http://2009.igem.org/wiki/index.php?title=Special:Contributions/Wiltshir&feed=atom&limit=50&target=Wiltshir&year=&month=
2009.igem.org - User contributions [en]
2024-03-28T15:38:17Z
From 2009.igem.org
MediaWiki 1.16.5
http://2009.igem.org/User:Wiltshir
User:Wiltshir
2010-10-19T01:25:38Z
<p>Wiltshir: /* Zach Wiltshire */</p>
<hr />
<div><br><br />
<br />
===Zach Wiltshire===<br />
<br />
zach [dot] wiltshire [at] ualberta [dot] ca<br />
<br />
[[https://2009.igem.org/Team:Alberta<nowiki>2009 iGEM Project </nowiki>]] - Project BioBytes<br />
<br><br />
[[https://2008.igem.org/Team:Alberta_NINT<nowiki>2008 iGEM Project </nowiki>]] - Project Logi-coli<br />
<br />
<br><br></div>
Wiltshir
http://2009.igem.org/Team:Alberta/TemplateSc
Team:Alberta/TemplateSc
2009-10-22T01:50:54Z
<p>Wiltshir: </p>
<hr />
<div><html><br />
<title>University of Alberta - BioBytes</title><br />
<br />
<link rel="stylesheet" type="text/css" href="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/main.css&action=raw&ctype=text/css" /><br />
<link rel="stylesheet" type="text/css" href="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/dd.css&action=raw&ctype=text/css" /><br />
<!--[if IE]><br />
<style type="text/css"><br />
#utilNav ul li {list-style-image: none;}<br />
#suppNav ul li {list-style-image: none;}<br />
#mainNav ul li {list-style-image: none;}<br />
</style><br />
<![endif]--><br />
<script type="text/javascript" src="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/java.js&action=raw&ctype=text/javascript"></script><br />
<script type="text/javascript"><br />
function ddnav() {<br />
$('#ddNav li').hover(<br />
function () {<br />
$(this).find('div:first').css('display','block');},<br />
function () {<br />
$(this).find('div:first').css('display','none');}<br />
);<br />
}<br />
function ddnavsub() {<br />
$('#ddNav span > a').toggle(<br />
function () {<br />
$(this).removeClass("expand").addClass("collapse");<br />
$(this).parent().find('div:first').slideDown('fast');<br />
$(this).hover(<br />
function (){$(this).css('background-color','#e5e5e0')},<br />
function (){$(this).css('background-color','#e5e5e0')});},<br />
function () {<br />
$(this).removeClass("collapse").addClass("expand");<br />
$(this).parent().find('div:first').css('display','none');<br />
$(this).hover(<br />
function (){$(this).css('background-color','#F5F5F0')},<br />
function (){$(this).css('background-color','#FFFFFF')});}<br />
).addClass('expand');<br />
}<br />
<br />
function ddmozilla() {<br />
ddtoggle();<br />
}<br />
<br />
$(function () {<br />
ddnav();<br />
ddnavsub();<br />
<br />
if($.browser.mozilla) {ddmozilla();}<br />
});<br />
</script><br />
<center><br />
<div id="header"><br />
<img src="https://static.igem.org/mediawiki/2009/archive/c/c7/20091022014726!UofA_biobytes_HeaderBanner6.png" width="965" height="235"><br />
<br />
<!-- logo --><br />
<div id="logo"><br />
</div><br />
<!-- END logo --><br />
<br />
<!-- main navigation --><br />
<div id="mainNav"><br />
<ul id="ddNav"><br />
<br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta">&nbsp; Home &nbsp; </a><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/Team">&nbsp; Team &nbsp; </a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Team">Undergraduates</a><br />
<a href="https://2009.igem.org/Team:Alberta/Team#Team_Ad">Team Advisors</a><br />
<a href="https://2009.igem.org/Team:Alberta/Team#Fac_Con">Faculty Consultants</a><br />
</div><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta">&nbsp;Project&nbsp;</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Project/assemblyoverview">DNA Assembly</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/AutomationOverview">Automation</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Bioinformatics">Organism Design</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Chromosome_Assembly">Chromosome Assembly</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Outreach">Human Practices</a><br />
</div><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/MedalRequirements">&nbsp;Achievements&nbsp;</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/MedalRequirements">Medals & Prizes</a><br />
<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Alberta" target="_blank">Parts Registry</a><br />
<br />
</div><br />
</li><br />
<li><br />
<a>&nbsp; &nbsp; Lab &nbsp; &nbsp; </a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Calendar">Lab Notebook</a><br />
<a href="https://2009.igem.org/Team:Alberta/Blackboard">Lab Blackboard</a><br />
<a href="https://2009.igem.org/Team:Alberta/Protocols">Protocols</a><br />
<br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/References/Keyword">&nbsp;References&nbsp;</a><br />
</li><br />
<br />
</ul><br />
<br />
</div><br />
<br />
<!-- END main navigation --><br />
<br />
</div><br />
</center><br />
</html><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br />
{{:Team:Alberta/LeftBar}}<br />
<br />
<html><br />
<style type="text/css"><br />
body {background-color:#f8f8f8;}<br />
</style><br />
</html></div>
Wiltshir
http://2009.igem.org/Team:Alberta/Template3
Team:Alberta/Template3
2009-10-22T01:49:31Z
<p>Wiltshir: </p>
<hr />
<div><html><br />
<title>University of Alberta - BioBytes</title><br />
<br />
<link rel="stylesheet" type="text/css" href="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/main.css&action=raw&ctype=text/css" /><br />
<link rel="stylesheet" type="text/css" href="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/dd.css&action=raw&ctype=text/css" /><br />
<!--[if IE]><br />
<style type="text/css"><br />
#utilNav ul li {list-style-image: none;}<br />
#suppNav ul li {list-style-image: none;}<br />
#mainNav ul li {list-style-image: none;}<br />
</style><br />
<![endif]--><br />
<script type="text/javascript" src="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/java.js&action=raw&ctype=text/javascript"></script><br />
<script type="text/javascript"><br />
function ddnav() {<br />
$('#ddNav li').hover(<br />
function () {<br />
$(this).find('div:first').css('display','block');},<br />
function () {<br />
$(this).find('div:first').css('display','none');}<br />
);<br />
}<br />
function ddnavsub() {<br />
$('#ddNav span > a').toggle(<br />
function () {<br />
$(this).removeClass("expand").addClass("collapse");<br />
$(this).parent().find('div:first').slideDown('fast');<br />
$(this).hover(<br />
function (){$(this).css('background-color','#e5e5e0')},<br />
function (){$(this).css('background-color','#e5e5e0')});},<br />
function () {<br />
$(this).removeClass("collapse").addClass("expand");<br />
$(this).parent().find('div:first').css('display','none');<br />
$(this).hover(<br />
function (){$(this).css('background-color','#F5F5F0')},<br />
function (){$(this).css('background-color','#FFFFFF')});}<br />
).addClass('expand');<br />
}<br />
<br />
function ddmozilla() {<br />
ddtoggle();<br />
}<br />
<br />
$(function () {<br />
ddnav();<br />
ddnavsub();<br />
<br />
if($.browser.mozilla) {ddmozilla();}<br />
});<br />
</script><br />
<center><br />
<div id="header"><br />
<img src="https://static.igem.org/mediawiki/2009/archive/c/c7/20091022014726!UofA_biobytes_HeaderBanner6.png" width="965" height="235"><br />
<br />
<!-- logo --><br />
<div id="logo"><br />
</div><br />
<!-- END logo --><br />
<br />
<!-- main navigation --><br />
<div id="mainNav"><br />
<ul id="ddNav"><br />
<br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta">&nbsp; Home &nbsp; </a><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/Team">&nbsp; Team &nbsp; </a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Team">Undergraduates</a><br />
<a href="https://2009.igem.org/Team:Alberta/Team#Team_Ad">Team Advisors</a><br />
<a href="https://2009.igem.org/Team:Alberta/Team#Fac_Con">Faculty Consultants</a><br />
</div><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta">&nbsp;Project&nbsp;</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Project/assemblyoverview">DNA Assembly</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/AutomationOverview">Automation</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Bioinformatics">Organism Design</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Chromosome_Assembly">Chromosome Assembly</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Outreach">Human Practices</a><br />
</div><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/MedalRequirements">&nbsp;Achievements&nbsp;</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/MedalRequirements">Medals & Prizes</a><br />
<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Alberta" target="_blank">Parts Registry</a><br />
<br />
</div><br />
</li><br />
<li><br />
<a>&nbsp; &nbsp; Lab &nbsp; &nbsp; </a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Calendar">Lab Notebook</a><br />
<a href="https://2009.igem.org/Team:Alberta/Blackboard">Lab Blackboard</a><br />
<a href="https://2009.igem.org/Team:Alberta/Protocols">Protocols</a><br />
<br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/References/Keyword">&nbsp;References&nbsp;</a><br />
</li><br />
<br />
</ul><br />
<br />
</div><br />
<br />
<!-- END main navigation --><br />
<br />
</div><br />
</center><br />
</html><br />
<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br />
{{:Team:Alberta/LeftBar}}<br />
{{:Team:Alberta/RightBar}}<br />
<html><br />
<style type="text/css"><br />
body {background-color:#f8f8f8;}<br />
</style><br />
</html></div>
Wiltshir
http://2009.igem.org/Team:Alberta/Template3
Team:Alberta/Template3
2009-10-22T01:48:32Z
<p>Wiltshir: </p>
<hr />
<div><html><br />
<title>University of Alberta - BioBytes</title><br />
<br />
<link rel="stylesheet" type="text/css" href="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/main.css&action=raw&ctype=text/css" /><br />
<link rel="stylesheet" type="text/css" href="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/dd.css&action=raw&ctype=text/css" /><br />
<!--[if IE]><br />
<style type="text/css"><br />
#utilNav ul li {list-style-image: none;}<br />
#suppNav ul li {list-style-image: none;}<br />
#mainNav ul li {list-style-image: none;}<br />
</style><br />
<![endif]--><br />
<script type="text/javascript" src="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/java.js&action=raw&ctype=text/javascript"></script><br />
<script type="text/javascript"><br />
function ddnav() {<br />
$('#ddNav li').hover(<br />
function () {<br />
$(this).find('div:first').css('display','block');},<br />
function () {<br />
$(this).find('div:first').css('display','none');}<br />
);<br />
}<br />
function ddnavsub() {<br />
$('#ddNav span > a').toggle(<br />
function () {<br />
$(this).removeClass("expand").addClass("collapse");<br />
$(this).parent().find('div:first').slideDown('fast');<br />
$(this).hover(<br />
function (){$(this).css('background-color','#e5e5e0')},<br />
function (){$(this).css('background-color','#e5e5e0')});},<br />
function () {<br />
$(this).removeClass("collapse").addClass("expand");<br />
$(this).parent().find('div:first').css('display','none');<br />
$(this).hover(<br />
function (){$(this).css('background-color','#F5F5F0')},<br />
function (){$(this).css('background-color','#FFFFFF')});}<br />
).addClass('expand');<br />
}<br />
<br />
function ddmozilla() {<br />
ddtoggle();<br />
}<br />
<br />
$(function () {<br />
ddnav();<br />
ddnavsub();<br />
<br />
if($.browser.mozilla) {ddmozilla();}<br />
});<br />
</script><br />
<center><br />
<div id="header"><br />
<img src="https://static.igem.org/mediawiki/2009/c/c7/UofA_biobytes_HeaderBanner6.png" width="965" height="235"><br />
<br />
<!-- logo --><br />
<div id="logo"><br />
</div><br />
<!-- END logo --><br />
<br />
<!-- main navigation --><br />
<div id="mainNav"><br />
<ul id="ddNav"><br />
<br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta">&nbsp; Home &nbsp; </a><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/Team">&nbsp; Team &nbsp; </a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Team">Undergraduates</a><br />
<a href="https://2009.igem.org/Team:Alberta/Team#Team_Ad">Team Advisors</a><br />
<a href="https://2009.igem.org/Team:Alberta/Team#Fac_Con">Faculty Consultants</a><br />
</div><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta">&nbsp;Project&nbsp;</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Project/assemblyoverview">DNA Assembly</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/AutomationOverview">Automation</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Bioinformatics">Organism Design</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Chromosome_Assembly">Chromosome Assembly</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Outreach">Human Practices</a><br />
</div><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/MedalRequirements">&nbsp;Achievements&nbsp;</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/MedalRequirements">Medals & Prizes</a><br />
<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Alberta" target="_blank">Parts Registry</a><br />
<br />
</div><br />
</li><br />
<li><br />
<a>&nbsp; &nbsp; Lab &nbsp; &nbsp; </a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Calendar">Lab Notebook</a><br />
<a href="https://2009.igem.org/Team:Alberta/Blackboard">Lab Blackboard</a><br />
<a href="https://2009.igem.org/Team:Alberta/Protocols">Protocols</a><br />
<br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/References/Keyword">&nbsp;References&nbsp;</a><br />
</li><br />
<br />
</ul><br />
<br />
</div><br />
<br />
<!-- END main navigation --><br />
<br />
</div><br />
</center><br />
</html><br />
<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br />
{{:Team:Alberta/LeftBar}}<br />
{{:Team:Alberta/RightBar}}<br />
<html><br />
<style type="text/css"><br />
body {background-color:#f8f8f8;}<br />
</style><br />
</html></div>
Wiltshir
http://2009.igem.org/File:UofA_biobytes_HeaderBanner6.png
File:UofA biobytes HeaderBanner6.png
2009-10-22T01:47:26Z
<p>Wiltshir: uploaded a new version of "Image:UofA biobytes HeaderBanner6.png"</p>
<hr />
<div></div>
Wiltshir
http://2009.igem.org/File:UofA_biobytes_HeaderBanner6.png
File:UofA biobytes HeaderBanner6.png
2009-10-22T01:28:53Z
<p>Wiltshir: </p>
<hr />
<div></div>
Wiltshir
http://2009.igem.org/Team:Alberta/LeftBar
Team:Alberta/LeftBar
2009-10-21T23:44:30Z
<p>Wiltshir: </p>
<hr />
<div><html><br />
<head><br />
<style type="text/css"><br />
<!--<br />
ul#menu {<br />
width: 158px;<br />
list-style: none;<br />
border-top: solid 1px #b9a894;<br />
margin: 20px13px7px;<br />
padding: 0;<br />
position: relative;<br />
float: left;<br />
}<br />
<br />
ul#menu ol {<br />
display: none;<br />
text-align: center;<br />
list-style-type: none;<br />
margin: 0px 0;<br />
padding: 0;<br />
}<br />
<br />
ul#menu li, <br />
ul#menu a {<br />
font-family: "Trebuchet MS", Arial, Sans-serif;<br />
font-size: 13px;<br />
color: #000000;<br />
margin: 0;<br />
}<br />
<br />
ul#menu li {<br />
border-bottom: solid 1px #b9a894;<br />
line-height: 15px;<br />
}<br />
<br />
ul#menu ol li {<br />
border-bottom: none;<br />
}<br />
<br />
ul#menu ol li:before {<br />
content: "";<br />
}<br />
<br />
ul#menu a {<br />
display: block;<br />
width: 150px;<br />
height: 19px;<br />
border: 1px solid #bbb;<br />
margin-top: 0px;<br />
background: url(https://static.igem.org/mediawiki/2009/0/03/UofA_MainLink.gif) no-repeat left top;<br />
padding: 2px 4px;<br />
text-decoration: none;<br />
color: #000<br />
outline: none;<br />
}<br />
<br />
ul#menu a:hover {<br />
display: block;<br />
width: 150px;<br />
height: 19px;<br />
border: 1px solid #bbb;<br />
margin-top: 1px;<br />
background: url(https://static.igem.org/mediawiki/2009/a/a2/UofA_HoverLink.gif) no-repeat left top;<br />
padding: 2px 4px;<br />
color: #0000ff;<br />
}<br />
<br />
ul#menu a.active {<br />
display: block;<br />
margin: 0;<br />
padding: 2px 4px;<br />
width: 150px;<br />
text-decoration: none;<br />
font-weight: bold;<br />
background: url(https://static.igem.org/mediawiki/2009/1/10/UofA_Main.gif) repeat-x bottom;<br />
}<br />
--><br />
</style><br />
<br />
<script type="text/javascript"><br />
<!--<br />
/* This script and many more are available free online at<br />
The JavaScript Source :: http://javascript.internet.com<br />
Created by: Travis Beckham :: http://www.squidfingers.com | http://www.podlob.com */<br />
<br />
/* Coded by Travis Beckham<br />
http://www.squidfingers.com | http://www.podlob.com<br />
version date: 06/02/03<br />
If want to use this code, feel free to do so, but please leave this message intact. */<br />
<br />
// Node Functions<br />
<br />
if(!window.Node){<br />
var Node = {ELEMENT_NODE : 1, TEXT_NODE : 3};<br />
}<br />
<br />
function checkNode(node, filter){<br />
return (filter == null || node.nodeType == Node[filter] || node.nodeName.toUpperCase() == filter.toUpperCase());<br />
}<br />
<br />
function getChildren(node, filter){<br />
var result = new Array();<br />
var children = node.childNodes;<br />
for(var i = 0; i < children.length; i++){<br />
if(checkNode(children[i], filter)) result[result.length] = children[i];<br />
}<br />
return result;<br />
}<br />
<br />
function getChildrenByElement(node){<br />
return getChildren(node, "ELEMENT_NODE");<br />
}<br />
<br />
function getFirstChild(node, filter){<br />
var child;<br />
var children = node.childNodes;<br />
for(var i = 0; i < children.length; i++){<br />
child = children[i];<br />
if(checkNode(child, filter)) return child;<br />
}<br />
return null;<br />
}<br />
<br />
function getFirstChildByText(node){<br />
return getFirstChild(node, "TEXT_NODE");<br />
}<br />
<br />
function getNextSibling(node, filter){<br />
for(var sibling = node.nextSibling; sibling != null; sibling = sibling.nextSibling){<br />
if(checkNode(sibling, filter)) return sibling;<br />
}<br />
return null;<br />
}<br />
function getNextSiblingByElement(node){<br />
return getNextSibling(node, "ELEMENT_NODE");<br />
}<br />
<br />
// Menu Functions & Properties<br />
<br />
var activeMenu;<br />
var lastClicked;<br />
<br />
function showMenu() {<br />
if(activeMenu){<br />
activeMenu.className = "";<br />
getNextSiblingByElement(activeMenu).style.display = "none";<br />
}<br />
if(this == activeMenu)<br />
{<br />
activeMenu = null;<br />
lastClicked = null;<br />
} else {<br />
this.className = "active";<br />
getNextSiblingByElement(this).style.display = "block";<br />
activeMenu = this;<br />
lastClicked = this.textContent;<br />
window.name = lastClicked;<br />
} <br />
<br />
return false;<br />
<br />
<br />
}<br />
<br />
function initMenu(){<br />
var menus, menu, text, a, i;<br />
menus = getChildrenByElement(document.getElementById("menu"));<br />
<br />
for(i = 0; i < menus.length; i++){<br />
menu = menus[i];<br />
text = getFirstChildByText(menu);<br />
a = document.createElement("a");<br />
menu.replaceChild(a, text);<br />
a.appendChild(text);<br />
a.href = "#";<br />
a.onclick = showMenu;<br />
a.onfocus = function(){this.blur()};<br />
if (a.textContent == window.name)<br />
{<br />
a.className = "active";<br />
getNextSiblingByElement(a).style.display = "block";<br />
activeMenu = a;<br />
}<br />
<br />
}<br />
<br />
<br />
}<br />
<br />
if(document.createElement) window.onload = initMenu;<br />
//--><br />
</script><br />
<br />
<br />
</head><br />
<body><br />
<br />
<table align=left cellspacing=0 cellpadding=0><br />
<tr><td><BR><img src="https://static.igem.org/mediawiki/2009/e/ed/UofA09_MainPrjt.png" hspace=19></td></tr><br />
<tr><td><br />
<ul id="menu"><br />
<li>DNA Assembly<br />
<ol><br />
<lie><a href="https://2009.igem.org/Team:Alberta/Project/assemblyoverview">Overview</a><br />
<li><a href="https://2009.igem.org/Team:Alberta/ByteCreation">Byte Creation</a></li><br />
<li><a href="https://2009.igem.org/Team:Alberta/DNAanchor">Anchor/Terminator</a></li><br />
<li><a href="https://2009.igem.org/Team:Alberta/Optimization">Optimization</a></li><br />
<li><a href="https://2009.igem.org/Team:Alberta/Project/linearconstruct">Linear Constructs</a></li><br />
<li><a href="https://2009.igem.org/Team:Alberta/plasmidconstruct">Plasmid Constructs</a></li><br />
</ol><br />
</li><br />
<li>Automation<br />
<ol><br />
<li><a href="https://2009.igem.org/Team:Alberta/Project/AutomationOverview">Overview</a></li><br />
<li><a href="https://2009.igem.org/Team:Alberta/Project/Microfluidics">Microfluidics</a></li><br />
<li><a href="https://2009.igem.org/Team:Alberta/Project/Automation">DIY Automation</a></li><br />
</ol><br />
</li><br />
<li>Organism Design<br />
<ol><br />
<li><a href="https://2009.igem.org/Team:Alberta/Project/Bioinformatics">Overview</a><br />
<li><a href="https://2009.igem.org/Team:Alberta/Project/Gene_Selection">Gene Selection</a></li><br />
<li><a href="https://2009.igem.org/Team:Alberta/Project/Modeling">Modeling</a></li><br />
<li><a href="https://2009.igem.org/Team:Alberta/Project/ModelValid">Model Validation</a></li><br />
<li><a href="https://2009.igem.org/Team:Alberta/Project/Promoters_&_Terminators">Promoter/Terminator</a></li><br />
<li><a href="https://2009.igem.org/Team:Alberta/Project/Primer_Design">Primer Design</a></li><br />
</ol><br />
</li><br />
<li>Chromosome Assembly<br />
<ol><br />
<li><a href="https://2009.igem.org/Team:Alberta/Project/Chromosome Assembly">Overview</a></li><br />
<li><a href="https://2009.igem.org/Team:Alberta/Project/Recombineering">Recombineering</a></li><br />
</ol><br />
<li>Human Practices<br />
<ol><br />
<li><a href="https://2009.igem.org/Team:Alberta/Project/Outreach">Overview</a></li><br />
<li><a href="https://2009.igem.org/Team:Alberta/Project/Presentations">Presentations</a></li><br />
<li><a href="https://2009.igem.org/Team:Alberta/Project/Surveys">Feedback</a></li><br />
<li><a href="https://2009.igem.org/Team:Alberta/Project/UofADebate">U of A Debate</a></li><br />
<li><a href="https://2009.igem.org/Team:Alberta/Project/Debate_Outreach"> HS Debate Outreach</a></li><br />
<li><a href="https://2009.igem.org/Team:Alberta/Project/Safety">Safety</a></li><br />
</ol><br />
</li><br />
</ul><br />
<BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><br />
</td></tr></table><br />
<br />
<!--<br />
<div id="menu"><br />
<ul><br />
<li>[[Team:Alberta | Home]]</li><br />
</ul><br />
<br />
<ul><br />
<li class="menu_header">Project</li></ul><br />
<hr><br />
<hr><br />
<br />
<ul id="menu>><br />
<br />
<li>[[Team:Alberta/Project/Bioinformatics | '''Bioinformatics''']]<br />
<ol><li>[[Team:Alberta/Project/Primer_Design | Primer Design]]</li><br />
<li>[[Team:Alberta/Project/Promoters_&_Terminators | Promoters/Terminators]]</li><br />
<li>[[Team:Alberta/Project/Modeling | Modeling]]</li><br />
<li>[[Team:Alberta/Project/Gene_Selection | Gene Selection]]</li><br />
</ol><br />
</li><br />
</ul><br />
<br />
<hr><br />
<hr><br />
<li>[[Team:Alberta/Project/Automation | '''Automation''']]</li><br />
<li>[[Team:Alberta/Project/Microfluidics | Microfluidics]]</li><br />
<hr><br />
<hr><br />
<li>[[Team:Alberta/Project/Assembly_Process | '''Assembly Process''']]</li><br />
<hr><br />
<hr><br />
<li>[[Team:Alberta/Project/Recombineering | '''Recombineering''']]</li><br />
<hr><br />
<hr><br />
<li>[[Team:Alberta/Project/Cell_Reboot | '''Cell Reboot''']]</li><br />
<hr><br />
<hr><br />
<li>[[Team:Alberta/Project/Parts | '''Parts''']]</li><br />
<hr><br />
<hr><br />
<li>[[Team:Alberta/Project/Outreach | '''Ethics Debate''']]</li><br />
<hr><br />
<hr><br />
<li>[[Team:Alberta/Project/Parts | '''Outreach''']]</li><br />
</ul><br />
<br />
</div><br />
--><br />
</body><br />
<br />
</html></div>
Wiltshir
http://2009.igem.org/Team:Alberta/Template3
Team:Alberta/Template3
2009-10-21T23:34:55Z
<p>Wiltshir: </p>
<hr />
<div><html><br />
<title>University of Alberta - BioBytes</title><br />
<br />
<link rel="stylesheet" type="text/css" href="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/main.css&action=raw&ctype=text/css" /><br />
<link rel="stylesheet" type="text/css" href="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/dd.css&action=raw&ctype=text/css" /><br />
<!--[if IE]><br />
<style type="text/css"><br />
#utilNav ul li {list-style-image: none;}<br />
#suppNav ul li {list-style-image: none;}<br />
#mainNav ul li {list-style-image: none;}<br />
</style><br />
<![endif]--><br />
<script type="text/javascript" src="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/java.js&action=raw&ctype=text/javascript"></script><br />
<script type="text/javascript"><br />
function ddnav() {<br />
$('#ddNav li').hover(<br />
function () {<br />
$(this).find('div:first').css('display','block');},<br />
function () {<br />
$(this).find('div:first').css('display','none');}<br />
);<br />
}<br />
function ddnavsub() {<br />
$('#ddNav span > a').toggle(<br />
function () {<br />
$(this).removeClass("expand").addClass("collapse");<br />
$(this).parent().find('div:first').slideDown('fast');<br />
$(this).hover(<br />
function (){$(this).css('background-color','#e5e5e0')},<br />
function (){$(this).css('background-color','#e5e5e0')});},<br />
function () {<br />
$(this).removeClass("collapse").addClass("expand");<br />
$(this).parent().find('div:first').css('display','none');<br />
$(this).hover(<br />
function (){$(this).css('background-color','#F5F5F0')},<br />
function (){$(this).css('background-color','#FFFFFF')});}<br />
).addClass('expand');<br />
}<br />
<br />
function ddmozilla() {<br />
ddtoggle();<br />
}<br />
<br />
$(function () {<br />
ddnav();<br />
ddnavsub();<br />
<br />
if($.browser.mozilla) {ddmozilla();}<br />
});<br />
</script><br />
<center><br />
<div id="header"><br />
<img src="https://static.igem.org/mediawiki/2009/0/04/UofA_biobytes_HeaderBanner5.png" width="965" height="235"><br />
<br />
<!-- logo --><br />
<div id="logo"><br />
</div><br />
<!-- END logo --><br />
<br />
<!-- main navigation --><br />
<div id="mainNav"><br />
<ul id="ddNav"><br />
<br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta">&nbsp; Home &nbsp; </a><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/Team">&nbsp; Team &nbsp; </a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Team">Undergraduates</a><br />
<a href="https://2009.igem.org/Team:Alberta/Team#Team_Ad">Team Advisors</a><br />
<a href="https://2009.igem.org/Team:Alberta/Team#Fac_Con">Faculty Consultants</a><br />
</div><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta">&nbsp;Project&nbsp;</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Project/assemblyoverview">DNA Assembly</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/AutomationOverview">Automation</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Bioinformatics">Organism Design</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Chromosome_Assembly">Chromosome Assembly</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Outreach">Human Practices</a><br />
</div><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/MedalRequirements">&nbsp;Achievements&nbsp;</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/MedalRequirements">Medals & Prizes</a><br />
<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Alberta" target="_blank">Parts Registry</a><br />
<br />
</div><br />
</li><br />
<li><br />
<a>&nbsp; &nbsp; Lab &nbsp; &nbsp; </a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Calendar">Lab Notebook</a><br />
<a href="https://2009.igem.org/Team:Alberta/Blackboard">Lab Blackboard</a><br />
<a href="https://2009.igem.org/Team:Alberta/Protocols">Protocols</a><br />
<br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/References/Keyword">&nbsp;References&nbsp;</a><br />
</li><br />
<br />
</ul><br />
<br />
</div><br />
<br />
<!-- END main navigation --><br />
<br />
</div><br />
</center><br />
</html><br />
<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br />
{{:Team:Alberta/LeftBar}}<br />
{{:Team:Alberta/RightBar}}<br />
<html><br />
<style type="text/css"><br />
body {background-color:#f8f8f8;}<br />
</style><br />
</html></div>
Wiltshir
http://2009.igem.org/Team:Alberta/TemplateSc
Team:Alberta/TemplateSc
2009-10-21T23:33:57Z
<p>Wiltshir: </p>
<hr />
<div><html><br />
<title>University of Alberta - BioBytes</title><br />
<br />
<link rel="stylesheet" type="text/css" href="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/main.css&action=raw&ctype=text/css" /><br />
<link rel="stylesheet" type="text/css" href="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/dd.css&action=raw&ctype=text/css" /><br />
<!--[if IE]><br />
<style type="text/css"><br />
#utilNav ul li {list-style-image: none;}<br />
#suppNav ul li {list-style-image: none;}<br />
#mainNav ul li {list-style-image: none;}<br />
</style><br />
<![endif]--><br />
<script type="text/javascript" src="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/java.js&action=raw&ctype=text/javascript"></script><br />
<script type="text/javascript"><br />
function ddnav() {<br />
$('#ddNav li').hover(<br />
function () {<br />
$(this).find('div:first').css('display','block');},<br />
function () {<br />
$(this).find('div:first').css('display','none');}<br />
);<br />
}<br />
function ddnavsub() {<br />
$('#ddNav span > a').toggle(<br />
function () {<br />
$(this).removeClass("expand").addClass("collapse");<br />
$(this).parent().find('div:first').slideDown('fast');<br />
$(this).hover(<br />
function (){$(this).css('background-color','#e5e5e0')},<br />
function (){$(this).css('background-color','#e5e5e0')});},<br />
function () {<br />
$(this).removeClass("collapse").addClass("expand");<br />
$(this).parent().find('div:first').css('display','none');<br />
$(this).hover(<br />
function (){$(this).css('background-color','#F5F5F0')},<br />
function (){$(this).css('background-color','#FFFFFF')});}<br />
).addClass('expand');<br />
}<br />
<br />
function ddmozilla() {<br />
ddtoggle();<br />
}<br />
<br />
$(function () {<br />
ddnav();<br />
ddnavsub();<br />
<br />
if($.browser.mozilla) {ddmozilla();}<br />
});<br />
</script><br />
<center><br />
<div id="header"><br />
<img src="https://static.igem.org/mediawiki/2009/0/04/UofA_biobytes_HeaderBanner5.png" width="965" height="235"><br />
<br />
<!-- logo --><br />
<div id="logo"><br />
</div><br />
<!-- END logo --><br />
<br />
<!-- main navigation --><br />
<div id="mainNav"><br />
<ul id="ddNav"><br />
<br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta">&nbsp; Home &nbsp; </a><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/Team">&nbsp; Team &nbsp; </a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Team">Undergraduates</a><br />
<a href="https://2009.igem.org/Team:Alberta/Team#Team_Ad">Team Advisors</a><br />
<a href="https://2009.igem.org/Team:Alberta/Team#Fac_Con">Faculty Consultants</a><br />
</div><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta">&nbsp;Project&nbsp;</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Project/assemblyoverview">DNA Assembly</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/AutomationOverview">Automation</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Bioinformatics">Organism Design</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Chromosome_Assembly">Chromosome Assembly</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Outreach">Human Practices</a><br />
</div><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/MedalRequirements">&nbsp;Achievements&nbsp;</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/MedalRequirements">Medals & Prizes</a><br />
<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Alberta" target="_blank">Parts Registry</a><br />
<br />
</div><br />
</li><br />
<li><br />
<a>&nbsp; &nbsp; Lab &nbsp; &nbsp; </a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Calendar">Lab Notebook</a><br />
<a href="https://2009.igem.org/Team:Alberta/Blackboard">Lab Blackboard</a><br />
<a href="https://2009.igem.org/Team:Alberta/Protocols">Protocols</a><br />
<br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/References/Keyword">&nbsp;References&nbsp;</a><br />
</li><br />
<br />
</ul><br />
<br />
</div><br />
<br />
<!-- END main navigation --><br />
<br />
</div><br />
</center><br />
</html><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br />
{{:Team:Alberta/LeftBar}}<br />
<br />
<html><br />
<style type="text/css"><br />
body {background-color:#f8f8f8;}<br />
</style><br />
</html></div>
Wiltshir
http://2009.igem.org/Team:Alberta/Team
Team:Alberta/Team
2009-10-21T07:49:07Z
<p>Wiltshir: </p>
<hr />
<div>{{:Team:Alberta/Template3}}<br />
<br />
<html><br />
<head><br />
<style type="text/css"><br />
.b1f, .b2f, .b3f, .b4f{font-size:1px; overflow:hidden; display:block;}<br />
.b1f {height:1px; background:#e1e1e1; margin:0 5px;}<br />
.b2f {height:1px; background:#e1e1e1; margin:0 3px;}<br />
.b3f {height:1px; background:#e1e1e1; margin:0 2px;}<br />
.b4f {height:2px; background:#e1e1e1; margin:0 1px;}<br />
.content {background: #e1e1e1;}<br />
.content div {margin-left: 5px;}<br />
</style><br />
</head><br />
<br />
<div class="all"><br />
<div style="background:#FFFFFF"><br />
<br />
<!-- adjust table width, main background and padding between cells and edge of background --><br />
<br />
<br />
<table width=60% style="background:#FFFFFF; padding:2px;"><br />
<br />
<tr><br />
<td style="height: 400; padding-left: 10px; padding-right: 10px; padding-top: 11px;"><br />
<b class="b1f"></b><b class="b2f"></b><b class="b3f"></b><b class="b4f"></b><br />
<div class="Recoli"><br />
<div style="height: 400; background:#FFFFFF; colorou line-height:100% padding: 3px 0px;"><br />
<h1>Project BioBytes</h1><br />
<br />
<!-- <div align="justify" style="padding-left:20px; padding-right:20px"> --><br />
<div align="justify"><br />
<br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/2009/8/8f/UofAiGEM2009_groupphoto_IMG_5009.JPG" width="320" height="407"><br />
</center><br />
<br />
<br> <br />
<br />
<table><br />
<br />
<tr><br />
<td><br />
<div align="left"><strong>Kalon Armstrong</strong> <br><br />
<em>Molecular Genetics</em> <br><br />
</div><br />
<td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/3/34/UofA09_team_Kalon_Armstrong.jpg" hspace="20"><br />
</td><br />
<td><br />
<div align="justify" >I've recently completed my BSc in Molecular Genetics and will be entering the Engineering program in the fall of 2009. Most of my growing-up took place in the small town of Cochrane, Alberta. My ultimate goals consist of working in the Biotechnology or health care industry. While my interest in music, movies, snowboarding, and hanging out with friends may seem stereotypical on the surface, they feel unique in their own right and keep me busy most of the time. This will be my first year on the U of A iGEM team and I am excited to help take the competition to a new level. I think that iGEM will be a great experience because it demands innovation and collaboration on levels rarely seen in undergraduate programs.</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td><br />
<tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Eric Bennett</strong><br><br />
<em>Electrical Biomedical Engineering</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/e/ef/UofA09_Eric_Bennett.JPG" hspace="20"><br />
</td><br />
<br />
<td><br />
<div align="justify" >I am entering my final year of engineering at the U of A. After graduation, I hope to do research either with a biotechnology company or in grad school. I think that iGEM is a great way to gain valuable experience and is an effective way of accelerating the field of synthetic biology. My interests include brain-machine interfacing, genetic engineering, and robotic control systems. My hobbies include playing guitar, video games, the occasional sport, reading, and fixing my car.</div><br />
</td><br />
</tr> <br />
<br />
<tr><br />
<td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Max Buchko</strong><br><br />
<em>Honors Biochemistry</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="http://igem.biochem.ualberta.ca/wiki/images/3/3d/UofA_iGEM09_Max_Pic1.png" ALIGN="LEFT" hspace="20"><br />
</td><br />
<br />
<td><br />
<div align="justify" style="padding-right:20px">I am in my third year of Honors Biochemistry and wish to pursue a career in medicine. In my time away from the lab I enjoy a wide variety of sports including soccer, boxing, and rifle silhouette shooting. I have also been known to strum a chord or two at an intolerable volume to the annoyance of the people living upstairs.<br />
I hope for the best with iGEM at MIT in 2009. This competition is a means of proving yourself at an exemplary level amongst many of the top international minds, and it is this challenge that I look forward to the most.</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Oscar Cortés</strong><br><br />
<em>Bsc. Specialization molecular genetics</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/7/77/Oscar.jpg" ALIGN="LEFT" hspace="20"><br />
</td><br />
<td><br />
<div align="justify" style="padding-right:20px">My future plans include entering into a Masters program in Medical Genetics or Human Genetics, and pursuing this discipline towards a PhD. I enjoy reading books about the human genome and advancements in stem cell research. In my spare time I like to play a variety of sports that I am not necessarily good at: soccer, softball, and dodge ball. I see iGEM as an extraordinary opportunity for me to be exposed to real life research, in which my knowledge of molecular genetics will be challenged, and will help me further my understanding of Synthetic Biology.<br />
"Man with all his noble qualities still bears in his bodily frame the indelible stamp of his lowly origin"- Charles Darwin</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td><br />
</tr> <br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Anh Dao</strong><br><br />
<em>Biological Sciences and Chemistry</em><br> <br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/c/cf/UofA09_team_Ahn_Dao.jpg" ALIGN="LEFT" hspace="20"><br />
</td><br />
<br />
<td><br />
<div align="justify" style="padding-right:20px">I am interested in a research career after I finish my degree. I am leaning towards the field of Microbiology to study the many micro-organisms that have not yet been discovered. However, I may enroll in graduate studies after my undergraduate degree to expand my knowledge and gain more experience in the laboratory. Being in a competitive team and atmosphere is a motivating and exciting opportunity that I do not want to miss out on. By creating the smallest artificial E. coli genome we can extend future research. We are attempting to understand and standardize the E. coli genome so that this methodology can be applied to more complex model organisms.</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Uchechukwu Davidson</strong><br><br />
<em>Honors biochemistry</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/d/d5/Uche.jpg" ALIGN="LEFT" hspace="20"><br />
</td><br />
<td><br />
<div align="justify" style="padding-right:20px">I am in my third year of Biochemistry at the University of Alberta. I wish to pursue a career in medicine after my degree. I enjoy sports, movies, and music at my time away from my studies. The iGEM provides an opportunity to experience creativity, innovation, and ingenuity which are sometimes absent at the undergraduate level of science.</div><br />
</td><br />
</tr><br />
<br />
<tr> <br />
<td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Youness Elkhalidy</strong><br><br />
<em>Honors immunology and infection</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/f/fe/UofAiGEM2009_Youness_IMG_5049.JPG" width="200" height="300" ALIGN="LEFT" hspace="20"><br />
</td><br />
<td><br />
I am a first year student at the University of Alberta. I hope to enter medical school in the near future. I am currently taking part in mitotic-spindle regulation research from a genetics perspective. I have a passion for science and enjoy sports such as basketball and soccer. iGEM is an great learning experience not only in the cutting-edge field of Synthetic Biology but also in leadership and business management. I will enjoy taking part in research that combines many fields of science as well as socialize with many of my team members who share common interests.<br />
</td><br />
</tr><br />
<br />
<tr><br />
<td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Justin Fedor</strong><br><br />
<em>Honours Biochemistry</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/0/0d/IMG_4985.JPG" ALIGN="LEFT" hspace="20"><br />
</td><br />
<br />
<td><br />
<div align="justify" style="padding-right:20px">I have completed my undergraduate degree in Biochemistry this year and have began my PhD studies this September. I play piano and am attempting to learn the cello. My nerdy tendencies are obvious when I say that I like Star Trek TNG but not DS9. Last summer I worked in the biomedical research lab of Dr. Larry Unsworth of the National Institute for Nanotechnology (NINT), which has further piqued my interest in the field of nanotechnology. In the hopefully not too distant future I plan on becoming a researcher studying the mechanisms of membrane bound enzymes, particularly oxidoreductases. iGEM has proved a fantastic experience, it has served to mature me further as a scientist.</div><br />
</td><br />
</tr><br />
<br />
<tr><td colspan = "2"> <br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Jason Gardiner</strong> <br><br />
<em>BSc. Specialization in Botany</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/2/2b/UofA09_team_Jason_Gardiner.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">Jason is a veteran of iGEM 2007, where his team "The Butanerds" won first place in the Energy track. His hobbies include Softball, Beach volleyball, soccer, as well as playing the guitar and fiddling with the iGEM 2009 Wiki. Jason has applied to continue his education in graduate studies at the University of Alberta in 2010. His degree in Botany focuses mostly on the molecular side of plants and he hopes to use this knowledge applying Synthetic Biology to plants. One day he hopes to solve all of the world's problems using plants and Synthetic Biology.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><br />
<div align="left"><br />
<strong>Erin Garside</strong> <br><br />
<em>Biological Sciences</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/6/60/UofA09_team_Erin_Garside.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I completed my BSc. degree this year and plan to do graduate work in biochemistry. After that - who knows? I think iGEM will be a great experience, and that it's about time Synthetic Biology really took off. In my spare time I like to read, play computer games (especially the Sims) and raise cats.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Boris Henriquez</strong><br><br />
<em>Biological Sciences</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/e/ec/UofA09_team_Boris_Heneriquez.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I have just completed the third year of my BSc. degree. My favorite classes so far have been in the fields of Biochemistry and Genetics. I am most interested in human development and embryogenesis and hope to learn more about these topics in my fourth year. My ultimate goal is to have a career in the medical industry as both a physician and as a researcher. When I'm not busy with school or work my usual activities are pretty standard. I like to hang out with friends and family and I crave the outdoors. This is my first year with iGEM and I'm really excited to learn more about Synthetic Biology. </div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Stephen Jahns</strong><br><br />
<em>Molecular Genetics</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/a/a8/Alberta_steve.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I am in my second year at the University of Alberta. After taking a year of engineering, I decided to transfer to molecular genetics in order to fulfill my childhood desire of creating an army of giant bat-rabbits. I plan on going on through to graduate school to earn a PhD in order to reach my goals. In my spare time I like to run around, ride my bike, play music, cook delicious food, and pretty much all the other fun things kids are doing these days. In my first year I helped to build a car with the U of A's Formula SAE fabrication team. I had a good time building stuff out of metal, and this year I know that I'm going to have a blast building (or at least attempting to build) a novel organism out of DNA parts.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<br />
<tr><td><div align="left"><br />
<strong>Eric Leung</strong> <br><br />
<em>Honors Pharmacology</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/c/c7/Alberta_Eric_Leung.JPG" ALIGN="LEFT" hspace="20"><br />
<br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I am currently in my final year of an Honors Pharmacology degree. What I do after this degree is up in the air but it is definitely something in the health sciences. In my spare time, I like to play basketball, badminton, run, and catch up on TV shows. Soon I hope I can become a professional baker in my free time. Traveling to Europe, Mexico, and Japan is also in the books somewhere along the way.</div><br />
<br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<br />
<tr><td><div align="left"><br />
<strong>David Lloyd</strong><br> <br />
<em> Biochemistry</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/6/6f/UofA09_team_David_Lloyd.jpg" ALIGN="LEFT" hspace="20"><br />
<br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I am a fourth year student, studying at the University of Alberta. While Biochemistry is my major, I have many interests including Genetics, Immunology, Cell Biology, Bioinformatics, and Microbiology. In my spare time I can be found playing sports like soccer, volleyball, or squash, as well as playing piano, listening to music, and playing video games. In the future, I hope to continue into a graduate program in Biochemistry, Cell Biology, Synthetic Biology, or in another field. iGEM is of great interest to myself because of its application to the future of Synthetic Biology. It is an awesome challenge which will hopefully help me to cultivate myself.</div><br />
<br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Enoch Ng</strong><br><br />
<em>Biological Sciences/Business</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/7/7c/UofA09_team_Enoch_Ng.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I am entering my fourth year of studies at the University of Alberta, and am looking forward to the challenge that iGEM provides. In the future I hope to travel across the world and meet people ranging from Meshaal to Obama and remain a glorified generalist. </div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Emera Nguyen</strong><br><br />
<em>BSc. Biological Sciences/Economics</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/5/52/UofA09_team_Emera_Nguyen.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I am a BSc student entering my fourth year in a Biological Sciences major and Economics minor. This year will be my second year on an iGEM team, and my first year representing the U of A team. In my downtime, things I enjoy include spending quality time with friends and family and traveling to lesser-known parts of the world. One year's experience later, the iGEM competition still impresses me with the unique opportunities it provides for student growth. This research competition is one of the highlights of my undergraduate experience.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr> <br />
<br />
<br />
<tr><td><div align="left"><br />
<strong>Mitch Paquette</strong><br><br />
<em>BSc. Honors Molecular Genetics</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/1/12/IMG_5052.jpg" width="200" height="300" ALIGN="LEFT" hspace="20"><br />
<br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I have transfered into the honors program in Molecular Genetics from a BSc General program in Physical Sciences. After my degree is completed I plan to apply to graduate studies in Molecular Biology, after which I hope to work in research or academia. What excites me most about iGEM is the opportunity to gain research experience in such a new field.</div><br />
</td></tr> <br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Amber Paul</strong><br><br />
<em>BSc. Specialization Immunology and Infection</em><br><br />
</div></td></tr><br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/5/5f/IMG_4987.jpg" width="200" height="300" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I plan to find the cure for cancer. Seriously. Will be attending graduate school to earn a PhD following my undergraduate degree. I would like to live somewhere warm that allows me to do research, preferably Maui. iGEM is a developmental forefront to Synthetic Biology, something that I love to be part of. Its challenging and fun. To be able to determine the minimal genes for a simple prokaryote, we provide a scaffold to future research on larger, more complex cells such as cancerous tissues, simple eukaryotes, etc.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Julia Pon</strong><br><br />
<em>Honors Molecular Genetics</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/e/ec/UofA_Julia_Pon_photo.JPG" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">My future plans include pursuing PhD in Medical Genetics. I've worked in biological research labs for the past two summers, with last summer spent at the German Cancer Research Institute in Heidelberg, Germany. I'll be working in both the iGEM lab and a Medical Genetics research lab during summer 2009. My heritage is a mixture of English, Chinese, and Danish. iGEM helps move biology to a streamlined, standardized, abstracted process that opens the door to interdisciplinary collaborations and new applications. I'm excited about the many applications of Synthetic Biology and am enjoying the student-directed and team-oriented nature of iGEM.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<br />
<tr><td><div align="left"> <br />
<strong>Alina Ponomarev</strong><br><br />
<em>Biological Sciences</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/d/d6/UofAiGEM2009_Alina_IMG_5023.JPG" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I am currently a second year student in Biological Sciences and I am considering transferring into the Immunology and Infection program for my third year. I joined iGEM at the end of my first year and can honestly say that it has been an unbeatable learning experience. It is very exciting to think that the progress we have made can positively impact synthetic biology and society in general. In the future, my career goal is to become a Pediatrician. However, after doing labwork at the iGEM lab this summer, I have begun to consider a career in medical research. </div> <br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Kelly Robinson</strong><br><br />
<em>Hon. Biochemistry</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/9/92/UofA09_team_Kelly_Robinson.png" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I completed my BSc. in Biochemistry this year and have enrolled in the University of Alberta's Chemical Engineering program in hopes of fusing the fundamental science of biological machines with the entrepreneurial world of engineering. I got involved in iGEM a couple of years ago when I went to a presentation put on by one of the past teams. I ended up applying in the middle of the night on the last day of registration. That said, it has been a great experience ever since. I'm glad that this year I get the opportunity to do some full time work and really get into the project. Overall, iGEM has a lot to offer anyone who gets involved (including incorrigible advisors: you know who you are!).</div><br />
<br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr> <br />
<br />
<tr><td><div align="left"><br />
<strong>James Rodway</strong><br><br />
<em>Electrical Engineering</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/a/a5/UofA09_team_James_Rodway.jpg" ALIGN="LEFT" hspace="20"><br />
<br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I am currently finishing up a co-op Electrical Engineering degree, and am aiming to do some graduate work in more of the computer area, specifically modeling. At the University, I've been involved with the ARVP and iGEM.<br />
I haven't really had much time for hobbies in a while, but when I did I played more video games, the guitar, and I actually read books for entertainment value. During my time on last year's iGEM team I found that it was a pretty cool multidisciplinary project, which drew in a few different disciplines that I have never really interacted with previously. It was very impressive to see what we, and all the iGEM teams, had accomplished by the end of last year's competition.</div><br />
<br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Andy Spencer</strong><br><br />
<em>Honours Biochemistry</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/c/ca/UofA09_team_Andy_Spencer.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">In 5 years I see myself on one of two different paths, medicine or oncology research. I grew up in the Okanagan, British Columbia, and love spending my summers at the beach . In my spare time I enjoy playing squash and bouldering. iGEM to me represents a fundamental value of science as a discipline; that people can from diverse backgrounds with a common interest and curiosity can work as a cohesive team to overcome barriers and problems. </div><br />
<br />
</td></tr> <br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Jonathan Tam</strong><br><br />
<em>Honors Cell Biotechnology</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/e/e2/UofA09_team_Jonathan_Tam.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I have completed my Bachelor's degree in Honors Cell Biotechnology and intend to pursue a medical degree in Germany in the near future. I am very interested in the fields of Molecular Biology and Immunology. For the last two years, I have been keeping myself busy studying the development of macrophages in the lab of Dr. Daniel Barreda. Outside of the lab, I am an avid photographer and downhill mountain biker. The iGEM competition accelerates the development of Synthetic Biology as a field. To be a part of our University of Alberta team is an excellent opportunity for collaboration and advancement.</div><br />
</td></tr> <br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Jennifer Yau</strong><br><br />
<em>Honors Biochemistry</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/9/99/UofAiGEM2009_Jen_IMG_5048.JPG" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I am currently in the second year of my undergraduate program with the ultimate goal of attending graduate school to pursue a research career in Geriatric Medicine. Additional science related activities of mine include my interest in astronomy and promoting science to elementary students through volunteer work. Otherwise, I dedicate the majority of my free time to the arts of chainmaille, knitting, and jazz/classical piano. This is my first year on iGEM and I am ecstatic to be partaking in a project that requires such diverse disciplinary backgrounds. Not only will this be a great learning experience in so many perspectives, but also an opportunity to contribute significant ideas to the ongoing research in Synthetic Biology</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr> <br />
<br />
<tr><td><div align="left"><br />
<strong>Zach Wiltshire</strong> <br><br />
<em>BSc. Specialization in Cell Biology</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/0/0b/UofA09_team_Zach_Wiltshire2.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I am entering into my fourth year in the Cell Biology program at the U of A and have already experienced iGEM once before as a member of the 2008 National Institute for Nanotechnology team. Through my degree I have found myself to have interests ranging from immunology to both eukaryotic & prokaryotic cellular anatomy & physiology. I also happen to be very fond of molecules that fluoresce. My experience with iGEM last year opened my eyes to the possibilities which engineering biological systems can offer. Over the course of the 2009 competition I hope to have just as many opportunities to learn as I did last year.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<tr><td><br />
<a name="Team_Ad"></a><br />
<strong>The Supervisors</strong><br />
</tr><td><br />
<br />
<tr><td><br />
<strong>Mike Ellison</strong><br />
<img src="https://static.igem.org/mediawiki/2009/d/d5/UofA09_teamad_Mike_Ellison.jpg" ALIGN="LEFT" hspace="20"><br />
</td></tr><br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><br />
<br />
<strong>Doug Ridgway</strong><br />
<img src="https://static.igem.org/mediawiki/2009/c/cc/UofA09_teamad_Douglas_Ridgway.jpg" ALIGN="LEFT" hspace="20"></a><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><br />
<strong>James Maclagan</strong><br />
<img src="https://static.igem.org/mediawiki/2009/a/ab/UofAigem2009_JamesMIMG_4975.JPG" ALIGN="LEFT" hspace="20"></a><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</tr></td><br />
<tr><td><br />
<a name="Fac_Con"></a><br />
<strong>Faculty Consultants</strong><br />
</tr><td><br />
<br />
<tr><td><br />
<strong>Chris Backhouse</strong><br />
<br><br />
Department of Electrical Engineering<br />
<br><br />
christopher.backhouse@ualberta.ca<br />
<img src="https://static.igem.org/mediawiki/2009/5/54/UofAigem2009_Consultant_Backhouse.jpg" ALIGN="LEFT" hspace="20"><br />
</td><br />
<br />
<td><br />
<div align="justify" ><br />
Nanobiotechnologies give us the ability to manipulate and sense at the level of individual molecules, with a tremendous potential impact on both human health and the economy. To a large extent, this potential is likely to be realised through the development of Lab on Chip (LOC) technologies. Although the LOC technologies are powerful, the complexity of the infrastructure required to support LOC operation has hindered the widespread adoption of LOC methods in life science applications. A central theme in the work of the Backhouse lab (<a href="http://www.ece.ualberta.ca/~aml/">the Applied Miniaturisation Lab, AML</a>) is the development of extremely inexpensive (e.g. $1000) systems for implementing nanobiotechnologies/molecular biology, especially for medical diagnostic applications.</div><br />
</td><br />
</tr><br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><br />
<br />
<strong>Robert Campbell</strong><br />
<br><br />
Department of Chemistry<br />
<br><br />
robert.e.campbell@ualberta.ca<br />
<img src="https://static.igem.org/mediawiki/2009/2/25/UofAigem2009_Consultant_Campbell.jpg" width=200 height=274.19355 ALIGN="LEFT" hspace="20"></a><br />
</td><br />
<br />
<td><br />
<div align="justify" ><br />
Robert E. Campbell is an associate professor and Canada Research Chair in Bioanalytical Chemistry in the Department of Chemistry of the University of Alberta. Research in his laboratory, (<a href="http://www.chem.ualberta.ca/~campbell/">the Campbell Research Group</a>), is focused on protein engineering and the development of new fluorescent protein variants for construction of FRET-based biosensors.<br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><br />
<strong>Linda Reha-Krantz</strong><br />
<br><br />
Department of Biological Sciences<br />
<br><br />
Linda.Reha-Krantz@ualberta.ca<br />
<img src="https://static.igem.org/mediawiki/2009/4/40/UofAigem2009_Consultant_Reha-Krantz.jpg" width=200 height=300 ALIGN="LEFT" hspace="20"></a><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</tr></td><br />
<br />
<tr><td><br />
<strong>Tracy Raivio</strong><br />
<br><br />
Department of Biological Sciences<br />
<br><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</tr></td><br />
<br />
<tr><td><br />
<strong>Jon Dennis</strong><br />
<br><br />
Department of Biological Sciences<br />
<br><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</tr></td><br />
<br />
<br />
<br />
<br />
</table><br />
<br />
</div><br />
<br />
</div></div><br />
<b class="b4f"></b><b class="b3f"></b><b class="b2f"></b><b class="b1f"></b><br />
</td><br />
</tr><br />
<br />
</table><br />
</html></div>
Wiltshir
http://2009.igem.org/File:UofAigem2009_Consultant_Reha-Krantz.jpg
File:UofAigem2009 Consultant Reha-Krantz.jpg
2009-10-21T07:48:53Z
<p>Wiltshir: </p>
<hr />
<div></div>
Wiltshir
http://2009.igem.org/File:UofAigem2009_Consultant_Campbell.jpg
File:UofAigem2009 Consultant Campbell.jpg
2009-10-21T07:47:05Z
<p>Wiltshir: </p>
<hr />
<div></div>
Wiltshir
http://2009.igem.org/File:UofAigem2009_Consultant_Backhouse.jpg
File:UofAigem2009 Consultant Backhouse.jpg
2009-10-21T07:44:38Z
<p>Wiltshir: </p>
<hr />
<div></div>
Wiltshir
http://2009.igem.org/Team:Alberta/Team
Team:Alberta/Team
2009-10-21T07:41:34Z
<p>Wiltshir: </p>
<hr />
<div>{{:Team:Alberta/Template3}}<br />
<br />
<html><br />
<head><br />
<style type="text/css"><br />
.b1f, .b2f, .b3f, .b4f{font-size:1px; overflow:hidden; display:block;}<br />
.b1f {height:1px; background:#e1e1e1; margin:0 5px;}<br />
.b2f {height:1px; background:#e1e1e1; margin:0 3px;}<br />
.b3f {height:1px; background:#e1e1e1; margin:0 2px;}<br />
.b4f {height:2px; background:#e1e1e1; margin:0 1px;}<br />
.content {background: #e1e1e1;}<br />
.content div {margin-left: 5px;}<br />
</style><br />
</head><br />
<br />
<div class="all"><br />
<div style="background:#FFFFFF"><br />
<br />
<!-- adjust table width, main background and padding between cells and edge of background --><br />
<br />
<br />
<table width=60% style="background:#FFFFFF; padding:2px;"><br />
<br />
<tr><br />
<td style="height: 400; padding-left: 10px; padding-right: 10px; padding-top: 11px;"><br />
<b class="b1f"></b><b class="b2f"></b><b class="b3f"></b><b class="b4f"></b><br />
<div class="Recoli"><br />
<div style="height: 400; background:#FFFFFF; colorou line-height:100% padding: 3px 0px;"><br />
<h1>Project BioBytes</h1><br />
<br />
<!-- <div align="justify" style="padding-left:20px; padding-right:20px"> --><br />
<div align="justify"><br />
<br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/2009/8/8f/UofAiGEM2009_groupphoto_IMG_5009.JPG" width="320" height="407"><br />
</center><br />
<br />
<br> <br />
<br />
<table><br />
<br />
<tr><br />
<td><br />
<div align="left"><strong>Kalon Armstrong</strong> <br><br />
<em>Molecular Genetics</em> <br><br />
</div><br />
<td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/3/34/UofA09_team_Kalon_Armstrong.jpg" hspace="20"><br />
</td><br />
<td><br />
<div align="justify" >I've recently completed my BSc in Molecular Genetics and will be entering the Engineering program in the fall of 2009. Most of my growing-up took place in the small town of Cochrane, Alberta. My ultimate goals consist of working in the Biotechnology or health care industry. While my interest in music, movies, snowboarding, and hanging out with friends may seem stereotypical on the surface, they feel unique in their own right and keep me busy most of the time. This will be my first year on the U of A iGEM team and I am excited to help take the competition to a new level. I think that iGEM will be a great experience because it demands innovation and collaboration on levels rarely seen in undergraduate programs.</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td><br />
<tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Eric Bennett</strong><br><br />
<em>Electrical Biomedical Engineering</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/e/ef/UofA09_Eric_Bennett.JPG" hspace="20"><br />
</td><br />
<br />
<td><br />
<div align="justify" >I am entering my final year of engineering at the U of A. After graduation, I hope to do research either with a biotechnology company or in grad school. I think that iGEM is a great way to gain valuable experience and is an effective way of accelerating the field of synthetic biology. My interests include brain-machine interfacing, genetic engineering, and robotic control systems. My hobbies include playing guitar, video games, the occasional sport, reading, and fixing my car.</div><br />
</td><br />
</tr> <br />
<br />
<tr><br />
<td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Max Buchko</strong><br><br />
<em>Honors Biochemistry</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="http://igem.biochem.ualberta.ca/wiki/images/3/3d/UofA_iGEM09_Max_Pic1.png" ALIGN="LEFT" hspace="20"><br />
</td><br />
<br />
<td><br />
<div align="justify" style="padding-right:20px">I am in my third year of Honors Biochemistry and wish to pursue a career in medicine. In my time away from the lab I enjoy a wide variety of sports including soccer, boxing, and rifle silhouette shooting. I have also been known to strum a chord or two at an intolerable volume to the annoyance of the people living upstairs.<br />
I hope for the best with iGEM at MIT in 2009. This competition is a means of proving yourself at an exemplary level amongst many of the top international minds, and it is this challenge that I look forward to the most.</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Oscar Cortés</strong><br><br />
<em>Bsc. Specialization molecular genetics</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/7/77/Oscar.jpg" ALIGN="LEFT" hspace="20"><br />
</td><br />
<td><br />
<div align="justify" style="padding-right:20px">My future plans include entering into a Masters program in Medical Genetics or Human Genetics, and pursuing this discipline towards a PhD. I enjoy reading books about the human genome and advancements in stem cell research. In my spare time I like to play a variety of sports that I am not necessarily good at: soccer, softball, and dodge ball. I see iGEM as an extraordinary opportunity for me to be exposed to real life research, in which my knowledge of molecular genetics will be challenged, and will help me further my understanding of Synthetic Biology.<br />
"Man with all his noble qualities still bears in his bodily frame the indelible stamp of his lowly origin"- Charles Darwin</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td><br />
</tr> <br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Anh Dao</strong><br><br />
<em>Biological Sciences and Chemistry</em><br> <br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/c/cf/UofA09_team_Ahn_Dao.jpg" ALIGN="LEFT" hspace="20"><br />
</td><br />
<br />
<td><br />
<div align="justify" style="padding-right:20px">I am interested in a research career after I finish my degree. I am leaning towards the field of Microbiology to study the many micro-organisms that have not yet been discovered. However, I may enroll in graduate studies after my undergraduate degree to expand my knowledge and gain more experience in the laboratory. Being in a competitive team and atmosphere is a motivating and exciting opportunity that I do not want to miss out on. By creating the smallest artificial E. coli genome we can extend future research. We are attempting to understand and standardize the E. coli genome so that this methodology can be applied to more complex model organisms.</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Uchechukwu Davidson</strong><br><br />
<em>Honors biochemistry</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/d/d5/Uche.jpg" ALIGN="LEFT" hspace="20"><br />
</td><br />
<td><br />
<div align="justify" style="padding-right:20px">I am in my third year of Biochemistry at the University of Alberta. I wish to pursue a career in medicine after my degree. I enjoy sports, movies, and music at my time away from my studies. The iGEM provides an opportunity to experience creativity, innovation, and ingenuity which are sometimes absent at the undergraduate level of science.</div><br />
</td><br />
</tr><br />
<br />
<tr> <br />
<td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Youness Elkhalidy</strong><br><br />
<em>Honors immunology and infection</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/f/fe/UofAiGEM2009_Youness_IMG_5049.JPG" width="200" height="300" ALIGN="LEFT" hspace="20"><br />
</td><br />
<td><br />
I am a first year student at the University of Alberta. I hope to enter medical school in the near future. I am currently taking part in mitotic-spindle regulation research from a genetics perspective. I have a passion for science and enjoy sports such as basketball and soccer. iGEM is an great learning experience not only in the cutting-edge field of Synthetic Biology but also in leadership and business management. I will enjoy taking part in research that combines many fields of science as well as socialize with many of my team members who share common interests.<br />
</td><br />
</tr><br />
<br />
<tr><br />
<td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Justin Fedor</strong><br><br />
<em>Honours Biochemistry</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/0/0d/IMG_4985.JPG" ALIGN="LEFT" hspace="20"><br />
</td><br />
<br />
<td><br />
<div align="justify" style="padding-right:20px">I have completed my undergraduate degree in Biochemistry this year and have began my PhD studies this September. I play piano and am attempting to learn the cello. My nerdy tendencies are obvious when I say that I like Star Trek TNG but not DS9. Last summer I worked in the biomedical research lab of Dr. Larry Unsworth of the National Institute for Nanotechnology (NINT), which has further piqued my interest in the field of nanotechnology. In the hopefully not too distant future I plan on becoming a researcher studying the mechanisms of membrane bound enzymes, particularly oxidoreductases. iGEM has proved a fantastic experience, it has served to mature me further as a scientist.</div><br />
</td><br />
</tr><br />
<br />
<tr><td colspan = "2"> <br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Jason Gardiner</strong> <br><br />
<em>BSc. Specialization in Botany</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/2/2b/UofA09_team_Jason_Gardiner.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">Jason is a veteran of iGEM 2007, where his team "The Butanerds" won first place in the Energy track. His hobbies include Softball, Beach volleyball, soccer, as well as playing the guitar and fiddling with the iGEM 2009 Wiki. Jason has applied to continue his education in graduate studies at the University of Alberta in 2010. His degree in Botany focuses mostly on the molecular side of plants and he hopes to use this knowledge applying Synthetic Biology to plants. One day he hopes to solve all of the world's problems using plants and Synthetic Biology.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><br />
<div align="left"><br />
<strong>Erin Garside</strong> <br><br />
<em>Biological Sciences</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/6/60/UofA09_team_Erin_Garside.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I completed my BSc. degree this year and plan to do graduate work in biochemistry. After that - who knows? I think iGEM will be a great experience, and that it's about time Synthetic Biology really took off. In my spare time I like to read, play computer games (especially the Sims) and raise cats.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Boris Henriquez</strong><br><br />
<em>Biological Sciences</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/e/ec/UofA09_team_Boris_Heneriquez.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I have just completed the third year of my BSc. degree. My favorite classes so far have been in the fields of Biochemistry and Genetics. I am most interested in human development and embryogenesis and hope to learn more about these topics in my fourth year. My ultimate goal is to have a career in the medical industry as both a physician and as a researcher. When I'm not busy with school or work my usual activities are pretty standard. I like to hang out with friends and family and I crave the outdoors. This is my first year with iGEM and I'm really excited to learn more about Synthetic Biology. </div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Stephen Jahns</strong><br><br />
<em>Molecular Genetics</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/a/a8/Alberta_steve.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I am in my second year at the University of Alberta. After taking a year of engineering, I decided to transfer to molecular genetics in order to fulfill my childhood desire of creating an army of giant bat-rabbits. I plan on going on through to graduate school to earn a PhD in order to reach my goals. In my spare time I like to run around, ride my bike, play music, cook delicious food, and pretty much all the other fun things kids are doing these days. In my first year I helped to build a car with the U of A's Formula SAE fabrication team. I had a good time building stuff out of metal, and this year I know that I'm going to have a blast building (or at least attempting to build) a novel organism out of DNA parts.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<br />
<tr><td><div align="left"><br />
<strong>Eric Leung</strong> <br><br />
<em>Honors Pharmacology</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/c/c7/Alberta_Eric_Leung.JPG" ALIGN="LEFT" hspace="20"><br />
<br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I am currently in my final year of an Honors Pharmacology degree. What I do after this degree is up in the air but it is definitely something in the health sciences. In my spare time, I like to play basketball, badminton, run, and catch up on TV shows. Soon I hope I can become a professional baker in my free time. Traveling to Europe, Mexico, and Japan is also in the books somewhere along the way.</div><br />
<br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<br />
<tr><td><div align="left"><br />
<strong>David Lloyd</strong><br> <br />
<em> Biochemistry</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/6/6f/UofA09_team_David_Lloyd.jpg" ALIGN="LEFT" hspace="20"><br />
<br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I am a fourth year student, studying at the University of Alberta. While Biochemistry is my major, I have many interests including Genetics, Immunology, Cell Biology, Bioinformatics, and Microbiology. In my spare time I can be found playing sports like soccer, volleyball, or squash, as well as playing piano, listening to music, and playing video games. In the future, I hope to continue into a graduate program in Biochemistry, Cell Biology, Synthetic Biology, or in another field. iGEM is of great interest to myself because of its application to the future of Synthetic Biology. It is an awesome challenge which will hopefully help me to cultivate myself.</div><br />
<br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Enoch Ng</strong><br><br />
<em>Biological Sciences/Business</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/7/7c/UofA09_team_Enoch_Ng.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I am entering my fourth year of studies at the University of Alberta, and am looking forward to the challenge that iGEM provides. In the future I hope to travel across the world and meet people ranging from Meshaal to Obama and remain a glorified generalist. </div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Emera Nguyen</strong><br><br />
<em>BSc. Biological Sciences/Economics</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/5/52/UofA09_team_Emera_Nguyen.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I am a BSc student entering my fourth year in a Biological Sciences major and Economics minor. This year will be my second year on an iGEM team, and my first year representing the U of A team. In my downtime, things I enjoy include spending quality time with friends and family and traveling to lesser-known parts of the world. One year's experience later, the iGEM competition still impresses me with the unique opportunities it provides for student growth. This research competition is one of the highlights of my undergraduate experience.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr> <br />
<br />
<br />
<tr><td><div align="left"><br />
<strong>Mitch Paquette</strong><br><br />
<em>BSc. Honors Molecular Genetics</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/1/12/IMG_5052.jpg" width="200" height="300" ALIGN="LEFT" hspace="20"><br />
<br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I have transfered into the honors program in Molecular Genetics from a BSc General program in Physical Sciences. After my degree is completed I plan to apply to graduate studies in Molecular Biology, after which I hope to work in research or academia. What excites me most about iGEM is the opportunity to gain research experience in such a new field.</div><br />
</td></tr> <br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Amber Paul</strong><br><br />
<em>BSc. Specialization Immunology and Infection</em><br><br />
</div></td></tr><br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/5/5f/IMG_4987.jpg" width="200" height="300" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I plan to find the cure for cancer. Seriously. Will be attending graduate school to earn a PhD following my undergraduate degree. I would like to live somewhere warm that allows me to do research, preferably Maui. iGEM is a developmental forefront to Synthetic Biology, something that I love to be part of. Its challenging and fun. To be able to determine the minimal genes for a simple prokaryote, we provide a scaffold to future research on larger, more complex cells such as cancerous tissues, simple eukaryotes, etc.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Julia Pon</strong><br><br />
<em>Honors Molecular Genetics</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/e/ec/UofA_Julia_Pon_photo.JPG" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">My future plans include pursuing PhD in Medical Genetics. I've worked in biological research labs for the past two summers, with last summer spent at the German Cancer Research Institute in Heidelberg, Germany. I'll be working in both the iGEM lab and a Medical Genetics research lab during summer 2009. My heritage is a mixture of English, Chinese, and Danish. iGEM helps move biology to a streamlined, standardized, abstracted process that opens the door to interdisciplinary collaborations and new applications. I'm excited about the many applications of Synthetic Biology and am enjoying the student-directed and team-oriented nature of iGEM.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<br />
<tr><td><div align="left"> <br />
<strong>Alina Ponomarev</strong><br><br />
<em>Biological Sciences</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/d/d6/UofAiGEM2009_Alina_IMG_5023.JPG" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I am currently a second year student in Biological Sciences and I am considering transferring into the Immunology and Infection program for my third year. I joined iGEM at the end of my first year and can honestly say that it has been an unbeatable learning experience. It is very exciting to think that the progress we have made can positively impact synthetic biology and society in general. In the future, my career goal is to become a Pediatrician. However, after doing labwork at the iGEM lab this summer, I have begun to consider a career in medical research. </div> <br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Kelly Robinson</strong><br><br />
<em>Hon. Biochemistry</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/9/92/UofA09_team_Kelly_Robinson.png" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I completed my BSc. in Biochemistry this year and have enrolled in the University of Alberta's Chemical Engineering program in hopes of fusing the fundamental science of biological machines with the entrepreneurial world of engineering. I got involved in iGEM a couple of years ago when I went to a presentation put on by one of the past teams. I ended up applying in the middle of the night on the last day of registration. That said, it has been a great experience ever since. I'm glad that this year I get the opportunity to do some full time work and really get into the project. Overall, iGEM has a lot to offer anyone who gets involved (including incorrigible advisors: you know who you are!).</div><br />
<br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr> <br />
<br />
<tr><td><div align="left"><br />
<strong>James Rodway</strong><br><br />
<em>Electrical Engineering</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/a/a5/UofA09_team_James_Rodway.jpg" ALIGN="LEFT" hspace="20"><br />
<br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I am currently finishing up a co-op Electrical Engineering degree, and am aiming to do some graduate work in more of the computer area, specifically modeling. At the University, I've been involved with the ARVP and iGEM.<br />
I haven't really had much time for hobbies in a while, but when I did I played more video games, the guitar, and I actually read books for entertainment value. During my time on last year's iGEM team I found that it was a pretty cool multidisciplinary project, which drew in a few different disciplines that I have never really interacted with previously. It was very impressive to see what we, and all the iGEM teams, had accomplished by the end of last year's competition.</div><br />
<br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Andy Spencer</strong><br><br />
<em>Honours Biochemistry</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/c/ca/UofA09_team_Andy_Spencer.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">In 5 years I see myself on one of two different paths, medicine or oncology research. I grew up in the Okanagan, British Columbia, and love spending my summers at the beach . In my spare time I enjoy playing squash and bouldering. iGEM to me represents a fundamental value of science as a discipline; that people can from diverse backgrounds with a common interest and curiosity can work as a cohesive team to overcome barriers and problems. </div><br />
<br />
</td></tr> <br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Jonathan Tam</strong><br><br />
<em>Honors Cell Biotechnology</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/e/e2/UofA09_team_Jonathan_Tam.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I have completed my Bachelor's degree in Honors Cell Biotechnology and intend to pursue a medical degree in Germany in the near future. I am very interested in the fields of Molecular Biology and Immunology. For the last two years, I have been keeping myself busy studying the development of macrophages in the lab of Dr. Daniel Barreda. Outside of the lab, I am an avid photographer and downhill mountain biker. The iGEM competition accelerates the development of Synthetic Biology as a field. To be a part of our University of Alberta team is an excellent opportunity for collaboration and advancement.</div><br />
</td></tr> <br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Jennifer Yau</strong><br><br />
<em>Honors Biochemistry</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/9/99/UofAiGEM2009_Jen_IMG_5048.JPG" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I am currently in the second year of my undergraduate program with the ultimate goal of attending graduate school to pursue a research career in Geriatric Medicine. Additional science related activities of mine include my interest in astronomy and promoting science to elementary students through volunteer work. Otherwise, I dedicate the majority of my free time to the arts of chainmaille, knitting, and jazz/classical piano. This is my first year on iGEM and I am ecstatic to be partaking in a project that requires such diverse disciplinary backgrounds. Not only will this be a great learning experience in so many perspectives, but also an opportunity to contribute significant ideas to the ongoing research in Synthetic Biology</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr> <br />
<br />
<tr><td><div align="left"><br />
<strong>Zach Wiltshire</strong> <br><br />
<em>BSc. Specialization in Cell Biology</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/0/0b/UofA09_team_Zach_Wiltshire2.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I am entering into my fourth year in the Cell Biology program at the U of A and have already experienced iGEM once before as a member of the 2008 National Institute for Nanotechnology team. Through my degree I have found myself to have interests ranging from immunology to both eukaryotic & prokaryotic cellular anatomy & physiology. I also happen to be very fond of molecules that fluoresce. My experience with iGEM last year opened my eyes to the possibilities which engineering biological systems can offer. Over the course of the 2009 competition I hope to have just as many opportunities to learn as I did last year.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<tr><td><br />
<a name="Team_Ad"></a><br />
<strong>The Supervisors</strong><br />
</tr><td><br />
<br />
<tr><td><br />
<strong>Mike Ellison</strong><br />
<img src="https://static.igem.org/mediawiki/2009/d/d5/UofA09_teamad_Mike_Ellison.jpg" ALIGN="LEFT" hspace="20"><br />
</td></tr><br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><br />
<br />
<strong>Doug Ridgway</strong><br />
<img src="https://static.igem.org/mediawiki/2009/c/cc/UofA09_teamad_Douglas_Ridgway.jpg" ALIGN="LEFT" hspace="20"></a><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><br />
<strong>James Maclagan</strong><br />
<img src="https://static.igem.org/mediawiki/2009/a/ab/UofAigem2009_JamesMIMG_4975.JPG" ALIGN="LEFT" hspace="20"></a><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</tr></td><br />
<tr><td><br />
<a name="Fac_Con"></a><br />
<strong>Faculty Consultants</strong><br />
</tr><td><br />
<br />
<tr><td><br />
<strong>Chris Backhouse</strong><br />
<br><br />
Department of Electrical Engineering<br />
<br><br />
christopher.backhouse@ualberta.ca<br />
<img src="http://www.ece.ualberta.ca/~chrisb/Graphics/Backhouse.jpg" ALIGN="LEFT" hspace="20"><br />
</td><br />
<br />
<td><br />
<div align="justify" ><br />
Nanobiotechnologies give us the ability to manipulate and sense at the level of individual molecules, with a tremendous potential impact on both human health and the economy. To a large extent, this potential is likely to be realised through the development of Lab on Chip (LOC) technologies. Although the LOC technologies are powerful, the complexity of the infrastructure required to support LOC operation has hindered the widespread adoption of LOC methods in life science applications. A central theme in the work of the Backhouse lab (<a href="http://www.ece.ualberta.ca/~aml/">the Applied Miniaturisation Lab, AML</a>) is the development of extremely inexpensive (e.g. $1000) systems for implementing nanobiotechnologies/molecular biology, especially for medical diagnostic applications.</div><br />
</td><br />
</tr><br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><br />
<br />
<strong>Robert Campbell</strong><br />
<br><br />
Department of Chemistry<br />
<br><br />
robert.e.campbell@ualberta.ca<br />
<img src="http://www.chem.ualberta.ca/faculty_staff/faculty/facultypics/campbell.jpg" width=200 height=274.19355 ALIGN="LEFT" hspace="20"></a><br />
</td><br />
<br />
<td><br />
<div align="justify" ><br />
Robert E. Campbell is an associate professor and Canada Research Chair in Bioanalytical Chemistry in the Department of Chemistry of the University of Alberta. Research in his laboratory, (<a href="http://www.chem.ualberta.ca/~campbell/">the Campbell Research Group</a>), is focused on protein engineering and the development of new fluorescent protein variants for construction of FRET-based biosensors.<br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><br />
<strong>Linda Reha-Krantz</strong><br />
<br><br />
Department of Biological Sciences<br />
<br><br />
Linda.Reha-Krantz@ualberta.ca<br />
<img src="http://www.biology.ualberta.ca/faculty/bio-187/uploads/images/reha_krantz.jpg" width=200 height=300 ALIGN="LEFT" hspace="20"></a><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</tr></td><br />
<br />
<tr><td><br />
<strong>Tracy Raivio</strong><br />
<br><br />
Department of Biological Sciences<br />
<br><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</tr></td><br />
<br />
<tr><td><br />
<strong>Jon Dennis</strong><br />
<br><br />
Department of Biological Sciences<br />
<br><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</tr></td><br />
<br />
<br />
<br />
<br />
</table><br />
<br />
</div><br />
<br />
</div></div><br />
<b class="b4f"></b><b class="b3f"></b><b class="b2f"></b><b class="b1f"></b><br />
</td><br />
</tr><br />
<br />
</table><br />
</html></div>
Wiltshir
http://2009.igem.org/File:UofA09_team_Zach_Wiltshire2.jpg
File:UofA09 team Zach Wiltshire2.jpg
2009-10-21T07:40:37Z
<p>Wiltshir: </p>
<hr />
<div></div>
Wiltshir
http://2009.igem.org/Team:Alberta/Team
Team:Alberta/Team
2009-10-21T07:38:19Z
<p>Wiltshir: </p>
<hr />
<div>{{:Team:Alberta/Template3}}<br />
<br />
<html><br />
<head><br />
<style type="text/css"><br />
.b1f, .b2f, .b3f, .b4f{font-size:1px; overflow:hidden; display:block;}<br />
.b1f {height:1px; background:#e1e1e1; margin:0 5px;}<br />
.b2f {height:1px; background:#e1e1e1; margin:0 3px;}<br />
.b3f {height:1px; background:#e1e1e1; margin:0 2px;}<br />
.b4f {height:2px; background:#e1e1e1; margin:0 1px;}<br />
.content {background: #e1e1e1;}<br />
.content div {margin-left: 5px;}<br />
</style><br />
</head><br />
<br />
<div class="all"><br />
<div style="background:#FFFFFF"><br />
<br />
<!-- adjust table width, main background and padding between cells and edge of background --><br />
<br />
<br />
<table width=60% style="background:#FFFFFF; padding:2px;"><br />
<br />
<tr><br />
<td style="height: 400; padding-left: 10px; padding-right: 10px; padding-top: 11px;"><br />
<b class="b1f"></b><b class="b2f"></b><b class="b3f"></b><b class="b4f"></b><br />
<div class="Recoli"><br />
<div style="height: 400; background:#FFFFFF; colorou line-height:100% padding: 3px 0px;"><br />
<h1>Project BioBytes</h1><br />
<br />
<!-- <div align="justify" style="padding-left:20px; padding-right:20px"> --><br />
<div align="justify"><br />
<br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/2009/8/8f/UofAiGEM2009_groupphoto_IMG_5009.JPG" width="320" height="407"><br />
</center><br />
<br />
<br> <br />
<br />
<table><br />
<br />
<tr><br />
<td><br />
<div align="left"><strong>Kalon Armstrong</strong> <br><br />
<em>Molecular Genetics</em> <br><br />
</div><br />
<td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/3/34/UofA09_team_Kalon_Armstrong.jpg" hspace="20"><br />
</td><br />
<td><br />
<div align="justify" >I've recently completed my BSc in Molecular Genetics and will be entering the Engineering program in the fall of 2009. Most of my growing-up took place in the small town of Cochrane, Alberta. My ultimate goals consist of working in the Biotechnology or health care industry. While my interest in music, movies, snowboarding, and hanging out with friends may seem stereotypical on the surface, they feel unique in their own right and keep me busy most of the time. This will be my first year on the U of A iGEM team and I am excited to help take the competition to a new level. I think that iGEM will be a great experience because it demands innovation and collaboration on levels rarely seen in undergraduate programs.</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td><br />
<tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Eric Bennett</strong><br><br />
<em>Electrical Biomedical Engineering</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/e/ef/UofA09_Eric_Bennett.JPG" hspace="20"><br />
</td><br />
<br />
<td><br />
<div align="justify" >I am entering my final year of engineering at the U of A. After graduation, I hope to do research either with a biotechnology company or in grad school. I think that iGEM is a great way to gain valuable experience and is an effective way of accelerating the field of synthetic biology. My interests include brain-machine interfacing, genetic engineering, and robotic control systems. My hobbies include playing guitar, video games, the occasional sport, reading, and fixing my car.</div><br />
</td><br />
</tr> <br />
<br />
<tr><br />
<td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Max Buchko</strong><br><br />
<em>Honors Biochemistry</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="http://igem.biochem.ualberta.ca/wiki/images/3/3d/UofA_iGEM09_Max_Pic1.png" ALIGN="LEFT" hspace="20"><br />
</td><br />
<br />
<td><br />
<div align="justify" style="padding-right:20px">I am in my third year of Honors Biochemistry and wish to pursue a career in medicine. In my time away from the lab I enjoy a wide variety of sports including soccer, boxing, and rifle silhouette shooting. I have also been known to strum a chord or two at an intolerable volume to the annoyance of the people living upstairs.<br />
I hope for the best with iGEM at MIT in 2009. This competition is a means of proving yourself at an exemplary level amongst many of the top international minds, and it is this challenge that I look forward to the most.</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Oscar Cortés</strong><br><br />
<em>Bsc. Specialization molecular genetics</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/7/77/Oscar.jpg" ALIGN="LEFT" hspace="20"><br />
</td><br />
<td><br />
<div align="justify" style="padding-right:20px">My future plans include entering into a Masters program in Medical Genetics or Human Genetics, and pursuing this discipline towards a PhD. I enjoy reading books about the human genome and advancements in stem cell research. In my spare time I like to play a variety of sports that I am not necessarily good at: soccer, softball, and dodge ball. I see iGEM as an extraordinary opportunity for me to be exposed to real life research, in which my knowledge of molecular genetics will be challenged, and will help me further my understanding of Synthetic Biology.<br />
"Man with all his noble qualities still bears in his bodily frame the indelible stamp of his lowly origin"- Charles Darwin</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td><br />
</tr> <br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Anh Dao</strong><br><br />
<em>Biological Sciences and Chemistry</em><br> <br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/c/cf/UofA09_team_Ahn_Dao.jpg" ALIGN="LEFT" hspace="20"><br />
</td><br />
<br />
<td><br />
<div align="justify" style="padding-right:20px">I am interested in a research career after I finish my degree. I am leaning towards the field of Microbiology to study the many micro-organisms that have not yet been discovered. However, I may enroll in graduate studies after my undergraduate degree to expand my knowledge and gain more experience in the laboratory. Being in a competitive team and atmosphere is a motivating and exciting opportunity that I do not want to miss out on. By creating the smallest artificial E. coli genome we can extend future research. We are attempting to understand and standardize the E. coli genome so that this methodology can be applied to more complex model organisms.</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Uchechukwu Davidson</strong><br><br />
<em>Honors biochemistry</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/d/d5/Uche.jpg" ALIGN="LEFT" hspace="20"><br />
</td><br />
<td><br />
<div align="justify" style="padding-right:20px">I am in my third year of Biochemistry at the University of Alberta. I wish to pursue a career in medicine after my degree. I enjoy sports, movies, and music at my time away from my studies. The iGEM provides an opportunity to experience creativity, innovation, and ingenuity which are sometimes absent at the undergraduate level of science.</div><br />
</td><br />
</tr><br />
<br />
<tr> <br />
<td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Youness Elkhalidy</strong><br><br />
<em>Honors immunology and infection</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/f/fe/UofAiGEM2009_Youness_IMG_5049.JPG" width="200" height="300" ALIGN="LEFT" hspace="20"><br />
</td><br />
<td><br />
I am a first year student at the University of Alberta. I hope to enter medical school in the near future. I am currently taking part in mitotic-spindle regulation research from a genetics perspective. I have a passion for science and enjoy sports such as basketball and soccer. iGEM is an great learning experience not only in the cutting-edge field of Synthetic Biology but also in leadership and business management. I will enjoy taking part in research that combines many fields of science as well as socialize with many of my team members who share common interests.<br />
</td><br />
</tr><br />
<br />
<tr><br />
<td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Justin Fedor</strong><br><br />
<em>Honours Biochemistry</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2009/0/0d/IMG_4985.JPG" ALIGN="LEFT" hspace="20"><br />
</td><br />
<br />
<td><br />
<div align="justify" style="padding-right:20px">I have completed my undergraduate degree in Biochemistry this year and have began my PhD studies this September. I play piano and am attempting to learn the cello. My nerdy tendencies are obvious when I say that I like Star Trek TNG but not DS9. Last summer I worked in the biomedical research lab of Dr. Larry Unsworth of the National Institute for Nanotechnology (NINT), which has further piqued my interest in the field of nanotechnology. In the hopefully not too distant future I plan on becoming a researcher studying the mechanisms of membrane bound enzymes, particularly oxidoreductases. iGEM has proved a fantastic experience, it has served to mature me further as a scientist.</div><br />
</td><br />
</tr><br />
<br />
<tr><td colspan = "2"> <br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><br />
<td><br />
<div align="left"><br />
<strong>Jason Gardiner</strong> <br><br />
<em>BSc. Specialization in Botany</em><br><br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/2/2b/UofA09_team_Jason_Gardiner.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">Jason is a veteran of iGEM 2007, where his team "The Butanerds" won first place in the Energy track. His hobbies include Softball, Beach volleyball, soccer, as well as playing the guitar and fiddling with the iGEM 2009 Wiki. Jason has applied to continue his education in graduate studies at the University of Alberta in 2010. His degree in Botany focuses mostly on the molecular side of plants and he hopes to use this knowledge applying Synthetic Biology to plants. One day he hopes to solve all of the world's problems using plants and Synthetic Biology.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><br />
<div align="left"><br />
<strong>Erin Garside</strong> <br><br />
<em>Biological Sciences</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/6/60/UofA09_team_Erin_Garside.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I completed my BSc. degree this year and plan to do graduate work in biochemistry. After that - who knows? I think iGEM will be a great experience, and that it's about time Synthetic Biology really took off. In my spare time I like to read, play computer games (especially the Sims) and raise cats.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Boris Henriquez</strong><br><br />
<em>Biological Sciences</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/e/ec/UofA09_team_Boris_Heneriquez.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I have just completed the third year of my BSc. degree. My favorite classes so far have been in the fields of Biochemistry and Genetics. I am most interested in human development and embryogenesis and hope to learn more about these topics in my fourth year. My ultimate goal is to have a career in the medical industry as both a physician and as a researcher. When I'm not busy with school or work my usual activities are pretty standard. I like to hang out with friends and family and I crave the outdoors. This is my first year with iGEM and I'm really excited to learn more about Synthetic Biology. </div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Stephen Jahns</strong><br><br />
<em>Molecular Genetics</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/a/a8/Alberta_steve.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I am in my second year at the University of Alberta. After taking a year of engineering, I decided to transfer to molecular genetics in order to fulfill my childhood desire of creating an army of giant bat-rabbits. I plan on going on through to graduate school to earn a PhD in order to reach my goals. In my spare time I like to run around, ride my bike, play music, cook delicious food, and pretty much all the other fun things kids are doing these days. In my first year I helped to build a car with the U of A's Formula SAE fabrication team. I had a good time building stuff out of metal, and this year I know that I'm going to have a blast building (or at least attempting to build) a novel organism out of DNA parts.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<br />
<tr><td><div align="left"><br />
<strong>Eric Leung</strong> <br><br />
<em>Honors Pharmacology</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/c/c7/Alberta_Eric_Leung.JPG" ALIGN="LEFT" hspace="20"><br />
<br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I am currently in my final year of an Honors Pharmacology degree. What I do after this degree is up in the air but it is definitely something in the health sciences. In my spare time, I like to play basketball, badminton, run, and catch up on TV shows. Soon I hope I can become a professional baker in my free time. Traveling to Europe, Mexico, and Japan is also in the books somewhere along the way.</div><br />
<br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<br />
<tr><td><div align="left"><br />
<strong>David Lloyd</strong><br> <br />
<em> Biochemistry</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/6/6f/UofA09_team_David_Lloyd.jpg" ALIGN="LEFT" hspace="20"><br />
<br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I am a fourth year student, studying at the University of Alberta. While Biochemistry is my major, I have many interests including Genetics, Immunology, Cell Biology, Bioinformatics, and Microbiology. In my spare time I can be found playing sports like soccer, volleyball, or squash, as well as playing piano, listening to music, and playing video games. In the future, I hope to continue into a graduate program in Biochemistry, Cell Biology, Synthetic Biology, or in another field. iGEM is of great interest to myself because of its application to the future of Synthetic Biology. It is an awesome challenge which will hopefully help me to cultivate myself.</div><br />
<br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Enoch Ng</strong><br><br />
<em>Biological Sciences/Business</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/7/7c/UofA09_team_Enoch_Ng.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I am entering my fourth year of studies at the University of Alberta, and am looking forward to the challenge that iGEM provides. In the future I hope to travel across the world and meet people ranging from Meshaal to Obama and remain a glorified generalist. </div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Emera Nguyen</strong><br><br />
<em>BSc. Biological Sciences/Economics</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/5/52/UofA09_team_Emera_Nguyen.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I am a BSc student entering my fourth year in a Biological Sciences major and Economics minor. This year will be my second year on an iGEM team, and my first year representing the U of A team. In my downtime, things I enjoy include spending quality time with friends and family and traveling to lesser-known parts of the world. One year's experience later, the iGEM competition still impresses me with the unique opportunities it provides for student growth. This research competition is one of the highlights of my undergraduate experience.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr> <br />
<br />
<br />
<tr><td><div align="left"><br />
<strong>Mitch Paquette</strong><br><br />
<em>BSc. Honors Molecular Genetics</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/1/12/IMG_5052.jpg" width="200" height="300" ALIGN="LEFT" hspace="20"><br />
<br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I have transfered into the honors program in Molecular Genetics from a BSc General program in Physical Sciences. After my degree is completed I plan to apply to graduate studies in Molecular Biology, after which I hope to work in research or academia. What excites me most about iGEM is the opportunity to gain research experience in such a new field.</div><br />
</td></tr> <br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Amber Paul</strong><br><br />
<em>BSc. Specialization Immunology and Infection</em><br><br />
</div></td></tr><br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/5/5f/IMG_4987.jpg" width="200" height="300" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I plan to find the cure for cancer. Seriously. Will be attending graduate school to earn a PhD following my undergraduate degree. I would like to live somewhere warm that allows me to do research, preferably Maui. iGEM is a developmental forefront to Synthetic Biology, something that I love to be part of. Its challenging and fun. To be able to determine the minimal genes for a simple prokaryote, we provide a scaffold to future research on larger, more complex cells such as cancerous tissues, simple eukaryotes, etc.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Julia Pon</strong><br><br />
<em>Honors Molecular Genetics</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/e/ec/UofA_Julia_Pon_photo.JPG" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">My future plans include pursuing PhD in Medical Genetics. I've worked in biological research labs for the past two summers, with last summer spent at the German Cancer Research Institute in Heidelberg, Germany. I'll be working in both the iGEM lab and a Medical Genetics research lab during summer 2009. My heritage is a mixture of English, Chinese, and Danish. iGEM helps move biology to a streamlined, standardized, abstracted process that opens the door to interdisciplinary collaborations and new applications. I'm excited about the many applications of Synthetic Biology and am enjoying the student-directed and team-oriented nature of iGEM.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<br />
<tr><td><div align="left"> <br />
<strong>Alina Ponomarev</strong><br><br />
<em>Biological Sciences</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/d/d6/UofAiGEM2009_Alina_IMG_5023.JPG" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I am currently a second year student in Biological Sciences and I am considering transferring into the Immunology and Infection program for my third year. I joined iGEM at the end of my first year and can honestly say that it has been an unbeatable learning experience. It is very exciting to think that the progress we have made can positively impact synthetic biology and society in general. In the future, my career goal is to become a Pediatrician. However, after doing labwork at the iGEM lab this summer, I have begun to consider a career in medical research. </div> <br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Kelly Robinson</strong><br><br />
<em>Hon. Biochemistry</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/9/92/UofA09_team_Kelly_Robinson.png" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I completed my BSc. in Biochemistry this year and have enrolled in the University of Alberta's Chemical Engineering program in hopes of fusing the fundamental science of biological machines with the entrepreneurial world of engineering. I got involved in iGEM a couple of years ago when I went to a presentation put on by one of the past teams. I ended up applying in the middle of the night on the last day of registration. That said, it has been a great experience ever since. I'm glad that this year I get the opportunity to do some full time work and really get into the project. Overall, iGEM has a lot to offer anyone who gets involved (including incorrigible advisors: you know who you are!).</div><br />
<br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr> <br />
<br />
<tr><td><div align="left"><br />
<strong>James Rodway</strong><br><br />
<em>Electrical Engineering</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/a/a5/UofA09_team_James_Rodway.jpg" ALIGN="LEFT" hspace="20"><br />
<br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I am currently finishing up a co-op Electrical Engineering degree, and am aiming to do some graduate work in more of the computer area, specifically modeling. At the University, I've been involved with the ARVP and iGEM.<br />
I haven't really had much time for hobbies in a while, but when I did I played more video games, the guitar, and I actually read books for entertainment value. During my time on last year's iGEM team I found that it was a pretty cool multidisciplinary project, which drew in a few different disciplines that I have never really interacted with previously. It was very impressive to see what we, and all the iGEM teams, had accomplished by the end of last year's competition.</div><br />
<br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Andy Spencer</strong><br><br />
<em>Honours Biochemistry</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/c/ca/UofA09_team_Andy_Spencer.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">In 5 years I see myself on one of two different paths, medicine or oncology research. I grew up in the Okanagan, British Columbia, and love spending my summers at the beach . In my spare time I enjoy playing squash and bouldering. iGEM to me represents a fundamental value of science as a discipline; that people can from diverse backgrounds with a common interest and curiosity can work as a cohesive team to overcome barriers and problems. </div><br />
<br />
</td></tr> <br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Jonathan Tam</strong><br><br />
<em>Honors Cell Biotechnology</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/e/e2/UofA09_team_Jonathan_Tam.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I have completed my Bachelor's degree in Honors Cell Biotechnology and intend to pursue a medical degree in Germany in the near future. I am very interested in the fields of Molecular Biology and Immunology. For the last two years, I have been keeping myself busy studying the development of macrophages in the lab of Dr. Daniel Barreda. Outside of the lab, I am an avid photographer and downhill mountain biker. The iGEM competition accelerates the development of Synthetic Biology as a field. To be a part of our University of Alberta team is an excellent opportunity for collaboration and advancement.</div><br />
</td></tr> <br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><div align="left"><br />
<strong>Jennifer Yau</strong><br><br />
<em>Honors Biochemistry</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/9/99/UofAiGEM2009_Jen_IMG_5048.JPG" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<div align="justify" style="padding-right:20px">I am currently in the second year of my undergraduate program with the ultimate goal of attending graduate school to pursue a research career in Geriatric Medicine. Additional science related activities of mine include my interest in astronomy and promoting science to elementary students through volunteer work. Otherwise, I dedicate the majority of my free time to the arts of chainmaille, knitting, and jazz/classical piano. This is my first year on iGEM and I am ecstatic to be partaking in a project that requires such diverse disciplinary backgrounds. Not only will this be a great learning experience in so many perspectives, but also an opportunity to contribute significant ideas to the ongoing research in Synthetic Biology</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr> <br />
<br />
<tr><td><div align="left"><br />
<strong>Zach Wiltshire</strong> <br><br />
<em>BSc. Specialization in Cell Biology</em><br><br />
</div></td></tr><br />
<br />
<tr><td><br />
<img src="https://static.igem.org/mediawiki/2009/1/19/UofA09_team_Zach_Wiltshire2.jpg" ALIGN="LEFT" hspace="20"><br />
</td><td><br />
<br />
<div align="justify" style="padding-right:20px">I am entering into my fourth year in the Cell Biology program at the U of A and have already experienced iGEM once before as a member of the 2008 National Institute for Nanotechnology team. Through my degree I have found myself to have interests ranging from immunology to both eukaryotic & prokaryotic cellular anatomy & physiology. I also happen to be very fond of molecules that fluoresce. My experience with iGEM last year opened my eyes to the possibilities which engineering biological systems can offer. Over the course of the 2009 competition I hope to have just as many opportunities to learn as I did last year.</div><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<tr><td><br />
<a name="Team_Ad"></a><br />
<strong>The Supervisors</strong><br />
</tr><td><br />
<br />
<tr><td><br />
<strong>Mike Ellison</strong><br />
<img src="https://static.igem.org/mediawiki/2009/d/d5/UofA09_teamad_Mike_Ellison.jpg" ALIGN="LEFT" hspace="20"><br />
</td></tr><br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><br />
<br />
<strong>Doug Ridgway</strong><br />
<img src="https://static.igem.org/mediawiki/2009/c/cc/UofA09_teamad_Douglas_Ridgway.jpg" ALIGN="LEFT" hspace="20"></a><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><br />
<strong>James Maclagan</strong><br />
<img src="https://static.igem.org/mediawiki/2009/a/ab/UofAigem2009_JamesMIMG_4975.JPG" ALIGN="LEFT" hspace="20"></a><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</tr></td><br />
<tr><td><br />
<a name="Fac_Con"></a><br />
<strong>Faculty Consultants</strong><br />
</tr><td><br />
<br />
<tr><td><br />
<strong>Chris Backhouse</strong><br />
<br><br />
Department of Electrical Engineering<br />
<br><br />
christopher.backhouse@ualberta.ca<br />
<img src="http://www.ece.ualberta.ca/~chrisb/Graphics/Backhouse.jpg" ALIGN="LEFT" hspace="20"><br />
</td><br />
<br />
<td><br />
<div align="justify" ><br />
Nanobiotechnologies give us the ability to manipulate and sense at the level of individual molecules, with a tremendous potential impact on both human health and the economy. To a large extent, this potential is likely to be realised through the development of Lab on Chip (LOC) technologies. Although the LOC technologies are powerful, the complexity of the infrastructure required to support LOC operation has hindered the widespread adoption of LOC methods in life science applications. A central theme in the work of the Backhouse lab (<a href="http://www.ece.ualberta.ca/~aml/">the Applied Miniaturisation Lab, AML</a>) is the development of extremely inexpensive (e.g. $1000) systems for implementing nanobiotechnologies/molecular biology, especially for medical diagnostic applications.</div><br />
</td><br />
</tr><br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><br />
<br />
<strong>Robert Campbell</strong><br />
<br><br />
Department of Chemistry<br />
<br><br />
robert.e.campbell@ualberta.ca<br />
<img src="http://www.chem.ualberta.ca/faculty_staff/faculty/facultypics/campbell.jpg" width=200 height=274.19355 ALIGN="LEFT" hspace="20"></a><br />
</td><br />
<br />
<td><br />
<div align="justify" ><br />
Robert E. Campbell is an associate professor and Canada Research Chair in Bioanalytical Chemistry in the Department of Chemistry of the University of Alberta. Research in his laboratory, (<a href="http://www.chem.ualberta.ca/~campbell/">the Campbell Research Group</a>), is focused on protein engineering and the development of new fluorescent protein variants for construction of FRET-based biosensors.<br />
</div><br />
</td><br />
</tr><br />
<br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</td></tr><br />
<br />
<tr><td><br />
<strong>Linda Reha-Krantz</strong><br />
<br><br />
Department of Biological Sciences<br />
<br><br />
Linda.Reha-Krantz@ualberta.ca<br />
<img src="http://www.biology.ualberta.ca/faculty/bio-187/uploads/images/reha_krantz.jpg" width=200 height=300 ALIGN="LEFT" hspace="20"></a><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</tr></td><br />
<br />
<tr><td><br />
<strong>Tracy Raivio</strong><br />
<br><br />
Department of Biological Sciences<br />
<br><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</tr></td><br />
<br />
<tr><td><br />
<strong>Jon Dennis</strong><br />
<br><br />
Department of Biological Sciences<br />
<br><br />
</td></tr><br />
<br />
<tr><td colspan = "2"><br />
<br><br />
<hr style=width:66% align=left><br />
</tr></td><br />
<br />
<br />
<br />
<br />
</table><br />
<br />
</div><br />
<br />
</div></div><br />
<b class="b4f"></b><b class="b3f"></b><b class="b2f"></b><b class="b1f"></b><br />
</td><br />
</tr><br />
<br />
</table><br />
</html></div>
Wiltshir
http://2009.igem.org/Team:Alberta/Template3
Team:Alberta/Template3
2009-10-21T00:10:42Z
<p>Wiltshir: </p>
<hr />
<div><html><br />
<title>University of Alberta - BioBytes</title><br />
<br />
<link rel="stylesheet" type="text/css" href="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/main.css&action=raw&ctype=text/css" /><br />
<link rel="stylesheet" type="text/css" href="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/dd.css&action=raw&ctype=text/css" /><br />
<!--[if IE]><br />
<style type="text/css"><br />
#utilNav ul li {list-style-image: none;}<br />
#suppNav ul li {list-style-image: none;}<br />
#mainNav ul li {list-style-image: none;}<br />
</style><br />
<![endif]--><br />
<script type="text/javascript" src="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/java.js&action=raw&ctype=text/javascript"></script><br />
<script type="text/javascript"><br />
function ddnav() {<br />
$('#ddNav li').hover(<br />
function () {<br />
$(this).find('div:first').css('display','block');},<br />
function () {<br />
$(this).find('div:first').css('display','none');}<br />
);<br />
}<br />
function ddnavsub() {<br />
$('#ddNav span > a').toggle(<br />
function () {<br />
$(this).removeClass("expand").addClass("collapse");<br />
$(this).parent().find('div:first').slideDown('fast');<br />
$(this).hover(<br />
function (){$(this).css('background-color','#e5e5e0')},<br />
function (){$(this).css('background-color','#e5e5e0')});},<br />
function () {<br />
$(this).removeClass("collapse").addClass("expand");<br />
$(this).parent().find('div:first').css('display','none');<br />
$(this).hover(<br />
function (){$(this).css('background-color','#F5F5F0')},<br />
function (){$(this).css('background-color','#FFFFFF')});}<br />
).addClass('expand');<br />
}<br />
<br />
function ddmozilla() {<br />
ddtoggle();<br />
}<br />
<br />
$(function () {<br />
ddnav();<br />
ddnavsub();<br />
<br />
if($.browser.mozilla) {ddmozilla();}<br />
});<br />
</script><br />
<center><br />
<div id="header"><br />
<img src="https://static.igem.org/mediawiki/2009/0/04/UofA_biobytes_HeaderBanner5.png" width="965" height="235"><br />
<br />
<!-- logo --><br />
<div id="logo"><br />
</div><br />
<!-- END logo --><br />
<br />
<!-- main navigation --><br />
<div id="mainNav"><br />
<ul id="ddNav"><br />
<br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta">Home</a><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/Team">Team</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Team">Undergraduates</a><br />
<a href="https://2009.igem.org/Team:Alberta/Team#Team_Ad">Team Advisors</a><br />
<a href="https://2009.igem.org/Team:Alberta/Team#Fac_Con">Faculty Consultants</a><br />
</div><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta">Project</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Bioinformatics">Bioinformatics</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/assemblyoverview">Assembly Process</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Recombineering">Recombineering</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Outreach">Outreach</a><br />
</div><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/MedalRequirements">Achievements</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/MedalRequirements">Medals & Prizes</a><br />
<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Alberta" target="_blank">Parts Registry</a><br />
<br />
</div><br />
</li><br />
<li><br />
<a>Lab</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Calendar">Lab Notebook</a><br />
<a href="https://2009.igem.org/Team:Alberta/Blackboard">Lab Blackboard</a><br />
<a href="https://2009.igem.org/Team:Alberta/Protocols">Protocols</a><br />
<br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/References/Keyword">References</a><br />
</li><br />
<li><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
<!-- END main navigation --><br />
<br />
</div><br />
</center><br />
</html><br />
<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br />
{{:Team:Alberta/LeftBar}}<br />
{{:Team:Alberta/RightBar}}<br />
<html><br />
<style type="text/css"><br />
body {background-color:#f8f8f8;}<br />
</style><br />
</html></div>
Wiltshir
http://2009.igem.org/Team:Alberta/TemplateSc
Team:Alberta/TemplateSc
2009-10-21T00:10:24Z
<p>Wiltshir: </p>
<hr />
<div><html><br />
<title>University of Alberta - BioBytes</title><br />
<br />
<link rel="stylesheet" type="text/css" href="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/main.css&action=raw&ctype=text/css" /><br />
<link rel="stylesheet" type="text/css" href="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/dd.css&action=raw&ctype=text/css" /><br />
<!--[if IE]><br />
<style type="text/css"><br />
#utilNav ul li {list-style-image: none;}<br />
#suppNav ul li {list-style-image: none;}<br />
#mainNav ul li {list-style-image: none;}<br />
</style><br />
<![endif]--><br />
<script type="text/javascript" src="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/java.js&action=raw&ctype=text/javascript"></script><br />
<script type="text/javascript"><br />
function ddnav() {<br />
$('#ddNav li').hover(<br />
function () {<br />
$(this).find('div:first').css('display','block');},<br />
function () {<br />
$(this).find('div:first').css('display','none');}<br />
);<br />
}<br />
function ddnavsub() {<br />
$('#ddNav span > a').toggle(<br />
function () {<br />
$(this).removeClass("expand").addClass("collapse");<br />
$(this).parent().find('div:first').slideDown('fast');<br />
$(this).hover(<br />
function (){$(this).css('background-color','#e5e5e0')},<br />
function (){$(this).css('background-color','#e5e5e0')});},<br />
function () {<br />
$(this).removeClass("collapse").addClass("expand");<br />
$(this).parent().find('div:first').css('display','none');<br />
$(this).hover(<br />
function (){$(this).css('background-color','#F5F5F0')},<br />
function (){$(this).css('background-color','#FFFFFF')});}<br />
).addClass('expand');<br />
}<br />
<br />
function ddmozilla() {<br />
ddtoggle();<br />
}<br />
<br />
$(function () {<br />
ddnav();<br />
ddnavsub();<br />
<br />
if($.browser.mozilla) {ddmozilla();}<br />
});<br />
</script><br />
<center><br />
<div id="header"><br />
<img src="https://static.igem.org/mediawiki/2009/0/04/UofA_biobytes_HeaderBanner5.png" width="965" height="235"><br />
<br />
<!-- logo --><br />
<div id="logo"><br />
</div><br />
<!-- END logo --><br />
<br />
<!-- main navigation --><br />
<div id="mainNav"><br />
<ul id="ddNav"><br />
<br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta">Home</a><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/Team">Team</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Team">Undergraduates</a><br />
<a href="https://2009.igem.org/Team:Alberta/Team#Team_Ad">Team Advisors</a><br />
<a href="https://2009.igem.org/Team:Alberta/Team#Fac_Con">Faculty Consultants</a><br />
</div><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta">Project</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Bioinformatics">Bioinformatics</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/assemblyoverview">Assembly Process</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Recombineering">Recombineering</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Outreach">Outreach</a><br />
</div><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/MedalRequirements">Achievements</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/MedalRequirements">Medals & Prizes</a><br />
<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Alberta" target="_blank">Parts Registry</a><br />
<br />
</div><br />
</li><br />
<li><br />
<a>Lab</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Calendar">Lab Notebook</a><br />
<a href="https://2009.igem.org/Team:Alberta/Blackboard">Lab Blackboard</a><br />
<a href="https://2009.igem.org/Team:Alberta/Protocols">Protocols</a><br />
<br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/References/Keyword">References</a><br />
</li><br />
<li><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
<!-- END main navigation --><br />
<br />
</div><br />
</center><br />
</html><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br />
{{:Team:Alberta/LeftBar}}<br />
<br />
<html><br />
<style type="text/css"><br />
body {background-color:#f8f8f8;}<br />
</style><br />
</html></div>
Wiltshir
http://2009.igem.org/User:Wiltshir
User:Wiltshir
2009-10-20T18:05:11Z
<p>Wiltshir: New page: <br> ===Zach Wiltshire=== University of Alberta & National Institute for Nanotechnology zach [dot] wiltshire [at] ualberta [dot] ca [[https://2009.igem.org/Team:Alberta<nowiki>2009 ...</p>
<hr />
<div><br><br />
<br />
===Zach Wiltshire===<br />
<br />
University of Alberta &<br />
National Institute for Nanotechnology<br />
<br />
zach [dot] wiltshire [at] ualberta [dot] ca<br />
<br />
[[https://2009.igem.org/Team:Alberta<nowiki>2009 iGEM Project </nowiki>]] - Project BioBytes<br />
<br><br />
[[https://2008.igem.org/Team:Alberta_NINT<nowiki>2008 iGEM Project </nowiki>]] - Project Logi-coli<br />
<br />
<br><br></div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/On-chip_oligonucleotide_ligation_assay_using_one-dimensional_micro%EF%AC%82uidic_beads_array_for_the_detection_of_low-abundant_DNA_point_mutations
Team:Alberta/References/Publications/On-chip oligonucleotide ligation assay using one-dimensional microfluidic beads array for the detection of low-abundant DNA point mutations
2009-10-20T08:08:57Z
<p>Wiltshir: New page: Authors Journal #### MONTH;##(#):PAGE-PAGE <b>Abstract:</b> ---- '''Link:''' [http://eeeeeeeeeeeee NAME]</p>
<hr />
<div>Authors<br />
<br />
Journal #### MONTH;##(#):PAGE-PAGE<br />
<br />
<br />
<b>Abstract:</b> ----<br />
<br />
<br />
<br />
'''Link:''' [http://eeeeeeeeeeeee NAME]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Nucleic_acid_purification_using_microfabricated_silicon_structures
Team:Alberta/References/Publications/Nucleic acid purification using microfabricated silicon structures
2009-10-20T08:08:29Z
<p>Wiltshir: New page: Authors Journal #### MONTH;##(#):PAGE-PAGE <b>Abstract:</b> ---- '''Link:''' [http://eeeeeeeeeeeee NAME]</p>
<hr />
<div>Authors<br />
<br />
Journal #### MONTH;##(#):PAGE-PAGE<br />
<br />
<br />
<b>Abstract:</b> ----<br />
<br />
<br />
<br />
'''Link:''' [http://eeeeeeeeeeeee NAME]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Microfabricated_Device_for_DNA_and_RNA_Amplification_by_Continuous-Flow_Polymerase_Chain_Reaction_and_Reverse_Transcription-Polymerase_Chain_Reaction_with_Cycle_Number_Selection
Team:Alberta/References/Publications/Microfabricated Device for DNA and RNA Amplification by Continuous-Flow Polymerase Chain Reaction and Reverse Transcription-Polymerase Chain Reaction with Cycle Number Selection
2009-10-20T08:08:04Z
<p>Wiltshir: New page: Authors Journal #### MONTH;##(#):PAGE-PAGE <b>Abstract:</b> ---- '''Link:''' [http://eeeeeeeeeeeee NAME]</p>
<hr />
<div>Authors<br />
<br />
Journal #### MONTH;##(#):PAGE-PAGE<br />
<br />
<br />
<b>Abstract:</b> ----<br />
<br />
<br />
<br />
'''Link:''' [http://eeeeeeeeeeeee NAME]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Microelectromagnet_for_magnetic_manipulation_in_lab-on-a-chip_systems
Team:Alberta/References/Publications/Microelectromagnet for magnetic manipulation in lab-on-a-chip systems
2009-10-20T08:07:53Z
<p>Wiltshir: New page: Authors Journal #### MONTH;##(#):PAGE-PAGE <b>Abstract:</b> ---- '''Link:''' [http://eeeeeeeeeeeee NAME]</p>
<hr />
<div>Authors<br />
<br />
Journal #### MONTH;##(#):PAGE-PAGE<br />
<br />
<br />
<b>Abstract:</b> ----<br />
<br />
<br />
<br />
'''Link:''' [http://eeeeeeeeeeeee NAME]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Magnetic_bead_handling_on-chip:_new_opportunities_for_analytical_applications
Team:Alberta/References/Publications/Magnetic bead handling on-chip: new opportunities for analytical applications
2009-10-20T08:07:44Z
<p>Wiltshir: New page: Authors Journal #### MONTH;##(#):PAGE-PAGE <b>Abstract:</b> ---- '''Link:''' [http://eeeeeeeeeeeee NAME]</p>
<hr />
<div>Authors<br />
<br />
Journal #### MONTH;##(#):PAGE-PAGE<br />
<br />
<br />
<b>Abstract:</b> ----<br />
<br />
<br />
<br />
'''Link:''' [http://eeeeeeeeeeeee NAME]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Development_of_a_microfluidic_biosensor_module_for_pathogen_detection
Team:Alberta/References/Publications/Development of a microfluidic biosensor module for pathogen detection
2009-10-20T08:07:36Z
<p>Wiltshir: New page: Authors Journal #### MONTH;##(#):PAGE-PAGE <b>Abstract:</b> ---- '''Link:''' [http://eeeeeeeeeeeee NAME]</p>
<hr />
<div>Authors<br />
<br />
Journal #### MONTH;##(#):PAGE-PAGE<br />
<br />
<br />
<b>Abstract:</b> ----<br />
<br />
<br />
<br />
'''Link:''' [http://eeeeeeeeeeeee NAME]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/An_integrated_microfluidic_biochemical_detection_system_forprotein_analysis_with_magnetic_bead-based_samplingcapabilities
Team:Alberta/References/Publications/An integrated microfluidic biochemical detection system forprotein analysis with magnetic bead-based samplingcapabilities
2009-10-20T08:07:28Z
<p>Wiltshir: New page: Authors Journal #### MONTH;##(#):PAGE-PAGE <b>Abstract:</b> ---- '''Link:''' [http://eeeeeeeeeeeee NAME]</p>
<hr />
<div>Authors<br />
<br />
Journal #### MONTH;##(#):PAGE-PAGE<br />
<br />
<br />
<b>Abstract:</b> ----<br />
<br />
<br />
<br />
'''Link:''' [http://eeeeeeeeeeeee NAME]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/TemplateSc
Team:Alberta/TemplateSc
2009-10-20T08:06:18Z
<p>Wiltshir: </p>
<hr />
<div><html><br />
<link rel="stylesheet" type="text/css" href="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/main.css&action=raw&ctype=text/css" /><br />
<link rel="stylesheet" type="text/css" href="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/dd.css&action=raw&ctype=text/css" /><br />
<!--[if IE]><br />
<style type="text/css"><br />
#utilNav ul li {list-style-image: none;}<br />
#suppNav ul li {list-style-image: none;}<br />
#mainNav ul li {list-style-image: none;}<br />
</style><br />
<![endif]--><br />
<script type="text/javascript" src="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/java.js&action=raw&ctype=text/javascript"></script><br />
<script type="text/javascript"><br />
function ddnav() {<br />
$('#ddNav li').hover(<br />
function () {<br />
$(this).find('div:first').css('display','block');},<br />
function () {<br />
$(this).find('div:first').css('display','none');}<br />
);<br />
}<br />
function ddnavsub() {<br />
$('#ddNav span > a').toggle(<br />
function () {<br />
$(this).removeClass("expand").addClass("collapse");<br />
$(this).parent().find('div:first').slideDown('fast');<br />
$(this).hover(<br />
function (){$(this).css('background-color','#e5e5e0')},<br />
function (){$(this).css('background-color','#e5e5e0')});},<br />
function () {<br />
$(this).removeClass("collapse").addClass("expand");<br />
$(this).parent().find('div:first').css('display','none');<br />
$(this).hover(<br />
function (){$(this).css('background-color','#F5F5F0')},<br />
function (){$(this).css('background-color','#FFFFFF')});}<br />
).addClass('expand');<br />
}<br />
<br />
function ddmozilla() {<br />
ddtoggle();<br />
}<br />
<br />
$(function () {<br />
ddnav();<br />
ddnavsub();<br />
<br />
if($.browser.mozilla) {ddmozilla();}<br />
});<br />
</script><br />
<center><br />
<div id="header"><br />
<img src="https://static.igem.org/mediawiki/2009/0/04/UofA_biobytes_HeaderBanner5.png" width="965" height="235"><br />
<br />
<!-- logo --><br />
<div id="logo"><br />
</div><br />
<!-- END logo --><br />
<br />
<!-- main navigation --><br />
<div id="mainNav"><br />
<ul id="ddNav"><br />
<br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta">Home</a><br />
</li><br />
<li><br />
<a>Team</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Team">Undergraduates</a><br />
<a href="https://2009.igem.org/Team:Alberta/Team#Team_Ad">Team Advisors</a><br />
<a href="https://2009.igem.org/Team:Alberta/Team#Fac_Con">Faculty Consultants</a><br />
</div><br />
</li><br />
<li><br />
<a>Project</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Bioinformatics">Bioinformatics</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/assemblyoverview">DNA Assembly</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Chromosome_Assembly">Chromosome Assembly</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Ethics_Debate/Overview">Ethics Debate</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Outreach">Outreach</a><br />
</div><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/MedalRequirements">Deliverables</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/MedalRequirements">Deliverables</a><br />
<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Alberta" target="_blank">Parts Registry</a><br />
<br />
</div><br />
</li><br />
<li><br />
<a>Notebook</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Calendar">Lab Notebook</a><br />
<a href="https://2009.igem.org/Team:Alberta/Blackboard">Lab Blackboard</a><br />
<a href="https://2009.igem.org/Team:Alberta/Protocols">Protocols</a><br />
<a href="https://2009.igem.org/Team:Alberta/Links">Links</a><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/References/Keyword">References</a><br />
</li><br />
<br />
</ul><br />
<br />
<br />
</div><br />
<br />
<!-- END main navigation --><br />
<br />
</div><br />
</center><br />
</html><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br />
{{:Team:Alberta/LeftBar}}<br />
<br />
<html><br />
<style type="text/css"><br />
body {background-color:#f8f8f8;}<br />
</style><br />
</html></div>
Wiltshir
http://2009.igem.org/Team:Alberta/Template3
Team:Alberta/Template3
2009-10-20T08:03:54Z
<p>Wiltshir: </p>
<hr />
<div><html><br />
<title>University of Alberta - BioBytes</title><br />
<br />
<link rel="stylesheet" type="text/css" href="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/main.css&action=raw&ctype=text/css" /><br />
<link rel="stylesheet" type="text/css" href="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/dd.css&action=raw&ctype=text/css" /><br />
<!--[if IE]><br />
<style type="text/css"><br />
#utilNav ul li {list-style-image: none;}<br />
#suppNav ul li {list-style-image: none;}<br />
#mainNav ul li {list-style-image: none;}<br />
</style><br />
<![endif]--><br />
<script type="text/javascript" src="https://2009.igem.org/wiki/index.php?title=User:Home_World/wiki/java.js&action=raw&ctype=text/javascript"></script><br />
<script type="text/javascript"><br />
function ddnav() {<br />
$('#ddNav li').hover(<br />
function () {<br />
$(this).find('div:first').css('display','block');},<br />
function () {<br />
$(this).find('div:first').css('display','none');}<br />
);<br />
}<br />
function ddnavsub() {<br />
$('#ddNav span > a').toggle(<br />
function () {<br />
$(this).removeClass("expand").addClass("collapse");<br />
$(this).parent().find('div:first').slideDown('fast');<br />
$(this).hover(<br />
function (){$(this).css('background-color','#e5e5e0')},<br />
function (){$(this).css('background-color','#e5e5e0')});},<br />
function () {<br />
$(this).removeClass("collapse").addClass("expand");<br />
$(this).parent().find('div:first').css('display','none');<br />
$(this).hover(<br />
function (){$(this).css('background-color','#F5F5F0')},<br />
function (){$(this).css('background-color','#FFFFFF')});}<br />
).addClass('expand');<br />
}<br />
<br />
function ddmozilla() {<br />
ddtoggle();<br />
}<br />
<br />
$(function () {<br />
ddnav();<br />
ddnavsub();<br />
<br />
if($.browser.mozilla) {ddmozilla();}<br />
});<br />
</script><br />
<center><br />
<div id="header"><br />
<img src="https://static.igem.org/mediawiki/2009/0/04/UofA_biobytes_HeaderBanner5.png" width="965" height="235"><br />
<br />
<!-- logo --><br />
<div id="logo"><br />
</div><br />
<!-- END logo --><br />
<br />
<!-- main navigation --><br />
<div id="mainNav"><br />
<ul id="ddNav"><br />
<br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta">Home</a><br />
</li><br />
<li><br />
<a>Team</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Team">Undergraduates</a><br />
<a href="https://2009.igem.org/Team:Alberta/Team#Team_Ad">Team Advisors</a><br />
<a href="https://2009.igem.org/Team:Alberta/Team#Fac_Con">Faculty Consultants</a><br />
</div><br />
</li><br />
<li><br />
<a>Project</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Bioinformatics">Bioinformatics</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/assemblyoverview">Assembly Process</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Recombineering">Recombineering</a><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Outreach">Outreach</a><br />
</div><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/MedalRequirements">Deliverables</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/MedalRequirements">Deliverables</a><br />
<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Alberta" target="_blank">Parts Registry</a><br />
<br />
</div><br />
</li><br />
<li><br />
<a>Notebook</a><br />
<div><br />
<a href="https://2009.igem.org/Team:Alberta/Calendar">Lab Notebook</a><br />
<a href="https://2009.igem.org/Team:Alberta/Blackboard">Lab Blackboard</a><br />
<a href="https://2009.igem.org/Team:Alberta/Protocols">Protocols</a><br />
<a href="https://2009.igem.org/Team:Alberta/Links">Links</a><br />
</li><br />
<li><br />
<a href="https://2009.igem.org/Team:Alberta/References/Keyword">References</a><br />
</li><br />
<li><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
<!-- END main navigation --><br />
<br />
</div><br />
</center><br />
</html><br />
<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br />
{{:Team:Alberta/LeftBar}}<br />
{{:Team:Alberta/RightBar}}<br />
<html><br />
<style type="text/css"><br />
body {background-color:#f8f8f8;}<br />
</style><br />
</html></div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Keyword
Team:Alberta/References/Keyword
2009-10-20T08:00:01Z
<p>Wiltshir: </p>
<hr />
<div>{{:Team:Alberta/Template3}}<br />
<html><br />
<head><br />
<style type="text/css"><br />
.b1f, .b2f, .b3f, .b4f{font-size:1px; overflow:hidden; display:block;}<br />
.b1f {height:1px; background:#e1e1e1; margin:0 5px;}<br />
.b2f {height:1px; background:#e1e1e1; margin:0 3px;}<br />
.b3f {height:1px; background:#e1e1e1; margin:0 2px;}<br />
.b4f {height:2px; background:#e1e1e1; margin:0 1px;}<br />
.content {background: #e1e1e1;}<br />
.content div {margin-left: 5px;}<br />
</style><br />
</head><br />
<br />
<div class="all"><br />
<div style="background:#FFFFFF"><br />
<br />
<!-- adjust table width, main background and padding between cells and edge of background --><br />
<br />
<br />
<table width=60% style="background:#FFFFFF; padding:2px;"><br />
<br />
<tr><br />
<td style="height: 400; padding-left: 10px; padding-right: 10px; padding-top: 11px;"><br />
<b class="b1f"></b><b class="b2f"></b><b class="b3f"></b><b class="b4f"></b><br />
<div class="Recoli"><br />
<div style="height: 400; background:#FFFFFF; colorou line-height:100% padding: 3px 0px;"><br />
<h1>References</h1><br />
<br />
<h2>Bioinformatics</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/BioWarehouse: a bioinformatics database warehouse toolkit" target="_blank">BioWarehouse: a bioinformatics database warehouse toolkit</a><br />
Lee (2006)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Triad pattern algorithms for predicting strong promoter candidates in bacterial genomes" target="_blank">Triad pattern algorithms for predicting strong promoter candidates in bacterial genomes</a><br />
Dekhtyar (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Vector NTI, a balanced all-in-one sequence analysis suite" target="_blank">Vector NTI, a balanced all-in-one sequence analysis suite</a><br />
Lu (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Modeling" target="_blank">E. Coli Metabolic Modelling in Matlab</a><br />
</p><br />
<br />
<h2>Deletion</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda red" target="_blank">An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda red</a><br />
Fukiya (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome" target="_blank">Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome</a><br />
Hashimoto (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Construction of consecutive deletions of the Escherichia coli chromosome" target="_blank">Construction of consecutive deletions of the Escherichia coli chromosome</a><br />
Kato (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Construction of Escherichia coli K-12 in-frame, single gene knockout mutants: the Keio collection" target="_blank">Construction of Escherichia coli K-12 in-frame, single gene knockout mutants: the Keio collection</a><br />
Baba (2006)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome" target="_blank">Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome</a><br />
Kato (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Emergent properties of reduced genome Escherichia coli" target="_blank">Emergent properties of reduced genome Escherichia coli</a><br />
Posfai (2006)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Engineering a reduced Escherichia coli genome" target="_blank">Engineering a reduced Escherichia coli genome</a><br />
Kolisnychenko (2002)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Rapid and efficient construction of markerless deletions in the Escherichia coli genome" target="_blank">Rapid and efficient construction of markerless deletions in the Escherichia coli genome</a><br />
Yu (2008) <br />
</p><br />
<br />
<h2>DNA Assembly</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium" target="_blank">Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium</a><br />
Gibson (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine" target="_blank">DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine</a><br />
Kelman (1995)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/DNA Replication" target="_blank">DNA Replication</a><br />
Kornberg (1988)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Enzymatic assembly of DNA molecules up to several hundred kilobases" target="_blank">Enzymatic assembly of DNA molecules up to several hundred kilobases</a><br />
Gibson (2009)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Rebuilding microbial genomes" target="_blank">Rebuilding microbial genomes</a><br />
Holt (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Refactoring bacteriophage T7" target="_blank">Refactoring bacteriophage T7</a><br />
Chan (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/The replication-related organization of bacterial genomes" target="_blank">The replication-related organization of bacterial genomes</a><br />
Rocha (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Shear induced assembly of lambda phage DNA" target="_blank">Shear induced assembly of lambda phage DNA</a><br />
Haber (2000)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Structural basis for uracil recognition by archaeal family B DNA polymerases" target="_blank">Structural basis for uracil recognition by archaeal family B DNA polymerases</a><br />
Fogg (2002)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/USERTM friendly DNA engineering and cloning method by uracil excision" target="_blank">USERTM friendly DNA engineering and cloning method by uracil excision</a><br />
Bitinaite (2007) <br />
</p><br />
<br />
<h2>E. coli Physiology</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome" target="_blank">Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome</a><br />
Hashimoto (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Escherichia coli physiology in Luria-Bertani broth" target="_blank">Escherichia coli physiology in Luria-Bertani broth</a><br />
Sezonov (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Fermentation pathways of Escherichia coli, The" target="_blank">Fermentation pathways of Escherichia coli, The</a><br />
Clark (1989)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110" target="_blank">Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110</a><br />
Hayashi (2006)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/The replication-related organization of bacterial genomes" target="_blank">The replication-related organization of bacterial genomes</a><br />
Rocha (2004) <br />
</p><br />
<br />
<h2>Essential Genes</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/The adjacent dnaZ and dnaX genes of Escherichia coli are contained within one continuous open reading frame" target="_blank">The adjacent dnaZ and dnaX genes of Escherichia coli are contained within one continuous open reading frame</a><br />
Flower (1986)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Chromosomal Locations of the Genes for rRNA in Escherichia coli K-12t" target="_blank">Chromosomal Locations of the Genes for rRNA in Escherichia coli K-12t</a><br />
Ellwood (1982)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Construction of Escherichia coli K-12 in-frame, single gene knockout mutants: the Keio collection" target="_blank">Construction of Escherichia coli K-12 in-frame, single gene knockout mutants: the Keio collection</a><br />
Baba (2006)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Complete set of ORF clones of Escherichia coli ASKA library (a complete set of Escherichia coli K-12 ORF archive): unique resources for biological research" target="_blank">Complete set of ORF clones of Escherichia coli ASKA library (a complete set of Escherichia coli K-12 ORF archive): unique resources for biological research</a><br />
Kitagawa (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Determination of the core of a minimal bacterial gene set" target="_blank">Determination of the core of a minimal bacterial gene set</a><br />
Gil (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine" target="_blank">DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine</a><br />
Kelman (1995)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Experimental determination and systems level analysis of essential genes in Escherichia coli MG1665" target="_blank">Experimental determination and systems level analysis of essential genes in Escherichia coli MG1665</a><br />
Gerdes (2003)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/The Heat-Shock-Regulated grpE Gene of Escherichia coli Is Required for Bacterial Growth at All Temperatures but Is Dispensable in Certain Mutant Backgrounds" target="_blank">The Heat-Shock-Regulated grpE Gene of Escherichia coli Is Required for Bacterial Growth at All Temperatures but Is Dispensable in Certain Mutant Backgrounds</a><br />
Ang (1989)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Operon Structure of dnaT and dnaC Genes Essential for Normal and Stable DNA Replication of Escherichia coli Chromosome" target="_blank">Operon Structure of dnaT and dnaC Genes Essential for Normal and Stable DNA Replication of Escherichia coli Chromosome</a><br />
Masai (1988)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Site-directed Mutational Analysis for the ATP Binding of DnaA Protein" target="_blank">Site-directed Mutational Analysis for the ATP Binding of DnaA Protein</a><br />
Mizushima (1998) <br />
</p><br />
<br />
<h2>Genes, Other</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Coding-sequence determinants of gene expression in Escherichia coli" target="_blank">Coding-sequence determinants of gene expression in Escherichia coli</a><br />
Kudla (2009)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Compilation and analysis of Escherichia coli promoter DNA sequences" target="_blank">Compilation and analysis of Escherichia coli promoter DNA sequences</a><br />
Hawley (1983)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Effect of dna Mutations on the Replication of Plasmid pSC101 in Escherichia coli K-12" target="_blank">Effect of dna Mutations on the Replication of Plasmid pSC101 in Escherichia coli K-12</a><br />
Hasunuma (1979)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Genome-wide expression profiling in Escherichia coli K-12" target="_blank">Genome-wide expression profiling in Escherichia coli K-12</a><br />
Richmond (1999)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110" target="_blank">Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110</a><br />
Hayashi (2006)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Idempotent Vector Design for Standard Assembly of Biobricks" target="_blank">Idempotent Vector Design for Standard Assembly of Biobricks</a><br />
Knight 2003<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli" target="_blank">Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli</a><br />
Morgan (1977)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Programming gene expression with combinatorial promoters" target="_blank">Programming gene expression with combinatorial promoters</a><br />
Cox (2007) <br />
</p><br />
<br />
<h2>Microfluidics</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/An inexpensive and portable microchip-based platform for integrated<br />
RT–PCR and capillary electrophoresis" target="_blank">An inexpensive and portable microchip-based platform for integrated<br />
RT–PCR and capillary electrophoresis</a><br />
Kaigala (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/An integrated microfluidic biochemical detection system forprotein analysis with magnetic bead-based samplingcapabilities" target="_blank">An integrated microfluidic biochemical detection system forprotein analysis with magnetic bead-based sampling capabilities</a><br />
Choi (2001)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Development of a microfluidic biosensor module for pathogen detection" target="_blank">Development of a microfluidic biosensor module for pathogen detection</a><br />
Zaytseva (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Magnetic bead handling on-chip: new opportunities for analytical applications" target="_blank">Magnetic bead handling on-chip: new opportunities for analytical applications </a><br />
Gijs (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Microelectromagnet for magnetic manipulation in lab-on-a-chip systems" target="_blank">Microelectromagnet for magnetic manipulation in lab-on-a-chip systems</a><br />
Smistrup (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Microfabricated Device for DNA and RNA Amplification by Continuous-Flow Polymerase Chain Reaction and Reverse Transcription-Polymerase Chain Reaction with Cycle Number Selection" target="_blank">Microfabricated Device for DNA and RNA Amplification by Continuous-Flow Polymerase Chain Reaction and Reverse Transcription-Polymerase Chain Reaction with Cycle Number Selection</a><br />
Obeid (2003)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Nucleic acid purification using microfabricated silicon structures" target="_blank">Nucleic acid purification using microfabricated silicon structures</a><br />
Sun (250)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/On-chip oligonucleotide ligation assay using one-dimensional microfluidic beads array for the detection of low-abundant DNA point mutations" target="_blank">On-chip oligonucleotide ligation assay using one-dimensional microfluidic beads array for the detection of low-abundant DNA point mutations </a><br />
Zhang (2005)<br />
</p><br />
<br />
<h2>Nucleic Acid Properties</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Binding of biotinylated DNA to streptavidin-coated polystyrene latex: effects of chain length and particle size" target="_blank">Binding of biotinylated DNA to streptavidin-coated polystyrene latex: effects of chain length and particle size</a><br />
Huang (1996)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Dynamic strength of molecular adhesion bonds" target="_blank">Dynamic strength of molecular adhesion bonds</a><br />
Evans (1997)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/The effect of hydrodynamic shear of DNA from T2 and T4 bacteriophages" target="_blank">The effect of hydrodynamic shear of DNA from T2 and T4 bacteriophages</a><br />
Davison (1959)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Effect of shear on plasmid DNA in solution" target="_blank">Effect of shear on plasmid DNA in solution</a><br />
Levy (1999)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Folding and unfolding single RNA molecules under tension" target="_blank">Folding and unfolding single RNA molecules under tension</a><br />
Woodside (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays" target="_blank">Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays</a><br />
Bernstein (2002)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Shear breakage of DNA" target="_blank">Shear breakage of DNA</a><br />
Dancis (1978)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Shear degradation of DNA" target="_blank">Shear degradation of DNA</a><br />
Adam (1977)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Shear-induced degradation of plasmid DNA" target="_blank">Shear-induced degradation of plasmid DNA</a><br />
Lengsfeld (2002)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Shear unzipping of DNA" target="_blank">Shear unzipping of DNA</a><br />
Chakrabarti (2009)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Stochastic simulations of DNA in flow: dynamics and the effects of hydrodynamic interactions" target="_blank">Stochastic simulations of DNA in flow: dynamics and the effects of hydrodynamic interactions</a><br />
Jendrejack (2002) <br />
</p><br />
<br />
<h2>Operons</h2><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Engineering of bacterial ribosomes: Replacement of all seven Escherichia coli rRNA operons by a single plasmid-encoded operon" target="_blank">Engineering of bacterial ribosomes: Replacement of all seven Escherichia coli rRNA operons by a single plasmid-encoded operon</a><br />
Nomura (1999)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli" target="_blank">Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli</a><br />
Morgan (1977)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Operon Structure of dnaT and dnaC Genes Essential for Normal and Stable DNA Replication of Escherichia coli Chromosome" target="_blank">Operon Structure of dnaT and dnaC Genes Essential for Normal and Stable DNA Replication of Escherichia coli Chromosome</a><br />
Masai (1988) <br />
</p><br />
<br />
<h2>Recombination</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda red" target="_blank">An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda red</a><br />
Fukiya (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Biochemistry of homologous recombination in Escherichia coli" target="_blank">Biochemistry of homologous recombination in Escherichia coli</a><br />
Kowalczykowski (1994)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome" target="_blank">Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome</a><br />
Hashimoto (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector" target="_blank">Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector</a><br />
Shizuya (1992)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Conditional-Replication, Integration, Excision, and Retrieval Plasmid-Host Systems for Gene Structure-Function Studies of Bacteria" target="_blank">Conditional-Replication, Integration, Excision, and Retrieval Plasmid-Host Systems for Gene Structure-Function Studies of Bacteria</a><br />
Haldimann (2001)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Construction of consecutive deletions of the Escherichia coli chromosome" target="_blank">Construction of consecutive deletions of the Escherichia coli chromosome</a><br />
Kato (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome" target="_blank">Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome</a><br />
Kato (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/DNA cloning by homologous recombination in Escherichia coli" target="_blank">DNA cloning by homologous recombination in Escherichia coli</a><br />
Zhang (2000)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Engineering a reduced Escherichia coli genome" target="_blank">Engineering a reduced Escherichia coli genome</a><br />
Kolisnychenko (2002)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Gene replacement without selection: regulated suppression of amber mutations in Escherichia coli" target="_blank">Gene replacement without selection: regulated suppression of amber mutations in Escherichia coli</a><br />
Herring (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Gene splicing and mutagenesis by PCR driven overlap extension" target="_blank">Gene splicing and mutagenesis by PCR driven overlap extension</a><br />
Heckman (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/In vivo transposition of mariner based elements in enteric bacteria and mycobacteria" target="_blank">In vivo transposition of mariner based elements in enteric bacteria and mycobacteria</a><br />
Rubin (1999)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Markerless gene replacement in Escherichia coli stimulated by double strand break in the chromosome" target="_blank">Markerless gene replacement in Escherichia coli stimulated by double strand break in the chromosome</a><br />
Posfai (1999)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/One step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome" target="_blank">One step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome</a><br />
Gibson (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products" target="_blank">One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products</a><br />
Datsenko (2000)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Phage integrases: biology and applications" target="_blank">Phage integrases: biology and applications</a><br />
Groth (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Rapid and efficient construction of markerless deletions in the Escherichia coli genome" target="_blank">Rapid and efficient construction of markerless deletions in the Escherichia coli genome</a><br />
Yu (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Recombineering with tolC as a selectable/counter-selectable marker: remodeling the rRNA operons in Escherichia coli" target="_blank">Recombineering with tolC as a selectable/counter-selectable marker: remodeling the rRNA operons in Escherichia coli</a><br />
DeVito (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Scarless engineering of the Escherichia coli genome" target="_blank">Scarless engineering of the Escherichia coli genome</a><br />
Feher (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Shear induced assembly of lambda phage DNA" target="_blank">Shear induced assembly of lambda phage DNA</a><br />
Haber (2000)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Simple and highly efficient BAC recombineering using galK selection" target="_blank">Simple and highly efficient BAC recombineering using galK selection</a><br />
Warming (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Techniques: recombinogenic engineering - new options for cloning and manipulating DNA" target="_blank">Techniques: recombinogenic engineering - new options for cloning and manipulating DNA</a><br />
Muyrers (2001)<br />
</p><br />
<br />
<h2>Synthetic Biology</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Engineering BioBrick vectors from BioBrick parts" target="_blank">Engineering BioBrick vectors from BioBrick parts</a><br />
Shetty (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Idempotent Vector Design for Standard Assembly of Biobricks" target="_blank">Idempotent Vector Design for Standard Assembly of Biobricks</a><br />
Knight 2003<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Refactoring bacteriophage T7" target="_blank">Refactoring bacteriophage T7</a><br />
Chan (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Refinement and standardization of synthetic biological parts and devices" target="_blank">Refinement and standardization of synthetic biological parts and devices</a><br />
Canton (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Setting the standard in synthetic biology" target="_blank">Setting the standard in synthetic biology</a><br />
Arkin (2008) <br />
</p><br />
<br />
<h2>Transformation</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Transformation of Escherichia coli with large DNA molecules by electroporation" target="_blank">Transformation of Escherichia coli with large DNA molecules by electroporation</a><br />
Sheng (1995) <br />
</p><br />
<br />
<h2>Vectors</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/A family of LIC vectors for high through-put cloning and purification of proteins" target="_blank">A family of LIC vectors for high through-put cloning and purification of proteins</a><br />
Eschenfeldt (2009)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector" target="_blank">Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector</a><br />
Shizuya (1992)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Effect of dna Mutations on the Replication of Plasmid pSC101 in Escherichia coli K-12" target="_blank">Effect of dna Mutations on the Replication of Plasmid pSC101 in Escherichia coli K-12</a><br />
Hasunuma (1979)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Engineering BioBrick vectors from BioBrick parts" target="_blank">Engineering BioBrick vectors from BioBrick parts</a><br />
Shetty (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Identification of the mob Genes of Plasmid pSC101 and Characterization of a Hybrid pSC101-R1162 System for Conjugal Mobilization" target="_blank">Identification of the mob Genes of Plasmid pSC101 and Characterization of a Hybrid pSC101-R1162 System for Conjugal Mobilization</a><br />
Meyer (2000)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Primary structure of the essential replicon of the plasmid pSC101" target="_blank">Primary structure of the essential replicon of the plasmid pSC101</a><br />
Vocke (1983)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Replication of plasmid pSC101" target="_blank">Replication of plasmid pSC101</a><br />
Manen (1991)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Simple and highly efficient BAC recombineering using galK selection" target="_blank">Simple and highly efficient BAC recombineering using galK selection</a><br />
Warming (2005) <br />
</p><br />
<br />
<br />
<!-- <div align="justify" style="padding-left:20px; padding-right:20px"> --><br />
<div align="justify"><br />
<br />
<br />
</div><br />
</div></div><br />
<b class="b4f"></b><b class="b3f"></b><b class="b2f"></b><b class="b1f"></b><br />
</td><br />
</tr><br />
</table><br />
</div><br />
</HTML></div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Keyword
Team:Alberta/References/Keyword
2009-10-20T07:41:24Z
<p>Wiltshir: Addition of target="_blank" tag</p>
<hr />
<div>{{:Team:Alberta/Template3}}<br />
<html><br />
<head><br />
<style type="text/css"><br />
.b1f, .b2f, .b3f, .b4f{font-size:1px; overflow:hidden; display:block;}<br />
.b1f {height:1px; background:#e1e1e1; margin:0 5px;}<br />
.b2f {height:1px; background:#e1e1e1; margin:0 3px;}<br />
.b3f {height:1px; background:#e1e1e1; margin:0 2px;}<br />
.b4f {height:2px; background:#e1e1e1; margin:0 1px;}<br />
.content {background: #e1e1e1;}<br />
.content div {margin-left: 5px;}<br />
</style><br />
</head><br />
<br />
<div class="all"><br />
<div style="background:#FFFFFF"><br />
<br />
<!-- adjust table width, main background and padding between cells and edge of background --><br />
<br />
<br />
<table width=60% style="background:#FFFFFF; padding:2px;"><br />
<br />
<tr><br />
<td style="height: 400; padding-left: 10px; padding-right: 10px; padding-top: 11px;"><br />
<b class="b1f"></b><b class="b2f"></b><b class="b3f"></b><b class="b4f"></b><br />
<div class="Recoli"><br />
<div style="height: 400; background:#FFFFFF; colorou line-height:100% padding: 3px 0px;"><br />
<h1>References</h1><br />
<br />
<h2>Bioinformatics</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/BioWarehouse: a bioinformatics database warehouse toolkit" target="_blank">BioWarehouse: a bioinformatics database warehouse toolkit</a><br />
Lee (2006)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Triad pattern algorithms for predicting strong promoter candidates in bacterial genomes" target="_blank">Triad pattern algorithms for predicting strong promoter candidates in bacterial genomes</a><br />
Dekhtyar (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Vector NTI, a balanced all-in-one sequence analysis suite" target="_blank">Vector NTI, a balanced all-in-one sequence analysis suite</a><br />
Lu (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Modeling" target="_blank">E. Coli Metabolic Modelling in Matlab</a><br />
</p><br />
<br />
<h2>Deletion</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda red" target="_blank">An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda red</a><br />
Fukiya (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome" target="_blank">Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome</a><br />
Hashimoto (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Construction of consecutive deletions of the Escherichia coli chromosome" target="_blank">Construction of consecutive deletions of the Escherichia coli chromosome</a><br />
Kato (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Construction of Escherichia coli K-12 in-frame, single gene knockout mutants: the Keio collection" target="_blank">Construction of Escherichia coli K-12 in-frame, single gene knockout mutants: the Keio collection</a><br />
Baba (2006)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome" target="_blank">Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome</a><br />
Kato (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Emergent properties of reduced genome Escherichia coli" target="_blank">Emergent properties of reduced genome Escherichia coli</a><br />
Posfai (2006)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Engineering a reduced Escherichia coli genome" target="_blank">Engineering a reduced Escherichia coli genome</a><br />
Kolisnychenko (2002)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Rapid and efficient construction of markerless deletions in the Escherichia coli genome" target="_blank">Rapid and efficient construction of markerless deletions in the Escherichia coli genome</a><br />
Yu (2008) <br />
</p><br />
<br />
<h2>DNA Assembly</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium" target="_blank">Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium</a><br />
Gibson (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine" target="_blank">DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine</a><br />
Kelman (1995)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/DNA Replication" target="_blank">DNA Replication</a><br />
Kornberg (1988)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Enzymatic assembly of DNA molecules up to several hundred kilobases" target="_blank">Enzymatic assembly of DNA molecules up to several hundred kilobases</a><br />
Gibson (2009)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Rebuilding microbial genomes" target="_blank">Rebuilding microbial genomes</a><br />
Holt (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Refactoring bacteriophage T7" target="_blank">Refactoring bacteriophage T7</a><br />
Chan (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/The replication-related organization of bacterial genomes" target="_blank">The replication-related organization of bacterial genomes</a><br />
Rocha (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Shear induced assembly of lambda phage DNA" target="_blank">Shear induced assembly of lambda phage DNA</a><br />
Haber (2000)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Structural basis for uracil recognition by archaeal family B DNA polymerases" target="_blank">Structural basis for uracil recognition by archaeal family B DNA polymerases</a><br />
Fogg (2002)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/USERTM friendly DNA engineering and cloning method by uracil excision" target="_blank">USERTM friendly DNA engineering and cloning method by uracil excision</a><br />
Bitinaite (2007) <br />
</p><br />
<br />
<h2>E. coli Physiology</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome" target="_blank">Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome</a><br />
Hashimoto (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Escherichia coli physiology in Luria-Bertani broth" target="_blank">Escherichia coli physiology in Luria-Bertani broth</a><br />
Sezonov (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Fermentation pathways of Escherichia coli, The" target="_blank">Fermentation pathways of Escherichia coli, The</a><br />
Clark (1989)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110" target="_blank">Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110</a><br />
Hayashi (2006)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/The replication-related organization of bacterial genomes" target="_blank">The replication-related organization of bacterial genomes</a><br />
Rocha (2004) <br />
</p><br />
<br />
<h2>Essential Genes</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/The adjacent dnaZ and dnaX genes of Escherichia coli are contained within one continuous open reading frame" target="_blank">The adjacent dnaZ and dnaX genes of Escherichia coli are contained within one continuous open reading frame</a><br />
Flower (1986)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Chromosomal Locations of the Genes for rRNA in Escherichia coli K-12t" target="_blank">Chromosomal Locations of the Genes for rRNA in Escherichia coli K-12t</a><br />
Ellwood (1982)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Construction of Escherichia coli K-12 in-frame, single gene knockout mutants: the Keio collection" target="_blank">Construction of Escherichia coli K-12 in-frame, single gene knockout mutants: the Keio collection</a><br />
Baba (2006)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Complete set of ORF clones of Escherichia coli ASKA library (a complete set of Escherichia coli K-12 ORF archive): unique resources for biological research" target="_blank">Complete set of ORF clones of Escherichia coli ASKA library (a complete set of Escherichia coli K-12 ORF archive): unique resources for biological research</a><br />
Kitagawa (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Determination of the core of a minimal bacterial gene set" target="_blank">Determination of the core of a minimal bacterial gene set</a><br />
Gil (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine" target="_blank">DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine</a><br />
Kelman (1995)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Experimental determination and systems level analysis of essential genes in Escherichia coli MG1665" target="_blank">Experimental determination and systems level analysis of essential genes in Escherichia coli MG1665</a><br />
Gerdes (2003)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/The Heat-Shock-Regulated grpE Gene of Escherichia coli Is Required for Bacterial Growth at All Temperatures but Is Dispensable in Certain Mutant Backgrounds" target="_blank">The Heat-Shock-Regulated grpE Gene of Escherichia coli Is Required for Bacterial Growth at All Temperatures but Is Dispensable in Certain Mutant Backgrounds</a><br />
Ang (1989)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Operon Structure of dnaT and dnaC Genes Essential for Normal and Stable DNA Replication of Escherichia coli Chromosome" target="_blank">Operon Structure of dnaT and dnaC Genes Essential for Normal and Stable DNA Replication of Escherichia coli Chromosome</a><br />
Masai (1988)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Site-directed Mutational Analysis for the ATP Binding of DnaA Protein" target="_blank">Site-directed Mutational Analysis for the ATP Binding of DnaA Protein</a><br />
Mizushima (1998) <br />
</p><br />
<br />
<h2>Genes, Other</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Coding-sequence determinants of gene expression in Escherichia coli" target="_blank">Coding-sequence determinants of gene expression in Escherichia coli</a><br />
Kudla (2009)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Compilation and analysis of Escherichia coli promoter DNA sequences" target="_blank">Compilation and analysis of Escherichia coli promoter DNA sequences</a><br />
Hawley (1983)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Effect of dna Mutations on the Replication of Plasmid pSC101 in Escherichia coli K-12" target="_blank">Effect of dna Mutations on the Replication of Plasmid pSC101 in Escherichia coli K-12</a><br />
Hasunuma (1979)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Genome-wide expression profiling in Escherichia coli K-12" target="_blank">Genome-wide expression profiling in Escherichia coli K-12</a><br />
Richmond (1999)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110" target="_blank">Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110</a><br />
Hayashi (2006)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Idempotent Vector Design for Standard Assembly of Biobricks" target="_blank">Idempotent Vector Design for Standard Assembly of Biobricks</a><br />
Knight 2003<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli" target="_blank">Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli</a><br />
Morgan (1977)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Programming gene expression with combinatorial promoters" target="_blank">Programming gene expression with combinatorial promoters</a><br />
Cox (2007) <br />
</p><br />
<br />
<h2>Microfluidics</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/An inexpensive and portable microchip-based platform for integrated<br />
RT–PCR and capillary electrophoresis" target="_blank">An inexpensive and portable microchip-based platform for integrated<br />
RT–PCR and capillary electrophoresis</a><br />
Kaigala (2008)<br />
</p><br />
<br />
<h2>Nucleic Acid Properties</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Binding of biotinylated DNA to streptavidin-coated polystyrene latex: effects of chain length and particle size" target="_blank">Binding of biotinylated DNA to streptavidin-coated polystyrene latex: effects of chain length and particle size</a><br />
Huang (1996)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Dynamic strength of molecular adhesion bonds" target="_blank">Dynamic strength of molecular adhesion bonds</a><br />
Evans (1997)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/The effect of hydrodynamic shear of DNA from T2 and T4 bacteriophages" target="_blank">The effect of hydrodynamic shear of DNA from T2 and T4 bacteriophages</a><br />
Davison (1959)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Effect of shear on plasmid DNA in solution" target="_blank">Effect of shear on plasmid DNA in solution</a><br />
Levy (1999)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Folding and unfolding single RNA molecules under tension" target="_blank">Folding and unfolding single RNA molecules under tension</a><br />
Woodside (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays" target="_blank">Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays</a><br />
Bernstein (2002)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Shear breakage of DNA" target="_blank">Shear breakage of DNA</a><br />
Dancis (1978)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Shear degradation of DNA" target="_blank">Shear degradation of DNA</a><br />
Adam (1977)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Shear-induced degradation of plasmid DNA" target="_blank">Shear-induced degradation of plasmid DNA</a><br />
Lengsfeld (2002)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Shear unzipping of DNA" target="_blank">Shear unzipping of DNA</a><br />
Chakrabarti (2009)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Stochastic simulations of DNA in flow: dynamics and the effects of hydrodynamic interactions" target="_blank">Stochastic simulations of DNA in flow: dynamics and the effects of hydrodynamic interactions</a><br />
Jendrejack (2002) <br />
</p><br />
<br />
<h2>Operons</h2><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Engineering of bacterial ribosomes: Replacement of all seven Escherichia coli rRNA operons by a single plasmid-encoded operon" target="_blank">Engineering of bacterial ribosomes: Replacement of all seven Escherichia coli rRNA operons by a single plasmid-encoded operon</a><br />
Nomura (1999)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli" target="_blank">Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli</a><br />
Morgan (1977)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Operon Structure of dnaT and dnaC Genes Essential for Normal and Stable DNA Replication of Escherichia coli Chromosome" target="_blank">Operon Structure of dnaT and dnaC Genes Essential for Normal and Stable DNA Replication of Escherichia coli Chromosome</a><br />
Masai (1988) <br />
</p><br />
<br />
<h2>Recombination</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda red" target="_blank">An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda red</a><br />
Fukiya (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Biochemistry of homologous recombination in Escherichia coli" target="_blank">Biochemistry of homologous recombination in Escherichia coli</a><br />
Kowalczykowski (1994)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome" target="_blank">Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome</a><br />
Hashimoto (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector" target="_blank">Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector</a><br />
Shizuya (1992)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Conditional-Replication, Integration, Excision, and Retrieval Plasmid-Host Systems for Gene Structure-Function Studies of Bacteria" target="_blank">Conditional-Replication, Integration, Excision, and Retrieval Plasmid-Host Systems for Gene Structure-Function Studies of Bacteria</a><br />
Haldimann (2001)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Construction of consecutive deletions of the Escherichia coli chromosome" target="_blank">Construction of consecutive deletions of the Escherichia coli chromosome</a><br />
Kato (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome" target="_blank">Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome</a><br />
Kato (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/DNA cloning by homologous recombination in Escherichia coli" target="_blank">DNA cloning by homologous recombination in Escherichia coli</a><br />
Zhang (2000)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Engineering a reduced Escherichia coli genome" target="_blank">Engineering a reduced Escherichia coli genome</a><br />
Kolisnychenko (2002)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Gene replacement without selection: regulated suppression of amber mutations in Escherichia coli" target="_blank">Gene replacement without selection: regulated suppression of amber mutations in Escherichia coli</a><br />
Herring (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Gene splicing and mutagenesis by PCR driven overlap extension" target="_blank">Gene splicing and mutagenesis by PCR driven overlap extension</a><br />
Heckman (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/In vivo transposition of mariner based elements in enteric bacteria and mycobacteria" target="_blank">In vivo transposition of mariner based elements in enteric bacteria and mycobacteria</a><br />
Rubin (1999)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Markerless gene replacement in Escherichia coli stimulated by double strand break in the chromosome" target="_blank">Markerless gene replacement in Escherichia coli stimulated by double strand break in the chromosome</a><br />
Posfai (1999)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/One step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome" target="_blank">One step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome</a><br />
Gibson (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products" target="_blank">One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products</a><br />
Datsenko (2000)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Phage integrases: biology and applications" target="_blank">Phage integrases: biology and applications</a><br />
Groth (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Rapid and efficient construction of markerless deletions in the Escherichia coli genome" target="_blank">Rapid and efficient construction of markerless deletions in the Escherichia coli genome</a><br />
Yu (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Recombineering with tolC as a selectable/counter-selectable marker: remodeling the rRNA operons in Escherichia coli" target="_blank">Recombineering with tolC as a selectable/counter-selectable marker: remodeling the rRNA operons in Escherichia coli</a><br />
DeVito (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Scarless engineering of the Escherichia coli genome" target="_blank">Scarless engineering of the Escherichia coli genome</a><br />
Feher (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Shear induced assembly of lambda phage DNA" target="_blank">Shear induced assembly of lambda phage DNA</a><br />
Haber (2000)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Simple and highly efficient BAC recombineering using galK selection" target="_blank">Simple and highly efficient BAC recombineering using galK selection</a><br />
Warming (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Techniques: recombinogenic engineering - new options for cloning and manipulating DNA" target="_blank">Techniques: recombinogenic engineering - new options for cloning and manipulating DNA</a><br />
Muyrers (2001)<br />
</p><br />
<br />
<h2>Synthetic Biology</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Engineering BioBrick vectors from BioBrick parts" target="_blank">Engineering BioBrick vectors from BioBrick parts</a><br />
Shetty (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Idempotent Vector Design for Standard Assembly of Biobricks" target="_blank">Idempotent Vector Design for Standard Assembly of Biobricks</a><br />
Knight 2003<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Refactoring bacteriophage T7" target="_blank">Refactoring bacteriophage T7</a><br />
Chan (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Refinement and standardization of synthetic biological parts and devices" target="_blank">Refinement and standardization of synthetic biological parts and devices</a><br />
Canton (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Setting the standard in synthetic biology" target="_blank">Setting the standard in synthetic biology</a><br />
Arkin (2008) <br />
</p><br />
<br />
<h2>Transformation</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Transformation of Escherichia coli with large DNA molecules by electroporation" target="_blank">Transformation of Escherichia coli with large DNA molecules by electroporation</a><br />
Sheng (1995) <br />
</p><br />
<br />
<h2>Vectors</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/A family of LIC vectors for high through-put cloning and purification of proteins" target="_blank">A family of LIC vectors for high through-put cloning and purification of proteins</a><br />
Eschenfeldt (2009)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector" target="_blank">Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector</a><br />
Shizuya (1992)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Effect of dna Mutations on the Replication of Plasmid pSC101 in Escherichia coli K-12" target="_blank">Effect of dna Mutations on the Replication of Plasmid pSC101 in Escherichia coli K-12</a><br />
Hasunuma (1979)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Engineering BioBrick vectors from BioBrick parts" target="_blank">Engineering BioBrick vectors from BioBrick parts</a><br />
Shetty (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Identification of the mob Genes of Plasmid pSC101 and Characterization of a Hybrid pSC101-R1162 System for Conjugal Mobilization" target="_blank">Identification of the mob Genes of Plasmid pSC101 and Characterization of a Hybrid pSC101-R1162 System for Conjugal Mobilization</a><br />
Meyer (2000)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Primary structure of the essential replicon of the plasmid pSC101" target="_blank">Primary structure of the essential replicon of the plasmid pSC101</a><br />
Vocke (1983)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Replication of plasmid pSC101" target="_blank">Replication of plasmid pSC101</a><br />
Manen (1991)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Simple and highly efficient BAC recombineering using galK selection" target="_blank">Simple and highly efficient BAC recombineering using galK selection</a><br />
Warming (2005) <br />
</p><br />
<br />
<br />
<!-- <div align="justify" style="padding-left:20px; padding-right:20px"> --><br />
<div align="justify"><br />
<br />
<br />
</div><br />
</div></div><br />
<b class="b4f"></b><b class="b3f"></b><b class="b2f"></b><b class="b1f"></b><br />
</td><br />
</tr><br />
</table><br />
</div><br />
</HTML></div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Keyword
Team:Alberta/References/Keyword
2009-10-20T07:32:08Z
<p>Wiltshir: </p>
<hr />
<div>{{:Team:Alberta/Template3}}<br />
<html><br />
<head><br />
<style type="text/css"><br />
.b1f, .b2f, .b3f, .b4f{font-size:1px; overflow:hidden; display:block;}<br />
.b1f {height:1px; background:#e1e1e1; margin:0 5px;}<br />
.b2f {height:1px; background:#e1e1e1; margin:0 3px;}<br />
.b3f {height:1px; background:#e1e1e1; margin:0 2px;}<br />
.b4f {height:2px; background:#e1e1e1; margin:0 1px;}<br />
.content {background: #e1e1e1;}<br />
.content div {margin-left: 5px;}<br />
</style><br />
</head><br />
<br />
<div class="all"><br />
<div style="background:#FFFFFF"><br />
<br />
<!-- adjust table width, main background and padding between cells and edge of background --><br />
<br />
<br />
<table width=60% style="background:#FFFFFF; padding:2px;"><br />
<br />
<tr><br />
<td style="height: 400; padding-left: 10px; padding-right: 10px; padding-top: 11px;"><br />
<b class="b1f"></b><b class="b2f"></b><b class="b3f"></b><b class="b4f"></b><br />
<div class="Recoli"><br />
<div style="height: 400; background:#FFFFFF; colorou line-height:100% padding: 3px 0px;"><br />
<h1>References</h1><br />
<br />
<h2>Bioinformatics</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/BioWarehouse: a bioinformatics database warehouse toolkit" target="_blank">BioWarehouse: a bioinformatics database warehouse toolkit</a><br />
Lee (2006)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Triad pattern algorithms for predicting strong promoter candidates in bacterial genomes">Triad pattern algorithms for predicting strong promoter candidates in bacterial genomes</a><br />
Dekhtyar (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Vector NTI, a balanced all-in-one sequence analysis suite">Vector NTI, a balanced all-in-one sequence analysis suite</a><br />
Lu (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/Project/Modeling">E. Coli Metabolic Modelling in Matlab</a><br />
</p><br />
<br />
<h2>Deletion</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda red">An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda red</a><br />
Fukiya (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome">Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome</a><br />
Hashimoto (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Construction of consecutive deletions of the Escherichia coli chromosome">Construction of consecutive deletions of the Escherichia coli chromosome</a><br />
Kato (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Construction of Escherichia coli K-12 in-frame, single gene knockout mutants: the Keio collection">Construction of Escherichia coli K-12 in-frame, single gene knockout mutants: the Keio collection</a><br />
Baba (2006)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome">Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome</a><br />
Kato (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Emergent properties of reduced genome Escherichia coli">Emergent properties of reduced genome Escherichia coli</a><br />
Posfai (2006)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Engineering a reduced Escherichia coli genome">Engineering a reduced Escherichia coli genome</a><br />
Kolisnychenko (2002)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Rapid and efficient construction of markerless deletions in the Escherichia coli genome">Rapid and efficient construction of markerless deletions in the Escherichia coli genome</a><br />
Yu (2008) <br />
</p><br />
<br />
<h2>DNA Assembly</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium">Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium</a><br />
Gibson (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine">DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine</a><br />
Kelman (1995)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/DNA Replication">DNA Replication</a><br />
Kornberg (1988)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Enzymatic assembly of DNA molecules up to several hundred kilobases">Enzymatic assembly of DNA molecules up to several hundred kilobases</a><br />
Gibson (2009)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Rebuilding microbial genomes">Rebuilding microbial genomes</a><br />
Holt (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Refactoring bacteriophage T7">Refactoring bacteriophage T7</a><br />
Chan (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/The replication-related organization of bacterial genomes">The replication-related organization of bacterial genomes</a><br />
Rocha (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Shear induced assembly of lambda phage DNA">Shear induced assembly of lambda phage DNA</a><br />
Haber (2000)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Structural basis for uracil recognition by archaeal family B DNA polymerases">Structural basis for uracil recognition by archaeal family B DNA polymerases</a><br />
Fogg (2002)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/USERTM friendly DNA engineering and cloning method by uracil excision">USERTM friendly DNA engineering and cloning method by uracil excision</a><br />
Bitinaite (2007) <br />
</p><br />
<br />
<h2>E. coli Physiology</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome">Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome</a><br />
Hashimoto (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Escherichia coli physiology in Luria-Bertani broth">Escherichia coli physiology in Luria-Bertani broth</a><br />
Sezonov (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Fermentation pathways of Escherichia coli, The">Fermentation pathways of Escherichia coli, The</a><br />
Clark (1989)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110">Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110</a><br />
Hayashi (2006)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/The replication-related organization of bacterial genomes">The replication-related organization of bacterial genomes</a><br />
Rocha (2004) <br />
</p><br />
<br />
<h2>Essential Genes</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/The adjacent dnaZ and dnaX genes of Escherichia coli are contained within one continuous open reading frame">The adjacent dnaZ and dnaX genes of Escherichia coli are contained within one continuous open reading frame</a><br />
Flower (1986)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Chromosomal Locations of the Genes for rRNA in Escherichia coli K-12t">Chromosomal Locations of the Genes for rRNA in Escherichia coli K-12t</a><br />
Ellwood (1982)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Construction of Escherichia coli K-12 in-frame, single gene knockout mutants: the Keio collection">Construction of Escherichia coli K-12 in-frame, single gene knockout mutants: the Keio collection</a><br />
Baba (2006)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Complete set of ORF clones of Escherichia coli ASKA library (a complete set of Escherichia coli K-12 ORF archive): unique resources for biological research">Complete set of ORF clones of Escherichia coli ASKA library (a complete set of Escherichia coli K-12 ORF archive): unique resources for biological research</a><br />
Kitagawa (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Determination of the core of a minimal bacterial gene set">Determination of the core of a minimal bacterial gene set</a><br />
Gil (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine">DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine</a><br />
Kelman (1995)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Experimental determination and systems level analysis of essential genes in Escherichia coli MG1665">Experimental determination and systems level analysis of essential genes in Escherichia coli MG1665</a><br />
Gerdes (2003)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/The Heat-Shock-Regulated grpE Gene of Escherichia coli Is Required for Bacterial Growth at All Temperatures but Is Dispensable in Certain Mutant Backgrounds">The Heat-Shock-Regulated grpE Gene of Escherichia coli Is Required for Bacterial Growth at All Temperatures but Is Dispensable in Certain Mutant Backgrounds</a><br />
Ang (1989)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Operon Structure of dnaT and dnaC Genes Essential for Normal and Stable DNA Replication of Escherichia coli Chromosome">Operon Structure of dnaT and dnaC Genes Essential for Normal and Stable DNA Replication of Escherichia coli Chromosome</a><br />
Masai (1988)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Site-directed Mutational Analysis for the ATP Binding of DnaA Protein">Site-directed Mutational Analysis for the ATP Binding of DnaA Protein</a><br />
Mizushima (1998) <br />
</p><br />
<br />
<h2>Genes, Other</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Coding-sequence determinants of gene expression in Escherichia coli">Coding-sequence determinants of gene expression in Escherichia coli</a><br />
Kudla (2009)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Compilation and analysis of Escherichia coli promoter DNA sequences">Compilation and analysis of Escherichia coli promoter DNA sequences</a><br />
Hawley (1983)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Effect of dna Mutations on the Replication of Plasmid pSC101 in Escherichia coli K-12">Effect of dna Mutations on the Replication of Plasmid pSC101 in Escherichia coli K-12</a><br />
Hasunuma (1979)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Genome-wide expression profiling in Escherichia coli K-12">Genome-wide expression profiling in Escherichia coli K-12</a><br />
Richmond (1999)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110">Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110</a><br />
Hayashi (2006)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Idempotent Vector Design for Standard Assembly of Biobricks">Idempotent Vector Design for Standard Assembly of Biobricks</a><br />
Knight 2003<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli">Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli</a><br />
Morgan (1977)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Programming gene expression with combinatorial promoters">Programming gene expression with combinatorial promoters</a><br />
Cox (2007) <br />
</p><br />
<br />
<h2>Microfluidics</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/An inexpensive and portable microchip-based platform for integrated<br />
RT–PCR and capillary electrophoresis">An inexpensive and portable microchip-based platform for integrated<br />
RT–PCR and capillary electrophoresis</a><br />
Kaigala (2008)<br />
</p><br />
<br />
<h2>Nucleic Acid Properties</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Binding of biotinylated DNA to streptavidin-coated polystyrene latex: effects of chain length and particle size">Binding of biotinylated DNA to streptavidin-coated polystyrene latex: effects of chain length and particle size</a><br />
Huang (1996)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Dynamic strength of molecular adhesion bonds">Dynamic strength of molecular adhesion bonds</a><br />
Evans (1997)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/The effect of hydrodynamic shear of DNA from T2 and T4 bacteriophages">The effect of hydrodynamic shear of DNA from T2 and T4 bacteriophages</a><br />
Davison (1959)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Effect of shear on plasmid DNA in solution">Effect of shear on plasmid DNA in solution</a><br />
Levy (1999)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Folding and unfolding single RNA molecules under tension">Folding and unfolding single RNA molecules under tension</a><br />
Woodside (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays">Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays</a><br />
Bernstein (2002)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Shear breakage of DNA">Shear breakage of DNA</a><br />
Dancis (1978)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Shear degradation of DNA">Shear degradation of DNA</a><br />
Adam (1977)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Shear-induced degradation of plasmid DNA">Shear-induced degradation of plasmid DNA</a><br />
Lengsfeld (2002)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Shear unzipping of DNA">Shear unzipping of DNA</a><br />
Chakrabarti (2009)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Stochastic simulations of DNA in flow: dynamics and the effects of hydrodynamic interactions">Stochastic simulations of DNA in flow: dynamics and the effects of hydrodynamic interactions</a><br />
Jendrejack (2002) <br />
</p><br />
<br />
<h2>Operons</h2><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Engineering of bacterial ribosomes: Replacement of all seven Escherichia coli rRNA operons by a single plasmid-encoded operon">Engineering of bacterial ribosomes: Replacement of all seven Escherichia coli rRNA operons by a single plasmid-encoded operon</a><br />
Nomura (1999)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli">Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli</a><br />
Morgan (1977)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Operon Structure of dnaT and dnaC Genes Essential for Normal and Stable DNA Replication of Escherichia coli Chromosome">Operon Structure of dnaT and dnaC Genes Essential for Normal and Stable DNA Replication of Escherichia coli Chromosome</a><br />
Masai (1988) <br />
</p><br />
<br />
<h2>Recombination</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda red">An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda red</a><br />
Fukiya (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Biochemistry of homologous recombination in Escherichia coli">Biochemistry of homologous recombination in Escherichia coli</a><br />
Kowalczykowski (1994)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome">Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome</a><br />
Hashimoto (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector">Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector</a><br />
Shizuya (1992)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Conditional-Replication, Integration, Excision, and Retrieval Plasmid-Host Systems for Gene Structure-Function Studies of Bacteria">Conditional-Replication, Integration, Excision, and Retrieval Plasmid-Host Systems for Gene Structure-Function Studies of Bacteria</a><br />
Haldimann (2001)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Construction of consecutive deletions of the Escherichia coli chromosome">Construction of consecutive deletions of the Escherichia coli chromosome</a><br />
Kato (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome">Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome</a><br />
Kato (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/DNA cloning by homologous recombination in Escherichia coli">DNA cloning by homologous recombination in Escherichia coli</a><br />
Zhang (2000)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Engineering a reduced Escherichia coli genome">Engineering a reduced Escherichia coli genome</a><br />
Kolisnychenko (2002)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Gene replacement without selection: regulated suppression of amber mutations in Escherichia coli">Gene replacement without selection: regulated suppression of amber mutations in Escherichia coli</a><br />
Herring (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Gene splicing and mutagenesis by PCR driven overlap extension">Gene splicing and mutagenesis by PCR driven overlap extension</a><br />
Heckman (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/In vivo transposition of mariner based elements in enteric bacteria and mycobacteria">In vivo transposition of mariner based elements in enteric bacteria and mycobacteria</a><br />
Rubin (1999)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Markerless gene replacement in Escherichia coli stimulated by double strand break in the chromosome">Markerless gene replacement in Escherichia coli stimulated by double strand break in the chromosome</a><br />
Posfai (1999)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/One step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome">One step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome</a><br />
Gibson (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products">One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products</a><br />
Datsenko (2000)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Phage integrases: biology and applications">Phage integrases: biology and applications</a><br />
Groth (2004)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Rapid and efficient construction of markerless deletions in the Escherichia coli genome">Rapid and efficient construction of markerless deletions in the Escherichia coli genome</a><br />
Yu (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Recombineering with tolC as a selectable/counter-selectable marker: remodeling the rRNA operons in Escherichia coli">Recombineering with tolC as a selectable/counter-selectable marker: remodeling the rRNA operons in Escherichia coli</a><br />
DeVito (2007)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Scarless engineering of the Escherichia coli genome">Scarless engineering of the Escherichia coli genome</a><br />
Feher (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Shear induced assembly of lambda phage DNA">Shear induced assembly of lambda phage DNA</a><br />
Haber (2000)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Simple and highly efficient BAC recombineering using galK selection">Simple and highly efficient BAC recombineering using galK selection</a><br />
Warming (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Techniques: recombinogenic engineering - new options for cloning and manipulating DNA">Techniques: recombinogenic engineering - new options for cloning and manipulating DNA</a><br />
Muyrers (2001)<br />
</p><br />
<br />
<h2>Synthetic Biology</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Engineering BioBrick vectors from BioBrick parts">Engineering BioBrick vectors from BioBrick parts</a><br />
Shetty (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Idempotent Vector Design for Standard Assembly of Biobricks">Idempotent Vector Design for Standard Assembly of Biobricks</a><br />
Knight 2003<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Refactoring bacteriophage T7">Refactoring bacteriophage T7</a><br />
Chan (2005)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Refinement and standardization of synthetic biological parts and devices">Refinement and standardization of synthetic biological parts and devices</a><br />
Canton (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Setting the standard in synthetic biology">Setting the standard in synthetic biology</a><br />
Arkin (2008) <br />
</p><br />
<br />
<h2>Transformation</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Transformation of Escherichia coli with large DNA molecules by electroporation">Transformation of Escherichia coli with large DNA molecules by electroporation</a><br />
Sheng (1995) <br />
</p><br />
<br />
<h2>Vectors</h2><br />
<br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/A family of LIC vectors for high through-put cloning and purification of proteins">A family of LIC vectors for high through-put cloning and purification of proteins</a><br />
Eschenfeldt (2009)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector">Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector</a><br />
Shizuya (1992)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Effect of dna Mutations on the Replication of Plasmid pSC101 in Escherichia coli K-12">Effect of dna Mutations on the Replication of Plasmid pSC101 in Escherichia coli K-12</a><br />
Hasunuma (1979)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Engineering BioBrick vectors from BioBrick parts">Engineering BioBrick vectors from BioBrick parts</a><br />
Shetty (2008)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Identification of the mob Genes of Plasmid pSC101 and Characterization of a Hybrid pSC101-R1162 System for Conjugal Mobilization">Identification of the mob Genes of Plasmid pSC101 and Characterization of a Hybrid pSC101-R1162 System for Conjugal Mobilization</a><br />
Meyer (2000)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Primary structure of the essential replicon of the plasmid pSC101">Primary structure of the essential replicon of the plasmid pSC101</a><br />
Vocke (1983)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Replication of plasmid pSC101">Replication of plasmid pSC101</a><br />
Manen (1991)<br />
</p><br />
<p><br />
<a href="https://2009.igem.org/Team:Alberta/References/Publications/Simple and highly efficient BAC recombineering using galK selection">Simple and highly efficient BAC recombineering using galK selection</a><br />
Warming (2005) <br />
</p><br />
<br />
<br />
<!-- <div align="justify" style="padding-left:20px; padding-right:20px"> --><br />
<div align="justify"><br />
<br />
<br />
</div><br />
</div></div><br />
<b class="b4f"></b><b class="b3f"></b><b class="b2f"></b><b class="b1f"></b><br />
</td><br />
</tr><br />
</table><br />
</div><br />
</HTML></div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Construction_of_consecutive_deletions_of_the_Escherichia_coli_chromosome
Team:Alberta/References/Publications/Construction of consecutive deletions of the Escherichia coli chromosome
2009-10-20T07:30:43Z
<p>Wiltshir: </p>
<hr />
<div>Jun-ichi Kato and Masayuki Hashimoto<br />
<br />
Mol Syst Biol. 2007;3:132. Epub 2007 Aug 14<br />
<br />
<br />
<b>Abstract:</b> The minimal set of genetic information necessary and sufficient to sustain a functioning cell contains not only trans-acting genes, but also cis-acting chromosomal regions that cannot be complemented by plasmids carrying these regions. In Escherichia coli (E. coli), only one chromosomal region, the origin of replication has been identified to be cis-acting. We constructed a series of mutants with long-range deletions, and the chromosomal regions containing trans-acting essential genes were deleted in the presence of plasmids complementing the deleted genes. The deleted regions cover all regions of the chromosome except for the origin and terminus of replication. The terminus affects cell growth, but is not essential. Our results indicate that the origin of DNA replication is the only vital, unique cis-acting DNA sequence in the E. coli chromosome necessary for survival.<br />
<br />
<br />
'''Link:''' [http://www.ncbi.nlm.nih.gov/pubmed/17700540 Pubmed]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Construction_of_consecutive_deletions_of_the_Escherichia_coli_chromosome
Team:Alberta/References/Publications/Construction of consecutive deletions of the Escherichia coli chromosome
2009-10-20T07:30:17Z
<p>Wiltshir: </p>
<hr />
<div><html><br />
<title>University of Alberta - BioBytes</title><br />
<img src="https://static.igem.org/mediawiki/2009/0/04/UofA_biobytes_HeaderBanner5.png" width="965" height="235"><br />
</html><br />
<br><br><br />
<br />
Jun-ichi Kato and Masayuki Hashimoto<br />
<br />
Mol Syst Biol. 2007;3:132. Epub 2007 Aug 14<br />
<br />
<br />
<b>Abstract:</b> The minimal set of genetic information necessary and sufficient to sustain a functioning cell contains not only trans-acting genes, but also cis-acting chromosomal regions that cannot be complemented by plasmids carrying these regions. In Escherichia coli (E. coli), only one chromosomal region, the origin of replication has been identified to be cis-acting. We constructed a series of mutants with long-range deletions, and the chromosomal regions containing trans-acting essential genes were deleted in the presence of plasmids complementing the deleted genes. The deleted regions cover all regions of the chromosome except for the origin and terminus of replication. The terminus affects cell growth, but is not essential. Our results indicate that the origin of DNA replication is the only vital, unique cis-acting DNA sequence in the E. coli chromosome necessary for survival.<br />
<br />
<br />
'''Link:''' [http://www.ncbi.nlm.nih.gov/pubmed/17700540 Pubmed]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/An_inexpensive_and_portable_microchip-based_platform_for_integratedRT%E2%80%93PCR_and_capillary_electrophoresis
Team:Alberta/References/Publications/An inexpensive and portable microchip-based platform for integratedRT–PCR and capillary electrophoresis
2009-10-20T07:25:42Z
<p>Wiltshir: New page: Authors Journal #### MONTH;##(#):PAGE-PAGE <b>Abstract:</b> ---- '''Link:''' [http://eeeeeeeeeeeee NAME]</p>
<hr />
<div>Authors<br />
<br />
Journal #### MONTH;##(#):PAGE-PAGE<br />
<br />
<br />
<b>Abstract:</b> ----<br />
<br />
<br />
<br />
'''Link:''' [http://eeeeeeeeeeeee NAME]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Replication_of_plasmid_pSC101
Team:Alberta/References/Publications/Replication of plasmid pSC101
2009-10-20T07:23:19Z
<p>Wiltshir: New page: D. Manen and L. Caro Mol Microbiol. 1991 Feb;5(2):233-7 <b>Abstract:</b> The origin of replication of plasmid pSC101 presents features reminiscent of those found in a number of plasmids...</p>
<hr />
<div>D. Manen and L. Caro<br />
<br />
Mol Microbiol. 1991 Feb;5(2):233-7<br />
<br />
<br />
<b>Abstract:</b> The origin of replication of plasmid pSC101 presents features reminiscent of those found in a number of plasmids. As for those plasmids, many details about the way it initiates its replication are beginning to be known, but the regulation of this process will not be easily understood.<br />
<br />
<br />
<br />
<b>Link:</b> [http://www.ncbi.nlm.nih.gov/pubmed/2041467?ordinalpos=3&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum NCBI]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Primary_structure_of_the_essential_replicon_of_the_plasmid_pSC101
Team:Alberta/References/Publications/Primary structure of the essential replicon of the plasmid pSC101
2009-10-20T07:22:51Z
<p>Wiltshir: New page: C. Vocke and D. Bastia Proc Natl Acad Sci U S A. 1983 November; 80(21): 6557–6561 <b>Abstract:</b> The replicon of the low copy number plasmid pSC101 has an obligatory requirement for...</p>
<hr />
<div>C. Vocke and D. Bastia<br />
<br />
Proc Natl Acad Sci U S A. 1983 November; 80(21): 6557–6561<br />
<br />
<br />
<b>Abstract:</b> The replicon of the low copy number plasmid pSC101 has an obligatory requirement for the dnaA initiator protein of Escherichia coli as well as a plasmid-encoded initiator protein. We have identified the cistron of the plasmid-encoded initiator by DNA sequence analysis. Fusion of the initiator cistron with the lacZ gene of E. coli yielded a fusion protein of approximately equal to 150 kilodaltons, thus confirming that the open reading frame detected by DNA sequence analysis actually encoded a 37.5-kilodalton protein. Deletion of 26 amino acid residues from the COOH terminus of the plasmid initiator abolished autonomous replication from pSC101 origin. By in vitro deletion analysis we have shown that, although sequences downstream from the initiator cistron are dispensable, a maximum of 400 base pairs immediately upstream from the NH2-terminal region of the initiator is necessary for plasmid replication. These upstream sequences contain an A + T-rich region and three tandem repeats of a 21-base pair sequence; these features are characteristics of other replication origins.<br />
<br />
<br />
<br />
<b>Link:</b> [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=390392&tool=pmcentrez PubMed]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Identification_of_the_mob_Genes_of_Plasmid_pSC101_and_Characterization_of_a_Hybrid_pSC101-R1162_System_for_Conjugal_Mobilization
Team:Alberta/References/Publications/Identification of the mob Genes of Plasmid pSC101 and Characterization of a Hybrid pSC101-R1162 System for Conjugal Mobilization
2009-10-20T07:22:27Z
<p>Wiltshir: New page: Richard Meyer J Bacteriol. 2000 September; 182(17): 4875–4881 <b>Abstract:</b> Similarities in DNA base sequence indicate that pSC101 and R1162 encode related systems for conjugal mob...</p>
<hr />
<div>Richard Meyer<br />
<br />
J Bacteriol. 2000 September; 182(17): 4875–4881<br />
<br />
<br />
<b>Abstract:</b> Similarities in DNA base sequence indicate that pSC101 and R1162 encode related systems for conjugal mobilization, although these plasmids are otherwise very different. The mob region of pSC101 was cloned, and two genes that are required for transfer were identified. One gene, mobA, encodes a protein similar in amino acid sequence to the DNA processing domain of the R1162 MobA protein. The other gene, mobX, is within the same transcriptional unit as the pSC101 mobA and is located just downstream, at the same position occupied by mobB in R1162. Despite this, the MobB and MobX proteins do not appear to be closely related based on a comparison of their amino acid sequences. Complementation analysis indicated that neither of the pSC101 Mob proteins could substitute for, or be replaced by, their R1162 counterparts, nor were they active together at the R1162 origin of transfer (oriT). However, the full set of R1162 Mob proteins did recognize the pSC101 oriT. A hybrid system for mobilization, active at the R1162 oriT site, was constructed. This system consists of MobX and a chimeric protein made up of the DNA cleaving-ligating domain of the R1162 MobA protein joined to a fragment of pSC101 MobA. Previous results suggested that MobB and a region of MobA distinct from the DNA processing domain together formed a functional unit in transfer. The present results support this model because the chimeric MobA, although active on R1162 oriT, requires the pSC101 protein MobX for efficient plasmid mobilization.<br />
<br />
<br />
<br />
<b>Link:</b> [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=111367&tool=pmcentrez&rendertype=abstract PubMed]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/A_family_of_LIC_vectors_for_high_through-put_cloning_and_purification_of_proteins
Team:Alberta/References/Publications/A family of LIC vectors for high through-put cloning and purification of proteins
2009-10-20T07:21:44Z
<p>Wiltshir: New page: William H. Eschenfeldt, Lucy Stols, Cynthia Sanville Millard, Andrzej Joachimiak and Mark I. Donnelly from: Sharon A. Doyle (ed.), Methods in Molecular Biology: High Throughput Protein Ex...</p>
<hr />
<div>William H. Eschenfeldt, Lucy Stols, Cynthia Sanville Millard, Andrzej Joachimiak and Mark I. Donnelly<br />
<br />
from: Sharon A. Doyle (ed.), Methods in Molecular Biology: High Throughput Protein Expression and Purification, vol. 498<br />
© 2009 Humana Press, a part of Springer Science + Business Media, Totowa, NJ<br />
<br />
<b>Abstract:</b> Fifteen related ligation-independent cloning vectors were constructed for high-throughput cloning and purification of proteins. The vectors encode a TEV protease site for removal of tags that facilitate protein purification (his-tag) or improve solubility (MBP, GST). Specialized vectors allow coexpression and copurification of interacting proteins, or in vivo removal of MBP by TVMV protease to improve screening and purification. All target genes and vectors are processed by the same protocols, which we<br />
describe here.<br />
<br />
<br />
<br />
'''Link''' [http://www.springerlink.com/content/hr80631721158226/fulltext.pdf Springerlink]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Transformation_of_Escherichia_coli_with_large_DNA_molecules_by_electroporation
Team:Alberta/References/Publications/Transformation of Escherichia coli with large DNA molecules by electroporation
2009-10-20T07:21:14Z
<p>Wiltshir: New page: YuLing Sheng, Valeria Mancino and Bruce Birren Nucleic Acids Res. 1995 June 11; 23(11): 1990–1996 '''Abstract:''' We have examined bacterial electroporation with a specific Interest i...</p>
<hr />
<div>YuLing Sheng, Valeria Mancino and Bruce Birren<br />
<br />
Nucleic Acids Res. 1995 June 11; 23(11): 1990–1996<br />
<br />
<br />
'''Abstract:''' We have examined bacterial electroporation with a specific Interest in the transformation of large DNA, I.e.<br />
molecules >100 kb. We have used DNA from bacterial artificial chromosomes (BACs) ranging from 7 to 240 kb, as well as BAC ligatlon mixes containing a range of different sized molecules. The efficiency of electroporation with large DNA is strongly dependent on the<br />
strain of ''Escherichia coli'' used; strains which offer comparable efficiencies for 7 kb molecules differ In their uptake of 240 kb DNA by as much as 30-fold. Even with a host strain that transforms relatively well with large DNA, transformation efficiency drops dramatically with Increasing size of the DNA. Molecules of 240 kb transform ~30-fold less well, on a molar basis, than molecules of 80 kb. Maximum transformation of large DNA occurs with different voltage gradients and with different time constants than are optimal for smaller DNA. This provides the opportunity to Increase the yield of transformants which have taken up large DNA relative to the number incorporating smaller molecules. We have demonstrated that conditions may be selected which increase the average size of BAC<br />
clones generated by electroporation and compare the overall efficiency of each of the conditions tested.<br />
<br />
<br />
<br />
'''Link:''' [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=306974&tool=pmcentrez PubMed]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Setting_the_standard_in_synthetic_biology
Team:Alberta/References/Publications/Setting the standard in synthetic biology
2009-10-20T07:20:42Z
<p>Wiltshir: New page: Adam Arkin Nature Biotechnology 26, 771 - 774 (2008) <b>Abstract:</b> Standards for characterization, manufacture and sharing of information about modular biological devices may lead to...</p>
<hr />
<div>Adam Arkin<br />
<br />
Nature Biotechnology 26, 771 - 774 (2008)<br />
<br />
<br />
<b>Abstract:</b> Standards for characterization, manufacture and sharing of information about modular biological devices may lead<br />
to a more efficient, predictable and design-driven genetic engineering science.<br />
<br />
<br />
'''Link:''' [http://www.nature.com/nbt/journal/v26/n7/full/nbt0708-771.html Nature]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Refinement_and_standardization_of_synthetic_biological_parts_and_devices
Team:Alberta/References/Publications/Refinement and standardization of synthetic biological parts and devices
2009-10-20T07:20:20Z
<p>Wiltshir: New page: Barry Canton, Anna Labno and Drew Endy Refinement and standardization of synthetic biological parts and devices <b>Abstract:</b> The ability to quickly and reliably engineer many-compon...</p>
<hr />
<div>Barry Canton, Anna Labno and Drew Endy<br />
<br />
Refinement and standardization of synthetic biological parts and devices<br />
<br />
<br />
<b>Abstract:</b> The ability to quickly and reliably engineer many-component systems from libraries of standard interchangeable parts is one hallmark of modern technologies. Whether the apparent complexity of living systems will permit biological engineers to<br />
develop similar capabilities is a pressing research question. We propose to adapt existing frameworks for describing engineered<br />
devices to biological objects in order to (i) direct the refinement and use of biological ‘parts’ and ‘devices’, (ii) support research<br />
on enabling reliable composition of standard biological parts and (iii) facilitate the development of abstraction hierarchies<br />
that simplify biological engineering. We use the resulting framework to describe one engineered biological device, a<br />
genetically encoded cell-cell communication receiver named BBa_F2620. The description of the receiver is summarized<br />
via a ‘datasheet’ similar to those widely used in engineering. The process of refinement and characterization leading to the<br />
BBa_F2620 datasheet may serve as a starting template for producing many standardized genetically encoded objects.<br />
<br />
<br />
'''Link:''' [http://www.nature.com/nbt/journal/v26/n7/abs/nbt1413.html Nature]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Engineering_BioBrick_vectors_from_BioBrick_parts
Team:Alberta/References/Publications/Engineering BioBrick vectors from BioBrick parts
2009-10-20T07:19:45Z
<p>Wiltshir: New page: Reshma P Shetty, Drew Endy and Thomas F Knight Jr Journal of Biological Engineering 2008, 2:5 '''Abstract:''' Background: The underlying goal of synthetic biology is to make the proce...</p>
<hr />
<div>Reshma P Shetty, Drew Endy and Thomas F Knight Jr<br />
<br />
Journal of Biological Engineering 2008, 2:5<br />
<br />
<br />
'''Abstract:''' <br />
<br />
Background: The underlying goal of synthetic biology is to make the process of engineering<br />
biological systems easier. Recent work has focused on defining and developing standard biological<br />
parts. The technical standard that has gained the most traction in the synthetic biology community<br />
is the BioBrick standard for physical composition of genetic parts. Parts that conform to the<br />
BioBrick assembly standard are BioBrick standard biological parts. To date, over 2,000 BioBrick<br />
parts have been contributed to, and are available from, the Registry of Standard Biological Parts.<br />
<br />
Results: Here we extended the same advantages of BioBrick standard biological parts to the<br />
plasmid-based vectors that are used to provide and propagate BioBrick parts. We developed a<br />
process for engineering BioBrick vectors from BioBrick parts. We designed a new set of BioBrick<br />
parts that encode many useful vector functions. We combined the new parts to make a BioBrick<br />
base vector that facilitates BioBrick vector construction. We demonstrated the utility of the<br />
process by constructing seven new BioBrick vectors. We also successfully used the resulting<br />
vectors to assemble and propagate other BioBrick standard biological parts.<br />
<br />
Conclusion: We extended the principles of part reuse and standardization to BioBrick vectors.<br />
As a result, myriad new BioBrick vectors can be readily produced from all existing and newly<br />
designed BioBrick parts. We invite the synthetic biology community to (1) use the process to make<br />
and share new BioBrick vectors; (2) expand the current collection of BioBrick vector parts; and (3)<br />
characterize and improve the available collection of BioBrick vector parts.<br />
<br />
<br />
<br />
'''Link:''' [http://www.jbioleng.org/content/2/1/5 Journal of Biological Engineering]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Techniques:_recombinogenic_engineering_-_new_options_for_cloning_and_manipulating_DNA
Team:Alberta/References/Publications/Techniques: recombinogenic engineering - new options for cloning and manipulating DNA
2009-10-20T07:19:11Z
<p>Wiltshir: New page: Joep P.P. Muyrers, Youming Zhang and A. Francis Stewart Trends Biochem Sci. 2001 May;26(5):325-31 '''Abstract:''' Driven by the needs of functional genomics, DNA engineering by homologo...</p>
<hr />
<div>Joep P.P. Muyrers, Youming Zhang and A. Francis Stewart<br />
<br />
Trends Biochem Sci. 2001 May;26(5):325-31<br />
<br />
<br />
'''Abstract:''' Driven by the needs of functional genomics, DNA engineering by homologous<br />
recombination in Escherichia coli has emerged as a major addition to existing<br />
technologies. Two alternative approaches, RecA-dependent engineering and<br />
ET recombination, allow a wide variety of DNA modifications, including some<br />
which are virtually impossible by conventional methods. These approaches do<br />
not rely on the presence of suitable restriction sites and can be used to insert,<br />
delete or substitute DNA sequences at any desired position on a target<br />
molecule. Furthermore, ET recombination can be used for direct subcloning<br />
and cloning of DNA sequences from complex mixtures, including bacterial<br />
artificial chromosomes and genomic DNA preparations. The strategies<br />
reviewed in this article are applicable to modification of DNA molecules of any<br />
size, including very large ones, and present powerful new avenues for DNA<br />
manipulation in general.<br />
<br />
<br />
<br />
'''Link:''' [http://www.ncbi.nlm.nih.gov/pubmed/11343926 NCBI]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Simple_and_highly_efficient_BAC_recombineering_using_galK_selection
Team:Alberta/References/Publications/Simple and highly efficient BAC recombineering using galK selection
2009-10-20T07:18:44Z
<p>Wiltshir: New page: Soren Warming, Nina Costantino, Donald L. Court, Nancy A. Jenkins and Neal G. Copeland Nucleic Acids Research, 2005, Vol. 33, No. 4 e36 '''Abstract:''' Recombineering allows DNA cloned ...</p>
<hr />
<div>Soren Warming, Nina Costantino, Donald L. Court, Nancy A. Jenkins and Neal G. Copeland<br />
<br />
Nucleic Acids Research, 2005, Vol. 33, No. 4 e36<br />
<br />
<br />
'''Abstract:''' Recombineering allows DNA cloned in ''Escherichia<br />
coli'' to be modified via lambda Red-mediated<br />
homologous recombination, obviating the need for<br />
restriction enzymes and DNA ligases to modify DNA.<br />
Here, we describe the construction of three new<br />
recombineering strains (SW102, SW105 and SW106)<br />
that allow bacterial artificial chromosomes (BACs) to<br />
be modified using ''galK'' positive/negative selection.<br />
This two-step selection procedure allows DNA to be<br />
modified without introducing an unwanted selectable<br />
marker at the modification site. All three strains contain<br />
an otherwise complete galactose operon, except<br />
for a precise deletion of the ''galK'' gene, and a defective<br />
temperature-sensitive lambda prophage that makes<br />
recombineering possible. SW105 and SW106 cells<br />
in addition carry L-arabinose-inducible ''Cre'' or ''Flp''<br />
genes, respectively. The ''galK'' function can be selected<br />
both for and against. This feature greatly reduces<br />
the background seen in other negative-selection<br />
schemes, and ''galK'' selection is considerably more<br />
efficient than other related selection methods<br />
published. We also show how ''galK'' selection can be<br />
used to rapidly introduce point mutations, deletions<br />
and ''loxP'' sites into BAC DNA and thus facilitate<br />
functional studies of SNP and/or disease-causing<br />
point mutations, the identification of long-range regulatory<br />
elements and the construction of conditional<br />
targeting vectors.<br />
<br />
<br />
'''Link:''' [http://nar.oxfordjournals.org/cgi/content/abstract/33/4/e36 Nucleic Acids Research]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Scarless_engineering_of_the_Escherichia_coli_genome
Team:Alberta/References/Publications/Scarless engineering of the Escherichia coli genome
2009-10-20T07:18:17Z
<p>Wiltshir: New page: Tamas Feher, Ildiko Karcagi, Zsuzsa Gyorfy, Kinga Umenhoffer, Balint Csorgo and Gyorgy Posfai Methods Mol Biol. 2008;416:251-9 '''Abstract:''' E. coli K-12, being one of the best unders...</p>
<hr />
<div>Tamas Feher, Ildiko Karcagi, Zsuzsa Gyorfy, Kinga Umenhoffer, Balint Csorgo and Gyorgy Posfai<br />
<br />
Methods Mol Biol. 2008;416:251-9<br />
<br />
<br />
'''Abstract:''' E. coli K-12, being one of the best understood and thoroughly analyzed organisms, is the workhorse<br />
of genetic, biochemical, and systems biology research, as well as the platform of choice for numerous<br />
biotechnological applications. Genome minimization/remodeling is now a feasible approach to further<br />
enhance its benefi cial characteristics for practical applications. Two genome engineering techniques, a<br />
lambda Red–mediated deletion method and a suicide (conditionally replicative) plasmid-based allele<br />
replacement procedure are presented here. These techniques utilize homologous recombination, and allow<br />
the rapid introduction of virtually any modifi cations in the genome.<br />
<br />
<br />
<br />
'''Link:''' [http://www.ncbi.nlm.nih.gov/pubmed/18392972 NCBI]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Recombineering_with_tolC_as_a_selectable/counter-selectable_marker:_remodeling_the_rRNA_operons_in_Escherichia_coli
Team:Alberta/References/Publications/Recombineering with tolC as a selectable/counter-selectable marker: remodeling the rRNA operons in Escherichia coli
2009-10-20T07:17:53Z
<p>Wiltshir: New page: Joseph A. DeVito Nucleic Acids Res. 2008 Jan;36(1):e4 <b>Abstract:</b> This work describes the novel use of tolC as a selectable/counter-selectable marker for the facile modification of...</p>
<hr />
<div>Joseph A. DeVito<br />
<br />
Nucleic Acids Res. 2008 Jan;36(1):e4<br />
<br />
<br />
<b>Abstract:</b> This work describes the novel use of tolC as a selectable/counter-selectable marker for the facile modification of DNA in Escherichia coli. Expression of TolC (an outer membrane protein) confers relative resistance to toxic small molecules, while its absence renders the cell tolerant to colicin E1. These features, coupled with the λredgam recombination system, allow for selection of tolC insertions/deletions anywhere on the E. coli chromosome or on plasmid DNA. This methodology obviates the need for minimal growth media, specialized wash protocols and the lengthy incubation times required by other published recombineering methods. As a rigorous test of the TolC selection system, six out of seven 23S rRNA genes were consecutively and seamlessly removed from the E. coli chromosome without affecting expression of neighboring genes within the complex rrn operons. The resulting plasmid-free strain retains one 23S rRNA gene (rrlC) in its natural location on the chromosome and is the first mutant of its kind. These new rRNA mutants will be useful in the study of rRNA gene regulation and ribosome function. Given its high efficiency, low background and facility in rich media, tolC selection is a broadly applicable method for the modification of DNA by recombineering.<br />
<br />
<br />
<br />
'''Link:''' [http://www.ncbi.nlm.nih.gov/pubmed/18084036 NCBI]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Phage_integrases:_biology_and_applications
Team:Alberta/References/Publications/Phage integrases: biology and applications
2009-10-20T07:17:21Z
<p>Wiltshir: New page: Amy C. Groth and Michele P. Calos J. Mol. Biol. (2004) 335, 667–678 '''Abstract:'''Phage integrases are enzymes that mediate unidirectional site-specific recombination between two DNA...</p>
<hr />
<div>Amy C. Groth and Michele P. Calos<br />
<br />
J. Mol. Biol. (2004) 335, 667–678<br />
<br />
<br />
'''Abstract:'''Phage integrases are enzymes that mediate unidirectional site-specific<br />
recombination between two DNA recognition sequences, the phage<br />
attachment site, attP, and the bacterial attachment site, attB. Integrases<br />
may be grouped into two major families, the tyrosine recombinases and<br />
the serine recombinases, based on their mode of catalysis. Tyrosine family<br />
integrases, such as lambda integrase, utilize a catalytic tyrosine to mediate<br />
strand cleavage, tend to recognize longer attP sequences, and require<br />
other proteins encoded by the phage or the host bacteria. Phage integrases<br />
from the serine family are larger, use a catalytic serine for strand cleavage,<br />
recognize shorter attP sequences, and do not require host cofactors. Phage<br />
integrases mediate efficient site-specific recombination between two<br />
different sequences that are relatively short, yet long enough to be specific<br />
on a genomic scale. These properties give phage integrases growing<br />
importance for the genetic manipulation of living eukaryotic cells,<br />
especially those with large genomes such as mammals and most plants,<br />
for which there are few tools for precise manipulation of the genome. Integrases<br />
of the serine family have been shown to work efficiently in mammalian<br />
cells, mediating efficient integration at introduced att sites or<br />
native sequences that have partial identity to att sites. This reaction has<br />
applications in areas such as gene therapy, construction of transgenic<br />
organisms, and manipulation of cell lines. Directed evolution can be<br />
used to increase further the affinity of an integrase for a particular native<br />
sequence, opening up additional applications for genomic modification.<br />
<br />
<br />
'''Link:''' [http://labs.fhcrc.org/stoddard/MCB_542/Integrases-Resolvases_JMB335p667.pdf JMB]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/One-step_inactivation_of_chromosomal_genes_in_Escherichia_coli_K-12_using_PCR_products
Team:Alberta/References/Publications/One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
2009-10-20T07:17:01Z
<p>Wiltshir: New page: Kirill A. Datsenko and Barry L. Wanner Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5 <b>Abstract:</b> We have developed a simple and highly efficient method to disrupt chromosomal ...</p>
<hr />
<div>Kirill A. Datsenko and Barry L. Wanner<br />
<br />
Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5<br />
<br />
<br />
<b>Abstract:</b> We have developed a simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s). In this procedure, recombination requires the phage λ Red recombinase, which is synthesized under the control of an inducible promoter on an easily curable, low copy number plasmid. To demonstrate the utility of this approach, we generated PCR products by using primers with 36- to 50-nt extensions that are homologous to regions adjacent to the gene to be inactivated and template plasmids carrying antibiotic resistance genes that are flanked by FRT (FLP recognition target) sites. By using the respective PCR products, we made 13 different disruptions of chromosomal genes. Mutants of the arcB, cyaA, lacZYA, ompR-envZ, phnR, pstB, pstCA, pstS, pstSCAB-phoU, recA, and torSTRCAD genes or operons were isolated as antibiotic-resistant colonies after the introduction into bacteria carrying a Red expression plasmid of synthetic (PCR-generated) DNA. The resistance genes were then eliminated by using a helper plasmid encoding the FLP recombinase which is also easily curable. This procedure should be widely useful, especially in genome analysis of E. coli and other bacteria because the procedure can be done in wild-type cells.<br />
<br />
<br />
<br />
'''Link:''' [http://www.ncbi.nlm.nih.gov/pubmed/10829079 NCBI]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Markerless_gene_replacement_in_Escherichia_coli_stimulated_by_double_strand_break_in_the_chromosome
Team:Alberta/References/Publications/Markerless gene replacement in Escherichia coli stimulated by double strand break in the chromosome
2009-10-20T07:16:03Z
<p>Wiltshir: New page: Gyorgy Posfai, Vitaliy Kolisnychenko, Zsuzsa Bereczki and Frederick R. Blattner Nucleic Acids Research, Vol 27, Issue 22 4409-4415, Copyright © 1999 by Oxford University Press '''Abst...</p>
<hr />
<div>Gyorgy Posfai, Vitaliy Kolisnychenko, Zsuzsa Bereczki and Frederick R. Blattner<br />
<br />
Nucleic Acids Research, Vol 27, Issue 22 4409-4415, Copyright © 1999 by Oxford University Press <br />
<br />
<br />
'''Abstract:''' Driven by the needs of functional genomics, DNA engineering by homologous<br />
recombination in Escherichia coli has emerged as a major addition to existing<br />
technologies. Two alternative approaches, RecA-dependent engineering and<br />
ET recombination, allow a wide variety of DNA modifications, including some<br />
which are virtually impossible by conventional methods. These approaches do<br />
not rely on the presence of suitable restriction sites and can be used to insert,<br />
delete or substitute DNA sequences at any desired position on a target<br />
molecule. Furthermore, ET recombination can be used for direct subcloning<br />
and cloning of DNA sequences from complex mixtures, including bacterial<br />
artificial chromosomes and genomic DNA preparations. The strategies<br />
reviewed in this article are applicable to modification of DNA molecules of any<br />
size, including very large ones, and present powerful new avenues for DNA<br />
manipulation in general.<br />
<br />
<br />
<br />
'''Link:''' [http://nar.oxfordjournals.org/cgi/content/abstract/27/22/4409 Nucleic Acids Research]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/In_vivo_transposition_of_mariner_based_elements_in_enteric_bacteria_and_mycobacteria
Team:Alberta/References/Publications/In vivo transposition of mariner based elements in enteric bacteria and mycobacteria
2009-10-20T07:15:09Z
<p>Wiltshir: New page: Eric J. Rubin, Brian J. Akerley, Veronica N. Novik, David J. Lampe, Robert N. Husson and John J. Mekalanos PNAS February 16, 1999 vol. 96 no. 4 1645-1650 '''Abstract:''' ''mariner'' fa...</p>
<hr />
<div>Eric J. Rubin, Brian J. Akerley, Veronica N. Novik, David J. Lampe, Robert N. Husson and John J. Mekalanos<br />
<br />
PNAS February 16, 1999 vol. 96 no. 4 1645-1650 <br />
<br />
<br />
'''Abstract:''' ''mariner'' family transposons are widespread<br />
among eukaryotic organisms. These transposons are apparently<br />
horizontally transmitted among diverse eukaryotes and<br />
can also transpose in vitro in the absence of added cofactors.<br />
Here we show that transposons derived from the ''mariner''<br />
element ''Himar1'' can efficiently transpose in bacteria ''in vivo''.<br />
We have developed simple transposition systems by using<br />
minitransposons, made up of short inverted repeats flanking<br />
antibiotic resistance markers. These elements can efficiently<br />
transpose after expression of transposase from an appropriate<br />
bacterial promoter. We found that transposition of ''mariner''-<br />
based elements in ''Escherichia coli'' produces diverse insertion<br />
mutations in either a targeted plasmid or a chromosomal<br />
gene. With ''Himar1''-derived transposons we were able to<br />
isolate phage-resistant mutants of both ''E. coli'' and ''Mycobacterium<br />
smegmatis''. ''mariner''-based transposons will provide<br />
valuable tools for mutagenesis and genetic manipulation of<br />
bacteria that currently lack well developed genetic systems.<br />
<br />
<br />
'''Link:''' [http://www.pnas.org/content/96/4/1645.abstract PNAS]</div>
Wiltshir
http://2009.igem.org/Team:Alberta/References/Publications/Gene_splicing_and_mutagenesis_by_PCR_driven_overlap_extension
Team:Alberta/References/Publications/Gene splicing and mutagenesis by PCR driven overlap extension
2009-10-20T07:12:26Z
<p>Wiltshir: New page: Karin L. Heckman and Larry R. Pease Nature Protocols 2, - 924 - 932 (2007) <b>Abstract:</b> Extension of overlapping gene segments by PCR is a simple, versatile technique for site-dire...</p>
<hr />
<div>Karin L. Heckman and Larry R. Pease<br />
<br />
Nature Protocols 2, - 924 - 932 (2007) <br />
<br />
<br />
<b>Abstract:</b> Extension of overlapping gene segments by PCR is a simple, versatile technique for site-directed mutagenesis and gene splicing. Initial PCRs generate overlapping gene segments that are then used as template DNA for another PCR to create a full-length product. Internal primers generate overlapping, complementary 3' ends on the intermediate segments and introduce nucleotide substitutions, insertions or deletions for site-directed mutagenesis, or for gene splicing, encode the nucleotides found at the junction of adjoining gene segments. Overlapping strands of these intermediate products hybridize at this 3' region in a subsequent PCR and are extended to generate the full-length product amplified by flanking primers that can include restriction enzyme sites for inserting the product into an expression vector for cloning purposes. The highly efficient generation of mutant or chimeric genes by this method can easily be accomplished with standard laboratory reagents in approximately 1 week.<br />
<br />
<br />
<br />
'''Link:''' [http://www.nature.com/nprot/journal/v2/n4/full/nprot.2007.132.html Nature]</div>
Wiltshir