http://2009.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden&feed=atom&action=historyTeam:BIOTEC Dresden - Revision history2024-03-28T23:42:33ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden&diff=161954&oldid=prevDivyail at 00:39, 22 October 20092009-10-22T00:39:36Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===<center>Factory-on-a-chip: Temporal and spatial control of protein synthesis </center>===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===<center>Factory-on-a-chip: Temporal and spatial control of protein synthesis </center>===</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Organizing matter on micro- and nano-scale requires specialized tools that control how components are assembled, when and where they are added, and how the product is transported. Our factory-on-a-chip is designed to do just that. In this project we conduct a two-step assembly process of biological and metallic components in microvesicles of picolitre reaction volumes. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Organizing matter on micro- and nano-scale requires specialized tools that control how components are assembled, when and where they are added, and how the product is transported. Our factory-on-a-chip is designed to do just that. In this project we conduct a two-step assembly process of biological and metallic components in microvesicles of picolitre reaction volumes. </div></td></tr>
</table>Divyailhttp://2009.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden&diff=161928&oldid=prevDivyail at 00:38, 22 October 20092009-10-22T00:38:19Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>===<center>Factory-on-a-chip: Temporal and spatial control of protein synthesis by ''in vitro'' recombination inside picoliter reactors</center>===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>===<center>Factory-on-a-chip: Temporal and spatial control of protein synthesis <ins class="diffchange diffchange-inline"></center>===</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">===<center></ins>by ''in vitro'' recombination inside picoliter reactors</center>===</div></td></tr>
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</table>Divyailhttp://2009.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden&diff=161886&oldid=prevDivyail at 00:36, 22 October 20092009-10-22T00:36:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===<center>Factory-on-a-chip: Temporal and spatial control of protein synthesis by ''in vitro'' recombination inside picoliter reactors</center>===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===<center>Factory-on-a-chip: Temporal and spatial control of protein synthesis by ''in vitro'' recombination inside picoliter reactors</center>===</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Organizing matter on micro- and nano-scale requires specialized tools that control how components are assembled, when and where they are added, and how the product is transported. Our factory-on-a-chip is designed to do just that. In this project we conduct a two-step assembly process of biological and metallic components in microvesicles of picolitre reaction volumes. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Organizing matter on micro- and nano-scale requires specialized tools that control how components are assembled, when and where they are added, and how the product is transported. Our factory-on-a-chip is designed to do just that. In this project we conduct a two-step assembly process of biological and metallic components in microvesicles of picolitre reaction volumes. </div></td></tr>
</table>Divyailhttp://2009.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden&diff=161206&oldid=prevDherde at 00:15, 22 October 20092009-10-22T00:15:30Z<p></p>
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</table>Dherdehttp://2009.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden&diff=159942&oldid=prevDherde at 23:38, 21 October 20092009-10-21T23:38:07Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Production and packaging in picolitre reactors allows for precise control of all components of the product. The reactors are vesicles moving through a microfluidic chamber. The transparency of the reactors and flow chamber enables us to monitor the whole production process. Enclosed in the vesicles are a synthetic in-vitro transcription-translation kit, a plasmid coding for a genetic timer and a modified protein, as well as raw materials for silver nanoparticles. The genetic timer determines the onset of transcription and marks the beginning of the production process. It is a genetic circuit based on Flp recombinase. The timer is set off depending on the distance between two recombination sites. A genetic timer allows to add components sequentially or at a specific time. The protein we express is a GFP tagged with a sequence which is known to nucleate silver nanoparticle formation. An engineer should be able to use any raw material for his product to have the desired properties. Yet traditionally, there is a pronounced distinction between classical and biological engineering. We try to overcome this schism by integrating protein expression and sequence-specific metallization of our modified protein with silver in one manufacturing process. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Production and packaging in picolitre reactors allows for precise control of all components of the product. The reactors are vesicles moving through a microfluidic chamber. The transparency of the reactors and flow chamber enables us to monitor the whole production process. Enclosed in the vesicles are a synthetic in-vitro transcription-translation kit, a plasmid coding for a genetic timer and a modified protein, as well as raw materials for silver nanoparticles. </div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The genetic timer determines the onset of transcription and marks the beginning of the production process. It is a genetic circuit based on Flp recombinase. The timer is set off depending on the distance between two recombination sites. A genetic timer allows to add components sequentially or at a specific time. </div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The protein we express is a GFP tagged with a sequence which is known to nucleate silver nanoparticle formation. An engineer should be able to use any raw material for his product to have the desired properties. Yet traditionally, there is a pronounced distinction between classical and biological engineering. We try to overcome this schism by integrating protein expression and sequence-specific metallization of our modified protein with silver in one manufacturing process. </div></td></tr>
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</table>Dherdehttp://2009.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden&diff=157638&oldid=prevTomek at 22:31, 21 October 20092009-10-21T22:31:40Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===<center>Factory-on-a-chip: Temporal and spatial control of protein synthesis by ''in vitro'' recombination inside picoliter reactors</center>===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===<center>Factory-on-a-chip: Temporal and spatial control of protein synthesis by ''in vitro'' recombination inside picoliter reactors</center>===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Organizing matter on micro- and nano-scale requires specialized tools that control how components are assembled, when and where they are added, and how the product is transported. Our factory-on-a-chip is designed to do just that. In this project we conduct a two-step assembly process of biological and metallic components in microvesicles of picolitre reaction volumes<del class="diffchange diffchange-inline">. Production and packaging in picolitre reactors allows for precise control of all components of the product. The reactors are vesicles moving through a microfluidic chamber. The transparency of the reactors and flow chamber enables us to monitor the whole production process. Enclosed in the vesicles are a synthetic in-vitro transcription-translation kit, a plasmid coding for a genetic timer and a modified protein, as well as raw materials for silver nanoparticles. The genetic timer determines the onset of transcription and marks the beginning of the production process. It is a genetic circuit based on Flp recombinase. The timer is set off depending on the distance between two recombination sites. A genetic timer allows to add components sequentially or at a specific time. The protein we express is a GFP tagged with a sequence which is known to nucleate silver nanoparticle formation. An engineer should be able to use any raw material for his product to have the desired properties. Yet traditionally, there is a pronounced distinction between classical and biological engineering. We try to overcome this schism by integrating protein expression and sequence-specific metallization of our modified protein with silver in one manufacturing process</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Organizing matter on micro- and nano-scale requires specialized tools that control how components are assembled, when and where they are added, and how the product is transported. Our factory-on-a-chip is designed to do just that. In this project we conduct a two-step assembly process of biological and metallic components in microvesicles of picolitre reaction volumes. </div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Production and packaging in picolitre reactors allows for precise control of all components of the product. The reactors are vesicles moving through a microfluidic chamber. The transparency of the reactors and flow chamber enables us to monitor the whole production process. Enclosed in the vesicles are a synthetic in-vitro transcription-translation kit, a plasmid coding for a genetic timer and a modified protein, as well as raw materials for silver nanoparticles. The genetic timer determines the onset of transcription and marks the beginning of the production process. It is a genetic circuit based on Flp recombinase. The timer is set off depending on the distance between two recombination sites. A genetic timer allows to add components sequentially or at a specific time. The protein we express is a GFP tagged with a sequence which is known to nucleate silver nanoparticle formation. An engineer should be able to use any raw material for his product to have the desired properties. Yet traditionally, there is a pronounced distinction between classical and biological engineering. We try to overcome this schism by integrating protein expression and sequence-specific metallization of our modified protein with silver in one manufacturing process. </ins></div></td></tr>
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</table>Tomekhttp://2009.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden&diff=157610&oldid=prevTomek at 22:30, 21 October 20092009-10-21T22:30:31Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>===<center>Temporal and spatial control of protein synthesis by ''in vitro'' recombination inside picoliter reactors</center>===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>===<center><ins class="diffchange diffchange-inline">Factory-on-a-chip: </ins>Temporal and spatial control of protein synthesis by ''in vitro'' recombination inside picoliter reactors</center>===</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Organizing matter on micro- and nano-scale requires specialized tools that control how components are assembled, when and where they are added, and how the product is transported. Our factory-on-a-chip is designed to do just that. In this project we conduct a two-step assembly process of biological and metallic components in microvesicles of picolitre reaction volumes. Production and packaging in picolitre reactors allows for precise control of all components of the product. The reactors are vesicles moving through a microfluidic chamber. The transparency of the reactors and flow chamber enables us to monitor the whole production process. Enclosed in the vesicles are a synthetic in-vitro transcription-translation kit, a plasmid coding for a genetic timer and a modified protein, as well as raw materials for silver nanoparticles. The genetic timer determines the onset of transcription and marks the beginning of the production process. It is a genetic circuit based on Flp recombinase. The timer is set off depending on the distance between two recombination sites. A genetic timer allows to add components sequentially or at a specific time. The protein we express is a GFP tagged with a sequence which is known to nucleate silver nanoparticle formation. An engineer should be able to use any raw material for his product to have the desired properties. Yet traditionally, there is a pronounced distinction between classical and biological engineering. We try to overcome this schism by integrating protein expression and sequence-specific metallization of our modified protein with silver in one manufacturing process. </ins></div></td></tr>
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</table>Tomekhttp://2009.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden&diff=156845&oldid=prevSal9000 at 22:02, 21 October 20092009-10-21T22:02:29Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">===<center>Temporal and spatial control of protein synthesis by ''in vitro'' recombination inside picoliter reactors</center>===</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2009/4/4b/Anya_rabota_3.jpg" width="700" height="460" border="0" usemap="#map" /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2009/4/4b/Anya_rabota_3.jpg" width="700" height="460" border="0" usemap="#map" /></div></td></tr>
</table>Sal9000http://2009.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden&diff=153535&oldid=prevDherde at 20:10, 21 October 20092009-10-21T20:10:33Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:BIOTEC_Dresden/NewTemplateNoBar}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:BIOTEC_Dresden/NewTemplateNoBar}}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">=== Welcome to the Wiki of Team Biotec ===</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">We are the team from [http://www.biotec.tu-dresden.de/ Biotec Dresden], participating in iGEM for the first time this year. It's a real learning experience.</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Here is a video of a microfluidic chamber constructed for this project creating lipid vesicles:</del></div></td><td colspan="2"> </td></tr>
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</table>Dherdehttp://2009.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden&diff=153449&oldid=prevDherde at 20:07, 21 October 20092009-10-21T20:07:38Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Welcome to the Wiki of Team Biotec ===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Welcome to the Wiki of Team Biotec ===</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><img src="https://static.igem.org/mediawiki/2009/4/4b/Anya_rabota_3.jpg" width="700" height="460" border="0" usemap="#map" /></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><!-- #$VERSION:2.3 --></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><!-- #$AUTHOR:Daniel Herde --></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><area shape="poly" coords="394,332,325,335,289,309,289,206,367,203,391,221" alt="Timer" href="https://2009.igem.org/Team:BIOTEC_Dresden/Notebook_Recombinase" /></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><area shape="poly" coords="40,271,92,252,93,149,38,169" alt="Contact" href="https://2009.igem.org/Team:BIOTEC_Dresden/Contact_v2" /></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><area shape="poly" coords="595,202,592,122,527,124,529,207" alt="Lab Notebook" href="https://2009.igem.org/Team:BIOTEC_Dresden/Notebook_v2" /></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We are the team from [http://www.biotec.tu-dresden.de/ Biotec Dresden], participating in iGEM for the first time this year. It's a real learning experience.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We are the team from [http://www.biotec.tu-dresden.de/ Biotec Dresden], participating in iGEM for the first time this year. It's a real learning experience.</div></td></tr>
</table>Dherde