http://2009.igem.org/wiki/index.php?title=Talk:Team:Waterloo/Notebook/Oligos&feed=atom&action=history
Talk:Team:Waterloo/Notebook/Oligos - Revision history
2024-03-28T11:21:15Z
Revision history for this page on the wiki
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Hparikh: New page: {| width="100%" border="1" cellpadding="2" ! Description !! Oligo Name !! Sequence !! Length |- |BioBrick sequencing/colPCR primer (fwd) || iGEMvf2 || TGCCACCTGACGTCTAAGAA || 20 |- |BioBr...
2009-10-20T05:08:55Z
<p>New page: {| width="100%" border="1" cellpadding="2" ! Description !! Oligo Name !! Sequence !! Length |- |BioBrick sequencing/colPCR primer (fwd) || iGEMvf2 || TGCCACCTGACGTCTAAGAA || 20 |- |BioBr...</p>
<p><b>New page</b></p><div>{| width="100%" border="1" cellpadding="2"<br />
! Description !! Oligo Name !! Sequence !! Length<br />
|-<br />
|BioBrick sequencing/colPCR primer (fwd) || iGEMvf2 || TGCCACCTGACGTCTAAGAA || 20<br />
|-<br />
|BioBrick sequencing/colPCR primer (rev) || iGEMvr || ATTACCGCCTTTGAGTGAGC || 20<br />
|-<br />
|Primer for Lac promoter (fwd) || PLacF || TTATGCTTCCGGCTCGTATG || 20<br />
|-<br />
|Primer for {BioBrick Prefix}-{B0034} (rev; for use with iGEMvf2)|| iGEMpre+rbs || TTTCTCCTCTTTCTCTAGATGCGG || 24<br />
|-<br />
|Primer for {BioBrick Prefix}-{B0034} (fwd; for use with iGEMvr) || PrefixRBS-F || GGCCGCTTCTAGAGAAAGAGG || 21<br />
|-<br />
|Gene 5 ORF from T7 phage (fwd; Prefix adapter) || T7gene5-F || CAATGGAATTCGCGGCCGCTTCTAGATGATCGTTTCTGACATCGAA || 46<br />
|-<br />
|Gene 5 ORF from T7 phage (rev; Suffix adapter) || T7gene5-R || CAATTTCTGCAGCGGCCGCTACTAGTATCAGTGGCAAATCGCCCAATTAGG || 51<br />
|-<br />
|Gene 6 ORF from T7 phage (fwd; Prefix adapter) || T7gene6-F || CAATGGAATTCGCGGCCGCTTCTAGATGGCACTTCTTGACCTTAAA || 46<br />
|-<br />
|Gene 6 ORF from T7 phage (rev; Suffix adapter) || T7gene6-R || CAATTTCTGCAGCGGCCGCTACTAGTACTACGGTCTCCACAGG || 43<br />
|-<br />
|PmeIR ORF (fwd) || PmeIR-F || ATCCGGAATTCGCGGCGCTTTCTAGATGACAACAAACTCCCCCTCAGACG || 50<br />
|-<br />
|PmeIR ORF (rev) || PmelR-R || CGCAAAACTGCAGCGGCCGCTACTAGTATCAAACCGTACTTCGCCCCCGTTG || 52<br />
|-<br />
|pRecA oligo (sense strand; produces BioBrick-compatible EcoRI/SpeI overhangs when annealed to recAR) || recAF || AATTCGCGGCCGCTTCTAGAGTACAAAACACTTGATACTGTATATATATACAGTATAATTGCTTCAACATA || 71<br />
|-<br />
|pRecA oligo (antisense strand; produces BioBrick-compatible EcoRI/SpeI overhangs when annealed to recAR) || recAR || CTAGTATGTTGAAGCAATTATACTGTATATATATACAGTATCAAGTGTTTTGTACTCTAGAAGCGGCCGCG || 71<br />
|-<br />
|{B0034}-{T7gene6 ORF} (fwd; for adding {Prefix-RBS} onto the gene 6 ORF by PCR) || RBST7g6-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCACTTCTTGACCTTAAAC || 61<br />
|-<br />
|T7gene6 ORF (rev) || T7gs-R || CTGCAGCGGCCGCTACTAGTATTATTACGGTCTCCACAGGTAAATCTC || 48<br />
|-<br />
|T7gene6 ORF rev; for joining to PmeI with crossover PCR || T7g6-RXO-RBS || CTAGTATTTCTCCTCTTTCTCTAGTATTATTACGGTCTCCACAGGTAAATCTC || 53<br />
|-<br />
|PmeI ORF (fwd; for adding prefix-RBS) || RBSpmeI-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG || 61<br />
|-<br />
|PmeI ORF (fwd; for adding prefix-RBS and joining to T7gene6 by crossover PCR) || RBSpmeI-FXO || TACTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG || 48<br />
|-<br />
|PmeI ORF (rev; TAATAA stop codon-suffix) || pmeI-R || CTGCAGCGGCCGCTACTAGTATTATTAAACCGTACTTCGCCCCCG || 45<br />
|-<br />
|Primer for part R0011 (Plac-PL hybrid promoter; fwd) || R0011-F || GAATTCCGGCCGCTTCTAGAGAATTGTGAGCGGATAAC || 38<br />
|-<br />
|Primer for part R0011 (Plac-PL hybrid promoter; rev for joining to ORFs with RBS by crossover PCR)|| R0011RBS-RXO || CTAGTATTTCTCCTCTTTCTCTAGTATGTGCTCAGTATCTTGTTATC || 47<br />
|-<br />
|CI ORF (fwd; prefix-RBS) || RBSC0051-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC || 59<br />
|-<br />
|CI ORF (fwd; prefix-RBS for joining to R0011 promoter by crossover PCR) || RBSC0051-FXO || TACTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC || 46<br />
|-<br />
|CI ORF (rev; suffix adapter) || C0051-R || CTGCAGCGGCCGCTACTAGTATTATTAAGCTACTAAAGCG || 40<br />
|-<br />
|CI ORF (rev; for joining to transcriptional terminator (b0015) by crossover PCR) || C0051-RXO || GATGCCTGGCTCTAGTATTATTAAGCTACTAAAGCG || 36<br />
|-<br />
|Transcriptional terminator (B0015; fwd; for joining to ORFs by crossover PCR) || B0015-FXO || TAATAATACTAGAGCCAGGCATCAAATAAAACG || 33<br />
|-<br />
|Transcriptional terminator (B0015; rev) || B0015-R || CTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCC || 39<br />
|-<br />
|RFP ORF (fwd; prefix-RBS) || RFP-RBS-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG || 58<br />
|-<br />
|RFP ORF (fwd; prefix-RBS for joining to transcriptional terminator by crossover PCR ) || RFP-RXO || GATGCCTGGCTCTAGTATTATTAAGCACCGGTGG || 34<br />
|-<br />
|Plac (r0011; fwd; for adding lac promoter and RBS to ORFs by PCR) || Plac-RBS-F || GAATTCGCGGCCGCTTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCAC ATACTAGAGAAAGAGGAGAAATACTAG || 103<br />
|}<br />
<br></div>
Hparikh