http://2009.igem.org/wiki/index.php?title=Special:Contributions/Pony&feed=atom&limit=50&target=Pony&year=&month=2009.igem.org - User contributions [en]2024-03-28T09:20:08ZFrom 2009.igem.orgMediaWiki 1.16.5http://2009.igem.org/Team:SJTU-BioX-Shanghai/The_teamTeam:SJTU-BioX-Shanghai/The team2011-07-07T06:12:06Z<p>Pony: </p>
<hr />
<div>{{Template:SJTU09_header}}<br />
{{Template:SJTU09_menubar}}<br />
=Team introduction=<br />
This is our first time to attend iGEM competition~ We're SJTUers and we love it!<br />
[[Image:SJTU09_Logo.jpg|center|thumb|400px|Team Logo]]<br />
<br />
==Team story==<br />
It is for the first time that Shanghai Jiaotong University(SJTU) participated in the iGEM competition held by MIT. At first, only a few students heard about such a competition. However, the news was spread so quickly among students that a team representing our university was formed in no time. <br />
<br />
[[Image:SJTU09 Album Members3.jpg|center|thumb|499px|Many members took part in our team to work together at the first period.]]<br />
<br />
In the early period, we have nothing but the passion of the team members, no instructions, laboratory or experimental funding. It was a tough time for us. We browsed the website of iGEM in order to learn more about the competition while writing letters to professors and executives for support. One day, in our excitement, we found a letter from the Academician He from the BioX-Center. He showed great interest in the competition and asked for more detailed information of it. He was so busy at that time that we had a talk with him in his car, since he rushed to an important meeting after that. We described the international competition to him in great detail. So interested was he that he agreed to extend support to us and contact the principal and secretary of our school to gain funding support from school. Thus, the SJTU Bio-X iGEM team was successfully established.<br />
<br />
[[Image:SJTU09 Album Lab1.jpg|center|thumb|499px|Bio-X Center provided us a lab to work in.]]<br />
<br />
After the team establishment, we have absorbed students from all kinds of department throughout the school, bioscience, math, electrical engineering and so on. We began to brainstorm our project while learning more knowledge about the biosynthesizing field. We came up with a lot of interesting ideas, such as yeast that can produce strawberry bread, a bacterial gameboy, a biological monitor that can realize the transformation of pictures by fluorescent protein. Though some of ideas appear to be intriguing but are actually hard to realize. We were fond of thinking freely without any constraint. We guess it may also be the essence of the competition.<br />
<br />
[[Image:SJTU09 Album Meeting2.jpg|center|thumb|499px|Our school's library offered us a meeting room.]]<br />
<br />
Since it is the first time, we came across a lot of difficulties in the process because of lack of experience, such as having no idea about parts and standardized assembly. All the work before the Workshop has been put in vain.<br />
<br />
[[Image:SJTU09 Album London.jpg|center|thumb|499px|Our representatives attended the iGEM Workshop in London.]]<br />
<br />
In the summer, we encountered the biggest obstacle that we have ever met. Since we failed to plan all things well, students have already participated in a lot of lab internship programs and there were few students left in the lab. The initial plan of competing with two projects has put a lot of pressure on us. Fortunately, we invited the team from Peking University to have a discussion and share the experience with us at the end of July. After that, we finally found the crux of the problem. We recruited our members at the beginning of August and disciplined the team. At the same time, we made up our mind to focus on a single project by choosing from the two. The meeting has become a turning point of the team.<br />
<br />
[[Image:SJTU09 collaboration PKU1.jpg|center|thumb|499px|[[Team:PKU_Beijing|PKU_Beijing Team]]'s visit to our school.]]<br />
<br />
Everything is going well after that. We combined our effort in doing our lab task, discussing wiki, designing our post and preparing out presentation. In the end, we are proud to stand on the podium of the MIT for 2009 iGEM competition and show our fruitful result to the whole world.<br />
<br />
[[Image:SJTU09 Album Materials.jpg|center|thumb|499px|Our original design of ur wiki frame, cultural shirt & mascots ]]<br />
<br />
When looking back into the team formation process, we found that we were lucky. Though we have felt our way out and went through numerous tough moments, we finally make it. We hold our firm belief in no pain, no gain. The result may not be the medal, but rather the experience itself. <br />
<br />
<br />
<br />
----<br />
<html><br />
<script><br />
function addFavorite(siteUrl, siteName)<br />
{<br />
if (document.all)window.external.addFavorite(siteUrl, siteName);<br />
else if (window.sidebar)window.sidebar.addPanel(siteName, siteUrl, "");<br />
}<br />
</script><br />
<ul><br />
<li><a href="##" onclick="addFavorite('https://2009.igem.org/Team:SJTU-BioX-Shanghai','Team SJTU-BioX-Shanghai#bannerTop')">Add to Favorites<img src="https://static.igem.org/mediawiki/2009/f/f1/Ensarija_Star_%28soft_edges%29.png"></a></li><br />
<li><a href="mailto:kermit_tao@163.com?subject=Greetings%20from%20iGEM">Contact us</a></li><br />
</ul><br />
</html><br />
Go to see [[Team:SJTU-BioX-Shanghai/Members#bannerTop|Members]].<br />
{{Template:SJTU09_enddiv}}<br />
{{Template:SJTU09_enddiv}}</div>Ponyhttp://2009.igem.org/Team:SJTU-BioX-Shanghai/The_teamTeam:SJTU-BioX-Shanghai/The team2011-07-07T06:11:37Z<p>Pony: </p>
<hr />
<div>{{Template:SJTU09_header}}<br />
{{Template:SJTU09_menubar}}<br />
=ttttttttt=<br />
=Team introduction=<br />
This is our first time to attend iGEM competition~ We're SJTUers and we love it!<br />
[[Image:SJTU09_Logo.jpg|center|thumb|400px|Team Logo]]<br />
<br />
==Team story==<br />
It is for the first time that Shanghai Jiaotong University(SJTU) participated in the iGEM competition held by MIT. At first, only a few students heard about such a competition. However, the news was spread so quickly among students that a team representing our university was formed in no time. <br />
<br />
[[Image:SJTU09 Album Members3.jpg|center|thumb|499px|Many members took part in our team to work together at the first period.]]<br />
<br />
In the early period, we have nothing but the passion of the team members, no instructions, laboratory or experimental funding. It was a tough time for us. We browsed the website of iGEM in order to learn more about the competition while writing letters to professors and executives for support. One day, in our excitement, we found a letter from the Academician He from the BioX-Center. He showed great interest in the competition and asked for more detailed information of it. He was so busy at that time that we had a talk with him in his car, since he rushed to an important meeting after that. We described the international competition to him in great detail. So interested was he that he agreed to extend support to us and contact the principal and secretary of our school to gain funding support from school. Thus, the SJTU Bio-X iGEM team was successfully established.<br />
<br />
[[Image:SJTU09 Album Lab1.jpg|center|thumb|499px|Bio-X Center provided us a lab to work in.]]<br />
<br />
After the team establishment, we have absorbed students from all kinds of department throughout the school, bioscience, math, electrical engineering and so on. We began to brainstorm our project while learning more knowledge about the biosynthesizing field. We came up with a lot of interesting ideas, such as yeast that can produce strawberry bread, a bacterial gameboy, a biological monitor that can realize the transformation of pictures by fluorescent protein. Though some of ideas appear to be intriguing but are actually hard to realize. We were fond of thinking freely without any constraint. We guess it may also be the essence of the competition.<br />
<br />
[[Image:SJTU09 Album Meeting2.jpg|center|thumb|499px|Our school's library offered us a meeting room.]]<br />
<br />
Since it is the first time, we came across a lot of difficulties in the process because of lack of experience, such as having no idea about parts and standardized assembly. All the work before the Workshop has been put in vain.<br />
<br />
[[Image:SJTU09 Album London.jpg|center|thumb|499px|Our representatives attended the iGEM Workshop in London.]]<br />
<br />
In the summer, we encountered the biggest obstacle that we have ever met. Since we failed to plan all things well, students have already participated in a lot of lab internship programs and there were few students left in the lab. The initial plan of competing with two projects has put a lot of pressure on us. Fortunately, we invited the team from Peking University to have a discussion and share the experience with us at the end of July. After that, we finally found the crux of the problem. We recruited our members at the beginning of August and disciplined the team. At the same time, we made up our mind to focus on a single project by choosing from the two. The meeting has become a turning point of the team.<br />
<br />
[[Image:SJTU09 collaboration PKU1.jpg|center|thumb|499px|[[Team:PKU_Beijing|PKU_Beijing Team]]'s visit to our school.]]<br />
<br />
Everything is going well after that. We combined our effort in doing our lab task, discussing wiki, designing our post and preparing out presentation. In the end, we are proud to stand on the podium of the MIT for 2009 iGEM competition and show our fruitful result to the whole world.<br />
<br />
[[Image:SJTU09 Album Materials.jpg|center|thumb|499px|Our original design of ur wiki frame, cultural shirt & mascots ]]<br />
<br />
When looking back into the team formation process, we found that we were lucky. Though we have felt our way out and went through numerous tough moments, we finally make it. We hold our firm belief in no pain, no gain. The result may not be the medal, but rather the experience itself. <br />
<br />
<br />
<br />
----<br />
<html><br />
<script><br />
function addFavorite(siteUrl, siteName)<br />
{<br />
if (document.all)window.external.addFavorite(siteUrl, siteName);<br />
else if (window.sidebar)window.sidebar.addPanel(siteName, siteUrl, "");<br />
}<br />
</script><br />
<ul><br />
<li><a href="##" onclick="addFavorite('https://2009.igem.org/Team:SJTU-BioX-Shanghai','Team SJTU-BioX-Shanghai#bannerTop')">Add to Favorites<img src="https://static.igem.org/mediawiki/2009/f/f1/Ensarija_Star_%28soft_edges%29.png"></a></li><br />
<li><a href="mailto:kermit_tao@163.com?subject=Greetings%20from%20iGEM">Contact us</a></li><br />
</ul><br />
</html><br />
Go to see [[Team:SJTU-BioX-Shanghai/Members#bannerTop|Members]].<br />
{{Template:SJTU09_enddiv}}<br />
{{Template:SJTU09_enddiv}}</div>Ponyhttp://2009.igem.org/Team:Heidelberg/PartsTeam:Heidelberg/Parts2011-07-04T09:06:48Z<p>Pony: /* Parts */</p>
<hr />
<div>{{Template_HD_3}}<br />
<html><body id="parts"></body></html><br />
{|<br />
|-valign="top" border="0" style="margin-left: 2px;"<br />
|width="650px" style="padding: 0 15px 15px 20px; background-color:#ede8e2"|<br />
__NOTOC__<br />
= Parts =<br />
== Measurement Kit ==<br />
[[Team:Heidelberg/Project_Measurement|''Main Article: Measurement'']]<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | pSMB_MEASURE: Promoter Measurement plasmid (mammalian) || Plasmid backbone || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" |pSMB_REFERENCE || Plasmid || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203099 Part:BBa_K203099]<br />
|-<br />
|}<br />
<br />
== Core promoters ==<br />
<br />
[[Team:Heidelberg/Project_Measurement#Different_core_promoters_result_in_different_expression_strength|''Main Article: Core promoters'']]<br />
<br />
The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by [[Project_Measurement#Different_core_promoters_result_in_different_expression_strength|core promoter swapping]].<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | JeT || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | JeT proximal-CMV core || Regulatory || [http://partsregistry.org/Part:BBa_K203113 Part:BBa_K203113]<br />
|-<br />
|}<br />
<br />
== Promoters created by RA-PCR ==<br />
<br />
[[Team:Heidelberg/Project_Synthetic_promoters|''Main article: Synthetic promoters'']]<br />
<br />
All contain the core promoter of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112] and were screened / measured in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100].<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Constitutive promoter; 0.4 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203110 Part:BBa_K203110]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Constitutive promoter; 1 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203118 Part:BBa_K203118]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Constitutive promoter; 2 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203111 Part:BBa_K203111]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Constitutive promoter; 2.5 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203109 Part:BBa_K203109]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | NF-&kappa;B regulated promoter || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203119 Part:BBa_K203119]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | pPAR&gamma; regulated promoter 1 || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203114 Part:BBa_K203114]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | pPAR&gamma; regulated promoter 2 || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203115 Part:BBa_K203115]<br />
|-<br />
|}<br />
<br />
== HEARTBEAT Predicted promoters ==<br />
[[Team:Heidelberg/HEARTBEAT_database| ''Main article: HEARTBEAT Database'']]<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" |SREBP regulated promoter, predicted, tested HB8 || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203125 Part:BBa_K203125 ]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" |SREBP regulated promoter, predicted, tested HB9 || Regulatory || [http://partsregistry.org/Part:BBa_K203126 Part:BBa_K203126]<br />
|-<br />
|}<br />
<br />
== Natural promoters ==<br />
<br />
[[Team:Heidelberg/Project_Natural_promoters|''Main article: Natural promoters'']]<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | LDL receptor promoter (sterol regulated) || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203105 Part:BBa_K203105]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | HMG-CoA synthase promoter (sterol regulated) || Regulatory || [http://partsregistry.org/Part:BBa_K203106 Part:BBa_K203106]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | c-Jun promoter (growth factor regulated) || Regulatory || [http://partsregistry.org/Part:BBa_K203107 Part:BBa_K203107]<br />
|-<br />
|}<br />
<br />
== Targeting Signals ==<br />
<br />
[[Team:Heidelberg/Project_SaO|''Main article: Outlook and Summary'']]<br />
<br />
Protein domains for targeting to sub-cellular compartments.<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Plasma membrane targeting (GPI anchor) || Protein domain || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203000 Part:BBa_K203000]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Plasma membrane targeting (myrpalm) || Protein domain || [http://partsregistry.org/Part:BBa_K203108 Part:BBa_K203108]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | ER membrane targeting (C-terminal) || Protein domain || [http://partsregistry.org/Part:BBa_K203101 Part:BBa_K203101]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | ER membrane targeting (N-terminal) || Protein domain || [http://partsregistry.org/Part:BBa_K203124 Part:BBa_K203124]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | mCherry-myrpalm-polyA || Composite || [http://partsregistry.org/Part:BBa_K203116 Part:BBa_K203116]<br />
|-<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | GFP-polyA || Composite || [http://partsregistry.org/Part:BBa_K203117 Part:BBa_K203117]<br />
|-<br />
|}<br />
<br />
|width="250px" style="padding: 0 20px 15px 15px; background-color:#d8d5d0"|<br />
<br />
|}</div>Ponyhttp://2009.igem.org/Team:Heidelberg/PartsTeam:Heidelberg/Parts2011-07-04T09:06:21Z<p>Pony: /* Parts */</p>
<hr />
<div>{{Template_HD_3}}<br />
<html><body id="parts"></body></html><br />
{|<br />
|-valign="top" border="0" style="margin-left: 2px;"<br />
|width="650px" style="padding: 0 15px 15px 20px; background-color:#ede8e2"|<br />
__NOTOC__<br />
= Parts =<br />
---<br />
== Measurement Kit ==<br />
[[Team:Heidelberg/Project_Measurement|''Main Article: Measurement'']]<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | pSMB_MEASURE: Promoter Measurement plasmid (mammalian) || Plasmid backbone || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" |pSMB_REFERENCE || Plasmid || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203099 Part:BBa_K203099]<br />
|-<br />
|}<br />
<br />
== Core promoters ==<br />
<br />
[[Team:Heidelberg/Project_Measurement#Different_core_promoters_result_in_different_expression_strength|''Main Article: Core promoters'']]<br />
<br />
The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by [[Project_Measurement#Different_core_promoters_result_in_different_expression_strength|core promoter swapping]].<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | JeT || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | JeT proximal-CMV core || Regulatory || [http://partsregistry.org/Part:BBa_K203113 Part:BBa_K203113]<br />
|-<br />
|}<br />
<br />
== Promoters created by RA-PCR ==<br />
<br />
[[Team:Heidelberg/Project_Synthetic_promoters|''Main article: Synthetic promoters'']]<br />
<br />
All contain the core promoter of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112] and were screened / measured in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100].<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Constitutive promoter; 0.4 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203110 Part:BBa_K203110]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Constitutive promoter; 1 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203118 Part:BBa_K203118]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Constitutive promoter; 2 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203111 Part:BBa_K203111]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Constitutive promoter; 2.5 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203109 Part:BBa_K203109]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | NF-&kappa;B regulated promoter || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203119 Part:BBa_K203119]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | pPAR&gamma; regulated promoter 1 || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203114 Part:BBa_K203114]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | pPAR&gamma; regulated promoter 2 || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203115 Part:BBa_K203115]<br />
|-<br />
|}<br />
<br />
== HEARTBEAT Predicted promoters ==<br />
[[Team:Heidelberg/HEARTBEAT_database| ''Main article: HEARTBEAT Database'']]<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" |SREBP regulated promoter, predicted, tested HB8 || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203125 Part:BBa_K203125 ]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" |SREBP regulated promoter, predicted, tested HB9 || Regulatory || [http://partsregistry.org/Part:BBa_K203126 Part:BBa_K203126]<br />
|-<br />
|}<br />
<br />
== Natural promoters ==<br />
<br />
[[Team:Heidelberg/Project_Natural_promoters|''Main article: Natural promoters'']]<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | LDL receptor promoter (sterol regulated) || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203105 Part:BBa_K203105]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | HMG-CoA synthase promoter (sterol regulated) || Regulatory || [http://partsregistry.org/Part:BBa_K203106 Part:BBa_K203106]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | c-Jun promoter (growth factor regulated) || Regulatory || [http://partsregistry.org/Part:BBa_K203107 Part:BBa_K203107]<br />
|-<br />
|}<br />
<br />
== Targeting Signals ==<br />
<br />
[[Team:Heidelberg/Project_SaO|''Main article: Outlook and Summary'']]<br />
<br />
Protein domains for targeting to sub-cellular compartments.<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Plasma membrane targeting (GPI anchor) || Protein domain || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203000 Part:BBa_K203000]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Plasma membrane targeting (myrpalm) || Protein domain || [http://partsregistry.org/Part:BBa_K203108 Part:BBa_K203108]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | ER membrane targeting (C-terminal) || Protein domain || [http://partsregistry.org/Part:BBa_K203101 Part:BBa_K203101]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | ER membrane targeting (N-terminal) || Protein domain || [http://partsregistry.org/Part:BBa_K203124 Part:BBa_K203124]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | mCherry-myrpalm-polyA || Composite || [http://partsregistry.org/Part:BBa_K203116 Part:BBa_K203116]<br />
|-<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | GFP-polyA || Composite || [http://partsregistry.org/Part:BBa_K203117 Part:BBa_K203117]<br />
|-<br />
|}<br />
<br />
|width="250px" style="padding: 0 20px 15px 15px; background-color:#d8d5d0"|<br />
<br />
|}</div>Ponyhttp://2009.igem.org/Team:Heidelberg/PartsTeam:Heidelberg/Parts2011-07-04T09:05:56Z<p>Pony: /* Parts */</p>
<hr />
<div>{{Template_HD_3}}<br />
<html><body id="parts"></body></html><br />
{|<br />
|-valign="top" border="0" style="margin-left: 2px;"<br />
|width="650px" style="padding: 0 15px 15px 20px; background-color:#ede8e2"|<br />
__NOTOC__<br />
= Parts =<br />
<br />
== Measurement Kit ==<br />
[[Team:Heidelberg/Project_Measurement|''Main Article: Measurement'']]<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | pSMB_MEASURE: Promoter Measurement plasmid (mammalian) || Plasmid backbone || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" |pSMB_REFERENCE || Plasmid || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203099 Part:BBa_K203099]<br />
|-<br />
|}<br />
<br />
== Core promoters ==<br />
<br />
[[Team:Heidelberg/Project_Measurement#Different_core_promoters_result_in_different_expression_strength|''Main Article: Core promoters'']]<br />
<br />
The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by [[Project_Measurement#Different_core_promoters_result_in_different_expression_strength|core promoter swapping]].<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | JeT || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | JeT proximal-CMV core || Regulatory || [http://partsregistry.org/Part:BBa_K203113 Part:BBa_K203113]<br />
|-<br />
|}<br />
<br />
== Promoters created by RA-PCR ==<br />
<br />
[[Team:Heidelberg/Project_Synthetic_promoters|''Main article: Synthetic promoters'']]<br />
<br />
All contain the core promoter of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112] and were screened / measured in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100].<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Constitutive promoter; 0.4 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203110 Part:BBa_K203110]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Constitutive promoter; 1 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203118 Part:BBa_K203118]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Constitutive promoter; 2 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203111 Part:BBa_K203111]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Constitutive promoter; 2.5 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203109 Part:BBa_K203109]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | NF-&kappa;B regulated promoter || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203119 Part:BBa_K203119]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | pPAR&gamma; regulated promoter 1 || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203114 Part:BBa_K203114]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | pPAR&gamma; regulated promoter 2 || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203115 Part:BBa_K203115]<br />
|-<br />
|}<br />
<br />
== HEARTBEAT Predicted promoters ==<br />
[[Team:Heidelberg/HEARTBEAT_database| ''Main article: HEARTBEAT Database'']]<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" |SREBP regulated promoter, predicted, tested HB8 || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203125 Part:BBa_K203125 ]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" |SREBP regulated promoter, predicted, tested HB9 || Regulatory || [http://partsregistry.org/Part:BBa_K203126 Part:BBa_K203126]<br />
|-<br />
|}<br />
<br />
== Natural promoters ==<br />
<br />
[[Team:Heidelberg/Project_Natural_promoters|''Main article: Natural promoters'']]<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | LDL receptor promoter (sterol regulated) || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203105 Part:BBa_K203105]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | HMG-CoA synthase promoter (sterol regulated) || Regulatory || [http://partsregistry.org/Part:BBa_K203106 Part:BBa_K203106]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | c-Jun promoter (growth factor regulated) || Regulatory || [http://partsregistry.org/Part:BBa_K203107 Part:BBa_K203107]<br />
|-<br />
|}<br />
<br />
== Targeting Signals ==<br />
<br />
[[Team:Heidelberg/Project_SaO|''Main article: Outlook and Summary'']]<br />
<br />
Protein domains for targeting to sub-cellular compartments.<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Plasma membrane targeting (GPI anchor) || Protein domain || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203000 Part:BBa_K203000]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Plasma membrane targeting (myrpalm) || Protein domain || [http://partsregistry.org/Part:BBa_K203108 Part:BBa_K203108]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | ER membrane targeting (C-terminal) || Protein domain || [http://partsregistry.org/Part:BBa_K203101 Part:BBa_K203101]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | ER membrane targeting (N-terminal) || Protein domain || [http://partsregistry.org/Part:BBa_K203124 Part:BBa_K203124]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | mCherry-myrpalm-polyA || Composite || [http://partsregistry.org/Part:BBa_K203116 Part:BBa_K203116]<br />
|-<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | GFP-polyA || Composite || [http://partsregistry.org/Part:BBa_K203117 Part:BBa_K203117]<br />
|-<br />
|}<br />
<br />
|width="250px" style="padding: 0 20px 15px 15px; background-color:#d8d5d0"|<br />
<br />
|}</div>Ponyhttp://2009.igem.org/Team:Heidelberg/PartsTeam:Heidelberg/Parts2011-07-04T09:05:27Z<p>Pony: /* Measurement Kit */</p>
<hr />
<div>{{Template_HD_3}}<br />
<html><body id="parts"></body></html><br />
{|<br />
|-valign="top" border="0" style="margin-left: 2px;"<br />
|width="650px" style="padding: 0 15px 15px 20px; background-color:#ede8e2"|<br />
__NOTOC__<br />
= Parts =<br />
<br />
Measurement Kit<br />
[[Team:Heidelberg/Project_Measurement|''Main Article: Measurement'']]<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | pSMB_MEASURE: Promoter Measurement plasmid (mammalian) || Plasmid backbone || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" |pSMB_REFERENCE || Plasmid || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203099 Part:BBa_K203099]<br />
|-<br />
|}<br />
<br />
== Core promoters ==<br />
<br />
[[Team:Heidelberg/Project_Measurement#Different_core_promoters_result_in_different_expression_strength|''Main Article: Core promoters'']]<br />
<br />
The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by [[Project_Measurement#Different_core_promoters_result_in_different_expression_strength|core promoter swapping]].<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | JeT || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | JeT proximal-CMV core || Regulatory || [http://partsregistry.org/Part:BBa_K203113 Part:BBa_K203113]<br />
|-<br />
|}<br />
<br />
== Promoters created by RA-PCR ==<br />
<br />
[[Team:Heidelberg/Project_Synthetic_promoters|''Main article: Synthetic promoters'']]<br />
<br />
All contain the core promoter of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112] and were screened / measured in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100].<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Constitutive promoter; 0.4 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203110 Part:BBa_K203110]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Constitutive promoter; 1 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203118 Part:BBa_K203118]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Constitutive promoter; 2 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203111 Part:BBa_K203111]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Constitutive promoter; 2.5 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203109 Part:BBa_K203109]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | NF-&kappa;B regulated promoter || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203119 Part:BBa_K203119]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | pPAR&gamma; regulated promoter 1 || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203114 Part:BBa_K203114]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | pPAR&gamma; regulated promoter 2 || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203115 Part:BBa_K203115]<br />
|-<br />
|}<br />
<br />
== HEARTBEAT Predicted promoters ==<br />
[[Team:Heidelberg/HEARTBEAT_database| ''Main article: HEARTBEAT Database'']]<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" |SREBP regulated promoter, predicted, tested HB8 || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203125 Part:BBa_K203125 ]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" |SREBP regulated promoter, predicted, tested HB9 || Regulatory || [http://partsregistry.org/Part:BBa_K203126 Part:BBa_K203126]<br />
|-<br />
|}<br />
<br />
== Natural promoters ==<br />
<br />
[[Team:Heidelberg/Project_Natural_promoters|''Main article: Natural promoters'']]<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | LDL receptor promoter (sterol regulated) || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203105 Part:BBa_K203105]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | HMG-CoA synthase promoter (sterol regulated) || Regulatory || [http://partsregistry.org/Part:BBa_K203106 Part:BBa_K203106]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | c-Jun promoter (growth factor regulated) || Regulatory || [http://partsregistry.org/Part:BBa_K203107 Part:BBa_K203107]<br />
|-<br />
|}<br />
<br />
== Targeting Signals ==<br />
<br />
[[Team:Heidelberg/Project_SaO|''Main article: Outlook and Summary'']]<br />
<br />
Protein domains for targeting to sub-cellular compartments.<br />
<br />
{| class="wikitable centered" style="margin: 1em auto 1em auto"<br />
|- style="background-color:#99cccc;" <br />
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Plasma membrane targeting (GPI anchor) || Protein domain || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203000 Part:BBa_K203000]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | Plasma membrane targeting (myrpalm) || Protein domain || [http://partsregistry.org/Part:BBa_K203108 Part:BBa_K203108]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | ER membrane targeting (C-terminal) || Protein domain || [http://partsregistry.org/Part:BBa_K203101 Part:BBa_K203101]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | ER membrane targeting (N-terminal) || Protein domain || [http://partsregistry.org/Part:BBa_K203124 Part:BBa_K203124]<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | mCherry-myrpalm-polyA || Composite || [http://partsregistry.org/Part:BBa_K203116 Part:BBa_K203116]<br />
|-<br />
|- style="background-color:#9BDDFF;" <br />
| style="font-weight:bold;" | GFP-polyA || Composite || [http://partsregistry.org/Part:BBa_K203117 Part:BBa_K203117]<br />
|-<br />
|}<br />
<br />
|width="250px" style="padding: 0 20px 15px 15px; background-color:#d8d5d0"|<br />
<br />
|}</div>Pony