Team:Berkeley Wetlab/Passenger: TypeIII Needle scFv

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dot blot and visualization

  1. pipet 5ul of cell lysate onto nitrocellulose paper and allow to dry completely
  2. wet nitrocellulose with 10ml of 1X TBST for 5minutes
  3. block with 2% BSA (.2g BSA in 10ml 1X TBST)for 1 hour
  4. wash 3X with TBST - 5minutes/wash
  5. add 4ml of 1:500 Anti-His HRP: TBST, wait 30minutes
  6. wash 3X with TBST - 5minutes/wash
  7. leave the last wash on for developing
  8. add 3mls of chemiluminescent reagents to the nitrocellulose paper and take pictures
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