Team:ArtScienceBangalore/Notebook/Week Four

From 2009.igem.org

(Difference between revisions)
(June 9th)
(June 9th)
Line 55: Line 55:
===June 9th===
===June 9th===
-
[[http://hackteria.org/wiki/index.php/The_June_9th_Image_Gallery The June 9th Image Gallery]]
+
[http://hackteria.org/wiki/index.php/The_June_9th_Image_Gallery The June 9th Image Gallery]
Images of the various results attained:
Images of the various results attained:

Revision as of 19:40, 21 October 2009

Week Four

June 8th- 17th

What are we trying to do?

We want the bacteria to lyse, to kill itself from the inside.

Well then, to do that we had to get a lysis gene in it. The thing is, with the lysis gene needs to be turned on by this specific substance called a promoter

There are two kinds of promoters, ones that make the gene they're attached to , be 'on' all the time. Then, you have ones that turn on when you want them to, in our case, when you add a mystery enzyme.

Our Step-wise Process:

How we tried to do this:

Step 1 - Taking dry DNA from wells

Step 2 - Transforming competent cells

Step 3 - Picking a single colony.

Step 4 - Inoculating broth with Ampicillin-R (an antibiotic) and letting it grow for 18 hours.

Step 5 - Using the resulting culture for mini-prep. - a process used to purify plasmids and yields clean, usable DNA.

Step 6 - Digesting the DNA

Step 7 - Gel Electrophoresis

Step 8- Ligation

Step 9- Transformation and Inoculation

June 9th

The June 9th Image Gallery

Images of the various results attained: