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Team:Alberta/References/Publications/Accurate multiplex gene synthesisfrom programmable DNA microchips
From 2009.igem.org
Authors: Tian, JD., Gong, H., Sheng, NJ., Zhou, XC., Gulari, E., Gao, XL., Church, G
Nature 432(7020): 1050-1054. Dec 2004
Abstract: Testing the many hypotheses from genomics and systems biology experiments demands accurate and cost-effective gene and genome synthesis. Here we describe a microchip-based technology for multiplex gene synthesis. Pools of thousands of 'construction' oligonucleotides and tagged complementary 'selection' oligonucleotides are synthesized on photo-programmable microfluidic chips(1), released, amplified and selected by hybridization to reduce synthesis errors ninefold. A one-step polymerase assembly multiplexing reaction assembles these into multiple genes. This technology enabled us to synthesize all 21 genes that encode the proteins of the Escherichia coli 30S ribosomal subunit, and to optimize their translation efficiency in vitro through alteration of codon bias. This is a significant step towards the synthesis of ribosomes in vitro and should have utility for synthetic biology in general.
Link: Deep Blue
