User contributions
From 2009.igem.org
(Latest | Earliest) View (newer 250 | older 250) (20 | 50 | 100 | 250 | 500)
- 10:10, 20 October 2009 (diff | hist) Team:HKUST/Lab Notebook (top)
- 08:51, 20 October 2009 (diff | hist) Team:TeamMembers
- 08:51, 20 October 2009 (diff | hist) Team:TeamMembers
- 16:14, 19 October 2009 (diff | hist) Team:HKUST/Lab Notebook
- 16:12, 19 October 2009 (diff | hist) Team:HKUST/Lab Notebook
- 16:03, 19 October 2009 (diff | hist) Team:HKUST/Protocols/GFP testing
- 16:02, 19 October 2009 (diff | hist) Team:HKUST/Protocols/Western blotting
- 16:01, 19 October 2009 (diff | hist) Team:HKUST/Protocols/Western blotting
- 16:00, 19 October 2009 (diff | hist) Team:HKUST/Protocols/Yeast genomic DNA extraction
- 15:58, 19 October 2009 (diff | hist) Team:HKUST/Protocols/Cell lysis
- 15:56, 19 October 2009 (diff | hist) Team:HKUST/Protocols/Transformation to yeast
- 15:53, 19 October 2009 (diff | hist) Team:HKUST/Protocols/Transformation to Ecoli
- 15:51, 19 October 2009 (diff | hist) Team:HKUST/Protocols/Ligation
- 15:49, 19 October 2009 (diff | hist) Team:HKUST/Protocols/gel extraction
- 15:49, 19 October 2009 (diff | hist) Team:HKUST/Protocols/plasmid extraction
- 15:48, 19 October 2009 (diff | hist) Team:HKUST/Protocols/Enzyme digestion
- 15:48, 19 October 2009 (diff | hist) Team:HKUST/Protocols/PCR cleanup
- 15:47, 19 October 2009 (diff | hist) Team:HKUST/Protocols/PCR
- 15:47, 19 October 2009 (diff | hist) Team:HKUST/Protocols/Ethanol precipitation
- 15:45, 19 October 2009 (diff | hist) Team:HKUST/Protocols/gel extraction
- 15:43, 19 October 2009 (diff | hist) Team:HKUST/Protocols/gel cut
- 15:40, 19 October 2009 (diff | hist) Team:HKUST/Protocols/Enzyme digestion
- 15:39, 19 October 2009 (diff | hist) Team:HKUST/Protocols/plasmid extraction
- 15:36, 19 October 2009 (diff | hist) Team:HKUST/Protocols/Enzyme digestion
- 15:34, 19 October 2009 (diff | hist) Team:HKUST/Protocols/PCR
- 15:32, 19 October 2009 (diff | hist) Team:HKUST/Protocols/PCR
- 15:30, 19 October 2009 (diff | hist) Team:HKUST/Protocols/PCR cleanup
- 15:28, 19 October 2009 (diff | hist) Team:HKUST/Protocols/PCR
- 15:22, 19 October 2009 (diff | hist) Team:HKUST/Protocols/Agarose gel
- 15:22, 19 October 2009 (diff | hist) Team:HKUST/Protocols
- 06:01, 18 October 2009 (diff | hist) Team:HKUST/Lab Notebook
- 13:41, 17 October 2009 (diff | hist) Team:HKUST/Group4
- 13:40, 17 October 2009 (diff | hist) Team:HKUST/Group4
- 13:38, 17 October 2009 (diff | hist) Team:HKUST/Group4
- 13:34, 17 October 2009 (diff | hist) Team:HKUST/Back4
- 13:33, 17 October 2009 (diff | hist) Team:HKUST/Ref4
- 13:24, 17 October 2009 (diff | hist) Team:HKUST/Ref4
- 13:23, 17 October 2009 (diff | hist) Team:HKUST/Back4
- 13:20, 17 October 2009 (diff | hist) Team:HKUST/Future4
- 13:19, 17 October 2009 (diff | hist) N Team:HKUST/Group4 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 13:16, 17 October 2009 (diff | hist) Team:HKUST/Back4
- 13:16, 17 October 2009 (diff | hist) Team:HKUST/Back4
- 13:15, 17 October 2009 (diff | hist) N Team:HKUST/Back4 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 13:13, 17 October 2009 (diff | hist) N Team:HKUST/View4 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...) (top)
- 07:15, 11 October 2009 (diff | hist) Team:HKUST/Group1
- 07:13, 11 October 2009 (diff | hist) Team:HKUST/Group1
- 07:12, 11 October 2009 (diff | hist) Team:HKUST/Back1
- 07:08, 11 October 2009 (diff | hist) Team:HKUST/Part3
- 07:06, 11 October 2009 (diff | hist) Team:HKUST/Back3
- 07:05, 11 October 2009 (diff | hist) Team:HKUST/Part3
- 07:05, 11 October 2009 (diff | hist) Team:HKUST/Group3
- 06:59, 11 October 2009 (diff | hist) Team:HKUST/Group3
- 06:58, 11 October 2009 (diff | hist) Team:HKUST/Group3
- 06:53, 11 October 2009 (diff | hist) Team:HKUST/Group3
- 06:52, 11 October 2009 (diff | hist) Team:HKUST/Part3
- 06:48, 11 October 2009 (diff | hist) Team:HKUST/Group3
- 06:38, 11 October 2009 (diff | hist) Team:HKUST/Group3
- 06:30, 11 October 2009 (diff | hist) Team:HKUST/Back3
- 06:29, 11 October 2009 (diff | hist) Team:HKUST/Back3
- 02:36, 10 October 2009 (diff | hist) Team:HKUST/Lab Notebook
- 02:36, 10 October 2009 (diff | hist) Team:HKUST/View3 (top)
- 02:35, 10 October 2009 (diff | hist) Team:HKUST/Ref1
- 02:35, 10 October 2009 (diff | hist) Team:HKUST/Future1
- 02:35, 10 October 2009 (diff | hist) Team:HKUST/Result1
- 02:35, 10 October 2009 (diff | hist) Team:HKUST/Part1
- 02:34, 10 October 2009 (diff | hist) Team:HKUST/Group1
- 02:34, 10 October 2009 (diff | hist) Team:HKUST/Back1
- 02:34, 10 October 2009 (diff | hist) Team:HKUST/View1
- 02:33, 10 October 2009 (diff | hist) Team:HKUST/Project
- 02:32, 10 October 2009 (diff | hist) Team:HKUST/Project
- 02:32, 10 October 2009 (diff | hist) Team:HKUST
- 02:30, 10 October 2009 (diff | hist) N Team:HKUST/Part3 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 02:26, 10 October 2009 (diff | hist) N Team:HKUST/Result3 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 02:25, 10 October 2009 (diff | hist) Team:HKUST/Group3
- 02:21, 10 October 2009 (diff | hist) N Team:HKUST/Ref3 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 02:21, 10 October 2009 (diff | hist) Team:HKUST/Ref4 (Removing all content from page)
- 02:20, 10 October 2009 (diff | hist) N Team:HKUST/Ref4 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 02:19, 10 October 2009 (diff | hist) N Team:HKUST/Future3 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 02:17, 10 October 2009 (diff | hist) Team:HKUST/Back3
- 02:13, 10 October 2009 (diff | hist) Team:HKUST/Back3
- 02:12, 10 October 2009 (diff | hist) Team:HKUST/View1
- 02:11, 10 October 2009 (diff | hist) Team:HKUST/View1
- 02:11, 10 October 2009 (diff | hist) Team:HKUST/View3
- 02:09, 10 October 2009 (diff | hist) N Team:HKUST/Back3 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 02:06, 10 October 2009 (diff | hist) N Team:HKUST/View3 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 15:47, 9 October 2009 (diff | hist) Team:HKUST
- 15:47, 9 October 2009 (diff | hist) Team:HKUST/Part1
- 11:49, 8 October 2009 (diff | hist) Team:HKUST
- 11:48, 8 October 2009 (diff | hist) N Team:HKUST/Future1 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 11:47, 8 October 2009 (diff | hist) Team:HKUST/Ref1
- 11:46, 8 October 2009 (diff | hist) Team:HKUST/Result1
- 11:46, 8 October 2009 (diff | hist) Team:HKUST/Part1
- 11:45, 8 October 2009 (diff | hist) Team:HKUST/Group1
- 11:45, 8 October 2009 (diff | hist) Team:HKUST/Back1
- 11:45, 8 October 2009 (diff | hist) Team:HKUST/View1
- 11:44, 8 October 2009 (diff | hist) Team:HKUST/View1
- 11:43, 8 October 2009 (diff | hist) Team:HKUST/Part1
- 11:42, 8 October 2009 (diff | hist) Team:HKUST/Part1
- 11:42, 8 October 2009 (diff | hist) Team:HKUST/Part1
- 11:40, 8 October 2009 (diff | hist) Team:HKUST/Part1
- 11:36, 8 October 2009 (diff | hist) N Team:HKUST/Part1 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 11:34, 8 October 2009 (diff | hist) Team:HKUST/Group1
- 11:33, 8 October 2009 (diff | hist) Team:HKUST/Group1
- 11:21, 8 October 2009 (diff | hist) N Team:HKUST/Future4 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 11:10, 8 October 2009 (diff | hist) N Team:HKUST/Result1 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 11:06, 8 October 2009 (diff | hist) Team:HKUST/Ref1
- 11:06, 8 October 2009 (diff | hist) Team:HKUST/Ref1
- 11:05, 8 October 2009 (diff | hist) N Team:HKUST/Ref1 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 10:59, 8 October 2009 (diff | hist) Team:HKUST/Background (Removing all content from page) (top)
- 10:57, 8 October 2009 (diff | hist) Team:HKUST/Back1
- 10:48, 8 October 2009 (diff | hist) Team:HKUST/Back1
- 10:38, 8 October 2009 (diff | hist) N Team:HKUST/Back1 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 10:37, 8 October 2009 (diff | hist) N Team:HKUST/View1 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 10:36, 8 October 2009 (diff | hist) Team:HKUST
- 14:09, 7 October 2009 (diff | hist) Team:HKUST
- 11:35, 6 October 2009 (diff | hist) Team:HKUST/Group2 (top)
- 11:09, 6 October 2009 (diff | hist) Team:HKUST/Group2
- 11:06, 6 October 2009 (diff | hist) N Team:HKUST/Group2 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 08:46, 4 October 2009 (diff | hist) Team:HKUST/Group3
- 08:39, 4 October 2009 (diff | hist) N Team:HKUST/Group3 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 08:34, 4 October 2009 (diff | hist) Team:HKUST/Group1
- 08:34, 4 October 2009 (diff | hist) Team:HKUST/Group1
- 08:29, 4 October 2009 (diff | hist) Team:HKUST/Group1
- 08:26, 4 October 2009 (diff | hist) Team:HKUST/Group1
- 08:25, 4 October 2009 (diff | hist) Team:HKUST/Group1
- 08:24, 4 October 2009 (diff | hist) Team:HKUST/Group1
- 08:20, 4 October 2009 (diff | hist) Team:HKUST/Group1
- 08:13, 4 October 2009 (diff | hist) N Team:HKUST/Group1 (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 07:54, 4 October 2009 (diff | hist) Team:HKUST/Experiment (top)
- 10:59, 1 October 2009 (diff | hist) Team:HKUST/Result (top)
- 10:59, 1 October 2009 (diff | hist) Team:HKUST/Resourses
- 10:58, 1 October 2009 (diff | hist) Team:HKUST/Result
- 10:58, 1 October 2009 (diff | hist) Team:HKUST/Lab Notebook
- 10:57, 1 October 2009 (diff | hist) Team:HKUST
- 10:56, 1 October 2009 (diff | hist) Team:HKUST/Project
- 10:54, 1 October 2009 (diff | hist) Team:HKUST/Resourses
- 10:54, 1 October 2009 (diff | hist) Team:HKUST/Protocols
- 10:53, 1 October 2009 (diff | hist) Team:HKUST/Team
- 10:52, 1 October 2009 (diff | hist) Team:HKUST/Protocols
- 10:50, 1 October 2009 (diff | hist) N Team:HKUST/Protocols/GFP testing (New page: ===Fluorescence microscopy visualization=== *1. Grow yeast transformed cells containing appropriate plasmids in 5 ml SCM minus His & glucose at 30ºC overnight.. *2. Add galactose (to a co...)
- 10:48, 1 October 2009 (diff | hist) Team:HKUST/Protocols/Western blotting (→Western Blotting)
- 10:47, 1 October 2009 (diff | hist) N Team:HKUST/Protocols/Western blotting (New page: ===Western Blotting=== 1. Yeast transformed cells containing appropriate plasmids were grown in SCM with appropriate amino acids deleted and harvested at a cell density corresponding to an...)
- 02:11, 29 September 2009 (diff | hist) Team:HKUST/Background
- 02:09, 29 September 2009 (diff | hist) Team:HKUST/Background
- 02:08, 29 September 2009 (diff | hist) Team:HKUST/Background
- 02:07, 29 September 2009 (diff | hist) Team:HKUST/Future (top)
- 02:07, 29 September 2009 (diff | hist) Team:HKUST/Experiment
- 02:06, 29 September 2009 (diff | hist) N Team:HKUST/Background (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 02:06, 29 September 2009 (diff | hist) Team:HKUST/Experiment
- 12:45, 28 September 2009 (diff | hist) N Team:Acknowledgement (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 12:45, 28 September 2009 (diff | hist) N Team:Consolidation (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 12:45, 28 September 2009 (diff | hist) N Team:Gallery (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 12:45, 28 September 2009 (diff | hist) Team:HKUST/Future
- 12:44, 28 September 2009 (diff | hist) Team:HKUST/Resourses
- 12:44, 28 September 2009 (diff | hist) Team:HKUST/Protocols
- 12:44, 28 September 2009 (diff | hist) Team:HKUST/Lab Notebook
- 12:43, 28 September 2009 (diff | hist) Team:HKUST/Experiment
- 12:43, 28 September 2009 (diff | hist) Team:HKUST/Project
- 12:43, 28 September 2009 (diff | hist) Team:HKUST/Project
- 12:42, 28 September 2009 (diff | hist) Team:HKUST
- 12:41, 28 September 2009 (diff | hist) Team:HKUST
- 12:37, 28 September 2009 (diff | hist) N Team:HKUST/Result (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 12:37, 28 September 2009 (diff | hist) N Team:HKUST/Future (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 12:36, 28 September 2009 (diff | hist) N Team:HKUST/Experiment (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 12:35, 28 September 2009 (diff | hist) Team:HKUST/Lab Notebook
- 12:31, 28 September 2009 (diff | hist) Team:HKUST
- 12:30, 28 September 2009 (diff | hist) Team:HKUST
- 12:29, 28 September 2009 (diff | hist) Team:HKUST
- 12:27, 28 September 2009 (diff | hist) Team:HKUST
- 12:17, 28 September 2009 (diff | hist) Team:HKUST/Project
- 12:16, 28 September 2009 (diff | hist) Team:HKUST/Members (top)
- 12:16, 28 September 2009 (diff | hist) Team:HKUST/Resourses
- 12:13, 28 September 2009 (diff | hist) Team:HKUST/Resourses
- 12:13, 28 September 2009 (diff | hist) Team:HKUST
- 12:12, 28 September 2009 (diff | hist) Team:HKUST
- 12:11, 28 September 2009 (diff | hist) Team:HKUST
- 12:11, 28 September 2009 (diff | hist) Team:HKUST
- 12:10, 28 September 2009 (diff | hist) Team:HKUST
- 13:51, 21 September 2009 (diff | hist) Team:HKUST/Resourses
- 13:49, 21 September 2009 (diff | hist) N Team:HKUST/Resourses (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 13:34, 21 September 2009 (diff | hist) Team:HKUST/Protocols
- 13:33, 21 September 2009 (diff | hist) Team:HKUST/Project
- 13:32, 21 September 2009 (diff | hist) Team:HKUST/Members
- 13:31, 21 September 2009 (diff | hist) Team:HKUST
- 13:30, 21 September 2009 (diff | hist) Team:HKUST/Project
- 13:23, 21 September 2009 (diff | hist) Team:HKUST/Protocols
- 13:20, 21 September 2009 (diff | hist) Team:HKUST/Protocols
- 11:18, 17 September 2009 (diff | hist) Team:HKUST
- 10:42, 17 September 2009 (diff | hist) Team:HKUST
- 10:39, 17 September 2009 (diff | hist) Team:HKUST
- 08:07, 12 September 2009 (diff | hist) Team:HKUST
- 08:07, 12 September 2009 (diff | hist) Template:HKUSTHOME (Removing all content from page) (top)
- 08:00, 12 September 2009 (diff | hist) N Template:HKUSTHOME (New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
- 08:00, 12 September 2009 (diff | hist) Team:HKUST (Replacing page with '{{Template:HKUSTHOME}}')
- 07:48, 12 September 2009 (diff | hist) Team:HKUST
- 07:39, 12 September 2009 (diff | hist) Team:HKUST
- 07:33, 12 September 2009 (diff | hist) Team:HKUST
- 07:28, 12 September 2009 (diff | hist) Team:HKUST
- 07:20, 12 September 2009 (diff | hist) Team:HKUST
- 07:19, 12 September 2009 (diff | hist) Team:HKUST
- 16:08, 26 August 2009 (diff | hist) Template:HKUST iGEM 09 (top)
- 16:08, 26 August 2009 (diff | hist) Template:HKUST iGEM 09
- 16:05, 26 August 2009 (diff | hist) Template:HKUST iGEM 09
- 15:50, 26 August 2009 (diff | hist) Team:HKUST/Protocols/Agarose gel
- 15:50, 26 August 2009 (diff | hist) Team:HKUST/Protocols/Agarose gel
- 15:41, 26 August 2009 (diff | hist) Template:HKUST iGEM 09
- 15:36, 26 August 2009 (diff | hist) N File:HKUST footer.jpg (top)
- 14:15, 26 August 2009 (diff | hist) Team:HKUST/Lab Notebook
- 14:15, 26 August 2009 (diff | hist) Team:HKUST/Lab Notebook
- 14:15, 26 August 2009 (diff | hist) Team:HKUST/Lab Notebook
- 14:14, 26 August 2009 (diff | hist) Team:HKUST/Lab Notebook
- 14:06, 26 August 2009 (diff | hist) Team:HKUST/Protocols
- 14:02, 26 August 2009 (diff | hist) N Team:HKUST/Protocols/Yeast genomic DNA extraction (New page: 13.Yeast genomic DNA extraction Purpose: To extract yeast genomic DNA from yeast strain (strain YPH501, in our case). Materials: yeast strain, lysis buffer, phenol, chloroform, 3M Na...)
- 14:02, 26 August 2009 (diff | hist) N Team:HKUST/Protocols/Cell lysis (New page: 12.Cell lysis Purpose: to lyse E.coli cell to get linearized ligated plasmid. Materials: cultured transformed E.coli colony, phenol, chloroform, ddH2O Procedure: a.Add 40μL ...)
- 14:01, 26 August 2009 (diff | hist) N Team:HKUST/Protocols/Transformation to yeast (New page: 11.Transformation to E.coli Purpose: To transform desired plasmid DNA in to E.coli in order to amplify it. Materials: E.coli competent cell, desired plasmid DNA, LB media, agar plate...)
- 14:01, 26 August 2009 (diff | hist) N Team:HKUST/Protocols/Transformation to Ecoli (New page: 10. Transformation to yeast Purpose: To transform desired constructed plamid DNA to yeast to test its effect. Materials: LiOAc solution, PEG4000 solution, DMSO (dimethyl sulfoxide), ...)
- 14:01, 26 August 2009 (diff | hist) N Team:HKUST/Protocols/Ligation (New page: 9. Ligation Purpose: To ligate desired insert DNA fragment into vector with specific restriction site. Materials: digested insert DNA and plasmid DNA with the same restriction site, ...)
- 14:00, 26 August 2009 (diff | hist) Team:HKUST/Protocols/gel extraction
- 13:59, 26 August 2009 (diff | hist) N Team:HKUST/Protocols/Ethanol precipitation (New page: 8. Ethanol precipitation Purpose: To concentrate DNA product. Materials: 100% ethanol, 75% ethanol, 3M NaAc, ddH2O, DNA product Procedure: a.Add 2 volume of 100% ethanol. b....)
- 13:59, 26 August 2009 (diff | hist) N Team:HKUST/Protocols/gel extraction (New page: 7. Gel extraction Purpose: To clean up the gel after band cutting from the gel and leave only the desired DNA fragment. Materials: GEX Buffer, WN Buffer, WS Buffer, Elution Buffer (a...)
- 13:58, 26 August 2009 (diff | hist) N Team:HKUST/Protocols/gel cut (New page: 6. Cutting bands on the gel Purpose: To extract specific DNA fragment. Procedure: a.Visualize the bands under UV light. Use long-wavelength UV to minimize damage to the DNA. b...)
- 13:56, 26 August 2009 (diff | hist) N Team:HKUST/Protocols/plasmid extraction (New page: 5. Plamid DNA extraction Purpose: To extract plasmid DNA from E.coli. Materials: MX1 Buffer, MX2 Buffer, MX3 Buffer, WN Buffer, WS Buffer, Elution Buffer (all provided in Mini Plus P...)
- 13:56, 26 August 2009 (diff | hist) N Team:HKUST/Protocols/Enzyme digestion (New page: 4. Enzyme digestion Purpose: To cut the sequence with specific restriction enzyme Materials: substrate DNA, restriction enzymes, corresponding 10X buffer, ddH2O, CIP (Calf Intestinal...)
- 13:55, 26 August 2009 (diff | hist) Team:HKUST/Protocols
- 13:55, 26 August 2009 (diff | hist) N Team:HKUST/Protocols/PCR cleanup (New page: 3. PCR product clean-up Purpose: To clean up primers, dNTPs, enzymes, short-failed PCR products in the PCR reaction. Materials: FAPC Buffer,Wash Buffer, Elution Buffer, PCR Product...)
- 13:54, 26 August 2009 (diff | hist) N Team:HKUST/Protocols/PCR (New page: 2. PCR Purpose: To amplify a specific piece of DNA out from the whole. Materials: ddH2O, 10X ThermoPol Buffer, 1mM dNTP, Forward primer, Reverse Primer, Taq or Vent DNA polymerase, ...)
- 13:54, 26 August 2009 (diff | hist) Team:HKUST/Protocols
- 13:53, 26 August 2009 (diff | hist) Team:HKUST/Protocols
- 13:53, 26 August 2009 (diff | hist) N Team:HKUST/Protocols/Agarose gel (New page: 1. Agarose gel preparation and gel electrophoresis Purpose: To check the result Materials: Agarose, 0.5X TBE, SYBR safe DNA gel stain, DNA loading dye, DNA ladder/marker Choice of...)
- 13:37, 26 August 2009 (diff | hist) Team:HKUST/Project
- 13:37, 26 August 2009 (diff | hist) Team:HKUST/Members
- 13:36, 26 August 2009 (diff | hist) Team:HKUST/Protocols
- 13:34, 26 August 2009 (diff | hist) Team:HKUST/Lab Notebook
- 13:14, 26 August 2009 (diff | hist) Team:HKUST/Lab Notebook
- 09:06, 26 August 2009 (diff | hist) Team:HKUST/Lab Notebook
- 08:30, 26 August 2009 (diff | hist) Team:HKUST/Acknowledgments (Replacing page with '{{HKUST_iGEM_09|}}')
- 08:30, 26 August 2009 (diff | hist) Team:HKUST/Biosafety (Replacing page with '{{HKUST_iGEM_09|}}')
- 08:30, 26 August 2009 (diff | hist) N Team:HKUST/Lab Notebook (New page: {{HKUST_iGEM_09|}})
- 08:30, 26 August 2009 (diff | hist) Team:HKUST/Protocols
- 08:29, 26 August 2009 (diff | hist) Team:HKUST/Project
- 08:29, 26 August 2009 (diff | hist) Team:HKUST/Members
- 08:28, 26 August 2009 (diff | hist) Template:HKUST iGEM 09
- 08:11, 26 August 2009 (diff | hist) Template:HKUST iGEM 09
- 08:10, 26 August 2009 (diff | hist) Template:HKUST iGEM 09
- 08:10, 26 August 2009 (diff | hist) Template:HKUST iGEM 09
- 08:09, 26 August 2009 (diff | hist) Template:HKUST iGEM 09
- 08:08, 26 August 2009 (diff | hist) N File:HK.jpg (top)
- 08:07, 26 August 2009 (diff | hist) Template:HKUST iGEM 09
- 08:06, 26 August 2009 (diff | hist) Template:HKUST iGEM 09
- 07:59, 26 August 2009 (diff | hist) Team:HKUST/Team (→What we did)
(Latest | Earliest) View (newer 250 | older 250) (20 | 50 | 100 | 250 | 500)