Team:ArtScienceBangalore/Parts
From 2009.igem.org
(Difference between revisions)
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- | <strong>Here is the part that we submitted to the Standard Parts Registry: <br> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K221000">BBa_K221000</a></strong><br> | + | <strong>Here is the part that we submitted to the Standard Parts Registry: <br> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K221000"><>strong>BBa_K221000</strong></a></strong><br> |
<strong><i>This helps our construct synthesize geosmin, the enzyme that smells like rain.</i></strong><br> | <strong><i>This helps our construct synthesize geosmin, the enzyme that smells like rain.</i></strong><br> | ||
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<strong> <b>Amino acid sequence:</b> </strong><br> | <strong> <b>Amino acid sequence:</b> </strong><br> | ||
- | + | MTQQPFQLPHFYLPHPARLNPHLDEARAHSTTWAREMGMLEGSGVWEQSDLEAHDYGLLCAYTHPDCDGPALSLITDWYVWVFFFDDHFLEK<br> | |
- | + | YKRSQDRLAGKAHLDRLPLFMPLDDAAGMPEPRNPVEAGLADLWTRTVPAMSADWRRRFAVATEHLLNESMWELSNINEGRVANPVEYIEM<br> | |
- | + | RRKVGGAPWSAGLVEYATAEVPAAVAGTRPLRVLMETFSDAVHLRNDLFSYQREVEDEGELSNGVLVLETFFGCTTQEAADLVNDVLTSRL<br> | |
- | + | HQFEHTAFTEVPAVALEKGLTPLEVAAVGAYTKGLQDWQSGGHEWHMRSSRYMNKGERPLAGWQALTGPGTSAADVGALLADAVAQRARSYT | |
</div> | </div> |
Revision as of 15:08, 21 October 2009
Parts
Here is the part that we submitted to the Standard Parts Registry:
<>strong>BBa_K221000
This helps our construct synthesize geosmin, the enzyme that smells like rain.
This part codes N-terminal (366 amino acid) domain of the SCO6073 gene from S. coelicolor A3.
Expression of this domain gives a fully functional germacradienol synthase with steady-state
catalytic parameters similar to those of the full-length protein that converts farnesyl diphosphate(FPP) to Geosmin*.
Amino acid sequence:
MTQQPFQLPHFYLPHPARLNPHLDEARAHSTTWAREMGMLEGSGVWEQSDLEAHDYGLLCAYTHPDCDGPALSLITDWYVWVFFFDDHFLEK
YKRSQDRLAGKAHLDRLPLFMPLDDAAGMPEPRNPVEAGLADLWTRTVPAMSADWRRRFAVATEHLLNESMWELSNINEGRVANPVEYIEM
RRKVGGAPWSAGLVEYATAEVPAAVAGTRPLRVLMETFSDAVHLRNDLFSYQREVEDEGELSNGVLVLETFFGCTTQEAADLVNDVLTSRL
HQFEHTAFTEVPAVALEKGLTPLEVAAVGAYTKGLQDWQSGGHEWHMRSSRYMNKGERPLAGWQALTGPGTSAADVGALLADAVAQRARSYT
<>strong>BBa_K221000
This helps our construct synthesize geosmin, the enzyme that smells like rain.
This part codes N-terminal (366 amino acid) domain of the SCO6073 gene from S. coelicolor A3.
Expression of this domain gives a fully functional germacradienol synthase with steady-state
catalytic parameters similar to those of the full-length protein that converts farnesyl diphosphate(FPP) to Geosmin*.
Amino acid sequence:
MTQQPFQLPHFYLPHPARLNPHLDEARAHSTTWAREMGMLEGSGVWEQSDLEAHDYGLLCAYTHPDCDGPALSLITDWYVWVFFFDDHFLEK
YKRSQDRLAGKAHLDRLPLFMPLDDAAGMPEPRNPVEAGLADLWTRTVPAMSADWRRRFAVATEHLLNESMWELSNINEGRVANPVEYIEM
RRKVGGAPWSAGLVEYATAEVPAAVAGTRPLRVLMETFSDAVHLRNDLFSYQREVEDEGELSNGVLVLETFFGCTTQEAADLVNDVLTSRL
HQFEHTAFTEVPAVALEKGLTPLEVAAVGAYTKGLQDWQSGGHEWHMRSSRYMNKGERPLAGWQALTGPGTSAADVGALLADAVAQRARSYT