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Team:Bologna
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[[Image:Ely9Copia.jpg|center|850px]] | [[Image:Ely9Copia.jpg|center|850px]] | ||
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| + | <div style="text-align:justify"> | ||
| + | = Project Summary = | ||
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'''Which is our idea?''' | '''Which is our idea?''' | ||
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| - | The project aims to implement a protein synthesis regulation system in | + | <html> |
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| - | + | The project aims to implement a <b>protein synthesis regulation system</b> in <i>Escherichia coli</i> that acts at translational level, regardless of the target gene. This <b>"general-purpose"</b> device allows a faster control of protein expression.<br>The device was named <b>T-Rex</b> (<b>T</b>rans <b>R</b>epressor of <b>Ex</b>pression). | |
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'''How can we achieve our goal?''' | '''How can we achieve our goal?''' | ||
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| + | T-Rex device consists of two new BioBricks: | ||
| + | <br><br> | ||
| + | <ul> | ||
| + | <li><font color="#000080"><b>CIS-repressing</b></font>, to be assembled upstream of the target protein coding sequence; it contains a ribosomal binding site; <font color="#228b22"><b>(RBS)</b></font> | ||
| + | </ul> | ||
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| + | <li><font color="#000080"><b>TRANS-repressor</b></font>, to be placed under the control of a different promoter; it is complementary to the CIS-repressing, and contains a <font color="#228b22"><b>RBS cover</b></font> in two versions of different length (4 and 7 nucleotides). | ||
| + | </ul> | ||
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| + | CIS-repressing and TRANS-repressor were designed using our [[Team:Bologna/Software#1|BASER]] tool. | ||
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| + | Transcription of the target gene produces a mRNA strand, starting with the Cis element, which is translated into proteins by ribosome. Trans’ promoter induction produces a transcript that binds with the Cis part. The <b>RNA duplex</b> prevents ribosome from binding to RBS, <b>repressing protein synthesis</b>. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate. | ||
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| + | <br><br> | ||
| + | [[Image:project3b.png|center|950px|]] | ||
| + | <br><br> | ||
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| - | + | '''How can we test the device?''' | |
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| - | + | In order to test and characterize our T-REX device, we developed the following genetic circuit:</font> | |
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| + | <br><br> | ||
| + | [[Image:circuit2.jpg|center|900px]] | ||
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The T-REX device is proposed as a universal and fast switch in synthetic gene circuits. | The T-REX device is proposed as a universal and fast switch in synthetic gene circuits. | ||
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More details about our work in the [[Team:Bologna/Project|Project]] section. | More details about our work in the [[Team:Bologna/Project|Project]] section. | ||
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= Acknowledgements = | = Acknowledgements = | ||
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* ''' [http://www.unibo.it/Portale/default.htm University of Bologna] ''' | * ''' [http://www.unibo.it/Portale/default.htm University of Bologna] ''' | ||
Revision as of 14:37, 21 October 2009
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Project Summary
Which is our idea?
The project aims to implement a protein synthesis regulation system in Escherichia coli that acts at translational level, regardless of the target gene. This "general-purpose" device allows a faster control of protein expression.
The device was named T-Rex (Trans Repressor of Expression).
How can we achieve our goal?
T-Rex device consists of two new BioBricks:
CIS-repressing and TRANS-repressor were designed using our [[Team:Bologna/Software#1|BASER]] tool.
Transcription of the target gene produces a mRNA strand, starting with the Cis element, which is translated into proteins by ribosome. Trans’ promoter induction produces a transcript that binds with the Cis part. The RNA duplex prevents ribosome from binding to RBS, repressing protein synthesis. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate.
How can we test the device?
In order to test and characterize our T-REX device, we developed the following genetic circuit:
The T-REX device is proposed as a universal and fast switch in synthetic gene circuits.
More details about our work in the Project section.
Acknowledgements
- Cultural Association San Sebastiano
