http://2009.igem.org/wiki/index.php?title=Team:Berkeley_Wetlab/Automation&feed=atom&action=historyTeam:Berkeley Wetlab/Automation - Revision history2024-03-28T23:07:19ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:Berkeley_Wetlab/Automation&diff=161181&oldid=prevJennbrophy: /* oligoDesigner */2009-10-22T00:14:56Z<p><span class="autocomment">oligoDesigner</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''IDT Ordering Form'''<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''IDT Ordering Form'''<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>||[[Image:Berkeleyarrow.png|25px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>||[[Image:Berkeleyarrow.png|25px]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>||[[Image:IDT96berkeley.png|<del class="diffchange diffchange-inline">100px</del>]]<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>||[[Image:IDT96berkeley.png|<ins class="diffchange diffchange-inline">125px</ins>]]<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center>'''IDT 96 well plate'''</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center>'''IDT 96 well plate'''</center></div></td></tr>
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</table>Jennbrophyhttp://2009.igem.org/wiki/index.php?title=Team:Berkeley_Wetlab/Automation&diff=161174&oldid=prevJennbrophy: /* oligoDesigner */2009-10-22T00:14:42Z<p><span class="autocomment">oligoDesigner</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''IDT Ordering Form'''<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''IDT Ordering Form'''<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>||[[Image:Berkeleyarrow.png|25px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>||[[Image:Berkeleyarrow.png|25px]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>||[[Image:IDT96berkeley.png|<del class="diffchange diffchange-inline">30px</del>]]<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>||[[Image:IDT96berkeley.png|<ins class="diffchange diffchange-inline">100px</ins>]]<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center>'''IDT 96 well plate'''</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center>'''IDT 96 well plate'''</center></div></td></tr>
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</table>Jennbrophyhttp://2009.igem.org/wiki/index.php?title=Team:Berkeley_Wetlab/Automation&diff=161150&oldid=prevJennbrophy: /* oligoDesigner */2009-10-22T00:14:12Z<p><span class="autocomment">oligoDesigner</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''IDT Ordering Form'''<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''IDT Ordering Form'''<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>||[[Image:Berkeleyarrow.png|25px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>||[[Image:Berkeleyarrow.png|25px]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>||<del class="diffchange diffchange-inline">(</del>IDT 96 well plate <del class="diffchange diffchange-inline">image)</del><<del class="diffchange diffchange-inline">br</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>||<ins class="diffchange diffchange-inline">[[Image:IDT96berkeley.png|30px]]<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><center>'''</ins>IDT 96 well plate<ins class="diffchange diffchange-inline">'''</ins><<ins class="diffchange diffchange-inline">/center</ins>></div></td></tr>
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</table>Jennbrophyhttp://2009.igem.org/wiki/index.php?title=Team:Berkeley_Wetlab/Automation&diff=160524&oldid=prevJennbrophy: /* Success! */2009-10-21T23:55:42Z<p><span class="autocomment">Success!</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:BerkeleyAssemblyAutomation.png|800px|center]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:BerkeleyAssemblyAutomation.png|800px|center]]</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==Success<del class="diffchange diffchange-inline">!</del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==Success==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Using our newly developed automated assembly processes we were able to make over 800 parts (both basic and composite) in one summer! This magnitude of part making is unprecedented in iGEM competitions and enabled us to test a very large number of variations on cell surface display systems. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Using our newly developed automated assembly processes we were able to make over 800 parts (both basic and composite) in one summer! This magnitude of part making is unprecedented in iGEM competitions and enabled us to test a very large number of variations on cell surface display systems. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Success Rates===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Success Rates===</div></td></tr>
</table>Jennbrophyhttp://2009.igem.org/wiki/index.php?title=Team:Berkeley_Wetlab/Automation&diff=160509&oldid=prevJennbrophy: /* Success Rates */2009-10-21T23:55:07Z<p><span class="autocomment">Success Rates</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Stats:'''<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Stats:'''<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* <7% of every assembly set yielded no colonies</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* <7% of every assembly set yielded no colonies</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* 12-15% of the colonies in each set that did grow were co-transformed (meaning the cells took up more than one of our specialized assembly vectors, which is undesirable)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* 12-15% of the colonies in each set that did grow were co-transformed (meaning the cells took up more than one of our specialized assembly vectors, which is undesirable)<ins class="diffchange diffchange-inline">. </ins>We have a simple screening test to check for co-transformation, so this was not a problem.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> -</del>We have a simple screening test to check for co-transformation, so this was not a problem.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* We sequenced approximately 10% of all final products, and found that 90% of the things we sequenced were correct. When assembly sets were found to be incorrect, we started the set over again from the last successful intermediate, and we were usually able to trace the flaw in the assembly to a human error. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* We sequenced approximately 10% of all final products, and found that 90% of the things we sequenced were correct. When assembly sets were found to be incorrect, we started the set over again from the last successful intermediate, and we were usually able to trace the flaw in the assembly to a human error. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The assembly scheme we developed works just as well as assembly by hand and is much faster. Visit Jenn Brophy and Gabriela Guzman's [https://2009.igem.org/Team:Berkeley_Wetlab/NoteBooks notebooks] for notes on assembly.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The assembly scheme we developed works just as well as assembly by hand and is much faster. Visit Jenn Brophy and Gabriela Guzman's [https://2009.igem.org/Team:Berkeley_Wetlab/NoteBooks notebooks] for notes on assembly.</div></td></tr>
</table>Jennbrophyhttp://2009.igem.org/wiki/index.php?title=Team:Berkeley_Wetlab/Automation&diff=160491&oldid=prevJennbrophy: /* Success Rates */2009-10-21T23:54:45Z<p><span class="autocomment">Success Rates</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* <7% of every assembly set yielded no colonies</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* <7% of every assembly set yielded no colonies</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 12-15% of the colonies in each set that did grow were co-transformed (meaning the cells took up more than one of our specialized assembly vectors, which is undesirable)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 12-15% of the colonies in each set that did grow were co-transformed (meaning the cells took up more than one of our specialized assembly vectors, which is undesirable)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> * </del>We have a simple screening test to check for co-transformation, so this was not a problem.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> -</ins>We have a simple screening test to check for co-transformation, so this was not a problem.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* We sequenced approximately 10% of all final products, and found that 90% of the things we sequenced were correct. When assembly sets were found to be incorrect, we started the set over again from the last successful intermediate, and we were usually able to trace the flaw in the assembly to a human error. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* We sequenced approximately 10% of all final products, and found that 90% of the things we sequenced were correct. When assembly sets were found to be incorrect, we started the set over again from the last successful intermediate, and we were usually able to trace the flaw in the assembly to a human error. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The assembly scheme we developed works just as well as assembly by hand and is much faster. Visit Jenn Brophy and Gabriela Guzman's [https://2009.igem.org/Team:Berkeley_Wetlab/NoteBooks notebooks] for notes on assembly.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The assembly scheme we developed works just as well as assembly by hand and is much faster. Visit Jenn Brophy and Gabriela Guzman's [https://2009.igem.org/Team:Berkeley_Wetlab/NoteBooks notebooks] for notes on assembly.</div></td></tr>
</table>Jennbrophyhttp://2009.igem.org/wiki/index.php?title=Team:Berkeley_Wetlab/Automation&diff=160478&oldid=prevJennbrophy: /* Success! */2009-10-21T23:54:20Z<p><span class="autocomment">Success!</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Using our newly developed automated assembly processes we were able to make over 800 parts (both basic and composite) in one summer! This magnitude of part making is unprecedented in iGEM competitions and enabled us to test a very large number of variations on cell surface display systems. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Using our newly developed automated assembly processes we were able to make over 800 parts (both basic and composite) in one summer! This magnitude of part making is unprecedented in iGEM competitions and enabled us to test a very large number of variations on cell surface display systems. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Success Rates===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Success Rates===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The assembly scheme we developed works just as well as assembly by hand and is much faster. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">'''Stats:'''<br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">* percentages</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* <7% of every assembly set yielded no colonies</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">* see </del>Jenn Brophy and Gabriela Guzman's [https://2009.igem.org/Team:Berkeley_Wetlab/NoteBooks notebooks] for notes on assembly.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* 12-15% of the colonies in each set that did grow were co-transformed (meaning the cells took up more than one of our specialized assembly vectors, which is undesirable)</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">* 2ab assembly</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> * We have a simple screening test to check for co-transformation, so this was not a problem.</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* We sequenced approximately 10% of all final products, and found that 90% of the things we sequenced were correct. When assembly sets were found to be incorrect, we started the set over again from the last successful intermediate, and we were usually able to trace the flaw in the assembly to a human error. </ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">We made a lot of parts with a robot.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The assembly scheme we developed works just as well as assembly by hand and is much faster. <ins class="diffchange diffchange-inline">Visit </ins>Jenn Brophy and Gabriela Guzman's [https://2009.igem.org/Team:Berkeley_Wetlab/NoteBooks notebooks] for notes on assembly.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><center>'''2008 Berkeley Wetlab Team'''</center></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><a href="https://2008.igem.org/Team:UC_Berkeley"></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <img width="200" src="https://static.igem.org/mediawiki/2009/c/c6/Clonebotsscreenshot.png"></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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</table>Jennbrophyhttp://2009.igem.org/wiki/index.php?title=Team:Berkeley_Wetlab/Automation&diff=160214&oldid=prevJennbrophy: /* Clotho and Liquid Handling Robot */2009-10-21T23:45:47Z<p><span class="autocomment">Clotho and Liquid Handling Robot</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To build composite parts we use another Clotho tool built by the 2009 Berkeley Computational Team. This Clotho tool, named Kepler, generates a series of commands for a liquid handling robot to build goal composite parts based on the assembly tree it generated. Kepler will also output a series of human readable instructions that can guide researchers though the un-automated steps of the assembly protocol. By utilizing a combination of specialized software, liquid handling hardware, and newly developed chemistry, we were able to automate our composite part assembly process.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To build composite parts we use another Clotho tool built by the 2009 Berkeley Computational Team. This Clotho tool, named Kepler, generates a series of commands for a liquid handling robot to build goal composite parts based on the assembly tree it generated. Kepler will also output a series of human readable instructions that can guide researchers though the un-automated steps of the assembly protocol. By utilizing a combination of specialized software, liquid handling hardware, and newly developed chemistry, we were able to automate our composite part assembly process.<br></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:BerkeleyAssemblyAutomation.png|<del class="diffchange diffchange-inline">600px</del>|center]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:BerkeleyAssemblyAutomation.png|<ins class="diffchange diffchange-inline">800px</ins>|center]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Success!==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Success!==</div></td></tr>
</table>Jennbrophyhttp://2009.igem.org/wiki/index.php?title=Team:Berkeley_Wetlab/Automation&diff=160191&oldid=prevJennbrophy: /* Clotho and Liquid Handling Robot */2009-10-21T23:45:16Z<p><span class="autocomment">Clotho and Liquid Handling Robot</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 23:45, 21 October 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To build composite parts we use another Clotho tool built by the 2009 Berkeley Computational Team. This Clotho tool, named Kepler, generates a series of commands for a liquid handling robot to build goal composite parts based on the assembly tree it generated. Kepler will also output a series of human readable instructions that can guide researchers though the un-automated steps of the assembly protocol. By utilizing a combination of specialized software, liquid handling hardware, and newly developed chemistry, we were able to automate our composite part assembly process.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To build composite parts we use another Clotho tool built by the 2009 Berkeley Computational Team. This Clotho tool, named Kepler, generates a series of commands for a liquid handling robot to build goal composite parts based on the assembly tree it generated. Kepler will also output a series of human readable instructions that can guide researchers though the un-automated steps of the assembly protocol. By utilizing a combination of specialized software, liquid handling hardware, and newly developed chemistry, we were able to automate our composite part assembly process.<br></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><</del>center<del class="diffchange diffchange-inline">>clotho(kepler)---->csv---->robot---> 96 well plate</center></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Success!==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Success!==</div></td></tr>
</table>Jennbrophyhttp://2009.igem.org/wiki/index.php?title=Team:Berkeley_Wetlab/Automation&diff=154911&oldid=prevJennbrophy: /* Clotho and Liquid Handling Robot */2009-10-21T20:56:19Z<p><span class="autocomment">Clotho and Liquid Handling Robot</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2AB assembly utilizes six different types of specialized assembly vectors to make composite parts. In order to make composite parts in the least number of steps, an assembly tree, outlining the assembly process, must be generated. Assembly trees specify which specialized assembly vector to put basic parts in and specify the order in which composite part intermediates should be made. Generating assembly trees manually can be very difficult, especially when you need to make a large number of complicated parts. This year Berkeley's Computational Team built a plugin for Clotho (their software project) to help us make assembly trees. After generating assembly trees with Clotho and gateway-ing our parts into the correct vectors we can begin to build composite parts. To read more about Clotho please visit the [https://2009.igem.org/Team:Berkeley_Software 2009 Berkeley Computational Team wiki].<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2AB assembly utilizes six different types of specialized assembly vectors to make composite parts. In order to make composite parts in the least number of steps, an assembly tree, outlining the assembly process, must be generated. Assembly trees specify which specialized assembly vector to put basic parts in and specify the order in which composite part intermediates should be made. Generating assembly trees manually can be very difficult, especially when you need to make a large number of complicated parts. This year Berkeley's Computational Team built a plugin for Clotho (their software project) to help us make assembly trees. After generating assembly trees with Clotho and gateway-ing our parts into the correct vectors we can begin to build composite parts. To read more about Clotho please visit the [https://2009.igem.org/Team:Berkeley_Software 2009 Berkeley Computational Team wiki].<br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To build composite parts we use another Clotho tool built by the 2009 Berkeley Computational Team. This Clotho tool, named Kepler, generates a series of commands for a liquid handling robot to build goal composite parts based on the assembly tree it generated. Kepler will also output a series of human readable instructions that can guide researchers though the un-automated steps of the assembly protocol. By utilizing a combination of specialized software, liquid handling hardware, and newly developed chemistry, we were able to automate our composite part assembly process.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To build composite parts we use another Clotho tool built by the 2009 Berkeley Computational Team. This Clotho tool, named Kepler, generates a series of commands for a liquid handling robot to build goal composite parts based on the assembly tree it generated. Kepler will also output a series of human readable instructions that can guide researchers though the un-automated steps of the assembly protocol. By utilizing a combination of specialized software, liquid handling hardware, and newly developed chemistry, we were able to automate our composite part assembly process.<br></div></td></tr>
</table>Jennbrophy