"
Team:Alberta/References/Publications/A microfluidic mammalian cell sorter based onfluorescence detection
From 2009.igem.org
(New page: Authors: V. Studer, , R. Jameson, E. Pellereau, A. Pépin and Y. Chen Microelectronic Engineering, Volumes 73-74, June 2004, Pages 852-857 <b>Abstract:</b> We report on the development ...) |
|||
| Line 5: | Line 5: | ||
<b>Abstract:</b> We report on the development of microfluidic devices for single mammalian cell sorting and manipulation. These microfluidic devices are fabricated out of polydimethylsiloxane (PDMS) by multilayer soft lithography. They consist of several active units (mixer, pumps) pneumatically actuated by monolithic soft microvalves. Using this fabrication method we were able to develop a microfluidic device for the fast sorting of 10 μm diameter fluorescently tagged rare objects (mammalian cells or beads) sparsely distributed within a concentrated solution of non-tagged objects. We show that once sorted, these objects can be individually recovered in a small volume (nanolitre range) for further biochemical assays such as cell lysis, mRNA extraction and polymerase chain reaction. | <b>Abstract:</b> We report on the development of microfluidic devices for single mammalian cell sorting and manipulation. These microfluidic devices are fabricated out of polydimethylsiloxane (PDMS) by multilayer soft lithography. They consist of several active units (mixer, pumps) pneumatically actuated by monolithic soft microvalves. Using this fabrication method we were able to develop a microfluidic device for the fast sorting of 10 μm diameter fluorescently tagged rare objects (mammalian cells or beads) sparsely distributed within a concentrated solution of non-tagged objects. We show that once sorted, these objects can be individually recovered in a small volume (nanolitre range) for further biochemical assays such as cell lysis, mRNA extraction and polymerase chain reaction. | ||
| - | '''Link:''' [ http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6V0W-4C62R1D-3&_user=1067472&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1058511935&_rerunOrigin=scholar.google&_acct=C000051251&_version=1&_urlVersion=0&_userid=1067472&md5=b6052cab819e7688d099b5b53e49b586 Science Direct] | + | '''Link:''' [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6V0W-4C62R1D-3&_user=1067472&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1058511935&_rerunOrigin=scholar.google&_acct=C000051251&_version=1&_urlVersion=0&_userid=1067472&md5=b6052cab819e7688d099b5b53e49b586 Science Direct] |
Latest revision as of 22:13, 21 October 2009
Authors: V. Studer, , R. Jameson, E. Pellereau, A. Pépin and Y. Chen
Microelectronic Engineering, Volumes 73-74, June 2004, Pages 852-857
Abstract: We report on the development of microfluidic devices for single mammalian cell sorting and manipulation. These microfluidic devices are fabricated out of polydimethylsiloxane (PDMS) by multilayer soft lithography. They consist of several active units (mixer, pumps) pneumatically actuated by monolithic soft microvalves. Using this fabrication method we were able to develop a microfluidic device for the fast sorting of 10 μm diameter fluorescently tagged rare objects (mammalian cells or beads) sparsely distributed within a concentrated solution of non-tagged objects. We show that once sorted, these objects can be individually recovered in a small volume (nanolitre range) for further biochemical assays such as cell lysis, mRNA extraction and polymerase chain reaction.
Link: Science Direct
