http://2009.igem.org/wiki/index.php?title=Special:Contributions/EthanChan&feed=atom&limit=50&target=EthanChan&year=&month=2009.igem.org - User contributions [en]2024-03-29T09:00:27ZFrom 2009.igem.orgMediaWiki 1.16.5http://2009.igem.org/Team:UCSF/NotebookTeam:UCSF/Notebook2009-10-22T03:21:34Z<p>EthanChan: </p>
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<p align="left">In the beginning of the summer our team was divided into two groups - one group focused on Engineering NAVIGATION and the other group focused on Engineering SPEED. The group that engineered NAVIGATION worked with HL60 cells - HL60 Team. The group that engineered SPEED worked with <em>Dictyostelium</em> - Dicty Team.</p><br />
<blockquote><br />
<h2 align="left">HL60 Team</h2><br />
<p align="left"><strong>GOAL</strong>: Our goals for this project are to screen 30 various different GPCR's (G couple protein receptors) to determine which receptors mediate chemotaxis using the high through put transwell assay. After the first phase screening using 5-6 day differentiated HL-60 cell transfected with known receptors is completed, we move on to the secondary phase of screening with viable chemotatic receptors. During the second phase of screening we fused &quot;Actin Modulators&quot;, &quot;PIP3 Modulators&quot;, and &quot;GEF Activators&quot; in hopes of an accelerated chemotatic response. </p><br />
<p align="left">&nbsp;</p><br />
<p align="left"> <strong>ENGINEERING NAVIGATION</strong>:</p><br />
<ul><br />
<li><em>Can we send our cells to new targets?</em> Inserting new Sensors to HL60 (GPCR Screening)</li><br />
<li><em>Can we make our new engineered cells more or less sensitive to the new signals?</em> Tuning receptor sensitivity by attaching recycling modules to the new GPCRs</li><br />
</ul><br />
<p><strong>Milestones</strong>:</p><br />
<p><strong>Step 1.</strong> Clone receptors, N-terminal tags, C-terminal domains. Generate fusions. (<strong>everyone</strong>)<br /><br />
<strong>Step 2.</strong> Transwell screen to identify constructs that affect migration (<strong>Ryan, Eric</strong>)<br /><br />
<strong>Step 3.</strong> Time lapse microscopy to determine directional migration (<strong>Hansi, Jackie, Katja</strong>)<br /><br />
<strong>Step 4.</strong> Establish method for attaching beads to cells (<strong>Katja</strong>)<br /><br />
<p><strong>Team members</strong></p><br />
<p><br />
<a href="https://2009.igem.org/Eric_Wong's_notebook">Eric Wong's notebook</a>: I worked on cloning, PCR, Gel purification and extraction, and was apart of the GPCR screening team.<br /><br />
<a href="https://2009.igem.org/Jackie_Tam's_notebook">Jackie Tam's notebook</a>: I was responsible for cloning, microscopy, and video analysis.<br /><br />
<a href="https://2009.igem.org/Ryan_Liang's_notebook">Ryan Liang's notebook</a>: I cloned parts to be used in the GPCR screens, transfected HL-60 cells with various different GPCRs, ran transwell assays, and analyzed/compared transwell assay results.<br /><br />
<a href="https://2009.igem.org/Cathy_Liu's_notebook">Cathy Liu's notebook</a>: I was one of the main players on team HL-60 who conducted the transwells for the GPCR screen. This included transfections and analysis of data. I was Aynur’s student and Ryan’s and Eric’s buddy. And what a beautiful team we made!<br /><br />
<a href="https://2009.igem.org/Hansi_Liu's_notebook">Hansi Liu's notebook</a>: I established a protocol for time-lapse microscopy of HL-60 cells crawling in gradients, and collected that data.</p><br />
<p>&nbsp;</p><br />
<p align="left"><strong>MAKING CELLS CARRY PAYLOAD</strong>:</p><br />
<ul><br />
<li><em>Can we make our cells carry stuff?</em> - proof-of-concept: attach beads to cells </li><br />
</ul><br />
<p><strong>Team members</strong></p><br />
<p><a href="https://2009.igem.org/Katja_Kolar's_notebook">Katja Kolar's notebook</a>: I did microscopy with wt and hM4D-transfected HL-60 cells, experiments for the &quot;Payload&quot; part of the project, and cloned HL-60 team constructs. </p><br />
<p align="left">&nbsp;</p><br />
<h2 align="left">Dicty Team</h2><br />
<p align="left"><strong>GOAL</strong>: Our goal was to build brakes and accelerators for motile cell. We challenged ourselves to engineer feedback loops that would affect the localization of PIP3 within the cell. </p><br />
<p align="left">&nbsp;</p><br />
<p align="left"> <strong>ENGINEERING SPEED</strong>:</p><br />
<ul><br />
<li><em>Can we send our cells move faster or slower?</em> Creating brakes and accelerators by modulating PIP3 polarity - building synthetic feedback loops by fusing catalytic and localization domains.</li><br />
</ul><br />
<p><strong>Milestones</strong>:</p><br />
<p><strong>Step 1.</strong> PCR parts: catalytic and localization domains <strong>Ryan</strong><br /><br />
<strong>Step 2.</strong> Create combinations of localization domains and catalytic domains (feedback elements) <strong>everyone</strong><br /><br />
<strong>Step 3.</strong> Generate Dictyostelium strains expressing feedback elements <strong>everyone</strong><br /><br />
<strong>Step 4.</strong> Measure motility parameters (speed, directionality) <strong>everyone</strong><br /><br />
<strong>Step 5.</strong> Analyze data. Create histograms and compare if our new feedback elements had any effect on Dicty. Do they cause them to move faster or slower? <strong>everyone</strong></p><br />
<p><strong>Team members</strong></p><br />
<p> <a href="https://2009.igem.org/Allen_Cai's_notebook">Allen Cai's notebook</a>: I worked mostly on the cloning part of the project. I ligated a lot of the localization and catalytic domains together. I also took care of some dicty strains and made movies of them.<br /><br />
<a href="https://2009.igem.org/Alex_Smith's_notebook">Alex Smith's notebook</a>:<br />
I transformed newly engineered constructs into wild type Dictyostelium discoideum, took movies of cells, analyzed data, and briefly experimented with rapamycin mediated recruitment.<br /><br />
<a href="https://2009.igem.org/Edna_Miao's_notebook">Edna Miao's notebook</a>: I transformed new constructs into Dicty, analyzed data, and worked with various different Dicty strains.<br /><br />
<a href="https://2009.igem.org/Ethan_Chan's_notebook">Ethan Chan's notebook</a>: I constructed Dicty cells with new feedback elements, analyzed data, and worked with various different Dicty strains.<br /><br />
<a href="https://2009.igem.org/Ryan_Quan's_notebook">Ryan Quan's notebook</a>: I worked on team dicty and taught the students some basic cloning techniques but mainly learned many new things along with them. </p><br />
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!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}</div>EthanChanhttp://2009.igem.org/Team:UCSF/NotebookTeam:UCSF/Notebook2009-10-22T02:02:53Z<p>EthanChan: </p>
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<p align="left">In the beginning of the summer our team was divided into two groups - one group focused on Engineering NAVIGATION and the other group focused on Engineering SPEED. The group that engineered NAVIGATION worked with HL60 cells - HL60 Team. The group that engineered SPEED worked with <em>Dictyostelium</em> - Dicty Team.</p><br />
<blockquote><br />
<h2 align="left">HL60 Team</h2><br />
<p align="left"><strong>GOAL</strong>: Our goals for this project are to screen 30 various different GPCR's (G couple protein receptors) to determine which receptors mediate chemotaxis using the high through put transwell assay. After the first phase screening using 5-6 day differentiated HL-60 cell transfected with known receptors is completed, we move on to the secondary phase of screening with viable chemotatic receptors. During the second phase of screening we fused &quot;Actin Modulators&quot;, &quot;PIP3 Modulators&quot;, and &quot;GEF Activators&quot; in hopes of an accelerated chemotatic response. </p><br />
<p align="left">&nbsp;</p><br />
<p align="left"> <strong>ENGINEERING NAVIGATION</strong>:</p><br />
<ul><br />
<li><em>Can we send our cells to new targets?</em> Inserting new Sensors to HL60 (GPCR Screening)</li><br />
<li><em>Can we make our new engineered cells more or less sensitive to the new signals?</em> Tuning receptor sensitivity by attaching recycling modules to the new GPCRs</li><br />
</ul><br />
<p><strong>Milestones</strong>:</p><br />
<p><strong>Step 1.</strong> Clone receptors, N-terminal tags, C-terminal domains. Generate fusions. (<strong>everyone</strong>)<br /><br />
<strong>Step 2.</strong> Transwell screen to identify constructs that affect migration (<strong>Ryan, Eric</strong>)<br /><br />
<strong>Step 3.</strong> Time lapse microscopy to determine directional migration (<strong>Hansi, Jackie, Katja</strong>)<br /><br />
<strong>Step 4.</strong> Establish method for attaching beads to cells (<strong>Katja</strong>)<br /><br />
<p><strong>Team members</strong></p><br />
<p><br />
<a href="https://2009.igem.org/Eric_Wong's_notebook">Eric Wong's notebook</a>: I worked on cloning, PCR, Gel purification and extraction, and was apart of the GPCR screening team.<br /><br />
<a href="https://2009.igem.org/Jackie_Tam's_notebook">Jackie Tam's notebook</a>: I was responsible for cloning, microscopy, and video analysis.<br /><br />
<a href="https://2009.igem.org/Ryan_Liang's_notebook">Ryan Liang's notebook</a>: I cloned parts to be used in the GPCR screens, transfected HL-60 cells with various different GPCRs, ran transwell assays, and analyzed/compared transwell assay results.<br /><br />
<a href="https://2009.igem.org/Hansi_Liu's_notebook">Hansi Liu's notebook</a>: I established a protocol for time-lapse microscopy of HL-60 cells crawling in gradients, and collected that data.</p><br />
<p>&nbsp;</p><br />
<p align="left"><strong>MAKING CELLS CARRY PAYLOAD</strong>:</p><br />
<ul><br />
<li><em>Can we make our cells carry stuff?</em> - proof-of-concept: attach beads to cells </li><br />
</ul><br />
<p><strong>Team members</strong></p><br />
<p><a href="https://2009.igem.org/Katja_Kolar's_notebook">Katja Kolar's notebook</a>: I did microscopy with wt and hM4D-transfected HL-60 cells, experiments for the &quot;Payload&quot; part of the project, and cloned HL-60 team constructs. </p><br />
<p align="left">&nbsp;</p><br />
<h2 align="left">Dicty Team</h2><br />
<p align="left"><strong>GOAL</strong>: Our goal was to build brakes and accelerators for motile cell. We challenged ourselves to engineer feedback loops that would affect the localization of PIP3 within the cell. </p><br />
<p align="left">&nbsp;</p><br />
<p align="left"> <strong>ENGINEERING SPEED</strong>:</p><br />
<ul><br />
<li><em>Can we send our cells move faster or slower?</em> Creating brakes and accelerators by modulating PIP3 polarity - building synthetic feedback loops by fusing catalytic and localization domains.</li><br />
</ul><br />
<p><strong>Milestones</strong>:</p><br />
<p><strong>Step 1.</strong> PCR parts: catalytic and localization domains <strong>Ryan</strong><br /><br />
<strong>Step 2.</strong> Create combinations of localization domains and catalytic domains (feedback elements) <strong>everyone</strong><br /><br />
<strong>Step 3.</strong> Generate Dictyostelium strains expressing feedback elements <strong>everyone</strong><br /><br />
<strong>Step 4.</strong> Measure motility parameters (speed, directionality) <strong>everyone</strong><br /><br />
<strong>Step 5.</strong> Analyze data. Create histograms and compare if our new feedback elements had any effect on Dicty. Do they cause them to move faster or slower? <strong>everyone</strong></p><br />
<p><strong>Team members</strong></p><br />
<p> <a href="https://2009.igem.org/Allen_Cai's_notebook">Allen Cai's notebook</a>: I worked mostly on the cloning part of the project. I ligated a lot of the localization and catalytic domains together. I also took care of some dicty strains and made movies of them.<br /><br />
<a href="https://2009.igem.org/Alex_Smith's_notebook">Alex Smith's notebook</a>:<br />
I transformed newly engineered constructs into wild type Dictyostelium discoideum, took movies of cells, analyzed data, and briefly experimented with rapamycin mediated recruitment.<br /><br />
<a href="https://2009.igem.org/Edna_Miao's_notebook">Edna Miao's notebook</a>: I transformed new constructs into Dicty, analyzed data, and worked with various different Dicty strains.<br /><br />
<a href="https://2009.igem.org/Ethan_Chan's_notebook">Ethan Chan's notebook</a>: I constructed Dicty cells with new feedback elements, analyzed data, and worked with various different Dicty strains.<br /><br />
<a href="https://2009.igem.org/Ryan_Quan's_notebook">Ryan Quan's notebook</a>: I worked on team dicty and taught the students some basic cloning techniques but mainly learned many new things along with them. </p><br />
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{| style="color:#333333;background-color:#cccccc;" cellpadding="3" cellspacing="3" border="0" bordercolor="#231f26" width="99%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}</div>EthanChanhttp://2009.igem.org/Alex_SmithAlex Smith2009-10-22T01:57:35Z<p>EthanChan: /* Goofy */</p>
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===Goofy===<br />
<br />
My name is Alexander Ryan Smith and I am a recent graduate of Abraham Lincoln High School in San Francisco. My interest in molecular biology came about when I was introduced to it during a two-year biotechnology program at my high school. During this program, my instructor recommended that I enroll in a tissue culture class at San Francisco City College. I absolutely loved this class because I had the opportunity to differentiate mouse stem cells into heart muscle. I thought it was fascinating the moment I looked through the microscope and observed the sample of beating heart muscle. When my biotechnology instructor noticed my dedication to his class and to the tissue culture class, he became confident that I would be a great member of the UCSF iGEM team. Doing research at UCSF this summer bolstered my interest in science because it allowed me to carry out experiments that were on the cusp of human knowledge. In addition, I attained confidence in the fact that I was given the opportunity to have input along with my peers in this project. Having had the opportunity to design a project at a world-class research university has been a unique experience considering that I just graduated from high school. At the present time, I am attending the University of California at Santa Barbara majoring in biochemistry and molecular biology.</div>EthanChanhttp://2009.igem.org/Team:UCSF/Our_summer_experienceTeam:UCSF/Our summer experience2009-10-22T01:55:30Z<p>EthanChan: /* Personal Blogs about the Experience */</p>
<hr />
<div>== '''Summer Curriculum''' ==<br />
* '''[[Selecting/Development of the iGEM team]]''': High School teacher Mr.Cachianes hand selected seven students from his second year biotechnology class for the iGEM team.<br />
* '''[[Pre-meetings]]''': Starting early April, Mr.Cachianes and his seven students met with UCSF's iGEM coordinators to start the pre-project and learn more about synthetic biology and cell motility.<br />
* '''[[Bootcamp]]''': For two weeks, the iGEM team met everyday for four hour lectures on the mechanisms of cell motility, and discussed scientific papers.<br />
* '''[[Student Brainstorming]]''': Bootcamp culminated in one large brainstorming project where we put our heads together and put what we learned to the test by trying to find ways to brake, accelerate, and steer a cell. <br />
* '''[[Execution]]''': We took the best ideas from brainstorming, modified them, and prepared to carry them out.<br />
*'''[[Curriculum for Teachers]]''' by Saber Khan: Cells as Robots Unit: iGEM/UCSF Summer 2009<br />
<br />
== '''Personal Blogs about the Experience''' ==<br />
* [http://arsigem.blogspot.com/ '''Alex Smith''']:Conducting research at UCSF Mission Bay this summer opened up my eyes to the plethora of fields within biomedical research and it further bolstered my interest in cutting-edge scientific research.<br />
* [http://caiallen.tumblr.com/ '''Allen Cai''']: iGEM gave me hands-on experience on how it would be like to work in a real lab. I loved it and couldn't see myself doing anything else this summer than iGEM.<br />
* [http://cathyliu.tumblr.com/ '''Cathy Liu''']: This summer has been a lot of things. Demanding, stressful, tedious? Sure. But it has definitely been amazing, and a bunch of things I couldn’t express with words. I know we’re going to rock the Jamboree; it’s going to be epic. And I know I’ll only think of all the wonderful times when looking back at all this. There will always be a special place in my heart for UCSF. (P.S. “SUCCESS!”)<br />
* [http://miaomiao.tumblr.com/ '''Edna Miao''']: iGEM has forced me outside my comfort zone many times and consequently has taught me better public speaking and critical thinking skills, on top of new lab techniques of course.<br />
* [http://echan08.blogspot.com/ '''Ethan Chan''']: This experience has brought me closer to my peers and gave me critical lab experience that not many High School students get! <br />
* [http://ertwong.tumblr.com/ '''Eric Wong''']: MADNESS? THIS IS RESEARCH!!! >:O well yeah i enjoyed my time at UCSF, I learned many new things such as lab techniques and chemotaxis as well as meet a bunch of people who i got along great with. CAZZO!!!<br />
* '''Hansi Liu'''<br />
* [http://tackiejam.tumblr.com/ '''Jackie Tam''']: Participating on UCSF’s iGEM team is unparalleled learning experience. This is my first formal lab experience, and I am incredibly thankful that it took place here at the Lim Lab at UCSF.<br />
* '''Katja Kolar''': Mix half a gallon of chemotaxis, mid-sized pack of surface tension gels, big handful of amazing UCSF people, a cup of interesting science, and sprinkle with curious and fun iGEM kids. Bake for 3 months in San Francisco, serve anytime! I loved being part of this summer experience.<br />
* [http://ryanquan.tumblr.com/ '''Ryan Quan''']: This summer I learned many new techniques and how cells move, but the best thing was teaching other people techniques because it really makes you make sure you know your stuff before you open you explain something. <br />
* [http://ryeland.tumblr.com/ '''Ryan Liang''']: This summer has been engagingly educational and highly demanding as well as personally valuable in career-building.<br />
<br />
* [http://davidpincus.tumblr.com/ '''Buddy David Pincus's blog''']<br />
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{| style="color:#333333;background-color:#cccccc;" cellpadding="3" cellspacing="3" border="0" bordercolor="#231f26" width="99%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}</div>EthanChanhttp://2009.igem.org/James_OnufferJames Onuffer2009-10-22T01:51:29Z<p>EthanChan: </p>
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<div>I personally understand the impact that this type of program can have. My 9th grade science teacher volunteered each summer in a research lab at a local community college. She would bring back that experience each year to her own classroom, presenting and demonstrating to the students what she did and implementing parts of this work into her curriculum. As a student, this really awakened in me the impression that science is real and alive, always advancing on unknown questions and changing paths depending on these advances rather than being something that is simply taught from a book where much if not all appears to be already known. This was my motivation for pursuing a career path into research and education; it provided me with an experience that I will never forget. We've done our job if this "light" is also awakened and nourished by the students that participate in our program.</div>EthanChanhttp://2009.igem.org/Team:UCSF/Human_PracticesTeam:UCSF/Human Practices2009-10-22T01:48:47Z<p>EthanChan: </p>
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<div>=='''Balancing Education and Regulation: Teaching Biotechnology and Synthetic Biology in High School'''==<br />
''by Ethan Chan and Edna Miao''<br />
==='''Abstract'''===<br />
<br />
Biotechnology is growing exponentially in popularity due to the great potential of new scientific innovations. Examples include alternative biofuels and lifesaving medical therapies. Biotechnology companies are emerging all over California, especially in the San Francisco Bay Area. As a result, subjects like molecular biology have found their way into high school curricula. At Abraham Lincoln High School in San Francisco, students learn about biotech and its real-world applications in a unique, two-year advanced biotechnology class. A growing number of high schools are creating biotechnology courses. There is particularly strong interest among high schoolers for the cutting edge science of Synthetic Biology.<br />
<br />
The explosion in biotechnology courses in high schools raises important questions about the regulation of biotechnology outside of universities and industry. While it is clearly important to train the future biotechnology workforce, there are concerns among governmental agencies and other groups that unregulated “Do-It-Yourself (DIY) biologists pose a risk to safety and security, especially given the increasing capabilities of Synthetic Biology. One such concern is whether DIY Bio and high school biotechnology courses could potentially release toxic chemical or biological waste into the environment. The safer alternative would be to work in a lab where qualifications must be met and guidelines must be followed. But back in the classroom, what kind of training should be required of teachers? What preparations must be made to ensure safety of the students? There is no definite answer for these questions because biotechnology is a new subject in schools and has yet to be regulated. That doesn’t mean there is no attention being paid to safety. Teachers and members of organizations such as the Bay Area Botechnology Education Consortium (BABEC) often meet to address these questions and to create a safe and ethical high school curriculum for the most beneficial learning experience.<br />
<br />
=='''Section 1 – Biotech classes in High School'''==<br />
<br />
Biotechnology is a booming industry. This relatively new field of science has already been responsible for the birth of many new medicines used today. In fact, many believe that biotechnology has the potential to solve many of our world’s major societal challenges. Biotechnology has already started to appear in high schools across the world. At San Francisco’s Abraham Lincoln High School, the reputation of biotech classes always brings about long waiting lists. Students feel that pursuing biotechnology will result in many different job opportunities. More importantly, having biotech in high schools allows students to gain the basic knowledge required to solve everyday problems. Exposure to molecular biology in high schools motivates students to become future leaders in the biotechnology industry.<br />
<br />
Abraham Lincoln High School offers five first-year biotechnology classes and one second-year biotechnology class annually. Each class is able to hold thirty students. In the first-year’s biotech class, students are taught the fundamentals of using scientific instruments such as pipettes. This hands-on course teaches the same techniques that are used in today’s industry and academic labs. After a few weeks of detailed lectures, students are directed to their lab bench, where they learn how to generate plasmids/recombinant DNA and sequence DNA, along with several other techniques. These experiments are not as simple as following protocols, but actually encourage the students to think creatively. By the end of the first year, students will have the ability to transform bacteria, work with various enzymes, and run gel electrophoresis.<br />
<br />
The second year biotech class expands on the knowledge taught in the first-year. Because of limited resources, only thirty students are chosen from the first-year pool to continue on with the studies. This second course is much more lab intensive. Whereas the majority of first year biotechnology was spent on theory and basic lab skills, second year biotechnology teaches students the applications, implications, and limitations of current biotechnology. Intense lectures accompanied by demos are followed by the labs themselves. The ability to perform each experiment is thoroughly tested by the teacher after every lesson. At the end of the year, students are able to perform techniques such as the Bradford assay, western blotting, sequencing DNA, running ELISAs and purifying proteins. Looking back, the skills that we acquired through second-year biotech played a tremendous role at our time with the iGEM team. <br />
<br />
Skills like protein extraction and ELISA are not ones that can be taught by a teacher who isn’t properly trained. San Francisco has six high schools that offer biotechnology as a class, and Abraham Lincoln High School is fortunate to be one of them. San Mateo High School, located in the Bay Area, is the first to have a three-year program. They even have their own greenhouse! Until recently, biotech courses were virtually non-existent in high schools. With the increasing popularity of biotechnology in high school, there is an increasing need for (and a dearth of) qualified teachers. The interest in more schools having courses like biotech also brings about concerns. Although the benefits are obvious, the effect of having inexperienced teachers educating a class of students could prove to be problematic.<br />
<br />
<br />
=='''Section 2 – Biotech classes in HS Versus DIY BIO'''==<br />
<br />
A teacher interested in teaching biotechnology must have sufficient knowledge of the field of molecular biology. Most of the powerful tools explained in biotech courses expand from the foundations of molecular biology. This advanced subject takes years to master at the graduate level. Currently, the qualifications for new teachers wanting to teach a biotech course have not been established. Instead, the standards for teaching an advanced class such as biotech are the same as any other life science class. This brings up the concern that new teachers are not only undereducated in the content they are teaching, but will also be untrained in the importance of bioethics and safety issues. New companies such as DIY Bio add yet another complication into the mix. Without proper training, there is the potential to create a hazard. In today’s world, the dangers of labs in the garages of people’s homes are enough to alarm the FBI and CIA. Online forums are already available for those who want to do molecular biology at home. Some would doubt the possibility of doing anything dangerous because of the intensive amounts of equipment required. Surprisingly, some of these forums also include instructions on how to get or make your own equipment. With all of these resources easily available to the average person, the government is fully aware of the possibility of devious biohackers in everyday homes. A concern with DIY bio is that “organisms in the hands of amateurs could escape and cause outbreaks of incurable diseases or unpredictable environmental damage” (Jim Thomas of ETC group). Could the dangers of biosecurity also apply to the work being done in high schools? Questions like these are slowly emerging now that the field of biotechnology is increasingly accessible. High schools do have safety standards set by regulators. However, these standards are still perfunctory, and do not take into account the experience and qualifications of teachers. New standards and guidelines need to be developed that will address safety issues while still supporting innovation in the classroom.<br />
<br />
How is the work being done at professional research labs any different from the work being done in high schools or in homes? In science labs at hospitals, universities, and medical schools, the standards are developed by the National Institutes of Health (NIH). These labs usually require sophisticated monitoring equipment. Strict guidelines are put in place to ensure the safety of employees. The NIH has set guidelines from no eating in the lab to requiring certified storage cabinets and biohazard disposal containers. The NIH insists that lab conditions must be kept in a consistent manner. Any violation of these guidelines could result in hefty fines and/or the closure of a lab. Even as temporary employees of UCSF, each iGEM member had to go through a safety certification procedure before we were allowed to do any lab work. The online program consisted of reading about the disposal of dangerous chemicals, emergency procedures, and ‘the rules’ of working in a lab. Our understanding of each rule was assessed with a quiz. Each iGEM team member had to successfully pass the quizzes or any lab work was prohibited. At the classroom level, it is important for educators to have some freedom in creating innovative, new laboratories for students. George Cachianes, the biotechnology teacher at Abraham Lincoln High School, benefits from the current lack of restrictive standards. Having written his own curriculum, Cachianes ensures that his lessons and labs are safe in the high school environment. One of the his top priorities while creating his curriculum has been the safety of his students. As a former employee of the biotech company Genentech, and member of BABEC, Cachianes thinks that not everyone is qualified to pursue a teaching career in science, “Modern applications require new levels of training, not typically common in the requirements to teach high school biotechnology.”<br />
<br />
<br />
=='''Section 3 – The Future of Teachings of Biotech'''==<br />
<br />
This brings about the important question of how to regulate teaching biotech in schools. What should the school requirements be? The safety of the facilities, faculty, and students must be paramount. The safety of the general public outside the classroom must be observed as well. A teacher interested in teaching biotechnology must take into consideration safety and the teaching of bioethics. They need some extra training, and the qualifications for becoming a biotech teacher have not yet been defined. A life science bachelor’s degree is required. With the overwhelming demand for new high school science teachers, some fear that the standards of a bachelor’s degree in science will be lowered in order to make teaching a more accessible career. One option would be taking a very rigorous test that proves the prospective teacher is adequately prepared. Bioethics should certainly be required if teachers plan to teach biotechnology. Mr. Cachianes has often had students debate issues related to biotechnology. At the end of each year, he shows the film “Gattaca,” a powerful movie addressing many ethical issues that can potentially arise from the abuse of biotechnology. <br />
<br />
In addition to learning the applications of biotechnology, students also need to understand the implications of this new technology. For example, there has been much debate in society about Genetically Modified Organisms (GMOs). GMOs come with a long list of benefits and potential concerns, as do many other applications of biotechnology. Students therefore should be educated, and be able to exercise good judgment regarding ethical considerations. In order for that to happen, qualified teachers are needed. BABEC, an organization that includes local biotechnology teachers, meets monthly to address such concerns. People from industry are represented as well. Together, they develop high school biotechnology curricula. These meetings help pool resources by sharing materials and protocols. Furthermore, teachers who come to BABEC workshops learn about the ethical considerations and implications of each application they learn. <br />
<br />
There is a concern that new regulations may stymie the ability of high school students to do research and experiments in classes. However, Mr. Cachianes feels that there will be exceptions for educators that allow them to continue to teach biotechnology. It will, however, be difficult to enforce new regulations on the public. The best way would be to restrict access to purchasing restriction endonucleases and other enzymes that must be purchased from specialty companies. Mr. Cachianes also feels that high schools will remain mostly unaffected, because the experiments performed are made to be safe. It will be possible to have the freedom of teaching a class without having to be burdened by challenging regulations in the future, because if the rules are reasonable then there shouldn’t be a problem. Today, there are already some rules in place. For example, students cannot do any experiments that involve the use of blood. These rules are reasonable, since blood is biohazardous. However, if rules get overly restrictive, Mr. Cachianes has said that they could preclude him from teaching high school students effectively. Hopefully, it will never come to that. Governmental agencies involved in regulating DIY Biology should make sure that the actions they take do not come at the expense of a thriving biotechnology educational system.<br />
<br />
<br />
<br />
{| style="color:#333333;background-color:#cccccc;" cellpadding="3" cellspacing="3" border="0" bordercolor="#231f26" width="99%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}</div>EthanChanhttp://2009.igem.org/Team:UCSF/Human_PracticesTeam:UCSF/Human Practices2009-10-22T01:12:22Z<p>EthanChan: </p>
<hr />
<div>=='''Abstract'''==<br />
<br />
Biotechnology is exponentially growing in popularity over the years due to the great potential of new scientific inventions like alternative biofuels and various lifesaving vaccines. Biotechnology companies are emerging all over the California coast especially the Bay Area. Surprisingly, within this field of science, another source is bustling for attention: High Schools. At Abraham Lincoln High School, students learn about biotech and its real-world applications in an exclusive two-year advanced biotechnology class. The credentials needed to teach a biotechnology course in high school has raised questions as the work is somewhat similar to that of DYI Bio. The issue is that both DYI Bio and high school biotechnology courses can be potentially dangerous as well as hazardous to the environment if an outbreak were to occur. The safer alternative would be to work in a lab where the qualifications must be met and guidelines must be followed. But back in the classroom, what are the teacher’s qualifications? What preparations must be done to ensure safety of the students? There is no definite answer for these questions because biotechnology is a brand new subject in schools and has yet to be regulated. But that doesn’t mean there is no attention brought to it; teachers and members of the BABEC often meet to answer these questions and create a safe and ethical high school curriculum for the most beneficial learning experience.<br />
<br />
=='''Section 1 – Biotech classes in High School'''==<br />
<br />
The world of biotechnology has never been as big as it is now. This fairly new field has already been responsible for the birth of biofuels and many vital vaccines used today. In fact, many believe that biotechnology has the potential to solve many of our everyday challenges. With the promises it is expected to bring, biotech has already started to appear in high schools across the world. At San Francisco’s Abraham Lincoln High School, the hype for this new course always brings about long waiting lists. Students feel that pursuing biotechnology or biotech will result in an influx of different job opportunities. More importantly, having biotech in high schools allow students to gain the baseline knowledge required to solve everyday problems, and learning the material early on allows students to become future leaders in the biotechnology industry.<br />
<br />
Abraham Lincoln High School offers five first-year biotechnology classes and one second-year biotechnology class. Each class is able to hold around thirty students. In first-year biotech, students are taught the fundamentals of using scientific instruments like micropipettes. The hands-on course teaches the very same techniques that are used in today’s industry and academic labs. After a few weeks of detailed lectures, students are directed to their lab bench, and learn how to make plasmids, recombinant DNA, and more. These experiments are not as simple as following protocols, but actually encourage the students to think outside the box. By the end of the first year, students will have the ability to do transformations, work with enzymes and run gel electrophoresis.<br />
<br />
Second year biotech expands on the knowledge taught in first-year. Because of limited resources, thirty students are chosen from the first-year pool to continue on with the studies. This second course is much more lab intensive. Whereas the majority of first year biotechnology was spent on theory and basic lab skills, second year biotechnology teaches students the applications of first year biotechnology. Intense lectures accompanied by demos are followed by the labs themselves. The ability to perform each experiment is thoroughly tested by the teacher after every lesson. At the end of the year, students will be able to perform techniques such as the Bradford assay, western blotting, and protein purifications. Looking back, the skills that we acquired through second-year biotech played a tremendous role at our time with the iGEM team. <br />
Skills like protein extraction and ELISA are not ones that can be taught by a under qualified teacher. San Francisco has three high schools that offer biotechnology as a class, and Abraham Lincoln High School is fortunate to be one of them. San Mateo High School, located in the Bay Area, is the first to have a three-year program; they even have their own greenhouse! Few years prior, biotech courses were practically non-existent. With the popularity of biotech quickly entering the high school doors, more inexperienced teachers are thinking about teaching biotech. The interest of more schools having courses like biotech also brings about concerns. Although the benefits are obvious, the effect of having inexperienced teachers educating a class of students could prove to be risky.<br />
<br />
=='''Section 2 – Biotech classes in HS Versus DIY BIO'''==<br />
<br />
A teacher interested in teaching biotechnology must also have sufficient knowledge in the field of molecular biology. Most of the powerful tools explained in biotech courses expand from the foundations of molecular biology. This complex subject takes years to master even at the graduate level. Currently, the qualifications for new teachers wanting to teach a biotech course are not specifically designed for the course. Instead, the standards for teaching an advance class like biotech are much like any other science class. This brings up the concern that upcoming teachers are not only undereducated in the content they are teaching, but will also avoid the importance of bioethics. While these lessons might sound repetitive, potential dangers can occur if the educator does not stress the importance of it. <br />
<br />
The knowledge given at current high school courses is similar to the work of DIY (Do-It-Yourself) Bio. Although biotech courses are not meant to be dangerous, the basics taught is enough to create a potential hazard. In today’s world, the dangers of hidden labs in the garages of people’s homes are enough to alarm the FBI and CIA. Online forums are already available for those who want to do biology at home. Some would doubt the possibility of doing dangerous labs because of all the equipment required. But surprisingly, some of these forums also include instructions on how to make your own equipment. With all these resources easily available to the average person, the FBI is fully aware of the possibility of biohackers lurking in everyday homes. The concerns with DIY bio is that “organisms in the hands of amateurs could escape and cause outbreaks of incurable diseases or unpredictable environmental damage(Jim Thomas of ETC group).” Could the dangers of biosecurity also apply to the work being done in high schools? Questions like these are slowly coming up now that the field of biotechnology has expanded. Yes, the work being done at the high school level could be considered DIY bio, but they also have standards set by inspectors. However, these standards are still very brief, and with the possibility of inexperienced and under-qualified teachers quickly emerging, these standards deserve to be looked at once again.<br />
<br />
How is the work being done at research labs any different from the ones done at high schools or in public homes? At the high level of science labs at hospitals, universities, and medical schools, the standards are developed by the National Institute of Health (NIH). These labs usually carry a lot of sophisticated equipment, and strict guidelines are put in place to ensure the safety of the employees. NIH has set guidelines from no eating in the lab to allowing only certified storing cabinets to be used. NIH feels that lab conditions must be kept in a consistent matter. Any violations to the guidelines could result in hefty fines and/or the closure of the lab. Even as temporary employees, each iGEM member had to go through a safety certification procedure before we were allowed to do any lab work. The online program consisted of reading about the disposal of dangerous chemicals, emergency procedures, and ‘the rules’ of working in a lab; each reading was wrapped up with a quiz. Each member had to successfully pass the quiz or else any related lab work was prohibited. At the classroom level, it is important for the educator to have some sort of freedom in teaching the class. George Cachianes (biotech teacher) of Abraham Lincoln High School admit that the lack of strict guidelines is one of the best things about being a teacher. Having written his own curriculum, Cachianes ensures that his lessons and labs are safe in a high school environment. One of the top priorities while making his curriculum is the safety of the students. Not having to worry about standards allows him to focus more on a certain topic, rather than being time pressured to move onto another lesson. As a former employee of the biotech giant Genentech, and member of the Bay Area Biotechnology Education Consortium(BABEC), Cachianes admits that not everyone is qualified to pursue a teaching career in science, “But if you haven’t had college and graduate school, you’re not trained to control that kind of environment. My concern is when a teacher only has a bachelor’s degree, they may know how to do it, but they don’t understand how to.” <br />
<br />
=='''Section 3 – The Future of Teachings of Biotech'''==<br />
<br />
This brings about the important questions of how to regulate teaching biotech in schools. What should the school requirements be? The safety of the facilities, faculty, and students must be observed at all times, and the safety of the general public outside the classroom must be observed as well. A teacher interested in teaching biotechnology must take into consideration safety bioethics. They need to be certified, and the qualifications of becoming a biotech teacher have not yet been defined. However, a science related bachelor’s degree is required. Some fear that the standards of a bachelor’s degree in science will be lowered in order to make teaching a more attractive career. Another option would be taking a very rigorous test that proves the prospective teacher is from industry. Bioethics is a must if teachers plan to teach, and Mr.Cachianes used to have debate projects at the end of each year, so students could go into more detail on the ethical sides of biotech. Now he shows Gattaca, a powerful movie discussing many ethical issues that can potentially arise from biotech. <br />
<br />
Aside from basic lab skills such as gel electrophoresis, students should also be learning and discussing the perils and benefits of Biotech. For example, there has been much debate about Genetically Modified Organisms or GMOs. GMOs come with a long list of both benefits and disadvantages, and much of biotech comes with a list of pros and cons. Students therefore should be educated, and be able to exercise good judgment on what they think is right. In order for that to happen, they need qualified teachers. Teachers from the bay area meet once every other month and are in an organization Bay Area Biotechnology Education Consortium or BABEC. People from industry are present as well, and together, they develop a high school curriculum. These meetings help pull resources by sharing materials and protocols. Furthermore, teachers tome to the workshops offered at BABEC to learn the techniques they will teach their students. The issue of ethics is covered at these meetings as well.<br />
<br />
Some fear that the FBI will create rules and regulations that will limit the ability of high schoolers to do research and experiments in classes. However, Mr.Cachianes feels that the FBI will not try to enforce rules that will apply regular citizens, because it will be too difficult to monitor. However, if it was to come down to that, they should try to enforce it at the purchasing level. For example, make buying things like enzymes more difficult by having a legitimate license. Mr.Cachianes also feels that high schools will remain mostly unaffected, because the experiments performed are made to be safe. It will be possible to have the freedom of teaching a class without having to follow rules in the future, because if the rules are reasonable then there shouldn’t be a problem. Today, there are already some rules in place, like students cannot do any experiments that involve their blood. These rules are reasonable, because things like blood are a biohazard. However, if rules get out of hand, Mr.Cachianes has said that he will not be afraid to quit teaching. Hopefully, it will never come down to that.</div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-22T00:50:45Z<p>EthanChan: /* Team Members */</p>
<hr />
<div><!--- The Mission, Experiments ---><br />
<br />
{| style="color:#333333;background-color:#cccccc;" cellpadding="3" cellspacing="3" border="0" bordercolor="#231f26" width="99%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
<br />
{|align="center"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="3"|<br />
<br />
== '''Team Members''' ==<br />
<br />
{| width=100% align="center" border="0" cellpading="20' cellspacing="20"<br />
|'''Students:'''<br />
<br />
*'''High School Student''': [[Allen Cai]]<br />
*'''High School Student''': [[Alex Smith]]<br />
*'''High School Student''': [[Edna Miao]]<br />
*'''High School Student''': [[Ethan Chan]]<br />
*'''High School Student''': [[Eric Wong]]<br />
*'''High School Student''': [[Jackie Tam]]<br />
*'''High School Student''': [[Ryan Liang]]<br />
<br />
<br />
*'''Undergrad Student''': [[Cathy Liu]]<br />
*'''Undergrad Student''': [[Ryan Quan]]<br />
<br />
<br />
*'''International Undergrad Student''': [[Hansi Liu]]<br />
*'''International Undergrad Student''': [[Katja Kolar]]<br />
<br />
|'''Buddies:'''<br />
<br />
*''' Scientific Director''': [[Ben Rhau]]<br />
*''' Scientific Director''': [[Oliver Hoeller]]<br />
<br />
*''' Buddy''': [[Aynur Tasdemir]]<br />
*''' Buddy''': [[Jason Park]] <br />
*''' Buddy''': [[Andrew Houk]] <br />
*''' Buddy''': [[Delquin Gong]] <br />
*''' Buddy''': [[Arthur Millius]] <br />
*''' Buddy''': [[David Pincus]]<br />
*''' Buddy''': [[Bethany Simmons]]<br />
*''' Teacher''': [[Saber Khan]]<br />
<br />
<br />
*''' Program Director''': [[Raquel Gomes]]<br />
*''' Advisor''': [[Wendell Lim]]<br />
*''' Advisor''': [[Orion Weiner]]<br />
*''' Advisor''': [[James Onuffer]]<br />
<br />
*''' Teacher at Lincoln High School''': [[George Cachianes]]<br />
*''' Teacher at Lincoln High School''': [[Julie Reis]]<br />
|}<br />
<br />
<br />
<gallery><br />
Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Kat.jpg|Katja Kolar<br />
</gallery><br />
<br />
<gallery><br />
Buddies<br />
Image:Wendell.JPG|Wendell Lim<br />
Image:Orion_weiner.gif|Orion Weiner<br />
Image:James_onuffer.jpg|James Onuffer<br />
<br />
Image:.|Raquel Gomes<br />
<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery></div>EthanChanhttp://2009.igem.org/File:James_onuffer.jpgFile:James onuffer.jpg2009-10-22T00:49:29Z<p>EthanChan: </p>
<hr />
<div></div>EthanChanhttp://2009.igem.org/Jason_ParkJason Park2009-10-22T00:48:14Z<p>EthanChan: </p>
<hr />
<div>{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
|}<br />
===Volleyball Player===<br />
<br />
My name is Jason Park and I am a 4th year MD/PhD student in the UCSF School of Medicine and the UCSF / UC Berkeley Joint Graduate Group in Bioengineering. I'm originally from the Los Angeles area and went to college at MIT. I've been in the Cell Propulsion Lab for a little over a year and I am co-advised by Dr. Wendell Lim and Dr. Bruce Conklin. I<br />
became a buddy for iGEM because I thought it would be fun working with bright, motivated high school and undergraduate students for the summer and I knew I would get good experience learning to be a better mentor. (Also, I had been interested in participating in iGEM as an undergraduate at MIT but never ended up doing it!) The best thing<br />
about being part of iGEM this summer has been working with and teaching students - together going through the learning process of doing lab research with all of its ups and downs.</div>EthanChanhttp://2009.igem.org/James_OnufferJames Onuffer2009-10-22T00:45:40Z<p>EthanChan: New page: I personally understand the impact that this type of program can have. My 9th grade science teacher volunteered each summer in a research lab at a local community college. She would bring ...</p>
<hr />
<div>I personally understand the impact that this type of program can have. My 9th grade science teacher volunteered each summer in a research lab at a local community college. She would bring back that experience each year to her own classroom, presenting and demonstrating to the students what she did and implementing parts of this work into her curriculum. As a student, this really awakened in me the impression that science is real and alive, always advancing on unknown questions and changing paths depending on these advances rather than being something that is simply taught from a book where much if not all all appears to be already known. This was my motivation for pursuing a career path into research and education; it provided me with an experience that I will never forget. We've done our job if this "light" is also awakened and nourished by the students that participate in our program.</div>EthanChanhttp://2009.igem.org/Hansi_Liu%27s_notebookHansi Liu's notebook2009-10-20T22:00:11Z<p>EthanChan: New page: '''7.22''' posted Aug 19, 2009 1:35 AM by Hansi Liu Transient Cells 1. Empty vector+pMAX CH12=0_CH34=100nMfMLP_CH56=100nMCNO 2. pBR64(hM4)+pMAX CH1=0_CH2=50nMCNO_CH3=1...</p>
<hr />
<div>'''7.22'''<br />
posted Aug 19, 2009 1:35 AM by Hansi Liu<br />
Transient Cells<br />
1. Empty vector+pMAX<br />
CH12=0_CH34=100nMfMLP_CH56=100nMCNO<br />
<br />
2. pBR64(hM4)+pMAX<br />
CH1=0_CH2=50nMCNO_CH3=100nMCNO_CH4=500nMCNO_CH5=1uMCNO_CH6=1.5uMCNO<br />
CH1=0_CH2=50nMCNO_CH3=100nMCNO_CH4=500nMCNO_CH5=1uMCNO_CH6=1.5uMCNO<br />
CH1=0_CH2=50nMCNO_CH3=100nMCNO_CH4=1uMCNO_CH5=1.5uMCNO_CH6=100fMLP<br />
<br />
'''7.31'''<br />
posted Aug 19, 2009 1:22 AM by Hansi Liu<br />
FACS sorted hM4 stable cell line<br />
<br />
hM4 High<br />
1. starve for 1.5hr_CH1=16nMCNO_CH2=48nMCNO_CH3=100nMCNO_CH4=1uMCNO_CH5=1.5uMCNO_CH6=2uMCNO<br />
2. starve for 1.5hr_CH1=16nMCNO_CH2=48nMCNO_CH3=100nMCNO_CH4=1uMCNO_CH5=1.5uMCNO_CH6=2uMCNO<br />
3. starve for 1.5hr_CH12=0_CH34=1uMCNO_CH56=100nMfMLP<br />
<br />
hM4 Low<br />
1. CH1=16nMCNO_CH2=48nMCNO_CH3=100nMCNO_CH4=1uMCNO_CH5=1.5uMCNO_CH6=2uMCNO<br />
2. CH1=16nMCNO_CH2=48nMCNO_CH3=100nMCNO_CH4=1uMCNO_CH5=1.5uMCNO_CH6=2uMCNO<br />
3. CH1=16nMCNO_CH2=48nMCNO_CH3=100nMCNO_CH4=1uMCNO_CH5=1.5uMCNO_CH6=2uMCNO<br />
4. CH23=0_CH456=100nMfMLP<br />
<br />
'''7.27''' <br />
posted '''Aug 19''', 2009 1:18 AM by Hansi Liu<br />
FACS sorted hM4 stable cell lines<br />
hm4High<br />
1. CH12=0_CH34=100nMCNO_CH56=100nMfMLP<br />
2. CH1=0_CH2=16nMCNO_CH3=16nMCNO_CH4=1uMCNO_CH5=5uMCNO_CH6=5uMCNO<br />
3. CH1=0_CH2=16nMCNO_CH3=48nMCNO_CH4=100nMCNO_CH5=1uMCNO_CH6=2uMCNO<br />
4. CH1=0_CH2=16nMCNO_CH3=48nMCNO_CH4=100nMCNO_CH5=1uMCNO_CH6=1.5uMCNO<br />
5. CH1=0_CH2=16nMCNO_CH3=48nMCNO_CH4=100nMCNO_CH5=1uMCNO_CH6=1.5uMCNO<br />
6. CH1=0_CH2=48nMCNO_CH3=100nMCNO_CH4=1uMCNO_CH5=1.5uMCNO_CH6=2uMCNO<br />
7. CH1=0_CH23=16nMCNO_CH4=1uMCNO_CH56=5uMCNO<br />
<br />
'''7.21''' <br />
posted '''Aug 19''', 2009 1:16 AM by Hansi Liu<br />
WT HL-60 <br />
1. CH12=0to0_CH34_0to4nMfMLP_CH56=0to8nMfMLP<br />
2. CH12=0to32nMfMLP_CH34=0to64nMfMLP_CH56=0to80nMfMLP<br />
3. CH1=110nMfMLP_CH2=120nMfMLP_CH3=130nMfMLP_CH4=140nMfMLP_CH5=150nMfMLP_CH6=0<br />
<br />
'''7.20'''<br />
posted '''Aug 19''', 2009 1:14 AM by Hansi Liu [ updated Aug 19, 2009 1:29 AM ]<br />
WT HL-60<br />
CH123=0to0_CH456=0to100nMCNO<br />
Transient Cells<br />
pBR64(hM4)+pMAX<br />
CH1=0_CH2=0to16nMCNO_CH3=0to100nMCNO_CH4=0to160nMCNO_CH5=0to1uMCNO_CH6=0to100nMfMLP<br />
<br />
<br />
'''7.17'''<br />
posted Aug 19, 2009 1:08 AM by Hansi Liu<br />
EZ-Taxiscanning:<br />
WT HL-60<br />
1. CH23=0to0_CH456=0to100nMCNO<br />
2. CH123=0to0_CH456=0to100nMfMLP<br />
<br />
hM4 Stable Cell Line<br />
1. High expression level<br />
CH1=0to0_CH2=0to16nMCNO_CH3=0to48nMCNO_CH4=0to100nMCNO_CH5=0to160nMCNO_CH6=0to1uMCNO<br />
2. Medium expression level<br />
CH1=0to0_CH2=0to16nMCNO_CH3=0to48nMCNO_CH4=0to100nMCNO_CH5=0to160nMCNO_CH6=0to1uMCNO<br />
3. Low expression level<br />
CH1=0to0_CH2=0to16nMCNO_CH3=0to48nMCNO_CH4=0to100nMCNO_CH5=0to160nMCNO_CH6=0to1uMCNO<br />
<br />
'''7.14'''<br />
posted Aug 19, 2009 1:03 AM by Hansi Liu<br />
EZ-Taxiscanning:<br />
<br />
WT HL-60<br />
1. CH123=0to0nMfMLP_CH456=0to100nMfMLP_starve1.5hr<br />
2. CH123=0to0nMfMLP_CH456=0to100nMfMLP_6um chip_starve1hr<br />
3. CH12=0to16nMfMLP_CH34=0to48nMfMLP_CH56=0to160nMfMLP_starve1.5hr<br />
<br />
hM4 Stable Cell Line<br />
CH1=0to0nMCNO_CH2=100pMCNO_CH3=1nMCNO_CH4=10nMCNO_CH5=50nMCNO_CH6=100nMCNO_5uMchip<br />
<br />
'''7.28''' posted Jul 2, 2009 10:45 PM by Hansi Liu [ updated Aug 19, 2009 1:35 AM ]<br />
Transient Cells<br />
<br />
1. Empty vector+pMAX<br />
CH12=0_CH34=1uMCNO_CH56=100fMLP<br />
<br />
2. pBR64(hM4)+pMAX<br />
CH1=0_CH2=50nMCNO_CH3=100nMCNO_CH4=1uMCNO_CH5=2uMCNO_6=5uMCNO<br />
CH1=0_CH2=50nMCNO_CH3=100nMCNO_CH4=1uMCNO_CH5=2uMCNO_6=5uMCNO<br />
<br />
'''7.2''' posted Jul 2, 2009 10:45 PM by Hansi Liu [ updated Jul 3, 2009 12:44 AM ]<br />
11:00<br />
Ready to get an ID card !<br />
<br />
13:30-14:30<br />
Angi taught us how to analysis scan result(cell tracking) using a cool Matlab program written by herself.<br />
<br />
15:00-<br />
Completed the safety training courses on-line. Ready to do experiments~<br />
<br />
later:<br />
Mini Prep: 8 samples from Eric.</div>EthanChanhttp://2009.igem.org/Team:UCSF/NotebookTeam:UCSF/Notebook2009-10-20T21:48:00Z<p>EthanChan: /* Dictyostelium Group */</p>
<hr />
<div>== '''HL60 Group''' ==<br />
* '''Goals:''' Our goals for this project are to screen 30 various different GPCR's (G couple protein receptors)to determine which receptors mediate chemotaxis using the high through put transwell assay. After first phase screening using 5-6 day differentiated HL-60 cell transfected with known receptors is completed, we move on to the secondary stage of screening with viable chemotatic receptors. During the second stage of screening we fused "Actin Modulators", "PIP3 Modulators", and "GEF Activators" in hopes of an accelerated chemotatic response.<br />
*'''''Engineering Navigation:''''' <br />
*''sending cells to new targets - ''GPCR Screening<br />
<br />
*''tuning receptor sensitivity - ''Forced localization of effector proteins to GPCRs<br />
*'''''Payload:''''' <br />
*''can we make our cells carry stuff? - ''attach beads to cells<br />
<br />
<br />
<br />
*[[Cathy Liu's notebook]]: I was one of the main players on team HL-60 who conducted the transwells for the GPCR screen. This included transfections and analysis of data. I was Aynur’s student and Ryan’s and Eric’s buddy. And what a beautiful team we made!<br />
*[[Eric Wong's notebook]]: I worked on cloning, PCR, Gel purification and extraction, and was apart of the GPCR screening team.<br />
*[[Jackie Tam's notebook]]: I was responsible for cloning, microscopy, and video analysis.<br />
*[[Ryan Liang's notebook]]:I cloned parts to be used in the GPCR screens, transfected HL-60 cells with various different GPCRs, ran transwell assays, and analyzed/compared transwell assay results.<br />
*[[Katja Kolar's notebook]]: I did microscopy with wt and hM4D-transfected HL-60 cells, experiments for the "Payload" part of the project, and cloned HL-60 team constructs.<br />
*[[Hansi Liu's notebook]]:<br />
<br />
== '''Dictyostelium Group''' ==<br />
* '''Goals:''' <br />
* '''''Engineering brakes and accelerators:''''' <br />
* ''modulating PIP3 polarity by building synthetic protein based feedback loops''<br />
* workflow:<br />
*1. PCR parts: catalytic and localization domains<br />
*2. Create combinations of localization domains and catalytic domains <br />
*3. Generate Dictyostelium strains expressing feedback elements<br />
*4. Measure motility parameters (speed, directionality)<br />
*3. Analyze data. Create histograms and compare if our plasmid had any effect to dicty.Do they cause them to move faster or slower?<br />
<br />
<br />
<br />
<br />
*[[Allen Cai's notebook]]: I worked mostly on the clonning part of the project. I ligated a lot of the localization and catalytic domains together. I also took care so some dicty strains and made some movies of those dicty strains.<br />
*[[Alex Smith's notebook]]:<br />
*[[Edna Miao's notebook]]: I transformed new constructs into Dicty, analyzed data, and worked with wildtype and PTEN null dicty.<br />
*[[Ethan Chan's notebook]]: Constructed Dicty cells with new parts, analyzed data, and worked with wildtype and PTEN null dicty cells. <br />
*[[Ryan Quan's notebook]]:I worked on team dicty and taught the students some basic cloning techniques but mainly learned many new things along with them.<br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}</div>EthanChanhttp://2009.igem.org/Team:UCSF/NotebookTeam:UCSF/Notebook2009-10-20T21:47:10Z<p>EthanChan: /* Dictyostelium Group */</p>
<hr />
<div>== '''HL60 Group''' ==<br />
* '''Goals:''' Our goals for this project are to screen 30 various different GPCR's (G couple protein receptors)to determine which receptors mediate chemotaxis using the high through put transwell assay. After first phase screening using 5-6 day differentiated HL-60 cell transfected with known receptors is completed, we move on to the secondary stage of screening with viable chemotatic receptors. During the second stage of screening we fused "Actin Modulators", "PIP3 Modulators", and "GEF Activators" in hopes of an accelerated chemotatic response.<br />
*'''''Engineering Navigation:''''' <br />
*''sending cells to new targets - ''GPCR Screening<br />
<br />
*''tuning receptor sensitivity - ''Forced localization of effector proteins to GPCRs<br />
*'''''Payload:''''' <br />
*''can we make our cells carry stuff? - ''attach beads to cells<br />
<br />
<br />
<br />
*[[Cathy Liu's notebook]]: I was one of the main players on team HL-60 who conducted the transwells for the GPCR screen. This included transfections and analysis of data. I was Aynur’s student and Ryan’s and Eric’s buddy. And what a beautiful team we made!<br />
*[[Eric Wong's notebook]]: I worked on cloning, PCR, Gel purification and extraction, and was apart of the GPCR screening team.<br />
*[[Jackie Tam's notebook]]: I was responsible for cloning, microscopy, and video analysis.<br />
*[[Ryan Liang's notebook]]:I cloned parts to be used in the GPCR screens, transfected HL-60 cells with various different GPCRs, ran transwell assays, and analyzed/compared transwell assay results.<br />
*[[Katja Kolar's notebook]]: I did microscopy with wt and hM4D-transfected HL-60 cells, experiments for the "Payload" part of the project, and cloned HL-60 team constructs.<br />
*[[Hansi Liu's notebook]]:<br />
<br />
== '''Dictyostelium Group''' ==<br />
* '''Goals:''' <br />
* '''''Engineering brakes and accelerators:''''' <br />
* ''modulating PIP3 polarity by building synthetic protein based feedback loops''<br />
* workflow:<br />
*1. PCR parts: catalytic and localization domains<br />
*2. Create combinations of localization domains and catalytic domains <br />
*3. Generate Dictyostelium strains expressing feedback elements<br />
*4. Measure motility parameters (speed, directionality)<br />
*3. Analyze data. Create histograms and compare if our plasmid had any effect to dicty.Do they cause them to move faster or slower?<br />
*<br />
<br />
<br />
<br />
*[[Allen Cai's notebook]]: I worked mostly on the clonning part of the project. I ligated a lot of the localization and catalytic domains together. I also took care so some dicty strains and made some movies of those dicty strains.<br />
*[[Alex Smith's notebook]]:<br />
*[[Edna Miao's notebook]]: I transformed new constructs into Dicty, analyzed data, and worked with wildtype and PTEN null dicty.<br />
*[[Ethan Chan's notebook]]: Constructed Dicty cells with new parts, analyzed data, and worked with wildtype and PTEN null dicty cells. <br />
*[[Ryan Quan's notebook]]:I worked on team dicty and taught the students some basic cloning techniques but mainly learned many new things along with them.<br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}</div>EthanChanhttp://2009.igem.org/Team:UCSF/NotebookTeam:UCSF/Notebook2009-10-20T21:46:55Z<p>EthanChan: /* HL60 Group */</p>
<hr />
<div>== '''HL60 Group''' ==<br />
* '''Goals:''' Our goals for this project are to screen 30 various different GPCR's (G couple protein receptors)to determine which receptors mediate chemotaxis using the high through put transwell assay. After first phase screening using 5-6 day differentiated HL-60 cell transfected with known receptors is completed, we move on to the secondary stage of screening with viable chemotatic receptors. During the second stage of screening we fused "Actin Modulators", "PIP3 Modulators", and "GEF Activators" in hopes of an accelerated chemotatic response.<br />
*'''''Engineering Navigation:''''' <br />
*''sending cells to new targets - ''GPCR Screening<br />
<br />
*''tuning receptor sensitivity - ''Forced localization of effector proteins to GPCRs<br />
*'''''Payload:''''' <br />
*''can we make our cells carry stuff? - ''attach beads to cells<br />
<br />
<br />
<br />
*[[Cathy Liu's notebook]]: I was one of the main players on team HL-60 who conducted the transwells for the GPCR screen. This included transfections and analysis of data. I was Aynur’s student and Ryan’s and Eric’s buddy. And what a beautiful team we made!<br />
*[[Eric Wong's notebook]]: I worked on cloning, PCR, Gel purification and extraction, and was apart of the GPCR screening team.<br />
*[[Jackie Tam's notebook]]: I was responsible for cloning, microscopy, and video analysis.<br />
*[[Ryan Liang's notebook]]:I cloned parts to be used in the GPCR screens, transfected HL-60 cells with various different GPCRs, ran transwell assays, and analyzed/compared transwell assay results.<br />
*[[Katja Kolar's notebook]]: I did microscopy with wt and hM4D-transfected HL-60 cells, experiments for the "Payload" part of the project, and cloned HL-60 team constructs.<br />
*[[Hansi Liu's notebook]]:<br />
<br />
== '''Dictyostelium Group''' ==<br />
* '''Goals:''' <br />
* '''''Engineering brakes and accelerators:''''' <br />
* ''modulating PIP3 polarity by building synthetic protein based feedback loops''<br />
* workflow:<br />
*1. PCR parts: catalytic and localization domains<br />
*2. Create combinations of localization domains and catalytic domains <br />
*3. Generate Dictyostelium strains expressing feedback elements<br />
*4. Measure motility parameters (speed, directionality)<br />
*3. Analyze data. Create histograms and compare if our plasmid had any effect to dicty.Do they cause them to move faster or slower?<br />
*<br />
<br />
><br />
*[[Allen Cai's notebook]]: I worked mostly on the clonning part of the project. I ligated a lot of the localization and catalytic domains together. I also took care so some dicty strains and made some movies of those dicty strains.<br />
*[[Alex Smith's notebook]]:<br />
*[[Edna Miao's notebook]]: I transformed new constructs into Dicty, analyzed data, and worked with wildtype and PTEN null dicty.<br />
*[[Ethan Chan's notebook]]: Constructed Dicty cells with new parts, analyzed data, and worked with wildtype and PTEN null dicty cells. <br />
*[[Ryan Quan's notebook]]:I worked on team dicty and taught the students some basic cloning techniques but mainly learned many new things along with them.<br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}</div>EthanChanhttp://2009.igem.org/Team:UCSF/NotebookTeam:UCSF/Notebook2009-10-20T21:45:33Z<p>EthanChan: /* HL60 Group */</p>
<hr />
<div>== '''HL60 Group''' ==<br />
* '''Goals:''' Our goals for this project are to screen 30 various different GPCR's (G couple protein receptors)to determine which receptors mediate chemotaxis using the high through put transwell assay. After first phase screening using 5-6 day differentiated HL-60 cell transfected with known receptors is completed, we move on to the secondary stage of screening with viable chemotatic receptors. During the second stage of screening we fused "Actin Modulators", "PIP3 Modulators", and "GEF Activators" in hopes of an accelerated chemotatic response.<br />
*'''''Engineering Navigation:''''' <br />
*''sending cells to new targets - ''GPCR Screening<br />
<br />
*''tuning receptor sensitivity - ''Forced localization of effector proteins to GPCRs<br />
*'''''Payload:''''' <br />
*''can we make our cells carry stuff? - ''attach beads to cells<br />
<br />
*[[Cathy Liu's notebook]]: I was one of the main players on team HL-60 who conducted the transwells for the GPCR screen. This included transfections and analysis of data. I was Aynur’s student and Ryan’s and Eric’s buddy. And what a beautiful team we made!<br />
*[[Eric Wong's notebook]]: I worked on cloning, PCR, Gel purification and extraction, and was apart of the GPCR screening team.<br />
*[[Jackie Tam's notebook]]: I was responsible for cloning, microscopy, and video analysis.<br />
*[[Ryan Liang's notebook]]:I cloned parts to be used in the GPCR screens, transfected HL-60 cells with various different GPCRs, ran transwell assays, and analyzed/compared transwell assay results.<br />
*[[Katja Kolar's notebook]]: I did microscopy with wt and hM4D-transfected HL-60 cells, experiments for the "Payload" part of the project, and cloned HL-60 team constructs.<br />
*[[Hansi Liu's notebook]]:<br />
<br />
== '''Dictyostelium Group''' ==<br />
* '''Goals:''' <br />
* '''''Engineering brakes and accelerators:''''' <br />
* ''modulating PIP3 polarity by building synthetic protein based feedback loops''<br />
* workflow:<br />
*1. PCR parts: catalytic and localization domains<br />
*2. Create combinations of localization domains and catalytic domains <br />
*3. Generate Dictyostelium strains expressing feedback elements<br />
*4. Measure motility parameters (speed, directionality)<br />
*3. Analyze data. Create histograms and compare if our plasmid had any effect to dicty.Do they cause them to move faster or slower?<br />
*<br />
<br />
><br />
*[[Allen Cai's notebook]]: I worked mostly on the clonning part of the project. I ligated a lot of the localization and catalytic domains together. I also took care so some dicty strains and made some movies of those dicty strains.<br />
*[[Alex Smith's notebook]]:<br />
*[[Edna Miao's notebook]]: I transformed new constructs into Dicty, analyzed data, and worked with wildtype and PTEN null dicty.<br />
*[[Ethan Chan's notebook]]: Constructed Dicty cells with new parts, analyzed data, and worked with wildtype and PTEN null dicty cells. <br />
*[[Ryan Quan's notebook]]:I worked on team dicty and taught the students some basic cloning techniques but mainly learned many new things along with them.<br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}</div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T21:44:03Z<p>EthanChan: /* Team Members */</p>
<hr />
<div>{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="3"|<br />
<br />
== '''Team Members''' ==<br />
<br />
{| width=100% align="center" border="0" cellpading="20' cellspacing="20"<br />
|'''Students:'''<br />
<br />
*'''High School Student''': [[Allen Cai]]<br />
*'''High School Student''': [[Alex Smith]]<br />
*'''High School Student''': [[Edna Miao]]<br />
*'''High School Student''': [[Ethan Chan]]<br />
*'''High School Student''': [[Eric Wong]]<br />
*'''High School Student''': [[Jackie Tam]]<br />
*'''High School Student''': [[Ryan Liang]]<br />
<br />
<br />
*'''Undergrad Student''': [[Cathy Liu]]<br />
*'''Undergrad Student''': [[Ryan Quan]]<br />
<br />
<br />
*'''International Undergrad Student''': [[Hansi Liu]]<br />
*'''International Undergrad Student''': [[Katja Kolar]]<br />
<br />
|'''Buddies:'''<br />
<br />
*''' Advisor''': [[Wendell Lim]]<br />
*''' Advisor''': [[Orion Weiner]]<br />
*''' Advisor''': [[James Onuffer]]<br />
*''' Scientific Director''': [[Ben Rhau]]<br />
*''' Scientific Director''': [[Oliver Hoeller]]<br />
*''' Education Development''': [[Raquel Gomes]]<br />
*''' Buddy''': [[Aynur Tasdemir]]<br />
*''' Buddy''': [[Jason Park]] <br />
*''' Buddy''': [[Andrew Houk]] <br />
*''' Buddy''': [[Delquin Gong]] <br />
*''' Buddy''': [[Arthur Millius]] <br />
*''' Buddy''': [[David Pincus]]<br />
*''' Buddy''': [[Bethany Simmons]]<br />
*''' Buddy''': [[Saber Khan]]<br />
|}<br />
<br />
<gallery><br />
Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery><br />
<br />
<gallery><br />
Buddies<br />
Image:Wendell.JPG|Wendell Lim<br />
Image:Orion_weiner.gif|Orion Weiner<br />
Image:.|James Onuffer<br />
<br />
Image:.|Raquel Gomes<br />
<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery></div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T21:41:09Z<p>EthanChan: /* Team Members */</p>
<hr />
<div>{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="3"|<br />
<br />
== '''Team Members''' ==<br />
<br />
{| width=100% align="center" border="0" cellpading="20' cellspacing="20"<br />
|'''Students:'''<br />
<br />
*'''High School Student''': [[Allen Cai]]<br />
*'''High School Student''': [[Alex Smith]]<br />
*'''High School Student''': [[Edna Miao]]<br />
*'''High School Student''': [[Ethan Chan]]<br />
*'''High School Student''': [[Eric Wong]]<br />
*'''High School Student''': [[Jackie Tam]]<br />
*'''High School Student''': [[Ryan Liang]]<br />
<br />
<br />
*'''Undergrad Student''': [[Cathy Liu]]<br />
*'''Undergrad Student''': [[Ryan Quan]]<br />
<br />
<br />
*'''International Undergrad Student''': [[Hansi Liu]]<br />
*'''International Undergrad Student''': [[Katja Kolar]]<br />
<br />
<br />
|'''Buddies:'''<br />
<br />
*''' Advisor''': [[Wendell Lim]]<br />
*''' Advisor''': [[Orion Weiner]]<br />
*''' Advisor''': [[James Onuffer]]<br />
*''' Scientific Director''': [[Ben Rhau]]<br />
*''' Scientific Director''': [[Oliver Hoeller]]<br />
*''' Education Development''': [[Raquel Gomes]]<br />
*''' Buddy''': [[Aynur Tasdemir]]<br />
*''' Buddy''': [[Jason Park]] <br />
*''' Buddy''': [[Andrew Houk]] <br />
*''' Buddy''': [[Delquin Gong]] <br />
*''' Buddy''': [[Arthur Millius]] <br />
*''' Buddy''': [[David Pincus]]<br />
*''' Buddy''': [[Bethany Simmons]]<br />
*''' Buddy''': [[Saber Khan]]<br />
|}<br />
<br />
<gallery><br />
Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery><br />
<br />
<gallery><br />
Buddies<br />
Image:Wendell.JPG|Wendell Lim<br />
Image:Orion_weiner.gif|Orion Weiner<br />
Image:.|James Onuffer<br />
<br />
Image:.|Raquel Gomes<br />
<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery></div>EthanChanhttp://2009.igem.org/Orion_WeinerOrion Weiner2009-10-20T21:39:23Z<p>EthanChan: New page: I’m an assistant professor in the Cardiovascular Research Institute and the Dept of Biochemistry at UCSF. I’ve had my own lab at UCSF for 4yrs but have been researching how cells move...</p>
<hr />
<div>I’m an assistant professor in the Cardiovascular Research Institute and the Dept of Biochemistry at UCSF. I’ve had my own lab at UCSF for 4yrs but have been researching how cells move in response to external cues since I started I first started working with these fascinating cells in grad school. My lab has been heavily involved in science outreach through programs such as the Science and Health Education Partnership What attracted me to Igem was the opportunity to give motivated talented high school students hands-on experience in cutting edge synthetic biology research. The most rewarding portion of the Igem experience is the energy and excitement these terrific Igem students have brought to the lab— they have catalyzed several productive projects that we would not have otherwise pursued.</div>EthanChanhttp://2009.igem.org/Further_ResultsFurther Results2009-10-20T21:37:39Z<p>EthanChan: New page: Here are the actual data and controls for the brake we describe on the previous page. For each strain at least 3 movies were taken on different days (total of 60-200 cells). After tracking...</p>
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<div>Here are the actual data and controls for the brake we describe on the previous page. For each strain at least 3 movies were taken on different days (total of 60-200 cells). After tracking, the average speed and standard deviation for each strain was measured and a student's t-test performed to test whether differences were significant.<br />
<br />
[[Image:Slide29.jpg|600px|thumb|left|'''Speed of movement'''. ''Dictyostelium'' cells expressing the negative feedback element PTEN-RasCda move significantly (p<0.0001) slower than wildtype (Ax2). Control strains expressing localization (PTEN) or catalytic (RasC da) domains only show no significant effect.]]</div>EthanChanhttp://2009.igem.org/Team:UCSF/ProjectTeam:UCSF/Project2009-10-20T21:37:22Z<p>EthanChan: /* Results: */</p>
<hr />
<div>{|align="justify"<br />
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<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
<br />
== '''Overall project''' ==<br />
<br />
Your abstract<br />
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== '''Part 1: NAVIGATION: Rewiring the cell to move toward new chemical signals'''==<br />
<br />
=== Motivation: ''Why is this useful?'' ===<br />
We envision a cellular robot that could travel to practically any site in the human body. This would provide a flexible platform that could be used for a variety of therapeutic tasks. The first step toward achieving this goal is to broaden the range of possible chemotactic targets for our cells. Ideally, we could connect virtually any input to chemotaxis in a generalized way.<br />
<br />
=== Approach: ===<br />
Neutrophils sense most of their chemotactic signals through [http://en.wikipedia.org/wiki/G_protein_coupled_receptor G protein-coupled receptors] (GPCRs). The spectrum of chemical signals to which these cells respond is therefore determined, at least in part, by the set of GPCRs they express. Can this spectrum be broadened arbitrarily by the introduction of new GPCRs? We tested this idea by transiently expressing [[23 exogenous GPCRs]] in HL-60 (neutrophil-like) cells.<br />
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[[Image:new_targets.jpg|350px|thumb|center|'''Inserting new sensors:''' Exogenous GPCRs were expressed in HL-60 cells.]]<br />
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These cells were then tested for their ability to migrate toward ligands for the new GPCRs in multiwell [http://en.wikipedia.org/wiki/Chemotaxis_assay#Two-chamber_techniques Boyden chamber] assays. We measured the fold change in % of cells migrating toward the new ligand (with vs without added GPCR) at the peak response. We refer to this ratio as the "Migration Index." For receptors that appeared to activate a migration response (Migration Index > 3), we also conducted time-lapse microscopy to determine whether the cell movement was directed toward the gradient of ligand.<br />
<br />
=== Results: ===<br />
6 of the GPCRs we transiently expressed in our cells resulted in a Migration Index > 3.<br />
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[[Image:Fold_Changes.png|650px|thumb|center|'''Chasing new targets:''' HL-60 cells migrate to new GPCR ligands]]<br />
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Here, we show an example of one of these receptors (M3/M2 chimera) mediating directional migration up a stable, linear gradient of ligand. Transfected cells are fluorescent, and the concentration of ligand increases in the direction corresponding to the top of the image:<br />
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<html><object width="560" height="340"><param name="movie" value="http://www.youtube.com/v/rB0QQlfa7f4&hl=en&fs=1&"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="http://www.youtube.com/v/rB0QQlfa7f4&hl=en&fs=1&" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="560" height="340"></embed></object></html><br />
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''Common characteristics of chemotaxis receptors:'' All 6 receptors we identified couple to the [http://en.wikipedia.org/wiki/Gi_alpha_subunit Gi] signaling pathway. The behavior of the M3/M2 chimera, however, suggests that it may be possible to convert receptors with different coupling specificities into chemotaxis receptors. To generate this chimera, the third intracellular loop (i3) from the M3 muscarinic acetylcholine receptor ([http://en.wikipedia.org/wiki/Gq_alpha_subunit Gq] coupled) was exchanged with that of M2 muscarinic receptor (Gi coupled). It has previously been shown that this chimera now couples to Gi.<br />
<br />
[[Image:chimera.png|350px|thumb|center|i3 loop determines the coupling specificty of the M3 muscarinic acetylcholine receptor.]]<br />
<br />
<br />
Why the i3 loop allows the M3/M2 chimeric receptor to signal to the cell's chemotaxis machinery remains a question for further study. However, the possibility exists that more Gq-coupled (and possibly [http://en.wikipedia.org/wiki/Gs_alpha_subunit Gs]-coupled) receptors could be converted in this way, thus dramatically increasing the number of potential chemotaxis targets.<br />
<br />
== '''Part 2: NAVIGATION: Tuning receptor sensitivity'''==<br />
<br />
=== Motivation: ''Why is this useful?'' ===<br />
In our experiments, we could control exactly how much ligand was presented to our cells. In "real-life," however, we would want our cellular robots to be able to respond to a variety of signal strengths: from very low to very high. To accomplish this, we would want to be able to control the ''sensitivity'' of our receptors, or how the receptor's output changes when the measured quantity of ligand changes.<br />
<br />
=== Approach: ===<br />
We felt that one key determinant of sensitivity would be the number of receptors present at the plasma membrane of the cell. Therefore, we measured the migration response of a receptor (delta Opioid receptor) whose recycling behavior could be engineered by fusing different recycling interaction modules to the C terminus of the GPCR. We tested a number of such receptor-module fusions for migration response and compared them to receptor alone. The primary assay here was again the Boyden chamber (transwell) assay.<br />
<br />
=== Results: ===<br />
We found that virtually any protein domain/module known to alter the recycling of delta Opioid receptor (DOR) affected cellular migration to a low concentration of ligand (1 nM DADLE). Below, we show two examples of such domains. The actin binding domain from alpha-Actinin-1 (ACTN1ABD) appears to potentiate cellular migration when compared to the wild-type receptor. On the other hand, cellular response is inhibited at this concentration when DOR is bound to a domain of EBP50 (ERMbd) that binds to ERM (ezrin/radixin/moesin) family of proteins.<br />
<br />
[[Image:Sensitivity.png|350px|thumb|center|Recycling modules affect sensitivity of DOR]]<br />
<br />
== '''Part 3: SPEED: Engineering accelerators and brakes'''== <br />
'''A cellular cruise control by modulating cell polarity with feedback loops''' <br />
<br />
=== Motivation: ''why is this useful?'' ===<br />
Just like we have control over speed in a car – we can brake or accelerate – it would be useful to engineer such behavior into our cellular nanorobots.<br />
Just think about it: We could '''speed cells up''' so that they reach their targets faster and '''stop them''' once they have arrived or do not behave properly.<br />
<br />
=== Background: ===<br />
For these experiments we chose ''Dictyostelium discoideum'' cells to test our prototypical brakes and accelerators quickly. We expect that our brakes and accelerators can be used in a plug and play fashion because Dicty’s way of movement is very similar to a neutrophil’s:<br />
<br />
When a receptor binds chemoattractant, it induces the conversion of PhosphatidylInositol(4,5)bisphosphate (PIP2) to PhosphatidylInositol(3,4,5)trisphosphate (PIP3) (two signaling lipids in the plasma membrane) at the front of our cells. In a '''positive feedback loop''' PIP3 triggers the formation of more PIP3 at the front while similarly PIP2 leads to more PIP2 production at the sides and rear of the cell. This system sets the axis of polarity of the cell. The PIP3 patch at the front functions as a ‘turbo boost’ pushing the cell forward.<br />
<br />
<br />
[[Image:BACKGROUND1.jpg|400px|thumb|center|'''Polarized distribution of PIP3 and PIP2:''' A patch of PIP3 is localized at the front of a cell while PIP2 is at the back. Feedback loops are used to establish and maintain this polarized distribution. ]]<br />
<br />
=== Approach: ===<br />
Inspired by nature we tried to build accelerators and brakes by introducing our own synthetic protein based feedback loops. We designed feedback elements by fusing localization and catalytic domains involved in PIP3 production and degradation to modulate localization and level of PIP3 and PIP2 in the cell. <br />
<br />
Here is an example of a positive feedback loop: a PIP3 binding localization domain fused to a PIP3 producing catalytic domain could produce more PIP3 where there is already PIP3- at the front. This might strengthen polarity and accelerate a cell.<br />
<br />
[[Image:positive_feedback_loop2.jpg|450px|thumb|center|'''Synthetic protein based feedback loops:''' One example: a positive feedback loop comprising a PIP3 binding domain fused to a PIP3 generating enzyme.]]<br />
<br />
=== Results: ===<br />
Over the summer we assembled more than 100 fusions of localization and catalytic domains and screened whether they work. How? We measured the effect our constructs have on motility of ''Dictyostelium'' cells: stronger polarity should make cells faster while weaker polarity ought to slow them down!<br />
<br />
[[Matrix of Fusion Constructs|Here]] is an overview of all feedback loops we screened and the effect they had on the speed of cells. <br />
We used automated cell tracking on more than 196 hours worth of movies (note: one movie is 10 minutes!) and identified strains that moved faster or slower at a very stringent statistical cutoff (p<0.0001). <br />
<br />
<br />
This way we were able to identify '''7 brakes and 1 accelerator'''!<br />
<br />
Check out the movie of '''one of our strong brakes (PTEN-RasC dominant active (da))[http://www.youtube.com/watch?v=_9od33Nx06Y] compared to wildtype [http://www.youtube.com/watch?v=Vtdtf8-zSRs].''' <br />
<br />
<br />
[[Image:HO230.jpg|400px|thumb|right|'''PTEN fused to RasC da (PIP2 binding - PIP3 generating); speed: 3.5 um/min''' Cells were plated in buffer and basic motile behavior was recorded for 10 minutes taking 1 picture every 15 seconds.]] [[Image:wt.jpg|400px|thumb|left|'''wildtype; speed: 5.9 um/min'''<br />
Cells were plated in buffer and basic motile behavior was recorded for 10 minutes taking 1 picture every 15 seconds.]]<br />
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We hypothesize that this construct acts as a '''negative feedback loop on PIP2''' - (generating PIP3 where PIP2 should be) thereby confusing the cell with multiple fronts:<br />
<br />
[[Image:brake3.jpg|650px|center]]<br />
<br />
This is indeed an effect of fusing the particular localization to the catalytic domain as neither of them alone has such a strong effect ([[Further Results|see details]]).<br />
<br />
=== Summary and outlook: ===<br />
We have screened more than 100 synthetic feedback elements for their ability to accelerate or slow down speed of cell motility. We have isolated a hand full of functional elements. Now we need to confirm the mechanism of action of these elements. In the future we would like to make them inducible by a signal from outside – like a stoplight!<br />
<br />
=Methods=<br />
<br />
*[[Media:Aar1_shuffle.pdf|Cloning: Aar1 Shuffle]] - besides BBFRFC28, here is an actual step by step scheme we used for some of our combinatorial assembly of parts<br />
*[[Media:DICTY TRANSFORMATION.pdf|Dicty: Transformation Protocol]] - here is how we get our constructs into Dicty<br />
*[[Media:DICTY-analyzing_motility.pdf|Dicty: Motility Assay Protocol]] - here is how we prepare and film Dicty cells<br />
<br />
=Selected Reading=<br />
<br />
<br />
'''Engineering Speed:'''<br />
<br />
G protein signaling events are activated at the leading edge of chemotactic cells. Parent CA, Blacklock BJ, Froehlich WM, Murphy DB, Devreotes PN. Cell. 1998 Oct 2;95(1):81-91.<br />
<br />
PI3-kinase signaling contributes to orientation in shallow gradients and enhances speed in steep chemoattractant gradients. Bosgraaf L, Keizer-Gunnink I, Van Haastert PJ. J Cell Sci. 2008 Nov 1;121(Pt 21):3589-97. Epub 2008 Oct 7.</div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T21:31:39Z<p>EthanChan: /* Team Members */</p>
<hr />
<div>{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="3"|<br />
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== '''Team Members''' ==<br />
<br />
{| width=100% align="center" border="0" cellpading="20' cellspacing="20"<br />
|'''Students:'''<br />
<br />
*'''High School Student''': [[Allen Cai]]<br />
*'''High School Student''': [[Alex Smith]]<br />
*'''High School Student''': [[Edna Miao]]<br />
*'''High School Student''': [[Ethan Chan]]<br />
*'''High School Student''': [[Eric Wong]]<br />
*'''High School Student''': [[Jackie Tam]]<br />
*'''High School Student''': [[Ryan Liang]]<br />
<br />
<br />
*'''Undergrad Student''': [[Cathy Liu]]<br />
*'''Undergrad Student''': [[Ryan Quan]]<br />
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*'''International Undergrad Student''': [[Hansi Liu]]<br />
*'''International Undergrad Student''': [[Katja Kolar]]<br />
<br />
<br />
|'''Buddies:'''<br />
<br />
*''' Advisor''': [[Wendell Lim]]<br />
*''' Advisor''': [[Orion Weiner]]<br />
*''' Advisor''': [[James Onuffer]]<br />
*''' Scientific Director''': [[Ben Rhau]]<br />
*''' Scientific Director''': [[Oliver Hoeller]]<br />
*''' Education Development''': [[Raquel Gomes]]<br />
*''' Buddy''': [[Aynur Tasdemir]]<br />
*''' Buddy''': [[Jason Park]] <br />
*''' Buddy''': [[Andrew Houk]] <br />
*''' Buddy''': [[Delquin Gong]] <br />
*''' Buddy''': [[Arthur Millius]] <br />
*''' Buddy''': [[David Pincus]]<br />
*''' Buddy''': [[Bethany Simmons]]<br />
*''' Buddy''': [[Saber Khan]]<br />
|}<br />
<br />
<br />
<gallery><br />
Buddies<br />
Image:Wendell.JPG|Wendell Lim<br />
Image:Orion_weiner.gif|Orion Weiner<br />
Image:.|James Onuffer<br />
<br />
Image:.|Raquel Gomes<br />
<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery><br />
<br />
<br />
<gallery><br />
Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery></div>EthanChanhttp://2009.igem.org/Matrix_of_Fusion_ConstructsMatrix of Fusion Constructs2009-10-20T20:49:57Z<p>EthanChan: New page: We engineered and screened a large '''matrix of fusion constructs''' this summer: Each construct consists of one catalytic domain (rows) fused to one localization domain (columns). We arr...</p>
<hr />
<div>We engineered and screened a large '''matrix of fusion constructs''' this summer:<br />
<br />
Each construct consists of one catalytic domain (rows) fused to one localization domain (columns). We arranged them in categories so that it is easier to think about what we expect them to do. Each field represents a fusion construct (e.g.: localize to the front - make more PIP3; localize to the back- make more PIP2...).<br />
If you are interested what all the elements actually are check out our biobricks.<br />
<br />
<br />
When you look at the matrix concentrate on colored fields - white fields simply indicate constructs we did not complete cloning over the summer :-(. <br />
The colored fields however show constructs we cloned and also tested in cells. Here is what the colors mean:<br />
<br />
Fields in 'tan' show constructs that do not significantly affect speed when compared to wildtype.<br />
Actual hits are in green and red:<br />
Fusion constructs in '''green''' act as '''accelerators''' causing cells to move significantly faster than wildtype. Any construct in '''red''' acts as '''brakes''', slowing cells down compared to wildtype.<br />
Quite some hits fall in the last column and the last row - that's where additional controls are located (localization or catalytic domains only).<br />
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[[Image:screen3.jpg|700px|thumb|center|]]<br />
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When we compare our hits with all the appropriate controls (making sure that the fusions cause an effect but not overexpression of the catalytic or localization domain alone) we can conclude that we '''identified 7 brakes and 1 accelerator''': <br />
<br />
''' Brakes:'''<br />
BBa_K209063: PTEN-PI3K1(w/o Nterm)<br />
BBa_K209065: PTEN-SHIP1<br />
BBa_K209066: PTEN-RasC da<br />
BBa_K209069: PTEN-NFA<br />
BBa_K209036: PH(PLC)-SHIP1<br />
BBa_K209091: ACA-SHIP1<br />
BBa_K209093: ACA-RasGda<br />
<br />
''' Accelerator:'''<br />
BBa_K209049: RBD(PI3K1)-RasG da)</div>EthanChanhttp://2009.igem.org/Team:UCSF/ProjectTeam:UCSF/Project2009-10-20T20:49:41Z<p>EthanChan: /* Results: */</p>
<hr />
<div>{|align="justify"<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
<br />
== '''Overall project''' ==<br />
<br />
Your abstract<br />
<br />
== '''Part 1: Navigation: Rewiring the cell to move toward new chemical signals'''==<br />
<br />
=== Motivation: ''Why is this useful?'' ===<br />
We envision a cellular robot that could travel to practically any site in the human body. This would provide a flexible platform that could be used for a variety of therapeutic tasks. The first step toward achieving this goal is to broaden the range of possible chemotactic targets for our cells. Ideally, we could connect virtually any input to chemotaxis in a generalized way.<br />
<br />
=== Approach: ===<br />
Neutrophils sense most of their chemotactic signals through [http://en.wikipedia.org/wiki/G_protein_coupled_receptor G protein-coupled receptors] (GPCRs). The spectrum of chemical signals to which these cells respond is therefore determined, at least in part, by the set of GPCRs they express. Can this spectrum be broadened arbitrarily by the introduction of new GPCRs? We tested this idea by transiently expressing [[23 exogenous GPCRs]] in HL-60 (neutrophil-like) cells.<br />
<br />
<br />
[[Image:new_targets.jpg|350px|thumb|center|'''Inserting new sensors:''' Exogenous GPCRs were expressed in HL-60 cells.]]<br />
<br />
<br />
These cells were then tested for their ability to migrate toward ligands for the new GPCRs in multiwell [http://en.wikipedia.org/wiki/Chemotaxis_assay#Two-chamber_techniques Boyden chamber] assays. We measured the fold change in % of cells migrating toward the new ligand (with vs without added GPCR) at the peak response. We refer to this ratio as the "Migration Index." For receptors that appeared to activate a migration response (Migration Index > 3), we also conducted time-lapse microscopy to determine whether the cell movement was directed toward the gradient of ligand.<br />
<br />
=== Results: ===<br />
6 of the GPCRs we transiently expressed in our cells resulted in a Migration Index > 3.<br />
<br />
<br />
[[Image:Fold_Changes.png|650px|thumb|center|'''Chasing new targets:''' HL-60 cells migrate to new GPCR ligands]]<br />
<br />
<br />
<br />
Here, we show an example of one of these receptors (M3/M2 chimera) mediating directional migration up a stable, linear gradient of ligand. Transfected cells are fluorescent, and the concentration of ligand increases in the direction corresponding to the top of the image:<br />
<br />
<br />
<html><object width="560" height="340"><param name="movie" value="http://www.youtube.com/v/rB0QQlfa7f4&hl=en&fs=1&"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="http://www.youtube.com/v/rB0QQlfa7f4&hl=en&fs=1&" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="560" height="340"></embed></object></html><br />
<br />
<br />
''Common characteristics of chemotaxis receptors:'' All 6 receptors we identified couple to the [http://en.wikipedia.org/wiki/Gi_alpha_subunit Gi] signaling pathway. The behavior of the M3/M2 chimera, however, suggests that it may be possible to convert receptors with different coupling specificities into chemotaxis receptors. To generate this chimera, the third intracellular loop (i3) from the M3 muscarinic acetylcholine receptor ([http://en.wikipedia.org/wiki/Gq_alpha_subunit Gq] coupled) was exchanged with that of M2 muscarinic receptor (Gi coupled). It has previously been shown that this chimera now couples to Gi.<br />
<br />
[[Image:chimera.png|350px|thumb|center|i3 loop determines the coupling specificty of the M3 muscarinic acetylcholine receptor.]]<br />
<br />
<br />
Why the i3 loop allows the M3/M2 chimeric receptor to signal to the cell's chemotaxis machinery remains a question for further study. However, the possibility exists that more Gq-coupled (and possibly [http://en.wikipedia.org/wiki/Gs_alpha_subunit Gs]-coupled) receptors could be converted in this way, thus dramatically increasing the number of potential chemotaxis targets.<br />
<br />
<br />
<br />
== '''Part 2: Navigation: Tuning receptor sensitivity'''==<br />
<br />
=== Motivation: ''Why is this useful?'' ===<br />
In our experiments, we could control exactly how much ligand was presented to our cells. In "real-life," however, we would want our cellular robots to be able to respond to a variety of signal strengths: from very low to very high. To accomplish this, we would want to be able to control the ''sensitivity'' of our receptors, or how the receptor's output changes when the measured quantity of ligand changes.<br />
<br />
=== Approach: ===<br />
We felt that one key determinant of sensitivity would be the number of receptors present at the plasma membrane of the cell. Therefore, we measured the migration response of a receptor (delta Opioid receptor) whose recycling behavior could be engineered by fusing different recycling interaction modules to the C terminus of the GPCR.<br />
<br />
== '''Part 3: SPEED: Engineering accelerators and brakes'''== <br />
'''A cellular cruise control by modulating cell polarity with feedback loops''' <br />
<br />
=== Motivation: ''why is this useful?'' ===<br />
Just like we have control over speed in a car – we can brake or accelerate – it would be useful to engineer such behavior into our cellular nanorobots.<br />
Just think about it: We could '''speed cells up''' so that they reach their targets faster and '''stop them''' once they have arrived or do not behave properly.<br />
<br />
=== Background: ===<br />
For these experiments we chose ''Dictyostelium discoideum'' cells to test our prototypical brakes and accelerators quickly. We expect that our brakes and accelerators can be used in a plug and play fashion because Dicty’s way of movement is very similar to a neutrophil’s:<br />
<br />
When a receptor binds chemoattractant, it induces the conversion of PhosphatidylInositol(4,5)bisphosphate (PIP2) to PhosphatidylInositol(3,4,5)trisphosphate (PIP3) (two signaling lipids in the plasma membrane) at the front of our cells. In a '''positive feedback loop''' PIP3 triggers the formation of more PIP3 at the front while similarly PIP2 leads to more PIP2 production at the sides and rear of the cell. This system sets the axis of polarity of the cell. The PIP3 patch at the front functions as a ‘turbo boost’ pushing the cell forward.<br />
<br />
<br />
[[Image:BACKGROUND1.jpg|500px|thumb|center|'''Polarized distribution of PIP3 and PIP2:''' A patch of PIP3 is localized at the front of a cell while PIP2 is at the back. Feedback loops are used to establish and maintain this polarized distribution. ]]<br />
<br />
=== Approach: ===<br />
Inspired by nature we tried to build accelerators and brakes by introducing our own synthetic protein based feedback loops. We designed feedback elements by fusing localization and catalytic domains involved in PIP3 production and degradation to modulate localization and level of PIP3 and PIP2 in the cell. <br />
<br />
Here is an example of a positive feedback loop: a PIP3 binding localization domain fused to a PIP3 producing catalytic domain could produce more PIP3 where there is already PIP3- at the front. This might strengthen polarity and accelerate a cell.<br />
<br />
[[Image:positive_feedback_loop2.jpg|500px|thumb|center|'''Synthetic protein based feedback loops:''' One example: a positive feedback loop comprising a PIP3 binding domain fused to a PIP3 generating enzyme.]]<br />
<br />
=== Results: ===<br />
Over the summer we assembled more than 100 fusions of localization and catalytic domains and screened whether they work. How? We measured the effect our constructs have on motility of ''Dictyostelium'' cells: stronger polarity should make cells faster while weaker polarity ought to slow them down!<br />
<br />
[[Matrix of Fusion Constructs|Here]] is an overview of all feedback loops we screened and the effect they had on the speed of cells. <br />
We used automated cell tracking on more than 196 hours worth of movies (note: one movie is 10 minutes!) and identified strains that moved faster or slower at a very stringent statistical cutoff (p<0.0001). <br />
<br />
<br />
This way we were able to identify '''7 brakes and 1 accelerator'''!<br />
<br />
Check out the movie of '''one of our strong brakes (PTEN-RasC dominant active (da))[http://www.youtube.com/watch?v=_9od33Nx06Y] compared to wildtype [http://www.youtube.com/watch?v=Vtdtf8-zSRs].''' <br />
<br />
<br />
[[Image:HO230.jpg|450px|thumb|right|'''PTEN fused to RasC da (PIP2 binding - PIP3 generating); speed: 3.5 um/min''' Cells were plated in buffer and basic motile behavior was recorded for 10 minutes taking 1 picture every 15 seconds.]] [[Image:wt.jpg|450px|thumb|left|'''wildtype; speed: 5.9 um/min'''<br />
Cells were plated in buffer and basic motile behavior was recorded for 10 minutes taking 1 picture every 15 seconds.]]<br />
<br />
<br />
We hypothesize that this construct acts as a '''negative feedback loop on PIP2''' - (generating PIP3 where PIP2 should be) thereby confusing the cell with multiple fronts:<br />
<br />
[[Image:Brake2.jpg|900px|]]<br />
<br />
This is indeed an effect of fusing the particular localization to the catalytic domain as neither of them alone has such a strong effect ([[ see details]]).<br />
<br />
=== Summary and outlook: ===<br />
We have screened more than 100 synthetic feedback elements for their ability to accelerate or slow down speed of cell motility. We have isolated a hand full of functional elements. Now we need to confirm the mechanism of action of these elements. In the future we would like to make them inducible by a signal from outside – like a stoplight!<br />
<br />
=Methods=<br />
<br />
*[[Media:Aar1_shuffle.pdf|Cloning: Aar1 Shuffle]] - besides BBFRFC28, here is an actual step by step scheme we used for some of our combinatorial assembly of parts<br />
*[[Media:DICTY TRANSFORMATION.pdf|Dicty: Transformation Protocol]] - here is how we get our constructs into Dicty<br />
*[[Media:DICTY-analyzing_motility.pdf|Dicty: Motility Assay Protocol]] - here is how we prepare and film Dicty cells<br />
<br />
=Selected Reading=</div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T20:44:59Z<p>EthanChan: /* Team Members */</p>
<hr />
<div>{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="3"|<br />
<br />
== '''Team Members''' ==<br />
<br />
{| width=100% align="center" border="0" cellpading="20' cellspacing="20"<br />
|'''Students:'''<br />
<br />
*'''High School Student''': [[Allen Cai]]<br />
*'''High School Student''': [[Alex Smith]]<br />
*'''High School Student''': [[Edna Miao]]<br />
*'''High School Student''': [[Ethan Chan]]<br />
*'''High School Student''': [[Eric Wong]]<br />
*'''High School Student''': [[Jackie Tam]]<br />
*'''High School Student''': [[Ryan Liang]]<br />
<br />
<br />
*'''Undergrad Student''': [[Cathy Liu]]<br />
*'''Undergrad Student''': [[Ryan Quan]]<br />
<br />
<br />
*'''International Undergrad Student''': [[Hansi Liu]]<br />
*'''International Undergrad Student''': [[Katja Kolar]]<br />
<br />
<br />
|'''Buddies:'''<br />
<br />
*''' Advisor''': [[Wendell Lim]]<br />
*''' Advisor''': [[Orion Weiner]]<br />
*''' Advisor''': [[James Onuffer]]<br />
*''' Scientific Director''': [[Ben Rhau]]<br />
*''' Scientific Director''': [[Oliver Hoeller]]<br />
*''' Education Development''': [[Raquel Gomes]]<br />
*''' Buddy''': [[Aynur Tasdemir]]<br />
*''' Buddy''': [[Jason Park]] <br />
*''' Buddy''': [[Andrew Houk]] <br />
*''' Buddy''': [[Delquin Gong]] <br />
*''' Buddy''': [[Arthur Millius]] <br />
*''' Buddy''': [[David Pincus]]<br />
*''' Buddy''': [[Bethany Simmons]]<br />
*''' Buddy''': [[Saber Khan]]<br />
|}<br />
<br />
<gallery><br />
Buddies<br />
Image:Wendell.JPG|Wendell Lim<br />
Image:Orion_weiner.gif|Orion Weiner<br />
Image:.|James Onuffer<br />
<br />
Image:.|Raquel Gomes<br />
<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery><br />
<br />
<gallery><br />
Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery></div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T20:42:46Z<p>EthanChan: /* Team Members */</p>
<hr />
<div>{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="3"|<br />
<br />
== '''Team Members''' ==<br />
<br />
{| width=100% align="center" border="0" cellpading="20' cellspacing="20"<br />
|'''Students:'''<br />
<br />
*'''High School Student''': [[Allen Cai]]<br />
*'''High School Student''': [[Alex Smith]]<br />
*'''High School Student''': [[Edna Miao]]<br />
*'''High School Student''': [[Ethan Chan]]<br />
*'''High School Student''': [[Eric Wong]]<br />
*'''High School Student''': [[Jackie Tam]]<br />
*'''High School Student''': [[Ryan Liang]]<br />
<br />
<br />
*'''Undergrad Student''': [[Cathy Liu]]<br />
*'''Undergrad Student''': [[Ryan Quan]]<br />
<br />
<br />
*'''International Undergrad Student''': [[Hansi Liu]]<br />
*'''International Undergrad Student''': [[Katja Kolar]]<br />
<br />
<br />
|'''Buddies:'''<br />
<br />
*''' Advisor''': [[Wendell Lim]]<br />
*''' Advisor''': [[Orion Weiner]]<br />
*''' Advisor''': [[James Onuffer]]<br />
*''' Scientific Director''': [[Ben Rhau]]<br />
*''' Scientific Director''': [[Oliver Hoeller]]<br />
*''' Education Development''': [[Raquel Gomes]]<br />
*''' Buddy''': [[Aynur Tasdemir]]<br />
*''' Buddy''': [[Jason Park]] <br />
*''' Buddy''': [[Andrew Houk]] <br />
*''' Buddy''': [[Delquin Gong]] <br />
*''' Buddy''': [[Arthur Millius]] <br />
*''' Buddy''': [[David Pincus]]<br />
*''' Buddy''': [[Bethany Simmons]]<br />
*''' Buddy''': [[Saber Khan]]<br />
|}<br />
<br />
<gallery><br />
Advisors<br />
Image:Wendell.JPG|Wendell Lim<br />
Image:Orion_weiner.gif|Orion Weiner<br />
Image:.|James Onuffer<br />
</gallery><br />
<br />
<gallery><br />
Educational Director<br />
Image:.|Raquel Gomes<br />
</gallery><br />
<br />
<gallery><br />
Scientific Directors<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
</gallery><br />
<br />
<gallery><br />
Buddies<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery><br />
<br />
<gallery><br />
High School Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
</gallery><br />
|}<br />
<br />
<gallery><br />
Under Grads<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery></div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T20:42:30Z<p>EthanChan: /* Team Members */</p>
<hr />
<div>{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="3"|<br />
<br />
== '''Team Members''' ==<br />
<br />
{| width=100% align="center" border="0" cellpading="20' cellspacing="20"<br />
|'''Students:'''<br />
<br />
*'''High School Student''': [[Allen Cai]]<br />
*'''High School Student''': [[Alex Smith]]<br />
*'''High School Student''': [[Edna Miao]]<br />
*'''High School Student''': [[Ethan Chan]]<br />
*'''High School Student''': [[Eric Wong]]<br />
*'''High School Student''': [[Jackie Tam]]<br />
*'''High School Student''': [[Ryan Liang]]<br />
<br />
<br />
*'''Undergrad Student''': [[Cathy Liu]]<br />
*'''Undergrad Student''': [[Ryan Quan]]<br />
<br />
<br />
'''International Undergrad:'''<br />
*'''International Undergrad Student''': [[Hansi Liu]]<br />
*'''International Undergrad Student''': [[Katja Kolar]]<br />
<br />
<br />
|'''Buddies:'''<br />
<br />
*''' Advisor''': [[Wendell Lim]]<br />
*''' Advisor''': [[Orion Weiner]]<br />
*''' Advisor''': [[James Onuffer]]<br />
*''' Scientific Director''': [[Ben Rhau]]<br />
*''' Scientific Director''': [[Oliver Hoeller]]<br />
*''' Education Development''': [[Raquel Gomes]]<br />
*''' Buddy''': [[Aynur Tasdemir]]<br />
*''' Buddy''': [[Jason Park]] <br />
*''' Buddy''': [[Andrew Houk]] <br />
*''' Buddy''': [[Delquin Gong]] <br />
*''' Buddy''': [[Arthur Millius]] <br />
*''' Buddy''': [[David Pincus]]<br />
*''' Buddy''': [[Bethany Simmons]]<br />
*''' Buddy''': [[Saber Khan]]<br />
|}<br />
<br />
<gallery><br />
Advisors<br />
Image:Wendell.JPG|Wendell Lim<br />
Image:Orion_weiner.gif|Orion Weiner<br />
Image:.|James Onuffer<br />
</gallery><br />
<br />
<gallery><br />
Educational Director<br />
Image:.|Raquel Gomes<br />
</gallery><br />
<br />
<gallery><br />
Scientific Directors<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
</gallery><br />
<br />
<gallery><br />
Buddies<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery><br />
<br />
<gallery><br />
High School Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
</gallery><br />
|}<br />
<br />
<gallery><br />
Under Grads<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery></div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T20:33:20Z<p>EthanChan: /* Where we're from */</p>
<hr />
<div>{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="3"|<br />
<br />
== '''Team Members''' ==<br />
<br />
{| width=100% align="center" border="0" cellpading="20' cellspacing="20"<br />
|'''High School students:'''<br />
<br />
*'''Student''': [[Allen Cai]]<br />
*'''Student''': [[Alex Smith]]<br />
*'''Student''': [[Edna Miao]]<br />
*'''Student''': [[Ethan Chan]]<br />
*'''Student''': [[Eric Wong]]<br />
*'''Student''': [[Jackie Tam]]<br />
*'''Student''': [[Ryan Liang]]<br />
<br />
<br />
<br />
'''Undergrads:'''<br />
<br />
*'''Student''': [[Cathy Liu]]<br />
*'''Student''': [[Ryan Quan]]<br />
<br />
<br />
'''International Undergrad:'''<br />
*'''Student''': [[Hansi Liu]]<br />
*'''Student''': [[Katja Kolar]]<br />
<br />
<br />
|'''Buddies:'''<br />
<br />
*''' Advisor''': [[Wendell Lim]]<br />
*''' Advisor''': [[Orion Weiner]]<br />
*''' Advisor''': [[James Onuffer]]<br />
*''' Scientific Director''': [[Ben Rhau]]<br />
*''' Scientific Director''': [[Oliver Hoeller]]<br />
*''' Education Development''': [[Raquel Gomes]]<br />
*''' Buddy''': [[Aynur Tasdemir]]<br />
*''' Buddy''': [[Jason Park]] <br />
*''' Buddy''': [[Andrew Houk]] <br />
*''' Buddy''': [[Delquin Gong]] <br />
*''' Buddy''': [[Arthur Millius]] <br />
*''' Buddy''': [[David Pincus]]<br />
*''' Buddy''': [[Bethany Simmons]]<br />
*''' Buddy''': [[Saber Khan]]<br />
|}<br />
<br />
<gallery><br />
Advisors<br />
Image:Wendell.JPG|Wendell Lim<br />
Image:Orion_weiner.gif|Orion Weiner<br />
Image:.|James Onuffer<br />
</gallery><br />
<br />
<gallery><br />
Educational Director<br />
Image:.|Raquel Gomes<br />
</gallery><br />
<br />
<gallery><br />
Scientific Directors<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
</gallery><br />
<br />
<gallery><br />
Buddies<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery><br />
<br />
<gallery><br />
High School Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
</gallery><br />
|}<br />
<br />
<gallery><br />
Under Grads<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery></div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T20:32:41Z<p>EthanChan: /* What we did */</p>
<hr />
<div>{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="3"|<br />
<br />
== '''Team Members''' ==<br />
<br />
{| width=100% align="center" border="0" cellpading="20' cellspacing="20"<br />
|'''High School students:'''<br />
<br />
*'''Student''': [[Allen Cai]]<br />
*'''Student''': [[Alex Smith]]<br />
*'''Student''': [[Edna Miao]]<br />
*'''Student''': [[Ethan Chan]]<br />
*'''Student''': [[Eric Wong]]<br />
*'''Student''': [[Jackie Tam]]<br />
*'''Student''': [[Ryan Liang]]<br />
<br />
<br />
<br />
'''Undergrads:'''<br />
<br />
*'''Student''': [[Cathy Liu]]<br />
*'''Student''': [[Ryan Quan]]<br />
<br />
<br />
'''International Undergrad:'''<br />
*'''Student''': [[Hansi Liu]]<br />
*'''Student''': [[Katja Kolar]]<br />
<br />
<br />
|'''Buddies:'''<br />
<br />
*''' Advisor''': [[Wendell Lim]]<br />
*''' Advisor''': [[Orion Weiner]]<br />
*''' Advisor''': [[James Onuffer]]<br />
*''' Scientific Director''': [[Ben Rhau]]<br />
*''' Scientific Director''': [[Oliver Hoeller]]<br />
*''' Education Development''': [[Raquel Gomes]]<br />
*''' Buddy''': [[Aynur Tasdemir]]<br />
*''' Buddy''': [[Jason Park]] <br />
*''' Buddy''': [[Andrew Houk]] <br />
*''' Buddy''': [[Delquin Gong]] <br />
*''' Buddy''': [[Arthur Millius]] <br />
*''' Buddy''': [[David Pincus]]<br />
*''' Buddy''': [[Bethany Simmons]]<br />
*''' Buddy''': [[Saber Khan]]<br />
|}<br />
<br />
<gallery><br />
Advisors<br />
Image:Wendell.JPG|Wendell Lim<br />
Image:Orion_weiner.gif|Orion Weiner<br />
Image:.|James Onuffer<br />
</gallery><br />
<br />
<gallery><br />
Educational Director<br />
Image:.|Raquel Gomes<br />
</gallery><br />
<br />
<gallery><br />
Scientific Directors<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
</gallery><br />
<br />
<gallery><br />
Buddies<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery><br />
<br />
<gallery><br />
High School Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
</gallery><br />
|}<br />
<br />
<gallery><br />
Under Grads<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery><br />
<br />
<br />
<br />
== '''Where we're from''' ==<br />
<br />
Extra pages:<br />
[[subteam1]]</div>EthanChanhttp://2009.igem.org/David_PincusDavid Pincus2009-10-20T20:27:20Z<p>EthanChan: New page: ==Rockstar==</p>
<hr />
<div>==Rockstar==</div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T20:26:42Z<p>EthanChan: /* Team Members */</p>
<hr />
<div>{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="3"|<br />
<br />
== '''Team Members''' ==<br />
<br />
{| width=100% align="center" border="0" cellpading="20' cellspacing="20"<br />
|'''High School students:'''<br />
<br />
*'''Student''': [[Allen Cai]]<br />
*'''Student''': [[Alex Smith]]<br />
*'''Student''': [[Edna Miao]]<br />
*'''Student''': [[Ethan Chan]]<br />
*'''Student''': [[Eric Wong]]<br />
*'''Student''': [[Jackie Tam]]<br />
*'''Student''': [[Ryan Liang]]<br />
<br />
<br />
<br />
'''Undergrads:'''<br />
<br />
*'''Student''': [[Cathy Liu]]<br />
*'''Student''': [[Ryan Quan]]<br />
<br />
<br />
'''International Undergrad:'''<br />
*'''Student''': [[Hansi Liu]]<br />
*'''Student''': [[Katja Kolar]]<br />
<br />
<br />
|'''Buddies:'''<br />
<br />
*''' Advisor''': [[Wendell Lim]]<br />
*''' Advisor''': [[Orion Weiner]]<br />
*''' Advisor''': [[James Onuffer]]<br />
*''' Scientific Director''': [[Ben Rhau]]<br />
*''' Scientific Director''': [[Oliver Hoeller]]<br />
*''' Education Development''': [[Raquel Gomes]]<br />
*''' Buddy''': [[Aynur Tasdemir]]<br />
*''' Buddy''': [[Jason Park]] <br />
*''' Buddy''': [[Andrew Houk]] <br />
*''' Buddy''': [[Delquin Gong]] <br />
*''' Buddy''': [[Arthur Millius]] <br />
*''' Buddy''': [[David Pincus]]<br />
*''' Buddy''': [[Bethany Simmons]]<br />
*''' Buddy''': [[Saber Khan]]<br />
|}<br />
<br />
<gallery><br />
Advisors<br />
Image:Wendell.JPG|Wendell Lim<br />
Image:Orion_weiner.gif|Orion Weiner<br />
Image:.|James Onuffer<br />
</gallery><br />
<br />
<gallery><br />
Educational Director<br />
Image:.|Raquel Gomes<br />
</gallery><br />
<br />
<gallery><br />
Scientific Directors<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
</gallery><br />
<br />
<gallery><br />
Buddies<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery><br />
<br />
<gallery><br />
High School Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
</gallery><br />
|}<br />
<br />
<gallery><br />
Under Grads<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery><br />
<br />
== '''What we did''' ==<br />
<br />
(Provide proper attribution for all work)<br />
<br />
<br />
== '''Where we're from''' ==<br />
<br />
Extra pages:<br />
[[subteam1]]</div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T20:11:01Z<p>EthanChan: </p>
<hr />
<div>{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="3"|<br />
<br />
== '''Team Members''' ==<br />
<br />
{| width=100% align="center" border="0" cellpading="20' cellspacing="20"<br />
|'''High School students:'''<br />
<br />
*'''Student''': [[Allen Cai]]<br />
*'''Student''': [[Alex Smith]]<br />
*'''Student''': [[Edna Miao]]<br />
*'''Student''': [[Ethan Chan]]<br />
*'''Student''': [[Eric Wong]]<br />
*'''Student''': [[Jackie Tam]]<br />
*'''Student''': [[Ryan Liang]]<br />
<br />
<br />
<br />
'''Undergrads:'''<br />
<br />
*'''Student''': [[Cathy Liu]]<br />
*'''Student''': [[Ryan Quan]]<br />
<br />
<br />
'''International Undergrad:'''<br />
*'''Student''': [[Hansi Liu]]<br />
*'''Student''': [[Katja Kolar]]<br />
<br />
<br />
|'''Buddies:'''<br />
<br />
*''' Advisor''': [[Wendell Lim]]<br />
*''' Advisor''': [[Orion Weiner]]<br />
*''' Advisor''': [[James Onuffer]]<br />
*''' Scientific Director''': [[Ben Rhau]]<br />
*''' Scientific Director''': [[Oliver Hoeller]]<br />
*''' Education Development''': [[Raquel Gomes]]<br />
*''' Buddy''': [[Aynur Tasdemir]]<br />
*''' Buddy''': [[Jason Park]] <br />
*''' Buddy''': [[Andrew Houk]] <br />
*''' Buddy''': [[Delquin Gong]] <br />
*''' Buddy''': [[Arthur Millius]] <br />
*''' Buddy''': [[UCSF/David_Pincus|David Pincus]]<br />
*''' Buddy''': [[Bethany Simmons]]<br />
*''' Buddy''': [[Saber Khan]]<br />
|}<br />
<br />
<gallery><br />
Advisors<br />
Image:Wendell.JPG|Wendell Lim<br />
Image:Orion_weiner.gif|Orion Weiner<br />
Image:.|James Onuffer<br />
</gallery><br />
<br />
<gallery><br />
Educational Director<br />
Image:.|Raquel Gomes<br />
</gallery><br />
<br />
<gallery><br />
Scientific Directors<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
</gallery><br />
<br />
<gallery><br />
Buddies<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery><br />
<br />
<gallery><br />
High School Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
</gallery><br />
|}<br />
<br />
<gallery><br />
Under Grads<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery><br />
<br />
<br />
<br />
== '''What we did''' ==<br />
<br />
(Provide proper attribution for all work)<br />
<br />
<br />
== '''Where we're from''' ==<br />
<br />
Extra pages:<br />
[[subteam1]]</div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T19:48:55Z<p>EthanChan: /* Introduction to the 2009 UCSF IGEM Team */</p>
<hr />
<div>{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="3"|<br />
<br />
'''Advisors:'''<br />
*'''[[Wendell Lim]]'''<br />
*'''Orion Weiner''' <br />
*'''James Onuffer'''<br />
<br />
'''Educational Director'''<br />
*'''[[Raquel Gomes]]''' <br />
<br />
'''Scientific Directors'''<br />
*'''[[Ben Rhau]]''' <br />
*'''[[Oliver Hoeller]]''' <br />
<br />
'''Buddies:'''<br />
*'''[[Aynur Tasdemir]]''' <br />
*'''[[Jason Park]]''' <br />
*'''[[Andrew Houk]]''' <br />
*'''[[Delquin Gong]]''' <br />
*'''[[Arthur Millius]]''' <br />
*'''[[Bethany Simmons]]'''<br />
<br />
*'''[[Saber Khan]]''' <br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''High School Students:'''<br />
<br />
*'''[[Allen Cai]]''' <br />
*'''[[Alex Smith]]''' <br />
*'''[[Edna Miao]]''' <br />
*'''[[Ethan Chan]]''' <br />
*'''[[Eric Wong]]''' <br />
*'''[[Jackie Tam]]''' <br />
*'''[[Ryan Liang]]''' <br />
<br />
<br />
<br />
'''Undergrads:'''<br />
*'''[[Cathy Liu]]'''<br />
*'''[[Ryan Quan]]'''<br />
*'''[[Hansi Liu]]'''<br />
*'''[[Katja Kolar]]'''<br />
|<br />
<gallery><br />
Advisors<br />
Image:Wendell.JPG|Wendell Lim<br />
Image:Orion_weiner.gif|Orion Weiner<br />
Image:.|James Onuffer<br />
</gallery><br />
<br />
<gallery><br />
Educational Director<br />
Image:.|Raquel Gomes<br />
</gallery><br />
<br />
<gallery><br />
Scientific Directors<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
</gallery><br />
<br />
<gallery><br />
Buddies<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery><br />
<br />
<gallery><br />
High School Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
</gallery><br />
|}<br />
<br />
<gallery><br />
Under Grads<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery><br />
<br />
== '''What we did''' ==<br />
<br />
(Provide proper attribution for all work)<br />
<br />
<br />
== '''Where we're from''' ==<br />
<br />
Extra pages:<br />
[[subteam1]]</div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T19:31:18Z<p>EthanChan: /* Introduction to the 2009 UCSF IGEM Team */</p>
<hr />
<div>{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="1"|<br />
<br />
'''Advisors:'''<br />
*'''[[Wendell Lim]]'''<br />
*'''Orion Weiner''' <br />
*'''James Onuffer'''<br />
<br />
'''Educational Director'''<br />
*'''[[Raquel Gomes]]''' <br />
<br />
'''Scientific Directors'''<br />
*'''[[Ben Rhau]]''' <br />
*'''[[Oliver Hoeller]]''' <br />
<br />
'''Buddies:'''<br />
*'''[[Aynur Tasdemir]]''' <br />
*'''[[Jason Park]]''' <br />
*'''[[Andrew Houk]]''' <br />
*'''[[Delquin Gong]]''' <br />
*'''[[Arthur Millius]]''' <br />
*'''[[Bethany Simmons]]'''<br />
<br />
*'''[[Saber Khan]]''' <br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''High School Students:'''<br />
<br />
*'''[[Allen Cai]]''' <br />
*'''[[Alex Smith]]''' <br />
*'''[[Edna Miao]]''' <br />
*'''[[Ethan Chan]]''' <br />
*'''[[Eric Wong]]''' <br />
*'''[[Jackie Tam]]''' <br />
*'''[[Ryan Liang]]''' <br />
<br />
<br />
<br />
'''Undergrads:'''<br />
*'''[[Cathy Liu]]'''<br />
*'''[[Ryan Quan]]'''<br />
*'''[[Hansi Liu]]'''<br />
*'''[[Katja Kolar]]'''<br />
|<br />
<gallery><br />
Advisors<br />
Image:Wendell.JPG|Wendell Lim<br />
Image:Orion_weiner.gif|Orion Weiner<br />
Image:.|center| James Onuffer<br />
</gallery><br />
<br />
<gallery><br />
Educational Director<br />
Image:.|Raquel Gomes<br />
</gallery><br />
<br />
<gallery><br />
Scientific Directors<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
</gallery><br />
<br />
<gallery><br />
Buddies<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery><br />
<br />
<gallery><br />
High School Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
</gallery><br />
|}<br />
<br />
<gallery><br />
Under Grads<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery><br />
<br />
== '''What we did''' ==<br />
<br />
(Provide proper attribution for all work)<br />
<br />
<br />
== '''Where we're from''' ==<br />
<br />
Extra pages:<br />
[[subteam1]]</div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T19:26:54Z<p>EthanChan: </p>
<hr />
<div>{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="1"|<br />
<br />
'''Advisors:'''<br />
*'''[[Wendell Lim]]'''<br />
*'''Orion Weiner''' <br />
*'''James Onuffer'''<br />
<br />
'''Educational Director'''<br />
*'''[[Raquel Gomes]]''' <br />
<br />
'''Scientific Directors'''<br />
*'''[[Ben Rhau]]''' <br />
*'''[[Oliver Hoeller]]''' <br />
<br />
'''Buddies:'''<br />
*'''[[Aynur Tasdemir]]''' <br />
*'''[[Jason Park]]''' <br />
*'''[[Andrew Houk]]''' <br />
*'''[[Delquin Gong]]''' <br />
*'''[[Arthur Millius]]''' <br />
*'''[[Bethany Simmons]]'''<br />
<br />
*'''[[Saber Khan]]''' <br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''High School Students:'''<br />
<br />
*'''[[Allen Cai]]''' <br />
*'''[[Alex Smith]]''' <br />
*'''[[Edna Miao]]''' <br />
*'''[[Ethan Chan]]''' <br />
*'''[[Eric Wong]]''' <br />
*'''[[Jackie Tam]]''' <br />
*'''[[Ryan Liang]]''' <br />
<br />
<br />
<br />
'''Undergrads:'''<br />
*'''[[Cathy Liu]]'''<br />
*'''[[Ryan Quan]]'''<br />
*'''[[Hansi Liu]]'''<br />
*'''[[Katja Kolar]]'''<br />
|<br />
<gallery><br />
Advisors<br />
Image:Wendell.JPG|Wendell Lim<br />
Image:Orion_weiner.gif|Orion Weiner<br />
Image:.|James Onuffer<br />
</gallery><br />
<br />
<gallery><br />
Educational Director<br />
Image:.|Raquel Gomes<br />
</gallery><br />
<br />
<gallery><br />
Scientific Directors<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
</gallery><br />
<br />
<gallery><br />
Buddies<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery><br />
<br />
<gallery><br />
High School Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
</gallery><br />
|}<br />
<br />
<gallery><br />
Under Grads<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery><br />
<br />
== '''What we did''' ==<br />
<br />
(Provide proper attribution for all work)<br />
<br />
<br />
== '''Where we're from''' ==<br />
<br />
Extra pages:<br />
[[subteam1]]</div>EthanChanhttp://2009.igem.org/Wendell_LimWendell Lim2009-10-20T19:21:28Z<p>EthanChan: New page: I have had a lab at UCSF for 13 years The most rewarding thing is working with bright, open-minded young scientists and seeing them develop. Its great to see that most of the iGEM kids th...</p>
<hr />
<div>I have had a lab at UCSF for 13 years<br />
<br />
The most rewarding thing is working with bright, open-minded young scientists and seeing them develop. Its great to see that most of the iGEM kids that we have worked with have continued to be excited about science and synthetic biology.</div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T19:21:20Z<p>EthanChan: /* Introduction to the 2009 UCSF IGEM Team */</p>
<hr />
<div><br />
<br />
<br />
{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="1"|<br />
<br />
'''Advisors:'''<br />
*'''[[Wendell Lim]]'''<br />
*'''Orion Weiner''' <br />
*'''James Onuffer'''<br />
<br />
'''Educational Director'''<br />
*'''[[Raquel Gomes]]''' <br />
<br />
'''Scientific Directors'''<br />
*'''[[Ben Rhau]]''' <br />
*'''[[Oliver Hoeller]]''' <br />
<br />
'''Buddies:'''<br />
*'''[[Aynur Tasdemir]]''' <br />
*'''[[Jason Park]]''' <br />
*'''[[Andrew Houk]]''' <br />
*'''[[Delquin Gong]]''' <br />
*'''[[Arthur Millius]]''' <br />
*'''[[Bethany Simmons]]'''<br />
<br />
*'''[[Saber Khan]]''' <br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''High School Students:'''<br />
<br />
*'''[[Allen Cai]]''' <br />
*'''[[Alex Smith]]''' <br />
*'''[[Edna Miao]]''' <br />
*'''[[Ethan Chan]]''' <br />
*'''[[Eric Wong]]''' <br />
*'''[[Jackie Tam]]''' <br />
*'''[[Ryan Liang]]''' <br />
<br />
<br />
<br />
'''Undergrads:'''<br />
*'''[[Cathy Liu]]'''<br />
*'''[[Ryan Quan]]'''<br />
*'''[[Hansi Liu]]'''<br />
*'''[[Katja Kolar]]'''<br />
|<br />
<gallery><br />
Advisors<br />
Image:Wendell.JPG|Wendell Lim<br />
Image:Orion_weiner.gif|Orion Weiner<br />
Image:.|James Onuffer<br />
</gallery><br />
<br />
<gallery><br />
Educational Director<br />
Image:.|Raquel Gomes<br />
</gallery><br />
<br />
<gallery><br />
Scientific Directors<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
</gallery><br />
<br />
<gallery><br />
Buddies<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery><br />
<br />
<gallery><br />
High School Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
</gallery><br />
|}<br />
<br />
<gallery><br />
Under Grads<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery><br />
<br />
== '''What we did''' ==<br />
<br />
(Provide proper attribution for all work)<br />
<br />
<br />
== '''Where we're from''' ==<br />
<br />
Extra pages:<br />
[[subteam1]]</div>EthanChanhttp://2009.igem.org/Team:UCSF/NotebookTeam:UCSF/Notebook2009-10-20T16:31:36Z<p>EthanChan: /* HL60 Group */</p>
<hr />
<div>== '''HL60 Group''' ==<br />
* '''Goals:''' Our goals for this project are to screen 30 various different GPCR's (G couple protein receptors)to determine which receptors mediate chemotaxis using the high through put transwell assay. After first phase screening using 5-6 day differentiated HL-60 cell transfected with known receptors is completed, we move on to the secondary stage of screening with viable chemotatic receptors. During the second stage of screening we fused "Actin Modulators", "PIP3 Modulators", and "GEF Activators" in hopes of an accelerated chemotatic response.<br />
*'''''Engineering Navigation:''''' <br />
*''sending cells to new targets - ''GPCR Screening<br />
<br />
*''tuning receptor sensitivity - ''Forced localization of effector proteins to GPCRs<br />
*'''''Payload:''''' <br />
*''can we make our cells carry stuff? - ''attach beads to cells<br />
><br />
*[[Cathy Liu's notebook]]: I was one of the main players on team HL-60 who conducted the transwells for the GPCR screen. This included transfections and analysis of data. I was Aynur’s student and Ryan’s and Eric’s buddy. And what a beautiful team we made!<br />
*[[Eric Wong's notebook]]: I worked on cloning, PCR, Gel purification and extraction, and was apart of the GPCR screening team.<br />
*[[Jackie Tam's notebook]]: I was responsible for cloning, microscopy, and video analysis.<br />
*[[Ryan Liang's notebook]]:I cloned parts to be used in the GPCR screens, transfected HL-60 cells with various different GPCRs, ran transwell assays, and analyzed/compared transwell assay results.<br />
*[[Katja Kolar's notebook]]: I did microscopy with wt and hM4D-transfected HL-60 cells, experiments for the "Payload" part of the project, and cloned HL-60 team constructs.<br />
*[[Hansi Liu's notebook]]:<br />
<br />
== '''Dictyostelium Group''' ==<br />
* '''Goals:''' <br />
* '''''Engineering brakes and accelerators:''''' <br />
* ''modulating PIP3 polarity by building synthetic protein based feedback loops''<br />
* workflow:<br />
*1. PCR parts: catalytic and localization domains<br />
*2. Create combinations of localization domains and catalytic domains <br />
*3. Generate Dictyostelium strains expressing feedback elements<br />
*4. Measure motility parameters (speed, directionality)<br />
*3. Analyze data. Create histograms and compare if our plasmid had any effect to dicty.Do they cause them to move faster or slower?<br />
*<br />
<br />
><br />
*[[Allen Cai's notebook]]: I worked mostly on the clonning part of the project. I ligated a lot of the localization and catalytic domains together. I also took care so some dicty strains and made some movies of those dicty strains.<br />
*[[Alex Smith's notebook]]:<br />
*[[Edna Miao's notebook]]: I transformed new constructs into Dicty, analyzed data, and worked with wildtype and PTEN null dicty.<br />
*[[Ethan Chan's notebook]]: Constructed Dicty cells with new parts, analyzed data, and worked with wildtype and PTEN null dicty cells. <br />
*[[Ryan Quan's notebook]]:I worked on team dicty and taught the students some basic cloning techniques but mainly learned many new things along with them.<br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}</div>EthanChanhttp://2009.igem.org/Team:UCSF/Our_summer_experienceTeam:UCSF/Our summer experience2009-10-20T16:30:52Z<p>EthanChan: /* Personal Blogs about the Experience */</p>
<hr />
<div>== '''Summer Curriculum''' ==<br />
* '''[[Selecting/Development of the iGEM team]]''':High School teacher Mr.Cachianes hand selected seven students from his second year biotechnology class for the iGEM team.<br />
* '''[[Pre-meetings]]''':Starting early April, Mr.Cachianes and his seven students met with UCSF's iGEM coordinators to start the pre-project and learn more about synthetic biology and cell motility.<br />
* '''[[Bootcamp]]''': For two weeks, the iGEM team met everyday for four hour lectures on the mechanisms of cell motility, and discussed scientific papers.<br />
* '''[[Student Brainstorming]]''':Bootcamp culminated in one large brainstorming project where we put our heads together and put what we learned to the test by trying to find ways to brake, accelerate, and steer a cell. <br />
* '''[[Execution]]''':We took the best ideas from brainstorming, modified them, and prepared to carry them out.<br />
*'''[[Curriculum for Teachers]]''' by Saber Khan: Cells as Robots Unit: iGEM/UCSF Summer 2009<br />
<br />
== '''Personal Blogs about the Experience''' ==<br />
* [http://arsigem.blogspot.com/ '''Alex Smith''']<br />
* [http://caiallen.tumblr.com/ '''Allen Cai''']:iGEM gave me hands-on experience on how it would be like to work in a real lab. I loved it and couldn't see myself doing anything else this summer than iGEM.<br />
* [http://cathyliu.tumblr.com/ '''Cathy Liu''']:This summer has been a lot of things. Demanding, stressful, tedious? Sure. But it has definitely been amazing, and a bunch of things I couldn’t express with words. I know we’re going to rock the Jamboree; it’s going to be epic. And I know I’ll only think of all the wonderful times when looking back at all this. There will always be a special place in my heart for UCSF. (P.S. “SUCCESS!”)<br />
* [http://miaomiao.tumblr.com/ '''Edna Miao''']: IGEM has forced me outside my comfort zone many times and consequently has taught me better public speaking and critical thinking skills, on top of new lab techniques of course.<br />
* [http://echan08.blogspot.com/ '''Ethan Chan''']: This experience has brought me closer to my peers and gave me critical lab experience that not many High School students get! <br />
* [http://ertwong.tumblr.com/ '''Eric Wong''']MADNESS? THIS IS RESEARCH!!! >:O well yeah i enjoyed my time at UCSF, I learned many new things such as lab techniques and chemotaxis as well as meet a bunch of people who i got along great with. CAZZO!!!<br />
* '''Hansi Liu'''<br />
* [http://tackiejam.tumblr.com/ '''Jackie Tam''']:Participating on UCSF’s iGEM team is unparalleled learning experience. This is my first formal lab experience, and I am incredibly thankful that it took place here at the Lim Lab at UCSF.<br />
* '''Katja Kolar'''<br />
* [http://ryanquan.tumblr.com/ '''Ryan Quan''']:This summer I learned many new techniques and how cells move, but the best thing was teaching other people techniques because it really makes you make sure you know your stuff before you open you explain something. <br />
* [http://ryeland.tumblr.com/ '''Ryan Liang''']:This summer has been engagingly educational and highly demanding as well as personally valuable in career-building.<br />
<br />
* [http://davidpincus.tumblr.com/ '''Buddy David Pincus's blog''']<br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}</div>EthanChanhttp://2009.igem.org/Cathy_LiuCathy Liu2009-10-20T16:29:23Z<p>EthanChan: </p>
<hr />
<div>{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
|}<br />
===The Human Organizer===<br />
<br />
My name is Cathy, and here's a nice chunky paragraph about me. I am currently a second-year student on the UCSF iGem team and will be a sophomore at the University of California, Los Angeles this Fall. Outside of the lab, you will find me with my closest friends. I am a huge movie junky and I spend a lot of time watching television now that the internet makes up for lack of cable. I like spending time with my boyfriend, baking for special occasions, and making birthday cards by hand. If you got an art project for me, I'll take it. I absolutely, positively love to eat. And I enjoy the company of the Food Network. I like funny people or just a good laugh. I like to shop. I really like the Harry Potter series. Last summer, I volunteered to present and the iGem jamboree and realized how much I like to deliver Powerpoint presentations. I like music. I like to play badminton. I like Fridays and Saturdays. I like the comfort of car rides. I like amusement parks and roller coasters. I like huge medical terms thrown around by doctors. I like to sleep. I like my cat. If I were not set on a profession in the scientific field, I would want to be a dancer. I like the night air in San Francisco. I love my home and the family that keeps it warm. Most of all, I like all the good stuff that has shaped my nineteen years so far. I’m excited for what this world has in store for me for the next however many years, and this Jamboree is just one of the gajillion things that I refer to. I now leave you with four epic letters: UCSF</div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T04:40:55Z<p>EthanChan: /* Introduction to the 2009 UCSF IGEM Team */</p>
<hr />
<div><br />
<br />
<br />
{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="1"|<br />
<br />
'''Advisors:'''<br />
*'''Wendell Lim'''<br />
*'''Orion Weiner''' <br />
*'''James Onuffer'''<br />
<br />
'''Educational Director'''<br />
*'''[[Raquel Gomes]]''' <br />
<br />
'''Scientific Directors'''<br />
*'''[[Ben Rhau]]''' <br />
*'''[[Oliver Hoeller]]''' <br />
<br />
'''Buddies:'''<br />
*'''[[Aynur Tasdemir]]''' <br />
*'''[[Jason Park]]''' <br />
*'''[[Andrew Houk]]''' <br />
*'''[[Delquin Gong]]''' <br />
*'''[[Arthur Millius]]''' <br />
*'''[[Bethany Simmons]]'''<br />
<br />
*'''[[Saber Khan]]''' <br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''High School Students:'''<br />
<br />
*'''[[Allen Cai]]''' <br />
*'''[[Alex Smith]]''' <br />
*'''[[Edna Miao]]''' <br />
*'''[[Ethan Chan]]''' <br />
*'''[[Eric Wong]]''' <br />
*'''[[Jackie Tam]]''' <br />
*'''[[Ryan Liang]]''' <br />
<br />
<br />
<br />
'''Undergrads:'''<br />
*'''[[Cathy Liu]]'''<br />
*'''[[Ryan Quan]]'''<br />
*'''[[Hansi Liu]]'''<br />
*'''[[Katja Kolar]]'''<br />
|<br />
<gallery><br />
Advisors<br />
Image:Wendell.JPG|Wendell Lim<br />
Image:Orion_weiner.gif|Orion Weiner<br />
Image:.|James Onuffer<br />
</gallery><br />
<br />
<gallery><br />
Educational Director<br />
Image:.|Raquel Gomes<br />
</gallery><br />
<br />
<gallery><br />
Scientific Directors<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
</gallery><br />
<br />
<gallery><br />
Buddies<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery><br />
<br />
<gallery><br />
High School Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
</gallery><br />
|}<br />
<br />
<gallery><br />
Under Grads<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery><br />
<br />
== '''What we did''' ==<br />
<br />
(Provide proper attribution for all work)<br />
<br />
<br />
== '''Where we're from''' ==<br />
<br />
Extra pages:<br />
[[subteam1]]</div>EthanChanhttp://2009.igem.org/File:Wendell.JPGFile:Wendell.JPG2009-10-20T04:39:07Z<p>EthanChan: </p>
<hr />
<div></div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T04:18:40Z<p>EthanChan: /* Introduction to the 2009 UCSF IGEM Team */</p>
<hr />
<div><br />
<br />
<br />
{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="1"|<br />
<br />
'''Advisors:'''<br />
*'''Wendell Lim'''<br />
*'''Orion Weiner''' <br />
*'''James Onuffer'''<br />
<br />
'''Educational Director'''<br />
*'''[[Raquel Gomes]]''' <br />
<br />
'''Scientific Directors'''<br />
*'''[[Ben Rhau]]''' <br />
*'''[[Oliver Hoeller]]''' <br />
<br />
'''Buddies:'''<br />
*'''[[Aynur Tasdemir]]''' <br />
*'''[[Jason Park]]''' <br />
*'''[[Andrew Houk]]''' <br />
*'''[[Delquin Gong]]''' <br />
*'''[[Arthur Millius]]''' <br />
*'''[[Bethany Simmons]]'''<br />
<br />
*'''[[Saber Khan]]''' <br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''High School Students:'''<br />
<br />
*'''[[Allen Cai]]''' <br />
*'''[[Alex Smith]]''' <br />
*'''[[Edna Miao]]''' <br />
*'''[[Ethan Chan]]''' <br />
*'''[[Eric Wong]]''' <br />
*'''[[Jackie Tam]]''' <br />
*'''[[Ryan Liang]]''' <br />
<br />
<br />
<br />
'''Undergrads:'''<br />
*'''[[Cathy Liu]]'''<br />
*'''[[Ryan Quan]]'''<br />
*'''[[Hansi Liu]]'''<br />
*'''[[Katja Kolar]]'''<br />
|<br />
<gallery><br />
Advisors<br />
Image:.|Wendell Lim<br />
Image:Orion_weiner.gif|Orion Weiner<br />
Image:.|James Onuffer<br />
</gallery><br />
<br />
<gallery><br />
Educational Director<br />
Image:.|Raquel Gomes<br />
</gallery><br />
<br />
<gallery><br />
Scientific Directors<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
</gallery><br />
<br />
<gallery><br />
Buddies<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery><br />
<br />
<gallery><br />
High School Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
</gallery><br />
|}<br />
<br />
<gallery><br />
Under Grads<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery><br />
<br />
== '''What we did''' ==<br />
<br />
(Provide proper attribution for all work)<br />
<br />
<br />
== '''Where we're from''' ==<br />
<br />
Extra pages:<br />
[[subteam1]]</div>EthanChanhttp://2009.igem.org/File:Orion_weiner.gifFile:Orion weiner.gif2009-10-20T04:17:35Z<p>EthanChan: </p>
<hr />
<div></div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T04:16:12Z<p>EthanChan: /* Introduction to the 2009 UCSF IGEM Team */</p>
<hr />
<div><br />
<br />
<br />
{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="1"|<br />
<br />
'''Advisors:'''<br />
*'''Wendell Lim'''<br />
*'''Orion Weiner''' <br />
*'''James Onuffer'''<br />
<br />
'''Educational Director'''<br />
*'''[[Raquel Gomes]]''' <br />
<br />
'''Scientific Directors'''<br />
*'''[[Ben Rhau]]''' <br />
*'''[[Oliver Hoeller]]''' <br />
<br />
'''Buddies:'''<br />
*'''[[Aynur Tasdemir]]''' <br />
*'''[[Jason Park]]''' <br />
*'''[[Andrew Houk]]''' <br />
*'''[[Delquin Gong]]''' <br />
*'''[[Arthur Millius]]''' <br />
*'''[[Bethany Simmons]]'''<br />
<br />
*'''[[Saber Khan]]''' <br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''High School Students:'''<br />
<br />
*'''[[Allen Cai]]''' <br />
*'''[[Alex Smith]]''' <br />
*'''[[Edna Miao]]''' <br />
*'''[[Ethan Chan]]''' <br />
*'''[[Eric Wong]]''' <br />
*'''[[Jackie Tam]]''' <br />
*'''[[Ryan Liang]]''' <br />
<br />
<br />
<br />
'''Undergrads:'''<br />
*'''[[Cathy Liu]]'''<br />
*'''[[Ryan Quan]]'''<br />
*'''[[Hansi Liu]]'''<br />
*'''[[Katja Kolar]]'''<br />
|<br />
<gallery><br />
Advisors<br />
Image:.|Wendell Lim<br />
Image:.|James Onuffer<br />
Image:.|Orion Weiner<br />
</gallery><br />
<br />
<gallery><br />
Educational Director<br />
Image:.|Raquel Gomes<br />
</gallery><br />
<br />
<gallery><br />
Scientific Directors<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
</gallery><br />
<br />
<gallery><br />
Buddies<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery><br />
<br />
<gallery><br />
High School Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
</gallery><br />
|}<br />
<br />
<gallery><br />
Under Grads<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery><br />
<br />
== '''What we did''' ==<br />
<br />
(Provide proper attribution for all work)<br />
<br />
<br />
== '''Where we're from''' ==<br />
<br />
Extra pages:<br />
[[subteam1]]</div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T04:06:58Z<p>EthanChan: /* Introduction to the 2009 UCSF IGEM Team */</p>
<hr />
<div><br />
<br />
<br />
{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="1"|<br />
<br />
'''Advisors:'''<br />
*'''Wendell Lim'''<br />
*'''Orion Weiner''' <br />
*'''James Onuffer'''<br />
<br />
'''Educational Director'''<br />
*'''[[Raquel Gomes]]''' <br />
<br />
'''Scientific Directors'''<br />
*'''[[Ben Rhau]]''' <br />
*'''[[Oliver Hoeller]]''' <br />
<br />
'''Buddies:'''<br />
*'''[[Aynur Tasdemir]]''' <br />
*'''[[Jason Park]]''' <br />
*'''[[Andrew Houk]]''' <br />
*'''[[Delquin Gong]]''' <br />
*'''[[Arthur Millius]]''' <br />
*'''[[Bethany Simmons]]'''<br />
<br />
*'''[[Saber Khan]]''' <br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''High School Students:'''<br />
<br />
*'''[[Allen Cai]]''' <br />
*'''[[Alex Smith]]''' <br />
*'''[[Edna Miao]]''' <br />
*'''[[Ethan Chan]]''' <br />
*'''[[Eric Wong]]''' <br />
*'''[[Jackie Tam]]''' <br />
*'''[[Ryan Liang]]''' <br />
<br />
<br />
<br />
'''Undergrads:'''<br />
*'''[[Cathy Liu]]'''<br />
*'''[[Ryan Quan]]'''<br />
*'''[[Hansi Liu]]'''<br />
*'''[[Katja Kolar]]'''<br />
|<br />
<gallery><br />
Advisors<br />
Image:.|Wendell Lim<br />
Image:.|James Onuffer<br />
Image:.|Orion Weiner<br />
</gallery><br />
<br />
<gallery><br />
Education Director<br />
Image:.|Raquel Gomes<br />
</gallery><br />
<br />
<gallery><br />
Scientific Directors<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
</gallery><br />
<br />
<gallery><br />
Buddies<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery><br />
<br />
<gallery><br />
High School Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
</gallery><br />
|}<br />
<br />
<gallery><br />
Under Grads<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery><br />
<br />
== '''What we did''' ==<br />
<br />
(Provide proper attribution for all work)<br />
<br />
<br />
== '''Where we're from''' ==<br />
<br />
Extra pages:<br />
[[subteam1]]</div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T03:31:22Z<p>EthanChan: /* Introduction to the 2009 UCSF IGEM Team */</p>
<hr />
<div><br />
<br />
<br />
{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="1"|<br />
<br />
'''Advisors:'''<br />
*'''Wendell Lim'''<br />
*'''Orion Weiner''' <br />
*'''James Onuffer'''<br />
<br />
'''Educational Director'''<br />
*'''[[Raquel Gomes]]''' <br />
<br />
'''Scientific Directors'''<br />
*'''[[Ben Rhau]]''' <br />
*'''[[Oliver Hoeller]]''' <br />
<br />
'''Buddies:'''<br />
*'''[[Aynur Tasdemir]]''' <br />
*'''[[Jason Park]]''' <br />
*'''[[Andrew Houk]]''' <br />
*'''[[Delquin Gong]]''' <br />
*'''[[Arthur Millius]]''' <br />
*'''[[Bethany Simmons]]'''<br />
<br />
*'''[[Saber Khan]]''' <br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''High School Students:'''<br />
<br />
*'''[[Allen Cai]]''' <br />
*'''[[Alex Smith]]''' <br />
*'''[[Edna Miao]]''' <br />
*'''[[Ethan Chan]]''' <br />
*'''[[Eric Wong]]''' <br />
*'''[[Jackie Tam]]''' <br />
*'''[[Ryan Liang]]''' <br />
<br />
<br />
<br />
'''Undergrads:'''<br />
*'''[[Cathy Liu]]'''<br />
*'''[[Ryan Quan]]'''<br />
*'''[[Hansi Liu]]'''<br />
*'''[[Katja Kolar]]'''<br />
|<br />
<gallery><br />
Buddies<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery><br />
<br />
<gallery><br />
High School Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
</gallery><br />
|}<br />
<br />
<gallery><br />
Under Grads<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery><br />
<br />
== '''What we did''' ==<br />
<br />
(Provide proper attribution for all work)<br />
<br />
<br />
== '''Where we're from''' ==<br />
<br />
Extra pages:<br />
[[subteam1]]</div>EthanChanhttp://2009.igem.org/Team:UCSF/ProjectTeam:UCSF/Project2009-10-20T00:20:17Z<p>EthanChan: /* Methods */</p>
<hr />
<div>{|align="justify"<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
<br />
== '''Overall project''' ==<br />
<br />
Your abstract<br />
<br />
== '''Part 1: Navigation: rewiring the cell to move toward new chemical signals'''==<br />
<br />
=== Motivation: ''why is this useful?'' ===<br />
We envision a cellular robot that could travel to practically any site in the human body. This would provide a flexible platform that could be used for a variety of therapeutic tasks. The first step toward achieving this goal is to broaden the range of possible chemotactic targets for our cells. Ideally, we could connect virtually any input to chemotaxis in a generalized way.<br />
<br />
=== Approach: ===<br />
Neutrophils sense most of their chemotactic signals through [http://en.wikipedia.org/wiki/G_protein_coupled_receptors/ G protein-coupled receptors] (GPCRs). The spectrum of chemical signals to which these cells respond is therefore determined, at least in part, by the set of GPCRs they express. Can this spectrum be broadened arbitrarily by the introduction of new GPCRs? We tested this idea by expressing 23 exogenous GPCRs in HL-60 (neutrophil-like) cells.<br />
<br />
== '''Part 2: Speed: engineering accelerators and brakes: A cellular cruise control by modulating cell polarity with feedback loops''' ==<br />
<br />
=== Motivation: ''why is this useful?'' ===<br />
Just like we have control over speed in a car – we can brake or accelerate – it would be useful to engineer such behavior into our cellular nanorobots.<br />
Just think about it: We could '''speed cells up''' so that they reach their targets faster and '''stop them''' once they have arrived or do not behave properly.<br />
<br />
=== Background: ===<br />
For these experiments we chose ''Dictyostelium discoideum'' cells to test our prototypical brakes and accelerators quickly. We expect that our brakes and accelerators can be used in a plug and play fashion because Dicty’s way of movement is very similar to a neutrophil’s:<br />
<br />
When a receptor binds chemoattractant, it induces the conversion of PIP2 to PIP3 (two signaling lipids in the plasma membrane) at the front of our cells. In a '''positive feedback loop''' PIP3 triggers the formation of more PIP3 at the front while similarly PIP2 leads to more PIP2 production at the sides and rear of the cell. This system sets the axis of polarity of the cell. The PIP3 patch at the front functions as a ‘turbo boost’ pushing the cell forward.<br />
<br />
<br />
[[Image:BACKGROUND1.jpg|500px|thumb|center|'''Polarized distribution of PIP3 and PIP2:''' A patch of PIP3 is localized at the front of a cell while PIP2 is at the back. Feedback loops are used to establish and maintain this polarized distribution. ]]<br />
<br />
=== Approach: ===<br />
Inspired by nature we tried to build accelerators and brakes by introducing our own synthetic protein based feedback loops. We designed feedback elements by fusing localization and catalytic domains involved in PIP3 production and degradation to modulate localization and level of PIP3 and PIP2 in the cell. <br />
<br />
Here is an example of a positive feedback loop: a PIP3 binding localization domain fused to a PIP3 producing catalytic domain could produce more PIP3 where there is already PIP3- at the front. This might strengthen polarity and accelerate a cell.<br />
<br />
[[Image:positive_feedback_loop2.jpg|500px|thumb|center|'''Synthetic protein based feedback loops:''' One example: a positive feedback loop comprised of a PIP3 binding domain fused to a PIP3 generating enzyme.]]<br />
<br />
=== Results: ===<br />
Over the summer we assembled more than 100 fusions of localization and catalytic domains and screened whether they work. How? We measured the effect our constructs have on motility of ''Dictyostelium'' cells: stronger polarity should make cells faster while weaker polarity ought to slow them down!<br />
<br />
[[Here]] is an overview of all feedback loops we screened and the effect they had on the speed of cells. If you are interested what all the elements are check out our biobricks.<br />
We used automated cell tracking on more than 196 hours worth of movies (note: one movie is 10 minutes!) and identified strains that moved faster or slower at a very stringent statistical cutoff (p<0.0001). <br />
<br />
<br />
This way we were able to identify 7 brakes and 1 accelerator!<br />
<br />
Check out the movie of '''one of our strong brakes (PTEN-RasC const. active) compared to wildtype'''. We hypothesize that this construct acts as a negative feedback loop on PIP2- (generating PIP3 where PIP2 should be) thereby confusing the cell with multiple fronts:<br />
<br />
[[Image:HO230.jpg|450px|thumb|right|PTEN (PIP2 binding) fused to RasC da (PIP3 generating)]] [[Image:wt.jpg|450px|thumb|left|wildtype]]<br />
<br />
This is indeed an effect of fusing the particular localization to the catalytic domain as neither of them alone has such a strong effect ([[see details]]).<br />
<br />
=== Summary and outlook: ===<br />
We have screened more than 100 synthetic feedback elements for their ability to accelerate or slow down speed of cell motility. We have isolated a hand full of functional elements. Now we need to confirm the mechanism of action of these elements. In the future we would like to make them inducible by a signal from outside – like a stoplight!<br />
<br />
===Methods===<br />
*[[Media:Aar1_shuffle.pdf|Cloning: Aar1 Shuffle]] - Connecting all our fragments together.<br />
*[[Media:DICTY TRANSFORMATION.pdf|Dicty: transformation protocol]] - Used to transform into dicty<br />
*[[Media:DICTY-analyzing_motility.pdf|Dicty: Analyzing Motility protocol]] - Used to analyze the basic moitily of all cells</div>EthanChanhttp://2009.igem.org/Team:UCSF/TeamTeam:UCSF/Team2009-10-20T00:10:40Z<p>EthanChan: /* Introduction to the 2009 UCSF IGEM Team */</p>
<hr />
<div><br />
<br />
<br />
{|align="justify"<br />
|[[Image:Igemteamucsf.jpg|800px|thumb|right|Team UCSF]] <br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Introduction to the 2009 UCSF IGEM Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="3"|<br />
<br />
<br />
'''Advisors:'''<br />
*'''Wendell Lim'''<br />
*'''Orion Weiner''' <br />
*'''James Onuffer'''<br />
<br />
'''Educational Director'''<br />
*'''[[Raquel Gomes]]''' <br />
<br />
'''Scientific Directors'''<br />
*'''[[Ben Rhau]]''' <br />
*'''[[Oliver Hoeller]]''' <br />
<br />
'''Buddies:'''<br />
*'''[[Aynur Tasdemir]]''' <br />
*'''[[Jason Park]]''' <br />
*'''[[Andrew Houk]]''' <br />
*'''[[Delquin Gong]]''' <br />
*'''[[Arthur Millius]]''' <br />
*'''[[Bethany Simmons]]'''<br />
<br />
*'''[[Saber Khan]]''' <br />
<br />
<br />
'''High School Students:'''<br />
<br />
*'''[[Allen Cai]]''' <br />
*'''[[Alex Smith]]''' <br />
*'''[[Edna Miao]]''' <br />
*'''[[Ethan Chan]]''' <br />
*'''[[Eric Wong]]''' <br />
*'''[[Jackie Tam]]''' <br />
*'''[[Ryan Liang]]''' <br />
<br />
<br />
<br />
'''Undergrads:'''<br />
*'''[[Cathy Liu]]'''<br />
*'''[[Ryan Quan]]'''<br />
*'''[[Hansi Liu]]'''<br />
*'''[[Katja Kolar]]'''<br />
|<br />
<gallery><br />
Buddies<br />
Image:AYNUR.JPG|Aynur Tasdemir<br />
Image:Ben_R.gif|Ben Rhau<br />
Image:Oliver_H.gif|Oliver Hoeller<br />
Image:Jason_P.jpg|Jason Park<br />
Image:Andrew_H.gif|Andrew Houk<br />
Image:Delquin_G.gif|Delquin Gong<br />
Image:Arthur_M.gif|Arthur Millius<br />
Image:Bethany_s.png|Bethany Simmons<br />
Image:Saber_Khan.jpg|Saber Khan<br />
</gallery><br />
<br />
<gallery><br />
High School Students<br />
Image:Allen_C.jpg|Allen Cai<br />
Image:Alex_Smith.jpg|Alex Smith<br />
Image:Edna_Miao.jpg|Edna Miao<br />
Image:Ethan_C.jpg|Ethan Chan<br />
Image:Eric_W.jpg|Eric Wong<br />
Image:Jackie_T.jpg|Jackie Tam<br />
Image:Ryan_L.jpg|Ryan Liang<br />
</gallery><br />
|}<br />
<br />
<gallery><br />
Under Grads<br />
Image:Cathy_Liu.jpg|Cathy Liu<br />
Image:Ryan_Q.jpg|Ryan Quan<br />
Image:Hansi_L.jpg|Hansi Liu<br />
Image:Katja_K.jpg|Katja Kolar<br />
</gallery><br />
<br />
== '''What we did''' ==<br />
<br />
(Provide proper attribution for all work)<br />
<br />
<br />
== '''Where we're from''' ==<br />
<br />
Extra pages:<br />
[[subteam1]]</div>EthanChanhttp://2009.igem.org/Saber_KhanSaber Khan2009-10-20T00:10:08Z<p>EthanChan: </p>
<hr />
<div>{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
|}<br />
===The Teacher===</div>EthanChanhttp://2009.igem.org/Bethany_SimmonsBethany Simmons2009-10-20T00:09:54Z<p>EthanChan: </p>
<hr />
<div>{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
|}<br />
===The Athlete===</div>EthanChan