Team:Berkeley Wetlab/Passenger: Leucine Zippers

From 2009.igem.org

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==Functional Assay: Cell-Cell Clumping==
==Functional Assay: Cell-Cell Clumping==
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[[Image:IGem2009_leucine_zipper_cartoon.jpg |500px]]
<left> [[Image:iGem2009Berkeley_Cloudy.jpg |100px]]</left>
<left> [[Image:iGem2009Berkeley_Cloudy.jpg |100px]]</left>
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<left> [[Image:iGem2009Berkeley_Clear.jpg |100px]]</left>

Revision as of 04:29, 21 October 2009

Contents

Leucine Zippers

One potential use of cell surface display is to adhere cells together selectively such that there is structure. However, such a feat is impossible to do in one step. In order to work up to such a goal, the first step of such an endeavor is create a basic system of adhere 2 cells types together. We propse using dimeric leucine zippers to adhere cells to one another. Leucine zippers are short peptides which form alpha helices that compliment each other. Using a GNC4 leucine zipper heterodimers KILR and EILD, we hoped to adhere cells to one another.

Functional Assay: Cell-Cell Clumping

IGem2009 leucine zipper cartoon.jpg <left> IGem2009Berkeley Cloudy.jpg</left> <left> IGem2009Berkeley Clear.jpg</left>

Results

Data

Conclusions