Team:Bologna

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More details about our work in the [[Team:Bologna/Project|Project]] section.
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More details about our work are reported in the [[Team:Bologna/Project|Project]] section.
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Revision as of 18:29, 21 October 2009

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Project Summary


Which is our idea?

The project aims to design a new device to control the synthesis of a protein in Escherichia coli regardless of the protein to be controlled. This "general-purpose" standard device acts on translation to allow a faster silencing of protein expression if compared to standard regulated promoters. We named this device T-Rex (Trans Repressor of Expression).


How does T-Rex work?

The device consists of two new BioBricks:

  • CIS-repressing, to be assembled upstream of the target coding sequence.
  • TRANS-repressor, complementary to the CIS-repressing and placed under the control of a different promoter.

CIS-repressing and TRANS-repressor sequences were seeked by BASER software.

Transcription of the target gene produces a mRNA strand, starting with the Cis element, which is translated into proteins by ribosome (see Fig. 1, right panel). Trans’ promoter induction produces a transcript that binds with the Cis part. The RNA duplex prevents ribosome from binding to RBS, repressing protein synthesis. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate (see Fig. 1, left panel)

Figure 1 - T-Rex device



How can we test the device?


In order to test and characterize our T-REX device, we developed the following genetic circuit (Fig 2):

Figure 2 - Genetic Circuit




More details about our work are reported in the Project section.




Acknowledgements



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  • Cultural Association San Sebastiano
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