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Team:Berkeley Wetlab/Passenger: Leucine Zippers
From 2009.igem.org
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==Functional Assay: Cell-Cell Adhesion== | ==Functional Assay: Cell-Cell Adhesion== | ||
'''This assay tests for the functionality of displayed leucine zippers via the leucine zipper's ability to dimerized and, as a result, pull the cells out of solution into a clump.'''<br> | '''This assay tests for the functionality of displayed leucine zippers via the leucine zipper's ability to dimerized and, as a result, pull the cells out of solution into a clump.'''<br> | ||
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[[Image:IGem2009_leucine_zipper_cartoon.jpg |500px]] | [[Image:IGem2009_leucine_zipper_cartoon.jpg |500px]] | ||
| - | + | [[Image:iGem2009Berkeley_Cloudy.jpg |100px]] | |
| - | + | [[Image:iGem2009Berkeley_Clear.jpg |100px]] | |
==Results== | ==Results== | ||
===Data=== | ===Data=== | ||
===Conclusions=== | ===Conclusions=== | ||
Revision as of 04:33, 21 October 2009
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Contents |
Leucine Zippers
One potential use of cell surface display is to adhere cells together selectively such that there is structure. However, such a feat is impossible to do in one step. In order to work up to such a goal, the first step of such an endeavor is create a basic system of adhere 2 cells types together. We propse using dimeric leucine zippers to adhere cells to one another. Leucine zippers are short peptides which form alpha helices that compliment each other. Using a GNC4 leucine zipper heterodimers KILR and EILD, we hoped to adhere cells to one another.
Functional Assay: Cell-Cell Adhesion
This assay tests for the functionality of displayed leucine zippers via the leucine zipper's ability to dimerized and, as a result, pull the cells out of solution into a clump.
