http://2009.igem.org/wiki/index.php?title=Special:Contributions/Yashas&feed=atom&limit=50&target=Yashas&year=&month=2009.igem.org - User contributions [en]2024-03-28T15:28:56ZFrom 2009.igem.orgMediaWiki 1.16.5http://2009.igem.org/Team:ArtScienceBangalore/TeamTeam:ArtScienceBangalore/Team2009-10-22T01:45:37Z<p>Yashas: </p>
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<p>ArtScienceBangalore consists of ten undergraduate art and design students in their 2nd or 3rd years of study at the <a href="http://www.srishti.ac.in">Srishti School of Art, Design and Technology</a>. We have been assisted, guided and mentored by Yashas Shetty, an artist-in-residence at the Centre for Experimental Media Arts <a href="http://cema.srishti.ac.in/content/bioart">(CEMA) </a>and the National Centre for Biological Sciences <a href="http://www.ncbs.res.in/">(NCBS)</a> along with Navneet Rai,a graduate student and Dr. Mukund Thattai,Assistant Professor also from NCBS.<br />
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[[Image:Caricatswithnames.png|right]]<br />
[http://hackteria.org/wiki/index.php/User:Navadrian.cinthus Akash Hirosh]<br />
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[http://hackteria.org/wiki/index.php/Avni_Sethi Avni Sethi]<br />
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[http://hackteria.org/wiki/index.php/User:Iamnosuperman Dhruv Nawani]<br />
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[http://hackteria.org/wiki/index.php/User:Gautamf472 Gautam Vishwanath]<br />
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[http://hackteria.org/wiki/index.php/Krupakar_Dhinakaran Krupakar Dhinakaran]<br />
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[http://hackteria.org/wiki/index.php/User:NehaBhat Neha Bhat]<br />
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[http://hackteria.org/wiki/index.php/Nikhil_Patil Nikhil Patil]<br />
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[http://hackteria.org/wiki/index.php/User:Sem2490 Sandeep Mathew]<br />
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[http://hackteria.org/wiki/index.php/Sanya_Rai_Gupta Sanya Rai Gupta]<br />
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[http://hackteria.org/wiki/index.php/Upasana_Simha Upasana Simha]<br />
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Yashas Shetty<br />
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Mukund Thattai<br />
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Navneet Rai<br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/AproachTeam:ArtScienceBangalore/Aproach2009-10-21T17:08:31Z<p>Yashas: </p>
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<h1>Our Approach</h1><br />
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We consider ourselves amateurs/novices within the context of the IGEM competition. Our endeavor as “outsiders” is to bring our training in the arts and design to synthetic biology. Over this summer, we learnt the tools and techniques of synthetic biology and developed a piece of life which reflects our concerns, namely, the cultural, ethical and aesthetic implications of Synthetic Biology. Using a <a href ="http://hackteria.org/wiki/index.php/Main_Page" >DIY</a> approach and getting our hands “wet” was a critical element in the learning process. Our construct synthesizes Geosmin, an enzyme normally produced by cyanobacteria and actinobacteria. The biosynthesis of geosmin from farnesyl diphosphate is catalyzed by a single enzyme germacradienol/germacrene D synthase.E. coli, does not bear a gene that codes for this enzyme. We have expressed this gene in different strains of E. coli. Geosmin is responsible for producing the earthy smell when rain falls after a dry spell of weather.<br />
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<strong>Some of our Initial Brain Storms</strong><br />
[[Image:Whiteboard.jpg|820px|none|Click for a closer look]] <br />
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<strong>The Things we did over the Summer</strong><br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/AproachTeam:ArtScienceBangalore/Aproach2009-10-21T17:07:03Z<p>Yashas: </p>
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We consider ourselves amateurs/novices within the context of the IGEM competition. Our endeavor as “outsiders” is to bring our training in the arts and design to synthetic biology. Over this summer, we learnt the tools and techniques of synthetic biology and developed a piece of life which reflects our concerns, namely, the cultural, ethical and aesthetic implications of Synthetic Biology. Using a [http://hackteria.org/wiki/index.php/Main_Page DIY] approach and getting our hands “wet” was a critical element in the learning process. Our construct synthesizes Geosmin, an enzyme normally produced by cyanobacteria and actinobacteria. The biosynthesis of geosmin from farnesyl diphosphate is catalyzed by a single enzyme germacradienol/germacrene D synthase.E. coli, does not bear a gene that codes for this enzyme. We have expressed this gene in different strains of E. coli. Geosmin is responsible for producing the earthy smell when rain falls after a dry spell of weather.<br />
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<strong>Some of our Initial Brain Storms</strong><br />
[[Image:Whiteboard.jpg|820px|none|Click for a closer look]] <br />
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<strong>The Things we did over the Summer</strong><br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/NotebookTeam:ArtScienceBangalore/Notebook2009-10-21T17:03:40Z<p>Yashas: /* About */</p>
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== About ==<br />
We consider ourselves amateurs/novices within the context of the IGEM competition. Our endeavor as “outsiders” is to bring our training in the arts and design to synthetic biology. Over this summer, we learnt the tools and techniques of synthetic biology and developed a piece of life which reflects our concerns, namely, the cultural, ethical and aesthetic implications of Synthetic Biology. Using a [http://hackteria.org/wiki/index.php/Main_Page DIY] approach and getting our hands “wet” was a critical element in the learning process. This approach allowed us to engage in constructive discussions with scientists working in the field. Our construct synthesizes Geosmin, an enzyme normally produced by cyanobacteria and actinobacteria. The biosynthesis of geosmin from farnesyl diphosphate is catalyzed by a single enzyme germacradienol/germacrene D synthase. E. coli, does not bear a gene that codes for germacradienol/germacrene D synthase. We have expressed this gene in different strains of E. coli, under control of the pTrc promoter present in the pTrc99a vector. Geosmin is responsible for producing the earthy smell that occurs in the air when rain falls after a dry spell of weather.<br />
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== People ==<br />
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[[User:navadrian.cinthus|Akash Hirosh]]<br />
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[[User:iamnosuperman|Dhruv Nawani]]<br />
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[[Nikhil Patil]]<br />
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[[Upasana Simha]]<br />
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[[User:Sem2490|Sandeep Mathew]]<br />
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[[Sanya Rai Gupta]]<br />
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[[Avni Sethi]]<br />
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[[User:NehaBhat|Neha Bhat]]<br />
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[[User:Gautamf472|Gautam Vishwanath]]<br />
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[[Krupakar Dhinakaran]]<br />
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==Ideas==<br />
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[[File:Whiteboard.jpg|820px|none|Click for a closer look]] <br />
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Major Outcomes -<br />
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* [[Ideas for Bacteria.]]<br />
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* Facilitate a group of children to design their 'perfect creature' through visual/tactile media and then draw from these experiences and ideas to build another structure.<br />
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*Basic information on micro biology-synthetic biology becomes accessible in the forms of brochures/charts/posters in the team's work space.<br />
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== Workshop ==<br />
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{| border="0" cellspacing="0" cellpadding="4" align="center"<br />
![[Team:ArtScienceBangalore/Notebook/Week_One | Week One]]<br />
![[Team:ArtScienceBangalore/Notebook/Week_Two | Week Two]]<br />
![[Team:ArtScienceBangalore/Notebook/Week_Three | Week Three]]<br />
![[Team:ArtScienceBangalore/Notebook/Week_Four | Week Four]]<br />
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![[Week Five]]<br />
![[Week Six]]<br />
![[Week Seven]]<br />
![[Week Eight]]<br />
|}<br />
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=== Other ===<br />
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http://www.historyforkids.org/scienceforkids/biology/cells/doing/dna.htm<br />
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It is helpful to have an idea of how the other teams are progressing. So, if anyone comes across interesting iGEM team wikis/sites - please put up the links here.<br />
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http://igem.uwaterloo.ca/The_UW_iGEM_Team<br />
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The IBB Pune Team's wiki<br />
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[https://2009.igem.org/Team:IBB_Pune#]<br />
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[https://2008.igem.org/Team:NYMU-Taipei NYMU-Taipei's 2008 Wiki]<br />
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== Readings ==<br />
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'''[[Srishti_ArtandPolitics|Art and Politics]]'''<br />
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'''[[Srishti_Art|Art]]'''<br />
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'''[[Srishti_Science|Science]]'''<br />
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'''[[Srishti_Ethics|Ethics]]'''<br />
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'''[[Srishti_Design&Technology|Design & Technology]]'''<br />
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'''[[Srishti_SyntheticBiology|Synthetic Biology]]'''<br />
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'''[[Srishti_GeneralDesignLinks|General Design Links]]'''<br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/GalleryTeam:ArtScienceBangalore/Gallery2009-10-21T16:48:06Z<p>Yashas: </p>
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<p> <strong>Initial Exploration</strong> </p><br />
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[[Image:IMG 5818 -Desktop Resolution-.JPG|169px|BrainStorming]] [[Image:IMG 5747 -Desktop Resolution-.JPG|200px|Is a Chicken in a kaleidoscope,ethical Bioart?]] [[Image:IMG 5755 -Desktop Resolution-.JPG|200px|Avni's Sculpture evokes fierce debate on Ethics]] [[Image:IMG_5836_-Desktop_Resolution-.JPG|200px|Sandeep's sculpture-musturd on Cotton Seeds]]<br />
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<p> <strong>Experiments at Srishti</strong> </p><br />
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[[Image:INS3.jpg|150px|Our 25$ DIY Gel Electrophoresis Machine in Action ]] [[Image:IMG_5715_-Desktop_Resolution-.JPG|169px|Yes!we started IGEM with this Children's Microbe set]] [[Image:IMG_5726_-Desktop_Resolution-.JPG|200px|Growing Yeast]] [[Image:INS2.jpg|223px| Testing our Gel Electrophoresis Machine]]<br />
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<p> <strong>Experiments at NCBS</strong> </p><br />
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[[Image:IMG_5935_-Desktop_Resolution-.JPG|169px]] [[Image:IMG_5937_-Desktop_Resolution-.JPG|200px]] [[Image:DSC_8178.JPG|200px]] [[Image:DSC_8218.jpg|200px]] [[Image:DSC_8333.jpg|200px]]<br />
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<p> <strong>Workshop at Drishya</strong> </p><br />
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[[Image:Nik2small.jpg|150px|ASking Children to conceive a creature]] [[Image:DSC_8918small.jpg|223px|Talking about Genes and Genetics]] [[Image:DSC_8953small.jpg|223px|Debating Ethics]] <br />
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<p> <strong>Workshop at NID Bangalore</strong> </p><br />
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[[Image:DIY3.JPG|169px|DIY microscope wrapped in Paper]] [[Image:SDC12438.JPG|169px|Hacking a webcam]] [[Image:DIY4.JPG|200px|PureData Patch for our Microscope]]<br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/GalleryTeam:ArtScienceBangalore/Gallery2009-10-21T16:46:58Z<p>Yashas: </p>
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<p> <strong>Initial Exploration</strong> </p><br />
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[[Image:IMG 5818 -Desktop Resolution-.JPG|169px|BrainStorming]] [[Image:IMG 5747 -Desktop Resolution-.JPG|200px|Is a Chicken in a kaleidoscope,ethical Bioart?]] [[Image:IMG 5755 -Desktop Resolution-.JPG|200px|Avni's Sculpture evokes fierce debate on Ethics]] [[Image:IMG_5836_-Desktop_Resolution-.JPG|200px|Sandeep's sculpture-musturd on Cotton Seeds]]<br />
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<p> <strong>Experiments at Srishti</strong> </p><br />
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[[Image:INS3.jpg|150px|Our 25$ DIY Gel Electrophoresis Machine in Action ]] [[Image:IMG_5715_-Desktop_Resolution-.JPG|169px|Yes!we started IGEM with this Children's Microbe set]] [[Image:IMG_5726_-Desktop_Resolution-.JPG|200px|Growing Yeast]] [[Image:INS2.jpg|223px| Testing our Gel Electrophoresis Machine]]<br />
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<p> <strong>Experiments at NCBS</strong> </p><br />
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[[Image:IMG_5935_-Desktop_Resolution-.JPG|169px]] [[Image:IMG_5937_-Desktop_Resolution-.JPG|200px]] [[Image:DSC_8178.JPG|200px]] [[Image:DSC_8218.jpg|200px]] [[Image:DSC_8333.jpg|200px]]<br />
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<p> <strong>Workshop at Drishya</strong> </p><br />
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[[Image:Nik2small.jpg|150px|ASking Children to conceive a creature]] [[Image:DSC_8918small.jpg|223px|Talking about Genes and Genetics]] [[Image:DSC_8953small.jpg|223px|Debating Ethics]] <br />
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<p> <strong>Workshop at NID Bangalore</strong> </p><br />
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[[Image:DIY3.JPG|169px|Looking for Samples]] [[Image:SDC12438.JPG|169px|Hacking a webcam]] [[Image:DIY4.JPG|200px|PureData Patch for our Microscope]]<br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/GalleryTeam:ArtScienceBangalore/Gallery2009-10-21T16:44:31Z<p>Yashas: </p>
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<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Project">The Project</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Notebook">Notebook</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Outreach">Outreach</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Links">Links</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Parts">Parts</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Safety">Safety</a></li><br />
</ul><br />
</div><br />
<br />
<div id="asContent"><br />
<p> <strong>Initial Exploration</strong> </p><br />
</html><br />
<br />
[[Image:IMG 5818 -Desktop Resolution-.JPG|169px|BrainStorming]] [[Image:IMG 5747 -Desktop Resolution-.JPG|200px|Is a Chicken in a kaleidoscope,ethical Bioart?]] [[Image:IMG 5755 -Desktop Resolution-.JPG|200px|Avni's Sculpture evokes fierce debate on Ethics]] [[Image:IMG_5836_-Desktop_Resolution-.JPG|200px|Sandeep's sculpture-musturd on Cotton Seeds]]<br />
<html><br />
<p> <strong>Experiments at Srishti</strong> </p><br />
</html><br />
[[Image:INS3.jpg|150px|Our 25$ DIY Gel Electrophoresis Machine in Action ]] [[Image:IMG_5715_-Desktop_Resolution-.JPG|169px|Yes!we started IGEM with this Children's Microbe set]] [[Image:IMG_5726_-Desktop_Resolution-.JPG|200px|Growing Yeast]] [[Image:INS2.jpg|223px| Testing our Gel Electrophoresis Machine] <br />
<html><br />
<p> <strong>Experiments at NCBS</strong> </p><br />
<p> <strong>Workshop at Drishya</strong> </p><br />
</html><br />
[[Image:Nik2small.jpg|150px|Caption]] [[Image:DSC_8918small.jpg|223px|Caption]] [[Image:DSC_8953small.jpg|223px|Caption]] <br />
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<p> <strong>Workshop at NID Bangalore</strong> </p><br />
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[[Image:DIY3.JPG|169px|Caption]] [[Image:SDC12438.JPG|169px|Caption]] [[Image:DIY4.JPG|200px|Caption]]<br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/GalleryTeam:ArtScienceBangalore/Gallery2009-10-21T16:42:37Z<p>Yashas: </p>
<hr />
<div><html><br />
<link rel="stylesheet" href="http://hackteria.org/as.css" type="text/css" /><br />
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<h1>Gallery</h1><br />
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<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Parts">Parts</a></li><br />
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</ul><br />
</div><br />
<br />
<div id="asContent"><br />
<p> <strong>Initial Exploration</strong> </p><br />
</html><br />
<br />
[[Image:IMG 5818 -Desktop Resolution-.JPG|169px|BrainStorming]] [[Image:IMG 5747 -Desktop Resolution-.JPG|200px|Is a Chicken in a kaleidoscope,ethical Bioart?]] [[Image:IMG 5755 -Desktop Resolution-.JPG|200px|Avni's Sculpture evokes fierce debate on Ethics]] [[Image:IMG_5836_-Desktop_Resolution-.JPG|200px|Sandeep's sculpture-musturd on Cotton Seeds]]<br />
<html><br />
<p> <strong>Experiments at Srishti</strong> </p><br />
</html><br />
[[Image:INS3.jpg|150px|Caption]] [[Image:IMG_5715_-Desktop_Resolution-.JPG|169px|Caption]] [[Image:IMG_5726_-Desktop_Resolution-.JPG|200px|Caption]] [[Image:INS2.jpg|223px|Caption]] <br />
<html><br />
<p> <strong>Experiments at NCBS</strong> </p><br />
<p> <strong>Workshop at Drishya</strong> </p><br />
</html><br />
[[Image:Nik2small.jpg|150px|Caption]] [[Image:DSC_8918small.jpg|223px|Caption]] [[Image:DSC_8953small.jpg|223px|Caption]] <br />
<html><br />
<p> <strong>Workshop at NID Bangalore</strong> </p><br />
</html><br />
[[Image:DIY3.JPG|169px|Caption]] [[Image:SDC12438.JPG|169px|Caption]] [[Image:DIY4.JPG|200px|Caption]]<br />
<html><br />
<br />
</div><br />
<br />
</div><br />
</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/OutreachTeam:ArtScienceBangalore/Outreach2009-10-21T15:29:52Z<p>Yashas: </p>
<hr />
<div><html><br />
<link rel="stylesheet" href="http://hackteria.org/as.css" type="text/css" /><br />
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<br />
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<ul><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore">Home</a></li><br />
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<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Parts">Parts</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Safety">Safety</a></li><br />
</ul><br />
</div><br />
<br />
<div id="asContent"><br />
<p><br />
<br />
</p><br />
<br />
<p><br />
Through the summer, we went around schools and colleges and tried to explain what we were doing. From schools for the urban poor(Drishya) to other design schools(NID). Most of them were intrigued and wanted to participate. We have a <a href="http://www.facebook.com/group.php?gid=94836276980">facebook page</a> and hopefully we'll have more discussions happen there. We also helped create an Online resource for artists/designers that want to get involved with Biotechnology. It's called <a href="http://hackteria.org">Hackteria</a>.We are also designing a creative commons/open source Synthetic Biology handbook for Artists and Designers. You can access that <a href="http://hackteria.org/wiki/index.php/File:Handbook.pdf">here</a>.<br />
</p><br />
<br />
<p><br />
<strong>Talking to Drishya about genetic engineering and synthetic biology</strong><br />
</p><br />
</html><br />
[[Image:Crowd.jpg|400px]] [[Image:DSC_8918small.jpg|400px]] <br />
<html><br />
<p><br />
<strong>Doing the DIY Microscopy workshop at NID, Bangalore</strong><br />
</p><br />
</html><br />
[[Image:DIY1.JPG|400px]][[Image:DIY2.JPG|400px]]<br />
<html><br />
<br />
</div><br />
<br />
</div><br />
</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/AproachTeam:ArtScienceBangalore/Aproach2009-10-21T14:03:56Z<p>Yashas: </p>
<hr />
<div><html><br />
<link rel="stylesheet" href="http://hackteria.org/as.css" type="text/css" /><br />
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<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Notebook">Notebook</a></li><br />
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<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Parts">Parts</a></li><br />
</ul><br />
</div><br />
<br />
<div id="asContent"><br />
<br />
<br />
<p><br />
We consider ourselves amateurs/novices within the context of the IGEM competition. Our endeavor as “outsiders” is to bring our training in the arts and design to synthetic biology. Over this summer, we learnt the tools and techniques of synthetic biology and developed a piece of life which reflects our concerns, namely, the cultural, ethical and aesthetic implications of Synthetic Biology. Using a DIY approach and getting our hands “wet” was a critical element in the learning process. Our construct synthesizes Geosmin, an enzyme normally produced by cyanobacteria and actinobacteria. The biosynthesis of geosmin from farnesyl diphosphate is catalyzed by a single enzyme germacradienol/germacrene D synthase.E. coli, does not bear a gene that codes for this enzyme. We have expressed this gene in different strains of E. coli. Geosmin is responsible for producing the earthy smell when rain falls after a dry spell of weather.<br />
<br />
</p><br />
<br />
</html><br />
<br />
<strong>Some of our Initial Brain Storms</strong><br />
[[Image:Whiteboard.jpg|820px|none|Click for a closer look]] <br />
<html><br />
<br />
<br />
<strong>The Things we did over the Summer</strong><br />
<object width="640" height="505"><param name="movie" value="http://www.youtube.com/v/jmwUdYpy72g&hl=en&fs=1&"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="http://www.youtube.com/v/jmwUdYpy72g&hl=en&fs=1&" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="640" height="505"></embed></object><br />
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</p><br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/AproachTeam:ArtScienceBangalore/Aproach2009-10-21T11:36:18Z<p>Yashas: </p>
<hr />
<div><html><br />
<link rel="stylesheet" href="http://hackteria.org/as.css" type="text/css" /><br />
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<h1>Approach</h1><br />
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<div id="asMenu"><br />
<ul><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore">Home</a></li><br />
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<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Notebook">Notebook</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Outreach">Outreach</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Links">Links</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Gallery">Gallery</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Safety">Safety</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Parts">Parts</a></li><br />
</ul><br />
</div><br />
<br />
<div id="asContent"><br />
<br />
<strong>Our Approach</strong><br />
<p><br />
We consider ourselves amateurs/novices within the context of the IGEM competition. Our endeavor as “outsiders” is to bring our training in the arts and design to synthetic biology. Over this summer, we learnt the tools and techniques of synthetic biology and developed a piece of life which reflects our concerns, namely, the cultural, ethical and aesthetic implications of Synthetic Biology. Using a DIY approach and getting our hands “wet” was a critical element in the learning process. Our construct synthesizes Geosmin, an enzyme normally produced by cyanobacteria and actinobacteria. The biosynthesis of geosmin from farnesyl diphosphate is catalyzed by a single enzyme germacradienol/germacrene D synthase.E. coli, does not bear a gene that codes for this enzyme. We have expressed this gene in different strains of E. coli. Geosmin is responsible for producing the earthy smell when rain falls after a dry spell of weather.<br />
<br />
</p><br />
<br />
<br />
<br />
<br />
<object width="640" height="505"><param name="movie" value="http://www.youtube.com/v/jmwUdYpy72g&hl=en&fs=1&"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="http://www.youtube.com/v/jmwUdYpy72g&hl=en&fs=1&" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="640" height="505"></embed></object><br />
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[[Image:Whiteboard.jpg|820px|none|Click for a closer look]] <br />
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</p><br />
<p><br />
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</p><br />
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</p><br />
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</p><br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/OutreachTeam:ArtScienceBangalore/Outreach2009-10-21T11:26:14Z<p>Yashas: </p>
<hr />
<div><html><br />
<link rel="stylesheet" href="http://hackteria.org/as.css" type="text/css" /><br />
<div id="asMain"><br />
<div id="asTitle"><br />
<h1>the project: untitled</h1><br />
</div><br />
<br />
<div id="asMenu"><br />
<ul><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore">Home</a></li><br />
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<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Aproach">Approach</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Project">The Project</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Notebook">Notebook</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Links">Links</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Gallery">Gallery</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Parts">Parts</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Safety">Safety</a></li><br />
</ul><br />
</div><br />
<br />
<div id="asContent"><br />
<p><br />
<strong> Outreach</Strong><br />
</p><br />
<br />
<p><br />
Through the summer, we went around schools and colleges and tried to explain what we were doing. From schools for the urban poor(Drishya) to other design schools(NID). Most of them were intrigued wanted to participate. We have a <a href="http://www.facebook.com/group.php?gid=94836276980">facebook page</a> and hopefully we'll have more discussions happen there. We are also designing a creative commons/open source Synthetic Biology handbook for Artists and Designers. You can access that <a href="http://hackteria.org/wiki/index.php/File:Handbook.pdf">here</a>.<br />
</p><br />
<br />
<p><br />
<strong>Talking to Drishya about genetic engineering and synthetic biology</strong><br />
</p><br />
<p><br />
<strong>Doing the DIY Microscopy workshop at NID, Bangalore</strong><br />
</p><br />
<br />
</div><br />
<br />
</div><br />
</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/OutreachTeam:ArtScienceBangalore/Outreach2009-10-21T11:24:32Z<p>Yashas: </p>
<hr />
<div><html><br />
<link rel="stylesheet" href="http://hackteria.org/as.css" type="text/css" /><br />
<div id="asMain"><br />
<div id="asTitle"><br />
<h1>the project: untitled</h1><br />
</div><br />
<br />
<div id="asMenu"><br />
<ul><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore">Home</a></li><br />
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<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Project">The Project</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Notebook">Notebook</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Links">Links</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Gallery">Gallery</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Parts">Parts</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Safety">Safety</a></li><br />
</ul><br />
</div><br />
<br />
<div id="asContent"><br />
<p><br />
<strong> Outreach</Strong><br />
</p><br />
<br />
<p><br />
Through the summer, we went around schools and colleges and tried to explain what we were doing. From schools for the urban poor(Drishya) to other design schools(NID). Most of them were intrigued wanted to participate. We have a <a href="http://www.facebook.com/group.php?gid=94836276980">facebook page</a> and hopefully we'll have more discussions happen there. We are also designing a creative commons/open source Synthetic Biology handbook for Artists and Designers. You can access that <a href="http://hackteria.org/wiki/index.php/File:Handbook.pdf">here</a>.<br />
</p><br />
<br />
<br />
<br />
<br />
</div><br />
<br />
</div><br />
</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/OutreachTeam:ArtScienceBangalore/Outreach2009-10-21T11:21:56Z<p>Yashas: </p>
<hr />
<div><html><br />
<link rel="stylesheet" href="http://hackteria.org/as.css" type="text/css" /><br />
<div id="asMain"><br />
<div id="asTitle"><br />
<h1>the project: untitled</h1><br />
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<br />
<div id="asMenu"><br />
<ul><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore">Home</a></li><br />
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<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Notebook">Notebook</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Links">Links</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Gallery">Gallery</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Parts">Parts</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Safety">Safety</a></li><br />
</ul><br />
</div><br />
<br />
<div id="asContent"><br />
<p><br />
<strong> Outreach</Strong><br />
</p><br />
<br />
<p><br />
Through the summer, we went around schools and colleges and tried to explain what we were doing. From schools for the urban poor(Drishya) to other design schools(NID). Most of them were intrigued wanted to participate. We have a facebook page and hopefully we'll have more discussions happen there. We are also designing a creative commons/open source Synthetic Biology handbook for Artists and Designers. You can access that <a href="http://hackteria.org/wiki/index.php/File:Handbook.pdf">here</a>.<br />
</p><br />
<br />
<br />
<br />
<br />
</div><br />
<br />
</div><br />
</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/PartsTeam:ArtScienceBangalore/Parts2009-10-21T11:20:27Z<p>Yashas: </p>
<hr />
<div><html><br />
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<ul><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore">Home</a></li><br />
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<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Project">The Project</a></li><br />
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</ul><br />
</div><br />
<br />
<div id="asContent"><br />
<br />
<strong>Parts</strong><br />
<br />
Here is the part that we submitted to the registry. <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K221000">BBa_K221000</a><br />
This helps our construct synthasize geosmin, the enzyme that smells like rain.<br />
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</div><br />
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</div><br />
</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/OutreachTeam:ArtScienceBangalore/Outreach2009-10-21T11:14:54Z<p>Yashas: </p>
<hr />
<div><html><br />
<link rel="stylesheet" href="http://hackteria.org/as.css" type="text/css" /><br />
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<li><a href="https://2009.igem.org/Team:ArtScienceBangalore">Home</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Team">The Team</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Aproach">Approach</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Project">The Project</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Notebook">Notebook</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Outreach">Outreach</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Links">Links</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Gallery">Gallery</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Parts">Parts</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Safety">Safety</a></li><br />
</ul><br />
</div><br />
<br />
<div id="asContent"><br />
<strong> Outreach</Strong><br />
<br />
Through the summer, we went around schools and colleges and tried to explain what we were doing. From schools for the urban poor(Drishya) to other design schools(NID). Most of them were intrigued wanted to participate. We have a facebook page and hopefully we'll have more discussions happen there. We are also designing a creative commons/open source Synthetic Biology handbook for Artists and Designers. You can access that here.[http://hackteria.org/wiki/index.php/File:Handbook.pdf]<br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/OutreachTeam:ArtScienceBangalore/Outreach2009-10-21T11:13:42Z<p>Yashas: </p>
<hr />
<div><html><br />
<link rel="stylesheet" href="http://hackteria.org/as.css" type="text/css" /><br />
<div id="asMain"><br />
<div id="asTitle"><br />
<h1>the project: untitled</h1><br />
</div><br />
<br />
<div id="asMenu"><br />
<ul><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore">Home</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Team">The Team</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Aproach">Approach</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Project">The Project</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Notebook">Notebook</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Outreach">Outreach</a></li><br />
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<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Safety">Safety</a></li><br />
</ul><br />
</div><br />
<br />
<div id="asContent"><br />
<strong> Outreach</Strong><br />
<br />
Through the summer, we went around schools and colleges and tried to explain what we were doing. From schools for the urban poor(Drishya) to other design schools(NID). Most of them were intrigued wanted to participate. We have a facebook page and hopefully we'll have more discussions happen there. We are also designing a creative commons/open source Synthetic Biology handbook for Artists and Designers. You can access that here.[http://hackteria.org/wiki/index.php/File:Handbook.pdf]<br />
<br />
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</div><br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/SafetyTeam:ArtScienceBangalore/Safety2009-10-21T10:52:13Z<p>Yashas: /* Safety */</p>
<hr />
<div>== Safety ==<br />
<br />
1. None of the ideas raised saftey issues inside or outside the laboratory.<br />
<br />
2. The Bench work was done inside the National Center for Biological Sciences(bangalore) premises and strictly followed the saftey protocols and guidelines set by the saftey review board at NCBS.<br />
<br />
3. At National Center for Biological Sciences, Bangalore, we have well equipped lab and we take care of biosafety properly. Current facilities at our lab full-fill the requirements of biosafety level-1 as mentioned in Biosafety in Microbiological and Biomedical Laboratories (4th edition, U.S. Department of Health and Human Services Public Health Service, Centers for Disease Control and Prevention and National Institutes of Health, U.S. Government Printing Office Washington: 1999).We have an Institute level committee, who looks in to the issues related to biosafety. Our part BBa_K221000 produces a harmless and eco-friendly organic compound “Geosmin” and since we follow all standard lab practices, biosafety committee does not has any issue with our experiments. We follow following standard lab practices, regularly:<br />
<br />
4. None of the BioBrick parts raise any safety issues.</div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/SafetyTeam:ArtScienceBangalore/Safety2009-10-21T10:50:56Z<p>Yashas: /* Saftey */</p>
<hr />
<div>== Safety ==<br />
<br />
1. None of the ideas raised saftey issues inside or outside the laboratory.<br />
2. The Bench work was done inside the National Center for Biological Sciences(bangalore) premises and strictly followed the saftey protocols and guidelines set by the saftey review board at NCBS.<br />
3. The saftey review board did not have a problem with the project.<br />
4. None of the BioBrick parts raise any safety issues.</div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/SafetyTeam:ArtScienceBangalore/Safety2009-10-21T10:50:27Z<p>Yashas: /* Saftey */</p>
<hr />
<div><br />
== Safeyy ==<br />
<br />
1. None of the ideas raised saftey issues inside or outside the laboratory.<br />
2. The Bench work was done inside the National Center for Biological Sciences(bangalore) premises and strictly followed the saftey protocols and guidelines set by the saftey review board at NCBS.<br />
3. The saftey review board did not have a problem with the project.<br />
4. None of the BioBrick parts raise any safety issues.</div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/SafetyTeam:ArtScienceBangalore/Safety2009-10-21T10:48:05Z<p>Yashas: New page: == Saftey == 1. None of the ideas raised saftey issues inside or outside the laboratory. 2. The Bench work was done inside the National Center for Biological Sciences(bangalore) premises...</p>
<hr />
<div><br />
== Saftey ==<br />
<br />
1. None of the ideas raised saftey issues inside or outside the laboratory.<br />
2. The Bench work was done inside the National Center for Biological Sciences(bangalore) premises and strictly followed the saftey protocols and guidelines set by the saftey review board at NCBS.<br />
3. The saftey review board did not have a problem with the project.<br />
4. None of the BioBrick parts raise any safety issues.</div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/AproachTeam:ArtScienceBangalore/Aproach2009-10-21T10:43:03Z<p>Yashas: </p>
<hr />
<div><html><br />
<link rel="stylesheet" href="http://hackteria.org/as.css" type="text/css" /><br />
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<h1>Approach</h1><br />
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<div id="asMenu"><br />
<ul><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore">Home</a></li><br />
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<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Project">The Project</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Notebook">Notebook</a></li><br />
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</ul><br />
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<div id="asContent"><br />
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[[Image:Whiteboard.jpg|820px|none|Click for a closer look]] <br />
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<p><br />
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<br />
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<p><br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/TeamTeam:ArtScienceBangalore/Team2009-10-21T10:32:03Z<p>Yashas: </p>
<hr />
<div><html><br />
<link rel="stylesheet" href="http://hackteria.org/as.css" type="text/css" /><br />
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<h1>Team</h1><br />
</div><br />
<br />
<div id="asMenu"><br />
<ul><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore">Home</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Team">The Team</a></li><br />
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<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Project">The Project</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Notebook">Notebook</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Outreach">Outreach</a></li><br />
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<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Parts">Parts</a></li><br />
</ul><br />
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<div id="asContent"><br />
<br />
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<p><br />
We are second and third year students of Art and Design from the <a href="http://www.srishti.ac.in">Srishti School of Art, Design and Technology</a><br />
</p><br />
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[[Image:Photo_61.jpg|right|frame|From left to right:Gautam, Nikhil, Sandeep, Dhruv, Avni, Sanya, Krupakar(the guy behind), Upasana and Akash Invisible: Neha]] <br />
<br />
<br />
<br />
<br />
[http://hackteria.org/wiki/index.php/User:Navadrian.cinthus Akash Hirosh]<br />
<br />
[http://hackteria.org/wiki/index.php/Avni_Sethi Avni Sethi]<br />
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[http://hackteria.org/wiki/index.php/User:Iamnosuperman Dhruv Nawani]<br />
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[http://hackteria.org/wiki/index.php/User:Gautamf472 Gautam Vishwanath]<br />
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[http://hackteria.org/wiki/index.php/Krupakar_Dhinakaran Krupakar Dhinakaran]<br />
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[http://hackteria.org/wiki/index.php/User:NehaBhat Neha Bhat]<br />
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[http://hackteria.org/wiki/index.php/Nikhil_Patil Nikhil Patil]<br />
<br />
[http://hackteria.org/wiki/index.php/User:Sem2490 Sandeep Mathew]<br />
<br />
[http://hackteria.org/wiki/index.php/Sanya_Rai_Gupta Sanya Rai Gupta]<br />
<br />
[http://hackteria.org/wiki/index.php/Upasana_Simha Upasana Simha]<br />
<html><br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/TeamTeam:ArtScienceBangalore/Team2009-10-21T10:31:01Z<p>Yashas: </p>
<hr />
<div><html><br />
<link rel="stylesheet" href="http://hackteria.org/as.css" type="text/css" /><br />
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<ul><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore">Home</a></li><br />
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<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Project">The Project</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Notebook">Notebook</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Outreach">Outreach</a></li><br />
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<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Gallery">Gallery</a></li><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Parts">Parts</a></li><br />
</ul><br />
</div><br />
<br />
<div id="asContent"><br />
<br />
<br />
<p><br />
We are second and third year students of Art and Design from the <a href="www.srishti.ac.in">Srishti School of Art, Design and Technology</a><br />
</p><br />
<br />
</html><br />
<br />
[[Image:Photo_61.jpg|right|frame|From left to right:Gautam, Nikhil, Sandeep, Dhruv, Avni, Sanya, Krupakar(the guy behind), Upasana and Akash Invisible: Neha]] <br />
<br />
<br />
<br />
<br />
[http://hackteria.org/wiki/index.php/User:Navadrian.cinthus Akash Hirosh]<br />
<br />
[http://hackteria.org/wiki/index.php/Avni_Sethi Avni Sethi]<br />
<br />
[http://hackteria.org/wiki/index.php/User:Iamnosuperman Dhruv Nawani]<br />
<br />
[http://hackteria.org/wiki/index.php/User:Gautamf472 Gautam Vishwanath]<br />
<br />
[http://hackteria.org/wiki/index.php/Krupakar_Dhinakaran Krupakar Dhinakaran]<br />
<br />
[http://hackteria.org/wiki/index.php/User:NehaBhat Neha Bhat]<br />
<br />
[http://hackteria.org/wiki/index.php/Nikhil_Patil Nikhil Patil]<br />
<br />
[http://hackteria.org/wiki/index.php/User:Sem2490 Sandeep Mathew]<br />
<br />
[http://hackteria.org/wiki/index.php/Sanya_Rai_Gupta Sanya Rai Gupta]<br />
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[http://hackteria.org/wiki/index.php/Upasana_Simha Upasana Simha]<br />
<html><br />
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<br />
</div><br />
</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/PartsTeam:ArtScienceBangalore/Parts2009-10-21T10:26:42Z<p>Yashas: </p>
<hr />
<div><html><br />
<head><br />
<link href="https://2009.igem.org/wiki/index.php?title=User:Sem2490/wiki/sandeep.css&action=raw&ctype=text/css" rel="stylesheet" type="text/css"><br />
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<td><a href="https://2009.igem.org/Team:ArtScienceBangalore"> Home </a></td><br />
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<td><a href="https://2009.igem.org/Team:ArtScienceBangalore/Brainstorms"> Brainstorms</a></td><br />
<td><a href="https://2009.igem.org/Team:ArtScienceBangalore/Project"> The Project</a></td><br />
<td><a href="https://2009.igem.org/Team:ArtScienceBangalore/Notebook"> Notebook</a></td><br />
<td><a href="https://2009.igem.org/Team:ArtScienceBangalore/Outreach"> Outreach</a></td><br />
<td><a href="https://2009.igem.org/Team:ArtScienceBangalore/Links"> Links</a></td><br />
<td><a href="https://2009.igem.org/Team:ArtScienceBangalore/Gallery"> Gallery</a></td><br />
<br />
</tr><br />
</table><br />
</body><br />
</html><br />
<br />
<strong>Parts</strong><br />
<br />
Here is the part that we submitted to the registry. <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K221000">BBa_K221000</a><br />
This helps our construct synthasize geosmin, the enzyme that smells like rain.<br />
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<br />
===Note===<br />
<br />
If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here. This is not necessary but it may be a nice list to keep track of.</div>Yashashttp://2009.igem.org/Team:ArtScienceBangaloreTeam:ArtScienceBangalore2009-10-21T10:16:51Z<p>Yashas: </p>
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<img src="http://hackteria.org/postwiki.png" alt="ArtSciencBangalore" usemap="#asMap" ismap/><br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangaloreTeam:ArtScienceBangalore2009-10-21T10:09:32Z<p>Yashas: </p>
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<img src="http://hackteria.org/postwiki.png" alt="ArtSciencBangalore" usemap="#asMap" ismap/><br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/TeamTeam:ArtScienceBangalore/Team2009-10-21T10:05:33Z<p>Yashas: </p>
<hr />
<div><html><br />
<link rel="stylesheet" href="http://hackteria.org/as.css" type="text/css" /><br />
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<ul><br />
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore">Home</a></li><br />
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</html><br />
<br />
<li>We are second and third year students of Art and Design from the <a href="www.srishti.ac.in">Srishti School of Art, Design and Technology</a></li><br />
<br />
<br />
[[Image:Photo_61.jpg|right|frame|From left to right:Gautam, Nikhil, Sandeep, Dhruv, Avni, Sanya, Krupakar(the guy behind), Upasana and Akash Invisible: Neha]] <br />
<br />
<br />
<br />
<br />
[http://hackteria.org/wiki/index.php/User:Navadrian.cinthus Akash Hirosh]<br />
<br />
[http://hackteria.org/wiki/index.php/Avni_Sethi Avni Sethi]<br />
<br />
[http://hackteria.org/wiki/index.php/User:Iamnosuperman Dhruv Nawani]<br />
<br />
[http://hackteria.org/wiki/index.php/User:Gautamf472 Gautam Vishwanath]<br />
<br />
[http://hackteria.org/wiki/index.php/Krupakar_Dhinakaran Krupakar Dhinakaran]<br />
<br />
[http://hackteria.org/wiki/index.php/User:NehaBhat Neha Bhat]<br />
<br />
[http://hackteria.org/wiki/index.php/Nikhil_Patil Nikhil Patil]<br />
<br />
[http://hackteria.org/wiki/index.php/User:Sem2490 Sandeep Mathew]<br />
<br />
[http://hackteria.org/wiki/index.php/Sanya_Rai_Gupta Sanya Rai Gupta]<br />
<br />
[http://hackteria.org/wiki/index.php/Upasana_Simha Upasana Simha]<br />
<html><br />
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</div><br />
</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/TeamTeam:ArtScienceBangalore/Team2009-10-21T10:00:48Z<p>Yashas: </p>
<hr />
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We are second and third year students of Art and Design from the <li><a href="www.srishti.ac.in">Srishti School of Art, Design and Technology</a></li><br />
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[[Image:Photo_61.jpg|right|frame|From left to right:Gautam, Nikhil, Sandeep, Dhruv, Avni, Sanya, Krupakar(the guy behind), Upasana and Akash Invisible: Neha]] <br />
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[http://hackteria.org/wiki/index.php/User:Navadrian.cinthus Akash Hirosh]<br />
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[http://hackteria.org/wiki/index.php/Avni_Sethi Avni Sethi]<br />
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[http://hackteria.org/wiki/index.php/User:Iamnosuperman Dhruv Nawani]<br />
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[http://hackteria.org/wiki/index.php/User:Gautamf472 Gautam Vishwanath]<br />
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[http://hackteria.org/wiki/index.php/Krupakar_Dhinakaran Krupakar Dhinakaran]<br />
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[http://hackteria.org/wiki/index.php/User:NehaBhat Neha Bhat]<br />
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[http://hackteria.org/wiki/index.php/Nikhil_Patil Nikhil Patil]<br />
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[http://hackteria.org/wiki/index.php/User:Sem2490 Sandeep Mathew]<br />
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[http://hackteria.org/wiki/index.php/Sanya_Rai_Gupta Sanya Rai Gupta]<br />
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[http://hackteria.org/wiki/index.php/Upasana_Simha Upasana Simha]<br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/OutreachTeam:ArtScienceBangalore/Outreach2009-10-21T09:35:54Z<p>Yashas: </p>
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<strong> Outreach</Strong><br />
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Through the summer, we went around schools and colleges and tried to explain what we were doing. From schools for the urban poor(Drishya) to other design schools(NID). Most of them were intrigued wanted to participate. We have a facebook page and hopefully we'll have more discussions happen there.<br />
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<div>Experiments</div>Yashashttp://2009.igem.org/Team:ArtScienceBangaloreTeam:ArtScienceBangalore2009-10-21T06:26:40Z<p>Yashas: </p>
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<p><a href="http://srishti.ac.in/"> Srishti </a></p><br />
<p><a href="http://www.ncbs.res.in/index.php?option=com_content&task=view&id=349&Itemid=9"> NCBS </a></p><br />
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<p>Geosmin, which means ‘earth odor’, is a volatile microbial metabolite that is responsible for the characteristic smell of moist soil or freshly plowed earth. Geosmin is produced by a number of microorganisms, including most Streptomyces and several species of cyanobacteria, myxobacteria and fungi. Besides its pleasant, characteristic earthy aroma, geosmin is also associated with an undesirable musty odor or off flavor in drinking water, as well as in wine, fish, and other food stuffs. The structure of geosmin was first established as trans- 1,10-dimethyl-trans-9 decalol by N.N Gerber who detected the volatile oil in 17 different species of Streptomyces and a blue green alga following its initial isolation from S. griseus. </p><br />
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<p>Biosynthesis of geosmin from farnesyl diphosphate is catalyzed by a single enzyme germacradienol/germacrene D synthase. Escherichia coli: ispA codes for farnesyl diphosphate (FPP) synthase. FPP synthase catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (C5) and geranyl diphosphate (C10) to form FPP. </p><br />
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<p>E. coli, does not bear a gene that codes for germacradienol/germacrene D synthase. In our project, we are expressing germacradienol/germacrene D synthase gene in different strains of E. coli, under control of pTrc promoter present in pTrc99a vector (BBa_”---“). This is basically an expression vector (that contains a LacI repressor) which can be induced by IPTG. A map of this vector is given below :</p><br />
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<p>This synthase plasmid SF5 ( ispA+, triangle (srl-recA) 306::Tn10) encoding the whole protein is transformed to the full-length wild strain SF5 to produce Geosmin and eventually the ‘smell’ of rain. This is tested against a knockout strain SF7N ( triangle ispA::neo, triangle (srl-recA) 306 :: Tn10) that did not contain farnesyl synthase which is the precursor to the production of farnesyl diphosphate. Hence, when the plasmid is inoculated, no Geosmin will be expected to form.</p><br />
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<h3>Experimental protocol:</h3><br />
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<li>E. coli k12z1 bearing BBa_”---“and k12z1 was grown overnight at 37oC and 250rpm in Luria Bertani (LB) medium containing 100uM ampicillin and spectinomycin respectively.</li><br />
<li>BBa_”---“culture was inoculated in fresh 1X glucose-M9 medium, containing 0, 50, 500 and 1000uM of IPTG. AS a negative control K12z1 was inoculated in fresh 1X glucose-M9 medium.</li><br />
<li>The culture was grown for 12h at 37oC and 250rpm.</li><br />
<li>Geosmin analysis was done through smell, and it appeared that k12z1 cells bearing BBa_”--“, are geosmin positive.</li><br />
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<p>We have managed to obtain a mutated strain pSF27 (ispA+, cm R,pSC 101 Ori ts) from Dr. XX at Toho University, which is temperature- sensitive and using this, a controlled ispA strain is being constructed by transforming SF7N with pSF27 wherein on reducing the temperatures, synthesis of Geosmin takes place and the smell of rain is produced.<br />
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<h3>What did and did not work</h3><br />
<p>While working with the full length synthase plasmid SF5, and testing it with the knockout strain SF7N, no difference could be observed in the two resultant smells. From this we inferred that geosmin was not being synthesized as per our theoretical analysis.</p><br />
<ul><br />
<li>One reason for this could be the fact that the concentrations of farnesyl diphosphate may not be accurate and require further trial and error through experimentation.</li><br />
<li>It was also discovered that the C- terminal of the synthase contained a restriction enzyme which could well be the reason for the failure of our experiment. We also discovered that the expression of the N-terminal domain of the plasmid pTRC99a gave a fully functional germacradienol synthase with steady-state catalytic parameters similar to those of the full-length protein but the expressed C-terminal domain had no detectable FPP cyclase activity. Hence, it was only the N- terminal that was needed for the synthesis of geosmin and the C- terminal was the half that contained the restriction enzyme and as it played no part in the synthesis process, it could be deleted from the gene.</li><br />
</ul><br />
<br />
<img src="https://static.igem.org/mediawiki/2009/0/09/Geosmin_reaction.jpg" width="500px"></img><br />
<br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/AproachTeam:ArtScienceBangalore/Aproach2009-10-21T06:10:04Z<p>Yashas: </p>
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<p><br />
Focused on the ideology of making science accessible to the non-scientific community, we experimented with the Do-It-Yourself approach of working.<br />
</p><br />
<p><br />
We began with familiarizing ourselves with the nature of DIYBio and dipped our hands in beginner level scientific procedures that introduced us to the building blocks of the seemingly hurdle -filled stream that we were trying to pursue. From building our own gel electrophoresis chamber in our self constructed backyard bio lab in an Art and Design school campus to conducting gel electrophoresis in a professional lab, we played with the boundaries of the DIY style of working. A three time failure at an attempt to experiment with cell lysis followed and so did a deep insight into the skills of being a lab practitioner. We delved into process of science.<br />
</p><br />
<p><br />
Will an open source work ethic contribute to breaking the barriers of science that cordons off amateur, non scientific bio enthusiasts in art institutes, in hobby clubs, in public schools or on the street? This took us to places like Drishya and the National Institute of Design, the former, a school for the underprivileged in Bangalore and the latter, India’s first design research institute.<br />
</p><br />
<p><br />
Armed with scientific knowledge that we had gathered from peering over books, research papers, friends and guides undertaking advanced studies in biology and the internet, we set out to share what we had learnt through day long workshops with people as varied as seven year old kids, art professors, performance artists to students of advanced studies in Industrial design.<br />
</p><br />
<p><br />
We found success in the fact when people completely disconnected from the field related to the project and valued the importance of what we were trying to do. <br />
</p><br />
</div><br />
<br />
</div><br />
</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/TeamTeam:ArtScienceBangalore/Team2009-10-21T06:09:29Z<p>Yashas: </p>
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We consider ourselves amateurs/novices within the context of the IGEM competition. Our endeavor as “outsiders” is to bring our training in the arts and design to synthetic biology. Over this summer, we learnt the tools and techniques of synthetic biology and developed a piece of life which reflects our concerns, namely, the cultural, ethical and aesthetic implications of Synthetic Biology. Using a DIY approach and getting our hands “wet” was a critical element in the learning process. This approach allowed us to engage in constructive discussions with scientists working in the field.<br />
</html><br />
[[Image:Photo_61.jpg|right|frame|From left to right:Gautam, Nikhil, Sandeep, Dhruv, Avni, Sanya, Krupakar(the guy behind), Upasana and Akash Invisible: Neha]] <br />
<br />
[http://hackteria.org/wiki/index.php/User:Navadrian.cinthus Akash Hirosh]<br />
<br />
[http://hackteria.org/wiki/index.php/Avni_Sethi Avni Sethi]<br />
<br />
[http://hackteria.org/wiki/index.php/User:Iamnosuperman Dhruv Nawani]<br />
<br />
[http://hackteria.org/wiki/index.php/User:Gautamf472 Gautam Vishwanath]<br />
<br />
[http://hackteria.org/wiki/index.php/Krupakar_Dhinakaran Krupakar Dhinakaran]<br />
<br />
[http://hackteria.org/wiki/index.php/User:NehaBhat Neha Bhat]<br />
<br />
[http://hackteria.org/wiki/index.php/Nikhil_Patil Nikhil Patil]<br />
<br />
[http://hackteria.org/wiki/index.php/User:Sem2490 Sandeep Mathew]<br />
<br />
[http://hackteria.org/wiki/index.php/Sanya_Rai_Gupta Sanya Rai Gupta]<br />
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[http://hackteria.org/wiki/index.php/Upasana_Simha Upasana Simha]<br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangaloreTeam:ArtScienceBangalore2009-10-21T06:09:06Z<p>Yashas: </p>
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/AproachTeam:ArtScienceBangalore/Aproach2009-10-21T05:53:45Z<p>Yashas: </p>
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<p><br />
Focused on the ideology of making science accessible to the non-scientific community, we experimented with the Do-It-Yourself approach of working.<br />
</p><br />
<p><br />
We began with familiarizing ourselves with the nature of DIYBio and dipped our hands in beginner level scientific procedures that introduced us to the building blocks of the seemingly hurdle -filled stream that we were trying to pursue. From building our own gel electrophoresis chamber in our self constructed backyard bio lab in an Art and Design school campus to conducting gel electrophoresis in a professional lab, we played with the boundaries of the DIY style of working. A three time failure at an attempt to experiment with cell lysis followed and so did a deep insight into the skills of being a lab practitioner. We delved into process of science.<br />
</p><br />
<p><br />
Will an open source work ethic contribute to breaking the barriers of science that cordons off amateur, non scientific bio enthusiasts in art institutes, in hobby clubs, in public schools or on the street? This took us to places like Drishya and the National Institute of Design, the former, a school for the underprivileged in Bangalore and the latter, India’s first design research institute.<br />
</p><br />
<p><br />
Armed with scientific knowledge that we had gathered from peering over books, research papers, friends and guides undertaking advanced studies in biology and the internet, we set out to share what we had learnt through day long workshops with people as varied as seven year old kids, art professors, performance artists to students of advanced studies in Industrial design.<br />
</p><br />
<p><br />
We found success in the fact when people completely disconnected from the field related to the project and valued the importance of what we were trying to do. <br />
</p><br />
</div><br />
<br />
</div><br />
</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/GalleryTeam:ArtScienceBangalore/Gallery2009-10-21T05:53:10Z<p>Yashas: </p>
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/LinksTeam:ArtScienceBangalore/Links2009-10-21T05:52:22Z<p>Yashas: </p>
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<p><a href="http://srishti.ac.in/"> Srishti </a></p><br />
<p><a href="http://www.ncbs.res.in/index.php?option=com_content&task=view&id=349&Itemid=9"> NCBS </a></p><br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/OutreachTeam:ArtScienceBangalore/Outreach2009-10-21T05:50:57Z<p>Yashas: </p>
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/ProjectTeam:ArtScienceBangalore/Project2009-10-21T05:48:56Z<p>Yashas: </p>
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<p>Geosmin, which means ‘earth odor’, is a volatile microbial metabolite that is responsible for the characteristic smell of moist soil or freshly plowed earth. Geosmin is produced by a number of microorganisms, including most Streptomyces and several species of cyanobacteria, myxobacteria and fungi. Besides its pleasant, characteristic earthy aroma, geosmin is also associated with an undesirable musty odor or off flavor in drinking water, as well as in wine, fish, and other food stuffs. The structure of geosmin was first established as trans- 1,10-dimethyl-trans-9 decalol by N.N Gerber who detected the volatile oil in 17 different species of Streptomyces and a blue green alga following its initial isolation from S. griseus. </p><br />
<br />
<p>Biosynthesis of geosmin from farnesyl diphosphate is catalyzed by a single enzyme germacradienol/germacrene D synthase. Escherichia coli: ispA codes for farnesyl diphosphate (FPP) synthase. FPP synthase catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (C5) and geranyl diphosphate (C10) to form FPP. </p><br />
<br />
<p>E. coli, does not bear a gene that codes for germacradienol/germacrene D synthase. In our project, we are expressing germacradienol/germacrene D synthase gene in different strains of E. coli, under control of pTrc promoter present in pTrc99a vector (BBa_”---“). This is basically an expression vector (that contains a LacI repressor) which can be induced by IPTG. A map of this vector is given below :</p><br />
<br />
<p>This synthase plasmid SF5 ( ispA+, triangle (srl-recA) 306::Tn10) encoding the whole protein is transformed to the full-length wild strain SF5 to produce Geosmin and eventually the ‘smell’ of rain. This is tested against a knockout strain SF7N ( triangle ispA::neo, triangle (srl-recA) 306 :: Tn10) that did not contain farnesyl synthase which is the precursor to the production of farnesyl diphosphate. Hence, when the plasmid is inoculated, no Geosmin will be expected to form.</p><br />
<br />
<h3>Experimental protocol:</h3><br />
<ol><br />
<li>E. coli k12z1 bearing BBa_”---“and k12z1 was grown overnight at 37oC and 250rpm in Luria Bertani (LB) medium containing 100uM ampicillin and spectinomycin respectively.</li><br />
<li>BBa_”---“culture was inoculated in fresh 1X glucose-M9 medium, containing 0, 50, 500 and 1000uM of IPTG. AS a negative control K12z1 was inoculated in fresh 1X glucose-M9 medium.</li><br />
<li>The culture was grown for 12h at 37oC and 250rpm.</li><br />
<li>Geosmin analysis was done through smell, and it appeared that k12z1 cells bearing BBa_”--“, are geosmin positive.</li><br />
</ol><br />
<br />
<p>We have managed to obtain a mutated strain pSF27 (ispA+, cm R,pSC 101 Ori ts) from Dr. XX at Toho University, which is temperature- sensitive and using this, a controlled ispA strain is being constructed by transforming SF7N with pSF27 wherein on reducing the temperatures, synthesis of Geosmin takes place and the smell of rain is produced.<br />
</p><br />
<h3>What did and did not work</h3><br />
<p>While working with the full length synthase plasmid SF5, and testing it with the knockout strain SF7N, no difference could be observed in the two resultant smells. From this we inferred that geosmin was not being synthesized as per our theoretical analysis.</p><br />
<ul><br />
<li>One reason for this could be the fact that the concentrations of farnesyl diphosphate may not be accurate and require further trial and error through experimentation.</li><br />
<li>It was also discovered that the C- terminal of the synthase contained a restriction enzyme which could well be the reason for the failure of our experiment. We also discovered that the expression of the N-terminal domain of the plasmid pTRC99a gave a fully functional germacradienol synthase with steady-state catalytic parameters similar to those of the full-length protein but the expressed C-terminal domain had no detectable FPP cyclase activity. Hence, it was only the N- terminal that was needed for the synthesis of geosmin and the C- terminal was the half that contained the restriction enzyme and as it played no part in the synthesis process, it could be deleted from the gene.</li><br />
</ul><br />
<br />
<img src="https://static.igem.org/mediawiki/2009/0/09/Geosmin_reaction.jpg" width="500px"></img><br />
<br />
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</html></div>Yashashttp://2009.igem.org/Team:ArtScienceBangalore/AproachTeam:ArtScienceBangalore/Aproach2009-10-21T05:46:40Z<p>Yashas: </p>
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<p><br />
Focused on the ideology of making science accessible to the non-scientific community, we experimented with the Do-It-Yourself approach of working.<br />
</p><br />
<p><br />
We began with familiarizing ourselves with the nature of DIYBio and dipped our hands in beginner level scientific procedures that introduced us to the building blocks of the seemingly hurdle -filled stream that we were trying to pursue. From building our own gel electrophoresis chamber in our self constructed backyard bio lab in an Art and Design school campus to conducting gel electrophoresis in a professional lab, we played with the boundaries of the DIY style of working. A three time failure at an attempt to experiment with cell lysis followed and so did a deep insight into the skills of being a lab practitioner. We delved into process of science.<br />
</p><br />
<p><br />
Will an open source work ethic contribute to breaking the barriers of science that cordons off amateur, non scientific bio enthusiasts in art institutes, in hobby clubs, in public schools or on the street? This took us to places like Drishya and the National Institute of Design, the former, a school for the underprivileged in Bangalore and the latter, India’s first design research institute.<br />
</p><br />
<p><br />
Armed with scientific knowledge that we had gathered from peering over books, research papers, friends and guides undertaking advanced studies in biology and the internet, we set out to share what we had learnt through day long workshops with people as varied as seven year old kids, art professors, performance artists to students of advanced studies in Industrial design.<br />
</p><br />
<p><br />
We found success in the fact when people completely disconnected from the field related to the project and valued the importance of what we were trying to do. <br />
</p><br />
</div><br />
<br />
</div><br />
</html></div>Yashas