http://2009.igem.org/wiki/index.php?title=Special:Contributions/OHoeller&feed=atom&limit=50&target=OHoeller&year=&month=2009.igem.org - User contributions [en]2024-03-28T23:08:33ZFrom 2009.igem.orgMediaWiki 1.16.5http://2009.igem.org/Team:UCSFTeam:UCSF2009-10-22T01:42:04Z<p>OHoeller: </p>
<hr />
<div><!--- The Mission, Experiments ---><br />
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<p align="center"><img src="https://static.igem.org/mediawiki/2009/7/7e/Wiki_2009CellBots.jpg" width="276" height="258" align="middle" /></p><br />
<br><br />
<br><br />
<p align="center" class="style2">Engineering Motile Cellular Robots</p><br />
<blockquote><br />
<br><br />
<h2 align="left">Abstract</h2><br />
<p align="left">Some eukaryotic cells, such as white blood cells, have the amazing ability to sense specific external chemical signals and move toward those signals. This behavior, known as chemotaxis, is a fundamental biological process crucial to such diverse functions as development, wound healing and immune response. In our project, we used a synthetic biology approach to manipulate signaling pathways that mediate chemotaxis in two model organisms:<br> HL-60 (neutrophil-like) cells and the slime mold, Dictyostelium discoideum. </p> <br />
<br />
<p align="left">In doing so, <strong>we have demonstrated that we can regulate both the navigation and speed of our cells, as well as harness their ability to carry a payload.</strong></p><br />
<br />
<p align="left">Through our manipulations, we hope to better understand how these systems work, and eventually to build or reprogram cells that can perform useful tasks. Imagine, for example, therapeutic nanorobots that could home to a directed site in the body and execute complex, user-defined functions (e.g., kill tumors, deliver drugs, guide stem cell migration and differentiation). Alternatively, imagine bioremediation nanorobots that could find and retrieve toxic substances. Such cellular robots could be revolutionary biotechnological tools.</p><br />
<p align="right"><a href="https://2009.igem.org/Team:UCSF/Project">More...</a></p><br />
<p align="right">&nbsp;</p><br />
<table width="870" border="0" cellpadding="3"><br />
<tr><br />
<td width="202" height="208"><img src="https://static.igem.org/mediawiki/2009/0/02/Wiki_2009project.jpg" alt="" width="240" height="224" /></td><br />
<td width="650"><h3>&nbsp;</h3><br />
<blockquote><br />
<h3>BUILDING CELL-BOTS</h3><br />
<blockquote><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Project">Introduction</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Navigation">Step 1 - Engineering NAVIGATION</a></h4><br />
<ul><br />
<ul><br />
<li><a href="https://2009.igem.org/Team:UCSF/Navigation">Inserting New Sensors</a></li><br />
<li><a href="https://2009.igem.org/Team:UCSF/NavigationPart2">Tuning Sensor Sensitivity</a></li><br />
</ul><br />
</ul><br />
<h4><a href="https://2009.igem.org/Team:UCSF/SPEED">Step 2 - Engineering SPEED</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/PAYLOAD">Step 3 - Carrying a PAYLOAD</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Future Applications">Our Vision for the Future</a></h4><br />
</blockquote><br />
</blockquote><br />
<p>&nbsp;</p></td><br />
</tr><br />
</table><br />
<table width="870" border="0" cellpadding="3"><br />
<tr><br />
<td width="202" height="208"><img src="https://static.igem.org/mediawiki/2009/d/db/Wiki_2009Team.jpg" alt="" width="240" height="224" /></td><br />
<td width="650"><h3>&nbsp;</h3><br />
<blockquote><br />
<h3>OUR TEAM</h3><br />
<blockquote><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Team">Team Members</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Notebook">Notebooks</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Our_summer_experience">Summer Experience</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Human Practices">Human Practices</a></h4><br />
<h4><a href="http://dspace.mit.edu/handle/1721.1/46721">NEW BIOBRICK Standard RFC28 - Aar1 Cloning System</a></h4><br />
<h4><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=UCSF">Parts submitted to the Registry</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Gold Medal Requisites">GOLD MEDAL Requisites</a></h4><br />
</blockquote><br />
</blockquote><br />
<p>&nbsp;</p></td><br />
</tr><br />
</table><br />
<br><br></br></br><br />
</blockquote><br />
</body><br />
</html><br />
<br />
<br />
'''UCSF iGEM 2009 is sponsored by...'''<br />
<br />
<br />
<br />
<html><br />
<p align="center"><img src="https://static.igem.org/mediawiki/2009/8/85/Wiki_2009Sponsors.jpg" width="690" height="419" align="middle" /></p></html></div>OHoellerhttp://2009.igem.org/Team:UCSFTeam:UCSF2009-10-22T01:39:46Z<p>OHoeller: </p>
<hr />
<div><!--- The Mission, Experiments ---><br />
<br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" /><br />
<title>Untitled Document</title><br />
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<body><br />
<p align="center"><img src="https://static.igem.org/mediawiki/2009/7/7e/Wiki_2009CellBots.jpg" width="276" height="258" align="middle" /></p><br />
<p align="center" class="style2">Engineering Motile Cellular Robots</p><br />
<blockquote><br />
<br><br />
<h2 align="left">Abstract</h2><br />
<p align="left">Some eukaryotic cells, such as white blood cells, have the amazing ability to sense specific external chemical signals and move toward those signals. This behavior, known as chemotaxis, is a fundamental biological process crucial to such diverse functions as development, wound healing and immune response. In our project, we used a synthetic biology approach to manipulate signaling pathways that mediate chemotaxis in two model organisms:<br> HL-60 (neutrophil-like) cells and the slime mold, Dictyostelium discoideum. </p> <br />
<br />
<p align="left">In doing so, <strong>we have demonstrated that we can regulate both the navigation and speed of our cells, as well as harness their ability to carry a payload.</strong></p><br />
<br />
<p align="left">Through our manipulations, we hope to better understand how these systems work, and eventually to build or reprogram cells that can perform useful tasks. Imagine, for example, therapeutic nanorobots that could home to a directed site in the body and execute complex, user-defined functions (e.g., kill tumors, deliver drugs, guide stem cell migration and differentiation). Alternatively, imagine bioremediation nanorobots that could find and retrieve toxic substances. Such cellular robots could be revolutionary biotechnological tools.</p><br />
<p align="right"><a href="https://2009.igem.org/Team:UCSF/Project">More...</a></p><br />
<p align="right">&nbsp;</p><br />
<table width="870" border="0" cellpadding="3"><br />
<tr><br />
<td width="202" height="208"><img src="https://static.igem.org/mediawiki/2009/0/02/Wiki_2009project.jpg" alt="" width="240" height="224" /></td><br />
<td width="650"><h3>&nbsp;</h3><br />
<blockquote><br />
<h3>BUILDING CELL-BOTS</h3><br />
<blockquote><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Project">Introduction</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Navigation">Step 1 - Engineering NAVIGATION</a></h4><br />
<ul><br />
<ul><br />
<li><a href="https://2009.igem.org/Team:UCSF/Navigation">Inserting New Sensors</a></li><br />
<li><a href="https://2009.igem.org/Team:UCSF/NavigationPart2">Tuning Sensor Sensitivity</a></li><br />
</ul><br />
</ul><br />
<h4><a href="https://2009.igem.org/Team:UCSF/SPEED">Step 2 - Engineering SPEED</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/PAYLOAD">Step 3 - Carrying a PAYLOAD</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Future Applications">Our Vision for the Future</a></h4><br />
</blockquote><br />
</blockquote><br />
<p>&nbsp;</p></td><br />
</tr><br />
</table><br />
<table width="870" border="0" cellpadding="3"><br />
<tr><br />
<td width="202" height="208"><img src="https://static.igem.org/mediawiki/2009/d/db/Wiki_2009Team.jpg" alt="" width="240" height="224" /></td><br />
<td width="650"><h3>&nbsp;</h3><br />
<blockquote><br />
<h3>OUR TEAM</h3><br />
<blockquote><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Team">Team Members</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Notebook">Notebooks</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Our_summer_experience">Summer Experience</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Human Practices">Human Practices</a></h4><br />
<h4><a href="http://dspace.mit.edu/handle/1721.1/46721">NEW BIOBRICK Standard RFC28 - Aar1 Cloning System</a></h4><br />
<h4><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=UCSF">Parts submitted to the Registry</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Gold Medal Requisites">GOLD MEDAL Requisites</a></h4><br />
</blockquote><br />
</blockquote><br />
<p>&nbsp;</p></td><br />
</tr><br />
</table><br />
<br><br></br></br><br />
</blockquote><br />
</body><br />
</html><br />
<br />
<br />
'''UCSF iGEM 2009 is sponsored by...'''<br />
<br />
<br />
<br />
<html><br />
<p align="center"><img src="https://static.igem.org/mediawiki/2009/8/85/Wiki_2009Sponsors.jpg" width="690" height="419" align="middle" /></p></html></div>OHoellerhttp://2009.igem.org/Team:UCSF/Our_summer_experienceTeam:UCSF/Our summer experience2009-10-22T01:31:32Z<p>OHoeller: </p>
<hr />
<div>== '''Summer Curriculum''' ==<br />
* '''[[Selecting/Development of the iGEM team]]''': High School teacher Mr.Cachianes hand selected seven students from his second year biotechnology class for the iGEM team.<br />
* '''[[Pre-meetings]]''': Starting early April, Mr.Cachianes and his seven students met with UCSF's iGEM coordinators to start the pre-project and learn more about synthetic biology and cell motility.<br />
* '''[[Bootcamp]]''': For two weeks, the iGEM team met everyday for four hour lectures on the mechanisms of cell motility, and discussed scientific papers.<br />
* '''[[Student Brainstorming]]''': Bootcamp culminated in one large brainstorming project where we put our heads together and put what we learned to the test by trying to find ways to brake, accelerate, and steer a cell. <br />
* '''[[Execution]]''': We took the best ideas from brainstorming, modified them, and prepared to carry them out.<br />
*'''[[Curriculum for Teachers]]''' by Saber Khan: Cells as Robots Unit: iGEM/UCSF Summer 2009<br />
<br />
== '''Personal Blogs about the Experience''' ==<br />
* [http://arsigem.blogspot.com/ '''Alex Smith''']<br />
* [http://caiallen.tumblr.com/ '''Allen Cai''']: iGEM gave me hands-on experience on how it would be like to work in a real lab. I loved it and couldn't see myself doing anything else this summer than iGEM.<br />
* [http://cathyliu.tumblr.com/ '''Cathy Liu''']: This summer has been a lot of things. Demanding, stressful, tedious? Sure. But it has definitely been amazing, and a bunch of things I couldn’t express with words. I know we’re going to rock the Jamboree; it’s going to be epic. And I know I’ll only think of all the wonderful times when looking back at all this. There will always be a special place in my heart for UCSF. (P.S. “SUCCESS!”)<br />
* [http://miaomiao.tumblr.com/ '''Edna Miao''']: iGEM has forced me outside my comfort zone many times and consequently has taught me better public speaking and critical thinking skills, on top of new lab techniques of course.<br />
* [http://echan08.blogspot.com/ '''Ethan Chan''']: This experience has brought me closer to my peers and gave me critical lab experience that not many High School students get! <br />
* [http://ertwong.tumblr.com/ '''Eric Wong''']: MADNESS? THIS IS RESEARCH!!! >:O well yeah i enjoyed my time at UCSF, I learned many new things such as lab techniques and chemotaxis as well as meet a bunch of people who i got along great with. CAZZO!!!<br />
* '''Hansi Liu'''<br />
* [http://tackiejam.tumblr.com/ '''Jackie Tam''']: Participating on UCSF’s iGEM team is unparalleled learning experience. This is my first formal lab experience, and I am incredibly thankful that it took place here at the Lim Lab at UCSF.<br />
* '''Katja Kolar''': Mix half a gallon of chemotaxis, mid-sized pack of surface tension gels, big handful of amazing UCSF people, a cup of interesting science, and sprinkle with curious and fun iGEM kids. Bake for 3 months in San Francisco, serve anytime! I loved being part of this summer experience.<br />
* [http://ryanquan.tumblr.com/ '''Ryan Quan''']: This summer I learned many new techniques and how cells move, but the best thing was teaching other people techniques because it really makes you make sure you know your stuff before you open you explain something. <br />
* [http://ryeland.tumblr.com/ '''Ryan Liang''']: This summer has been engagingly educational and highly demanding as well as personally valuable in career-building.<br />
<br />
* [http://davidpincus.tumblr.com/ '''Buddy David Pincus's blog''']<br />
<br />
<br />
<br />
{| style="color:#333333;background-color:#cccccc;" cellpadding="3" cellspacing="3" border="0" bordercolor="#231f26" width="99%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}</div>OHoellerhttp://2009.igem.org/Team:UCSF/Our_summer_experienceTeam:UCSF/Our summer experience2009-10-22T01:31:11Z<p>OHoeller: </p>
<hr />
<div>== '''Summer Curriculum''' ==<br />
* '''[[Selecting/Development of the iGEM team]]''': High School teacher Mr.Cachianes hand selected seven students from his second year biotechnology class for the iGEM team.<br />
* '''[[Pre-meetings]]''': Starting early April, Mr.Cachianes and his seven students met with UCSF's iGEM coordinators to start the pre-project and learn more about synthetic biology and cell motility.<br />
* '''[[Bootcamp]]''': For two weeks, the iGEM team met everyday for four hour lectures on the mechanisms of cell motility, and discussed scientific papers.<br />
* '''[[Student Brainstorming]]''': Bootcamp culminated in one large brainstorming project where we put our heads together and put what we learned to the test by trying to find ways to brake, accelerate, and steer a cell. <br />
* '''[[Execution]]''':We took the best ideas from brainstorming, modified them, and prepared to carry them out.<br />
*'''[[Curriculum for Teachers]]''' by Saber Khan: Cells as Robots Unit: iGEM/UCSF Summer 2009<br />
<br />
== '''Personal Blogs about the Experience''' ==<br />
* [http://arsigem.blogspot.com/ '''Alex Smith''']<br />
* [http://caiallen.tumblr.com/ '''Allen Cai''']: iGEM gave me hands-on experience on how it would be like to work in a real lab. I loved it and couldn't see myself doing anything else this summer than iGEM.<br />
* [http://cathyliu.tumblr.com/ '''Cathy Liu''']: This summer has been a lot of things. Demanding, stressful, tedious? Sure. But it has definitely been amazing, and a bunch of things I couldn’t express with words. I know we’re going to rock the Jamboree; it’s going to be epic. And I know I’ll only think of all the wonderful times when looking back at all this. There will always be a special place in my heart for UCSF. (P.S. “SUCCESS!”)<br />
* [http://miaomiao.tumblr.com/ '''Edna Miao''']: iGEM has forced me outside my comfort zone many times and consequently has taught me better public speaking and critical thinking skills, on top of new lab techniques of course.<br />
* [http://echan08.blogspot.com/ '''Ethan Chan''']: This experience has brought me closer to my peers and gave me critical lab experience that not many High School students get! <br />
* [http://ertwong.tumblr.com/ '''Eric Wong''']: MADNESS? THIS IS RESEARCH!!! >:O well yeah i enjoyed my time at UCSF, I learned many new things such as lab techniques and chemotaxis as well as meet a bunch of people who i got along great with. CAZZO!!!<br />
* '''Hansi Liu'''<br />
* [http://tackiejam.tumblr.com/ '''Jackie Tam''']: Participating on UCSF’s iGEM team is unparalleled learning experience. This is my first formal lab experience, and I am incredibly thankful that it took place here at the Lim Lab at UCSF.<br />
* '''Katja Kolar''': Mix half a gallon of chemotaxis, mid-sized pack of surface tension gels, big handful of amazing UCSF people, a cup of interesting science, and sprinkle with curious and fun iGEM kids. Bake for 3 months in San Francisco, serve anytime! I loved being part of this summer experience.<br />
* [http://ryanquan.tumblr.com/ '''Ryan Quan''']: This summer I learned many new techniques and how cells move, but the best thing was teaching other people techniques because it really makes you make sure you know your stuff before you open you explain something. <br />
* [http://ryeland.tumblr.com/ '''Ryan Liang''']: This summer has been engagingly educational and highly demanding as well as personally valuable in career-building.<br />
<br />
* [http://davidpincus.tumblr.com/ '''Buddy David Pincus's blog''']<br />
<br />
<br />
<br />
{| style="color:#333333;background-color:#cccccc;" cellpadding="3" cellspacing="3" border="0" bordercolor="#231f26" width="99%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
|}</div>OHoellerhttp://2009.igem.org/Team:UCSFTeam:UCSF2009-10-22T01:29:08Z<p>OHoeller: </p>
<hr />
<div><!--- The Mission, Experiments ---><br />
<br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" /><br />
<title>Untitled Document</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin-left: 20px;<br />
margin-right: 20px;<br />
width: 900px;<br />
}<br />
.style2 {<br />
font-size: 30px;<br />
font-weight: bold;<br />
}<br />
--><br />
</style></head><br />
<body><br />
<p align="center"><img src="https://static.igem.org/mediawiki/2009/7/7e/Wiki_2009CellBots.jpg" width="276" height="258" align="middle" /></p><br />
<p align="center" class="style2">Engineering Motility of Cellular Robots</p><br />
<blockquote><br />
<br><br />
<h2 align="left">Abstract</h2><br />
<p align="left">Some eukaryotic cells, such as white blood cells, have the amazing ability to sense specific external chemical signals and move toward those signals. This behavior, known as chemotaxis, is a fundamental biological process crucial to such diverse functions as development, wound healing and immune response. In our project, we used a synthetic biology approach to manipulate signaling pathways that mediate chemotaxis in two model organisms:<br> HL-60 (neutrophil-like) cells and the slime mold, Dictyostelium discoideum. </p> <br />
<br />
<p align="left">In doing so, <strong>we have demonstrated that we can regulate both the navigation and speed of our cells, as well as harness their ability to carry a payload.</strong></p><br />
<br />
<p align="left">Through our manipulations, we hope to better understand how these systems work, and eventually to build or reprogram cells that can perform useful tasks. Imagine, for example, therapeutic nanorobots that could home to a directed site in the body and execute complex, user-defined functions (e.g., kill tumors, deliver drugs, guide stem cell migration and differentiation). Alternatively, imagine bioremediation nanorobots that could find and retrieve toxic substances. Such cellular robots could be revolutionary biotechnological tools.</p><br />
<p align="right"><a href="https://2009.igem.org/Team:UCSF/Project">More...</a></p><br />
<p align="right">&nbsp;</p><br />
<table width="870" border="0" cellpadding="3"><br />
<tr><br />
<td width="202" height="208"><img src="https://static.igem.org/mediawiki/2009/0/02/Wiki_2009project.jpg" alt="" width="240" height="224" /></td><br />
<td width="650"><h3>&nbsp;</h3><br />
<blockquote><br />
<h3>BUILDING CELL-BOTS</h3><br />
<blockquote><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Project">Introduction</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Navigation">Step 1 - Engineering NAVIGATION</a></h4><br />
<ul><br />
<ul><br />
<li><a href="https://2009.igem.org/Team:UCSF/Navigation">Inserting New Sensors</a></li><br />
<li><a href="https://2009.igem.org/Team:UCSF/NavigationPart2">Tuning Sensor Sensitivity</a></li><br />
</ul><br />
</ul><br />
<h4><a href="https://2009.igem.org/Team:UCSF/SPEED">Step 2 - Engineering SPEED</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/PAYLOAD">Step 3 - Carrying a PAYLOAD</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Future Applications">Our Vision for the Future</a></h4><br />
</blockquote><br />
</blockquote><br />
<p>&nbsp;</p></td><br />
</tr><br />
</table><br />
<table width="870" border="0" cellpadding="3"><br />
<tr><br />
<td width="202" height="208"><img src="https://static.igem.org/mediawiki/2009/d/db/Wiki_2009Team.jpg" alt="" width="240" height="224" /></td><br />
<td width="650"><h3>&nbsp;</h3><br />
<blockquote><br />
<h3>OUR TEAM</h3><br />
<blockquote><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Team">Team Members</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Notebook">Notebooks</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Our_summer_experience">Summer Experience</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Human Practices">Human Practices</a></h4><br />
<h4><a href="http://dspace.mit.edu/handle/1721.1/46721">NEW BIOBRICK Standard RFC28 - Aar1 Cloning System</a></h4><br />
<h4><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=UCSF">Parts submitted to the Registry</a></h4><br />
<h4><a href="https://2009.igem.org/Team:UCSF/Gold Medal Requisites">GOLD MEDAL Requisites</a></h4><br />
</blockquote><br />
</blockquote><br />
<p>&nbsp;</p></td><br />
</tr><br />
</table><br />
<br><br></br></br><br />
</blockquote><br />
</body><br />
</html><br />
<br />
<br />
'''UCSF iGEM 2009 is sponsored by...'''<br />
<br />
<br />
<br />
<html><br />
<p align="center"><img src="https://static.igem.org/mediawiki/2009/8/85/Wiki_2009Sponsors.jpg" width="690" height="419" align="middle" /></p></html></div>OHoellerhttp://2009.igem.org/Team:UCSF/NotebookTeam:UCSF/Notebook2009-10-22T01:24:04Z<p>OHoeller: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" /><br />
<title>Untitled Document</title><br />
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<p align="left">In the beginning of the summer our team was divided into two groups - one group focused on Engineering NAVIGATION and the other group focused on Engineering SPEED. The group that engineered NAVIGATION worked with HL60 cells - HL60 Team. The group that engineered SPEED worked with <em>Dictyostelium</em> - Dicty Team.</p><br />
<blockquote><br />
<h2 align="left">HL60 Team</h2><br />
<p align="left"><strong>GOAL</strong>: Our goals for this project are to screen 30 various different GPCR's (G couple protein receptors) to determine which receptors mediate chemotaxis using the high through put transwell assay. After the first phase screening using 5-6 day differentiated HL-60 cell transfected with known receptors is completed, we move on to the secondary phase of screening with viable chemotatic receptors. During the second phase of screening we fused &quot;Actin Modulators&quot;, &quot;PIP3 Modulators&quot;, and &quot;GEF Activators&quot; in hopes of an accelerated chemotatic response. </p><br />
<p align="left">&nbsp;</p><br />
<p align="left"> <strong>ENGINEERING NAVIGATION</strong>:</p><br />
<ul><br />
<li><em>Can we send our cells to new targets?</em> Inserting new Sensors to HL60 (GPCR Screening)</li><br />
<li><em>Can we make our new engineered cells more or less sensitive to the new signals?</em> Tuning receptor sensitivity by attaching recycling modules to the new GPCRs</li><br />
</ul><br />
<p><strong>Milestones</strong>:</p><br />
<p><strong>Step 1.</strong> Clone receptors, N-terminal tags, C-terminal domains. Generate fusions. (<strong>everyone</strong>)<br /><br />
<strong>Step 2.</strong> Transwell screen to identify constructs that affect migration (<strong>Ryan, Eric</strong>)<br /><br />
<strong>Step 3.</strong> Time lapse microscopy to determine directional migration (<strong>Hansi, Jackie, Katja</strong>)<br /><br />
<strong>Step 4.</strong> Establish method for attaching beads to cells (<strong>Katja</strong>)<br /><br />
<p><strong>Team members</strong></p><br />
<p><br />
<a href="https://2009.igem.org/Eric_Wong's_notebook">Eric Wong's notebook</a>: I worked on cloning, PCR, Gel purification and extraction, and was apart of the GPCR screening team.<br /><br />
<a href="https://2009.igem.org/Jackie_Tam's_notebook">Jackie Tam's notebook</a>: I was responsible for cloning, microscopy, and video analysis.<br /><br />
<a href="https://2009.igem.org/Ryan_Liang's_notebook">Ryan Liang's notebook</a>: I cloned parts to be used in the GPCR screens, transfected HL-60 cells with various different GPCRs, ran transwell assays, and analyzed/compared transwell assay results.<br /><br />
<a href="https://2009.igem.org/Hansi_Liu's_notebook">Hansi Liu's notebook</a>: I established a protocol for time-lapse microscopy of HL-60 cells crawling in gradients, and collected that data.</p><br />
<p>&nbsp;</p><br />
<p align="left"><strong>MAKING CELLS CARRY PAYLOAD</strong>:</p><br />
<ul><br />
<li><em>Can we make our cells carry stuff?</em> - proof-of-concept: attach beads to cells </li><br />
</ul><br />
<p><strong>Team members</strong></p><br />
<p><a href="https://2009.igem.org/Katja_Kolar's_notebook">Katja Kolar's notebook</a>: I did microscopy with wt and hM4D-transfected HL-60 cells, experiments for the &quot;Payload&quot; part of the project, and cloned HL-60 team constructs. </p><br />
<p align="left">&nbsp;</p><br />
<h2 align="left">Dicty Team</h2><br />
<p align="left"><strong>GOAL</strong>: Our goal was to build brakes and accelerators for motile cell. We challenged ourselves to engineer feedback loops that would affect the localization of PIP3 within the cell. </p><br />
<p align="left">&nbsp;</p><br />
<p align="left"> <strong>ENGINEERING SPEED</strong>:</p><br />
<ul><br />
<li><em>Can we send our cells move faster or slower?</em> Creating brakes and accelerators by modulating PIP3 polarity - building synthetic feedback loops by fusing catalytic and localization domains.</li><br />
</ul><br />
<p><strong>Milestones</strong>:</p><br />
<p><strong>Step 1.</strong> PCR parts: catalytic and localization domains <strong>Ryan</strong><br /><br />
<strong>Step 2.</strong> Create combinations of localization domains and catalytic domains (feedback elements) <strong>everyone</strong><br /><br />
<strong>Step 3.</strong> Generate Dictyostelium strains expressing feedback elements <strong>everyone</strong><br /><br />
<strong>Step 4.</strong> Measure motility parameters (speed, directionality) <strong>everyone</strong><br /><br />
<strong>Step 5.</strong> Analyze data. Create histograms and compare if our new feedback elements had any effect on Dicty. Do they cause them to move faster or slower? <strong>everyone</strong></p><br />
<p><strong>Team members</strong></p><br />
<p> <a href="https://2009.igem.org/Allen_Cai's_notebook">Allen Cai's notebook</a>: I worked mostly on the cloning part of the project. I ligated a lot of the localization and catalytic domains together. I also took care of some dicty strains and made movies of them.<br /><br />
<a href="https://2009.igem.org/Alex_Smith's_notebook">Alex Smith's notebook</a>:<br /><br />
<a href="https://2009.igem.org/Edna_Miao's_notebook">Edna Miao's notebook</a>: I transformed new constructs into Dicty, analyzed data, and worked with various different Dicty strains.<br /><br />
<a href="https://2009.igem.org/Ethan_Chan's_notebook">Ethan Chan's notebook</a>: I constructed Dicty cells with new feedback elements, analyzed data, and worked with various different Dicty strains.<br /><br />
<a href="https://2009.igem.org/Ryan_Quan's_notebook">Ryan Quan's notebook</a>: I worked on team dicty and taught the students some basic cloning techniques but mainly learned many new things along with them. </p><br />
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{| style="color:#333333;background-color:#cccccc;" cellpadding="3" cellspacing="3" border="0" bordercolor="#231f26" width="99%" align="center"<br />
!align="center"|[[Team:UCSF|Home]]<br />
!align="center"|[[Team:UCSF/Team|The Team]]<br />
!align="center"|[[Team:UCSF/Project|The Project]]<br />
!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:UCSF/Our summer experience|Our summer experience]]<br />
!align="center"|[[Team:UCSF/Notebook|Notebook]]<br />
!align="center"|[[Team:UCSF/Human Practices|Human Practices]]<br />
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