Search results

From 2009.igem.org

Create the page "Cadmium" on this wiki!

Page title matches

  • Team:Newcastle/Project/Cadmium
    #REDIRECT[[Team:Newcastle/Cadmium]]
    35 B (4 words) - 13:24, 3 August 2009
  • Team:Newcastle/Cadmium sensing
    ==Cadmium Sensing== ...ranscription to occur. These proteins therefore allow selective sensing of cadmium in the form of an AND gate, a model for which can be seen in our modelling
    711 B (106 words) - 09:39, 22 July 2009
  • Team:Newcastle/Cadmium
    =Why cadmium?= The BacMan project is focused mainly on sequestering the heavy metal cadmium from contaminated soils, however with the many other heavy metals known to
    4 KB (684 words) - 12:59, 31 July 2009

Page text matches

  • Team:Newcastle/Project
    ...rporated into a ''Bacillus'' spore, the resilience of which means that the cadmium ions can become isolated from the environment (and made bio-unavailable) fo # [https://2009.igem.org/Team:Newcastle/Project#Cadmium_Intake Cadmium Intake]
    16 KB (2,501 words) - 02:05, 22 October 2009
  • Team:Newcastle/Choices
    ==Why deal with cadmium?== The BacMan project is focused mainly on sequestering the heavy metal cadmium from contaminated soils, however with the many other heavy metals known to
    6 KB (890 words) - 20:26, 21 October 2009
  • Team:Newcastle/Metalsensing
    This sub-project focusses on getting the ''Bacillus subtilis'' to sense the cadmium and being able to respond to this detection. Please click on the links belo ... sensors ensures the ''Bacillus subtilis'' cells have a tightly controlled cadmium sensor, as naturally occurring metal sensitive promoters can respond to OTH
    10 KB (1,512 words) - 02:54, 22 October 2009
  • Team:Cornell/Project
    ==Cadmium Contamination== ...ubstances and Disease Registry). For instance, in late 20th century Japan, cadmium contamination of water in the Jingzu River led to significant kidney proble
    8 KB (1,172 words) - 03:50, 22 October 2009
  • Team:Newcastle/Cadmium
    =Why cadmium?= The BacMan project is focused mainly on sequestering the heavy metal cadmium from contaminated soils, however with the many other heavy metals known to
    4 KB (684 words) - 12:59, 31 July 2009
  • Team:Cornell/Project/Background
    ==Cadmium Contamination== ...age:Itai_itai.png|right|frame|Patient suffering from prolonged exposure to cadmium]]
    5 KB (722 words) - 03:53, 22 October 2009
  • Jamboree/Project Abstract/Team Abstracts
    ...gineering the Bacillus Subtilis Metal Ion Homeostasis System to Serve as a Cadmium Responsive Biosensor=== ... bacterial biosensors and are unable to measure the amount of bioavailable cadmium.
    105 KB (15,465 words) - 15:13, 5 November 2009
  • Team:Newcastle
    '''Cadmium contamination''' can be a serious problem in countries where polluting indu ...ble'''. Germination of the spores can be disabled, making retrieval of the cadmium unnecessary since they can persist intact for thousands of years.
    9 KB (1,304 words) - 11:37, 9 November 2009
  • Team:Newcastle/Modeling/Population/Pseudo/Bacteria Code
    * importCadmium - import cadmium from the environment (MntH) * exportCadmium - export cadmium to the environment (CadA)
    4 KB (704 words) - 02:44, 22 October 2009
  • Team:Newcastle/Stochasticity
    ** Decreased cadmium efflux ** Upregulation of cadmium import
    11 KB (1,565 words) - 02:23, 22 October 2009
  • Team:Newcastle/Chassis
    ...o the spores of our engineered ''B. subtilis'', but what happens after the cadmium has been sequestered? ...to retrieve the sequestered cadmium? Or do we simply leave the sequestered cadmium in the spores of our engineered ''B. subtilis''?
    7 KB (998 words) - 22:15, 21 October 2009
  • Team:Newcastle/Labwork/16 September 2009
    ...scovered that the attempts to transform ''E. coli'' with the ''arsR-cadA'' cadmium sensor failed, so on that same day we carried out a second attempt at trans '''The plate containing the cadmium-sensor ''E. coli'' transformants will also be studied - if there are coloni
    10 KB (1,524 words) - 12:17, 20 August 2010
  • Team:Newcastle/Chassis/Introduction
    ...o the spores of our engineered ''B. subtilis'', but what happens after the cadmium has been sequestered? ...o retrieve the sequestered cadmium? Or, do we simply leave the sequestered cadmium in the spores of our engineered ''B. subtilis''?
    4 KB (703 words) - 22:16, 21 October 2009
  • Team:Cornell/Project/Design
    ...nds and represses the cadA regulatory region and is released when bound by cadmium ions. [7] ==Cadmium Sensor Module 1==
    4 KB (523 words) - 03:55, 22 October 2009
  • Team:Newcastle/Meetings/2 July 2009
    ** It was discussed that we first of all need to model cadmium only and THEN we can consider other metals - start from the basics. ** Need to find out how much cadmium binds to the promoter/protein per second
    6 KB (957 words) - 11:50, 9 July 2009
  • Team:Newcastle/Safety
    ...e did not get to this point. The UK Government's guidance for working with cadmium are at: [http://www.hse.gov.uk/pubns/indg391.pdf HSE's guidance]. ...hrough the human alimentary canal. However, there may be a small risk from cadmium bound to the surface of the spore, which may leach from metallathionin-''co
    5 KB (744 words) - 01:02, 22 October 2009
  • Team:KU Seoul/Notebook
    ...vy metals which are harmful for environment, such as arsenic, mercury, and cadmium. The purpose of this project is to detect the concentration of heavy metals It's a cadmium specific protein.
    33 KB (5,505 words) - 17:15, 21 October 2009
  • Team:Newcastle/Metals
    ...e coat protein - this means that, should the stochastic switch decide, any cadmium mopped up could be sequestered into spores thereby making it unavailable. P In order to take up and keep the cadmium from the soil efficiently, we needed to think about a way to cross-link the
    10 KB (1,556 words) - 03:56, 22 October 2009
  • Team:Cornell/Project/Chassis
    ...ncentration of heavy metals we grew B subtilis in various concentration of Cadmium and compared growth curves. ...tein that Cadmium influxes through increases B subtilis ability to grow in Cadmium.
    2 KB (340 words) - 03:59, 22 October 2009
  • Team:Newcastle/Meetings/29 June 2009
    *** It was discussed that we first of all need to model cadmium only and THEN we can consider other metals - start from the basics. *** Need to find out how much cadmium binds to the promoter/protein per second
    4 KB (703 words) - 11:48, 9 July 2009
  • Team:Newcastle/Cadmium sensing
    ==Cadmium Sensing== ...ranscription to occur. These proteins therefore allow selective sensing of cadmium in the form of an AND gate, a model for which can be seen in our modelling
    711 B (106 words) - 09:39, 22 July 2009
  • Team:Newcastle/Metalintakeefflux
    ...e it would be logical to find a way in which we can increase the intake of cadmium without increasing the intake of other metals too. It is known that a ''Bacillus subtilis'' cell takes up cadmium naturally through the manganese transport system.
    2 KB (289 words) - 03:16, 22 October 2009
  • Team:Cornell/Notebook
    ...Cadmium and Zinc Beta Galactosidase Assay-- To characterize the effects of Cadmium and Zinc concentration on the promoter for the CadA-lacZ fusion. ...Cadmium and Zinc Beta Galactosidase Assay-- To characterize the effects of Cadmium and Zinc concentration on the promoter for the CadA-lacZ fusion.
    25 KB (3,891 words) - 03:58, 22 October 2009
  • Team:Newcastle/Meetings/23 March 2009
    ** Matt - sensing cadmium (CzrA, CueR, ArsR). *** Cerevicie (sp?), Crs5 – zinc, cadmium.
    5 KB (678 words) - 12:39, 16 June 2009
  • Team:Cornell/Parts
    ''Cadmium Sensor (BBa_K285104)'' ... for Bacillus Subtilis. The GFP coding sequence follows. Note that the GFP Cadmium Sensor will only function with the czrA regulon found in Bacillus Subtillis
    2 KB (361 words) - 03:58, 22 October 2009
  • Team:Groningen/Project/Accumulation
    ...a variety of heavy metals as they bind to a wide range of metals including cadmium, zinc, mercury, copper, arsenic, silver, coordinated in metal-thiolates. Th ...r different metals has been investigated on several occasions, such as for cadmium ([[Team:Groningen/Literature#Deng2007|Deng 2007]]), arsenic ([[Team:Groning
    35 KB (5,337 words) - 22:10, 21 October 2009
  • Team:Newcastle/Modeling/Population
    ...er sporulation. Spores that cannot germinate because they have sequestered cadmium are shown as red spheres. Germinating spores (that have previously been non
    8 KB (1,197 words) - 02:51, 22 October 2009
  • Team:Newcastle/Judging Comments
    ...tional repressors is a tightly regulated promoter which can be used by any cadmium-sensitive system. ... the behaviour of soil bacterium is a worthy aim and the idea of packaging cadmium in spores is unique.
    3 KB (422 words) - 01:23, 22 October 2009
  • Team:KU Seoul/Parts
    ...d of stationary period. Promoter yodA is only transiently activated during cadmium-induced growth inhibition.
    2 KB (227 words) - 15:51, 21 October 2009
  • Team:Newcastle/Meetings/22 June 2009
    ** locate cadmium in spore ** show the bacterial will turn green when sense cadmium
    719 B (99 words) - 11:47, 9 July 2009
  • Team:Newcastle/SporulationTuning
    ...wcastle/Metalintakeefflux ‘metal container’], trapping the sequestered cadmium in its spore. ...esters cadmium into its spore, it should not germinate, or the sequestered cadmium will be released back into the environment. Therefore, the [https://2009.ig
    8 KB (1,197 words) - 02:11, 22 October 2009
  • Team:Newcastle/Metal Sensor planB
    ...sses based on the action of metal sensors. These metal sensors will detect cadmium through a system known as AND Gating. There are two metal sensing repressors, which are known to respond to cadmium: arsR and czrA.
    2 KB (349 words) - 03:20, 22 October 2009
  • Team:Newcastle/Labwork/27 August 2009
    ...pt at digesting the ''BBa_J33206'' BioBrick; also made a real start on the cadmium-sensor AND gate by digesting ''BBa_J33206'' in ''pSB1A2'', consequently rem ...ell as this the team needs to begin acting on the cloning strategy for the cadmium-sensing AND gate (i.e. placing the ''cadA'' promoter region next to the ''a
    11 KB (1,900 words) - 12:08, 20 August 2010
  • Team:Newcastle/SporulationTuning/Introduction
    ...wcastle/Metalintakeefflux ‘metal container’], trapping the sequestered cadmium in its spore. ...uesters cadmium into its spore, it should not germinate or the sequestered cadmium will be released back into the environment as a result. Therefore, the role
    7 KB (1,106 words) - 02:24, 22 October 2009
  • Team:Newcastle/Meetings/11 June 2009
    * Write up on Cadmium and the environment (Ongoing, part complete) ** Why do we want to clean up Cadmium from the environment? Showed the prepared draft document - "All" wording to
    4 KB (646 words) - 12:40, 16 June 2009
  • Team:Newcastle/Meetings/23 July 2009
    *Write up about Cadmium and the environment - ongoing (Jess, Mathew) *Write up on the use of Cadmium in the lab (ongoing)
    5 KB (813 words) - 12:09, 30 July 2009
  • Team:Newcastle/Meetings/9 July 2009
    * <b>Write up about Cadmium and the Environment</b> (Jess, Mathew)(ongoing) * Write about the use of cadmium in the lab (safety issues)
    2 KB (329 words) - 11:34, 15 July 2009
  • Team:Newcastle/Meetings/16 July 2009
    *Write up about Cadmium and the environment - ongoing (Jess, Mathew) *Write up on the use of Cadmium in the lab (ongoing)
    3 KB (518 words) - 16:14, 16 July 2009
  • Team:Newcastle/Site map
    *** [[Team:Newcastle/Project/Cadmium]] ** [[Team:Newcastle/Cadmium]]
    944 B (104 words) - 10:42, 16 October 2009
  • Team:Newcastle/Modelling/MetalIntake
    <b>The Cadmium Intake Model is available for download as follows:</b> * Text Format: [[Media:TeamNewcastleCadmiumUptakeModel.txt| Cadmium Intake Model]]
    1 KB (155 words) - 02:48, 22 October 2009
  • Team:Newcastle/Labwork/14 September 2009
    ...ad previously carried out a PCR reaction involving the two elements of our cadmium-sensitive logic AND gate - ''arsR'' and ''cadA''. But when the products wer ====1) Cadmium-sensing logic AND gate====
    11 KB (1,625 words) - 12:16, 20 August 2010
  • Team:Newcastle/Modeling/Population/Pseudo
    * Cell - contains the common attributes, such as levels of cadmium and nutrients. The Cell class also provides access to the database. ...tion. This includes what state it is in at the moment (eg spore), how much cadmium is contained within, if it can germinate, etc.
    5 KB (811 words) - 03:01, 22 October 2009
  • Team:Groningen/Literature
    :Deng X, Yi XE, Liu G. (2007). "[http://www.ncbi.nlm.nih.gov/pubmed/16890348 Cadmium removal from aqueous solution by gene-modified Escherichia coli JM109]". ''
    36 KB (4,612 words) - 23:47, 21 October 2009
  • Team:Groningen/Project/Transport
    ... (lead), CueR (copper), ZntR (zinc), MerR (mercury), ArsR (arsenic), CadR (cadmium)).
    17 KB (2,607 words) - 23:16, 21 October 2009
  • Team:Newcastle/Meetings/11 May 2009
    *Write up on Cadmium and the environment
    2 KB (314 words) - 12:25, 16 June 2009
  • Team:Newcastle/Meetings/16 March 2009
    *** Cadmium, Mercury, Arsenic >> Red List metals
    4 KB (583 words) - 21:48, 2 July 2009
  • Team:Newcastle/Meetings/16 June 2009
    * Write up on Cadmium and the environment (Ongoing, part complete)
    2 KB (261 words) - 12:40, 16 June 2009
  • Team:Newcastle/Meetings/18 June 2009
    * Write up on Cadmium and the environment (Ongoing, part complete)
    2 KB (296 words) - 11:45, 9 July 2009
  • Team:Newcastle/Project/Cadmium
    #REDIRECT[[Team:Newcastle/Cadmium]]
    35 B (4 words) - 13:24, 3 August 2009
  • Team:Newcastle/Modelling
    Another of our models looks at metal intake. Specifically this looks at Cadmium transport throughout our bacteria.
    2 KB (328 words) - 20:58, 21 October 2009
  • Team:Newcastle/Meetings/8 October 2009
    ...ilis' cell on the front page of the wiki with elements of the project e.g. Cadmium sensing, stochastic switch, etc included
    5 KB (805 words) - 12:57, 9 October 2009
  • Team:KU Seoul/Project
    ...sor to detect three major hamful metals such as arsenic, mercury, lead and cadmium utilizing the law of light mixing among three colors "green, red, blue".
    2 KB (255 words) - 01:30, 14 October 2009
  • Team:Newcastle/Modeling/Stochastic
    ...d in our system to a give a decision to be a metal container and sequester cadmium, or continue to normal vegetative life. This cell fate decision is given ba
    6 KB (858 words) - 23:47, 21 October 2009
  • Team:KU Seoul/Overview
    :arsenic, zinc, and cadmium in E. coli. The ultimate goal is to build a micromachine sensing and determ
    2 KB (254 words) - 14:12, 21 October 2009
  • Team:Newcastle/Meetings/3 September 2009
    ** Ask Nigel Robinson about working with Cadmium, and an electromicroscope.
    4 KB (667 words) - 10:35, 4 September 2009
  • Team:Newcastle/Meetings/10 September 2009
    * Ask Nigel Robinson about working with Cadmium, and an electromicroscope.
    3 KB (518 words) - 16:15, 11 September 2009
  • Team:Newcastle/Labwork/11 September 2009
    ...us the promoter region) and ''cadA'' promoter region - these will form the cadmium-sensing AND gate. The plan is to run these products on a gel and if we get
    20 KB (2,992 words) - 12:15, 20 August 2010
  • Team:Newcastle/Labwork/15 September 2009
    ... ligations and transformations were a success. This cannot be said for the cadmium sensor 'transformant' cells which did not yield any colonies on the LB + am
    7 KB (1,112 words) - 12:17, 20 August 2010
  • Team:Newcastle/Labwork/17 September 2009
    ... promoter cut out) but only got as far as digesting the two fragments. The cadmium-sensor has been put on hold as of the [https://2009.igem.org/Team:Newcastle
    11 KB (1,594 words) - 14:09, 21 October 2009
  • Team:Newcastle/Meetings/30 September 2009
    ...is''' cell on the front page of the wiki with elements of the project e.g. Cadmium sensing, stochastic switch, etc included
    4 KB (689 words) - 10:41, 8 October 2009
  • Team:Newcastle/Meetings/15 October 2009
    ...ilis' cell on the front page of the wiki with elements of the project e.g. Cadmium sensing, stochastic switch, etc included
    2 KB (369 words) - 19:47, 19 October 2009
  • Team:Newcastle/Meetings/5 October 2009
    *** Could use/affect amounts of nutrients/cadmium, change the rates of import and export, change the chance of sequestering,
    3 KB (403 words) - 12:09, 8 October 2009
  • Team:Newcastle/Games/SirBacMan
    [[Team:Newcastle/Project/Cadmium|Poison]] is the ancient currency – not ours. Let us expunge these vile de
    2 KB (316 words) - 17:32, 21 October 2009
  • Team:Groningen/Parts/Submitted Parts
    ...allothionein it is also suitable to bind other metals like zinc, copper or cadmium. This biobrick already contains the ribosomal binding site <partinfo>B0034<
    11 KB (1,733 words) - 21:34, 21 October 2009
  • Team:Newcastle/Meetings/12 October 2009
    ...ilis' cell on the front page of the wiki with elements of the project e.g. Cadmium sensing, stochastic switch, etc included
    3 KB (432 words) - 22:15, 11 October 2009
  • Team:KU Seoul/Result
    ...similar problem related to toxicity, as discussed above. Like arsenic ion, cadmium has
    7 KB (908 words) - 16:42, 21 October 2009
  • Team:Newcastle/Meetings/20 October 2009
    ...ilis' cell on the front page of the wiki with elements of the project e.g. Cadmium sensing, stochastic switch, etc included
    1 KB (153 words) - 10:29, 21 October 2009
  • Team:Valencia/A short story/Osamu
    ...medicine. GFP has been used also in other fields, such as the detection of cadmium, zinc, and explosive TNT and fumes (Zimmer, 2005). The range of application
    34 KB (5,690 words) - 07:03, 20 October 2009

View (previous 500 | next 500) (20 | 50 | 100 | 250 | 500)