Team:Bologna

From 2009.igem.org

(Difference between revisions)
Line 51: Line 51:
</html>
</html>
<br><br>
<br><br>
-
[[Image:circuit2.jpg|center|900px|thumb|<center>Figure 2 - Genetic Circuit</center>]]
+
[[Image:circuit2ok.png|center|900px|thumb|<center>Figure 2 - Genetic Circuit</center>]]
<font face="Calibri" font size="4" color="#000000">
<font face="Calibri" font size="4" color="#000000">
<br><br>
<br><br>

Revision as of 17:18, 21 October 2009

ProvaBol2.png
HOME TEAM PROJECT SOFTWARE MODELING WET LAB PARTS HUMAN PRACTICE JUDGING CRITERIA




Ely9Copia.jpg


Project Summary


Which is our idea?

The project aims to design a new device to control the synthesis of a protein in Escherichia coli regardless of the protein to be controlled. This "general-purpose" standard device acts on translation to allow a faster silencing of protein expression if compared to standard regulated promoters. We named this device T-Rex (Trans Repressor of Expression).


How does T-Rex work?

The device consists of two new BioBricks:

  • CIS-repressing, to be assembled upstream of the target coding sequence.
  • TRANS-repressor, complementary to the CIS-repressing and placed under the control of a different promoter.

CIS-repressing and TRANS-repressor sequences were seeked by BASER software.

Transcription of the target gene produces a mRNA strand, starting with the Cis element, which is translated into proteins by ribosome (see Fig. 1, right panel). Trans’ promoter induction produces a transcript that binds with the Cis part. The RNA duplex prevents ribosome from binding to RBS, repressing protein synthesis. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate (see Fig. 1, left panel)

Figure 1 - T-Rex device



How can we test the device?


In order to test and characterize our T-REX device, we developed the following genetic circuit (Fig 2):

File:Circuit2ok.png
Figure 2 - Genetic Circuit



The T-REX device is proposed as a universal and fast switch in synthetic gene circuits.


More details about our work in the Project section.




Acknowledgements


  • [http://www.unibo.it/Portale/default.htm University of Bologna]


LogoUnibo.jpg








  • [http://serinar.criad.unibo.it Ser.In.Ar. Cesena]


Ser In Ar.jpg






  • Cultural Association San Sebastiano
SSebastiano.jpg











Locations of visitors to this page


Up