Team:Berkeley Wetlab

From 2009.igem.org

(Difference between revisions)
 
(250 intermediate revisions not shown)
Line 1: Line 1:
-
Preliminary project description
+
{{newtemplateBerkeley}}<br>
-
The University of California Berkeley iGem team is proposing to expand the design space of synthetic biology by exploring novel applications of cell surface display within Escherichia coli, the gold standard organism for bacterial engineering. The team envisions a bottom-up design scheme in order to tackle this engineering problem in a well organized, modular fashion. In order to overcome the challenges of engineering Escherichia coli cell surface display, a high throughput, automated, combinatorial strategy is employed to control the system.
+
<center> [[Image:Display-o-matic2.png|550px]] </center> <br>
 +
<big><font size="5" face="Book Antiqua"> Abstract</font> </big> <br>
 +
Cell surface display in ''E. coli'' tethers proteins to the outer membrane in order to localize them to the extracellular environment.  While this form of localization has allowed many novel functions to be engineered into ''E. coli'', work within this space relies on a trial and error approach rather than design principles. We worked to create a foundation of research which would make the rational design of cell surface display systems in ''E. coli''  possible.  We used a combinatorial approach to compare the ability of different display proteins to display different classes of functional proteins.  This required the development and implementation of an automated assembly method able to construct the large number of devices necessary to draw meaningful conclusions about design within this space.  
-
 
+
<br>
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
<br>
-
 
+
<br>
-
<html>
+
<center><font size="5" face="Book Antiqua">Our Team</font></center> <br>
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
[[Image:BerkeleyWetlabTeam.jpg|center|600px]]
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
<br>
-
This is a template page. READ THESE INSTRUCTIONS.
+
<br>
-
</div>
+
<font size="5" face="Book Antiqua">Acknowledgements</font> <br>
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
We thank our wonderful advisers: Chris Anderson, Terry Johnson, and Lane Weaver for their guidance and support. We also thank our generous sponsors:<br>
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
+
<br>
-
</div>
+
<br>
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
[[Image:berkeleyinvitrogen.jpg|200px]]
-
You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook. PLEASE keep all of your pages within your teams namespace. 
+
[[Image:berkeleynsf.jpg|100px]]
-
</div>
+
[[Image:berkeleyqbs.jpg|100px]]
-
</div>
+
[[Image:berkeleysynberg.png|350px]]<br>
-
</html>
+
<br>
-
 
+
-
<!-- *** End of the alert box *** -->
+
-
 
+
-
 
+
-
 
+
-
{|align="justify"
+
-
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
+
-
|[[Image:Example_logo.png|200px|right|frame]]
+
-
|-
+
-
|
+
-
''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
+
-
|[[Image:Team.png|right|frame|Your team picture]]
+
-
|-
+
-
|
+
-
|align="center"|[[Team:Berkeley_Wetlab | Team Example]]
+
-
|}
+
-
 
+
-
<!--- The Mission, Experiments --->
+
-
 
+
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
-
!align="center"|[[Team:Berkeley_Wetlab|Home]]
+
-
!align="center"|[[Team:Berkeley_Wetlab/Team|The Team]]
+
-
!align="center"|[[Team:Berkeley_Wetlab/Project|The Project]]
+
-
!align="center"|[[Team:Berkeley_Wetlab/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:Berkeley_Wetlab/Modeling|Modeling]]
+
-
!align="center"|[[Team:Berkeley_Wetlab/Notebook|Notebook]]
+
-
|}
+
-
(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
+

Latest revision as of 19:14, 21 October 2009


Display-o-matic2.png

Abstract
Cell surface display in E. coli tethers proteins to the outer membrane in order to localize them to the extracellular environment. While this form of localization has allowed many novel functions to be engineered into E. coli, work within this space relies on a trial and error approach rather than design principles. We worked to create a foundation of research which would make the rational design of cell surface display systems in E. coli possible. We used a combinatorial approach to compare the ability of different display proteins to display different classes of functional proteins. This required the development and implementation of an automated assembly method able to construct the large number of devices necessary to draw meaningful conclusions about design within this space.




Our Team

BerkeleyWetlabTeam.jpg



Acknowledgements
We thank our wonderful advisers: Chris Anderson, Terry Johnson, and Lane Weaver for their guidance and support. We also thank our generous sponsors:


Berkeleyinvitrogen.jpg Berkeleynsf.jpg Berkeleyqbs.jpg Berkeleysynberg.png