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Team:Bologna
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= Project Summary = | = Project Summary = | ||
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More details about our work in the [[Team:Bologna/Project|Project]] section. | More details about our work in the [[Team:Bologna/Project|Project]] section. | ||
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= Acknowledgements = | = Acknowledgements = | ||
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* ''' [http://www.unibo.it/Portale/default.htm University of Bologna] ''' | * ''' [http://www.unibo.it/Portale/default.htm University of Bologna] ''' | ||
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Revision as of 10:45, 16 October 2009
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Project Summary
Which is our idea?
The project aims to implement a protein synthesis regulation system in Escherichia coli that acts at translational level regardless of the target gene to be downregulated. This "general-purpose" device could allow a faster control of protein expression. Our device was named T-Rex (Trans Repressor of Expression). It consists of two new BioBricks, i.e. the Trans-repressor and the Cis-repressing.
How can we achieve our goal?
The TRANS-repressor is a non-coding DNA sequence that acts as a silencer of the CIS-repressing mRNA. In fact, the Cis-repressing sequence includes a TRANS-repressor complementary region ending with a ribosome binding site (RBS). Moreover, it is assembled upstream of the target gene coding sequence. When the TRANS-repressor and the CIS-repressing mRNAs bind together, the RBS recognition by the ribosome is prevented. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate.
The TRANS-repressor sequence was determined by a computational analysis performed to minimize the interference with the genomic mRNAs and to maximize the base-pairing interaction to the CIS-repressing RNA.
We developed a circuit in order to test and characterize our T-Rex device:
The T-REX device is proposed as a universal and fast switch in synthetic gene circuits.
More details about our work in the Project section.
Acknowledgements
