http://2009.igem.org/wiki/index.php?title=Special:Contributions/Lmccomm2&feed=atom&limit=50&target=Lmccomm2&year=&month=2009.igem.org - User contributions [en]2024-03-28T20:51:06ZFrom 2009.igem.orgMediaWiki 1.16.5http://2009.igem.org/Team:Illinois/3%27-Acting_sRNAsTeam:Illinois/3'-Acting sRNAs2009-07-10T15:31:45Z<p>Lmccomm2: /* mRNA Degradation Team */</p>
<hr />
<div>{{UIUC09Temp}}<br />
{{Illinois}}<br />
{{IllNoteNav}}<br />
<br />
<br />
== '''mRNA Degradation Team''' ==<br />
<br />
'''Goals:'''<br />
<br />
Our goal is to work with sRNAs that bind to the 3' UTR. We will study this regulation system using the Vogel protocol. We want to have two sRNAs controlling one gene, which could be accomplished through using a combination of 5' and 3' acting sRNAs.<br />
<br />
'''Background:'''<br />
<br />
Colony Boil-Streak plate E. Coli K12 on LB agar, incubate overnight at 37C. Pick healthy colony with sterile glass pasteur pipette and innoculate 100 uL dH20. Boil for 10 min, centrifugate for 5 min at 14K rpm. Extract 1 uL of supernatant as template DNA for PCR.<br />
<br />
'''GadY Gene Sequence:''' (K-12 substr. MG1655: 3662867-3663047)<br />
<br />
ACTGAGAGCA CAAAGTTTCC CGTGCCAACA GGGAGTGTTA TAACGGTTTA TTAGTCTGGA GACGGCAGAC TATCCTCTTC CCGGTCCCCT ATGCCGGGTT TTTTTTATGT CTGAGTAAAA CTCTATAATC TTATTCCTTC<br />
<br />
'''GadX 5' UTR Sequence:''' (K-12 substr. MG1655: 3662641-3662717)<br />
<br />
AGTTTTACTC AGACATAAAA AAAACCCGGC ATAGGGGACC GGGAAGAGGA TAGTCTGCCG TCTCCAGACT AATAAA <br />
<br />
'''GadX 5' UTR Sequence (2):''' (K-12 substr. MG1655: 3662641-3663008)<br />
<br />
AGTTTTACTC AGACATAAAA AAAACCCGGC ATAGGGGACC GGGAAGAGGA TAGTCTGCCG TCTCCAGACT AATAAACCGT TATAACACTC CCTGTTGGCA CGGGAAACTT TGTGCTCTCA GTAAGTTAAA TATAACTTTT ACTGGAAATA AGATCAGCCA TTTTTTTATA AACATAAGCT ATACGCTGTG CGAAAATATA TTCGTGCTGC ATTTACTTAT TATCAATTAA CTGTTATGCA AAACTACTTT GTGGATAAAT TTTGGTCCTA CCAAATCTGG CAGTTTTTGC GCTAAGAAAC AGTCTGGCAT CATTTCATTA GTATACTGAA ATTGAAATAA TCGCAGTATG AAATATAAGG GATAATC<br />
<br />
'''Primers Used:'''<br />
<br />
*Forward/sense gadY (sRNA gene) primer: ACTGAGAGCACAAAGTTTCC<br />
*Reverse/antisense gadY (sRNA gene) primer: (XbaI)-GTTTTTTCTAGAGAAGGAATAAGATTATAGAG<br />
*Forward/sense gadX (sRNA target sequence) primer: (SwaI)-GTTTTTATTTAAATAGTTTTACTCAGACATAAAAAAAACC<br />
*Reverse/antisense gadX (sRNA target sequence) primer: (XbaI)-GTTTTTTCTAGATTTATTAGTCTGGAGACGGCAG<br />
*Reverse/antisense gadX (sRNA target sequence) primer 2: (XbaI)-GTTTTTTCTAGAGATTATCCCTTATATTTCATAC<br />
<br />
'''PCR Products:'''<br />
<br />
GadY GENE (XbaI restriction site)-152bp <br />
<br />
GadX 3' UTR (SwaI, XbaI restriction sites)-102bp, 393bp :<br />
<br />
<br />
'''July 9:'''<br />
We cloned the GadY gene as well as the 3' UTR of gadX. We used two different primers for gadX because we weren't sure which design would work the best. Primer 2 goes all the way to the start codon of gadW. <br />
<br />
We did this with a successful PCR.<br />
<br />
[[Image:IllinoisiGEM_gadY_gel1.jpg]]<br />
<br />
<br />
We then purified the PCR product that was not used in the gel. We also prepared an overnight of dh5ap. transformed with pXG-1.<br />
<br />
'''July 10:'''<br />
<br />
Today we performed a midiprep to extract the plasmid DNA from dH5ap.</div>Lmccomm2http://2009.igem.org/Team:Illinois/3%27-Acting_sRNAsTeam:Illinois/3'-Acting sRNAs2009-07-10T15:30:52Z<p>Lmccomm2: /* mRNA Degradation Team */</p>
<hr />
<div>{{UIUC09Temp}}<br />
{{Illinois}}<br />
{{IllNoteNav}}<br />
<br />
<br />
== '''mRNA Degradation Team''' ==<br />
<br />
'''Goals:'''<br />
<br />
Our goal is to work with sRNAs that bind to the 3' UTR. We will study this regulation system using the Vogel protocol. We want to have two sRNAs controlling one gene, which could be accomplished through using a combination of 5' and 3' acting sRNAs.<br />
<br />
'''Background:'''<br />
<br />
Colony Boil-Streak plate E. Coli K12 on LB agar, incubate overnight at 37C. Pick healthy colony with sterile glass pasteur pipette and innoculate 100 uL dH20. Boil for 10 min, centrifugate for 5 min at 14K rpm. Extract 1 uL of supernatant as template DNA for PCR.<br />
<br />
'''GadY Gene Sequence:''' (K-12 substr. MG1655: 3662867-3663047)<br />
<br />
ACTGAGAGCA CAAAGTTTCC CGTGCCAACA GGGAGTGTTA TAACGGTTTA TTAGTCTGGA GACGGCAGAC TATCCTCTTC CCGGTCCCCT ATGCCGGGTT TTTTTTATGT CTGAGTAAAA CTCTATAATC TTATTCCTTC<br />
<br />
'''GadX 5' UTR Sequence:''' (K-12 substr. MG1655: 3662641-3662717)<br />
<br />
AGTTTTACTC AGACATAAAA AAAACCCGGC ATAGGGGACC GGGAAGAGGA TAGTCTGCCG TCTCCAGACT AATAAA <br />
<br />
'''GadX 5' UTR Sequence (2):''' (K-12 substr. MG1655: 3662641-3663008)<br />
<br />
AGTTTTACTC AGACATAAAA AAAACCCGGC ATAGGGGACC GGGAAGAGGA TAGTCTGCCG TCTCCAGACT AATAAACCGT TATAACACTC CCTGTTGGCA CGGGAAACTT TGTGCTCTCA GTAAGTTAAA TATAACTTTT ACTGGAAATA AGATCAGCCA TTTTTTTATA AACATAAGCT ATACGCTGTG CGAAAATATA TTCGTGCTGC ATTTACTTAT TATCAATTAA CTGTTATGCA AAACTACTTT GTGGATAAAT TTTGGTCCTA CCAAATCTGG CAGTTTTTGC GCTAAGAAAC AGTCTGGCAT CATTTCATTA GTATACTGAA ATTGAAATAA TCGCAGTATG AAATATAAGG GATAATC<br />
<br />
'''Primers Used:'''<br />
<br />
*Forward/sense gadY (sRNA gene) primer: ACTGAGAGCACAAAGTTTCC<br />
*Reverse/antisense gadY (sRNA gene) primer: (XbaI)-GTTTTTTCTAGAGAAGGAATAAGATTATAGAG<br />
*Forward/sense gadX (sRNA target sequence) primer: (SwaI)-GTTTTTATTTAAATAGTTTTACTCAGACATAAAAAAAACC<br />
*Reverse/antisense gadX (sRNA target sequence) primer: (XbaI)-GTTTTTTCTAGATTTATTAGTCTGGAGACGGCAG<br />
*Reverse/antisense gadX (sRNA target sequence) primer 2: (XbaI)-GTTTTTTCTAGAGATTATCCCTTATATTTCATAC<br />
<br />
'''PCR Products:'''<br />
<br />
GadY GENE (XbaI restriction site)-152bp <br />
<br />
GadX 3' UTR (SwaI, XbaI restriction sites)-102bp, 393bp :<br />
<br />
<br />
'''July 9:'''<br />
We cloned the GadY gene as well as the 3' UTR of gadX. We used two different primers for gadX because we weren't sure which design would work the best. Primer 2 goes all the way to the start codon of gadW. <br />
<br />
We did this with a successful PCR.<br />
<br />
[[Image:IllinoisIGEM_gadY_gel1.jpg]<br />
<br />
We then purified the PCR product that was not used in the gel. We also prepared an overnight of dh5ap. transformed with pXG-1.<br />
<br />
'''July 10:'''<br />
<br />
Today we performed a midiprep to extract the plasmid DNA from dH5ap.</div>Lmccomm2http://2009.igem.org/Team:Illinois/3%27-Acting_sRNAsTeam:Illinois/3'-Acting sRNAs2009-07-10T15:16:37Z<p>Lmccomm2: /* mRNA Degradation Team */</p>
<hr />
<div>{{UIUC09Temp}}<br />
{{Illinois}}<br />
{{IllNoteNav}}<br />
<br />
<br />
== '''mRNA Degradation Team''' ==<br />
<br />
'''Goals:'''<br />
<br />
Our goal is to work with sRNAs that bind to the 3' UTR. We will study this regulation system using the Vogel protocol. We want to have two sRNAs controlling one gene, which could be accomplished through using a combination of 5' and 3' acting sRNAs.<br />
<br />
'''Background:'''<br />
<br />
Colony Boil-Streak plate E. Coli K12 on LB agar, incubate overnight at 37C. Pick healthy colony with sterile glass pasteur pipette and innoculate 100 uL dH20. Boil for 10 min, centrifugate for 5 min at 14K rpm. Extract 1 uL of supernatant as template DNA for PCR.<br />
<br />
'''GadY Gene Sequence:''' (K-12 substr. MG1655: 3662867-3663047)<br />
<br />
ACTGAGAGCA CAAAGTTTCC CGTGCCAACA GGGAGTGTTA TAACGGTTTA TTAGTCTGGA GACGGCAGAC TATCCTCTTC CCGGTCCCCT ATGCCGGGTT TTTTTTATGT CTGAGTAAAA CTCTATAATC TTATTCCTTC<br />
<br />
'''GadX 5' UTR Sequence:''' (K-12 substr. MG1655: 3662641-3662717)<br />
<br />
AGTTTTACTC AGACATAAAA AAAACCCGGC ATAGGGGACC GGGAAGAGGA TAGTCTGCCG TCTCCAGACT AATAAA <br />
<br />
'''GadX 5' UTR Sequence (2):''' (K-12 substr. MG1655: 3662641-3663008)<br />
<br />
AGTTTTACTC AGACATAAAA AAAACCCGGC ATAGGGGACC GGGAAGAGGA TAGTCTGCCG TCTCCAGACT AATAAACCGT TATAACACTC CCTGTTGGCA CGGGAAACTT TGTGCTCTCA GTAAGTTAAA TATAACTTTT ACTGGAAATA AGATCAGCCA TTTTTTTATA AACATAAGCT ATACGCTGTG CGAAAATATA TTCGTGCTGC ATTTACTTAT TATCAATTAA CTGTTATGCA AAACTACTTT GTGGATAAAT TTTGGTCCTA CCAAATCTGG CAGTTTTTGC GCTAAGAAAC AGTCTGGCAT CATTTCATTA GTATACTGAA ATTGAAATAA TCGCAGTATG AAATATAAGG GATAATC<br />
<br />
'''Primers Used:'''<br />
<br />
*Forward/sense gadY (sRNA gene) primer: ACTGAGAGCACAAAGTTTCC<br />
*Reverse/antisense gadY (sRNA gene) primer: (XbaI)-GTTTTTTCTAGAGAAGGAATAAGATTATAGAG<br />
*Forward/sense gadX (sRNA target sequence) primer: (SwaI)-GTTTTTATTTAAATAGTTTTACTCAGACATAAAAAAAACC<br />
*Reverse/antisense gadX (sRNA target sequence) primer: (XbaI)-GTTTTTTCTAGATTTATTAGTCTGGAGACGGCAG<br />
*Reverse/antisense gadX (sRNA target sequence) primer 2: (XbaI)-GTTTTTTCTAGAGATTATCCCTTATATTTCATAC<br />
<br />
'''PCR Products:'''<br />
<br />
GadY GENE (XbaI restriction site)-152bp <br />
<br />
GadX 3' UTR (SwaI, XbaI restriction sites)-102bp, 393bp :<br />
<br />
<br />
'''July 9:'''<br />
We cloned the GadY gene as well as the 3' UTR of gadX. We used two different primers for gadX because we weren't sure which design would work the best. Primer 2 goes all the way to the start codon of gadW. <br />
<br />
We did this with a successful PCR.<br />
<br />
pic.<br />
<br />
We then purified the PCR product that was not used in the gel. We also prepared an overnight of dh5ap. transformed with pXG-1.<br />
<br />
'''July 10:'''<br />
<br />
Today we performed a midiprep to extract the plasmid DNA from dH5ap.</div>Lmccomm2http://2009.igem.org/Team:Illinois/3%27-Acting_sRNAsTeam:Illinois/3'-Acting sRNAs2009-07-10T14:44:23Z<p>Lmccomm2: /* mRNA Degradation Team */</p>
<hr />
<div>{{UIUC09Temp}}<br />
{{Illinois}}<br />
{{IllNoteNav}}<br />
<br />
<br />
== '''mRNA Degradation Team''' ==<br />
<br />
'''Goals:'''<br />
<br />
Our goal is to work with sRNAs that bind to the 3' UTR. We will study this regulation system using the Vogel protocol. We want to have two sRNAs controlling one gene, which could be accomplished through using a combination of 5' and 3' acting sRNAs.</div>Lmccomm2http://2009.igem.org/Team:Illinois/MicATeam:Illinois/MicA2009-06-17T16:25:41Z<p>Lmccomm2: /* MicA Target-GFP Fusion */</p>
<hr />
<div>{{UIUC09Temp}}<br />
{{Illinois}}<br />
<br />
== '''MicA Target-GFP Fusion''' ==<br />
<br />
'''Purpose:''' to test the efficiency of sRNA repression. This will be accomplished by forming two plasmids by ligating the target sequence of the sRNA onto the pXG-10 plasmid behind the reporter gene GFP and ligating the sRNA gene into the pJU-334 DNA strand. These plasmids will then be transformed into E. coli and the fluorescence measured with a plate reader.<br />
<br />
'''Protocol(s) Used:''' The protocol we used is that taken from Urban and Vogel, "A Green Fluorescent Protein (GFP)-Based Plasmid System to Study Post-Transcriptional Control of Gene Expression In Vivo"<br />
<br />
'''Recipe(s) Used:''' <br />
<br />
'''Primers Used:'''<br />
<br />
== '''June 12''' ==<br />
<br />
Journal entry text goes here.<br />
<br />
== '''June 16'''==<br />
<br />
We used PCR to extract the sRNA gene: MicA and target sequence : ompA. from the e.coli chromosome. We then ran a gel to make sure that we had the right DNA fragments. Our results corresponded to our predictions. <br />
<br />
[[Image:illinoisgelofmicA/OmpA.jpg]]<br />
<br />
=='''June 17'''==<br />
<br />
We are completing a digestion of MicA, OmpA, and OmpF. Following the digestion we will incubate with SAP and then run a gel to verify the digestion. We will then extract the DNA for the sRNA target sequence and sRNA gene.</div>Lmccomm2http://2009.igem.org/Team:Illinois/MicATeam:Illinois/MicA2009-06-17T16:17:01Z<p>Lmccomm2: /* June 16 */</p>
<hr />
<div>{{UIUC09Temp}}<br />
{{Illinois}}<br />
<br />
== '''MicA Target-GFP Fusion''' ==<br />
<br />
'''Purpose:''' <br />
<br />
'''Protocol(s) Used:''' (links to protocols page)<br />
<br />
'''Recipe(s) Used:''' <br />
<br />
'''Primers Used:'''<br />
<br />
== '''June 12''' ==<br />
<br />
Journal entry text goes here.<br />
<br />
== '''June 16'''==<br />
<br />
We used PCR to extract the sRNA gene: MicA and target sequence : ompA. from the e.coli chromosome. We then ran a gel to make sure that we had the right DNA fragments. Our results corresponded to our predictions. <br />
<br />
[[Image:illinoisgelofmicA/OmpA.jpg]]<br />
<br />
=='''June 17'''==<br />
<br />
We are completing a digestion of MicA, OmpA, and OmpF. Following the digestion we will incubate with SAP and then run a gel to verify the digestion. We will then extract the DNA for the sRNA target sequence and sRNA gene.</div>Lmccomm2http://2009.igem.org/Team:Illinois/MicATeam:Illinois/MicA2009-06-17T16:16:10Z<p>Lmccomm2: /* June 12 */</p>
<hr />
<div>{{UIUC09Temp}}<br />
{{Illinois}}<br />
<br />
== '''MicA Target-GFP Fusion''' ==<br />
<br />
'''Purpose:''' <br />
<br />
'''Protocol(s) Used:''' (links to protocols page)<br />
<br />
'''Recipe(s) Used:''' <br />
<br />
'''Primers Used:'''<br />
<br />
== '''June 12''' ==<br />
<br />
Journal entry text goes here.<br />
<br />
== '''June 16'''==<br />
<br />
We used PCR to extract the sRNA gene: MicA and target sequence : ompA. from the e.coli chromosome. We then ran a gel to make sure that we had the right DNA fragments. Our results corresponded to our predictions. <br />
<br />
[[Image:Example.jpg]]<br />
<br />
=='''June 17'''==<br />
<br />
We are completing a digestion of MicA, OmpA, and OmpF. Following the digestion we will incubate with SAP and then run a gel to verify the digestion. We will then extract the DNA for the sRNA target sequence and sRNA gene.</div>Lmccomm2http://2009.igem.org/User:Lmccomm2User:Lmccomm22009-06-10T15:47:29Z<p>Lmccomm2: /* Lucy McCommas */</p>
<hr />
<div>{{UIUC09Temp}}<br />
{{Illinois}}<br />
<br />
== Lucy McCommas ==<br />
<br />
{|class="wikitable" border="0" width="800px" cellpadding="1" cellspacing="1" align="center"<br />
|- <br />
|width="400px"|'''Name:''' Lucy McCommas<br />
|width="400px" rowspan="7" valign="top"|[[Image:illinois lucy pic.jpg]]<br />
|-<br />
|'''Email:''' [mailto:lmccomm2@uiuc.edu lmccomm2@uiuc.edu ]<br />
|- <br />
|'''Home Town:''' Edwardsville, IL<br />
|-<br />
|'''Major:''' Molecular and Cellular Biology<br />
|-<br />
|'''Year:''' Senior<br />
|-<br />
|'''Research/Academic Interests:''' Synthetic Biology, dentistry<br />
|-<br />
|'''About Myself:''' I am very excited to be a part of the Illinois iGEM team this year! As a hobby I enjoy playing tennis.<br />
|}</div>Lmccomm2http://2009.igem.org/User:Lmccomm2User:Lmccomm22009-06-10T15:46:11Z<p>Lmccomm2: /* Lucy McCommas */</p>
<hr />
<div>{{UIUC09Temp}}<br />
{{Illinois}}<br />
<br />
== Lucy McCommas ==<br />
<br />
{|class="wikitable" border="0" width="800px" cellpadding="1" cellspacing="1" align="center"<br />
|- <br />
|width="400px"|'''Name:''' Lucy McCommas<br />
|width="400px" rowspan="7" valign="top"|[[Image:illinois lucy pic.jpg]]<br />
|-<br />
|'''Email:''' [mailto:lmccomm2@uiuc.edu ]<br />
|- <br />
|'''Home Town:''' Edwardsville, IL<br />
|-<br />
|'''Major:''' Molecular and Cellular Biology<br />
|-<br />
|'''Year:''' Senior<br />
|-<br />
|'''Research/Academic Interests:''' Synthetic Biology, dentistry<br />
|-<br />
|'''About Myself:''' I am very excited to be a part of the Illinois iGEM team this year! As a hobby I enjoy playing tennis.<br />
|}</div>Lmccomm2http://2009.igem.org/File:Illinois_lucy_pic.jpgFile:Illinois lucy pic.jpg2009-06-10T15:45:02Z<p>Lmccomm2: </p>
<hr />
<div></div>Lmccomm2http://2009.igem.org/User:Lmccomm2User:Lmccomm22009-06-10T15:40:44Z<p>Lmccomm2: New page: {{UIUC09Temp}} {{Illinois}} == Lucy McCommas == {|class="wikitable" border="0" width="800px" cellpadding="1" cellspacing="1" align="center" |- |width="400px"|'''Name:''' Lucy McCommas |...</p>
<hr />
<div>{{UIUC09Temp}}<br />
{{Illinois}}<br />
<br />
== Lucy McCommas ==<br />
<br />
{|class="wikitable" border="0" width="800px" cellpadding="1" cellspacing="1" align="center"<br />
|- <br />
|width="400px"|'''Name:''' Lucy McCommas<br />
|width="400px" rowspan="7" valign="top"|[[Image:]]<br />
|-<br />
|'''Email:''' [mailto:lmccomm2@uiuc.edu ]<br />
|- <br />
|'''Home Town:''' Edwardsville, IL<br />
|-<br />
|'''Major:''' Molecular and Cellular Biology<br />
|-<br />
|'''Year:''' Senior<br />
|-<br />
|'''Research/Academic Interests:''' Synthetic Biology, dentistry<br />
|-<br />
|'''About Myself:''' I am very excited to be a part of the Illinois iGEM team this year! As a hobby I enjoy playing tennis.<br />
|}</div>Lmccomm2http://2009.igem.org/Team:Illinois/TeamTeam:Illinois/Team2009-05-22T18:40:00Z<p>Lmccomm2: /* Meet the Team */</p>
<hr />
<div><!-- *** What falls between these lines is the Alert Box! You can remove it from your pages once you have read and understood the alert *** --><br />
<br />
<html><br />
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;"><br />
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;"><br />
This is a template page. READ THESE INSTRUCTIONS.<br />
</div><br />
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;"><br />
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.<br />
</div><br />
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;"><br />
You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook. PLEASE keep all of your pages within your teams namespace. <br />
</div><br />
</div><br />
</html><br />
<br />
<!-- *** End of the alert box *** --><br />
<br />
<br />
<br />
{|align="justify"<br />
|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.<br />
|[[Image:Example_logo.png|200px|right|frame]]<br />
|-<br />
|<br />
''Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)''<br />
|[[Image:Team.png|right|frame|Your team picture]]<br />
|-<br />
|<br />
|align="center"|[[Team:Illinois | Team Example 2]]<br />
|}<br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:Illinois|Home]]<br />
!align="center"|[[Team:Illinois/Team|The Team]]<br />
!align="center"|[[Team:Illinois/Project|The Project]]<br />
!align="center"|[[Team:Illinois/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:Illinois/Modeling|Modeling]]<br />
!align="center"|[[Team:Illinois/Notebook|Notebook]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
<br />
<br />
== '''Meet the Team''' ==<br />
{|border = "0"<br />
|-<br />
|rowspan="3" valign="top"|<br />
<br />
<br />
<br />
<br />
<br />
'''Advisors:'''<br />
<br />
*''' Advisor 1''': Mentor for all<br />
*'''Advisor 2''': Our favorite<br />
*'''Grad Student 1''': Our leader <br />
<br />
<br />
'''Undergrads:'''<br />
<br />
*[https://2009.igem.org/User:korench1 Dave Korenchan]<br />
*'''Student 2''': Math nerd<br />
*[https://2009.igem.org/User:reif David Reif]<br />
*'''Student 4''': Loves iGEM<br />
*[https://2009.igem.org/User:lmccomm2 Lucy McCommas] <br />
*[https://2009.igem.org/User:Graham Graham Heimberg]<br />
*'''Student 7''':<br />
*'''Student 8''': Sleepyhead <br />
*'''Student 9''': Math nerd<br />
*'''Student 10''': Is going to save the world<br />
*'''Student 11''': Loves iGEM<br />
*'''Student 12''': A normal student... or(r) am I?<br />
*'''Student 13''': Table football fan<br />
*'''Student 14''':<br />
<br />
<br />
|<br />
<gallery><br />
Image:Team_member_1.png|Team member 1<br />
Image:Team_member_2.png|Team member 2<br />
Image:Team_member_3.png|Team member 3<br />
Image:Team_member_4.png|Team member 4<br />
Image: Picture_igem.jpg<br />
Image:Team_member_6.png|Team member 6<br />
Image:Team_member_7.png|Team member 7<br />
Image:Team_member_8.png|Team member 8<br />
Image:Team_member_9.png|Team member 9<br />
Image:Team_member_10.png|Team member 10<br />
Image:Team_member_11.png|Team member 11<br />
Image:Team_member_12.png|Team member 12<br />
Image:Team_member_13.png|Team member 13<br />
Image:Team_member_14.png|Team member 14<br />
</gallery><br />
|}<br />
<br />
== '''What we did''' ==<br />
<br />
(Provide proper attribution for all work)<br />
<br />
<br />
== '''Where we're from''' ==</div>Lmccomm2http://2009.igem.org/Team:Illinois/TeamTeam:Illinois/Team2009-05-22T18:38:36Z<p>Lmccomm2: /* Meet the Team */</p>
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<div><!-- *** What falls between these lines is the Alert Box! You can remove it from your pages once you have read and understood the alert *** --><br />
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This is a template page. READ THESE INSTRUCTIONS.<br />
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;"><br />
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.<br />
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;"><br />
You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook. PLEASE keep all of your pages within your teams namespace. <br />
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{|align="justify"<br />
|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.<br />
|[[Image:Example_logo.png|200px|right|frame]]<br />
|-<br />
|<br />
''Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)''<br />
|[[Image:Team.png|right|frame|Your team picture]]<br />
|-<br />
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|align="center"|[[Team:Illinois | Team Example 2]]<br />
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:Illinois|Home]]<br />
!align="center"|[[Team:Illinois/Team|The Team]]<br />
!align="center"|[[Team:Illinois/Project|The Project]]<br />
!align="center"|[[Team:Illinois/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:Illinois/Modeling|Modeling]]<br />
!align="center"|[[Team:Illinois/Notebook|Notebook]]<br />
|}<br />
(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')<br />
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== '''Meet the Team''' ==<br />
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'''Advisors:'''<br />
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*''' Advisor 1''': Mentor for all<br />
*'''Advisor 2''': Our favorite<br />
*'''Grad Student 1''': Our leader <br />
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'''Undergrads:'''<br />
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*[https://2009.igem.org/User:korench1 Dave Korenchan]<br />
*'''Student 2''': Math nerd<br />
*[https://2009.igem.org/User:reif David Reif]<br />
*'''Student 4''': Loves iGEM<br />
*'''Student 5''': Lucy McCommas<br />
*[https://2009.igem.org/User:Graham Graham Heimberg]<br />
*'''Student 7''':<br />
*'''Student 8''': Sleepyhead <br />
*'''Student 9''': Math nerd<br />
*'''Student 10''': Is going to save the world<br />
*'''Student 11''': Loves iGEM<br />
*'''Student 12''': A normal student... or(r) am I?<br />
*'''Student 13''': Table football fan<br />
*'''Student 14''':<br />
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<gallery><br />
Image:Team_member_1.png|Team member 1<br />
Image:Team_member_2.png|Team member 2<br />
Image:Team_member_3.png|Team member 3<br />
Image:Team_member_4.png|Team member 4<br />
Image: Picture_igem.jpg<br />
Image:Team_member_6.png|Team member 6<br />
Image:Team_member_7.png|Team member 7<br />
Image:Team_member_8.png|Team member 8<br />
Image:Team_member_9.png|Team member 9<br />
Image:Team_member_10.png|Team member 10<br />
Image:Team_member_11.png|Team member 11<br />
Image:Team_member_12.png|Team member 12<br />
Image:Team_member_13.png|Team member 13<br />
Image:Team_member_14.png|Team member 14<br />
</gallery><br />
|}<br />
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== '''What we did''' ==<br />
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(Provide proper attribution for all work)<br />
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== '''Where we're from''' ==</div>Lmccomm2http://2009.igem.org/File:Picture_igem.jpgFile:Picture igem.jpg2009-05-22T18:37:17Z<p>Lmccomm2: </p>
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<div></div>Lmccomm2