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Team:Alberta/Project/Primer Design
From 2009.igem.org
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<P>To assemble the minimal genome, essential genes are first PCR amplified from genomic DNA and cloned into universal plasmids described in the bead assembly section. By including restriction ennzyme cut sites on the primers, PCR products can be digested and ligated directly into the plasmid. </P> | <P>To assemble the minimal genome, essential genes are first PCR amplified from genomic DNA and cloned into universal plasmids described in the bead assembly section. By including restriction ennzyme cut sites on the primers, PCR products can be digested and ligated directly into the plasmid. </P> | ||
Revision as of 15:02, 18 September 2009
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Primer DesignTo assemble the minimal genome, essential genes are first PCR amplified from genomic DNA and cloned into universal plasmids described in the bead assembly section. By including restriction ennzyme cut sites on the primers, PCR products can be digested and ligated directly into the plasmid. Method for Primer DesignWe've developed a streamlined method for primer design. Using this method, we've designed over 300 primers. Using these primers, over 150 essential genes have already been amplified. |
