What is iGEM?]]<br />
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<div class='right-heading'>iGEM Start to Finish</div><br />
* [[Calendar of Events | Calendar of events]]<br />
* [[Start_A_Team | Start a team]]<br />
* [[Requirements]]<br />
* [https://igem.org/IGEM_2009_Registration iGEM 2009 Registration]<br />
* [[Workshops| Spring workshops]]<br />
* [[Summer_events | Summer News & Events]]<br />
* [[Jamboree | The Jamboree]]<br />
** [[Jamboree/Attendance fee | Registration]]<br />
** [[Jamboree/Schedule | Schedule]]<br />
** [[Judging | Judging]]<br />
* <span style="font-weight:bold; font-size:115%;">[[Photo_Gallery | iGEM Photo Gallery]]</span><br />
* <span style="font-weight:bold; font-size:115%;">[[iGEM_Publicity | iGEM Publicity]]</span><br />
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</div> <!-- close Start-to-finish --><br />
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<!--* [[Mailing_Lists | Mailing lists]]--><br />
* [https://igem.org/Team_List?year=2009 2009 teams]<br />
* [https://2009.igem.org/Team_Wikis?year=2009 2009 team wikis]<br />
* [https://igem.org/Team_Parts?year=2009 2009 team parts]<br />
* [https://igem.org/Team_Judging?year=2009 2009 judging forms]<br />
* [[Partner_Offers | iGEM Partner Offers]]<br />
* [[Instructional Videos]]<br />
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* [[Main page archive | Main page archive]]<br />
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style="padding-left:10px;"<br />
alt="Become an iGEM sponsor"<br />
title="Become an iGEM sponsor"><br />
</a><br />
</div><br />
</html></div>Meaganhttp://2009.igem.org/Job_OpportunitiesJob Opportunities2009-12-10T16:20:55Z<p>Meagan: New page: <html> <head> <style> #announcement_box { border: 3px solid #096; width: 800px; margin: 0px 0px 30px 75px; padding: 5px; background-color: #a7d5a2; } #announcement_box p { font-famil...</p>
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<p>Graduating soon? Looking for a job? Check out the new...</p><br />
<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> Job Opportunities at iGEM Headquarters</h3><br />
<p>iGEM Headquarters is looking for someone to help make iGEM great.</p> <br />
<p>Responsibilities include:</p><br />
<ul style="/*list-style:none;*/" ><br />
<li>Improving the quality of parts in the Registry (requires high-level knowledge of standard parts and biology)</li><br />
<li>Developing education materials</li><br />
<li>Developing instructional videos</li><br />
<li>Following the "make iGEM (and the Registry, and synthetic biology...) better" philosophy</li><br />
</ul><br />
<p style="text-align:left; padding-left:20px;">Please send your resume and a letter of intent stating why you would be perfect for the job to <strong>jobs AT igem dot org</strong>.</p><br />
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</html></div>Meaganhttp://2009.igem.org/IGEM_PublicityIGEM Publicity2009-12-09T19:11:05Z<p>Meagan: </p>
<hr />
<div>__NOTOC__<br />
[https://2008.igem.org/IGEM_Publicity iGEM 2008 Publicity] | [http://parts.mit.edu/igem07/index.php/Media iGEM 2007 Publicity] | [http://parts.mit.edu/wiki/index.php/IGEM_News iGEM 2006 Publicity]<br />
<br />
<div style="color:#aaa; padding-bottom:20px;">(Members of the press, please see the [[Press_Kit | iGEM Press Kit]])</div><br />
<br />
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<br />
<br />
<span style="color:#1e90ff; font-size:175%">'''blogs </span><span style="color:#3cb371; font-size:175%">covering iGEM 2009'''</span><br />
* The [http://igem.sdu.dk/ <span style="color:#453221; font-size: 130%">'''SDU-Denmark'''</span>] team blog about their iGEM experience.<br />
* A blog about the iGEM project of the [http://aboutgmos.org/iGEM <span style="color:#453221; font-size: 130%">'''Uppsala-Sweden'''</span>] team.<br />
<br />
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<br />
====<font size=5><font color=dodgerblue>'''video/radio</font><font color=mediumseagreen> about iGEM 2009'''</font></font>====<br />
*'''Slovenia, Heidelberg, SDU-Denmark''': [http://www.dradio.de/dlf/sendungen/forschak/1062608/ Die Biobastler von Boston], Nov 2, 2009, Deutschlandfunk radio (in German).<br />
*'''Cambridge, Imperial''': [http://www.sciencefriday.com/program/archives/200911063 Synthetic Biology Competition], Nov 6, 2009, NPR Science Friday (in English).<br />
<br />
<br />
<br />
<br />
====<font size=5><font color=dodgerblue>'''news articles</font><font color=mediumseagreen> about iGEM 2009'''</font></font>====<br />
<br />
<br />
If you would like to share an article that was written about iGEM or your iGEM team, please link to it on this page. If you have multiple articles featuring your team, link to them all individually!<br />
<br />
Post the name of your team, the title of your article, where it was featured, and provide a link to it. <br />
<br />
''Example'':<br> <br />
'''Team Example''': ''Title of article'', Nature, [link]<br />
<br />
====<font size=4><font color=dodgerblue>'''general'''</font></font>====<br />
*'''The Economist''': ''Biohacking: Hacking goes squishy'' Sep 3, 2009 [http://www.economist.com/search/displaystory.cfm?story_id=14299634 The Economist]<br />
*'''The Scientist''': ''Brick by Brick'' Feb 1, 2009. [http://www.the-scientist.com/article/display/55378/ The Scientist]<br />
*'''Technology Review''': ''A Genetically Engineered Rainbow of Bacteria'' Nov 03, 2009. [http://www.technologyreview.com/blog/editors/24351/ Technology Review]<br />
*'''Discovery News''': '' Bright Bacteria Wins Synthetic Biology Competition'' Nov 6, 2009. [http://blogs.discoverychannel.co.uk/discovery-news/2009/11/bright-bacteria-wins-synthetic-biology-competition.html Discovery News]<br />
*'''Wired''': '' Building new life forms at the iGEM Jamboree'' Nov 9, 2009. [http://www.wired.co.uk/news/archive/2009-11/09/building-new-life-forms-at-the-igem-jamboree.aspx Wired]<br />
*'''Nature Biotechnology''': ''Building outside of the box: iGEM and the BioBricks Foundation'' [http://www.nature.com/nbt/journal/v27/n12/full/nbt1209-1099.html Nature Biotechnology 27, 1099 - 1102 (2009)]<br />
<br />
<br />
====<font size=4><font color=dodgerblue>'''team specific'''</font></font>====<br />
*'''[[Team:IBB_Pune]]''': "<i>Bio-champs in the making</i>" (English), [http://www.punemirror.in/index.aspx?Page=article&sectname=News%20-%20City&sectid=2&contentid=200909142009091423331646a131eacc#ftr2 (Monday, September 14, 2009 at 11:33:25 PM)]<br />
*'''[[Team:IBB_Pune]]''': "<i>Ganiti Prakriyanmadhe Jeevanuncha Vaapar! (Use of Bacteria in mathematical devices)</i>" (Marathi), [http://beta.esakal.com/2009/09/09220618/pune-use-of-bacterias-in-maths.html(Wednesday, September 09th, 2009 AT 10:09 PM)]<br />
*'''[[Team:IBB_Pune]]''': "<i>Synthetic Biologiteel Bharari ("Advances in Synthetic Biology")</i>" (Marathi), [https://2009.igem.org/Team:IBB_Pune/press (Wednesday 7 October 2009)]<br />
<br />
*'''[[Team:Groningen]]''': "<i>Students from Groningen in competition for best bacteria</i>" ([http://www.cityoftalent.nl/en/content/nieuws/nieuws/groningse-studenten-synthetische-biologie-maken-bacterie English]/[http://www.cityoftalent.nl/nl/content/nieuws/nieuws/groningse-studenten-synthetische-biologie-maken-bacterie Dutch]), [http://www.cityoftalent.nl/en/content/nieuws/nieuws/groningse-studenten-synthetische-biologie-maken-bacterie City of Talent (August 20, 2009)]<br />
*'''[[Team:Groningen]]''': "<i>Talent of the Purest Water</i>" (Dutch, advertisement) [http://www.volkskrant.nl/vk-online/VK/20091024___/1_003/ad6.html Volkskrant, (October 24 2009)]<br />
*'''[[Team:Groningen]]''': "<i>RUG-team in finals synthetic biology</i>" (Dutch), [http://www.uk.rug.nl/cms-basis/nieuws.php?subaction=showfull&id=1257343172&archive=&start_from=&ucat=1 UK (Paper of the Groningen University) (November 4, 2009)]<br />
*'''[[Team:Groningen]]''': "<i>Team iGEM Groningen has been awarded a Gold Medal</i>" ([http://www.cityoftalent.nl/en/content/nieuws/nieuws/team-igem-groningen-wint-gouden-medaille English]/[http://www.cityoftalent.nl/nl/content/nieuws/nieuws/team-igem-groningen-wint-gouden-medaille Dutch]), [http://www.cityoftalent.nl/en/content/nieuws/nieuws/team-igem-groningen-wint-gouden-medaille City of Talent (November 10, 2009)]<br />
*'''[[Team:Groningen]]''': "<i>Gold and a place in the finals for Groningen iGEM 2009 participants</i>" ([http://www.rug.nl/corporate/nieuws/archief/archief2009/persberichten/174_09?lang=en English]/[http://www.rug.nl/Corporate/nieuws/archief/archief2009/persberichten/174_09 Dutch]), [http://www.rug.nl/Corporate/nieuws/archief/archief2009/persberichten/174_09?lang=en Press release University Groningen (November 11, 2009)]<br />
*'''[[Team:Groningen]]''': "<i>Performance of world class RUG in Boston</i>" (Dutch), [http://www.groningergezinsbode.nl/profile/redactiegroningergezinsbode/article73205.ece/wereldprestatie_rug_in_boston Groninger Gezinsbode (November 11, 2009)]<br />
*'''[[Team:Groningen]]''': "<i>Groningen students finalists gentech-competition</i>" (Dutch), [http://www.studned.nl/1084/wetenschap/groningse-studenten-finalisten-gentech-wedstrijd StudNed.nl (November 11, 2009)]<br />
*'''[[Team:Groningen]]''': "<i>International price for Groningen students</i>" (Dutch), [http://www.rtvnoord.nl/nieuws/indexwm.asp?actie=totaalbericht&pid=86497 RTV-Noord (November 11, 2009)]<br />
*'''[[Team:Groningen]]''': "<i>Gold and a place in the finals for Groningen contestants iGEM 2009</i>" (Dutch), [http://www.noorderlink.nl/nieuws/goud-en-een-finale-plaats-voor-groningse-deelnemers-igem-2009 NoorderLink (November 11, 2009)]<br />
*'''[[Team:Groningen]]''': "<i>Gold and a place in the finals for Groningen contestants iGEM 2009</i>" (Dutch), [http://www.headlinez.nl/?nr=2295334 HeadLinez.nl (November 11, 2009)]<br />
*'''[[Team:Groningen]]''': "<i>With a pacmanbacterium in de finals</i>" (Dutch), [http://www.uk.rug.nl/archief/jaargang39/11/12e.php UK (Paper of the Groningen University) (November 12, 2009)]<br />
*'''[[Team:Groningen]]''' & '''[[Team:TUDelft]]''': "<i>iGEM Gold for Dutch</i>" (Dutch), [http://www.bionieuws.nl/artikel.php?id=4918&zoek=iGEM Bionieuws (November 14, 2009)]<br />
*'''[[Team:Paris]]''' : "<i>Un gène éthique qui vaut de l'or</i>" Le Monde, (French), [http://www.lemonde.fr/planete/article/2009/11/27/un-gene-ethique-qui-vaut-de-l-or_1272940_3244.html (November 27, 2009)]<br />
*'''[[Team:SupBiotech-Paris]]''': "<i>Un concours organisé par le M.I.T : en route pour Boston ! </i>" (French), [http://www.supbiotech.fr/2009/09/boston-supbiotech-igem.html (September 08, 2009)]<br />
<br />
*'''[[Team:SupBiotech-Paris]]''': "<i>Les étudiants de la biotech interpellent la biologie synthétique</i>" (French), [http://www.vivagora.org/spip.php?breve210 (October 09, 2009)]<br />
<br />
*'''[[Team:UAB-Barcelona]]''': "<i>Un equipo de la UAB, en el concurso de biología sintética del MIT</i>" (Spanish), [http://www.uab.es/servlet/Satellite?cid=1096481466568&pagename=UABDivulga%2FPage%2FTemplatePageDetallArticleInvestigar&param1=1253860327385 (September 23, 2009)]<br />
<br />
*'''[[Team:UAB-Barcelona]]''': "<i>UAB to participate in the synthetic biology competition at MIT</i>" (English), [http://www.uab.es/servlet/Satellite/latest-news/news-detail/uab-to-participate-in-the-synthetic-biology-competition-at-mit-1096476786473.html?noticiaid=1253657853358 (September 23, 2009)]<br />
<br />
*'''[[Team:Valencia]]''': "<i>TheValencia team is awarded the Synthetic Standard Prize</i>" (Spanish), [http://www.elpais.com/articulo/sociedad/Levaduras/funcionan/pixeles/elpepuespval/20091103elpepusoc_9/Tes]<br />
<br />
*'''[[Team:Cambridge]]''': "<i>University of Cambridge team wins iGEM synthetic biology competition </i>" (English), [http://www.biotechniques.com/news/University-of-Cambridge-team-wins-iGEM-synthetic-biology-competition/biotechniques-180278.html (November 5 2009)]<br />
<br />
*'''[[Team:Virginia]]''': ''[http://www.washingtonpost.com/wp-dyn/content/article/2009/10/22/AR2009102204628.html New works of science nonfiction]'', The Washington Post (October 23, 2009)<br />
<br />
*'''[[Team:TUDelft]]''': ''Estafette voor bacterien'',(Dutch) Algemeen Dagblad (November 6, 2009)<br />
<br />
*'''[[Team:TUDelft]]''': ''Bacterie-estafette'', (Dutch) [http://noorderlicht.vpro.nl/themasites/mediaplayer/index.jsp?media=42722491&refernr=42560747&portalnr=3626936&hostname=noorderlicht&mediatype=audio&portalid=noorderlicht# Podcast van Radio 1, VPRO Noorderlicht, Annemieke Smit] (November 9, 2009)<br />
<br />
*'''[[Team:TUDelft]]''': ''Bacterie-estafette wint goud en award'', (Dutch) [http://www.tudelft.nl/live/pagina.jsp?id=ee44be90-289e-4efe-8a12-4636f1c86b28&lang=nl TNW Today Editie 2] (November 5, 2009)<br />
<br />
*'''[[Team:TUDelft]]''': ''Bacterie-estafette wint goud en award'', (Dutch) [http://www.tudelft.nl/live/pagina.jsp?id=ee44be90-289e-4efe-8a12-4636f1c86b28&lang=nl TUDelft Nieuws] (November 4, 2009)<br />
<br />
*'''[[Team:TUDelft]]''': ''Bacterial relay race wins gold and award'', [http://www.tudelft.nl/live/pagina.jsp?id=26f61b2e-5a3f-40be-b737-a460b08bced7&lang=en Delft University of Technology Press release] (November 4, 2009)<br />
<br />
*'''[[Team:TorontoMaRSDiscovery]]''': ''A gem of a genetics competition'', [http://www.news.utoronto.ca/science-and-technology/a-gem-of-a-genetics-competition.html University of Toronto eBulletin] (November 23, 2009)<br />
<br />
*'''[[Team:Edinburgh]]''':"<i>Synthetic biology against land mines</i>" (Dutch), [http://www.c2w.nl/synthetische-biologie-tegen-landmijnen.72122.lynkx C<sub>2</sub>W Life Sciences (November 17 2009)]</div>Meaganhttp://2009.igem.org/User:Meagan/job_oppsUser:Meagan/job opps2009-12-09T18:27:34Z<p>Meagan: </p>
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<p>Graduating soon? Looking for a job? Check out the new...</p><br />
<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> Job Opportunities at iGEM Headquarters</h3><br />
<p>iGEM Headquarters is looking for someone to help make iGEM great.</p> <br />
<p>Responsibilities include:</p><br />
<ul style="/*list-style:none;*/" ><br />
<li>Improving the quality of parts in the Registry (requires high-level knowledge of standard parts and biology)</li><br />
<li>Developing education materials</li><br />
<li>Developing instructional videos</li><br />
<li>Following the "make iGEM (and the Registry, and synthetic biology...) better" philosophy</li><br />
</ul><br />
<p style="text-align:left; padding-left:20px;">Please send your resume and a letter of intent stating why you would be perfect for the job to <strong>jobs AT igem dot org</strong>.</p><br />
</td><br />
<td style="width:325px;"><br />
<img src="https://static.igem.org/mediawiki/2009/1/1f/Desk_chair_bw_mod_200px.jpg"<br />
style="margin-left:50px;"><br />
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<hr />
<div></div>Meaganhttp://2009.igem.org/User:Meagan/job_oppsUser:Meagan/job opps2009-12-09T18:22:15Z<p>Meagan: </p>
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<p>Graduating soon? Looking for a job? Check out the new...</p><br />
<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> Job Opportunities at iGEM Headquarters</h3><br />
<p>iGEM Headquarters is looking for someone to help make iGEM great.</p> <br />
<p>Responsibilities include:</p><br />
<ul style="/*list-style:none;*/" ><br />
<li>Improving the quality of parts in the Registry (requires high-level knowledge of standard parts and biology)</li><br />
<li>Developing education materials</li><br />
<li>Developing instructional videos</li><br />
<li>Following the "make iGEM (and the Registry, and synthetic biology...) better" philosophy</li><br />
</ul><br />
<p style="text-align:left; padding-left:20px;">Please send your resume and a letter of intent stating why you would be perfect for the job to <strong>jobs AT igem dot org</strong>.</p><br />
</td><br />
<td style="width:325px;"><br />
<img src="https://static.igem.org/mediawiki/2009/b/bc/Desk_chair_bw_200px.jpg"<br />
style="margin-left:50px;"><br />
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<td style="width:475px;"><br />
<p>Graduating soon? Looking for a job? Check out the new...</p><br />
<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> Job Opportunities at iGEM Headquarters</h3><br />
<p>iGEM Headquarters is looking for someone to help make iGEM great.</p> <br />
<p>The responsibilities would include:</p><br />
<ul style="/*list-style:none;*/" ><br />
<li>Curating parts in the Registry (requires high-level knowledge of standard parts and biology)</li><br />
<li>Developing education materials</li><br />
<li>Developing instructional videos</li><br />
<li>Following the "make iGEM (and the Registry, and synthetic biology...) better" philosophy</li><br />
</ul><br />
<p style="text-align:left; padding-left:20px;">Please send your resume and a letter of intent stating why you would be perfect for the job to <strong>jobs AT igem dot org</strong>.</p><br />
</td><br />
<td style="width:325px;"><br />
<img src="https://static.igem.org/mediawiki/2009/b/bc/Desk_chair_bw_200px.jpg"<br />
style="margin-left:50px;"><br />
</td><br />
</tr><br />
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</div><br />
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</html></div>Meaganhttp://2009.igem.org/File:Desk_chair_bw_200px.jpgFile:Desk chair bw 200px.jpg2009-12-09T17:07:27Z<p>Meagan: </p>
<hr />
<div></div>Meaganhttp://2009.igem.org/User:Meagan/job_oppsUser:Meagan/job opps2009-12-08T21:46:07Z<p>Meagan: </p>
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<p>Graduating soon? Looking for a job? Check out the new...</p><br />
<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> Job Opportunities at iGEM Headquarters</h3><br />
<p>iGEM Headquarters is looking for someone to help make iGEM great.</p> <br />
<p>The responsibilities would include:</p><br />
<ul style="/*list-style:none;*/" ><br />
<li>Curating parts in the Registry (requires high-level knowledge of standard parts and biology)</li><br />
<li>Developing education materials</li><br />
<li>Developing instructional videos</li><br />
<li>Following the "make iGEM (and the Registry, and synthetic biology...) better" philosophy</li><br />
</ul><br />
<p style="text-align:left;">Please send your resume and a letter of intent stating why you would be perfect for the job to <strong>jobs AT igem dot org</strong>.</p><br />
</td><br />
<td style="width:325px;"><br />
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<hr />
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font-family: Lucida Grande, Verdana, Arial, sans-serif; <br />
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<td style="width:475px;"><br />
<p>Graduating soon? Looking for a job? Check out the new...</p><br />
<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> Job Opportunities at iGEM Headquarters</h3><br />
<p>iGEM Headquarters is looking for someone to help make iGEM great.</p> <br />
<p>The responsibilities would include:</p><br />
<ul style="/*list-style:none;*/" ><br />
<li>Curating parts in the Registry (requires high-level knowledge of standard parts and biology)</li><br />
<li>Developing education materials</li><br />
<li>Developing instructional videos</li><br />
<li>Following the "make iGEM (and the Registry, and synthetic biology...) better" philosophy</li><br />
</ul><br />
<p style="text-align:left;">Please send your resume and a letter of intent stating why you would be perfect for the job to <strong>jobs AT igem dot org</strong>.</p><br />
</td><br />
<td style="width:325px;"><br />
<!-- ********* NEED TO CHANGE PHOTO!! ********** --><br />
<img src="https://static.igem.org/mediawiki/2009/9/95/2009_igem_from_above_300.jpg"<br />
style="margin-right:-110px;"><br />
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<hr />
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<tr><br />
<td style="width:475px;"><br />
<p>Graduating soon? Looking for a job? Check out the new...</p><br />
<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> Job Opportunities at iGEM Headquarters</h3><br />
<p>iGEM Headquarters is looking for someone to help make iGEM great.</p> <br />
<p>The responsibilities would include:</p><br />
<ul style="/*list-style:none;*/" ><br />
<li>Curating parts in the Registry (requires high-level knowledge of standard parts and biology)</li><br />
<li>Developing education materials</li><br />
<li>Developing instructional videos</li><br />
<li>Following the "make iGEM (and the Registry, and synthetic biology...) better" philosophy</li><br />
</ul><br />
<p>Please send your resume and a letter of intent stating why you would be perfect for the job to <strong>jobs AT igem dot org</strong>.</p><br />
</td><br />
<td style="width:325px;"><br />
<img src="https://static.igem.org/mediawiki/2009/9/95/2009_igem_from_above_300.jpg"<br />
style="margin-right:-110px;"><br />
</td><br />
</tr><br />
</table><br />
</div><br />
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</body><br />
</html></div>Meaganhttp://2009.igem.org/User:Meagan/job_oppsUser:Meagan/job opps2009-12-08T20:57:08Z<p>Meagan: New page: <html> <head> <style> #jamboree_highlight_box ul { margin-left: 150px; } #jamboree_highlight_box ul lh { font-size: 110%; } #jamboree_highlight_box ul li { margin-left: -20px; } </styl...</p>
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<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> <a class="quick_link" href="https://2009.igem.org/Jamboree">iGEM 2009 Jamboree</a></h3><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center; font-weight:bold;">October 31 to November 2, 2009</p><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;">Massachusetts Institute of Technology</p><br />
<ul style="list-style:none;" ><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Quick links:</lh><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Project_Abstract/Team_Abstracts">Team abstracts</a></li><br />
<li><a class="quick_link" href="https://igem.org/Team_Wikis?year=2009">Team websites</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Schedule">Schedule</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Campus_map">Campus Map</a></li><br />
<li><a class="quick_link" href="https://igem.org/Results?year=2009">iGEM 2009 Jamboree results</li><br />
</ul><br />
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<hr />
<div>Testing to see if I can edit --[[User:Meagan|Meagan]] 03:35, 22 October 2009 (UTC)<br />
<br />
[[Image:Meagan biobrick xsmall.jpg|frame|center]]<br />
<br />
Hi everyone. I am Assistant Director of iGEM and the Registry of Standard Biological Parts. <br />
<br><br />
<br><br />
Feel free to contact me at: <br />
<br><br />
<br><br />
meagan {swirly 'at' sign} igem {D-O-T} org<br />
<br />
----<br />
<br />
[[Team:Example | Team 'Example' wiki]]<br />
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[[User:Meagan/Non-traditional_teams | Non-traditional teams]]<br />
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[[Jamboree/Attendance_Fee]]<br />
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[[Safety]]<br />
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[[Judging]]<br />
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[[Registration Handbook]]<br />
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[[User:Meagan/Registration_main_page | Reg page]]<br />
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[[User:Meagan/Workshops | Workshops]]<br />
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[[User:Meagan/Image_scroller | Image scroller]]<br />
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[[User:Meagan/Image_scroller/2 | Image scroller 2]]<br />
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[[User:Meagan/Toggle | Toggle test]]<br />
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[[User:Meagan/Visa_html | Visa letter - html version]]<br />
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[[User:Meagan/Images | Images]]<br />
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[[User:Meagan/Main_page/Summer_events_post | Summer events post]]<br />
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[[User:Meagan/Main_page/Flu | flu]]<br />
<br />
[[User:Meagan/Main_page/judging_form | judging form]]<br />
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[[User:Meagan/Main_page/Testing | main page testing]]<br />
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[[User:Meagan/job_opps | job opps]]<br />
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&action=raw<br />
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====Stylesheets====<br />
[[User:Meagan/Stylesheets/Security.css | Security.css]]<br />
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====Templates====<br />
[[Template:Security_Comments]]<br />
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====Scripts====<br />
[[User:Meagan/Scripts/jquery-1.2.6.pack.js | jquery-1.2.6.pack.js]]<br />
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[[User:Meagan/Scripts/stepcarousel.js | stepcarousel.js]]</div>Meaganhttp://2009.igem.org/IGEM_PublicityIGEM Publicity2009-11-12T19:40:58Z<p>Meagan: </p>
<hr />
<div>__NOTOC__<br />
[https://2008.igem.org/IGEM_Publicity iGEM 2008 Publicity] | [http://parts.mit.edu/igem07/index.php/Media iGEM 2007 Publicity] | [http://parts.mit.edu/wiki/index.php/IGEM_News iGEM 2006 Publicity]<br />
<br />
<div style="color:#aaa; padding-bottom:20px;">(Members of the press, please see the [[Press_Kit | iGEM Press Kit]])</div><br />
<br />
<html><br />
<iframe frameborder="0" width="728" height="90" marginwidth="0" marginheight="0" style="margin:0px 0px 25px 100px;"<br />
src="http://www.google.com/uds/modules/elements/newsshow/iframe.html?q=iGEM&rsz=large&format=728x90"><br />
</iframe><br />
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<br />
<span style="color:#1e90ff; font-size:175%">'''blogs </span><span style="color:#3cb371; font-size:175%">covering iGEM 2009'''</span><br />
* The [http://igem.sdu.dk/ <span style="color:#453221; font-size: 130%">'''SDU-Denmark'''</span>] team blog about their iGEM experience.<br />
* A blog about the iGEM project of the [http://aboutgmos.org/iGEM <span style="color:#453221; font-size: 130%">'''Uppsala-Sweden'''</span>] team.<br />
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====<font size=5><font color=dodgerblue>'''video/radio</font><font color=mediumseagreen> about iGEM 2009'''</font></font>====<br />
*'''Slovenia, Heidelberg, SDU-Denmark''': [http://www.dradio.de/dlf/sendungen/forschak/1062608/ Die Biobastler von Boston], Nov 2, 2009, Deutschlandfunk radio (in German).<br />
*'''Cambridge, Imperial''': [http://www.sciencefriday.com/program/archives/200911063 Synthetic Biology Competition], Nov 6, 2009, NPR Science Friday (in English).<br />
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====<font size=5><font color=dodgerblue>'''news articles</font><font color=mediumseagreen> about iGEM 2009'''</font></font>====<br />
<br />
<br />
If you would like to share an article that was written about iGEM or your iGEM team, please link to it on this page. If you have multiple articles featuring your team, link to them all individually!<br />
<br />
Post the name of your team, the title of your article, where it was featured, and provide a link to it. <br />
<br />
''Example'':<br> <br />
'''Team Example''': ''Title of article'', Nature, [link]<br />
<br />
====<font size=4><font color=dodgerblue>'''general'''</font></font>====<br />
*'''The Economist''': ''Biohacking: Hacking goes squishy'' Sep 3, 2009 [http://www.economist.com/search/displaystory.cfm?story_id=14299634 The Economist]<br />
*'''The Scientist''': ''Brick by Brick'' Feb 1, 2009. [http://www.the-scientist.com/article/display/55378/ The Scientist]<br />
*'''Technology Review''': ''A Genetically Engineered Rainbow of Bacteria'' Nov 03, 2009. [http://www.technologyreview.com/blog/editors/24351/ Technology Review]<br />
*'''Discovery News''': '' Bright Bacteria Wins Synthetic Biology Competition'' Nov 6, 2009. [http://blogs.discoverychannel.co.uk/discovery-news/2009/11/bright-bacteria-wins-synthetic-biology-competition.html Discovery News]<br />
<br />
====<font size=4><font color=dodgerblue>'''team specific'''</font></font>====<br />
<br />
*'''[[Team:IBB_Pune]]''': "<i>Bio-champs in the making</i>" (English), [http://www.punemirror.in/index.aspx?Page=article&sectname=News%20-%20City&sectid=2&contentid=200909142009091423331646a131eacc#ftr2 (Monday, September 14, 2009 at 11:33:25 PM)]<br />
*'''[[Team:IBB_Pune]]''': "<i>Ganiti Prakriyanmadhe Jeevanuncha Vaapar! (Use of Bacteria in mathematical devices)</i>" (Marathi), [http://beta.esakal.com/2009/09/09220618/pune-use-of-bacterias-in-maths.html(Wednesday, September 09th, 2009 AT 10:09 PM)]<br />
*'''[[Team:IBB_Pune]]''': "<i>Synthetic Biologiteel Bharari ("Advances in Synthetic Biology")</i>" (Marathi), [https://2009.igem.org/Team:IBB_Pune/press (Wednesday 7 October 2009)]<br />
<br />
*'''[[Team:Groningen]]''': "<i>Synthetic biology students from Groningen in the race for best bacterium</i>" (Dutch), [http://www.cityoftalent.nl/nl/content/nieuws/nieuws/groningse-studenten-synthetische-biologie-maken-bacterie City of Talent (August 20, 2009)]<br />
*'''[[Team:Groningen]]''': "<i>Talent of the Purest Water</i>" (Dutch, advertisement) [http://www.volkskrant.nl/vk-online/VK/20091024___/1_003/ad6.html Volkskrant, (October 24 2009)]<br />
*'''[[Team:Groningen]]''': "<i>RUG-team in finals synthetic biology</i>" (Dutch), [http://www.uk.rug.nl/cms-basis/nieuws.php?subaction=showfull&id=1257343172&archive=&start_from=&ucat=1 UK (Paper of the Groningen University) (November 4, 2009)]<br />
*'''[[Team:Groningen]]''': "<i>Team iGEM Groningen wins golden medal</i>" (Dutch), [http://www.cityoftalent.nl/nl/content/nieuws/nieuws/team-igem-groningen-wint-gouden-medaille City of Talent (November 10, 2009)]<br />
*'''[[Team:Groningen]]''': "<i>Gold and a place in the finals for Groningen contestants iGEM 2009</i>" (Dutch), [http://www.rug.nl/Corporate/nieuws/archief/archief2009/persberichten/174_09 Press release University Groningen (November 11, 2009)]<br />
*'''[[Team:Groningen]]''': "<i>Performance of world class RUG in Boston</i>" (Dutch), [http://www.groningergezinsbode.nl/profile/redactiegroningergezinsbode/article73205.ece/wereldprestatie_rug_in_boston Groninger Gezinsbode (November 11, 2009)]<br />
*'''[[Team:Groningen]]''': "<i>Groningen students finalists gentech-competition</i>" (Dutch), [http://www.studned.nl/1084/wetenschap/groningse-studenten-finalisten-gentech-wedstrijd StudNed.nl (November 11, 2009)]<br />
*'''[[Team:Groningen]]''': "<i>International price for Groningen students</i>" (Dutch), [http://www.rtvnoord.nl/nieuws/indexwm.asp?actie=totaalbericht&pid=86497 RTV-Noord (November 11, 2009)]<br />
*'''[[Team:Groningen]]''': "<i>Gold and a place in the finals for Groningen contestants iGEM 2009</i>" (Dutch), [http://www.noorderlink.nl/nieuws/goud-en-een-finale-plaats-voor-groningse-deelnemers-igem-2009 NoorderLink (November 11, 2009)]<br />
*'''[[Team:Groningen]]''': "<i>Gold and a place in the finals for Groningen contestants iGEM 2009</i>" (Dutch), [http://www.headlinez.nl/?nr=2295334 HeadLinez.nl (November 11, 2009)]<br />
*'''[[Team:Groningen]]''': "<i>With a pacmanbacterium in de finals</i>" (Dutch), [http://www.uk.rug.nl/archief/jaargang39/11/12e.php UK (Paper of the Groningen University) (November 12, 2009)]<br />
<br />
*'''[[Team:SupBiotech-Paris]]''': "<i>Un concours organisé par le M.I.T : en route pour Boston ! </i>" (French), [http://www.supbiotech.fr/2009/09/boston-supbiotech-igem.html (September 08, 2009)]<br />
<br />
*'''[[Team:SupBiotech-Paris]]''': "<i>Les étudiants de la biotech interpellent la biologie synthétique</i>" (French), [http://www.vivagora.org/spip.php?breve210 (October 09, 2009)]<br />
<br />
*'''[[Team:UAB-Barcelona]]''': "<i>Un equipo de la UAB, en el concurso de biología sintética del MIT</i>" (Spanish), [http://www.uab.es/servlet/Satellite?cid=1096481466568&pagename=UABDivulga%2FPage%2FTemplatePageDetallArticleInvestigar&param1=1253860327385 (September 23, 2009)]<br />
<br />
*'''[[Team:UAB-Barcelona]]''': "<i>UAB to participate in the synthetic biology competition at MIT</i>" (English), [http://www.uab.es/servlet/Satellite/latest-news/news-detail/uab-to-participate-in-the-synthetic-biology-competition-at-mit-1096476786473.html?noticiaid=1253657853358 (September 23, 2009)]<br />
<br />
*'''[[Team:Valencia]]''': "<i>TheValencia team is awarded the Synthetic Standard Prize</i>" (Spanish), [http://www.elpais.com/articulo/sociedad/Levaduras/funcionan/pixeles/elpepuespval/20091103elpepusoc_9/Tes]<br />
<br />
*'''[[Team:Cambridge]]''': "<i>University of Cambridge team wins iGEM synthetic biology competition </i>" (English), [http://www.biotechniques.com/news/University-of-Cambridge-team-wins-iGEM-synthetic-biology-competition/biotechniques-180278.html (November 5 2009)]<br />
<br />
*'''[[Team:Virginia]]''': ''[http://www.washingtonpost.com/wp-dyn/content/article/2009/10/22/AR2009102204628.html New works of science nonfiction]'', The Washington Post (October 23, 2009)</div>Meaganhttp://2009.igem.org/Photo_GalleryPhoto Gallery2009-11-12T15:50:36Z<p>Meagan: </p>
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<div><div style='float:left;width:400px;margin-right:40px;text-align:center;'><br />
[http://www.flickr.com/photos/igemhq/ <font size=4><font color=dodgerblue>'''flick</font><font color=crimson>r&trade;</font> <font color=black>photos'''</font></font>]<br><br />
<small>(Photos are licensed under [http://creativecommons.org/about/licenses Creative Commons - Attribution] license. Please attribute iGEM 2009 Jamboree photos to '''David Appleyard''' and '''iGEM'''.)</small><br />
[http://www.flickr.com/photos/igemhq/ https://static.igem.org/mediawiki/2008/6/6e/Igem_08_blackboard_xsmall.jpg]<br />
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If you upload pictures that you took yourself, use the <font size=3>'''igem'''</font> and <font size=3>'''2009'''</font> tags so that everyone else can find them too.<br />
<div><br />
Post the links to your pictures in the list to the right.<br />
</div><br />
</div><br />
<br />
<br />
<font size=4>'''Pictures taken by the iGEM Community:'''</font><br />
* iGEM Paris [http://picasaweb.google.com/flf.mib/IGEMParisTeam?feat=directlink iGEM Paris]<br />
* iGEM Newcastle [http://www.flickr.com/photos/eridanus/sets/72157622760324890/ iGEM Newcastle]</div>Meaganhttp://2009.igem.org/Jamboree/Project_Abstract/Team_AbstractsJamboree/Project Abstract/Team Abstracts2009-11-05T15:13:14Z<p>Meagan: </p>
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<div>__NOTOC__<br />
<br />
===[[Team:Aberdeen_Scotland | Team Aberdeen_Scotland:]] A Synthetic Biology Approach to Pipe Repair: The Pico-Plumber===<br />
Damage to inaccessible pipe systems, such as computer cooling circuits, is difficult to rectify. An Escherichia coli synthetic biology circuit for pipe repair was designed. Pipe breach detection and the restoration of pipe integrity were implemented through exploitation of chemotaxis, and cell lysis that releases a two-component protein-based glue (lysyl oxidase and tropoelastin). Control was achieved using an AND gate with quorum sensing and the lac inducer IPTG (released from the breach) as inputs. Deterministic and stochastic models of the genetic circuit, integrated with an agent-based model of E.coli cells, were used to define the effective radii of cell migration and timing of lysis. Constructed AND gate, quorum sensing and lysis timing modules were experimentally tested. The two-component glue concept was successfully validated using in vitro alpha-omega complementation of beta-galatosidase activity. Finally, a proposal for an igem.org-based parameter database was developed to aid the rapid identifation of BioBricks parameter values.<br />
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===[[Team:Alberta | Team Alberta:]] A Synthetic Biology Tool Kit for Artificial Genome Design and Construction===<br />
The creation of simplified artificial cells with specialized functions, along design principles that are compatible with the goals of synthetic biology, requires advances in two key areas. In Silico modelling tools are needed to assess the performance of artificial networks prior to assembly. Genome biofabrication must achieve rates well beyond existing methods using a modular design so that the extent to which natural systems can be made artificial can be tested. We have taken our first steps towards these goals by directing our efforts to the rational refactoring of the E. coli genome. Using flux balance analysis we have identified 117 new genes that may be essential for survival. We have developed and validated a rapid, modular biofabrication method (BioBytes) and have produced BioBytes for 150 of our 447 essential gene list. We have also built a Lego Mindstorm-based DIY biofab robot and extended the concept to a BioFab-on-a-chip prototype.<br />
<br />
===[[Team:ArtScienceBangalore | Team ArtScienceBangalore:]]===<br />
We consider ourselves amateurs/novices within the context of the IGEM competition. Our endeavor as “outsiders” is to bring our training in the arts and design to synthetic biology. Over this summer, we learnt the tools and techniques of synthetic biology and developed a piece of life which reflects our concerns, namely, the cultural, ethical and aesthetic implications of Synthetic Biology. Using a DIY approach and getting our hands “wet” was a critical element in the learning process. Our construct synthesizes Geosmin, an enzyme normally produced by cyanobacteria and actinobacteria. The biosynthesis of geosmin from farnesyl diphosphate is catalyzed by a single enzyme germacradienol/germacrene D synthase.E. coli, does not bear a gene that codes for this enzyme. We have expressed this gene in different strains of E. coli. Geosmin is responsible for producing the earthy smell when rain falls after a dry spell of weather.<br />
<br />
===[[Team:Bay_Area_RSI | Team Bay_Area_RSI:]] Breast cancer cell targeting phage===<br />
Breast cancer is the second most common type of cancer diagnosed in women. RNAi has proven to be an effective mechanism in the silencing of oncogenes. Therefore, we have attempted to build a viable system for the delivery of RNAi into breast cancer cells. First, we inserted a shRNA sequence coding for the Raf-1 protein into an AAV cassette containing two ITR's, allowing it to reproduce itself in mammalian cells. This cassette was inserted into our chosen vector, the filamentous bacteriophage FUSE-55. An antibody sequence was then added to the phage plasmid near the coat protein sequence in order to target HER2. As an additional feature, we have fused Silicatein and Silintaphin to mStrepavidin, which will bind to a protein tag in the coat, forming silicate structures on the coat of the phage, thereby reducing the immunotoxicity of the bacteriophage in vivo.<br />
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===[[Team:BCCS-Bristol | Team BCCS-Bristol:]] VESECURE=== <br />
Directed delivery of specific proteins into cells would have dramatic consequences for drug delivery and expand the horizons of synthetic biology into the multicellular domain via discrete, targetted communication. Gram-negative bacteria naturally produce outer member vesicles (OMVs): spherical, bilayered proteolipids from 20-200nm in diameter. OMVs carry outer membrane, periplasmic and cytoplasmic proteins, DNA, RNA and other biological molecules. They protect their cargo from the extracellular environment and deliver it to a multitude of target cells via membrane fusion. We investigate the possibility of allowing the secretion of any protein in OMVs via fusion with novel, non-toxic partners enhanced in OMVs, using a novel Bioscaffold compatible with the current assembly standard. A new version of the award winning BSim software has been developed to study applications at the population level such as communication. The ultimate goal is to create a safe and standardised system for directed delivery of proteins into cells.<br />
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===[[Team:Berkeley_Software | Team Berkeley_Software:]] Eugene, Spectacles, and Kepler: Managing Synthetic Biology Device Development===<br />
Three crucial activities in synthetic biology are the creation of standardized parts, the construction and specification of devices from these parts, and the automatic assembly of these devices. Each of these activities requires software tools. Tools give users access to data as well as provide algorithmic support and abstraction to design large scale systems. We have created three software tools for these tasks. The first is a domain specific language called Eugene for the specification of biological constructs and rules for their creation. The second contribution is a visual design environment for device creation called Spectacles. Finally, we have created workflows for the Kepler design environment. This work is integrated within the Clotho design framework. We show that together they offer a powerful solution to the problems of today while also providing a path to the more exotic design activities of the future.<br />
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===[[Team:Berkeley_Wetlab | Team Berkeley_Wetlab:]] Automated assembly of cell surface display devices===<br />
The University of California Berkeley iGEM team has developed an automated approach to large-scale parts assembly that is accurate, high-throughput, reduces labor, and decreases cost. As a test bed for our system we have chosen to explore novel applications of cell surface display within Escherichia coli, the gold standard organism for bacterial engineering. Displaying peptides and proteins on a cell's surface is difficult, and many attempts may have to be made to generate a given functional protein. By automatically generating and testing a large set of diverse proteins paired with various display methods, we can search a large design space and develop guidelines for rational design of projects involving surface display. <br />
<br />
===[[Team:BIOTEC_Dresden | Team BIOTEC_Dresden:]] Temporal and spatial control of protein synthesis by in vitro recombination inside picoliter reactors===<br />
Manufacturing functionalized proteins in vitro poses a challenge, as it requires coordinated molecular assemblies and multi-step reactions. In this project we aim to control, over time and space, the production of proteins tagged with a silver-binding peptide for in situ silver nanoparticle nucleation inside microdroplets generated by microfluidic devices. Combining a transcription-translation system with protein coding genes and a recombination logic inside microdroplets provides spatial control. Moreover, in the microfluidic chamber we can pinpoint the beginning of synthesis, and easily track and isolate the droplets. Site-specific recombination generates a molecular timer for temporal control of protein synthesis. Unlike transcriptional regulation, this method gives true all-or-none induction due to covalent modification of DNA by Flp recombinase. Determining the transfer curve of inter-FRT site distance versus average recombination time allows the onset of gene expression to be predicted. We then apply this Flp reporter system as a powerful PoPS measurement device.<br />
<br />
===[[Team:Bologna | Team Bologna:]] T-REX: Trans-Repression of Expression. A BioBrick gene-independent control of translation===<br />
The project aims to realize a device with standard biological parts for the post-transcriptional control of gene expression, regardless of the gene sequence to be silenced. We designed the T-REX device, composed of two non-coding DNA sequences: the TRANS-repressor and the CIS-repressing parts. TRANS-repressor acts as a silencer of CIS-repressing RNA target. This target includes a region complementary to the TRANS-repressor sequence antisense, ends with RBS, and is assembled upstream of the coding sequence to be silenced. Upon binding of TRANS-repressor and CIS-repressing RNAs, the access to RBS by ribosomes is hampered, silencing translation. Accordingly, the amount of TRANS-repressor controls the translation rate of the regulated gene. The TRANS-repressor sequence was determined by a computational analysis performed to minimize the interference with the genomic mRNAs and to maximize the base-pairing interaction to the CIS-repressing RNA. The T-REX device is proposed as a universal and fast switch in synthetic gene circuits.<br />
<br />
===[[Team:British_Columbia | Team British_Columbia:]] Development of a modular, analog E. coli biosensor===<br />
To date, efforts to design a whole-cell biosensor capable of detecting levels of one or more biological inputs and responding in an analog mode has been elusive. We have designed a system of synthetic constructs implemented in an E. coli chassis that will allow detection of continuously varying levels of a single metabolic input and report on the concentration with qualitative output depending on threshold levels of the input. Our system design utilizes RNA-level hairpin hybridization and antisense technologies linked to various reporters. Because our approach is modular and does not depend on either endogenous protein processing or exogenous RNA, we envision that such a system could find applications in many different fields, including environmental sensing, detection of diagnostic of therapeutic biomarkers, and systems biology. <br />
<br />
===[[Team:Brown | Team Brown:]] Engineering Staphylococcus Epidermidis to Secrete Recombinant Histamine Binding Protein in Response to Changing Histamine Concentration===<br />
The 2009 Brown iGEM Team aims to treat allergic rhinitis (hay fever) by engineering Staphylococcus epidermidis to secrete a histamine-binding protein, rEV131, in response to elevated histamine concentrations during an allergic attack. rEV131 was cloned from a species of tick, Rhipicephalus appendiculatus. We are putting the rEV131 gene into an endogenous element of human nasal flora, Staphylococcus epidermidis. rEV131 will have a secretion tag specific for S. epidermidis. To synchronize rEV131 production with elevation of histamine concentration, we are computationally designing a novel histamine receptor. This histamine-responsive receptor will induce expression of rEV131. Although S. epidermidis is a non-pathogenic species, when it reaches a certain population threshold it produces potentially hazardous biofilms. To mitigate this concern, we have engineered safety measures that prevent excessive growth by repurposing S. epidmidis’ natural population sensor to cue each cell’s "suicide" when a population has reached a dangerous size.<br />
<br />
===[[Team:Calgary | Team Calgary:]] Reprogramming a Language and a Community===<br />
iGEM Calgary contributed a second quorum sensing (QS) system to the Registry. The Vibrio harveyi AI-2 QS signalling system has been engineered in Escherichia coli . Coupled with quorum quenching, our system allows us to target biofilm maintenance. The robustness of AI-2 signalling in E. coli was characterized in the lab and compared to data from mathematical models of the system built using the Matlab Simbiology toolbox and the emerging Membrane Computing framework in Mathematica. We also undertook community outreach projects in order to enhance the synthetic biology community. Specifically, the Second Life platform was used to create an educational tool to train future synthetic biologists in an accessible, user-friendly, virtual environment. Moreover, we examined the implications of our project in light of the recently proposed proactionary and precautionary frameworks with special focus on ethical, environmental, economic, legal and social (E3LS) impact.<br />
<br />
===[[Team:Cambridge | Team Cambridge:]] E. Chromi: Triggering Pigment Production in E. Coli===<br />
Previous iGEM teams have focused on genetically engineering bacterial biosensors by enabling bacteria to respond to novel inputs, especially biologically significant compounds. There is an unmistakable need to also develop devices that can 1) manipulate the input by changing the behaviour of the response of the input-sensitive promoter, and that can 2) report a response using clear, user-friendly outputs. The most popular output is the expression of a fluorescent protein, detectable using fluorescence microscopy. But, what if we could simply see the output with our own eyes? The Cambridge 2009 iGEM team is engineering E. coli to produce different pigments in response to different concentrations of an inducer.<br />
<br />
===[[Team:CBNU-Korea | Team CBNU-Korea:]] Essarker: An Essential Remarker for a Minimal, Synthetic Genome===<br />
It is challengeable to create a synthetic genome for fulfilling the needs of energy and food. Without the assistance of computing tools, moreover, it would be much more difficult to make the synthetic genome. We here propose a key tool to help the creation of a genome as the essential step. The goal of Essarker is to help users design a minimal genome synthesized through the fundamental frame comprising the essential genes of replication. Essarker is a standalone software to manage and retrieve required sequences of genomes, and explore the essential gene order and direction and the related orthologous genes. It also identifies and visualizes the positions and orientations of genes. In addition, it shows optimal ordering of essential genes and orthologs by statistical analysis.<br />
<br />
===[[Team:Chiba | Team Chiba:]] E. coli Time Manager Since 2008===<br />
Since 2008, we have been constructing the bacteria timer that "work together". The mechanism is very simple; (1) the "Transmitter cells" generates the signal molecules, whose concentration gradually increases, (2) when it reaches a certain level, the "Receiver cells" switch on the expression of any given genes. Precise control of the time of delay of this entire process, one can pre-set the time of expression of genetic functions in a predicable manner. By using Asyl-Homoserine Lactones(AHLs) that can freely pass through the cell membrane as signal molecules, the time can be shared, in real time, by all cells within the pot. This way, receiver (timer) cells would take the action all at once in right timing, minimizing the distribution in each cell's response time. This year, we are trying to make a platform for generating an animated pictures using series of new timer cells we have constructed.<br />
<br />
===[[Team:CityColSanFrancisco | Team CityColSanFrancisco:]]===<br />
We at CCSF have begun constructing a bacterial powered battery. The design has been generated with sustainability in mind, and aims to create an alternative to traditional fossil fuel technologies. The battery owes its capabilities to two strains of bacteria: the heterotroph Rhodoferax ferrireducens, and the photoautotroph Rhodopseudomonas palustris. Each strain will occupy its own concentration cell and after being cultured anaerobically, will either oxidize (in the case of R. palustris) ferris iron or reduce (in the case of R. ferrireducens) ferric iron. The resulting current will be collected and used to demonstrate the functionality of the battery. The reduction and oxidation reaction will be self-substaining. This process is further aided by the genetic modification of R. palustris. As a photosynthetic prokaryote, R. palustris generates glucose readily. We intend to share this glucose with R. ferrireducens by inserting a passive glucose transporter into the cells of R. palustris.<br />
<br />
===[[Team:Cornell | Team Cornell:]] Engineering the Bacillus Subtilis Metal Ion Homeostasis System to Serve as a Cadmium Responsive Biosensor===<br />
The goal of our project is to create a whole cell cadmium biosensor by attaching cadmium responsive promoters in Bacillus subtilis to fluorescent reporter proteins. Cadmium is a toxic heavy metal which has no known biological function. Ingestion of cadmium contaminated water can induce bone fractures and severe renal damage. Major sources of cadmium contamination include fertilizers, sewage sludge, manure and atmospheric deposition. Cadmium contaminated sewage is often used for irrigation purposes in many parts of the world, especially in developing nations. Crops grown in these contaminated soils are then sold in markets without any detoxification treatment. Current analytical methods such as atomic absorption spectroscopy, though highly sensitive, are significantly more expensive than bacterial biosensors and are unable to measure the amount of bioavailable cadmium.<br />
<br />
===[[Team:DTU_Denmark | Team DTU_Denmark:]] The redoxilator, and the USER fusion assembly standard===<br />
The Redoxilator: By in silico design and computer modelling followed by gene synthesis, we have constructed a molecular NAD/NADH ratio sensing system in Saccharomyces cerevisiae. The sensor works as an inducible transcription factor being active only at certain levels of the NAD/NADH ratios. By the coupling of a yeast optimized fast degradable GFP, the system can be used for in vivo monitoring of NAD/NADH redox poise. A future novel application of the system is heterologous redox coupled protein production in yeast. The USER fusion standard: Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER(TradeMark) cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any ‘scars’ from the restriction enzyme digestions.<br />
<br />
===[[Team:Duke | Team Duke:]] One-Step Construction of a Bioplastic Production Pathway in E. coli===<br />
A convenient ligation-free, sequence-independent one-step plasmid assembly and cloning method is developed [Quan J, Tian J (2009) Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways. PLoS ONE 4(7): e6441]. The strategy, called Circular Polymerase Assembly Cloning (CPEC), relies on polymerase extension to assemble and clone multiple fragments into any vector. Using this method, we are able to quickly assemble a metabolic pathway consisting of multiple enzymes and regulatory elements for the production of a biocompatible as well as biodegradable plastic polymer in E. coli.<br />
<br />
===[[Team:Edinburgh | Team Edinburgh:]] Defusing a dangerous world: a biological method for detection of landmines===<br />
Landmines left over from past conflicts are a major hazard in the world, killing and maiming many people every year. We have sought to engineer a bacterium able to detect TNT and its degradation products, nitrites, in the environment. Our system is based around a previously published computationally designed TNT-sensing protein derived from the periplasmic ribose binding protein, which interacts with an EnvZ-Trg transmembrane hybrid fusion protein and a nitrite-responsive repressor to trigger a pathway of TNT degradation and visualization using combined output from a bacterial luciferase and Yellow Fluorescent Protein. We envisage that the detection system could be applied by spraying the organism on soil where the presence of landmines is suspected, and detecting luminescence using low-light sensing. Once located, the mines could be safely removed. This system could be extended to detect other analytes in the environment.<br />
<br />
===[[Team:EPF-Lausanne | Team EPF-Lausanne:]] E. Colight===<br />
Recent discoveries of photoreceptors in many organisms have given us insights into the interest of using light-responsive genetic tools in synthetic biology. The final goal of our project is to induce a change in gene expression, more specifically to turn a gene on or off, in a living organism, in response to a light stimulus. For this we use light-sensitive DNA binding proteins (or light-sensitive proteins that activate DNA binding proteins) to convert a light input into a chosen output, for example fluorescence, through a reporter gene such as RFP. Demonstrating that the light-induced gene switch tool works in vivo would show that easier and faster tools can potentially be made available in several fields of biology, as such tools can induce more localized, more precise (time resolution and reversibility) and drastically faster genetic changes than currently used ones, thus allowing research to evolve even better.<br />
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===[[Team:Freiburg_bioware | Team Freiburg_bioware:]] Universal endonuclease – cutting edge technology===<br />
Gene technology is driven by the use of restriction endonucleases. Yet, constraints of limited sequence length and variation recognized by available restriction enzymes pose a major roadblock for synthetic biology. We developed the basis for universal restriction enzymes, primarily for routine cloning but also with potential for in-vivo applications. We use a nucleotide cleavage domain fused to a binding domain, which recognizes a programmable adapter that mediates DNA binding and thus cleavage. As adapter we use readily available modified oligonucleotides, as binding domain anticalins, and as cleavage domain FokI moieties engineered for heterodimerization and activity. For application, this universal enzyme has merely to be mixed with the sequence-specific oligonucleotide and the target DNA. Binding and release are addressed by thermocycling. We provide concepts for in-vivo applications by external adapter delivery and activity regulation by photo switching. Additionally, an argonaute protein is engineered towards a DNA endonuclease.<br />
<br />
===[[Team:Freiburg_software | Team Freiburg_software:]] SynBioWave – A Collaborative Synthetic Biology Software Suite===<br />
Synthetic Biology, which aims at constructing whole new genomes, is pushed forward by many users and relies on the assembly of genetic elements to devices and later systems. The construction process needs to be transparent and even at final stages control at the basepair level is required. We are building a software environment enabling multiple distributed users to analyze and construct genetic parts and ultimately genomes with real-time communication. Our current version demonstrates the principle use as well as the power of the underlying Google Wave protocol for collaborative synthetic biology efforts. Many wave-robots with a manageable set of capabilities will divide and conquer the complex task of creating a genome in silico. The initial developments of 'SynBioWave' lay the ground for basic layout, calling and data exchange of wave-robots in a clear and open process, so that future robots can be added and shared easily<br />
<br />
===[[Team:Gaston_Day_School | Team Gaston_Day_School:]] Development of a Red Fluorescent Nitrate Detector===<br />
Increasing levels of fertilizer required for mechanized farming can result in elevated nitrate levels in soil and groundwater. Due to contaminated food and water, humans are at risk for Methemoglobinemia caused by enterohepatic metabolism of nitrates into ammonia. This process also oxidizes the iron in hemoglobin, rendering it unable to carry oxygen. Infants in particular are susceptible to Methemoglobinemia, also known as “Blue Baby Syndrome”, when formula is reconstituted using contaminated water. In order to prevent Methemoglobinemia, it is essential to detect high concentrations of nitrates. Fnr-NarG is an aerobic mutation of the nitrogen-sensitive promoter NarG that was provided by Dr. Lindow at UC Berkeley. By combining Red Fluorescent Protein with an aerobic mutant strain of NarG, the creation of Red Fluorescent Nitrate Detector (RFND) is possible. RFND is economically efficient because of its ability to self-replicate.<br />
<br />
===[[Team:Groningen | Team Groningen:]] Heavy metal scavengers with a vertical gas drive===<br />
Heavy metal pollution of water and sediment endangers human health and the environment. To battle this problem, a purification strategy was developed in which arsenic, zinc or copper are removed from metal-polluted water and sediment. In this approach Escherichia coli bacteria accumulate metal ions from solutions, after which they produce gas vesicles and start floating. This biological device encompasses two integrated systems: one for metal accumulation, the other for metal-induced buoyancy. The uptake and storage system consists of a metal transporter and metallothioneins (metal binding proteins). The buoyancy system is made up of a metal-induced promoter upstream of a gas vesicle gene cluster. This device can be changed to scavenge for any compound by altering the accumulation and the induction modules. The combination of both systems enables the efficient decontamination of polluted water and sediment in a biological manner. <br />
<br />
===[[Team:Harvard | Team Harvard:]] Interspecies Optical Communication Between Bacteria and Yeast===<br />
Optical communication is central to interactions between many multicellular organisms. However, it is virtually unknown between unicellular organisms, much less between unicellular organisms of different kingdoms of life. Our team has constructed a system that allows for interspecies, bacteria-to-yeast optical communication. To permit bacteria to send an optical signal, we expressed in E. coli a red firefly luciferase under IPTG induction. In yeast, we used a yeast-two-hybrid-system based on the interaction between the red-light-sensitive Arabidopsis thaliana phytochrome PhyB and its interacting factor PIF3. Interaction between PhyB and PIF3 is induced by the red light from the bacteria, resulting in transcription of the lacZ gene. This is an excellent demonstration of the principles and potential of synthetic biology: this system not only allows for interspecies optical communication, but enables us to optically bridge a physically separated canonical lac operon using light as a trans-acting factor.<br />
<br />
===[[Team:Heidelberg | Team Heidelberg:]] Spybricks - a starter kit for synthetic biology in mammalian cells===<br />
Mammalian synthetic biology has a huge potential, but it is in need of new standards and a systematic construction of comprehensive part libraries. Promoters are the fundamental elements of every synthetic biological system. We have developed and successfully applied two novel, in silico guided methods for the rational construction of synthetic promoters which respond only to predefined transcription factors. Thus, we have been able to create a library of promoters of different strength and inducibility. To characterize the promoters, we have developed standardized protocols for comparable measurements of promoter strength by either transient or stable transfection. These synthetic promoters can be used as “spybricks” which enable the construction of assays for simultaneously monitoring several pathways in a cell. However, the potential of synthetic promoters goes far beyond this application: e.g. in virotherapy, these promoters could be used for selective gene expression in target cells.<br />
<br />
===[[Team:HKU-HKBU | Team HKU-HKBU:]] Biomotor===<br />
Much hope has been laid on nanorobots in their application in therapeutics in this era of catheters and minimally invasive surgery, but the problem remains that purely mechanical nanorobots lack sufficient locomotive power to perform their intended tasks. Our 'bio-motor' aims to breach this gap to bring a foundational advancement. In our model, Escherichia coli cells are engineered to specifically express streptavidin at pole(s), which allows cells to adhere in the same orientation to a microrotary motor through biotin-streptavidin interaction. Thus, with the propulsion generated by bacterial flagella, this synthetic device is capable to convert biological energy into mechanical work. Furthermore, the propulsion energy was programmed to be adjustable by controlling E.coli swimming speed, i.e. putting E.coli cheZ gene under the control of ptet. This technology has tremendous potential to be applied in various fields including biomedicine, bio-energy, and bioengineering.<br />
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===[[Team:HKUST | Team HKUST:]] SynBiological Bug Buster===<br />
We aim to engineer a novel yeast strain that can detect, attract and eliminate pests. This strain would serve as an environmental-friendly substitute for pesticides. The idea is demonstrated by constructing an odorant sensing module, coupled production of chemical attractant and production of pest-killing binary toxin in yeast to kill pests lured to the yeast culture. A chimera G-protein coupled receptor (GPCR) responsive to an odorant chemical is coupled to the yeast mating pathway that can be activated upon ligand binding. It leads to over-expression of an endogenous yeast transaminase that catalyzes a reaction to yield 2-phenylethanol. Constitutively expressed binary toxin in the yeasts would poison the attracted pests after their consumption, as tested by feeding drosophilae. In addition to being a pesticide substitute, this cheaply-maintained engineered yeast strain also serves as a research reagent to screen for GPCRs that bind to certain ligands.<br />
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===[[Team:IBB_Pune | Team IBB_Pune:]] Constructing multi-strain computational modules using Nucleotide and Protein mediated cell-cell signaling.===<br />
Building complex genetic circuits in a single cell becomes difficult due to the formidable task of co-transforming large nucleotide sequences in addition to the imposed metabolic burden on the cell. Can a complex system be divided into independent modules that reside in different cells and interact with each other using nucleotide and protein mediated cell-cell signalling to act as a single unit? We seek to address this problem using a three pronged approach. Firstly, we are trying to introduce natural competance genes into the biobrick framework which will act as nucleotide importers. We are also building a protein export system using the TAT dependent export pathway. Finally, we are attempting to construct a multi-state turing machine which is a compound, modular computational system that has independent, interacting states which applies the above principle. We hope that this approach overcomes the obstacles in building more complex and composite circuits.<br />
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===[[Team:IIT_Bombay_India | Team IIT_Bombay_India:]] Analysis of multiple feedback loops using Synthetic Biology===<br />
One of the major objectives of synthetic biology is to unveil the inherent design principles prevailing in biological circuits. Multiple feedback loops (having both positive and negative regulation) are highly prevalent in biological systems. The relevance of such a design in biological systems is unclear. Our team will use synthetic biology approaches to answer these questions. Our team comprises of nine undergraduates, 3 graduate students as student mentor and two faculty mentors, one each from biology and engineering background. The project specifically deals with the analysis of effect of single and multiple feedback loops on gene expression. This project will involve theoretical and experimental studies. We have designed synthetic constructs to mimic multiple feedbacks. The focus of our experimental work will be to visualize the effect of multiple feedback loops on the synthetic construct using single cell analysis. The project will provide insights into the roles of multiple feedback loops in biological systems.<br />
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===[[Team:IIT_Madras | Team IIT_Madras:]] PLASMID: Plasmid Locking Assembly for Sustaining Multiple Inserted DNA===<br />
Any episome introduced into the cell shows segregational asymmetry accompanied with differential growth rates in the absence and presence of episome leading to an overall loss of the episomal unit in the absence of any selective pressure. We have designed a versatile system which maintains any given plasmid DNA in E.coli by using user-defined selection pressures, limited only by the presence of a response element to said pressure, like most antibiotics, certain chemicals and physical conditions. Depending on this selection pressure, a custom plasmid retaining system can be designed and co-transformed with the plasmid of interest to maintain it. A similar system can be used to “lock” the function of a gene of interest, like a combination lock, which is unlocked only when the cultures are grown in a pre-determined order of selection pressures. In principle, using this locking system, multiple plasmids can be maintained using a single selection pressure. <br />
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===[[Team:Illinois | Team Illinois:]] Bacterial Decoder===<br />
The Illinois iGEM team has been working to engineer a decoder function within E. coli. Decoders are logic devices used frequently in low-level computer architecture. We are creating a 2 to 4 decoder, which takes two binary inputs to activate one of four outputs. Each output corresponds to a specific combination of the inputs. With the presence of lactose and arabinose, our Bacterial Decoder will express Green Fluorescent Protein. If only lactose is present, a different fluorescent protein will be expressed. This goes for the other two combinations as well (only arabinose, or neither sugars). To implement logic we use combinations of small non-coding RNAs and transcription factors. The system allows the next engineer to swap standard parts in and out to change the inputs and outputs. Our Bacterial Decoder can help sense for multiple environmental cues, having implications for medical diagnostics and environmental and water contaminant detection.<br />
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===[[Team:Illinois-Tools | Team Illinois-Tools:]] Interactive Metabolic Pathway Tools===<br />
Interactive Metabolic Pathway Tools (IMP Tools) is an open source, web based program that involves model-guided cellular engineering where new metabolic functions can be added to existing microorganisms. This program will assist in the design stage of synthetic biology research. IMP tools is written primarily in python using the Django web framework. It takes a user-defined input compound, output compound, and weighting scheme and determines the ideal pathway from the starting to the ending compound. Our program presents an exciting capability to help transform important processes in the world for applications ranging from bioremediation to biofuels.<br />
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===[[Team:Imperial College London | Team Imperial College London:]] The E.ncapsulator===<br />
For iGEM 2009 the Imperial College London team present you with The E.ncapsulator; a versatile manufacture and delivery platform by which therapeutics can be reliably targeted to the intestine. Our E.coli chassis progresses through a series of defined stages culminating in the production of a safe, inanimate pill. This sequential process involves drug production, self-encapsulation in a protective coating and genome deletion. The temporal transition through each of these stages has been individually optimised by both media and temperature. The E.ncapsulator provides an innovative method to deliver any biologically synthesisable compound and bypasses the need for expensive storage, packaging and purification processes. The E.ncapsulator is an attractive candidate for commercial pill development and demonstrates the massive manufacturing potential in Synthetic Biology.<br />
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===[[Team:IPN-UNAM-Mexico | Team IPN-UNAM-Mexico:]] Turing meets synthetic biology: self-emerging patterns in an activator-inhibitor network.===<br />
We present a synthetic network that emulates an activator-inhibitor system. Our goal is to show that spatio-temporal structures can be generated by the behavior of a genetic regulatory network. We implement the model by means of several biobricks. We construct a self activating module and correspondingly an inhibitory one. Self-activation dynamics is given by the las operon, while the inhibitory part is provided by the lux operon. Quorum sensing and diffusion of AHL provide the reaction-diffusion mechanism responsible for the formation of Turing patterns. The importance of our work relies on the fact that we show that the action of the morphogenes as originally proposed by Turing is equivalent to the effect of diffusion of chemicals interacting with the synthetic network, which accounts for the reactive part, a possibility implicit in Turing’s original work in the context of morphogenesis of biological patterns.<br />
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===[[Team:IPOC1-Colombia | Team IPOC1-Colombia:]] Molecular Device to Detect Sea Salinity===<br />
Different gene parts are being assembled in order to construct a device that is able to detect different salinity levels in the sea. The device is tested against different concentrations of sodium chloride, fluoride, calcium, and magnesium. Different parameters, such as reporter fluorescence, DNA concentration, growth of bacterial device will be used to measure the efficacy of the device. Computational modeling will be used in the project to complement the laboratory work. Importance of project: Colombia borders two oceans: the Atlantic and the Pacific.<br />
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===[[Team:IPOC2-Colombia | Team IPOC2-Colombia:]] Molecular Device that Biodegrades Pesticides===<br />
Different gene parts are being assembled, in order to construct a device that is able to mineralize and biodegrade recalcitrant pesticides. The device will be tested against different concentrations of different recalcitrant pesticides. Specific chassis will be assembled with gene parts from different metabolic pathways in order to finally reach mineralization of the pesticide. Different parameters, such as DNA concentration, ATP concetration, fluorescence of reporters, growth of bacterial device, and reduction of pesticide concentration, will be used to assess the efficacy of the device. Computational modeling will be used in order to complement the laboratory work.<br />
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===[[Team:Johns_Hopkins-BAG | Team Johns_Hopkins-BAG:]] Synthetic yeast genome Sc2.0 and Build-A-Genome===<br />
The JHU team will present the work of the Build-A-Genome course, powering the fabrication of synthetic yeast genome Sc2.0. Build-A-Genome provides students tools to meld seamless arrays of DNA into predesigned synthetic chromosomes. Our team develops new technologies for synthetic genomic fabrication. We developed a new standard, the Building Block, allowing production of much longer DNA sequences that can encode for more complicated cellular operations than allowed by current iGEM biobrick standards, as well as more standard iGEM-y devices. Through multiple rounds of homologous recombination we can create chromosome segments and eventually full chromosomes. We will present our improved methodology for building block synthesis, the software we have created to aid in our synthesis, applications of the yeast genome redesign and the new standard we have created. We will emphasize the Build-A-Genome course and its implications on future genomic technologies that both rely on and teach students.<br />
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===[[Team:KU_Seoul | Team KU_Seoul:]] Integrated Heavy Metal Detection System ===<br />
Our team project is designing synthetic modules for simultaneous detection of multiple heavy metals such as arsenic, zinc, and cadmium in E. coli. The ultimate goal is to build a micromachine sensing and determining of the concentration of heavy metals in a sample solution (e.g. the waste water). In order to design the system, we will employ two fluorescence proteins (GFP and RFP) and aryl acylamidase as signal reporters. Since each heavy metal promoter produces unique fluorescence or color by those reporters, if more than two heavy metals coexist in a solution, the results would be interpreted from the convoluted fluorescence and/or color rather than a single signal detection. The successful construction of the synthetic modules in E. coli can be utilized in the form of a lyophilized powder, which can be stored in a drug capsule to make it portable.<br />
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===[[Team:KULeuven | Team KULeuven:]] Essencia coli, the fragrance factory===<br />
'Essencia coli' is a vanillin producing bacterium equipped with a control system that keeps the concentration of vanillin at a constant level. The showpiece of the project is the feedback mechanism. Vanillin synthesis is initiated by irradiation with blue light. The preferred concentration can be modulated using the intensity of that light. At the same time the bacterium measures the amount of vanillin outside the cell and controls its production to maintain the set point. The designed system is universal in nature and has therefore potential benefits in different areas. The concept can easily be applied to other flavours and odours. In fact, any application that requires a constant concentration of a molecular substance is possible.<br />
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===[[Team:Kyoto | Team Kyoto:]] Time Bomb & Cells in cells===<br />
We have two projects. The first is “GSDD”, the project to make a "time bomb"---a unique system to control the time of cell death. We create timer mechanism by taking advantage of the end-replication problem and the protecting effect of lacI (bound to each end of DNA) against exonuclease digestion. As the cell divides, due to the end-replication problem, the "timer" DNA gets shorter, and eventually, the repressor expression level falls. Then the downstream killer gene becomes expressed. The other one, “Cells in Cells” is the project to make a cell. We defined making cells as making liposomes that can divide like mitochondria do. To approach our goal, we set two subgoals. One is to enable cells to take in liposomes. The other is to enable the liposomes to import proteins needed for mitochondrial division. We suppose this could be the first step to create artificial cells.<br />
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===[[Team:LCG-UNAM-Mexico | Team LCG-UNAM-Mexico:]] Fight fire with fire: phage mediated bacterial bite back===<br />
Bacteriophage infection represents a common matter in science and industry. We propose to contend with such infections at a population level by triggering a defense system delivered by an engineered P4 phage. P4 is a satellite of P2 phage, so it cannot assembly unless some P2 genes are present. Those indispensable genes will be expressed by an E.Coli strain, hence creating a production line of a P4 which will be able to deliver (transduce) standardized synthetic systems to E. Coli and possibly similar species. The defense system will consist of toxins for DNA and rRNA degradation, transcribed by T3 or T7 RNA-Polymerases, fast enough to stop phage's assembly and scattering. The system includes the spread of an AHL, hence "warning" the population to prepare against further T3 or T7 infection. We will implement a stochastic population model to simulate the infection processes and quantify the efficiency of our system.<br />
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===[[Team:Lethbridge | Team Lethbridge:]] A Synthetic Future: Microcompartments, Nanoparticles and the BioBattery===<br />
The issues surrounding energy production are becoming more prominent with increasing environmental concerns and the rising cost of energy. Microbial fuel cells (MFCs) use biological systems to produce an electrical current. Cyanobacteria are organisms which have been studied in MFCs and have been found to create a current, although not highly efficient (Tsujimura et al., 2001). We wish to increase the efficiency of the cyanobacteria MFC by introducing microcompartments to create a BioBattery. The microcompartments are created by the production of the protein lumazine synthase forms icosahedral capsids. As a proof of principle we will create this system within Escherichia coli and target two different fluorescent proteins within the microcompartment to observe fluorescence resonance energy transfer. Furthermore, we will be exploring a novel method for the mass production of uniform nanoparticles, which is more efficient and cost effective than current methods.<br />
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===[[Team:McGill | Team McGill:]] Activation‐inactivation signaling in one‐and two‐dimensions===<br />
Intercellular signaling constitutes the foundation of may disparate research fields such as neurophysiology, embryology, cancer research, and several others. We investigated a simple representational intercellular signaling network where a population of cells synthesizes and secretes an activator molecule capable of activation a second population of cells into synthesizing and secreting an inhibitor molecular which feeds back and inhibits the production of the activating molecule. This is known as an activation-inhibition system. We began by using a partial differential equation model of the system to explore the effect of varying the separation distance of the two populations of cells. We found that three types of dynamics were present: steady states, periodic oscillations, and quasi-periodic oscillations. We further designed two strains of E. coli capable of interacting with each other as an activation-inhibition system and endeavored to validate our modeling results in a biological system.<br />
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===[[Team:METU-Gene | Team METU-Gene:]] A Fast Healing Mechanism; Wound Dressing===<br />
In case of bulk loss of tissue or non-healing wounds such as burns, trauma, diabetic, decubitus and venous stasis ulcers, a proper wound dressing is needed to cover the wound area, protect the damaged tissue, and if possible to activate the cell proliferation and stimulate the healing process. By this purpose, designing a wound dressing which is natural, non-toxic, and biodegradable and imitating the actual wound healing mechanism which is forming on open wounds in mammalian tissues is our main purpose.By this wound covering, we will fasten the healing process, and protect the wounded area from infectious agents. In this wound dressing, there will be 4 layers including polyurethane layers and our bacteria colonies. Our bacteria colonies will be capable of synthesizing human epidermal growth factor and keratinocyte growth factor. The communication between these bacteria colonies will be dependent on quorum sensing molecules.<br />
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===[[Team:Michigan | Team Michigan:]] The Toluene Terminator===<br />
Toluene is a toxic substance used in petrol, paint, paint-thinners and adhesives. Through spills and improper disposal, toluene can contaminate soil and ground water environments. Using microorganisms to clean up toluene-contaminated sites can be an effective and economical way of degrading the pollution before it can spread throughout the environment. There is concern, however, that these non-native microorganisms may upset the balance of the ecosystem through unnatural competition or horizontal synthetic gene transfer. We are engineering the Toluene Terminator as a way to neutralize toluene pollution while addressing these concerns. It will have the capabilities of sensing and mineralizing the toluene into carbon dioxide and water, but this terminator will not be back. The Toluene Terminator will have a suicide mechanism which kills the bacteria in the absence of toluene.<br />
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===[[Team:Minnesota | Team Minnesota:]] Computational synthetic biology: How the Synthetic Biology Software Suite can guide wet-lab experiments===<br />
Synthetic biology has all the characteristic features of an engineering discipline: applying technical and scientific knowledge to design and implement devices, systems, and processes that safely realize a desired objective. Mathematical modeling has always been an important component of engineering disciplines: models and computer simulations can quickly provide a clear picture of how different components influence the behavior of the whole, reaching objectives quickly. Our presentation focuses on sophisticated mathematical models of synthetic biological systems that connect the targeted biological phenotype to the DNA sequence. The activities for iGEM 2009 included the development and testing of simulation tools that connect multiple levels of organization from molecules and their interactions, to gene regulatory relations, to emerging logical architectures in bacteria. We connected out tools to the Registry and validated the simulations with a significant experimental component, constructing and testing these synthetic biological systems in Escherichia coli.<br />
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===[[Team:Missouri_Miners | Team Missouri_Miners:]] A Synthetic Biology Apporach to Microbial Fuel Cell Development Utilizing E. Coli===<br />
Optimization of electron shuffle to external surfaces such as anodes was a primary goal. Geobacter sulfurreducens happened to be our model bacteria due to its ability in nature to efficiently export electrons extracelluarly. E. coli was the chassis for this experiment due to its well documentation and the fact that its genome already containing some key proteins in our preferred pathway. The proteins, such as extracellular pilin, MacA, and many other cytochromes, which E. coli does not have were isolated from Geobacter sulfurreducens and introduced into E. coli to formulate the most optimal pathway for generating electromotive force in a microbial fuel cell apparatus. Some problems were faced concerning plasmid engineering and the simple fact that Geobacter is anaerobic and E. coli is aerobic. The current work includes production and optimization of a microbial fuel cell into which our modified bacteria will be placed.<br />
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===[[Team:MIT | Team MIT:]] Photolocalizer===<br />
There has been growing interest in designing fast and reversible switchable controls over all steps of gene expression, from transcription to post-translational modification. Our project involves engineering S. cerevisiae to localize proteins to various points in the cell in response to light exposure. Under red light, a tagged protein of interest localizes to a specific target, while exposure to far-red light causes the protein to rapidly delocalize and diffuse throughout the cell. This is accomplished using the PhyB-PIF3 system, a light-based transcriptional regulation system found in Arabidopsis. This project has two components. 1) Metabolically engineering yeast to endogenously produce PCB, a tetrapyrrole necessary for system activation, and 2) adapting the PhyB-PIF3 system to localize proteins of interest to different targets in the cell. The versatility and applications for this system are vast, ranging from cellular diffusion studies to easily synchronizing cell division for entire populations.<br />
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===[[Team:MoWestern_Davidson | Team MoWestern_Davidson:]] Rolling Clones: Can’t get no SATisfaction===<br />
Our team goal was to advance the developing field of bacterial computing. The Satisfiability (SAT) problem was the first mathematical problem proven to be NP-complete. A SAT problem is formed by connecting true-false variables with OR to form clauses and connecting clauses with AND. The goal is to determine if true-false values can be assigned to each variable to make the overall logical expression true. Our designed system uses frameshift suppressor tRNAs as inputs and frameshift suppressor leaders (FSLs) that process the inputs to enable the translation of fluorescent proteins exactly when an appropriate combination of inputs is present. The results illustrate the potential of engineered living cells to evaluate challenging mathematical problems. Our project also explored two aspects of synthetic biology education: a survey and analysis of public opinion and teachers’ knowledge of synthetic biology and the design and construction of physical models of a frameshift suppressor. <br />
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===[[Team:NCTU_Formosa | Team NCTU_Formosa:]] Bacterial referee with the adjustable timer and counter functions===<br />
Our team constructed a controllable synthetic genetic circuit in Escherichia coli which has timer and counter functions. The circuit works as an OR gate to integrate temporal and environmental signals. The output (red fluorescent protein: RFP) of the OR gate is ON when one of the input signals is ON. The timer function is controlled by Lac promoter, and the concentration of lactose determines timer’s working length. After added lactose is consumed by E. coli, the RFP will be translated to remind us that time’s up. The counter function can detect the bacteria population with LuxI/LuxR device; moreover, the counter sensitivity is controlled by the strength of TetR repressible promoter. If external bacteria invade the system, the extra AHL produced by them will induce the RFP translation to warn us the contamination. Our project can be applied to storage warning signs of fresh food, contact lens, and wound dressing.<br />
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===[[Team:Nevada | Team Nevada:]] Cinnamicide: Producing a Natural Insecticide against Mosquito Larvae in E. coli and Duckweed===<br />
Cinnamaldehyde is a natural insecticide against mosquito larvae that shows low toxicity towards other organisms. The objective of this project is to engineer the cinnamaldehyde biosynthetic pathway into E. coli to develop an inexpensive and readily available source of this compound. By introducing the genes encoding phenylalanine ammonia lyase, cinnamate-CoA ligase, and cinnamoyl-CoA reductase, it should be possible to produce cinnamaldehyde from available phenylalanine in E. coli. Once we have proven that we can produce cinnamaldehyde in E. coli, we will engineer cinnamaldehyde production in duckweed, a small aquatic plant. Because mosquito larvae feed on duckweed detritus, the engineered plant will serve as an excellent vehicle to deliver cinnamaldehyde for mosquito control.<br />
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===[[Team:Newcastle | Team Newcastle:]] Bac-man: sequestering cadmium into Bacillus spores===<br />
Cadmium contamination can be a serious problem in countries where polluting industries are located close to agricultural sites. Our team developed a design to address this problem using the resiliant spore-forming bacterium Bacillus subtilis. We engineered B. subtilis to sense and sequester cadmium from the environment into metallothionein containing spores, rendering it bio-unavailable. Germination of the spores can be disabled, making retrieval of the cadmium unnecessary since they can persist intact for thousands of years. We computationally simulated the life cycles of individual cells and entire cell populations, to estimate the parameter values necessary to maintain sustainable populations of sporulating, germinating and vegetative cells. Our design required us to engineer stochastic differentiation processes at a single cell level. A sporulation rate tuner was developed and we also engineered a tuneable stochastic invertase switch to stochastically control cell differention and fate. <br />
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===[[Team:NTU-Singapore | Team NTU-Singapore:]] Plaque Out!===<br />
The NTU iGEM ’09 team is proud to be working on a proposed alternative treatment for atherosclerosis. Our system, pLaqUe Out!, ideally based in a macrophage chassis, when introduced into the bloodstream, will be activated by a symptom typical of plaque buildup. Upon activation, our system will release a cholesterol degrading enzyme, a novel reporter protein and a vasodilator. The cholesterol degrading enzyme will break down the plaque. The reporter protein was chosen for expression because of its unique fluorescent property. This allows the plaque site to be identified in a manner similar to X-ray visualization. Finally the vasodilator will simultaneously dilate the blood vessels for better flow, as well as switch off the extended activity of our system. In the interests of time, this system is first modeled using E.Coli.<br />
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===[[Team:NYMU-Taipei | Team NYMU-Taipei:]] ViroCatcher===<br />
1. The objective: Binding viruses to designer ViroCatcher cells that cannot support viral replication to diagnose, attenuate, and prevent infection. 2. What we intend to do: (1) Make our designer cell safe, (2) Express specific cell surface receptors and antibodies to catch the virus, (3) Transduce the signal after viruses attached for feedback control, and (4) Remove the viruses along with ViroCatcher itself. 3. Anticipated results: the ViroCatcher is made safe for the bloodstream. When it is injected into the bloodstream, our ViroCatcher passively lies around letting viruses attach to it by using its 4 receptors: CD4 (for HIV), Integrin (for various viruses), Sialic Acid (for Influenza), and Antibodies (for Influenza). After enough viruses attach to it, or after a certain amount of time elapses, it removes itself from the bloodstream by calling macrophages to eat it up.<br />
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===[[Team:Osaka | Team Osaka:]] ColorColi: Painting tools toward bio-art===<br />
Bio-art has appeared as a crossover of life science and art in 21st century. Such artworks can give rise to a number of issues and metaphors accompanying the advance of science and technology. Astonishingly, there are still few collaborations between bio-art and synthetic biology. In this context, we engineered Salmonella enterica cells to function as new painting tools in bio-art. Specifically, we try to program cells to automatically form various pattern and gradation of colors by sensing cell identity and density by means of quorum sensing. Moreover, we will extend these tools for actual paintings and artworks to consider the ethical implications such as ’view of life’. This collaborative project can show the social situation or question of life science.<br />
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===[[Team:Paris | Team Paris:]] Message in a Bubble: a robust inter-cellular communication system based on outer membrane vesicles.===<br />
Sending a message across the ocean… Outer membrane vesicles (OMV), naturally produced by gram negative bacteria as E. Coli, are strong candidates for long-distance messaging. Our engineered communication platform consists of controlling OMV production by destabilizing membrane integrity through over-expression of specific periplasmic proteins (e.g., TolR). In order to control and modulate message content, we used fusions with OmpA signal sequence and the ClyA hemolysin as delivery tags. A targeting system was developed, based on the outer-membrane expression of Jun/Fos leucine zippers to control the vesicle flux between donor and recipient cells. Once received, the signal from incoming vesicles is transduced through a modified Fec pathway, whereby the receptor is provided by the OMV. Computational models provided insight to all of the above steps. Such reliable communications systems have wide biotechnological implications, ranging from targeted drugs delivery and detoxification to advanced division of labor or even cell-based computing.<br />
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===[[Team:PKU_Beijing | Team PKU_Beijing:]] Conditioned Reflex Mimicking in E.coli===<br />
We are engineering our E. coli cells to process the correlation information of two enviornmental signal, similar to the process of conditioning in higher orgamisms. We have constructed and tested a series of AND gates which can sense the two signals: the conditioned and unconditioned stimuli. With the presence of both signals, the AND gate outputs a repressor protein and then changes the state of the bistable switch, which acts as a memory module. In this way, our E. coli cells can convert the information about the concurrence of the two signals into its memory. After the memory module is switched and given the "conditioned stimulus", the E. coli cells will pass the information to the reporter module and thus exhibit the "conditioned response."<br />
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===[[Team:Purdue | Team Purdue:]] Engineered Microglia to Locate CD133+ Tumor-Initiating Cells===<br />
Glioblastoma multiforme (GBM) is one of the most common forms of primary brain cancer, which usually results in fatality. To date, it has been difficult to overcome primary brain cancer resulting from GBM, primarily because the cancer-initiating cells are suspected to be highly resistant to current cancer therapies. Specifically, CD133+ cells have shown resistance to hypoxia, irradiation, and some forms of chemotherapy. CD133+ hunting machines will be created by genetically engineering microglial cells (BV-2) with mammalian expression vectors. The project will also take advantage of inherent qualities of the microglia such as constant environmental sensing and quick motility. The engineered BV-2s will be equipped to locate the specific GBMs and label the targeted cells with a tat-GFP fusion protein. It is the goal of this study to show an alternative approach to cancer treatment, and to emphasize the power of biologically available options to fight the disease.<br />
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===[[Team:Queens | Team Queens:]] Plaque Busters: A Synthetic Biology Approach to Targeted Drug Delivery Treatment of Atherosclerosis===<br />
Atherosclerosis is associated with the buildup of plaques in the vascular walls. Currently, treatment for atherosclerosis involves preventative measures and surgical removal of plaque, angioplasty, and stent placement. We sought to develop an E. coli chassis delivering anti-atherosclerotic substances to the site of plaque in vasculature. Inflamed endothelial cells express VCAM-1, a receptor normally binds to the leukocyte antigen VLA-4. We attempted to express a VLA-4 fragment in E. coli, in order to selectively attach the cells to plaques. In vitro binding test uses inflamed murine endothelial cells which express VCAM-1. Results are pending. Our bacterial chassis also carries several inducible “effector” systems which, upon binding, release substances that facilitate plaque stabilization and regression. Effector systems include heme oxygenase-1, serum amyloid A and atrial natriuretic peptide. Expression of HO-1 in E. coli has been confirmed using spectroscopy. Testing for SAA secretion and ANP-induced gene expression in endothelial cells is ongoing. <br />
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===[[Team:SDU-Denmark | Team SDU-Denmark:]] Bacto Bandage - Quorum-quenching S. Aureus Biofilm Formation, One Peptide at a Time===<br />
Our goal is to create an E. coli strain, which inhibits Staphylococcus aureus biofilm formation in wounds by producing RNA III-inhibiting-peptide (RIP). S. aureus is one of the largest causes of hospital infections, each year infecting millions of people around the globe. S. aureus is normally commensal, but can create biofilms on implanted medical devices and in post-operational wounds. Biofilm is increasingly hard to treat, as a result of growing resistance to many types of antibiotics. By manipulating E. coli to express a synthetic RIP peptide tagged with an export signal, we hope to reach this goal. RIP has been shown to hinder the quorum-sensing processes essential for biofilm development in S. aureus, thereby making it harder for the bacteria to cause infections. We propose making a bandage that contains our engineered bacteria behind a semipermeable membrane, allowing only small peptides such as RIP to pass through, into the wound. <br />
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===[[Team:Sheffield | Team Sheffield:]] E. Coli Switch===<br />
By modifying E.coli so that it can use a phytochrome- with a light receptor- from cyanobacteria as a trigger of protein generation. This pathway is controlled by a certain wavelength of red light, acting as a system switch for lacZ production. LacZ can react with substrate X-gal and form a blue precipitate as a reporter. However, other reporter genes can be attached to the lacZ gene, so different reporters can be expressed. From the fact that this mechanism is sensitive to a certain wavelength of light, we hope to create a system that can be sensitive to various wavelengths and hence triggering different protein generation. Through this the E.coli can become a wavelength sensor; a different wavelength can trigger a different production of protein, for example various types of fluorescent protein, giving a different a colour-scaled indication of the wavelength of the environment around the E.coli.<br />
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===[[Team:SJTU-BioX-Shanghai | Team SJTU-BioX-Shanghai:]] Hypnos' Curse: E.coli the napper===<br />
Inspired by the natural regulator of circadian bioclock exhibited in most eukaryotic organisms, our team has designed an E.coli-based genetic network derived from the toxin-antitoxin system (TA system). The relE protein(toxin), is an RNase that preferentially cleaves mRNA stop codons, severely inhibiting translation and preventing colony formation. Whereas expression of relB protein(antitoxin) and tmRNA forms a rescue system to reverse inhibitory effects. Based on these mechanisms our network functions as a bacterial bioclock oscillating between the two states of dormancy and activity. Potential applications of our project include lifespan prolongation of prokaryotes and eukaryotes, since the metabolic process of microbes is vastly decelerated during the dormancy state, just like that of bears and hedgehogs in their hibernation.<br />
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===[[Team:Slovenia | Team Slovenia:]] nanoBRICKsPRO – synthetic smart nanomaterials from nano to macro===<br />
Nanotechnology designs materials with advanced properties based on the control of structure at the nanoscale. Biological systems provide an attractive opportunity to design and easily manufacture material with programmable properties. DNA origami demonstrated the power of this technology by creating a variety of assemblies that can be easily encoded in the nucleotide sequence. However, for biological nanodevices nature favors polypeptides over nucleic acids due to stability and versatility of amino-acid side chains. With few exceptions protein and peptide assemblies have been considered too difficult for the bottom-up design due to complex interactions and manufacturing problems specific for each case. We present technology for manufacturing nanomaterials based on combinations of modular peptide elements and protein domains, which allow self-assembly into complex tertiary structures with designed macroscopic properties. We will demonstrate the feasibility and potentials of protein nanotechnology by design, streamlining the production and technological application of nanomaterials based on nanoBRICKsPRO. <br />
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===[[Team:Southampton | Team Southampton:]] E.colYMPIC GAMES===<br />
The project exploits quorum sensing in E. coli to engineer interactions between 'species' such that complex spatiotemporal patterns are generated. We have two systems that correspond the game ³Rock, Paper, Scissors² (RPS) and to Conway¹s ³Game of Life² (GoL). In GoL expression/diffusion of lactones is exploited to create local rules that modulate expression of a fluorescent protein. Fluorescence patterns for different combinations of conditions are modelled using a new simulation tool designed to be generically applicable to inter-bacterial communication. In the RPS system three 'species' each produce a different fluorescent protein whose expression is downregulated by a lactone from one of the other 'species' and hence the interaction network is intransitive. Simulations indicate that when this game is played out on culture plates, a range of complex patterns evolve with time. Also, selective patterning of the different 'species' allows for new 'racetrack' or 'playing field' type of interactivity.<br />
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===[[Team:Stanford | Team Stanford:]] Immuni-T. coli: A Probiotic Approach to Diagnosing and Treating Inflammatory Bowel Disease (IBD)===<br />
Homeostasis relies on the balance between immune cell types, disruption of which leads to autoimmunity. The Stanford team has applied synthetic biology to a longstanding objective of immunotherapy: restoration and maintenance of homeostasis. Stanford’s Escherichia coli-based probiotic will polarize T cell differentiation along antagonistic fates - immunosuppressive Treg and inflammatory Th17 phenotypes - in response to local conditions. Our device design consists of two parts: one that modulates deleterious Treg-driven immunosuppression and another that engages Th17-mediated inflammation. Through specific sensors and effectors, therapy will oscillate between dampening pathologic inflammation and immunosuppression until a balance in the local T cell population is achieved. Securing such homeostasis between these populations has therapeutic implications for autoimmune disorders like IBD, HIV and cancer. We envision our novel and directed probiotic therapy for IBD as acting at the interface between commensal bacteria and human lymphocytes, integrating cutting-edge immunology with synthetic biology.<br />
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===[[Team:SupBiotech-Paris | Team SupBiotech-Paris:]] Double vectorisation system (DVS)===<br />
As a part of the iGEM competition, we decided to develop a new process, allowing the protection of the active biological principle. This type of process exists already, and it is called a vector. It may be biological as viruses, or chemicals as polymeric nanoparticles. Whatsoever its nature, the vector encounters many problems : Stability, targeting, membrane passage, and the immune response. Therefore, we tried to achieve the ideal vector, being the most stable as possible, can easily penetrate its targets, and outwit the immune system. We have created a double vectorization system, by using jointly a bacteria and a phage. The first vector which is bacterial, will target the tissue, and resist to the immune system. The second vector is a phage, will be used for cell targeting and membrane penetration. The combination of the two systems improves the intrinsic abilities of vectors, and offers new possibilities for applications.<br />
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===[[Team:Sweden | Team Sweden:]] The Linguistic Cell: Sentence Parsing Bacteria===<br />
Language is an essential part of our civilization. But making sense out of a series of words can only be achieved by certain rules that underlie the language. This set of rules is called a grammar. A grammar tells us how to order words in a meaningful way. These rules can be implemented as a Finite State Automaton (FSA), which for every new word input moves from the current state to the next until it reaches the end of input. We propose in our project a biological model which is based on this con- cept of language parsing from computational linguistics.<br />
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===[[Team:Tianjin | Team Tianjin:]] Cyanobacteria convertor & Microcystins detector===<br />
Project 1: Inspired by several features of cyanobacteria, which is low-grade, fast-growing, photosynthetic and easy to operate. We aim to construct an pathway in cyanobacteria so that when it is carrying photosynthesis,carbon dioxide can be transferred into target production, ethanol. Project 2: This project is to design a Yeast Two-hybrid system aimed at Microcystins(MCs) detection in waters. The MCs detection device we design takes the advantage of Gal4 promoter, which consists of two domains, one is AD, the other is BD. When AD and BD are close enough to each other, the report gene transcription LacZ will be trigged. We selected and modified two peptides that have specific interactions with MCs and engineered them into two vectors to construct the Yeast Two-hybrid system. In the presence of MCs at different concentrations, blue dots in different shades of colors can be seen directly. <br />
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===[[Team:Todai-Tokyo | Team Todai-Tokyo:]] Prevention of Lifestyle Diseases Using Synthetic Organisms===<br />
Lifestyle diseases, diseases caused by unhealthy living habits, comprise one of the major problems in modern society, especially as they may lead to fatal heart problems or even cancer. However, preventing or curing these diseases is presently of extreme difficulty. Our team, Todai-Tokyo, has been tackling treatment of lifestyle diseases such as hypercholesterolemia, diabetes, circadian rhythm dysfunction, and bad smoking habits by using synthetic living systems, utilizing their ability to incorporate complex logic functions and dispensability of external control once in operation. To do this, we aim to create the following: cells that ingest cholesterol to decrease blood cholesterol levels, healthy low calorie breads, a system in which periodic gene expression is controlled, and bacteria that encourage smokers to quit smoking, respectively. By applying similar synthetic biology methodologies to these, prevention of numerous lifestyle-related diseases may become reality, serving as a first step towards their eradication.<br />
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===[[Team:Tokyo_Tech | Team Tokyo_Tech:]] 2009 Space Odyssey: Terraforming of Mars with genetically engineered bacteria===<br />
Have any life forms existed on Mars? If so, what kind of features could they have possessed? Today, the Martian environment is severe for any life to inhabit because of some constrained conditions. For instances, the surface temperature having a range from -80℃ to 15℃, CO2 occupying 95% of the atmosphere and the absence of organic substances on the surface don't allow aerobic organisms or heterotrophic bacteria to grow. Our project objective is to create a genetically engineered iron-oxidizing bacteria surviving on Mars and to establish a new model organism playing an important role to terraform Mars. We engineered Acidithiobacillus ferrooxidans by introducing a synthetic pathway of both Melanin and Anti Freeze Protein with temperature-regulated systems. Anti Freeze Protein contributes to enhance tolerance of cryogenic condition and Melanin to blacken the Martian surface eventually resulting in melt of ice cap and generation of atmosphere and sea. <br />
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===[[Team:Tokyo-Nokogen | Team Tokyo-Nokogen:]] Escape tedious work with Escherichia coli Auto Protein Synthesizer (ESCAPES).===<br />
Tokyo-NokoGen has developed the Escherichia coli Auto Protein Synthesizer (ESCAPES), an E. coli machine that greatly simplifies the production of your favorite protein. We created a green light-activated actuator to respond to external light signals, as well as a riboregulator-based signal counter to count the number of flashes. In ESCAPES, the first green light flash induces the E. coli to self-aggregate, while the second flash causes them to auto-lyse, thus greatly simplifying the protein preparation process. The light-activated actuator was constructed by fusing the light responsive domain of the Synechocystis photoreceptor CcaS with the EnvZ histidine kinase domain. Self-aggregation is achieved by the induction of the Antigen43 gene, which we isolated from E. coli, while autolysis took advantage of the available BioBrick parts endolysin and holin. ESCAPES helps you “escape” from tedious protein preparation steps, such as centrifugation and cell disruption. <br />
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===[[Team:TorontoMaRSDiscovery | Team TorontoMaRSDiscovery:]] Engineering bacterial micro-compartments to investigate metabolic channeling and its potential uses in biotechnological applications===<br />
A key challenge in metabolic engineering is to improve productivity and yield. Potential applications range from the production of valuable compounds such as therapeutic molecules and biofuels to the degradation of toxic wastes. There is increasing recognition that spatial organization can play an important role in optimizing pathway efficiency. Specifically, the spatial co-localization of consecutive enzymes in a pathway can result in efficient translocation of substrates between enzymes, an effect known as enzyme "channeling". Here we report the design, modeling and construction of a bacterial micro-organelle based system for the targeted co-localization of selected enzymes. Our "Encapsulator" represents a fundamentally new class of parts which, in nature consist of metabolic enzymes encased within a multi-protein shell reminiscent of a viral capsid. Micro-compartments based on encapsulin (and similar proteins) represent an experimentally amenable system to investigate the effects of channeling in potential downstream applications.<br />
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===[[Team:Tsinghua | Team Tsinghua:]] Syn-genome Based Gensniper===<br />
Our aim is to construct a targeted gene therapy vector with high cellular specificity, considerable capacity and the potential for mass production and universal modification. Analogizing the characteristics of bacteriophage lambda and adenovirus, we genomically engineered the fiber protein of adenovirus with the pC of bacteriophage lambda, with the knob region modified by cell-specific peptides generated by phage display (called targeted biobrick). After inducing the vector genome (generated by bottom-up or top-down approach) into BL21 DE3 E.coli strain, we applied a co-transformed therapeutic DNA (namely a cosmid with a capacity of 40-50 kb) for mass production of our targeted gene therapy vectors containing the desired genes to be delivered. With the targeted biobrick immediating the attachment and RGD domain immediating the internalization of the targeted vector, we are able to accomplish the targeted gene therapy.<br />
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===[[Team:TUDelft | Team TUDelft:]] Bacterial Relay Race===<br />
In our project, we aim at creating a cell-to-cell communication system that allows the propagation of a set of instructions coded on a plasmid, and not just binary information as in quorum sensing. To achieve this goal, we have designed a communication system based on three different modules: a conjugation system, a time-delay genetic circuit, and a self- destructive plasmid. Cell-to-cell communication systems are important because, in most synthetic biology applications, the desired tasks are generally accomplished by a population of cells, rather than by a single cell. The proposed communication system could be used for creating a distributed sensors network, or it could help to better understand and possibly reduce antibiotic resistance in bacteria. Furthermore, we have conducted a survey to study the perception on synthetic biology and related ethical issues, among iGEM participants, students and supervisors. We have focused on the top-down and bottom-up approaches as applied to biology.<br />
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===[[Team:TzuChiU_Formosa | Team TzuChiU_Formosa:]] Midnight Apollo===<br />
In Taiwan there are 9 power stations generating energy by coal, and produce 269.1 million tons of CO2 every year. Power stations are major causes of global warming. Therefore, we would like to create a ” biolight” system that can reduce CO2 production and attenuate degree of global warming. We plan to create a new organism that doesn’t need electricity and cause no pollution. We named it “Midnight Apollo”. The “Midnight Apollo” will be turned on when surrounding area turns dark and will be turned off automatically when the environment becomes bright. The idea is based on two systems, Cph8 and aeoquorin/GFP. The Cph8 is regulated by visible light that can activate protein translation of an illuminating system. Subsequently, this illuminating system would use aeoquorin/GFP to light up the environment. We hope The Midnight Apollo could be applied in producing energy-saved Bio-streetlamp, emergency Biolighting, or Biosearchlight.<br />
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===[[Team:UAB-Barcelona | Team UAB-Barcelona:]] A toxics biosensor. Could bacteria detect instantaneous and simultaneously several types of pollutants?===<br />
We would like to construct a recombinant /Escherichia coli /strain that/ /could detect different aggressive pollutants, like toxics compounds. The first approach was the halogenated compounds (and more specifically chloroform) detector. Tap water usually contains it due to the chlorination process in drinking water production or other activities like swimming pools, etc. and it can become harmful to public health at high concentrations. /Nitrosomonas/ /europaea/’s promoters (/mbla/ and /clpb/) are specifically sensitive to chloroform, so coupling them with an output (a fluorescence protein) should allow quantifying its concentration. This was our first approximation towards our final aim of making a complete circuit that would allow to assign simultaneously to every pollutants family a certain color (fluorophore) thanks to the selective activation of different promoters.<br />
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===[[Team:UC_Davis | Team UC_Davis:]] A Bacterial Secretion System Motivated the Goal of Managing Celiac===<br />
The current estimate of the number of Americans with Celiac Disease/gluten intolerance is one out of 133. Not being able to digest gluten properly inside the small intestine leads to an immune system response that leads to a variety of symptoms. We have designed a bacterial secretion system that could be used in a probiotic organism to secrete an enzyme to degrade the allergen gliadin before it reaches the intestine. A putative advantage a probiotic treatment over direct enzyme therapy approaches is the potential for administering fewer doses, thus making it less troublesome, less costly, and more convenient. Our secretion system consists of an inducible promoter, ribosome binding site, an extracellular anchor (ompA/INPNC), cleavage signal sequence, 6 HIS Tag and a terminator. We have tested its behavior in E. coli on two proteins of varying sizes (GFP/Luciferase). <br />
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===[[Team:UChicago | Team UChicago:]] An enhanced yeast-based system for detection and decontamination of organophosphate neurotoxins.===<br />
Organophophates (OPs) are highly toxic compounds used as pesticides and chemical ware-fare agents around the world, including sarin, soman, and VX gas. To combat these toxins, which act as acetylcholinesterase inhibitors, we designed a highly efficient whole-cell S. cerevisiae sensor and biocatalyst system for the detection and remediation of the model organophosphate compound paraoxon and its degradation products. For our biosensor device, reporter constructs were incorporated into the genome downstream of paraoxon and paraoxon-hydrolyis sensitive promoters. Our degradation device was designed in three parts, targeting paraoxon through expression of organophosphate hydrolase from Flavobacterium, sp and two of its degradation byproducts p-nitrophenol and diethyl phosphate. Each device was genomically integrated in order to bypass selective the need for selective condition, while the degradation devices were constitutively expressed for maximum efficiency. Combined, this two-part system allows for both the detection and remediation of a broad range of common and deadly neurotoxins.<br />
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===[[Team:UCL_London | Team UCL_London:]] Stress Light===<br />
Our project “Stress Light” will produce a series of synthetic biosensor devises, which can improve on the traditional sensors in bio-processing; by using green fluorescent protein (GFP), yellow fluorescent protein (YFP), cyan fluorescent protein (CFP) and red fluorescent protein (RFP) expression as indicators of different stresses for e.coli bacteria during cultivation. Our product and system is called “the traffic-light stress sensor”. It is constructed to express fluorescent proteins of different colors in response to different stimuli which are inhibiting growth or harming production. We aim to build a sensor that can detect shear stress and low oxygen levels. We believe these two stresses are critical in a bioprocessing environment. We are applying e.coli’s native or modified promoters for DegP, Spy and NarK in order to induce transcription. Ideally the cells responses to stressed conditions should be sufficiently accurate, reliable and rapid for the stresses to be detected and mitigated.<br />
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===[[Team:UCSF | Team UCSF:]] Engineering the Movement of Cellular Robots===<br />
Some eukaryotic cells, such as white blood cells, have the amazing ability to sense specific external chemical signals, and move toward those signals. This behavior, known as chemotaxis, is a fundamental biological process crucial to such diverse functions as development, wound healing and immune response. Our project focuses on using a synthetic biology approach to manipulate signaling pathways that mediate chemotaxis. We are attempting to reprogram the movements that the cells undergo by altering the guidance and movement machinery of these cells in a modular way. For example, can we steer them to migrate toward new signals? Can we make cells move faster? Slower? We hope to better understand how chemotaxis works, and eventually build cells that can perform useful tasks. Imagine, for example, therapeutic cell-bots that could home to a directed site in the body and execute complex, user-defined functions (e.g., kill tumors, deliver drugs, guide stem cell migration). <br />
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===[[Team:ULB-Brussels | Team ULB-Brussels:]] GluColi, a new generation of glue===<br />
Whether you want to stop a leaking ship’s hull, or repair a fractured bone, you will need a top quality, strong adhesive. Our project aims to create a new generation of glue. In contrast to most glues, ours is natural, biodegradable, efficient on wet surfaces and has exceptional resistance (up to 3 times better than super glue). It is composed of a polysaccharide released by a Gram-negative bacterium, Caulobacter crescentus. Our aim is to use BioBrickTM standard biological parts in order to create a new strain of Escherichia coli which will synthesise this adhesive material. Moreover we are going to use a new plasmid stabilisation technique, the StabyTM system developed by Delphi Genetics. This system allows us to avoid the use of antibiotics and has been shown to be more efficient as far as protein secretion is concerned.<br />
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===[[Team:UNICAMP-Brazil | Team UNICAMP-Brazil:]] The Microguards===<br />
Industrial production processes based on microorganisms, such as biofuels, fine chemicals and enzymes, are threatened by contaminants that cause losses of up to 10% of the gross production. To solve this problem, the aim of our project is to engineer strains of the industrial workhorses E. coli and S. cerevisiae to recognize and destroy contaminants. The engineered yeast will recognize lactic acid bacteria contaminants, which greatly affect Brazilian´s ethanol production. The presence of lactic acid will activate a lysozyme-based killing mechanism, effective against gram-positive contamination. The engineered E. coli will recognize contaminants based on a non-self recognition mechanism and a percentage of the population will differentiate into a killing lineage releasing colicins and endonucleases. The killing mechanisms will be regulated by promoter Py, probably activated by conjugation. The characterization of Py and a lactate-inducible promoter that is not subjected to glucose catabolic repression are the main challenges of our project.<br />
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===[[Team:UNIPV-Pavia | Team UNIPV-Pavia:]] Ethanol? Whey not!===<br />
Cheese whey is classified as a special waste for its high biochemical and chemical oxygen demand. Even if whey can be valorized by extracting high value substances, like whey-proteins, at the end of the treatment the residual liquid is still a special waste for its high lactose content (4%). E. coli was engineered to convert efficiently lactose into ethanol, a precious biofuel. Three main enzymes are involved in this transformation: beta-galactosidase, pyruvate-decarboxylase and alcohol-dehydrogenaseII. Beta-galactosidase (lacZ gene) was over-expressed to obtain higher lactose-glucose conversion yield. Coding sequences of pyruvate-decarboxylase (pdc) and alcohol-dehydrogenaseII (adhB), essential in alcoholic fermentation pathway, were designed by DNA chemical synthesis and codon-optimized for E. coli. The final circuit includes the device to metabolize lactose and the ethanol-producing operon, containing pdc and adhB. It has a theoretical yield of 20kilos/tons of whey. Finally, 3OC6HSL, aTc and lactose/IPTG inducible systems were characterized to be used in this circuit.<br />
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===[[Team:uOttawa | Team uOttawa:]] A probiotic Lactobacillus strain which produces cellulose===<br />
This year’s project focuses on genetically engineering the bacterium Lactobacillus plantarum to produce cellulose, as a food additive. L. plantarum was selected as it is already commonly found in yogurt. The aim of generating this novel probiotic is to reduce human morbidity via the subsequent increase in dietary fibre in the gut. The sequestering of glucose for fibre production by L. plantarum provides the additional benefit of effectively reducing dietary sugars. We have successfully extracted the four genes that code for cellulose synthase from the Acetobacter xylinum. These genes were then placed under the control of a strong constitutive promoter, and transformed into Lactobacillus plantarum. Plasmid and genome based expression of the synthase genes are being evaluated and characterized. In the future, cellulose production assays, evaluation of biofilm formation, and in vivo testing will be performed to determine viability as potential health benefits.<br />
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===[[Team:Uppsala-Sweden | Team Uppsala-Sweden:]] Booze Bugs : Sun To Alcohol===<br />
In the long run our crude oil resources will be on the decline but most importantly the effects of the climate change demand a quick shift to a sustainable fuel economy. Approaching biofuel production by direct synthesis from sunlight has the potential to solve the problems that arise with the conventional fermentation of starches and sugars such as the direct competition of fuel feedstock with food crops. Thus the Uppsala iGEM Team 2009 investigated the production of ethanol and butanol with the use of the cyanobacteria Synechocystis sp PCC6803. Also known as blue-green algae, cyanobacteria possess the ability to directly convert sunlight into biofuels. We engineered constructs for ethanol and butanol production as well as strategies to increase the yields of photosynthetic ethanol production.<br />
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===[[Team:UQ-Australia | Team UQ-Australia:]] Mercury sequestration using a multicomponent operon, and increasing the temperature tolerance range of P. syringae.===<br />
Microbes such as Escherichia coli and Cuprivadis metallidurans have an endogenous multicomponent mercury (Hg2+) uptake and reduction operon, under the control of a metal responsive transcription factor, MerR. By utilising elements of this pathway, with a novel recovery mechanism, mercury can be accumulated intracellularly and efficiently removed from the environment. The presence of mercury activates MerR, driving the expression of Antigen 43 (Ag43), a self-adhering surface protein. Coupling a mercury sensitive promoter to the expression of Ag43 enables cells to accumulate mercury then aggregate in solution. P. syringae is a ubiquitous airborne bacterium which expresses a unique protein, InaZ. This protein acts as a scaffold for ice nucleation, inducing precipitation. Optimal growth of P. syringae occurs at 22oC. By introducing five heat-shock genes, the tolerance range will be increased to better suit the Australian climate. This modification has the potential to increase the availability of Australia’s most precious resource; water.<br />
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===[[Team:USTC | Team USTC:]] E. coli Automatic Directed Evolution Machine: a Universal Framework for Evolutionary Approaches in Synthetic Biology===<br />
Evolution is powerful enough to create everything, from biomolecules to ecosystems. The ultimate goal of E. coli Automatic Directed Evolution Machine (E.ADEM) project is to manage the power of evolution, by engineering a robust system framework that can automatically create anything we want in synthetic biology, from various types of parts to complex systems. Each demand can be converted into designing a scoring function to give the evolution process a direction. E.ADEM is designed by implementing evolutionary algorithm back into biology. The core of E.ADEM is a self-adaptive controller that can adjust variation rate and selection pressure, based on fitness score, population size and average fitness score calculated by a quorum sensing device. After comprehensive measurement using constitutive promoter family stimulus signals and modeling of the components, a prototype machine is built. Modular design and PoPS device boundary standard will ensure the extensibility and universality of the machine.<br />
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===[[Team:USTC_Software | Team USTC_Software:]] Automatic Biological Circuit Design===<br />
The ultimate goal of synthetic biology is to program complex biological networks that could achieve desired phenotype and produce significant metabolites in purpose of real world application, by fabricating standard components from an engineering-driven perspective. This project explores the application of theoretical approaches to automatically design synthetic complex biological networks with desired functions defined as dynamical behavior and input-output property. We propose a novel design scheme highlighted in the notion of trade-off that synthetic networks could be obtained by a compromise between performance and robustness. Moreover, series of eligible strategies, which consist of various topologies and possible standard components such as BioBricks, provide multiple choices to facilitate the wet experiment procedure. Description of all feasible solutions takes advantage of SBML and SBGN standard to guarantee extensibility and compatibility.<br />
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===[[Team:Utah_State | Team Utah_State:]] BioBricks without Borders: Investigating a multi-host BioBrick vector and secretion of cellular products===<br />
The aim of the Utah State University iGEM project is to develop improved upstream and downstream processing strategies for manufacturing cellular products using the standardized BioBrick system. First, we altered the broad-host range vector pRL1383a to comply with BioBrick standards and enable use of BioBrick constructs in organisms like Pseudomonas putida, Rhodobacter sphaeroides, and Synechocystis PCC6803. This vector will facilitate exploitation of advantageous characteristics of these organisms, such as photosynthetic carbon assimilation. Following expression, product recovery poses a difficult and expensive challenge. Downstream processing of cellular compounds, like polyhydroxyalkanoates (PHAs), commonly represents more than half of the total production expense. To counter this problem, secretion-promoting BioBrick devices were constructed through genetic fusion of signal peptides with protein-coding regions. To demonstrate this, the secretion of PHA granule-associated proteins and their affinity to PHA was investigated. Project success will facilitate expression and recovery of BioBrick-coded products in multiple organisms.<br />
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===[[Team:Valencia | Team Valencia:]] iLCD: iGEM Lighting Cell Display===<br />
The Valencia Team project consists of developing a “bio-screen” of voltage-activated cells, where every “cellular pixel” produces light. It is known that for instance neurons, cardiomyocites or muscle cells are able to sense and respond to electrical signals. These cells use a common second messenger system, calcium ion, which promotes a defined response when an electrical pulse is supplied to them. Nevertheless, these cultures present several technical disadvantages in order to make a handily use of them. Valencia team uses this property on yeast to produce luminescence as a response to electrical stimulus. This project constitutes the first time in which the electrical response of Saccharomyces and its potential applications are going to be tested. The obtained results will be used to build the first iLCD in history. We will reflect the perception that different groups of people have about Synthetic Biology in the survey http://igemvalencia.questionpro.com. <br />
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===[[Team:Victoria_Australia | Team Victoria_Australia:]] An environmentally sustainable biological lighting system ===<br />
Our aim is to build a biological lighting system via cell free transcription and translation. We will be focusing on developing a prototype using two cell free systems: E. coli and wheat germ in which the proteins will fluoresce. We are using the fluorescent proteins BFP, GFP, Vic green, blueberry, yellow and cherry in the cell free systems. Our main aim was to develop an alternative light source, which could possibly be powered by a waste material as simple as grass clippings (cell lysate). We are also attempting to develop a new registry part that is a blueberry fluorescent protein using the yellow protein (part # BBa_E0030) through mutagenesis. <br />
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===[[Team:VictoriaBC | Team VictoriaBC:]] Signal Integration: Applications of RNA Riboregulator Capabilities===<br />
This project explores some of the ways that the secondary structure of messenger RNA can be used to control the rate of protein expression. The 32<sup>o</sup>C ribothermometer made by the 2008 TUDelft team will be coupled to fluorescent proteins to visually confirm temperature-dependent translation. The "ribolock" made by the 2006 Berkeley team will be tested at various temperatures to determine if it could double for use as a ribothermometer. Finally, a proof-of-concept NAND logic gate will be constructed: a ribolock will be used to interpret two concurrent environmental signals into an on/off control for mCherry output.<br />
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===[[Team:Virginia | Team Virginia:]] Arsenic Sequestration for Groundwater Decontamination===<br />
As many as 137 million people in 70 countries may be affected by groundwater contaminated with arsenic. Existing treatment options are too expensive for the majority of affected areas. Therefore we are developing a bioremediation tool using Escherichia coli to absorb and bind arsenic and remove it from its surrounding environment. Natural and synthetic peptides are employed to sequester the toxic ions and a pump knockout ensures that arsenic stays in the cell. Measurement of growth capacity of the engineered strain in arsenic containing media and quantitative analysis of arsenic sequestration will be performed. Characterization and integration of an arsenic-responsive promoter will allow the sequestration system to dynamically adjust to current conditions. A simple, well-implemented system for biosequestration of arsenic may become part of a solution to a problem denying access to clean drinking water for many.<br />
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===[[Team:Virginia_Commonwealth | Team Virginia_Commonwealth:]] Promoter design, characterization and consequences===<br />
The generation of well-characterized genetic parts is a prerequisite for the rational design and construction of reliable genetically-encoded devices and systems. However, most publicly available parts (including those in the Registry) remain largely uncharacterized. Therefore, we propose a minimal measurement standard for the quantitative characterization of one of the most frequently used parts, promoters. This approach uses both mRNA and protein measurements to provide a tractable and universal analysis of relevant promoter characteristics. In an effort to elucidate promoter design principles, we have also designed and characterized new promoter and enhancer sequences. Our goal is to contribute to the advancement of fundamental synthetic biology by evaluating the performance of new and existing promoters and enhancers, which may serve as a model for describing other basic parts such as ribosome binding sites and transcriptional terminators.<br />
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===[[Team:Warsaw | Team Warsaw:]] BacInVader – a new system for cancer genetic therapy===<br />
The main aim of our project is to design a model system based on genetically modified Escherichia coli, capable of entering into eukaryotic cells. We have developed a regulatory system composed of three distinct functional modules. The whole system is activated by thermal degradation of the repressor protein, which leads to internalisation of E. coli by mammalian cells. When in endosome, pH-dependent two-component regulatory system activity enables the bacterium to escape to cytoplasm. Once the bacterium is in the cytoplasm some proteins are secreted due to expression of specific genes. In our case, secretion of p53 or bax proteins to mitochondria leads to apoptosis without cell cycle arrest thus enabling complementation of traditional chemotherapeutical agents, which affect only proliferating cells.<br />
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===[[Team:Wash_U | Team Wash_U:]] Improved Photosynthetic Productivity for Rhodobacter sphaeroides via Synthetic Regulation of the Light Harvesting Antenna LH2===<br />
Photosynthetic light harvesting antennas function to collect light and transfer energy to a reaction center for photochemistry. Phototrophs evolved large antennas to compete for photons in natural environments where light is scarce. Consequently, cells at the surface of photobioreactors over-absorb light, leading to attenuated photobioreactor light penetration and starving cells on the interior of photons. This reduction of photosynthetic productivity has been identified as the primary impediment to improving photobioreactor efficiency. While reduction of antenna size improves photosynthetic productivity, current approaches to this end uniformly truncate antennas and are difficult to manipulate from the perspective of bioengineering. We aim to create a modifiable system to optimize antenna size throughout the bioreactor by utilizing a synthetic regulatory mechanism that correlates expression of the pucB/A LH2 antenna genes with incident light intensity. This new application of synthetic biology serves to transform the science of antenna reduction into the engineering of antenna optimization. <br />
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===[[Team:Washington | Team Washington:]] The Ideal Protein Purification System===<br />
The use of recombinant protein production using E. coli-based expression systems has revolutionized the fields of biotechnology and medicine. However, the ability to utilize such proteins hinges upon their capacity to be isolated from their expression systems. Our project aims to create an all-in-one protein expression and purification system using BioBrick standards to greatly simplify protein production for synthetic biologists, reducing the time and cost involved in standard protein purification methods. Our method uses a novel combination of two systems: secretion and display. By fusing two tags to the protein it can be secreted into the expression media, and subsequently directed to bind to the outside of the cell. To collect the pure proteins, cells only need to be spun down and then resuspended in an elution buffer, releasing the protein of interest. Our research exhibits the utility of synthetic biology for developing new techniques that improve upon established practices.<br />
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===[[Team:Washington-Software | Team Washington-Software:]] LegoRoboBricks for Automated BioBrick Assembly===<br />
Commercial Liquid Handling Systems are extremely expensive, and are typically beyond the reach of the average molecular biologist interested in performing high throughput methods. To address this problem, we design and implement a liquid handling system built from commonly accessible Legos. Our goal is the automation of BioBrick assembly on a platform that can itself be easily replicated and we demonstrate a proof-of-principle for this system by transferring colored dye solutions on a 96-well plate. We introduce a new concept called LegoRoboBrick. The liquid handling system is build from 3 new LegoRoboBrick modular components: ALPHA (Automated Lego Pipette Head Assembly), BETA (BioBrick Environmental Testing Apparatus), and PHI (Pneumatic Handling Interface). We will demonstrate that the same BioBrick assembly software can run on multiple plug-and-play LegoRoboBrick instances with different physical dimensions and geometric configurations. The modular design of LegoRoboBricks allows easy extension of new laboratory functionalities in the future.<br />
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===[[Team:Waterloo | Team Waterloo:]] Chromobricks: A Platform for Chromosome Engineering with BioBricks===<br />
The aim of our project is to develop a fully-featured platform for chromosome engineering, allowing the in vivo assembly of a synthetic chromosome from interchangeable parts, followed by selective degradation of the native chromosome. We have designed a proof-of-concept for chromosome-building that will use the site-specific integrase of phage ΦC31 to integrate a BioBrick into a defined locus of the E. coli genome. Six pairs of integrase-targeted att sites have been designed to be non-cross-reactive in order to support repeatable cassette-exchange reactions for chromosome building. We have also written software to model the integrase-mediated rearrangement of DNA molecules containing att sites, to aid the design of more elaborate chromosome-building systems. To selectively degrade the native chromosome we designed a nuclease-based, inducible genome-degradation system. In its simplest form, our system can be used to integrate biological devices into a chromosome in situations requiring stable copy number and selection-free maintenance.<br />
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===[[Team:Wisconsin-Madison | Team Wisconsin-Madison:]] Ocean Fuel: increased salt tolerance through glycine betaine production===<br />
Biofuels represent a potential solution to current world energy demands. Total crude oil replacement based on a 20% fuel titer and current fuel demands would require 5.6 trillion gallons of fresh water per year. Current fresh water supplies may not support this added demand. Alternatively, a sustainable approach may use a portion of the Earth’s 3.5x1020 gallons of ocean water. However, current fuel-producing organisms are unable to thrive in ocean-level osmolarities. Glycine betaine, a powerful osmoprotectant, shields organisms from salt-induced stress. Wild-type Escherichia coli can acquire glycine betaine from their surroundings or synthesize it from environmental choline. Two enzymes, glycine/sarcosine methyltransferase, and sarcosine/dimethyglycine methyltransferase, catalyze three successive methylations of glycine for de novo synthesis of glycine betaine. Here, we demonstrate an engineered E. coli with an increased growth rate under salt induced stress. We highlight utility by demonstrating the improved growth of fuel producing bacteria in ocean water.<br />
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===[[Team:Yeshiva_NYC | Team Yeshiva_NYC:]] Spatially encoding temporal information: using diffusional escape of periplasmic reporter proteins as a clock.===<br />
We were inspired by iGEM projects that utilize colored or fluorescent reporter molecules. Specifically, we thought “wouldn’t it be nice to be able to leave a plate out in the field, collect it a day or two later, and be able to tell at what time the synthesis of the reporter molecules was triggered?” Ideally, protein reporters would leave the cell and diffuse through the agar, leaving a clear history of their expression. To this end, we are 1) creating a library of Sec and Tat leader sequence biobricks for directing expressed proteins to the periplasm, 2) making an E. coli expression strain with a weakened outer cell wall that leaks most of the periplasmic proteins to the environment, and 3) measuring and modeling the diffusion of small molecules and proteins in agar to ascertain the ability to derive the times and conditions under which they originated.</div>Meaganhttp://2009.igem.org/Jamboree/Project_Abstract/Team_AbstractsJamboree/Project Abstract/Team Abstracts2009-11-05T15:11:32Z<p>Meagan: </p>
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===[[Team:Aberdeen_Scotland | Team Aberdeen_Scotland:]] A Synthetic Biology Approach to Pipe Repair: The Pico-Plumber===<br />
Damage to inaccessible pipe systems, such as computer cooling circuits, is difficult to rectify. An Escherichia coli synthetic biology circuit for pipe repair was designed. Pipe breach detection and the restoration of pipe integrity were implemented through exploitation of chemotaxis, and cell lysis that releases a two-component protein-based glue (lysyl oxidase and tropoelastin). Control was achieved using an AND gate with quorum sensing and the lac inducer IPTG (released from the breach) as inputs. Deterministic and stochastic models of the genetic circuit, integrated with an agent-based model of E.coli cells, were used to define the effective radii of cell migration and timing of lysis. Constructed AND gate, quorum sensing and lysis timing modules were experimentally tested. The two-component glue concept was successfully validated using in vitro alpha-omega complementation of beta-galatosidase activity. Finally, a proposal for an igem.org-based parameter database was developed to aid the rapid identifation of BioBricks parameter values.<br />
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===[[Team:Alberta | Team Alberta:]] A Synthetic Biology Tool Kit for Artificial Genome Design and Construction===<br />
The creation of simplified artificial cells with specialized functions, along design principles that are compatible with the goals of synthetic biology, requires advances in two key areas. In Silico modelling tools are needed to assess the performance of artificial networks prior to assembly. Genome biofabrication must achieve rates well beyond existing methods using a modular design so that the extent to which natural systems can be made artificial can be tested. We have taken our first steps towards these goals by directing our efforts to the rational refactoring of the E. coli genome. Using flux balance analysis we have identified 117 new genes that may be essential for survival. We have developed and validated a rapid, modular biofabrication method (BioBytes) and have produced BioBytes for 150 of our 447 essential gene list. We have also built a Lego Mindstorm-based DIY biofab robot and extended the concept to a BioFab-on-a-chip prototype.<br />
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===[[Team:ArtScienceBangalore | Team ArtScienceBangalore:]]===<br />
We consider ourselves amateurs/novices within the context of the IGEM competition. Our endeavor as “outsiders” is to bring our training in the arts and design to synthetic biology. Over this summer, we learnt the tools and techniques of synthetic biology and developed a piece of life which reflects our concerns, namely, the cultural, ethical and aesthetic implications of Synthetic Biology. Using a DIY approach and getting our hands “wet” was a critical element in the learning process. Our construct synthesizes Geosmin, an enzyme normally produced by cyanobacteria and actinobacteria. The biosynthesis of geosmin from farnesyl diphosphate is catalyzed by a single enzyme germacradienol/germacrene D synthase.E. coli, does not bear a gene that codes for this enzyme. We have expressed this gene in different strains of E. coli. Geosmin is responsible for producing the earthy smell when rain falls after a dry spell of weather.<br />
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===[[Team:Bay_Area_RSI | Team Bay_Area_RSI:]] Breast cancer cell targeting phage===<br />
Breast cancer is the second most common type of cancer diagnosed in women. RNAi has proven to be an effective mechanism in the silencing of oncogenes. Therefore, we have attempted to build a viable system for the delivery of RNAi into breast cancer cells. First, we inserted a shRNA sequence coding for the Raf-1 protein into an AAV cassette containing two ITR's, allowing it to reproduce itself in mammalian cells. This cassette was inserted into our chosen vector, the filamentous bacteriophage FUSE-55. An antibody sequence was then added to the phage plasmid near the coat protein sequence in order to target HER2. As an additional feature, we have fused Silicatein and Silintaphin to mStrepavidin, which will bind to a protein tag in the coat, forming silicate structures on the coat of the phage, thereby reducing the immunotoxicity of the bacteriophage in vivo.<br />
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===[[Team:BCCS-Bristol | Team BCCS-Bristol:]] VESECURE=== <br />
Directed delivery of specific proteins into cells would have dramatic consequences for drug delivery and expand the horizons of synthetic biology into the multicellular domain via discrete, targetted communication. Gram-negative bacteria naturally produce outer member vesicles (OMVs): spherical, bilayered proteolipids from 20-200nm in diameter. OMVs carry outer membrane, periplasmic and cytoplasmic proteins, DNA, RNA and other biological molecules. They protect their cargo from the extracellular environment and deliver it to a multitude of target cells via membrane fusion. We investigate the possibility of allowing the secretion of any protein in OMVs via fusion with novel, non-toxic partners enhanced in OMVs, using a novel Bioscaffold compatible with the current assembly standard. A new version of the award winning BSim software has been developed to study applications at the population level such as communication. The ultimate goal is to create a safe and standardised system for directed delivery of proteins into cells.<br />
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===[[Team:Berkeley_Software | Team Berkeley_Software:]] Eugene, Spectacles, and Kepler: Managing Synthetic Biology Device Development===<br />
Three crucial activities in synthetic biology are the creation of standardized parts, the construction and specification of devices from these parts, and the automatic assembly of these devices. Each of these activities requires software tools. Tools give users access to data as well as provide algorithmic support and abstraction to design large scale systems. We have created three software tools for these tasks. The first is a domain specific language called Eugene for the specification of biological constructs and rules for their creation. The second contribution is a visual design environment for device creation called Spectacles. Finally, we have created workflows for the Kepler design environment. This work is integrated within the Clotho design framework. We show that together they offer a powerful solution to the problems of today while also providing a path to the more exotic design activities of the future.<br />
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===[[Team:Berkeley_Wetlab | Team Berkeley_Wetlab:]] Automated assembly of cell surface display devices===<br />
The University of California Berkeley iGEM team has developed an automated approach to large-scale parts assembly that is accurate, high-throughput, reduces labor, and decreases cost. As a test bed for our system we have chosen to explore novel applications of cell surface display within Escherichia coli, the gold standard organism for bacterial engineering. Displaying peptides and proteins on a cell's surface is difficult, and many attempts may have to be made to generate a given functional protein. By automatically generating and testing a large set of diverse proteins paired with various display methods, we can search a large design space and develop guidelines for rational design of projects involving surface display. <br />
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===[[Team:BIOTEC_Dresden | Team BIOTEC_Dresden:]] Temporal and spatial control of protein synthesis by in vitro recombination inside picoliter reactors===<br />
Manufacturing functionalized proteins in vitro poses a challenge, as it requires coordinated molecular assemblies and multi-step reactions. In this project we aim to control, over time and space, the production of proteins tagged with a silver-binding peptide for in situ silver nanoparticle nucleation inside microdroplets generated by microfluidic devices. Combining a transcription-translation system with protein coding genes and a recombination logic inside microdroplets provides spatial control. Moreover, in the microfluidic chamber we can pinpoint the beginning of synthesis, and easily track and isolate the droplets. Site-specific recombination generates a molecular timer for temporal control of protein synthesis. Unlike transcriptional regulation, this method gives true all-or-none induction due to covalent modification of DNA by Flp recombinase. Determining the transfer curve of inter-FRT site distance versus average recombination time allows the onset of gene expression to be predicted. We then apply this Flp reporter system as a powerful PoPS measurement device.<br />
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===[[Team:Bologna | Team Bologna:]] T-REX: Trans-Repression of Expression. A BioBrick gene-independent control of translation===<br />
The project aims to realize a device with standard biological parts for the post-transcriptional control of gene expression, regardless of the gene sequence to be silenced. We designed the T-REX device, composed of two non-coding DNA sequences: the TRANS-repressor and the CIS-repressing parts. TRANS-repressor acts as a silencer of CIS-repressing RNA target. This target includes a region complementary to the TRANS-repressor sequence antisense, ends with RBS, and is assembled upstream of the coding sequence to be silenced. Upon binding of TRANS-repressor and CIS-repressing RNAs, the access to RBS by ribosomes is hampered, silencing translation. Accordingly, the amount of TRANS-repressor controls the translation rate of the regulated gene. The TRANS-repressor sequence was determined by a computational analysis performed to minimize the interference with the genomic mRNAs and to maximize the base-pairing interaction to the CIS-repressing RNA. The T-REX device is proposed as a universal and fast switch in synthetic gene circuits.<br />
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===[[Team:British_Columbia | Team British_Columbia:]] Development of a modular, analog E. coli biosensor===<br />
To date, efforts to design a whole-cell biosensor capable of detecting levels of one or more biological inputs and responding in an analog mode has been elusive. We have designed a system of synthetic constructs implemented in an E. coli chassis that will allow detection of continuously varying levels of a single metabolic input and report on the concentration with qualitative output depending on threshold levels of the input. Our system design utilizes RNA-level hairpin hybridization and antisense technologies linked to various reporters. Because our approach is modular and does not depend on either endogenous protein processing or exogenous RNA, we envision that such a system could find applications in many different fields, including environmental sensing, detection of diagnostic of therapeutic biomarkers, and systems biology. <br />
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===[[Team:Brown | Team Brown:]] Engineering Staphylococcus Epidermidis to Secrete Recombinant Histamine Binding Protein in Response to Changing Histamine Concentration===<br />
The 2009 Brown iGEM Team aims to treat allergic rhinitis (hay fever) by engineering Staphylococcus epidermidis to secrete a histamine-binding protein, rEV131, in response to elevated histamine concentrations during an allergic attack. rEV131 was cloned from a species of tick, Rhipicephalus appendiculatus. We are putting the rEV131 gene into an endogenous element of human nasal flora, Staphylococcus epidermidis. rEV131 will have a secretion tag specific for S. epidermidis. To synchronize rEV131 production with elevation of histamine concentration, we are computationally designing a novel histamine receptor. This histamine-responsive receptor will induce expression of rEV131. Although S. epidermidis is a non-pathogenic species, when it reaches a certain population threshold it produces potentially hazardous biofilms. To mitigate this concern, we have engineered safety measures that prevent excessive growth by repurposing S. epidmidis’ natural population sensor to cue each cell’s "suicide" when a population has reached a dangerous size.<br />
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===[[Team:Calgary | Team Calgary:]] Reprogramming a Language and a Community===<br />
iGEM Calgary contributed a second quorum sensing (QS) system to the Registry. The Vibrio harveyi AI-2 QS signalling system has been engineered in Escherichia coli . Coupled with quorum quenching, our system allows us to target biofilm maintenance. The robustness of AI-2 signalling in E. coli was characterized in the lab and compared to data from mathematical models of the system built using the Matlab Simbiology toolbox and the emerging Membrane Computing framework in Mathematica. We also undertook community outreach projects in order to enhance the synthetic biology community. Specifically, the Second Life platform was used to create an educational tool to train future synthetic biologists in an accessible, user-friendly, virtual environment. Moreover, we examined the implications of our project in light of the recently proposed proactionary and precautionary frameworks with special focus on ethical, environmental, economic, legal and social (E3LS) impact.<br />
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===[[Team:Cambridge | Team Cambridge:]] E. Chromi: Triggering Pigment Production in E. Coli===<br />
Previous iGEM teams have focused on genetically engineering bacterial biosensors by enabling bacteria to respond to novel inputs, especially biologically significant compounds. There is an unmistakable need to also develop devices that can 1) manipulate the input by changing the behaviour of the response of the input-sensitive promoter, and that can 2) report a response using clear, user-friendly outputs. The most popular output is the expression of a fluorescent protein, detectable using fluorescence microscopy. But, what if we could simply see the output with our own eyes? The Cambridge 2009 iGEM team is engineering E. coli to produce different pigments in response to different concentrations of an inducer.<br />
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===[[Team:CBNU-Korea | Team CBNU-Korea:]] Essarker: An Essential Remarker for a Minimal, Synthetic Genome===<br />
It is challengeable to create a synthetic genome for fulfilling the needs of energy and food. Without the assistance of computing tools, moreover, it would be much more difficult to make the synthetic genome. We here propose a key tool to help the creation of a genome as the essential step. The goal of Essarker is to help users design a minimal genome synthesized through the fundamental frame comprising the essential genes of replication. Essarker is a standalone software to manage and retrieve required sequences of genomes, and explore the essential gene order and direction and the related orthologous genes. It also identifies and visualizes the positions and orientations of genes. In addition, it shows optimal ordering of essential genes and orthologs by statistical analysis.<br />
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===[[Team:Chiba | Team Chiba:]] E. coli Time Manager Since 2008===<br />
Since 2008, we have been constructing the bacteria timer that "work together". The mechanism is very simple; (1) the "Transmitter cells" generates the signal molecules, whose concentration gradually increases, (2) when it reaches a certain level, the "Receiver cells" switch on the expression of any given genes. Precise control of the time of delay of this entire process, one can pre-set the time of expression of genetic functions in a predicable manner. By using Asyl-Homoserine Lactones(AHLs) that can freely pass through the cell membrane as signal molecules, the time can be shared, in real time, by all cells within the pot. This way, receiver (timer) cells would take the action all at once in right timing, minimizing the distribution in each cell's response time. This year, we are trying to make a platform for generating an animated pictures using series of new timer cells we have constructed.<br />
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===[[Team:CityColSanFrancisco | Team CityColSanFrancisco:]]===<br />
We at CCSF have begun constructing a bacterial powered battery. The design has been generated with sustainability in mind, and aims to create an alternative to traditional fossil fuel technologies. The battery owes its capabilities to two strains of bacteria: the heterotroph Rhodoferax ferrireducens, and the photoautotroph Rhodopseudomonas palustris. Each strain will occupy its own concentration cell and after being cultured anaerobically, will either oxidize (in the case of R. palustris) ferris iron or reduce (in the case of R. ferrireducens) ferric iron. The resulting current will be collected and used to demonstrate the functionality of the battery. The reduction and oxidation reaction will be self-substaining. This process is further aided by the genetic modification of R. palustris. As a photosynthetic prokaryote, R. palustris generates glucose readily. We intend to share this glucose with R. ferrireducens by inserting a passive glucose transporter into the cells of R. palustris.<br />
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===[[Team:Cornell | Team Cornell:]] Engineering the Bacillus Subtilis Metal Ion Homeostasis System to Serve as a Cadmium Responsive Biosensor===<br />
The goal of our project is to create a whole cell cadmium biosensor by attaching cadmium responsive promoters in Bacillus subtilis to fluorescent reporter proteins. Cadmium is a toxic heavy metal which has no known biological function. Ingestion of cadmium contaminated water can induce bone fractures and severe renal damage. Major sources of cadmium contamination include fertilizers, sewage sludge, manure and atmospheric deposition. Cadmium contaminated sewage is often used for irrigation purposes in many parts of the world, especially in developing nations. Crops grown in these contaminated soils are then sold in markets without any detoxification treatment. Current analytical methods such as atomic absorption spectroscopy, though highly sensitive, are significantly more expensive than bacterial biosensors and are unable to measure the amount of bioavailable cadmium.<br />
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===[[Team:DTU_Denmark | Team DTU_Denmark:]] The redoxilator, and the USER fusion assembly standard===<br />
The Redoxilator: By in silico design and computer modelling followed by gene synthesis, we have constructed a molecular NAD/NADH ratio sensing system in Saccharomyces cerevisiae. The sensor works as an inducible transcription factor being active only at certain levels of the NAD/NADH ratios. By the coupling of a yeast optimized fast degradable GFP, the system can be used for in vivo monitoring of NAD/NADH redox poise. A future novel application of the system is heterologous redox coupled protein production in yeast. The USER fusion standard: Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER(TradeMark) cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any ‘scars’ from the restriction enzyme digestions.<br />
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===[[Team:Duke | Team Duke:]] One-Step Construction of a Bioplastic Production Pathway in E. coli===<br />
A convenient ligation-free, sequence-independent one-step plasmid assembly and cloning method is developed [Quan J, Tian J (2009) Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways. PLoS ONE 4(7): e6441]. The strategy, called Circular Polymerase Assembly Cloning (CPEC), relies on polymerase extension to assemble and clone multiple fragments into any vector. Using this method, we are able to quickly assemble a metabolic pathway consisting of multiple enzymes and regulatory elements for the production of a biocompatible as well as biodegradable plastic polymer in E. coli.<br />
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===[[Team:Edinburgh | Team Edinburgh:]] Defusing a dangerous world: a biological method for detection of landmines===<br />
Landmines left over from past conflicts are a major hazard in the world, killing and maiming many people every year. We have sought to engineer a bacterium able to detect TNT and its degradation products, nitrites, in the environment. Our system is based around a previously published computationally designed TNT-sensing protein derived from the periplasmic ribose binding protein, which interacts with an EnvZ-Trg transmembrane hybrid fusion protein and a nitrite-responsive repressor to trigger a pathway of TNT degradation and visualization using combined output from a bacterial luciferase and Yellow Fluorescent Protein. We envisage that the detection system could be applied by spraying the organism on soil where the presence of landmines is suspected, and detecting luminescence using low-light sensing. Once located, the mines could be safely removed. This system could be extended to detect other analytes in the environment.<br />
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===[[Team:EPF-Lausanne | Team EPF-Lausanne:]] E. Colight===<br />
Recent discoveries of photoreceptors in many organisms have given us insights into the interest of using light-responsive genetic tools in synthetic biology. The final goal of our project is to induce a change in gene expression, more specifically to turn a gene on or off, in a living organism, in response to a light stimulus. For this we use light-sensitive DNA binding proteins (or light-sensitive proteins that activate DNA binding proteins) to convert a light input into a chosen output, for example fluorescence, through a reporter gene such as RFP. Demonstrating that the light-induced gene switch tool works in vivo would show that easier and faster tools can potentially be made available in several fields of biology, as such tools can induce more localized, more precise (time resolution and reversibility) and drastically faster genetic changes than currently used ones, thus allowing research to evolve even better.<br />
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===[[Team:Freiburg_bioware | Team Freiburg_bioware:]] Universal endonuclease – cutting edge technology===<br />
Gene technology is driven by the use of restriction endonucleases. Yet, constraints of limited sequence length and variation recognized by available restriction enzymes pose a major roadblock for synthetic biology. We developed the basis for universal restriction enzymes, primarily for routine cloning but also with potential for in-vivo applications. We use a nucleotide cleavage domain fused to a binding domain, which recognizes a programmable adapter that mediates DNA binding and thus cleavage. As adapter we use readily available modified oligonucleotides, as binding domain anticalins, and as cleavage domain FokI moieties engineered for heterodimerization and activity. For application, this universal enzyme has merely to be mixed with the sequence-specific oligonucleotide and the target DNA. Binding and release are addressed by thermocycling. We provide concepts for in-vivo applications by external adapter delivery and activity regulation by photo switching. Additionally, an argonaute protein is engineered towards a DNA endonuclease.<br />
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===[[Team:Freiburg_software | Team Freiburg_software:]] SynBioWave – A Collaborative Synthetic Biology Software Suite===<br />
Synthetic Biology, which aims at constructing whole new genomes, is pushed forward by many users and relies on the assembly of genetic elements to devices and later systems. The construction process needs to be transparent and even at final stages control at the basepair level is required. We are building a software environment enabling multiple distributed users to analyze and construct genetic parts and ultimately genomes with real-time communication. Our current version demonstrates the principle use as well as the power of the underlying Google Wave protocol for collaborative synthetic biology efforts. Many wave-robots with a manageable set of capabilities will divide and conquer the complex task of creating a genome in silico. The initial developments of 'SynBioWave' lay the ground for basic layout, calling and data exchange of wave-robots in a clear and open process, so that future robots can be added and shared easily<br />
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===[[Team:Gaston_Day_School | Team Gaston_Day_School:]] Development of a Red Fluorescent Nitrate Detector===<br />
Increasing levels of fertilizer required for mechanized farming can result in elevated nitrate levels in soil and groundwater. Due to contaminated food and water, humans are at risk for Methemoglobinemia caused by enterohepatic metabolism of nitrates into ammonia. This process also oxidizes the iron in hemoglobin, rendering it unable to carry oxygen. Infants in particular are susceptible to Methemoglobinemia, also known as “Blue Baby Syndrome”, when formula is reconstituted using contaminated water. In order to prevent Methemoglobinemia, it is essential to detect high concentrations of nitrates. Fnr-NarG is an aerobic mutation of the nitrogen-sensitive promoter NarG that was provided by Dr. Lindow at UC Berkeley. By combining Red Fluorescent Protein with an aerobic mutant strain of NarG, the creation of Red Fluorescent Nitrate Detector (RFND) is possible. RFND is economically efficient because of its ability to self-replicate.<br />
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===[[Team:Groningen | Team Groningen:]] Heavy metal scavengers with a vertical gas drive===<br />
Heavy metal pollution of water and sediment endangers human health and the environment. To battle this problem, a purification strategy was developed in which arsenic, zinc or copper are removed from metal-polluted water and sediment. In this approach Escherichia coli bacteria accumulate metal ions from solutions, after which they produce gas vesicles and start floating. This biological device encompasses two integrated systems: one for metal accumulation, the other for metal-induced buoyancy. The uptake and storage system consists of a metal transporter and metallothioneins (metal binding proteins). The buoyancy system is made up of a metal-induced promoter upstream of a gas vesicle gene cluster. This device can be changed to scavenge for any compound by altering the accumulation and the induction modules. The combination of both systems enables the efficient decontamination of polluted water and sediment in a biological manner. <br />
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===[[Team:Harvard | Team Harvard:]] Interspecies Optical Communication Between Bacteria and Yeast===<br />
Optical communication is central to interactions between many multicellular organisms. However, it is virtually unknown between unicellular organisms, much less between unicellular organisms of different kingdoms of life. Our team has constructed a system that allows for interspecies, bacteria-to-yeast optical communication. To permit bacteria to send an optical signal, we expressed in E. coli a red firefly luciferase under IPTG induction. In yeast, we used a yeast-two-hybrid-system based on the interaction between the red-light-sensitive Arabidopsis thaliana phytochrome PhyB and its interacting factor PIF3. Interaction between PhyB and PIF3 is induced by the red light from the bacteria, resulting in transcription of the lacZ gene. This is an excellent demonstration of the principles and potential of synthetic biology: this system not only allows for interspecies optical communication, but enables us to optically bridge a physically separated canonical lac operon using light as a trans-acting factor.<br />
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===[[Team:Heidelberg | Team Heidelberg:]] Spybricks - a starter kit for synthetic biology in mammalian cells===<br />
Mammalian synthetic biology has a huge potential, but it is in need of new standards and a systematic construction of comprehensive part libraries. Promoters are the fundamental elements of every synthetic biological system. We have developed and successfully applied two novel, in silico guided methods for the rational construction of synthetic promoters which respond only to predefined transcription factors. Thus, we have been able to create a library of promoters of different strength and inducibility. To characterize the promoters, we have developed standardized protocols for comparable measurements of promoter strength by either transient or stable transfection. These synthetic promoters can be used as “spybricks” which enable the construction of assays for simultaneously monitoring several pathways in a cell. However, the potential of synthetic promoters goes far beyond this application: e.g. in virotherapy, these promoters could be used for selective gene expression in target cells.<br />
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===[[Team:HKU-HKBU | Team HKU-HKBU:]] Biomotor===<br />
Much hope has been laid on nanorobots in their application in therapeutics in this era of catheters and minimally invasive surgery, but the problem remains that purely mechanical nanorobots lack sufficient locomotive power to perform their intended tasks. Our 'bio-motor' aims to breach this gap to bring a foundational advancement. In our model, Escherichia coli cells are engineered to specifically express streptavidin at pole(s), which allows cells to adhere in the same orientation to a microrotary motor through biotin-streptavidin interaction. Thus, with the propulsion generated by bacterial flagella, this synthetic device is capable to convert biological energy into mechanical work. Furthermore, the propulsion energy was programmed to be adjustable by controlling E.coli swimming speed, i.e. putting E.coli cheZ gene under the control of ptet. This technology has tremendous potential to be applied in various fields including biomedicine, bio-energy, and bioengineering.<br />
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===[[Team:HKUST | Team HKUST:]] SynBiological Bug Buster===<br />
We aim to engineer a novel yeast strain that can detect, attract and eliminate pests. This strain would serve as an environmental-friendly substitute for pesticides. The idea is demonstrated by constructing an odorant sensing module, coupled production of chemical attractant and production of pest-killing binary toxin in yeast to kill pests lured to the yeast culture. A chimera G-protein coupled receptor (GPCR) responsive to an odorant chemical is coupled to the yeast mating pathway that can be activated upon ligand binding. It leads to over-expression of an endogenous yeast transaminase that catalyzes a reaction to yield 2-phenylethanol. Constitutively expressed binary toxin in the yeasts would poison the attracted pests after their consumption, as tested by feeding drosophilae. In addition to being a pesticide substitute, this cheaply-maintained engineered yeast strain also serves as a research reagent to screen for GPCRs that bind to certain ligands.<br />
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===[[Team:IBB_Pune | Team IBB_Pune:]] Constructing multi-strain computational modules using Nucleotide and Protein mediated cell-cell signaling.===<br />
Building complex genetic circuits in a single cell becomes difficult due to the formidable task of co-transforming large nucleotide sequences in addition to the imposed metabolic burden on the cell. Can a complex system be divided into independent modules that reside in different cells and interact with each other using nucleotide and protein mediated cell-cell signalling to act as a single unit? We seek to address this problem using a three pronged approach. Firstly, we are trying to introduce natural competance genes into the biobrick framework which will act as nucleotide importers. We are also building a protein export system using the TAT dependent export pathway. Finally, we are attempting to construct a multi-state turing machine which is a compound, modular computational system that has independent, interacting states which applies the above principle. We hope that this approach overcomes the obstacles in building more complex and composite circuits.<br />
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===[[Team:IIT_Bombay_India | Team IIT_Bombay_India:]] Analysis of multiple feedback loops using Synthetic Biology===<br />
One of the major objectives of synthetic biology is to unveil the inherent design principles prevailing in biological circuits. Multiple feedback loops (having both positive and negative regulation) are highly prevalent in biological systems. The relevance of such a design in biological systems is unclear. Our team will use synthetic biology approaches to answer these questions. Our team comprises of nine undergraduates, 3 graduate students as student mentor and two faculty mentors, one each from biology and engineering background. The project specifically deals with the analysis of effect of single and multiple feedback loops on gene expression. This project will involve theoretical and experimental studies. We have designed synthetic constructs to mimic multiple feedbacks. The focus of our experimental work will be to visualize the effect of multiple feedback loops on the synthetic construct using single cell analysis. The project will provide insights into the roles of multiple feedback loops in biological systems.<br />
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===[[Team:IIT_Madras | Team IIT_Madras:]] PLASMID: Plasmid Locking Assembly for Sustaining Multiple Inserted DNA===<br />
Any episome introduced into the cell shows segregational asymmetry accompanied with differential growth rates in the absence and presence of episome leading to an overall loss of the episomal unit in the absence of any selective pressure. We have designed a versatile system which maintains any given plasmid DNA in E.coli by using user-defined selection pressures, limited only by the presence of a response element to said pressure, like most antibiotics, certain chemicals and physical conditions. Depending on this selection pressure, a custom plasmid retaining system can be designed and co-transformed with the plasmid of interest to maintain it. A similar system can be used to “lock” the function of a gene of interest, like a combination lock, which is unlocked only when the cultures are grown in a pre-determined order of selection pressures. In principle, using this locking system, multiple plasmids can be maintained using a single selection pressure. <br />
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===[[Team:Illinois | Team Illinois:]] Bacterial Decoder===<br />
The Illinois iGEM team has been working to engineer a decoder function within E. coli. Decoders are logic devices used frequently in low-level computer architecture. We are creating a 2 to 4 decoder, which takes two binary inputs to activate one of four outputs. Each output corresponds to a specific combination of the inputs. With the presence of lactose and arabinose, our Bacterial Decoder will express Green Fluorescent Protein. If only lactose is present, a different fluorescent protein will be expressed. This goes for the other two combinations as well (only arabinose, or neither sugars). To implement logic we use combinations of small non-coding RNAs and transcription factors. The system allows the next engineer to swap standard parts in and out to change the inputs and outputs. Our Bacterial Decoder can help sense for multiple environmental cues, having implications for medical diagnostics and environmental and water contaminant detection.<br />
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===[[Team:Illinois-Tools | Team Illinois-Tools:]] Interactive Metabolic Pathway Tools===<br />
Interactive Metabolic Pathway Tools (IMP Tools) is an open source, web based program that involves model-guided cellular engineering where new metabolic functions can be added to existing microorganisms. This program will assist in the design stage of synthetic biology research. IMP tools is written primarily in python using the Django web framework. It takes a user-defined input compound, output compound, and weighting scheme and determines the ideal pathway from the starting to the ending compound. Our program presents an exciting capability to help transform important processes in the world for applications ranging from bioremediation to biofuels.<br />
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===[[Team:Imperial College London | Team Imperial College London:]] The E.ncapsulator===<br />
For iGEM 2009 the Imperial College London team present you with The E.ncapsulator; a versatile manufacture and delivery platform by which therapeutics can be reliably targeted to the intestine. Our E.coli chassis progresses through a series of defined stages culminating in the production of a safe, inanimate pill. This sequential process involves drug production, self-encapsulation in a protective coating and genome deletion. The temporal transition through each of these stages has been individually optimised by both media and temperature. The E.ncapsulator provides an innovative method to deliver any biologically synthesisable compound and bypasses the need for expensive storage, packaging and purification processes. The E.ncapsulator is an attractive candidate for commercial pill development and demonstrates the massive manufacturing potential in Synthetic Biology.<br />
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===[[Team:IPN-UNAM-Mexico | Team IPN-UNAM-Mexico:]] Turing meets synthetic biology: self-emerging patterns in an activator-inhibitor network.===<br />
We present a synthetic network that emulates an activator-inhibitor system. Our goal is to show that spatio-temporal structures can be generated by the behavior of a genetic regulatory network. We implement the model by means of several biobricks. We construct a self activating module and correspondingly an inhibitory one. Self-activation dynamics is given by the las operon, while the inhibitory part is provided by the lux operon. Quorum sensing and diffusion of AHL provide the reaction-diffusion mechanism responsible for the formation of Turing patterns. The importance of our work relies on the fact that we show that the action of the morphogenes as originally proposed by Turing is equivalent to the effect of diffusion of chemicals interacting with the synthetic network, which accounts for the reactive part, a possibility implicit in Turing’s original work in the context of morphogenesis of biological patterns.<br />
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===[[Team:IPOC1-Colombia | Team IPOC1-Colombia:]] Molecular Device to Detect Sea Salinity===<br />
Different gene parts are being assembled in order to construct a device that is able to detect different salinity levels in the sea. The device is tested against different concentrations of sodium chloride, fluoride, calcium, and magnesium. Different parameters, such as reporter fluorescence, DNA concentration, growth of bacterial device will be used to measure the efficacy of the device. Computational modeling will be used in the project to complement the laboratory work. Importance of project: Colombia borders two oceans: the Atlantic and the Pacific.<br />
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===[[Team:IPOC2-Colombia | Team IPOC2-Colombia:]] Molecular Device that Biodegrades Pesticides===<br />
Different gene parts are being assembled, in order to construct a device that is able to mineralize and biodegrade recalcitrant pesticides. The device will be tested against different concentrations of different recalcitrant pesticides. Specific chassis will be assembled with gene parts from different metabolic pathways in order to finally reach mineralization of the pesticide. Different parameters, such as DNA concentration, ATP concetration, fluorescence of reporters, growth of bacterial device, and reduction of pesticide concentration, will be used to assess the efficacy of the device. Computational modeling will be used in order to complement the laboratory work.<br />
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===[[Team:Johns_Hopkins-BAG | Team Johns_Hopkins-BAG:]] Synthetic yeast genome Sc2.0 and Build-A-Genome===<br />
The JHU team will present the work of the Build-A-Genome course, powering the fabrication of synthetic yeast genome Sc2.0. Build-A-Genome provides students tools to meld seamless arrays of DNA into predesigned synthetic chromosomes. Our team develops new technologies for synthetic genomic fabrication. We developed a new standard, the Building Block, allowing production of much longer DNA sequences that can encode for more complicated cellular operations than allowed by current iGEM biobrick standards, as well as more standard iGEM-y devices. Through multiple rounds of homologous recombination we can create chromosome segments and eventually full chromosomes. We will present our improved methodology for building block synthesis, the software we have created to aid in our synthesis, applications of the yeast genome redesign and the new standard we have created. We will emphasize the Build-A-Genome course and its implications on future genomic technologies that both rely on and teach students.<br />
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===[[Team:KU_Seoul | Team KU_Seoul:]] Integrated Heavy Metal Detection System ===<br />
Our team project is designing synthetic modules for simultaneous detection of multiple heavy metals such as arsenic, zinc, and cadmium in E. coli. The ultimate goal is to build a micromachine sensing and determining of the concentration of heavy metals in a sample solution (e.g. the waste water). In order to design the system, we will employ two fluorescence proteins (GFP and RFP) and aryl acylamidase as signal reporters. Since each heavy metal promoter produces unique fluorescence or color by those reporters, if more than two heavy metals coexist in a solution, the results would be interpreted from the convoluted fluorescence and/or color rather than a single signal detection. The successful construction of the synthetic modules in E. coli can be utilized in the form of a lyophilized powder, which can be stored in a drug capsule to make it portable.<br />
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===[[Team:KULeuven | Team KULeuven:]] Essencia coli, the fragrance factory===<br />
'Essencia coli' is a vanillin producing bacterium equipped with a control system that keeps the concentration of vanillin at a constant level. The showpiece of the project is the feedback mechanism. Vanillin synthesis is initiated by irradiation with blue light. The preferred concentration can be modulated using the intensity of that light. At the same time the bacterium measures the amount of vanillin outside the cell and controls its production to maintain the set point. The designed system is universal in nature and has therefore potential benefits in different areas. The concept can easily be applied to other flavours and odours. In fact, any application that requires a constant concentration of a molecular substance is possible.<br />
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===[[Team:Kyoto | Team Kyoto:]] Time Bomb & Cells in cells===<br />
We have two projects. The first is “GSDD”, the project to make a "time bomb"---a unique system to control the time of cell death. We create timer mechanism by taking advantage of the end-replication problem and the protecting effect of lacI (bound to each end of DNA) against exonuclease digestion. As the cell divides, due to the end-replication problem, the "timer" DNA gets shorter, and eventually, the repressor expression level falls. Then the downstream killer gene becomes expressed. The other one, “Cells in Cells” is the project to make a cell. We defined making cells as making liposomes that can divide like mitochondria do. To approach our goal, we set two subgoals. One is to enable cells to take in liposomes. The other is to enable the liposomes to import proteins needed for mitochondrial division. We suppose this could be the first step to create artificial cells.<br />
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===[[Team:LCG-UNAM-Mexico | Team LCG-UNAM-Mexico:]] Fight fire with fire: phage mediated bacterial bite back===<br />
Bacteriophage infection represents a common matter in science and industry. We propose to contend with such infections at a population level by triggering a defense system delivered by an engineered P4 phage. P4 is a satellite of P2 phage, so it cannot assembly unless some P2 genes are present. Those indispensable genes will be expressed by an E.Coli strain, hence creating a production line of a P4 which will be able to deliver (transduce) standardized synthetic systems to E. Coli and possibly similar species. The defense system will consist of toxins for DNA and rRNA degradation, transcribed by T3 or T7 RNA-Polymerases, fast enough to stop phage's assembly and scattering. The system includes the spread of an AHL, hence "warning" the population to prepare against further T3 or T7 infection. We will implement a stochastic population model to simulate the infection processes and quantify the efficiency of our system.<br />
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===[[Team:Lethbridge | Team Lethbridge:]] A Synthetic Future: Microcompartments, Nanoparticles and the BioBattery===<br />
The issues surrounding energy production are becoming more prominent with increasing environmental concerns and the rising cost of energy. Microbial fuel cells (MFCs) use biological systems to produce an electrical current. Cyanobacteria are organisms which have been studied in MFCs and have been found to create a current, although not highly efficient (Tsujimura et al., 2001). We wish to increase the efficiency of the cyanobacteria MFC by introducing microcompartments to create a BioBattery. The microcompartments are created by the production of the protein lumazine synthase forms icosahedral capsids. As a proof of principle we will create this system within Escherichia coli and target two different fluorescent proteins within the microcompartment to observe fluorescence resonance energy transfer. Furthermore, we will be exploring a novel method for the mass production of uniform nanoparticles, which is more efficient and cost effective than current methods.<br />
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===[[Team:McGill | Team McGill:]] Activation‐inactivation signaling in one‐and two‐dimensions===<br />
Intercellular signaling constitutes the foundation of may disparate research fields such as neurophysiology, embryology, cancer research, and several others. We investigated a simple representational intercellular signaling network where a population of cells synthesizes and secretes an activator molecule capable of activation a second population of cells into synthesizing and secreting an inhibitor molecular which feeds back and inhibits the production of the activating molecule. This is known as an activation-inhibition system. We began by using a partial differential equation model of the system to explore the effect of varying the separation distance of the two populations of cells. We found that three types of dynamics were present: steady states, periodic oscillations, and quasi-periodic oscillations. We further designed two strains of E. coli capable of interacting with each other as an activation-inhibition system and endeavored to validate our modeling results in a biological system.<br />
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===[[Team:METU-Gene | Team METU-Gene:]] A Fast Healing Mechanism; Wound Dressing===<br />
In case of bulk loss of tissue or non-healing wounds such as burns, trauma, diabetic, decubitus and venous stasis ulcers, a proper wound dressing is needed to cover the wound area, protect the damaged tissue, and if possible to activate the cell proliferation and stimulate the healing process. By this purpose, designing a wound dressing which is natural, non-toxic, and biodegradable and imitating the actual wound healing mechanism which is forming on open wounds in mammalian tissues is our main purpose.By this wound covering, we will fasten the healing process, and protect the wounded area from infectious agents. In this wound dressing, there will be 4 layers including polyurethane layers and our bacteria colonies. Our bacteria colonies will be capable of synthesizing human epidermal growth factor and keratinocyte growth factor. The communication between these bacteria colonies will be dependent on quorum sensing molecules.<br />
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===[[Team:Michigan | Team Michigan:]] The Toluene Terminator===<br />
Toluene is a toxic substance used in petrol, paint, paint-thinners and adhesives. Through spills and improper disposal, toluene can contaminate soil and ground water environments. Using microorganisms to clean up toluene-contaminated sites can be an effective and economical way of degrading the pollution before it can spread throughout the environment. There is concern, however, that these non-native microorganisms may upset the balance of the ecosystem through unnatural competition or horizontal synthetic gene transfer. We are engineering the Toluene Terminator as a way to neutralize toluene pollution while addressing these concerns. It will have the capabilities of sensing and mineralizing the toluene into carbon dioxide and water, but this terminator will not be back. The Toluene Terminator will have a suicide mechanism which kills the bacteria in the absence of toluene.<br />
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===[[Team:Minnesota | Team Minnesota:]] Computational synthetic biology: How the Synthetic Biology Software Suite can guide wet-lab experiments===<br />
Synthetic biology has all the characteristic features of an engineering discipline: applying technical and scientific knowledge to design and implement devices, systems, and processes that safely realize a desired objective. Mathematical modeling has always been an important component of engineering disciplines: models and computer simulations can quickly provide a clear picture of how different components influence the behavior of the whole, reaching objectives quickly. Our presentation focuses on sophisticated mathematical models of synthetic biological systems that connect the targeted biological phenotype to the DNA sequence. The activities for iGEM 2009 included the development and testing of simulation tools that connect multiple levels of organization from molecules and their interactions, to gene regulatory relations, to emerging logical architectures in bacteria. We connected out tools to the Registry and validated the simulations with a significant experimental component, constructing and testing these synthetic biological systems in Escherichia coli.<br />
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===[[Team:Missouri_Miners | Team Missouri_Miners:]] A Synthetic Biology Apporach to Microbial Fuel Cell Development Utilizing E. Coli===<br />
Optimization of electron shuffle to external surfaces such as anodes was a primary goal. Geobacter sulfurreducens happened to be our model bacteria due to its ability in nature to efficiently export electrons extracelluarly. E. coli was the chassis for this experiment due to its well documentation and the fact that its genome already containing some key proteins in our preferred pathway. The proteins, such as extracellular pilin, MacA, and many other cytochromes, which E. coli does not have were isolated from Geobacter sulfurreducens and introduced into E. coli to formulate the most optimal pathway for generating electromotive force in a microbial fuel cell apparatus. Some problems were faced concerning plasmid engineering and the simple fact that Geobacter is anaerobic and E. coli is aerobic. The current work includes production and optimization of a microbial fuel cell into which our modified bacteria will be placed.<br />
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===[[Team:MIT | Team MIT:]] Photolocalizer===<br />
There has been growing interest in designing fast and reversible switchable controls over all steps of gene expression, from transcription to post-translational modification. Our project involves engineering S. cerevisiae to localize proteins to various points in the cell in response to light exposure. Under red light, a tagged protein of interest localizes to a specific target, while exposure to far-red light causes the protein to rapidly delocalize and diffuse throughout the cell. This is accomplished using the PhyB-PIF3 system, a light-based transcriptional regulation system found in Arabidopsis. This project has two components. 1) Metabolically engineering yeast to endogenously produce PCB, a tetrapyrrole necessary for system activation, and 2) adapting the PhyB-PIF3 system to localize proteins of interest to different targets in the cell. The versatility and applications for this system are vast, ranging from cellular diffusion studies to easily synchronizing cell division for entire populations.<br />
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===[[Team:MoWestern_Davidson | Team MoWestern_Davidson:]] Rolling Clones: Can’t get no SATisfaction===<br />
Our team goal was to advance the developing field of bacterial computing. The Satisfiability (SAT) problem was the first mathematical problem proven to be NP-complete. A SAT problem is formed by connecting true-false variables with OR to form clauses and connecting clauses with AND. The goal is to determine if true-false values can be assigned to each variable to make the overall logical expression true. Our designed system uses frameshift suppressor tRNAs as inputs and frameshift suppressor leaders (FSLs) that process the inputs to enable the translation of fluorescent proteins exactly when an appropriate combination of inputs is present. The results illustrate the potential of engineered living cells to evaluate challenging mathematical problems. Our project also explored two aspects of synthetic biology education: a survey and analysis of public opinion and teachers’ knowledge of synthetic biology and the design and construction of physical models of a frameshift suppressor. <br />
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===[[Team:NCTU_Formosa | Team NCTU_Formosa:]] Bacterial referee with the adjustable timer and counter functions===<br />
Our team constructed a controllable synthetic genetic circuit in Escherichia coli which has timer and counter functions. The circuit works as an OR gate to integrate temporal and environmental signals. The output (red fluorescent protein: RFP) of the OR gate is ON when one of the input signals is ON. The timer function is controlled by Lac promoter, and the concentration of lactose determines timer’s working length. After added lactose is consumed by E. coli, the RFP will be translated to remind us that time’s up. The counter function can detect the bacteria population with LuxI/LuxR device; moreover, the counter sensitivity is controlled by the strength of TetR repressible promoter. If external bacteria invade the system, the extra AHL produced by them will induce the RFP translation to warn us the contamination. Our project can be applied to storage warning signs of fresh food, contact lens, and wound dressing.<br />
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===[[Team:Nevada | Team Nevada:]] Cinnamicide: Producing a Natural Insecticide against Mosquito Larvae in E. coli and Duckweed===<br />
Cinnamaldehyde is a natural insecticide against mosquito larvae that shows low toxicity towards other organisms. The objective of this project is to engineer the cinnamaldehyde biosynthetic pathway into E. coli to develop an inexpensive and readily available source of this compound. By introducing the genes encoding phenylalanine ammonia lyase, cinnamate-CoA ligase, and cinnamoyl-CoA reductase, it should be possible to produce cinnamaldehyde from available phenylalanine in E. coli. Once we have proven that we can produce cinnamaldehyde in E. coli, we will engineer cinnamaldehyde production in duckweed, a small aquatic plant. Because mosquito larvae feed on duckweed detritus, the engineered plant will serve as an excellent vehicle to deliver cinnamaldehyde for mosquito control.<br />
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===[[Team:Newcastle | Team Newcastle:]] Bac-man: sequestering cadmium into Bacillus spores===<br />
Cadmium contamination can be a serious problem in countries where polluting industries are located close to agricultural sites. Our team developed a design to address this problem using the resiliant spore-forming bacterium Bacillus subtilis. We engineered B. subtilis to sense and sequester cadmium from the environment into metallothionein containing spores, rendering it bio-unavailable. Germination of the spores can be disabled, making retrieval of the cadmium unnecessary since they can persist intact for thousands of years. We computationally simulated the life cycles of individual cells and entire cell populations, to estimate the parameter values necessary to maintain sustainable populations of sporulating, germinating and vegetative cells. Our design required us to engineer stochastic differentiation processes at a single cell level. A sporulation rate tuner was developed and we also engineered a tuneable stochastic invertase switch to stochastically control cell differention and fate. <br />
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===[[Team:NTU-Singapore | Team NTU-Singapore:]] Plaque Out!===<br />
The NTU iGEM ’09 team is proud to be working on a proposed alternative treatment for atherosclerosis. Our system, pLaqUe Out!, ideally based in a macrophage chassis, when introduced into the bloodstream, will be activated by a symptom typical of plaque buildup. Upon activation, our system will release a cholesterol degrading enzyme, a novel reporter protein and a vasodilator. The cholesterol degrading enzyme will break down the plaque. The reporter protein was chosen for expression because of its unique fluorescent property. This allows the plaque site to be identified in a manner similar to X-ray visualization. Finally the vasodilator will simultaneously dilate the blood vessels for better flow, as well as switch off the extended activity of our system. In the interests of time, this system is first modeled using E.Coli.<br />
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===[[Team:NYMU-Taipei | Team NYMU-Taipei:]] ViroCatcher===<br />
1. The objective: Binding viruses to designer ViroCatcher cells that cannot support viral replication to diagnose, attenuate, and prevent infection. 2. What we intend to do: (1) Make our designer cell safe, (2) Express specific cell surface receptors and antibodies to catch the virus, (3) Transduce the signal after viruses attached for feedback control, and (4) Remove the viruses along with ViroCatcher itself. 3. Anticipated results: the ViroCatcher is made safe for the bloodstream. When it is injected into the bloodstream, our ViroCatcher passively lies around letting viruses attach to it by using its 4 receptors: CD4 (for HIV), Integrin (for various viruses), Sialic Acid (for Influenza), and Antibodies (for Influenza). After enough viruses attach to it, or after a certain amount of time elapses, it removes itself from the bloodstream by calling macrophages to eat it up.<br />
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===[[Team:Osaka | Team Osaka:]] ColorColi: Painting tools toward bio-art===<br />
Bio-art has appeared as a crossover of life science and art in 21st century. Such artworks can give rise to a number of issues and metaphors accompanying the advance of science and technology. Astonishingly, there are still few collaborations between bio-art and synthetic biology. In this context, we engineered Salmonella enterica cells to function as new painting tools in bio-art. Specifically, we try to program cells to automatically form various pattern and gradation of colors by sensing cell identity and density by means of quorum sensing. Moreover, we will extend these tools for actual paintings and artworks to consider the ethical implications such as ’view of life’. This collaborative project can show the social situation or question of life science.<br />
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===[[Team:Paris | Team Paris:]] Message in a Bubble: a robust inter-cellular communication system based on outer membrane vesicles.===<br />
Sending a message across the ocean… Outer membrane vesicles (OMV), naturally produced by gram negative bacteria as E. Coli, are strong candidates for long-distance messaging. Our engineered communication platform consists of controlling OMV production by destabilizing membrane integrity through over-expression of specific periplasmic proteins (e.g., TolR). In order to control and modulate message content, we used fusions with OmpA signal sequence and the ClyA hemolysin as delivery tags. A targeting system was developed, based on the outer-membrane expression of Jun/Fos leucine zippers to control the vesicle flux between donor and recipient cells. Once received, the signal from incoming vesicles is transduced through a modified Fec pathway, whereby the receptor is provided by the OMV. Computational models provided insight to all of the above steps. Such reliable communications systems have wide biotechnological implications, ranging from targeted drugs delivery and detoxification to advanced division of labor or even cell-based computing.<br />
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===[[Team:PKU_Beijing | Team PKU_Beijing:]] Conditioned Reflex Mimicking in E.coli===<br />
We are engineering our E. coli cells to process the correlation information of two enviornmental signal, similar to the process of conditioning in higher orgamisms. We have constructed and tested a series of AND gates which can sense the two signals: the conditioned and unconditioned stimuli. With the presence of both signals, the AND gate outputs a repressor protein and then changes the state of the bistable switch, which acts as a memory module. In this way, our E. coli cells can convert the information about the concurrence of the two signals into its memory. After the memory module is switched and given the "conditioned stimulus", the E. coli cells will pass the information to the reporter module and thus exhibit the "conditioned response."<br />
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===[[Team:Purdue | Team Purdue:]] Engineered Microglia to Locate CD133+ Tumor-Initiating Cells===<br />
Glioblastoma multiforme (GBM) is one of the most common forms of primary brain cancer, which usually results in fatality. To date, it has been difficult to overcome primary brain cancer resulting from GBM, primarily because the cancer-initiating cells are suspected to be highly resistant to current cancer therapies. Specifically, CD133+ cells have shown resistance to hypoxia, irradiation, and some forms of chemotherapy. CD133+ hunting machines will be created by genetically engineering microglial cells (BV-2) with mammalian expression vectors. The project will also take advantage of inherent qualities of the microglia such as constant environmental sensing and quick motility. The engineered BV-2s will be equipped to locate the specific GBMs and label the targeted cells with a tat-GFP fusion protein. It is the goal of this study to show an alternative approach to cancer treatment, and to emphasize the power of biologically available options to fight the disease.<br />
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===[[Team:Queens | Team Queens:]] Plaque Busters: A Synthetic Biology Approach to Targeted Drug Delivery Treatment of Atherosclerosis===<br />
Atherosclerosis is associated with the buildup of plaques in the vascular walls. Currently, treatment for atherosclerosis involves preventative measures and surgical removal of plaque, angioplasty, and stent placement. We sought to develop an E. coli chassis delivering anti-atherosclerotic substances to the site of plaque in vasculature. Inflamed endothelial cells express VCAM-1, a receptor normally binds to the leukocyte antigen VLA-4. We attempted to express a VLA-4 fragment in E. coli, in order to selectively attach the cells to plaques. In vitro binding test uses inflamed murine endothelial cells which express VCAM-1. Results are pending. Our bacterial chassis also carries several inducible “effector” systems which, upon binding, release substances that facilitate plaque stabilization and regression. Effector systems include heme oxygenase-1, serum amyloid A and atrial natriuretic peptide. Expression of HO-1 in E. coli has been confirmed using spectroscopy. Testing for SAA secretion and ANP-induced gene expression in endothelial cells is ongoing. <br />
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===[[Team:SDU-Denmark | Team SDU-Denmark:]] Bacto Bandage - Quorum-quenching S. Aureus Biofilm Formation, One Peptide at a Time===<br />
Our goal is to create an E. coli strain, which inhibits Staphylococcus aureus biofilm formation in wounds by producing RNA III-inhibiting-peptide (RIP). S. aureus is one of the largest causes of hospital infections, each year infecting millions of people around the globe. S. aureus is normally commensal, but can create biofilms on implanted medical devices and in post-operational wounds. Biofilm is increasingly hard to treat, as a result of growing resistance to many types of antibiotics. By manipulating E. coli to express a synthetic RIP peptide tagged with an export signal, we hope to reach this goal. RIP has been shown to hinder the quorum-sensing processes essential for biofilm development in S. aureus, thereby making it harder for the bacteria to cause infections. We propose making a bandage that contains our engineered bacteria behind a semipermeable membrane, allowing only small peptides such as RIP to pass through, into the wound. <br />
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===[[Team:Sheffield | Team Sheffield:]] E. Coli Switch===<br />
By modifying E.coli so that it can use a phytochrome- with a light receptor- from cyanobacteria as a trigger of protein generation. This pathway is controlled by a certain wavelength of red light, acting as a system switch for lacZ production. LacZ can react with substrate X-gal and form a blue precipitate as a reporter. However, other reporter genes can be attached to the lacZ gene, so different reporters can be expressed. From the fact that this mechanism is sensitive to a certain wavelength of light, we hope to create a system that can be sensitive to various wavelengths and hence triggering different protein generation. Through this the E.coli can become a wavelength sensor; a different wavelength can trigger a different production of protein, for example various types of fluorescent protein, giving a different a colour-scaled indication of the wavelength of the environment around the E.coli.<br />
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===[[Team:SJTU-BioX-Shanghai | Team SJTU-BioX-Shanghai:]] Hypnos' Curse: E.coli the napper===<br />
Inspired by the natural regulator of circadian bioclock exhibited in most eukaryotic organisms, our team has designed an E.coli-based genetic network derived from the toxin-antitoxin system (TA system). The relE protein(toxin), is an RNase that preferentially cleaves mRNA stop codons, severely inhibiting translation and preventing colony formation. Whereas expression of relB protein(antitoxin) and tmRNA forms a rescue system to reverse inhibitory effects. Based on these mechanisms our network functions as a bacterial bioclock oscillating between the two states of dormancy and activity. Potential applications of our project include lifespan prolongation of prokaryotes and eukaryotes, since the metabolic process of microbes is vastly decelerated during the dormancy state, just like that of bears and hedgehogs in their hibernation.<br />
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===[[Team:Slovenia | Team Slovenia:]] nanoBRICKsPRO – synthetic smart nanomaterials from nano to macro===<br />
Nanotechnology designs materials with advanced properties based on the control of structure at the nanoscale. Biological systems provide an attractive opportunity to design and easily manufacture material with programmable properties. DNA origami demonstrated the power of this technology by creating a variety of assemblies that can be easily encoded in the nucleotide sequence. However, for biological nanodevices nature favors polypeptides over nucleic acids due to stability and versatility of amino-acid side chains. With few exceptions protein and peptide assemblies have been considered too difficult for the bottom-up design due to complex interactions and manufacturing problems specific for each case. We present technology for manufacturing nanomaterials based on combinations of modular peptide elements and protein domains, which allow self-assembly into complex tertiary structures with designed macroscopic properties. We will demonstrate the feasibility and potentials of protein nanotechnology by design, streamlining the production and technological application of nanomaterials based on nanoBRICKsPRO. <br />
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===[[Team:Southampton | Team Southampton:]] E.colYMPIC GAMES===<br />
The project exploits quorum sensing in E. coli to engineer interactions between 'species' such that complex spatiotemporal patterns are generated. We have two systems that correspond the game ³Rock, Paper, Scissors² (RPS) and to Conway¹s ³Game of Life² (GoL). In GoL expression/diffusion of lactones is exploited to create local rules that modulate expression of a fluorescent protein. Fluorescence patterns for different combinations of conditions are modelled using a new simulation tool designed to be generically applicable to inter-bacterial communication. In the RPS system three 'species' each produce a different fluorescent protein whose expression is downregulated by a lactone from one of the other 'species' and hence the interaction network is intransitive. Simulations indicate that when this game is played out on culture plates, a range of complex patterns evolve with time. Also, selective patterning of the different 'species' allows for new 'racetrack' or 'playing field' type of interactivity.<br />
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===[[Team:Stanford | Team Stanford:]] Immuni-T. coli: A Probiotic Approach to Diagnosing and Treating Inflammatory Bowel Disease (IBD)===<br />
Homeostasis relies on the balance between immune cell types, disruption of which leads to autoimmunity. The Stanford team has applied synthetic biology to a longstanding objective of immunotherapy: restoration and maintenance of homeostasis. Stanford’s Escherichia coli-based probiotic will polarize T cell differentiation along antagonistic fates - immunosuppressive Treg and inflammatory Th17 phenotypes - in response to local conditions. Our device design consists of two parts: one that modulates deleterious Treg-driven immunosuppression and another that engages Th17-mediated inflammation. Through specific sensors and effectors, therapy will oscillate between dampening pathologic inflammation and immunosuppression until a balance in the local T cell population is achieved. Securing such homeostasis between these populations has therapeutic implications for autoimmune disorders like IBD, HIV and cancer. We envision our novel and directed probiotic therapy for IBD as acting at the interface between commensal bacteria and human lymphocytes, integrating cutting-edge immunology with synthetic biology.<br />
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===[[Team:SupBiotech-Paris | Team SupBiotech-Paris:]] Double vectorisation system (DVS)===<br />
As a part of the iGEM competition, we decided to develop a new process, allowing the protection of the active biological principle. This type of process exists already, and it is called a vector. It may be biological as viruses, or chemicals as polymeric nanoparticles. Whatsoever its nature, the vector encounters many problems : Stability, targeting, membrane passage, and the immune response. Therefore, we tried to achieve the ideal vector, being the most stable as possible, can easily penetrate its targets, and outwit the immune system. We have created a double vectorization system, by using jointly a bacteria and a phage. The first vector which is bacterial, will target the tissue, and resist to the immune system. The second vector is a phage, will be used for cell targeting and membrane penetration. The combination of the two systems improves the intrinsic abilities of vectors, and offers new possibilities for applications.<br />
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===[[Team:Sweden | Team Sweden:]] The Linguistic Cell: Sentence Parsing Bacteria===<br />
Language is an essential part of our civilization. But making sense out of a series of words can only be achieved by certain rules that underlie the language. This set of rules is called a grammar. A grammar tells us how to order words in a meaningful way. These rules can be implemented as a Finite State Automaton (FSA), which for every new word input moves from the current state to the next until it reaches the end of input. We propose in our project a biological model which is based on this con- cept of language parsing from computational linguistics.<br />
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===[[Team:Tianjin | Team Tianjin:]] Cyanobacteria convertor & Microcystins detector===<br />
Project 1: Inspired by several features of cyanobacteria, which is low-grade, fast-growing, photosynthetic and easy to operate. We aim to construct an pathway in cyanobacteria so that when it is carrying photosynthesis,carbon dioxide can be transferred into target production, ethanol. Project 2: This project is to design a Yeast Two-hybrid system aimed at Microcystins(MCs) detection in waters. The MCs detection device we design takes the advantage of Gal4 promoter, which consists of two domains, one is AD, the other is BD. When AD and BD are close enough to each other, the report gene transcription LacZ will be trigged. We selected and modified two peptides that have specific interactions with MCs and engineered them into two vectors to construct the Yeast Two-hybrid system. In the presence of MCs at different concentrations, blue dots in different shades of colors can be seen directly. <br />
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===[[Team:Todai-Tokyo | Team Todai-Tokyo:]] Prevention of Lifestyle Diseases Using Synthetic Organisms===<br />
Lifestyle diseases, diseases caused by unhealthy living habits, comprise one of the major problems in modern society, especially as they may lead to fatal heart problems or even cancer. However, preventing or curing these diseases is presently of extreme difficulty. Our team, Todai-Tokyo, has been tackling treatment of lifestyle diseases such as hypercholesterolemia, diabetes, circadian rhythm dysfunction, and bad smoking habits by using synthetic living systems, utilizing their ability to incorporate complex logic functions and dispensability of external control once in operation. To do this, we aim to create the following: cells that ingest cholesterol to decrease blood cholesterol levels, healthy low calorie breads, a system in which periodic gene expression is controlled, and bacteria that encourage smokers to quit smoking, respectively. By applying similar synthetic biology methodologies to these, prevention of numerous lifestyle-related diseases may become reality, serving as a first step towards their eradication.<br />
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===[[Team:Tokyo_Tech | Team Tokyo_Tech:]] 2009 Space Odyssey: Terraforming of Mars with genetically engineered bacteria===<br />
Have any life forms existed on Mars? If so, what kind of features could they have possessed? Today, the Martian environment is severe for any life to inhabit because of some constrained conditions. For instances, the surface temperature having a range from -80℃ to 15℃, CO2 occupying 95% of the atmosphere and the absence of organic substances on the surface don't allow aerobic organisms or heterotrophic bacteria to grow. Our project objective is to create a genetically engineered iron-oxidizing bacteria surviving on Mars and to establish a new model organism playing an important role to terraform Mars. We engineered Acidithiobacillus ferrooxidans by introducing a synthetic pathway of both Melanin and Anti Freeze Protein with temperature-regulated systems. Anti Freeze Protein contributes to enhance tolerance of cryogenic condition and Melanin to blacken the Martian surface eventually resulting in melt of ice cap and generation of atmosphere and sea. <br />
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===[[Team:Tokyo-Nokogen | Team Tokyo-Nokogen:]] Escape tedious work with Escherichia coli Auto Protein Synthesizer (ESCAPES).===<br />
Tokyo-NokoGen has developed the Escherichia coli Auto Protein Synthesizer (ESCAPES), an E. coli machine that greatly simplifies the production of your favorite protein. We created a green light-activated actuator to respond to external light signals, as well as a riboregulator-based signal counter to count the number of flashes. In ESCAPES, the first green light flash induces the E. coli to self-aggregate, while the second flash causes them to auto-lyse, thus greatly simplifying the protein preparation process. The light-activated actuator was constructed by fusing the light responsive domain of the Synechocystis photoreceptor CcaS with the EnvZ histidine kinase domain. Self-aggregation is achieved by the induction of the Antigen43 gene, which we isolated from E. coli, while autolysis took advantage of the available BioBrick parts endolysin and holin. ESCAPES helps you “escape” from tedious protein preparation steps, such as centrifugation and cell disruption. <br />
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===[[Team:TorontoMaRSDiscovery | Team TorontoMaRSDiscovery:]] Engineering bacterial micro-compartments to investigate metabolic channeling and its potential uses in biotechnological applications===<br />
A key challenge in metabolic engineering is to improve productivity and yield. Potential applications range from the production of valuable compounds such as therapeutic molecules and biofuels to the degradation of toxic wastes. There is increasing recognition that spatial organization can play an important role in optimizing pathway efficiency. Specifically, the spatial co-localization of consecutive enzymes in a pathway can result in efficient translocation of substrates between enzymes, an effect known as enzyme "channeling". Here we report the design, modeling and construction of a bacterial micro-organelle based system for the targeted co-localization of selected enzymes. Our "Encapsulator" represents a fundamentally new class of parts which, in nature consist of metabolic enzymes encased within a multi-protein shell reminiscent of a viral capsid. Micro-compartments based on encapsulin (and similar proteins) represent an experimentally amenable system to investigate the effects of channeling in potential downstream applications.<br />
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===[[Team:Tsinghua | Team Tsinghua:]] Syn-genome Based Gensniper===<br />
Our aim is to construct a targeted gene therapy vector with high cellular specificity, considerable capacity and the potential for mass production and universal modification. Analogizing the characteristics of bacteriophage lambda and adenovirus, we genomically engineered the fiber protein of adenovirus with the pC of bacteriophage lambda, with the knob region modified by cell-specific peptides generated by phage display (called targeted biobrick). After inducing the vector genome (generated by bottom-up or top-down approach) into BL21 DE3 E.coli strain, we applied a co-transformed therapeutic DNA (namely a cosmid with a capacity of 40-50 kb) for mass production of our targeted gene therapy vectors containing the desired genes to be delivered. With the targeted biobrick immediating the attachment and RGD domain immediating the internalization of the targeted vector, we are able to accomplish the targeted gene therapy.<br />
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===[[Team:TUDelft | Team TUDelft:]] Bacterial Relay Race===<br />
In our project, we aim at creating a cell-to-cell communication system that allows the propagation of a set of instructions coded on a plasmid, and not just binary information as in quorum sensing. To achieve this goal, we have designed a communication system based on three different modules: a conjugation system, a time-delay genetic circuit, and a self- destructive plasmid. Cell-to-cell communication systems are important because, in most synthetic biology applications, the desired tasks are generally accomplished by a population of cells, rather than by a single cell. The proposed communication system could be used for creating a distributed sensors network, or it could help to better understand and possibly reduce antibiotic resistance in bacteria. Furthermore, we have conducted a survey to study the perception on synthetic biology and related ethical issues, among iGEM participants, students and supervisors. We have focused on the top-down and bottom-up approaches as applied to biology.<br />
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===[[Team:TzuChiU_Formosa | Team TzuChiU_Formosa:]] Midnight Apollo===<br />
In Taiwan there are 9 power stations generating energy by coal, and produce 269.1 million tons of CO2 every year. Power stations are major causes of global warming. Therefore, we would like to create a ” biolight” system that can reduce CO2 production and attenuate degree of global warming. We plan to create a new organism that doesn’t need electricity and cause no pollution. We named it “Midnight Apollo”. The “Midnight Apollo” will be turned on when surrounding area turns dark and will be turned off automatically when the environment becomes bright. The idea is based on two systems, Cph8 and aeoquorin/GFP. The Cph8 is regulated by visible light that can activate protein translation of an illuminating system. Subsequently, this illuminating system would use aeoquorin/GFP to light up the environment. We hope The Midnight Apollo could be applied in producing energy-saved Bio-streetlamp, emergency Biolighting, or Biosearchlight.<br />
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===[[Team:UAB-Barcelona | Team UAB-Barcelona:]] A toxics biosensor. Could bacteria detect instantaneous and simultaneously several types of pollutants?===<br />
We would like to construct a recombinant /Escherichia coli /strain that/ /could detect different aggressive pollutants, like toxics compounds. The first approach was the halogenated compounds (and more specifically chloroform) detector. Tap water usually contains it due to the chlorination process in drinking water production or other activities like swimming pools, etc. and it can become harmful to public health at high concentrations. /Nitrosomonas/ /europaea/’s promoters (/mbla/ and /clpb/) are specifically sensitive to chloroform, so coupling them with an output (a fluorescence protein) should allow quantifying its concentration. This was our first approximation towards our final aim of making a complete circuit that would allow to assign simultaneously to every pollutants family a certain color (fluorophore) thanks to the selective activation of different promoters.<br />
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===[[Team:UC_Davis | Team UC_Davis:]] A Bacterial Secretion System Motivated the Goal of Managing Celiac===<br />
The current estimate of the number of Americans with Celiac Disease/gluten intolerance is one out of 133. Not being able to digest gluten properly inside the small intestine leads to an immune system response that leads to a variety of symptoms. We have designed a bacterial secretion system that could be used in a probiotic organism to secrete an enzyme to degrade the allergen gliadin before it reaches the intestine. A putative advantage a probiotic treatment over direct enzyme therapy approaches is the potential for administering fewer doses, thus making it less troublesome, less costly, and more convenient. Our secretion system consists of an inducible promoter, ribosome binding site, an extracellular anchor (ompA/INPNC), cleavage signal sequence, 6 HIS Tag and a terminator. We have tested its behavior in E. coli on two proteins of varying sizes (GFP/Luciferase). <br />
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===[[Team:UChicago | Team UChicago:]] An enhanced yeast-based system for detection and decontamination of organophosphate neurotoxins.===<br />
Organophophates (OPs) are highly toxic compounds used as pesticides and chemical ware-fare agents around the world, including sarin, soman, and VX gas. To combat these toxins, which act as acetylcholinesterase inhibitors, we designed a highly efficient whole-cell S. cerevisiae sensor and biocatalyst system for the detection and remediation of the model organophosphate compound paraoxon and its degradation products. For our biosensor device, reporter constructs were incorporated into the genome downstream of paraoxon and paraoxon-hydrolyis sensitive promoters. Our degradation device was designed in three parts, targeting paraoxon through expression of organophosphate hydrolase from Flavobacterium, sp and two of its degradation byproducts p-nitrophenol and diethyl phosphate. Each device was genomically integrated in order to bypass selective the need for selective condition, while the degradation devices were constitutively expressed for maximum efficiency. Combined, this two-part system allows for both the detection and remediation of a broad range of common and deadly neurotoxins.<br />
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===[[Team:UCL_London | Team UCL_London:]] Stress Light===<br />
Our project “Stress Light” will produce a series of synthetic biosensor devises, which can improve on the traditional sensors in bio-processing; by using green fluorescent protein (GFP), yellow fluorescent protein (YFP), cyan fluorescent protein (CFP) and red fluorescent protein (RFP) expression as indicators of different stresses for e.coli bacteria during cultivation. Our product and system is called “the traffic-light stress sensor”. It is constructed to express fluorescent proteins of different colors in response to different stimuli which are inhibiting growth or harming production. We aim to build a sensor that can detect shear stress and low oxygen levels. We believe these two stresses are critical in a bioprocessing environment. We are applying e.coli’s native or modified promoters for DegP, Spy and NarK in order to induce transcription. Ideally the cells responses to stressed conditions should be sufficiently accurate, reliable and rapid for the stresses to be detected and mitigated.<br />
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===[[Team:UCSF | Team UCSF:]] Engineering the Movement of Cellular Robots===<br />
Some eukaryotic cells, such as white blood cells, have the amazing ability to sense specific external chemical signals, and move toward those signals. This behavior, known as chemotaxis, is a fundamental biological process crucial to such diverse functions as development, wound healing and immune response. Our project focuses on using a synthetic biology approach to manipulate signaling pathways that mediate chemotaxis. We are attempting to reprogram the movements that the cells undergo by altering the guidance and movement machinery of these cells in a modular way. For example, can we steer them to migrate toward new signals? Can we make cells move faster? Slower? We hope to better understand how chemotaxis works, and eventually build cells that can perform useful tasks. Imagine, for example, therapeutic cell-bots that could home to a directed site in the body and execute complex, user-defined functions (e.g., kill tumors, deliver drugs, guide stem cell migration). <br />
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===[[Team:ULB-Brussels | Team ULB-Brussels:]] GluColi, a new generation of glue===<br />
Whether you want to stop a leaking ship’s hull, or repair a fractured bone, you will need a top quality, strong adhesive. Our project aims to create a new generation of glue. In contrast to most glues, ours is natural, biodegradable, efficient on wet surfaces and has exceptional resistance (up to 3 times better than super glue). It is composed of a polysaccharide released by a Gram-negative bacterium, Caulobacter crescentus. Our aim is to use BioBrickTM standard biological parts in order to create a new strain of Escherichia coli which will synthesise this adhesive material. Moreover we are going to use a new plasmid stabilisation technique, the StabyTM system developed by Delphi Genetics. This system allows us to avoid the use of antibiotics and has been shown to be more efficient as far as protein secretion is concerned.<br />
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===[[Team:UNICAMP-Brazil | Team UNICAMP-Brazil:]] The Microguards===<br />
Industrial production processes based on microorganisms, such as biofuels, fine chemicals and enzymes, are threatened by contaminants that cause losses of up to 10% of the gross production. To solve this problem, the aim of our project is to engineer strains of the industrial workhorses E. coli and S. cerevisiae to recognize and destroy contaminants. The engineered yeast will recognize lactic acid bacteria contaminants, which greatly affect Brazilian´s ethanol production. The presence of lactic acid will activate a lysozyme-based killing mechanism, effective against gram-positive contamination. The engineered E. coli will recognize contaminants based on a non-self recognition mechanism and a percentage of the population will differentiate into a killing lineage releasing colicins and endonucleases. The killing mechanisms will be regulated by promoter Py, probably activated by conjugation. The characterization of Py and a lactate-inducible promoter that is not subjected to glucose catabolic repression are the main challenges of our project.<br />
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===[[Team:UNIPV-Pavia | Team UNIPV-Pavia:]] Ethanol? Whey not!===<br />
Cheese whey is classified as a special waste for its high biochemical and chemical oxygen demand. Even if whey can be valorized by extracting high value substances, like whey-proteins, at the end of the treatment the residual liquid is still a special waste for its high lactose content (4%). E. coli was engineered to convert efficiently lactose into ethanol, a precious biofuel. Three main enzymes are involved in this transformation: beta-galactosidase, pyruvate-decarboxylase and alcohol-dehydrogenaseII. Beta-galactosidase (lacZ gene) was over-expressed to obtain higher lactose-glucose conversion yield. Coding sequences of pyruvate-decarboxylase (pdc) and alcohol-dehydrogenaseII (adhB), essential in alcoholic fermentation pathway, were designed by DNA chemical synthesis and codon-optimized for E. coli. The final circuit includes the device to metabolize lactose and the ethanol-producing operon, containing pdc and adhB. It has a theoretical yield of 20kilos/tons of whey. Finally, 3OC6HSL, aTc and lactose/IPTG inducible systems were characterized to be used in this circuit.<br />
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===[[Team:uOttawa | Team uOttawa:]] A probiotic Lactobacillus strain which produces cellulose===<br />
This year’s project focuses on genetically engineering the bacterium Lactobacillus plantarum to produce cellulose, as a food additive. L. plantarum was selected as it is already commonly found in yogurt. The aim of generating this novel probiotic is to reduce human morbidity via the subsequent increase in dietary fibre in the gut. The sequestering of glucose for fibre production by L. plantarum provides the additional benefit of effectively reducing dietary sugars. We have successfully extracted the four genes that code for cellulose synthase from the Acetobacter xylinum. These genes were then placed under the control of a strong constitutive promoter, and transformed into Lactobacillus plantarum. Plasmid and genome based expression of the synthase genes are being evaluated and characterized. In the future, cellulose production assays, evaluation of biofilm formation, and in vivo testing will be performed to determine viability as potential health benefits.<br />
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===[[Team:Uppsala-Sweden | Team Uppsala-Sweden:]] Booze Bugs : Sun To Alcohol===<br />
In the long run our crude oil resources will be on the decline but most importantly the effects of the climate change demand a quick shift to a sustainable fuel economy. Approaching biofuel production by direct synthesis from sunlight has the potential to solve the problems that arise with the conventional fermentation of starches and sugars such as the direct competition of fuel feedstock with food crops. Thus the Uppsala iGEM Team 2009 investigated the production of ethanol and butanol with the use of the cyanobacteria Synechocystis sp PCC6803. Also known as blue-green algae, cyanobacteria possess the ability to directly convert sunlight into biofuels. We engineered constructs for ethanol and butanol production as well as strategies to increase the yields of photosynthetic ethanol production.<br />
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===[[Team:UQ-Australia | Team UQ-Australia:]] Mercury sequestration using a multicomponent operon, and increasing the temperature tolerance range of P. syringae.===<br />
Microbes such as Escherichia coli and Cuprivadis metallidurans have an endogenous multicomponent mercury (Hg2+) uptake and reduction operon, under the control of a metal responsive transcription factor, MerR. By utilising elements of this pathway, with a novel recovery mechanism, mercury can be accumulated intracellularly and efficiently removed from the environment. The presence of mercury activates MerR, driving the expression of Antigen 43 (Ag43), a self-adhering surface protein. Coupling a mercury sensitive promoter to the expression of Ag43 enables cells to accumulate mercury then aggregate in solution. P. syringae is a ubiquitous airborne bacterium which expresses a unique protein, InaZ. This protein acts as a scaffold for ice nucleation, inducing precipitation. Optimal growth of P. syringae occurs at 22oC. By introducing five heat-shock genes, the tolerance range will be increased to better suit the Australian climate. This modification has the potential to increase the availability of Australia’s most precious resource; water.<br />
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===[[Team:USTC | Team USTC:]] E. coli Automatic Directed Evolution Machine: a Universal Framework for Evolutionary Approaches in Synthetic Biology===<br />
Evolution is powerful enough to create everything, from biomolecules to ecosystems. The ultimate goal of E. coli Automatic Directed Evolution Machine (E.ADEM) project is to manage the power of evolution, by engineering a robust system framework that can automatically create anything we want in synthetic biology, from various types of parts to complex systems. Each demand can be converted into designing a scoring function to give the evolution process a direction. E.ADEM is designed by implementing evolutionary algorithm back into biology. The core of E.ADEM is a self-adaptive controller that can adjust variation rate and selection pressure, based on fitness score, population size and average fitness score calculated by a quorum sensing device. After comprehensive measurement using constitutive promoter family stimulus signals and modeling of the components, a prototype machine is built. Modular design and PoPS device boundary standard will ensure the extensibility and universality of the machine.<br />
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===[[Team:USTC_Software | Team USTC_Software:]] Automatic Biological Circuit Design===<br />
The ultimate goal of synthetic biology is to program complex biological networks that could achieve desired phenotype and produce significant metabolites in purpose of real world application, by fabricating standard components from an engineering-driven perspective. This project explores the application of theoretical approaches to automatically design synthetic complex biological networks with desired functions defined as dynamical behavior and input-output property. We propose a novel design scheme highlighted in the notion of trade-off that synthetic networks could be obtained by a compromise between performance and robustness. Moreover, series of eligible strategies, which consist of various topologies and possible standard components such as BioBricks, provide multiple choices to facilitate the wet experiment procedure. Description of all feasible solutions takes advantage of SBML and SBGN standard to guarantee extensibility and compatibility.<br />
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===[[Team:Utah_State | Team Utah_State:]] BioBricks without Borders: Investigating a multi-host BioBrick vector and secretion of cellular products===<br />
The aim of the Utah State University iGEM project is to develop improved upstream and downstream processing strategies for manufacturing cellular products using the standardized BioBrick system. First, we altered the broad-host range vector pRL1383a to comply with BioBrick standards and enable use of BioBrick constructs in organisms like Pseudomonas putida, Rhodobacter sphaeroides, and Synechocystis PCC6803. This vector will facilitate exploitation of advantageous characteristics of these organisms, such as photosynthetic carbon assimilation. Following expression, product recovery poses a difficult and expensive challenge. Downstream processing of cellular compounds, like polyhydroxyalkanoates (PHAs), commonly represents more than half of the total production expense. To counter this problem, secretion-promoting BioBrick devices were constructed through genetic fusion of signal peptides with protein-coding regions. To demonstrate this, the secretion of PHA granule-associated proteins and their affinity to PHA was investigated. Project success will facilitate expression and recovery of BioBrick-coded products in multiple organisms.<br />
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===[[Team:Valencia | Team Valencia:]] iLCD: iGEM Lighting Cell Display===<br />
The Valencia Team project consists of developing a “bio-screen” of voltage-activated cells, where every “cellular pixel” produces light. It is known that for instance neurons, cardiomyocites or muscle cells are able to sense and respond to electrical signals. These cells use a common second messenger system, calcium ion, which promotes a defined response when an electrical pulse is supplied to them. Nevertheless, these cultures present several technical disadvantages in order to make a handily use of them. Valencia team uses this property on yeast to produce luminescence as a response to electrical stimulus. This project constitutes the first time in which the electrical response of Saccharomyces and its potential applications are going to be tested. The obtained results will be used to build the first iLCD in history. We will reflect the perception that different groups of people have about Synthetic Biology in the survey http://igemvalencia.questionpro.com. <br />
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===[[Team:Victoria_Australia | Team Victoria_Australia:]] An environmentally sustainable biological lighting system ===<br />
Our aim is to build a biological lighting system via cell free transcription and translation. We will be focusing on developing a prototype using two cell free systems: E. coli and wheat germ in which the proteins will fluoresce. We are using the fluorescent proteins BFP, GFP, Vic green, blueberry, yellow and cherry in the cell free systems. Our main aim was to develop an alternative light source, which could possibly be powered by a waste material as simple as grass clippings (cell lysate). We are also attempting to develop a new registry part that is a blueberry fluorescent protein using the yellow protein (part # BBa_E0030) through mutagenesis. <br />
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===[[Team:VictoriaBC | Team VictoriaBC:]] Signal Integration: Applications of RNA Riboregulator Capabilities===<br />
This project explores some of the ways that the secondary structure of messenger RNA can be used to control the rate of protein expression. The 32<sup>o</sup>C ribothermometer made by the 2008 TUDelft team will be coupled to fluorescent proteins to visually confirm temperature-dependent translation. The "ribolock" made by the 2006 Berkeley team will be tested at various temperatures to determine if it could double for use as a ribothermometer. Finally, a proof-of-concept NAND logic gate will be constructed: a ribolock will be used to interpret two concurrent environmental signals into an on/off control for mCherry output.<br />
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===[[Team:Virginia | Team Virginia:]] Arsenic Sequestration for Groundwater Decontamination===<br />
As many as 137 million people in 70 countries may be affected by groundwater contaminated with arsenic. Existing treatment options are too expensive for the majority of affected areas. Therefore we are developing a bioremediation tool using Escherichia coli to absorb and bind arsenic and remove it from its surrounding environment. Natural and synthetic peptides are employed to sequester the toxic ions and a pump knockout ensures that arsenic stays in the cell. Measurement of growth capacity of the engineered strain in arsenic containing media and quantitative analysis of arsenic sequestration will be performed. Characterization and integration of an arsenic-responsive promoter will allow the sequestration system to dynamically adjust to current conditions. A simple, well-implemented system for biosequestration of arsenic may become part of a solution to a problem denying access to clean drinking water for many.<br />
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===[[Team:Virginia_Commonwealth | Team Virginia_Commonwealth:]] Promoter design, characterization and consequences===<br />
The generation of well-characterized genetic parts is a prerequisite for the rational design and construction of reliable genetically-encoded devices and systems. However, most publicly available parts (including those in the Registry) remain largely uncharacterized. Therefore, we propose a minimal measurement standard for the quantitative characterization of one of the most frequently used parts, promoters. This approach uses both mRNA and protein measurements to provide a tractable and universal analysis of relevant promoter characteristics. In an effort to elucidate promoter design principles, we have also designed and characterized new promoter and enhancer sequences. Our goal is to contribute to the advancement of fundamental synthetic biology by evaluating the performance of new and existing promoters and enhancers, which may serve as a model for describing other basic parts such as ribosome binding sites and transcriptional terminators.<br />
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===[[Team:Warsaw | Team Warsaw:]] BacInVader – a new system for cancer genetic therapy===<br />
The main aim of our project is to design a model system based on genetically modified Escherichia coli, capable of entering into eukaryotic cells. We have developed a regulatory system composed of three distinct functional modules. The whole system is activated by thermal degradation of the repressor protein, which leads to internalisation of E. coli by mammalian cells. When in endosome, pH-dependent two-component regulatory system activity enables the bacterium to escape to cytoplasm. Once the bacterium is in the cytoplasm some proteins are secreted due to expression of specific genes. In our case, secretion of p53 or bax proteins to mitochondria leads to apoptosis without cell cycle arrest thus enabling complementation of traditional chemotherapeutical agents, which affect only proliferating cells.<br />
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===[[Team:Wash_U | Team Wash_U:]] Improved Photosynthetic Productivity for Rhodobacter sphaeroides via Synthetic Regulation of the Light Harvesting Antenna LH2===<br />
Photosynthetic light harvesting antennas function to collect light and transfer energy to a reaction center for photochemistry. Phototrophs evolved large antennas to compete for photons in natural environments where light is scarce. Consequently, cells at the surface of photobioreactors over-absorb light, leading to attenuated photobioreactor light penetration and starving cells on the interior of photons. This reduction of photosynthetic productivity has been identified as the primary impediment to improving photobioreactor efficiency. While reduction of antenna size improves photosynthetic productivity, current approaches to this end uniformly truncate antennas and are difficult to manipulate from the perspective of bioengineering. We aim to create a modifiable system to optimize antenna size throughout the bioreactor by utilizing a synthetic regulatory mechanism that correlates expression of the pucB/A LH2 antenna genes with incident light intensity. This new application of synthetic biology serves to transform the science of antenna reduction into the engineering of antenna optimization. <br />
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===[[Team:Washington | Team Washington:]] The Ideal Protein Purification System===<br />
The use of recombinant protein production using E. coli-based expression systems has revolutionized the fields of biotechnology and medicine. However, the ability to utilize such proteins hinges upon their capacity to be isolated from their expression systems. Our project aims to create an all-in-one protein expression and purification system using BioBrick standards to greatly simplify protein production for synthetic biologists, reducing the time and cost involved in standard protein purification methods. Our method uses a novel combination of two systems: secretion and display. By fusing two tags to the protein it can be secreted into the expression media, and subsequently directed to bind to the outside of the cell. To collect the pure proteins, cells only need to be spun down and then resuspended in an elution buffer, releasing the protein of interest. Our research exhibits the utility of synthetic biology for developing new techniques that improve upon established practices.<br />
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===[[Team:Washington-Software | Team Washington-Software:]] LegoRoboBricks for Automated BioBrick Assembly===<br />
Commercial Liquid Handling Systems are extremely expensive, and are typically beyond the reach of the average molecular biologist interested in performing high throughput methods. To address this problem, we design and implement a liquid handling system built from commonly accessible Legos. Our goal is the automation of BioBrick assembly on a platform that can itself be easily replicated and we demonstrate a proof-of-principle for this system by transferring colored dye solutions on a 96-well plate. We introduce a new concept called LegoRoboBrick. The liquid handling system is build from 3 new LegoRoboBrick modular components: ALPHA (Automated Lego Pipette Head Assembly), BETA (BioBrick Environmental Testing Apparatus), and PHI (Pneumatic Handling Interface). We will demonstrate that the same BioBrick assembly software can run on multiple plug-and-play LegoRoboBrick instances with different physical dimensions and geometric configurations. The modular design of LegoRoboBricks allows easy extension of new laboratory functionalities in the future.<br />
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===[[Team:Waterloo | Team Waterloo:]] Chromobricks: A Platform for Chromosome Engineering with BioBricks===<br />
The aim of our project is to develop a fully-featured platform for chromosome engineering, allowing the in vivo assembly of a synthetic chromosome from interchangeable parts, followed by selective degradation of the native chromosome. We have designed a proof-of-concept for chromosome-building that will use the site-specific integrase of phage ΦC31 to integrate a BioBrick into a defined locus of the E. coli genome. Six pairs of integrase-targeted att sites have been designed to be non-cross-reactive in order to support repeatable cassette-exchange reactions for chromosome building. We have also written software to model the integrase-mediated rearrangement of DNA molecules containing att sites, to aid the design of more elaborate chromosome-building systems. To selectively degrade the native chromosome we designed a nuclease-based, inducible genome-degradation system. In its simplest form, our system can be used to integrate biological devices into a chromosome in situations requiring stable copy number and selection-free maintenance.<br />
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===[[Team:Wisconsin-Madison | Team Wisconsin-Madison:]] Ocean Fuel: increased salt tolerance through glycine betaine production===<br />
Biofuels represent a potential solution to current world energy demands. Total crude oil replacement based on a 20% fuel titer and current fuel demands would require 5.6 trillion gallons of fresh water per year. Current fresh water supplies may not support this added demand. Alternatively, a sustainable approach may use a portion of the Earth’s 3.5x1020 gallons of ocean water. However, current fuel-producing organisms are unable to thrive in ocean-level osmolarities. Glycine betaine, a powerful osmoprotectant, shields organisms from salt-induced stress. Wild-type Escherichia coli can acquire glycine betaine from their surroundings or synthesize it from environmental choline. Two enzymes, glycine/sarcosine methyltransferase, and sarcosine/dimethyglycine methyltransferase, catalyze three successive methylations of glycine for de novo synthesis of glycine betaine. Here, we demonstrate an engineered E. coli with an increased growth rate under salt induced stress. We highlight utility by demonstrating the improved growth of fuel producing bacteria in ocean water.<br />
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===[[Team:Yeshiva_NYC | Team Yeshiva_NYC:]] Spatially encoding temporal information: using diffusional escape of periplasmic reporter proteins as a clock.===<br />
We were inspired by iGEM projects that utilize colored or fluorescent reporter molecules. Specifically, we thought “wouldn’t it be nice to be able to leave a plate out in the field, collect it a day or two later, and be able to tell at what time the synthesis of the reporter molecules was triggered?” Ideally, protein reporters would leave the cell and diffuse through the agar, leaving a clear history of their expression. To this end, we are 1) creating a library of Sec and Tat leader sequence biobricks for directing expressed proteins to the periplasm, 2) making an E. coli expression strain with a weakened outer cell wall that leaks most of the periplasmic proteins to the environment, and 3) measuring and modeling the diffusion of small molecules and proteins in agar to ascertain the ability to derive the times and conditions under which they originated.</div>Meaganhttp://2009.igem.org/IGEM_PublicityIGEM Publicity2009-11-04T20:19:43Z<p>Meagan: </p>
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[https://2008.igem.org/IGEM_Publicity iGEM 2008 Publicity] | [http://parts.mit.edu/igem07/index.php/Media iGEM 2007 Publicity] | [http://parts.mit.edu/wiki/index.php/IGEM_News iGEM 2006 Publicity]<br />
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<span style="color:#1e90ff; font-size:175%">'''blogs </span><span style="color:#3cb371; font-size:175%">covering iGEM 2009'''</span><br />
* The [http://igem.sdu.dk/ <span style="color:#453221; font-size: 130%">'''SDU-Denmark'''</span>] team blog about their iGEM experience.<br />
* A blog about the iGEM project of the [http://aboutgmos.org/iGEM <span style="color:#453221; font-size: 130%">'''Uppsala-Sweden'''</span>] team.<br />
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====<font size=5><font color=dodgerblue>'''video/radio</font><font color=mediumseagreen> about iGEM 2009'''</font></font>====<br />
*'''Slovenia, Heidelberg, SDU-Denmark''': [http://www.dradio.de/dlf/sendungen/forschak/1062608/ Die Biobastler von Boston], Nov 2, 2009, Deutschlandfunk radio (in German).<br />
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====<font size=5><font color=dodgerblue>'''news articles</font><font color=mediumseagreen> about iGEM 2009'''</font></font>====<br />
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====<font size=4><font color=dodgerblue>'''general'''</font></font>====<br />
*'''The Economist''': ''Biohacking: Hacking goes squishy'' Sep 3, 2009 [http://www.economist.com/search/displaystory.cfm?story_id=14299634 The Economist]<br />
*'''The Scientist''': ''Brick by Brick'' Feb 1, 2009. [http://www.the-scientist.com/article/display/55378/ The Scientist]<br />
<br />
<br />
====<font size=4><font color=dodgerblue>'''team specific'''</font></font>====<br />
<br />
*'''[[Team:IBB_Pune]]''': "<i>Bio-champs in the making</i>" (English), [http://www.punemirror.in/index.aspx?Page=article&sectname=News%20-%20City&sectid=2&contentid=200909142009091423331646a131eacc#ftr2 (Monday, September 14, 2009 at 11:33:25 PM)]<br />
*'''[[Team:IBB_Pune]]''': "<i>Ganiti Prakriyanmadhe Jeevanuncha Vaapar! (Use of Bacteria in mathematical devices)</i>" (Marathi), [http://beta.esakal.com/2009/09/09220618/pune-use-of-bacterias-in-maths.html(Wednesday, September 09th, 2009 AT 10:09 PM)]<br />
*'''[[Team:IBB_Pune]]''': "<i>Synthetic Biologiteel Bharari ("Advances in Synthetic Biology")</i>" (Marathi), [https://2009.igem.org/Team:IBB_Pune/press (Wednesday 7 October 2009)]<br />
<br />
*'''[[Team:Groningen]]''': "<i>Synthetic biology students from Groningen in the race for best bacterium</i>" (Dutch), [http://www.cityoftalent.nl/nl/content/nieuws/nieuws/groningse-studenten-synthetische-biologie-maken-bacterie City of Talent (August 20, 2009)]<br />
<br />
*'''[[Team:SupBiotech-Paris]]''': "<i>Un concours organisé par le M.I.T : en route pour Boston ! </i>" (French), [http://www.supbiotech.fr/2009/09/boston-supbiotech-igem.html (September 08, 2009)]<br />
<br />
*'''[[Team:SupBiotech-Paris]]''': "<i>Les étudiants de la biotech interpellent la biologie synthétique</i>" (French), [http://www.vivagora.org/spip.php?breve210 (October 09, 2009)]<br />
<br />
*'''[[Team:UAB-Barcelona]]''': "<i>Un equipo de la UAB, en el concurso de biología sintética del MIT</i>" (Spanish), [http://www.uab.es/servlet/Satellite?cid=1096481466568&pagename=UABDivulga%2FPage%2FTemplatePageDetallArticleInvestigar&param1=1253860327385 (September 23, 2009)]<br />
<br />
*'''[[Team:UAB-Barcelona]]''': "<i>UAB to participate in the synthetic biology competition at MIT</i>" (English), [http://www.uab.es/servlet/Satellite/latest-news/news-detail/uab-to-participate-in-the-synthetic-biology-competition-at-mit-1096476786473.html?noticiaid=1253657853358 (September 23, 2009)]<br />
<br />
*'''[[Team:Valencia]]''': "<i>TheValencia team is awarded the Synthetic Standard Prize</i>" (Spanish), [http://www.elpais.com/articulo/sociedad/Levaduras/funcionan/pixeles/elpepuespval/20091103elpepusoc_9/Tes]</div>Meaganhttp://2009.igem.org/IGEM_PublicationsIGEM Publications2009-11-04T20:06:27Z<p>Meagan: </p>
<hr />
<div>'''[[Team:Valencia | Team Valencia]]:''' J. Delás, M. Notari, J. Forés, J. Pechuan, M. Porcar, E. Navarro, A. Montagud, M. Baguena, J. Peretó, P. Fernández de Córdoba, M.M. González-Barroso, E. Rial, A. Moya and J. Urchueguía (2009). [http://www.ncbi.nlm.nih.gov/pubmed/19761877 "Yeast cultures with UCP1 uncoupling activity as a heating device."] ''New Biotechnology, 2009 Sep 15.''</div>Meaganhttp://2009.igem.org/IGEM_PublicationsIGEM Publications2009-11-04T20:06:15Z<p>Meagan: </p>
<hr />
<div>'''[[Team:Valencia | Team Valencia]]:''' J. Delás, M. Notari, J. Forés, J. Pechuan, M. Porcar, E. Navarro, A. Montagud, M. Baguena, J. Peretó, P. Fernández de Córdoba, M.M. González-Barroso, E. Rial, A. Moya and J. Urchueguía (2009). [http://www.ncbi.nlm.nih.gov/pubmed/19761877 "Yeast cultures with UCP1 uncoupling activity as a heating device."] ''New Biotechnology,'' 2009 Sep 15.</div>Meaganhttp://2009.igem.org/IGEM_PublicationsIGEM Publications2009-11-04T20:05:47Z<p>Meagan: </p>
<hr />
<div>'''[[Team:Valencia | Team Valencia]]:''' J. Delás, M. Notari, J. Forés, J. Pechuan, M. Porcar, E. Navarro, A. Montagud, M. Baguena, J. Peretó, P. Fernández de Córdoba, M.M. González-Barroso, E. Rial, A. Moya and J. Urchueguía (2009). [http://www.ncbi.nlm.nih.gov/pubmed/19761877 "Yeast cultures with UCP1 uncoupling activity as a heating device."] ''New Biotechnology.'' In press.</div>Meaganhttp://2009.igem.org/IGEM_PublicationsIGEM Publications2009-11-04T20:04:22Z<p>Meagan: </p>
<hr />
<div>'''[[Team:Valencia | Team Valencia]]:''' J. Delás, M. Notari, J. Forés, J. Pechuan, M. Porcar, E. Navarro, A. Montagud, M. Baguena, J. Peretó, P. Fernández de Córdoba, M.M. González-Barroso, E. Rial, A. Moya and J. Urchueguía (2009). "Yeast cultures with UCP1 uncoupling activity as a heating device." ''New Biotechnology.'' In press.</div>Meaganhttp://2009.igem.org/Main_PageMain Page2009-11-04T19:51:04Z<p>Meagan: </p>
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<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> <a class="quick_link" href="https://2009.igem.org/Jamboree">iGEM 2009 Jamboree</a></h3><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center; font-weight:bold;">October 31 to November 2, 2009</p><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;">Massachusetts Institute of Technology</p><br />
<ul style="list-style:none;" ><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Quick links:</lh><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Project_Abstract/Team_Abstracts">Team abstracts</a></li><br />
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<li><a class="quick_link" href="https://igem.org/Results?year=2009">iGEM 2009 Jamboree results</li><br />
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<p style="font-size: 200%; font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#ed0100; text-align:center; margin-left:20px;">iGEM 2009 is officially over!</p><br />
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<li><strong>Grand Prize, Winner of the BioBrick Trophy:</strong> Cambridge</li><br />
<li><strong>1st Runner Up:</strong> Heidelberg</li><br />
<li><strong>2nd Runner Up:</strong> Valencia</li><br />
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<lh style="font-size:110%; font-weight:bold; color:#576f91;">Track Award Winners:</lh><br />
<li><strong>Best Food or Energy Project:</strong> UNIPV-Pavia</li><br />
<li><strong>Best Environment Project:</strong> Cambridge</li><br />
<li><strong>Best Health or Medicine Project:</strong> Stanford</li><br />
<li><strong>Best Manufacturing Project:</strong> Imperial College London</li><br />
<li><strong>Best New Application Area:</strong> Valencia</li><br />
<li><strong>Best Foundation Advance:</strong> Alberta</li><br />
<li><strong>Best Information Processing Project:</strong> TUDelft</li><br />
<li><strong>Best Software Tool:</strong> Berkeley Software & Illinois-Tools <small>(Tie)</small></li><br />
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<lh style="font-size:110%; font-weight:bold; color:#576f91;">Special Prizes Winners:</lh><br />
<li><strong>Best BioBrick Part, Natural:</strong> ULB-Brussels</li><br />
<li><strong>Best New BioBrick Part or Device, Engineered:</strong> EPF-Lausanne & Freiburg Bioware <small>(Tie)</small></li><br />
<li><strong>Best Human Practices Advance:</strong> Imperial College London & Paris <small>(Tie)</small></li><br />
<li><strong>Best Experimental Measurement:</strong> Valencia</li><br />
<li><strong>Best Model:</strong> BCCS-Bristol</li><br />
<li><strong>Best Wiki:</strong> Heidelberg</li><br />
<li><strong>Best Poster:</strong> Freiburg bioware</li><br />
<li><strong>Best Presentation:</strong> ArtScienceBangalore</li><br />
<li><strong>Best New Standard:</strong> Heidelberg</li><br />
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<img src="https://static.igem.org/mediawiki/2009/6/6c/Nsf_logo_h26px.jpg"<br />
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<td><br />
<a href="https://2009.igem.org/Sponsors"><br />
<img src="https://static.igem.org/mediawiki/2009/6/6f/BBF_h30px.png"<br />
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<a href="https://2009.igem.org/Sponsors"><br />
<img src="https://static.igem.org/mediawiki/2009/d/df/Sponsor_footer_trim.png"<br />
style="padding-left:10px;"<br />
alt="iGEM 2009 Sponsors"<br />
title="iGEM 2009 Sponsors"><br />
</a><br />
<a href="https://2009.igem.org/Sponsorship"><br />
<img src="https://static.igem.org/mediawiki/2009/8/8d/Sponsor_now_h35px.png"<br />
style="padding-left:10px;"<br />
alt="Become an iGEM sponsor"<br />
title="Become an iGEM sponsor"><br />
</a><br />
</div><br />
</html></div>Meaganhttp://2009.igem.org/File:Star_25.jpgFile:Star 25.jpg2009-11-04T17:00:28Z<p>Meagan: </p>
<hr />
<div></div>Meaganhttp://2009.igem.org/File:Star_50.jpgFile:Star 50.jpg2009-11-04T16:48:58Z<p>Meagan: </p>
<hr />
<div></div>Meaganhttp://2009.igem.org/Main_PageMain Page2009-11-04T16:22:35Z<p>Meagan: </p>
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alt="Main page image: freezer plates"<br />
title="Repository freezer plates."><br />
<img src="https://static.igem.org/mediawiki/2009/3/3d/Slovenia_mascot_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: Slovenia mascot."<br />
title="iGEM 2008 Slovenia mascot."><br />
<img src="https://static.igem.org/mediawiki/2009/5/57/Igem_banner_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: iGEM banner." <br />
title="iGEM banner."> <br />
<!-- <img src="https://static.igem.org/mediawiki/2009/3/37/Finalist_announce_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: 2008 finalist announcement"<br />
title="Announcing the finalists at the 2008 Jamboree."> --><br />
<!-- <img src="https://static.igem.org/mediawiki/2009/2/23/Dhl_boxes_2_xsmall.jpg"<br />
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title="DHL boxes for the DNA distribution."> --><br />
<img src="https://static.igem.org/mediawiki/2009/3/38/Blackboard_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: Tagging blackboard!"<br />
title="Tagging the iGEM blackboard!"><br />
<img src="https://static.igem.org/mediawiki/2009/3/3a/Phage_mascot_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: Heidelberg phage mascot"<br />
title="iGEM 2008 Heidelberg phage mascot."><br />
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<img src="https://static.igem.org/mediawiki/2009/a/ab/Kit_plates_xsmall.jpg"<br />
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title="DNA distribution kit plates."><br />
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<div id="news_items" style="width: 700px; margin-left: 20px; float:left;"> <br />
<br />
<!-- *** QUICK LINKS BOX *** --><br />
<div id="jamboree_highlight_box" style="border:3px solid #fed801; width:700px; margin:0px 0px 30px 0px; padding:5px; background-color:#fbefb8;"><br />
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<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> <a class="quick_link" href="https://2009.igem.org/Jamboree">iGEM 2009 Jamboree</a></h3><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center; font-weight:bold;">October 31 to November 2, 2009</p><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;">Massachusetts Institute of Technology</p><br />
<ul style="list-style:none;" ><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Quick links:</lh><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Project_Abstract/Team_Abstracts">Team abstracts</a></li><br />
<li><a class="quick_link" href="https://igem.org/Team_Wikis?year=2009">Team websites</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Schedule">Schedule</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Campus_map">Campus Map</a></li><br />
<li><a class="quick_link" href="https://igem.org/Results?year=2009">iGEM 2009 Jamboree results</li><br />
</ul><br />
</td><br />
</td><br />
<td style="width:325px;"><br />
<img src="https://static.igem.org/mediawiki/2009/9/95/2009_igem_from_above_300.jpg"<br />
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<!-- *** JAMBOREE RESULTS *** --><br />
<div id="jamboree_results_box" style="width:700px; margin:0px 0px 30px 0px;"><br />
<p style="font-size: 200%; font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#ed0100; text-align:center; margin-left:20px;">iGEM 2009 is officially over!</p><br />
<img src="https://static.igem.org/mediawiki/2009/2/2a/Jamboree2009_cambridge_award.jpg"<br />
style="margin-left:125px; border:2px solid #aaa"><br />
<br />
<!-- GRAND PRIZE, 1ST, 2ND --><br />
<ul style="list-style:none; font-size:110%; color:#666; font-family: Lucida Grande, Verdana, Arial, sans-serif; text-align:center;"><br />
<li><strong>Grand Prize, Winner of the BioBrick Trophy:</strong> Cambridge</li><br />
<li><strong>1st Runner Up:</strong> Heidelberg</li><br />
<li><strong>2nd Runner Up:</strong> Valencia</li><br />
</ul><br />
<br />
<!-- FINALISTS --><br />
<div id="finalist_list"><br />
<ul style="list-style: none;"><br />
<lh>Finalists:</lh><br />
<li>Cambridge</li><br />
<li>Freiburg bioware</li><br />
<li>Groningen</li><br />
<li>Heidelberg</li><br />
<li>Imperial College London</li><br />
<li>Valencia</li><br />
</ul><br />
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<!-- TRACK AND SPECIAL AWARDS --><br />
<table style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666;"><br />
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<ul style="list-style:none;"><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Track Award Winners:</lh><br />
<li><strong>Best Food or Energy Project:</strong> UNIPV-Pavia</li><br />
<li><strong>Best Environment Project:</strong> Cambridge</li><br />
<li><strong>Best Health or Medicine Project:</strong> Stanford</li><br />
<li><strong>Best Manufacturing Project:</strong> Imperial College London</li><br />
<li><strong>Best New Application Area:</strong> Valencia</li><br />
<li><strong>Best Foundation Advance:</strong> Alberta</li><br />
<li><strong>Best Information Processing Project:</strong> TUDelft</li><br />
<li><strong>Best Software Tool:</strong> Berkeley Software & Illinois-Tools <small>(Tie)</small></li><br />
</ul><br />
</td><br />
<td style="width:350px;"><br />
<ul style="list-style:none;"><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Special Prizes Winners:</lh><br />
<li><strong>Best BioBrick Part, Natural:</strong> ULB-Brussels</li><br />
<li><strong>Best New BioBrick Part or Device, Engineered:</strong> EPF-Lausanne & Freiburg Bioware <small>(Tie)</small></li><br />
<li><strong>Best Human Practices Advance:</strong> Imperial College London & Paris <small>(Tie)</small></li><br />
<li><strong>Best Experimental Measurement:</strong> Valencia</li><br />
<li><strong>Best Model:</strong> BCCS-Bristol</li><br />
<li><strong>Best Wiki:</strong> Heidelberg</li><br />
<li><strong>Best Poster:</strong> Freiburg bioware</li><br />
<li><strong>Best Presentation:</strong> ArtScienceBangalore</li><br />
<li><strong>Best New Standard:</strong> Heidelberg</li><br />
</td><br />
</tr><br />
</table><br />
<p style="font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> See the <a href="https://igem.org/Results?year=2009">Results page</a> for details</p><br />
</div> <!-- close Jamboree results box --><br />
<br />
<br />
<br />
<!-- ***** Begin entry style portion of page ***** --><br />
<br />
<br />
<br />
<br />
<!-- *** ARCHIVE *** --><br />
<a href="https://2009.igem.org/Main_page_archive"><small>(News post archives)</small></a><br />
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<div class='right-heading'>About iGEM</div><br />
* [[About | What is iGEM?]]<br />
<!--* [[iGEM_Publicity | News about iGEM]]--><br />
* [[Previous iGEM Competitions | Previous iGEM competitions]]<br />
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<div class='right-heading'>iGEM Start to Finish</div><br />
* [[Calendar of Events | Calendar of events]]<br />
* [[Start_A_Team | Start a team]]<br />
* [[Requirements]]<br />
* [https://igem.org/IGEM_2009_Registration iGEM 2009 Registration]<br />
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* [[Jamboree | The Jamboree]]<br />
** [[Jamboree/Attendance fee | Registration]]<br />
** [[Jamboree/Schedule | Schedule]]<br />
** [[Judging | Judging]]<br />
* <span style="font-weight:bold; font-size:115%;">[[Photo_Gallery | iGEM Photo Gallery]]</span><br />
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* [[Security]]<br />
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<!--* [[Mailing_Lists | Mailing lists]]--><br />
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* [https://2009.igem.org/Team_Wikis?year=2009 2009 team wikis]<br />
* [https://igem.org/Team_Parts?year=2009 2009 team parts]<br />
* [https://igem.org/Team_Judging?year=2009 2009 judging forms]<br />
* [[Partner_Offers | iGEM Partner Offers]]<br />
* [[Instructional Videos]]<br />
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* [[Main page archive | Main page archive]]<br />
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style="padding-left:10px;"<br />
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</html></div>Meaganhttp://2009.igem.org/Main_PageMain Page2009-11-03T20:07:30Z<p>Meagan: </p>
<hr />
<div>__NOTOC__<br />
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alt="Main page image: freezer plates"<br />
title="Repository freezer plates."><br />
<img src="https://static.igem.org/mediawiki/2009/3/3d/Slovenia_mascot_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: Slovenia mascot."<br />
title="iGEM 2008 Slovenia mascot."><br />
<img src="https://static.igem.org/mediawiki/2009/5/57/Igem_banner_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
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<!-- <img src="https://static.igem.org/mediawiki/2009/3/37/Finalist_announce_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: 2008 finalist announcement"<br />
title="Announcing the finalists at the 2008 Jamboree."> --><br />
<!-- <img src="https://static.igem.org/mediawiki/2009/2/23/Dhl_boxes_2_xsmall.jpg"<br />
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alt="Main page image: DHL boxes"<br />
title="DHL boxes for the DNA distribution."> --><br />
<img src="https://static.igem.org/mediawiki/2009/3/38/Blackboard_xsmall.jpg"<br />
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<!-- *** QUICK LINKS BOX *** --><br />
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<table style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; background-color:#fbefb8;"><br />
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<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> <a class="quick_link" href="https://2009.igem.org/Jamboree">iGEM 2009 Jamboree</a></h3><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center; font-weight:bold;">October 31 to November 2, 2009</p><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;">Massachusetts Institute of Technology</p><br />
<ul style="list-style:none;" ><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Quick links:</lh><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Project_Abstract/Team_Abstracts">Team abstracts</a></li><br />
<li><a class="quick_link" href="https://igem.org/Team_Wikis?year=2009">Team websites</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Schedule">Schedule</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Campus_map">Campus Map</a></li><br />
<li><a class="quick_link" href="https://igem.org/Results?year=2009">iGEM 2009 Jamboree results</li><br />
</ul><br />
</td><br />
</td><br />
<td style="width:325px;"><br />
<a href="https://2009.igem.org/Image:2009_iGEM_from_Above_highres.jpg"><br />
<img src="https://static.igem.org/mediawiki/2009/9/95/2009_igem_from_above_300.jpg"<br />
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<!-- *** JAMBOREE RESULTS *** --><br />
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<p style="font-size: 200%; font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#ed0100; text-align:center; margin-left:20px;">iGEM 2009 is officially over!</p><br />
<img src="https://static.igem.org/mediawiki/2009/2/2a/Jamboree2009_cambridge_award.jpg"<br />
style="margin-left:125px; border:2px solid #aaa"><br />
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<!-- GRAND PRIZE, 1ST, 2ND --><br />
<ul style="list-style:none; font-size:110%; color:#666; font-family: Lucida Grande, Verdana, Arial, sans-serif; text-align:center;"><br />
<li><strong>Grand Prize, Winner of the BioBrick Trophy:</strong> Cambridge</li><br />
<li><strong>1st Runner Up:</strong> Heidelberg</li><br />
<li><strong>2nd Runner Up:</strong> Valencia</li><br />
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<!-- FINALISTS --><br />
<div id="finalist_list"><br />
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<lh>Finalists:</lh><br />
<li>Cambridge</li><br />
<li>Freiburg bioware</li><br />
<li>Groningen</li><br />
<li>Heidelberg</li><br />
<li>Imperial College London</li><br />
<li>Valencia</li><br />
</ul><br />
</div> <!-- close finalists --><br />
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<!-- TRACK AND SPECIAL AWARDS --><br />
<table style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666;"><br />
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<ul style="list-style:none;"><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Track Award Winners:</lh><br />
<li><strong>Best Food or Energy Project:</strong> UNIPV-Pavia</li><br />
<li><strong>Best Environment Project:</strong> Cambridge</li><br />
<li><strong>Best Health or Medicine Project:</strong> Stanford</li><br />
<li><strong>Best Manufacturing Project:</strong> Imperial College London</li><br />
<li><strong>Best New Application Area:</strong> Valencia</li><br />
<li><strong>Best Foundation Advance:</strong> Alberta</li><br />
<li><strong>Best Information Processing Project:</strong> TUDelft</li><br />
<li><strong>Best Software Tool:</strong> Berkeley Software & Illinois-Tools <small>(Tie)</small></li><br />
</ul><br />
</td><br />
<td style="width:350px;"><br />
<ul style="list-style:none;"><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Special Prizes Winners:</lh><br />
<li><strong>Best BioBrick Part, Natural:</strong> ULB-Brussels</li><br />
<li><strong>Best New BioBrick Part or Device, Engineered:</strong> EPF-Lausanne & Freiburg Bioware <small>(Tie)</small></li><br />
<li><strong>Best Human Practices Advance:</strong> Imperial College London & Paris <small>(Tie)</small></li><br />
<li><strong>Best Experimental Measurement:</strong> Valencia</li><br />
<li><strong>Best Model:</strong> BCCS-Bristol</li><br />
<li><strong>Best Wiki:</strong> Heidelberg</li><br />
<li><strong>Best Poster:</strong> Freiburg bioware</li><br />
<li><strong>Best Presentation:</strong> ArtScienceBangalore</li><br />
<li><strong>Best New Standard:</strong> Heidelberg</li><br />
</td><br />
</tr><br />
</table><br />
<p style="font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> See the <a href="https://igem.org/Results?year=2009">Results page</a> for details</p><br />
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* [[Calendar of Events | Calendar of events]]<br />
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** [[Jamboree/Attendance fee | Registration]]<br />
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* [https://igem.org/Team_Parts?year=2009 2009 team parts]<br />
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style="padding-left:10px;"<br />
alt="Become an iGEM sponsor"<br />
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</html></div>Meaganhttp://2009.igem.org/IGEM_PublicityIGEM Publicity2009-11-03T20:05:45Z<p>Meagan: </p>
<hr />
<div>__NOTOC__<br />
[https://2008.igem.org/IGEM_Publicity iGEM 2008 Publicity] | [http://parts.mit.edu/igem07/index.php/Media iGEM 2007 Publicity] | [http://parts.mit.edu/wiki/index.php/IGEM_News iGEM 2006 Publicity]<br />
<br />
<div style="color:#aaa; padding-bottom:20px;">(Members of the press, please see the [[Press_Kit | iGEM Press Kit]])</div><br />
<br />
<br />
<span style="color:#1e90ff; font-size:175%">'''blogs </span><span style="color:#3cb371; font-size:175%">covering iGEM 2009'''</span><br />
* The [http://igem.sdu.dk/ <span style="color:#453221; font-size: 130%">'''SDU-Denmark'''</span>] team blog about their iGEM experience.<br />
* A blog about the iGEM project of the [http://aboutgmos.org/iGEM <span style="color:#453221; font-size: 130%">'''Uppsala-Sweden'''</span>] team.<br />
<br />
<br />
<br />
<br />
====<font size=5><font color=dodgerblue>'''video/radio</font><font color=mediumseagreen> about iGEM 2009'''</font></font>====<br />
*'''Slovenia, Heidelberg, SDU-Denmark''': [http://www.dradio.de/dlf/sendungen/forschak/1062608/ Die Biobastler von Boston], Nov 2, 2009, Deutschlandfunk radio (in German).<br />
<br />
<br />
<br />
<br />
====<font size=5><font color=dodgerblue>'''news articles</font><font color=mediumseagreen> about iGEM 2009'''</font></font>====<br />
<br />
<br />
If you would like to share an article that was written about iGEM or your iGEM team, please link to it on this page. If you have multiple articles featuring your team, link to them all individually!<br />
<br />
Post the name of your team, the title of your article, where it was featured, and provide a link to it. <br />
<br />
''Example'':<br> <br />
'''Team Example''': ''Title of article'', Nature, [link]<br />
<br />
====<font size=4><font color=dodgerblue>'''general'''</font></font>====<br />
*'''The Economist''': ''Biohacking: Hacking goes squishy'' Sep 3, 2009 [http://www.economist.com/search/displaystory.cfm?story_id=14299634 The Economist]<br />
*'''The Scientist''': ''Brick by Brick'' Feb 1, 2009. [http://www.the-scientist.com/article/display/55378/ The Scientist]<br />
<br />
<br />
====<font size=4><font color=dodgerblue>'''team specific'''</font></font>====<br />
<br />
*'''[[Team:IBB_Pune]]''': "<i>Bio-champs in the making</i>" (English), [http://www.punemirror.in/index.aspx?Page=article&sectname=News%20-%20City&sectid=2&contentid=200909142009091423331646a131eacc#ftr2 (Monday, September 14, 2009 at 11:33:25 PM)]<br />
*'''[[Team:IBB_Pune]]''': "<i>Ganiti Prakriyanmadhe Jeevanuncha Vaapar! (Use of Bacteria in mathematical devices)</i>" (Marathi), [http://beta.esakal.com/2009/09/09220618/pune-use-of-bacterias-in-maths.html(Wednesday, September 09th, 2009 AT 10:09 PM)]<br />
*'''[[Team:IBB_Pune]]''': "<i>Synthetic Biologiteel Bharari ("Advances in Synthetic Biology")</i>" (Marathi), [https://2009.igem.org/Team:IBB_Pune/press (Wednesday 7 October 2009)]<br />
<br />
*'''[[Team:Groningen]]''': "<i>Synthetic biology students from Groningen in the race for best bacterium</i>" (Dutch), [http://www.cityoftalent.nl/nl/content/nieuws/nieuws/groningse-studenten-synthetische-biologie-maken-bacterie City of Talent (August 20, 2009)]<br />
<br />
*'''[[Team:SupBiotech-Paris]]''': "<i>Un concours organisé par le M.I.T : en route pour Boston ! </i>" (French), [http://www.supbiotech.fr/2009/09/boston-supbiotech-igem.html (September 08, 2009)]<br />
<br />
*'''[[Team:SupBiotech-Paris]]''': "<i>Les étudiants de la biotech interpellent la biologie synthétique</i>" (French), [http://www.vivagora.org/spip.php?breve210 (October 09, 2009)]<br />
<br />
*'''[[Team:UAB-Barcelona]]''': "<i>Un equipo de la UAB, en el concurso de biología sintética del MIT</i>" (Spanish), [http://www.uab.es/servlet/Satellite?cid=1096481466568&pagename=UABDivulga%2FPage%2FTemplatePageDetallArticleInvestigar&param1=1253860327385 (September 23, 2009)]<br />
<br />
*'''[[Team:UAB-Barcelona]]''': "<i>UAB to participate in the synthetic biology competition at MIT</i>" (English), [http://www.uab.es/servlet/Satellite/latest-news/news-detail/uab-to-participate-in-the-synthetic-biology-competition-at-mit-1096476786473.html?noticiaid=1253657853358 (September 23, 2009)]</div>Meaganhttp://2009.igem.org/IGEM_PublicityIGEM Publicity2009-11-03T20:04:45Z<p>Meagan: </p>
<hr />
<div>__NOTOC__<br />
[https://2008.igem.org/IGEM_Publicity iGEM 2008 Publicity] | [http://parts.mit.edu/igem07/index.php/Media iGEM 2007 Publicity] | [http://parts.mit.edu/wiki/index.php/IGEM_News iGEM 2006 Publicity]<br />
<br />
<div style="color:#aaa; padding-bottom:20px;">(Members of the press, please see the [[Press_Kit | iGEM Press Kit]])</div><br />
<br />
<br />
<span style="color:#1e90ff; font-size:175%">'''blogs </span><span style="color:#3cb371; font-size:175%">covering iGEM 2009'''</span><br />
* The [http://igem.sdu.dk/ <span style="color:#453221; font-size: 130%">'''SDU-Denmark'''</span>] team blog about their iGEM experience.<br />
* A blog about the iGEM project of the [http://aboutgmos.org/iGEM <span style="color:#453221; font-size: 130%">'''Uppsala-Sweden'''</span>] team.<br />
<br />
<br />
====<font size=5><font color=dodgerblue>'''videos</font><font color=mediumseagreen> about iGEM 2009'''</font></font>====<br />
*'''Slovenia, Heidelberg, SDU-Denmark''': [http://www.dradio.de/dlf/sendungen/forschak/1062608/ Die Biobastler von Boston], Nov 2, 2009, Deutschlandfunk radio (in German).<br />
<br />
<br />
====<font size=5><font color=dodgerblue>'''news articles</font><font color=mediumseagreen> about iGEM 2009'''</font></font>====<br />
<br />
<br />
If you would like to share an article that was written about iGEM or your iGEM team, please link to it on this page. If you have multiple articles featuring your team, link to them all individually!<br />
<br />
Post the name of your team, the title of your article, where it was featured, and provide a link to it. <br />
<br />
''Example'':<br> <br />
'''Team Example''': ''Title of article'', Nature, [link]<br />
<br />
====<font size=4><font color=dodgerblue>'''general'''</font></font>====<br />
*'''The Economist''': ''Biohacking: Hacking goes squishy'' Sep 3, 2009 [http://www.economist.com/search/displaystory.cfm?story_id=14299634 The Economist]<br />
*'''The Scientist''': ''Brick by Brick'' Feb 1, 2009. [http://www.the-scientist.com/article/display/55378/ The Scientist]<br />
<br />
<br />
====<font size=4><font color=dodgerblue>'''team specific'''</font></font>====<br />
<br />
*'''[[Team:IBB_Pune]]''': "<i>Bio-champs in the making</i>" (English), [http://www.punemirror.in/index.aspx?Page=article&sectname=News%20-%20City&sectid=2&contentid=200909142009091423331646a131eacc#ftr2 (Monday, September 14, 2009 at 11:33:25 PM)]<br />
*'''[[Team:IBB_Pune]]''': "<i>Ganiti Prakriyanmadhe Jeevanuncha Vaapar! (Use of Bacteria in mathematical devices)</i>" (Marathi), [http://beta.esakal.com/2009/09/09220618/pune-use-of-bacterias-in-maths.html(Wednesday, September 09th, 2009 AT 10:09 PM)]<br />
*'''[[Team:IBB_Pune]]''': "<i>Synthetic Biologiteel Bharari ("Advances in Synthetic Biology")</i>" (Marathi), [https://2009.igem.org/Team:IBB_Pune/press (Wednesday 7 October 2009)]<br />
<br />
*'''[[Team:Groningen]]''': "<i>Synthetic biology students from Groningen in the race for best bacterium</i>" (Dutch), [http://www.cityoftalent.nl/nl/content/nieuws/nieuws/groningse-studenten-synthetische-biologie-maken-bacterie City of Talent (August 20, 2009)]<br />
<br />
*'''[[Team:SupBiotech-Paris]]''': "<i>Un concours organisé par le M.I.T : en route pour Boston ! </i>" (French), [http://www.supbiotech.fr/2009/09/boston-supbiotech-igem.html (September 08, 2009)]<br />
<br />
*'''[[Team:SupBiotech-Paris]]''': "<i>Les étudiants de la biotech interpellent la biologie synthétique</i>" (French), [http://www.vivagora.org/spip.php?breve210 (October 09, 2009)]<br />
<br />
*'''[[Team:UAB-Barcelona]]''': "<i>Un equipo de la UAB, en el concurso de biología sintética del MIT</i>" (Spanish), [http://www.uab.es/servlet/Satellite?cid=1096481466568&pagename=UABDivulga%2FPage%2FTemplatePageDetallArticleInvestigar&param1=1253860327385 (September 23, 2009)]<br />
<br />
*'''[[Team:UAB-Barcelona]]''': "<i>UAB to participate in the synthetic biology competition at MIT</i>" (English), [http://www.uab.es/servlet/Satellite/latest-news/news-detail/uab-to-participate-in-the-synthetic-biology-competition-at-mit-1096476786473.html?noticiaid=1253657853358 (September 23, 2009)]</div>Meaganhttp://2009.igem.org/Main_PageMain Page2009-11-03T19:55:30Z<p>Meagan: </p>
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title="iGEM 2008 Slovenia mascot."><br />
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<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> <a class="quick_link" href="https://2009.igem.org/Jamboree">iGEM 2009 Jamboree</a></h3><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center; font-weight:bold;">October 31 to November 2, 2009</p><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;">Massachusetts Institute of Technology</p><br />
<ul style="list-style:none;" ><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Quick links:</lh><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Project_Abstract/Team_Abstracts">Team abstracts</a></li><br />
<li><a class="quick_link" href="https://igem.org/Team_Wikis?year=2009">Team websites</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Schedule">Schedule</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Campus_map">Campus Map</a></li><br />
<li><a class="quick_link" href="https://igem.org/Results?year=2009">iGEM 2009 Jamboree results</li><br />
</ul><br />
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<a href="https://2009.igem.org/Image:2009_iGEM_from_Above_highres.jpg"><br />
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<!-- *** JAMBOREE RESULTS *** --><br />
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<p style="font-size: 200%; font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#ed0100; text-align:center; margin-left:20px;">iGEM 2009 is officially over!</p><br />
<img src="https://static.igem.org/mediawiki/2009/2/2a/Jamboree2009_cambridge_award.jpg"<br />
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<li><strong>Grand Prize, Winner of the BioBrick Trophy:</strong> Cambridge</li><br />
<li><strong>1st Runner Up:</strong> Heidelberg</li><br />
<li><strong>2nd Runner Up:</strong> Valencia</li><br />
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<lh>Finalists:</lh><br />
<li>Cambridge</li><br />
<li>Freiburg bioware</li><br />
<li>Groningen</li><br />
<li>Heidelberg</li><br />
<li>Imperial College London</li><br />
<li>Valencia</li><br />
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<lh style="font-size:110%; font-weight:bold; color:#576f91;">Track Award Winners:</lh><br />
<li><strong>Best Food or Energy Project:</strong> UNIPV-Pavia</li><br />
<li><strong>Best Environment Project:</strong> Cambridge</li><br />
<li><strong>Best Health or Medicine Project:</strong> Stanford</li><br />
<li><strong>Best Manufacturing Project:</strong> Imperial College London</li><br />
<li><strong>Best New Application Area:</strong> Valencia</li><br />
<li><strong>Best Foundation Advance:</strong> Alberta</li><br />
<li><strong>Best Information Processing Project:</strong> TUDelft</li><br />
<li><strong>Best Software Tool:</strong> Berkeley Software & Illinois-Tools <small>(Tie)</small></li><br />
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<lh style="font-size:110%; font-weight:bold; color:#576f91;">Special Prizes Winners:</lh><br />
<li><strong>Best BioBrick Part, Natural:</strong> ULB-Brussels</li><br />
<li><strong>Best New BioBrick Part or Device, Engineered:</strong> EPF-Lausanne & Freiburg Bioware <small>(Tie)</small></li><br />
<li><strong>Best Human Practices Advance:</strong> Imperial College London & Paris <small>(Tie)</small></li><br />
<li><strong>Best Experimental Measurement:</strong> Valencia</li><br />
<li><strong>Best Model:</strong> BCCS-Bristol</li><br />
<li><strong>Best Wiki:</strong> Heidelberg</li><br />
<li><strong>Best Poster:</strong> Freiburg bioware</li><br />
<li><strong>Best Presentation:</strong> ArtScienceBangalore</li><br />
<li><strong>Best New Standard:</strong> Heidelberg</li><br />
</td><br />
</tr><br />
</table><br />
<p style="font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> See the <a href="https://igem.org/Results?year=2009">Results page</a> for details</p><br />
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</html></div>Meaganhttp://2009.igem.org/Main_PageMain Page2009-11-03T19:27:13Z<p>Meagan: </p>
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<img src="https://static.igem.org/mediawiki/2009/3/3d/Slovenia_mascot_xsmall.jpg"<br />
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title="iGEM 2008 Slovenia mascot."><br />
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style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
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title="iGEM banner."> <br />
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<img src="https://static.igem.org/mediawiki/2009/3/38/Blackboard_xsmall.jpg"<br />
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alt="Main page image: Heidelberg phage mascot"<br />
title="iGEM 2008 Heidelberg phage mascot."><br />
<!-- <img src="https://static.igem.org/mediawiki/2009/9/9e/Presentation_room_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: 2008 Jamboree presentation room"<br />
title="2008 Jamboree presentation room."> --><br />
<img src="https://static.igem.org/mediawiki/2009/a/ab/Kit_plates_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: DNA distribution kit plates"<br />
title="DNA distribution kit plates."><br />
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<!-- *** QUICK LINKS BOX *** --><br />
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<td style="width:375px;"><br />
<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> <a class="quick_link" href="https://2009.igem.org/Jamboree">iGEM 2009 Jamboree</a></h3><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center; font-weight:bold;">October 31 to November 2, 2009</p><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;">Massachusetts Institute of Technology</p><br />
<ul style="list-style:none;" ><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Quick links:</lh><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Project_Abstract/Team_Abstracts">Team abstracts</a></li><br />
<li><a class="quick_link" href="https://igem.org/Team_Wikis?year=2009">Team websites</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Schedule">Schedule</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Campus_map">Campus Map</a></li><br />
<li class="pending_results">iGEM 2009 Jamboree results</li><br />
</ul><br />
</td><br />
</td><br />
<td style="width:325px;"><br />
<a href="https://2009.igem.org/Image:2009_iGEM_from_Above_highres.jpg"><br />
<img src="https://static.igem.org/mediawiki/2009/9/95/2009_igem_from_above_300.jpg"<br />
style="margin-right:-110px;"><br />
</a><br />
</td><br />
</tr><br />
</table><br />
</div><br />
<br />
<!-- *** JAMBOREE RESULTS *** --><br />
<div id="jamboree_results_box" style="width:700px; margin:0px 0px 30px 0px;"><br />
<p style="font-size: 200%; font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#ed0100; text-align:center; margin-left:20px;">iGEM 2009 is officially over!</p><br />
<img src="https://static.igem.org/mediawiki/2009/2/2a/Jamboree2009_cambridge_award.jpg"<br />
style="margin-left:125px; border:2px solid #aaa"><br />
<br />
<!-- GRAND PRIZE, 1ST, 2ND --><br />
<ul style="list-style:none; font-size:110%; color:#666; font-family: Lucida Grande, Verdana, Arial, sans-serif; text-align:center;"><br />
<li><strong>Grand Prize, Winner of the BioBrick Trophy:</strong> Cambridge</li><br />
<li><strong>1st Runner Up:</strong> Heidelberg</li><br />
<li><strong>2nd Runner Up:</strong> Valencia</li><br />
</ul><br />
<br />
<!-- FINALISTS --><br />
<div id="finalist_list"><br />
<ul style="list-style: none;"><br />
<lh>Finalists:</lh><br />
<li>Cambridge</li><br />
<li>Freiburg bioware</li><br />
<li>Groningen</li><br />
<li>Heidelberg</li><br />
<li>Imperial College London</li><br />
<li>Valencia</li><br />
</ul><br />
</div> <!-- close finalists --><br />
<br />
<!-- TRACK AND SPECIAL AWARDS --><br />
<table style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666;"><br />
<tr><br />
<td style="width:350px;"><br />
<ul style="list-style:none;"><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Track Award Winners:</lh><br />
<li><strong>Best Food or Energy Project:</strong> UNIPV-Pavia</li><br />
<li><strong>Best Environment Project:</strong> Cambridge</li><br />
<li><strong>Best Health or Medicine Project:</strong> Stanford</li><br />
<li><strong>Best Manufacturing Project:</strong> Imperial College London</li><br />
<li><strong>Best New Application Area:</strong> Valencia</li><br />
<li><strong>Best Foundation Advance:</strong> Alberta</li><br />
<li><strong>Best Information Processing Project:</strong> TUDelft</li><br />
<li><strong>Best Software Tool:</strong> Berkeley Software & Illinois-Tools <small>(Tie)</small></li><br />
</ul><br />
</td><br />
<td style="width:350px;"><br />
<ul style="list-style:none;"><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Special Prizes Winners:</lh><br />
<li><strong>Best BioBrick Part, Natural:</strong> ULB-Brussels</li><br />
<li><strong>Best New BioBrick Part or Device, Engineered:</strong> EPF-Lausanne & Freiburg Bioware <small>(Tie)</small></li><br />
<li><strong>Best Human Practices Advance:</strong> Imperial College London & Paris <small>(Tie)</small></li><br />
<li><strong>Best Experimental Measurement:</strong> Valencia</li><br />
<li><strong>Best Model:</strong> BCCS-Bristol</li><br />
<li><strong>Best Wiki:</strong> Heidelberg</li><br />
<li><strong>Best Poster:</strong> Freiburg bioware</li><br />
<li><strong>Best Presentation:</strong> ArtScienceBangalore</li><br />
<li><strong>Best New Standard:</strong> Heidelberg</li><br />
</td><br />
</tr><br />
</table><br />
<p style="font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> See the <a href="https://2009.igem.org/Results">Results page</a> for details <small>(Coming Soon)</small></p><br />
</div> <!-- close Jamboree results box --><br />
<br />
<br />
<br />
<!-- ***** Begin entry style portion of page ***** --><br />
<br />
<br />
<br />
<br />
<!-- *** ARCHIVE *** --><br />
<a href="https://2009.igem.org/Main_page_archive"><small>(News post archives)</small></a><br />
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<div class='right-heading'>About iGEM</div><br />
* [[About | What is iGEM?]]<br />
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* [[Previous iGEM Competitions | Previous iGEM competitions]]<br />
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<div class='right-heading'>iGEM Start to Finish</div><br />
* [[Calendar of Events | Calendar of events]]<br />
* [[Start_A_Team | Start a team]]<br />
* [[Requirements]]<br />
* [https://igem.org/IGEM_2009_Registration iGEM 2009 Registration]<br />
* [[Workshops| Spring workshops]]<br />
* [[Summer_events | Summer News & Events]]<br />
* [[Jamboree | The Jamboree]]<br />
** [[Jamboree/Attendance fee | Registration]]<br />
** [[Jamboree/Schedule | Schedule]]<br />
** [[Judging | Judging]]<br />
* [[Photo_Gallery | iGEM Photo Gallery]]<br />
* <span style="font-weight:bold; font-size:115%;">[[iGEM_Publicity | iGEM Publicity]]</span><br />
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* [[Security]]<br />
</div> <!-- close Start-to-finish --><br />
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<div class='right-heading'>Resources</div><br />
<!--* [[Mailing_Lists | Mailing lists]]--><br />
* [https://igem.org/Team_List?year=2009 2009 teams]<br />
* [https://2009.igem.org/Team_Wikis?year=2009 2009 team wikis]<br />
* [https://igem.org/Team_Parts?year=2009 2009 team parts]<br />
* [https://igem.org/Team_Judging?year=2009 2009 judging forms]<br />
* [[Partner_Offers | iGEM Partner Offers]]<br />
* [[Instructional Videos]]<br />
* [[Using the wiki]]<br />
* [http://partsregistry.org Registry of Standard Parts]<br />
* [[Main page archive | Main page archive]]<br />
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alt="iGEM forum button."<br />
title="Ask all your questions on the iGEM forum."><br><br />
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<img src="https://static.igem.org/mediawiki/2009/e/ec/Mathworks_Logo_R1_RGB_10per.jpg"<br />
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<img src="https://static.igem.org/mediawiki/2009/9/93/Mit_logo_h26px.jpg"<br />
style="padding: 0px 10px 0px 0px;"><br />
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<img src="https://static.igem.org/mediawiki/2009/e/e9/SynBERC_logo_h26px.png"<br />
style="padding: 0px 10px 0px 0px;"><br />
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<img src="https://static.igem.org/mediawiki/2009/6/6c/Nsf_logo_h26px.jpg"<br />
style="padding: 0px 10px 0px 0px;"><br />
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<img src="https://static.igem.org/mediawiki/2009/6/6f/BBF_h30px.png"<br />
style="padding: 0px 10px 0px 0px;"><br />
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--><br />
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<a href="https://2009.igem.org/Sponsors"><br />
<img src="https://static.igem.org/mediawiki/2009/d/df/Sponsor_footer_trim.png"<br />
style="padding-left:10px;"<br />
alt="iGEM 2009 Sponsors"<br />
title="iGEM 2009 Sponsors"><br />
</a><br />
<a href="https://2009.igem.org/Sponsorship"><br />
<img src="https://static.igem.org/mediawiki/2009/8/8d/Sponsor_now_h35px.png"<br />
style="padding-left:10px;"<br />
alt="Become an iGEM sponsor"<br />
title="Become an iGEM sponsor"><br />
</a><br />
</div><br />
</html></div>Meaganhttp://2009.igem.org/Main_PageMain Page2009-11-03T18:42:57Z<p>Meagan: </p>
<hr />
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<img src="https://static.igem.org/mediawiki/2009/0/00/Freezer_plates_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 15px;"<br />
alt="Main page image: freezer plates"<br />
title="Repository freezer plates."><br />
<img src="https://static.igem.org/mediawiki/2009/3/3d/Slovenia_mascot_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: Slovenia mascot."<br />
title="iGEM 2008 Slovenia mascot."><br />
<img src="https://static.igem.org/mediawiki/2009/5/57/Igem_banner_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: iGEM banner." <br />
title="iGEM banner."> <br />
<!-- <img src="https://static.igem.org/mediawiki/2009/3/37/Finalist_announce_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: 2008 finalist announcement"<br />
title="Announcing the finalists at the 2008 Jamboree."> --><br />
<!-- <img src="https://static.igem.org/mediawiki/2009/2/23/Dhl_boxes_2_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: DHL boxes"<br />
title="DHL boxes for the DNA distribution."> --><br />
<img src="https://static.igem.org/mediawiki/2009/3/38/Blackboard_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: Tagging blackboard!"<br />
title="Tagging the iGEM blackboard!"><br />
<img src="https://static.igem.org/mediawiki/2009/3/3a/Phage_mascot_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: Heidelberg phage mascot"<br />
title="iGEM 2008 Heidelberg phage mascot."><br />
<!-- <img src="https://static.igem.org/mediawiki/2009/9/9e/Presentation_room_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: 2008 Jamboree presentation room"<br />
title="2008 Jamboree presentation room."> --><br />
<img src="https://static.igem.org/mediawiki/2009/a/ab/Kit_plates_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: DNA distribution kit plates"<br />
title="DNA distribution kit plates."><br />
<br />
<div id="news_items" style="width: 700px; margin-left: 20px; float:left;"> <br />
<br />
<!-- *** QUICK LINKS BOX *** --><br />
<div id="jamboree_highlight_box" style="border:3px solid #fed801; width:700px; margin:0px 0px 30px 0px; padding:5px; background-color:#fbefb8;"><br />
<table style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; background-color:#fbefb8;"><br />
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<td style="width:375px;"><br />
<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> <a class="quick_link" href="https://2009.igem.org/Jamboree">iGEM 2009 Jamboree</a></h3><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center; font-weight:bold;">October 31 to November 2, 2009</p><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;">Massachusetts Institute of Technology</p><br />
<ul style="list-style:none;" ><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Quick links:</lh><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Project_Abstract/Team_Abstracts">Team abstracts</a></li><br />
<li><a class="quick_link" href="https://igem.org/Team_Wikis?year=2009">Team websites</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Schedule">Schedule</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Campus_map">Campus Map</a></li><br />
<li class="pending_results">iGEM 2009 Jamboree results</li><br />
</ul><br />
</td><br />
</td><br />
<td style="width:325px;"><br />
<a href="https://2009.igem.org/Image:2009_iGEM_from_Above_highres.jpg"><br />
<img src="https://static.igem.org/mediawiki/2009/9/95/2009_igem_from_above_300.jpg"<br />
style="margin-right:-110px;"><br />
</a><br />
</td><br />
</tr><br />
</table><br />
</div><br />
<br />
<!-- *** JAMBOREE RESULTS *** --><br />
<div id="jamboree_results_box" style="width:700px; margin:0px 0px 30px 0px;"><br />
<p style="font-size: 200%; font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#ed0100; text-align:center; margin-left:20px;">iGEM 2009 is officially over!</p><br />
<img src="https://static.igem.org/mediawiki/2009/2/2a/Jamboree2009_cambridge_award.jpg"<br />
style="margin-left:125px; border:2px solid #aaa"><br />
<br />
<!-- GRAND PRIZE, 1ST, 2ND --><br />
<ul style="list-style:none; font-size:110%; color:#666; font-family: Lucida Grande, Verdana, Arial, sans-serif; text-align:center;"><br />
<li><strong>Grand Prize, Winner of the BioBrick Trophy:</strong> Cambridge</li><br />
<li><strong>1st Runner Up:</strong> Heidelberg</li><br />
<li><strong>2nd Runner Up:</strong> Valencia</li><br />
</ul><br />
<br />
<!-- FINALISTS --><br />
<div id="finalist_list"><br />
<ul style="list-style: none;"><br />
<lh>Finalists:</lh><br />
<li>Cambridge</li><br />
<li>Freiburg bioware</li><br />
<li>Groningen</li><br />
<li>Heidelberg</li><br />
<li>Imperial College London</li><br />
<li>Valencia</li><br />
</ul><br />
</div> <!-- close finalists --><br />
<br />
<!-- TRACK AND SPECIAL AWARDS --><br />
<table style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666;"><br />
<tr><br />
<td style="width:350px;"><br />
<ul style="list-style:none;"><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Track Award Winners:</lh><br />
<li><strong>Best Food or Energy Project:</strong> UNIPV-Pavia</li><br />
<li><strong>Best Environment Project:</strong> Cambridge</li><br />
<li><strong>Best Health or Medicine Project:</strong> Stanford</li><br />
<li><strong>Best Manufacturing Project:</strong> Imperial College London</li><br />
<li><strong>Best New Application Area:</strong> Valencia</li><br />
<li><strong>Best Foundation Advance:</strong> Alberta</li><br />
<li><strong>Best Information Processing Project:</strong> TUDelft</li><br />
<li><strong>Best Software Tool:</strong> Berkeley Software & Illinois Software <small>(Tie)</small></li><br />
</ul><br />
</td><br />
<td style="width:350px;"><br />
<ul style="list-style:none;"><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Special Prizes Winners:</lh><br />
<li><strong>Best BioBrick Part, Natural:</strong> ULB-Brussels</li><br />
<li><strong>Best New BioBrick Part or Device, Engineered:</strong> EPF-Lausanne & Freiburg Bioware <small>(Tie)</small></li><br />
<li><strong>Best Human Practices Advance:</strong> Imperial College London & Paris <small>(Tie)</small></li><br />
<li><strong>Best Experimental Measurement:</strong> Valencia</li><br />
<li><strong>Best Model:</strong> BCCS-Bristol</li><br />
<li><strong>Best Wiki:</strong> Heidelberg</li><br />
<li><strong>Best Poster:</strong> Freiburg bioware</li><br />
<li><strong>Best Presentation:</strong> ArtScienceBangalore</li><br />
<li><strong>Best New Standard:</strong> Heidelberg</li><br />
</td><br />
</tr><br />
</table><br />
<p style="font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> See the <a href="https://2009.igem.org/Results">Results page</a> for details <small>(Coming Soon)</small></p><br />
</div> <!-- close Jamboree results box --><br />
<br />
<br />
<br />
<!-- ***** Begin entry style portion of page ***** --><br />
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<br />
<!-- *** ARCHIVE *** --><br />
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* [[About | What is iGEM?]]<br />
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<div class='right-heading'>iGEM Start to Finish</div><br />
* [[Calendar of Events | Calendar of events]]<br />
* [[Start_A_Team | Start a team]]<br />
* [[Requirements]]<br />
* [https://igem.org/IGEM_2009_Registration iGEM 2009 Registration]<br />
* [[Workshops| Spring workshops]]<br />
* [[Summer_events | Summer News & Events]]<br />
* [[Jamboree | The Jamboree]]<br />
** [[Jamboree/Attendance fee | Registration]]<br />
** [[Jamboree/Schedule | Schedule]]<br />
** [[Judging | Judging]]<br />
* [[Photo_Gallery | iGEM Photo Gallery]]<br />
* <span style="font-weight:bold; font-size:115%;">[[iGEM_Publicity | iGEM Publicity]]</span><br />
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</div> <!-- close Start-to-finish --><br />
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<!--* [[Mailing_Lists | Mailing lists]]--><br />
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<a href="https://2009.igem.org/Sponsors"><br />
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title="iGEM 2009 Sponsors"><br />
</a><br />
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<img src="https://static.igem.org/mediawiki/2009/8/8d/Sponsor_now_h35px.png"<br />
style="padding-left:10px;"<br />
alt="Become an iGEM sponsor"<br />
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</a><br />
</div><br />
</html></div>Meaganhttp://2009.igem.org/File:2009_igem_from_above_300.jpgFile:2009 igem from above 300.jpg2009-11-03T18:39:14Z<p>Meagan: </p>
<hr />
<div></div>Meaganhttp://2009.igem.org/Photo_GalleryPhoto Gallery2009-11-03T18:38:59Z<p>Meagan: New page: <div style='float:left;width:400px;margin-right:40px;text-align:center;'> [http://www.flickr.com/photos/igemhq/ <font size=4><font color=dodgerblue>'''flick</font><font color=crimson>r&tra...</p>
<hr />
<div><div style='float:left;width:400px;margin-right:40px;text-align:center;'><br />
[http://www.flickr.com/photos/igemhq/ <font size=4><font color=dodgerblue>'''flick</font><font color=crimson>r&trade;</font> <font color=black>photos'''</font></font>]<br><br />
<small>(Photos are licensed under [http://creativecommons.org/about/licenses Creative Commons - Attribution] license. Please attribute iGEM 2009 Jamboree photos to '''David Appleyard''' and '''iGEM'''.)</small><br />
[http://www.flickr.com/photos/igemhq/ https://static.igem.org/mediawiki/2008/6/6e/Igem_08_blackboard_xsmall.jpg]<br />
<br />
If you upload pictures that you took yourself, use the <font size=3>'''igem'''</font> and <font size=3>'''2009'''</font> tags so that everyone else can find them too.<br />
<div><br />
Post the links to your pictures in the list to the right.<br />
</div><br />
</div><br />
<br />
<br />
<font size=4>'''Pictures taken by the iGEM Community:'''</font><br />
* iGEM team Example, [http://flickr.com/photos/igemhq iGEM HQ Flickr page]</div>Meaganhttp://2009.igem.org/Main_PageMain Page2009-11-03T18:35:09Z<p>Meagan: </p>
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<img src="https://static.igem.org/mediawiki/2009/0/00/Freezer_plates_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 15px;"<br />
alt="Main page image: freezer plates"<br />
title="Repository freezer plates."><br />
<img src="https://static.igem.org/mediawiki/2009/3/3d/Slovenia_mascot_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: Slovenia mascot."<br />
title="iGEM 2008 Slovenia mascot."><br />
<img src="https://static.igem.org/mediawiki/2009/5/57/Igem_banner_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: iGEM banner." <br />
title="iGEM banner."> <br />
<!-- <img src="https://static.igem.org/mediawiki/2009/3/37/Finalist_announce_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: 2008 finalist announcement"<br />
title="Announcing the finalists at the 2008 Jamboree."> --><br />
<!-- <img src="https://static.igem.org/mediawiki/2009/2/23/Dhl_boxes_2_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: DHL boxes"<br />
title="DHL boxes for the DNA distribution."> --><br />
<img src="https://static.igem.org/mediawiki/2009/3/38/Blackboard_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: Tagging blackboard!"<br />
title="Tagging the iGEM blackboard!"><br />
<img src="https://static.igem.org/mediawiki/2009/3/3a/Phage_mascot_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: Heidelberg phage mascot"<br />
title="iGEM 2008 Heidelberg phage mascot."><br />
<!-- <img src="https://static.igem.org/mediawiki/2009/9/9e/Presentation_room_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: 2008 Jamboree presentation room"<br />
title="2008 Jamboree presentation room."> --><br />
<img src="https://static.igem.org/mediawiki/2009/a/ab/Kit_plates_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: DNA distribution kit plates"<br />
title="DNA distribution kit plates."><br />
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<div id="news_items" style="width: 700px; margin-left: 20px; float:left;"> <br />
<br />
<!-- *** QUICK LINKS BOX *** --><br />
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<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> <a class="quick_link" href="https://2009.igem.org/Jamboree">iGEM 2009 Jamboree</a></h3><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center; font-weight:bold;">October 31 to November 2, 2009</p><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;">Massachusetts Institute of Technology</p><br />
<ul style="list-style:none;" ><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Quick links:</lh><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Project_Abstract/Team_Abstracts">Team abstracts</a></li><br />
<li><a class="quick_link" href="https://igem.org/Team_Wikis?year=2009">Team websites</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Schedule">Schedule</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Campus_map">Campus Map</a></li><br />
<li class="pending_results">iGEM 2009 Jamboree results</li><br />
</ul><br />
</td><br />
</td><br />
<td style="width:325px;"><br />
<a href="https://2009.igem.org/Jamboree"><br />
<img src="https://static.igem.org/mediawiki/2009/4/4c/Igem_from_above_300.jpg"<br />
style="margin-right:-110px;"><br />
</a><br />
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<br />
<!-- *** JAMBOREE RESULTS *** --><br />
<div id="jamboree_results_box" style="width:700px; margin:0px 0px 30px 0px;"><br />
<p style="font-size: 200%; font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#ed0100; text-align:center; margin-left:20px;">iGEM 2009 is officially over!</p><br />
<img src="https://static.igem.org/mediawiki/2009/2/2a/Jamboree2009_cambridge_award.jpg"<br />
style="margin-left:125px; border:2px solid #aaa"><br />
<br />
<!-- GRAND PRIZE, 1ST, 2ND --><br />
<ul style="list-style:none; font-size:110%; color:#666; font-family: Lucida Grande, Verdana, Arial, sans-serif; text-align:center;"><br />
<li><strong>Grand Prize, Winner of the BioBrick Trophy:</strong> Cambridge</li><br />
<li><strong>1st Runner Up:</strong> Heidelberg</li><br />
<li><strong>2nd Runner Up:</strong> Valencia</li><br />
</ul><br />
<br />
<!-- FINALISTS --><br />
<div id="finalist_list"><br />
<ul style="list-style: none;"><br />
<lh>Finalists:</lh><br />
<li>Cambridge</li><br />
<li>Freiburg bioware</li><br />
<li>Groningen</li><br />
<li>Heidelberg</li><br />
<li>Imperial College London</li><br />
<li>Valencia</li><br />
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<!-- TRACK AND SPECIAL AWARDS --><br />
<table style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666;"><br />
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<td style="width:350px;"><br />
<ul style="list-style:none;"><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Track Award Winners:</lh><br />
<li><strong>Best Food or Energy Project:</strong> UNIPV-Pavia</li><br />
<li><strong>Best Environment Project:</strong> Cambridge</li><br />
<li><strong>Best Health or Medicine Project:</strong> Stanford</li><br />
<li><strong>Best Manufacturing Project:</strong> Imperial College London</li><br />
<li><strong>Best New Application Area:</strong> Valencia</li><br />
<li><strong>Best Foundation Advance:</strong> Alberta</li><br />
<li><strong>Best Information Processing Project:</strong> TUDelft</li><br />
<li><strong>Best Software Tool:</strong> Berkeley Software & Illinois Software <small>(Tie)</small></li><br />
</ul><br />
</td><br />
<td style="width:350px;"><br />
<ul style="list-style:none;"><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Special Prizes Winners:</lh><br />
<li><strong>Best BioBrick Part, Natural:</strong> ULB-Brussels</li><br />
<li><strong>Best New BioBrick Part or Device, Engineered:</strong> EPF-Lausanne & Freiburg Bioware <small>(Tie)</small></li><br />
<li><strong>Best Human Practices Advance:</strong> Imperial College London & Paris <small>(Tie)</small></li><br />
<li><strong>Best Experimental Measurement:</strong> Valencia</li><br />
<li><strong>Best Model:</strong> BCCS-Bristol</li><br />
<li><strong>Best Wiki:</strong> Heidelberg</li><br />
<li><strong>Best Poster:</strong> Freiburg bioware</li><br />
<li><strong>Best Presentation:</strong> ArtScienceBangalore</li><br />
<li><strong>Best New Standard:</strong> Heidelberg</li><br />
</td><br />
</tr><br />
</table><br />
<p style="font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> See the <a href="https://2009.igem.org/Results">Results page</a> for details <small>(Coming Soon)</small></p><br />
</div> <!-- close Jamboree results box --><br />
<br />
<br />
<br />
<!-- ***** Begin entry style portion of page ***** --><br />
<br />
<br />
<br />
<br />
<!-- *** ARCHIVE *** --><br />
<a href="https://2009.igem.org/Main_page_archive"><small>(News post archives)</small></a><br />
<br />
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<div class='right-box' style='border-right:1px solid #390; border-top:2px solid #390; border-bottom:2px solid #390;background-color:#ceeac1;'><br />
<div class='right-heading'>About iGEM</div><br />
* [[About | What is iGEM?]]<br />
<!--* [[iGEM_Publicity | News about iGEM]]--><br />
* [[Previous iGEM Competitions | Previous iGEM competitions]]<br />
* [[Contact | iGEM Headquarters]]<br />
* [[FAQs | Frequently Asked Questions]]<br />
* [[Press_Kit | iGEM Press Kit]]<br />
* [http://mail.igem.org/mailman/listinfo/interest Join the iGEM Mailing List]<br />
* [[Sponsorship | Sponsor iGEM]]<br />
</div> <!-- close About --><br />
<br />
<div class='right-box' style='border-right:1px solid #309; border-top:2px solid #309; border-bottom:2px solid #309;background-color:#ddd0f5;'><br />
<div class='right-heading'>iGEM Start to Finish</div><br />
* [[Calendar of Events | Calendar of events]]<br />
* [[Start_A_Team | Start a team]]<br />
* [[Requirements]]<br />
* [https://igem.org/IGEM_2009_Registration iGEM 2009 Registration]<br />
* [[Workshops| Spring workshops]]<br />
* [[Summer_events | Summer News & Events]]<br />
* [[Jamboree | The Jamboree]]<br />
** [[Jamboree/Attendance fee | Registration]]<br />
** [[Jamboree/Schedule | Schedule]]<br />
** [[Judging | Judging]]<br />
* [[Photo_Gallery | iGEM Photo Gallery]]<br />
* <span style="font-weight:bold; font-size:115%;">[[iGEM_Publicity | iGEM Publicity]]</span><br />
* [[iGEM_Publications | iGEM Publications]]<br />
* [[Safety | Safety]]<br />
* [[Security]]<br />
</div> <!-- close Start-to-finish --><br />
<br />
<div class='right-box' style='border-right:1px solid #ff3366; border-top:2px solid #ff3366; border-bottom:2px solid #ff3366;background-color:#f4c8d3;'><br />
<div class='right-heading'>Resources</div><br />
<!--* [[Mailing_Lists | Mailing lists]]--><br />
* [https://igem.org/Team_List?year=2009 2009 teams]<br />
* [https://2009.igem.org/Team_Wikis?year=2009 2009 team wikis]<br />
* [https://igem.org/Team_Parts?year=2009 2009 team parts]<br />
* [https://igem.org/Team_Judging?year=2009 2009 judging forms]<br />
* [[Partner_Offers | iGEM Partner Offers]]<br />
* [[Instructional Videos]]<br />
* [[Using the wiki]]<br />
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</html></div>Meaganhttp://2009.igem.org/Main_PageMain Page2009-11-03T18:34:31Z<p>Meagan: </p>
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title="Repository freezer plates."><br />
<img src="https://static.igem.org/mediawiki/2009/3/3d/Slovenia_mascot_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
alt="Main page image: Slovenia mascot."<br />
title="iGEM 2008 Slovenia mascot."><br />
<img src="https://static.igem.org/mediawiki/2009/5/57/Igem_banner_xsmall.jpg"<br />
style="width:15%; height:15%; border:2px solid #aaa; margin:-10px 5px 10px 0px;"<br />
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title="iGEM banner."> <br />
<!-- <img src="https://static.igem.org/mediawiki/2009/3/37/Finalist_announce_xsmall.jpg"<br />
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<br />
<!-- *** QUICK LINKS BOX *** --><br />
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<h3 style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> <a class="quick_link" href="https://2009.igem.org/Jamboree">iGEM 2009 Jamboree</a></h3><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center; font-weight:bold;">October 31 to November 2, 2009</p><br />
<p style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;">Massachusetts Institute of Technology</p><br />
<ul style="list-style:none;" ><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Quick links:</lh><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Project_Abstract/Team_Abstracts">Team abstracts</a></li><br />
<li><a class="quick_link" href="https://igem.org/Team_Wikis?year=2009">Team websites</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Schedule">Schedule</a></li><br />
<li><a class="quick_link" href="https://2009.igem.org/Jamboree/Campus_map">Campus Map</a></li><br />
<li class="pending_results">iGEM 2009 Jamboree results</li><br />
</ul><br />
</td><br />
</td><br />
<td style="width:325px;"><br />
<a href="https://2009.igem.org/Jamboree"><br />
<img src="https://static.igem.org/mediawiki/2009/4/4c/Igem_from_above_300.jpg"<br />
style="margin-right:-110px;"><br />
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<br />
<!-- *** JAMBOREE RESULTS *** --><br />
<div id="jamboree_results_box" style="width:700px; margin:0px 0px 30px 0px;"><br />
<p style="font-size: 200%; font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#ed0100; text-align:center; margin-left:20px;">iGEM 2009 is officially over!</p><br />
<img src="https://static.igem.org/mediawiki/2009/2/2a/Jamboree2009_cambridge_award.jpg"<br />
style="margin-left:125px; border:2px solid #aaa"><br />
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<!-- GRAND PRIZE, 1ST, 2ND --><br />
<ul style="list-style:none; font-size:110%; color:#666; font-family: Lucida Grande, Verdana, Arial, sans-serif; text-align:center;"><br />
<li><strong>Grand Prize, Winner of the BioBrick Trophy:</strong> Cambridge</li><br />
<li><strong>1st Runner Up:</strong> Heidelberg</li><br />
<li><strong>2nd Runner Up:</strong> Valencia</li><br />
</ul><br />
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<!-- FINALISTS --><br />
<div id="finalist_list"><br />
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<lh>Finalists:</lh><br />
<li>Cambridge</li><br />
<li>Freiburg bioware</li><br />
<li>Groningen</li><br />
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<table style="font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666;"><br />
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<ul style="list-style:none;"><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Track Award Winners:</lh><br />
<li><strong>Best Food or Energy Project:</strong> UNIPV-Pavia</li><br />
<li><strong>Best Environment Project:</strong> Cambridge</li><br />
<li><strong>Best Health or Medicine Project:</strong> Stanford</li><br />
<li><strong>Best Manufacturing Project:</strong> Imperial College London</li><br />
<li><strong>Best New Application Area:</strong> Valencia</li><br />
<li><strong>Best Foundation Advance:</strong> Alberta</li><br />
<li><strong>Best Information Processing Project:</strong> TUDelft</li><br />
<li><strong>Best Software Tool:</strong> Berkeley Software & Illinois Software <small>(Tie)</small></li><br />
</ul><br />
</td><br />
<td style="width:350px;"><br />
<ul style="list-style:none;"><br />
<lh style="font-size:110%; font-weight:bold; color:#576f91;">Special Prizes Winners:</lh><br />
<li><strong>Best BioBrick Part, Natural:</strong> ULB-Brussels</li><br />
<li><strong>Best New BioBrick Part or Device, Engineered:</strong> EPF-Lausanne & Freiburg Bioware <small>(Tie)</small></li><br />
<li><strong>Best Human Practices Advance:</strong> Imperial College London & Paris <small>(Tie)</small></li><br />
<li><strong>Best Experimental Measurement:</strong> Valencia</li><br />
<li><strong>Best Model:</strong> BCCS-Bristol</li><br />
<li><strong>Best Wiki:</strong> Heidelberg</li><br />
<li><strong>Best Poster:</strong> Freiburg bioware</li><br />
<li><strong>Best Presentation:</strong> ArtScienceBangalore</li><br />
<li><strong>Best New Standard:</strong> Heidelberg</li><br />
</td><br />
</tr><br />
</table><br />
<p style="font-weight:bold; font-family: Lucida Grande, Verdana, Arial, sans-serif; color:#666; text-align:center;"> See the <a href="https://2009.igem.org/Results">Results page</a> for details <small>(Coming Soon)</small></p><br />
</div> <!-- close Jamboree results box --><br />
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</html></div>Meagan