Team:Alberta/Project/Primer Design

From 2009.igem.org

(Difference between revisions)
Line 36: Line 36:
<P>To assemble the minimal genome, essential genes are first PCR amplified from genomic DNA and cloned into universal plasmids described in the DNA assembly section. By including restriction ennzyme cut sites on the primers, PCR products can be digested and ligated directly into the plasmid. </P>
<P>To assemble the minimal genome, essential genes are first PCR amplified from genomic DNA and cloned into universal plasmids described in the DNA assembly section. By including restriction ennzyme cut sites on the primers, PCR products can be digested and ligated directly into the plasmid. </P>
-
<h3>Method for Primer Design</h3>
+
<p> We've designed over 300 primers for essential genes. Using these primers, over 150 essential genes have already been amplified. </p>
-
<p>We've developed a streamlined method for primer design. Using this method, we've designed over 300 primers. Using these primers, over 150 essential genes have already been amplified. </p>
+
</font></div>
</font></div>

Revision as of 19:01, 18 September 2009

University of Alberta - BioBytes










































































































Primer Design

To assemble the minimal genome, essential genes are first PCR amplified from genomic DNA and cloned into universal plasmids described in the DNA assembly section. By including restriction ennzyme cut sites on the primers, PCR products can be digested and ligated directly into the plasmid.

We've designed over 300 primers for essential genes. Using these primers, over 150 essential genes have already been amplified.

Retrieved from "http://2009.igem.org/Team:Alberta/Project/Primer_Design"