http://2009.igem.org/wiki/index.php?title=Team:Bay_Area_RSI&feed=atom&action=historyTeam:Bay Area RSI - Revision history2024-03-29T11:10:11ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:Bay_Area_RSI&diff=160495&oldid=prevIbezabeh at 23:54, 21 October 20092009-10-21T23:54:49Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|align="center"|<br><br>''Breast cancer is the second most common type of cancer diagnosed in women. RNAi has proven to be an effective mechanism in the silencing of oncogenes. Therefore, we have attempted to build a viable system for the delivery of RNAi into breast cancer cells. First, we inserted a shRNA sequence coding for the Raf-1 protein into an AAV cassette containing two ITR's, allowing it to reproduce itself in mammalian cells. This cassette was inserted into our chosen vector, the filamentous bacteriophage FUSE-55. An antibody sequence was then added to the phage plasmid near the coat protein sequence in order to target HER2. As an additional feature, we have fused Silicatein and Silintaphin to mStrepavidin, which will bind to a protein tag in the coat, forming silicate structures on the coat of the phage, thereby reducing the immunotoxicity of the bacteriophage in vivo. ''<br><br><br><br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|align="center"|<br><br>''<ins class="diffchange diffchange-inline"><font size="4"></ins>Breast cancer is the second most common type of cancer diagnosed in women. RNAi has proven to be an effective mechanism in the silencing of oncogenes. Therefore, we have attempted to build a viable system for the delivery of RNAi into breast cancer cells. First, we inserted a shRNA sequence coding for the Raf-1 protein into an AAV cassette containing two ITR's, allowing it to reproduce itself in mammalian cells. This cassette was inserted into our chosen vector, the filamentous bacteriophage FUSE-55. An antibody sequence was then added to the phage plasmid near the coat protein sequence in order to target HER2. As an additional feature, we have fused Silicatein and Silintaphin to mStrepavidin, which will bind to a protein tag in the coat, forming silicate structures on the coat of the phage, thereby reducing the immunotoxicity of the bacteriophage in vivo. <ins class="diffchange diffchange-inline"></font></ins>''<br><br><br><br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">[[Team:Bay_Area_RSI | Our Team]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
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</table>Ibezabehhttp://2009.igem.org/wiki/index.php?title=Team:Bay_Area_RSI&diff=160404&oldid=prevIbezabeh at 23:51, 21 October 20092009-10-21T23:51:34Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''Breast cancer is the second most common type of cancer diagnosed in women. RNAi has proven to be an effective mechanism in the silencing of oncogenes. Therefore, we have attempted to build a viable system for the delivery of RNAi into breast cancer cells. First, we inserted a shRNA sequence coding for the Raf-1 protein into an AAV cassette containing two ITR's, allowing it to reproduce itself in mammalian cells. This cassette was inserted into our chosen vector, the filamentous bacteriophage FUSE-55. An antibody sequence was then added to the phage plasmid near the coat protein sequence in order to target HER2. As an additional feature, we have fused Silicatein and Silintaphin to mStrepavidin, which will bind to a protein tag in the coat, forming silicate structures on the coat of the phage, thereby reducing the immunotoxicity of the bacteriophage in vivo. ''</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|align="center"|[[Team:Bay_Area_RSI | Our Team]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|align="center"|<ins class="diffchange diffchange-inline"><br><br>''Breast cancer is the second most common type of cancer diagnosed in women. RNAi has proven to be an effective mechanism in the silencing of oncogenes. Therefore, we have attempted to build a viable system for the delivery of RNAi into breast cancer cells. First, we inserted a shRNA sequence coding for the Raf-1 protein into an AAV cassette containing two ITR's, allowing it to reproduce itself in mammalian cells. This cassette was inserted into our chosen vector, the filamentous bacteriophage FUSE-55. An antibody sequence was then added to the phage plasmid near the coat protein sequence in order to target HER2. As an additional feature, we have fused Silicatein and Silintaphin to mStrepavidin, which will bind to a protein tag in the coat, forming silicate structures on the coat of the phage, thereby reducing the immunotoxicity of the bacteriophage in vivo. ''<br><br><br><br><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Team:Bay_Area_RSI | Our Team]]</div></td></tr>
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</table>Ibezabehhttp://2009.igem.org/wiki/index.php?title=Team:Bay_Area_RSI&diff=160169&oldid=prevIbezabeh at 23:44, 21 October 20092009-10-21T23:44:44Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
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</table>Ibezabehhttp://2009.igem.org/wiki/index.php?title=Team:Bay_Area_RSI&diff=123280&oldid=prevTsrivastava at 00:55, 20 October 20092009-10-20T00:55:39Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Breast cancer is the second most common type of cancer diagnosed in women. RNAi has proven to be an effective mechanism in the silencing of oncogenes. Therefore, we have attempted to build a viable system for the delivery of RNAi into breast cancer cells. First, we inserted a shRNA sequence coding for the Raf-1 protein into an AAV cassette containing two ITR's, allowing it to reproduce itself in mammalian cells. This cassette was inserted into our chosen vector, the filamentous bacteriophage FUSE-55. An antibody sequence was then added to the phage plasmid near the coat protein sequence in order to target HER2. As an additional feature, we have fused Silicatein and Silintaphin to mStrepavidin, which will bind to a protein tag in the coat, forming silicate structures on the coat of the phage, thereby reducing the immunotoxicity of the bacteriophage in vivo. ''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Breast cancer is the second most common type of cancer diagnosed in women. RNAi has proven to be an effective mechanism in the silencing of oncogenes. Therefore, we have attempted to build a viable system for the delivery of RNAi into breast cancer cells. First, we inserted a shRNA sequence coding for the Raf-1 protein into an AAV cassette containing two ITR's, allowing it to reproduce itself in mammalian cells. This cassette was inserted into our chosen vector, the filamentous bacteriophage FUSE-55. An antibody sequence was then added to the phage plasmid near the coat protein sequence in order to target HER2. As an additional feature, we have fused Silicatein and Silintaphin to mStrepavidin, which will bind to a protein tag in the coat, forming silicate structures on the coat of the phage, thereby reducing the immunotoxicity of the bacteriophage in vivo. ''</div></td></tr>
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</table>Tsrivastavahttp://2009.igem.org/wiki/index.php?title=Team:Bay_Area_RSI&diff=122913&oldid=prevTsrivastava at 23:56, 19 October 20092009-10-19T23:56:50Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>''<del class="diffchange diffchange-inline">Tell us more about your project</del>. <del class="diffchange diffchange-inline"> Give us background</del>. <del class="diffchange diffchange-inline"> Use this is </del>the <del class="diffchange diffchange-inline">abstract </del>of <del class="diffchange diffchange-inline">your project</del>. <del class="diffchange diffchange-inline"> Be descriptive but concise (</del>1-<del class="diffchange diffchange-inline">2 paragraphs)</del>''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>''<ins class="diffchange diffchange-inline">Breast cancer is the second most common type of cancer diagnosed in women</ins>. <ins class="diffchange diffchange-inline">RNAi has proven to be an effective mechanism in the silencing of oncogenes</ins>. <ins class="diffchange diffchange-inline">Therefore, we have attempted to build a viable system for </ins>the <ins class="diffchange diffchange-inline">delivery </ins>of <ins class="diffchange diffchange-inline">RNAi into breast cancer cells</ins>. <ins class="diffchange diffchange-inline">First, we inserted a shRNA sequence coding for the Raf-</ins>1 <ins class="diffchange diffchange-inline">protein into an AAV cassette containing two ITR's, allowing it to reproduce itself in mammalian cells. This cassette was inserted into our chosen vector, the filamentous bacteriophage FUSE</ins>-<ins class="diffchange diffchange-inline">55. An antibody sequence was then added to the phage plasmid near the coat protein sequence in order to target HER2. As an additional feature, we have fused Silicatein and Silintaphin to mStrepavidin, which will bind to a protein tag in the coat, forming silicate structures on the coat of the phage, thereby reducing the immunotoxicity of the bacteriophage in vivo. </ins>''</div></td></tr>
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</table>Tsrivastavahttp://2009.igem.org/wiki/index.php?title=Team:Bay_Area_RSI&diff=122853&oldid=prevTsrivastava at 23:46, 19 October 20092009-10-19T23:46:28Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.</div></td></tr>
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</table>Tsrivastavahttp://2009.igem.org/wiki/index.php?title=Team:Bay_Area_RSI&diff=122841&oldid=prevTsrivastava at 23:45, 19 October 20092009-10-19T23:45:31Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|[[Image:<del class="diffchange diffchange-inline">RSI logo</del>|200px|right|frame]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|[[Image:|200px|right|frame]]</div></td></tr>
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</table>Tsrivastavahttp://2009.igem.org/wiki/index.php?title=Team:Bay_Area_RSI&diff=122808&oldid=prevTsrivastava at 23:40, 19 October 20092009-10-19T23:40:43Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|[[Image:<del class="diffchange diffchange-inline">Example_logo.png</del>|200px|right|frame]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|[[Image:<ins class="diffchange diffchange-inline">RSI logo</ins>|200px|right|frame]]</div></td></tr>
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</table>Tsrivastavahttp://2009.igem.org/wiki/index.php?title=Team:Bay_Area_RSI&diff=122806&oldid=prevTsrivastava at 23:40, 19 October 20092009-10-19T23:40:25Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|[[Image:<del class="diffchange diffchange-inline">RSI logo</del>|400px|thumb]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|[[Image:<ins class="diffchange diffchange-inline">IMG_1569.JPG</ins>|400px|thumb]]</div></td></tr>
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</table>Tsrivastavahttp://2009.igem.org/wiki/index.php?title=Team:Bay_Area_RSI&diff=122785&oldid=prevTsrivastava at 23:38, 19 October 20092009-10-19T23:38:20Z<p></p>
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</table>Tsrivastava