Revision as of 10:07, 17 September 2009

HOME	TEAM	PROJECT	MODELING	WETLAB	DRY LAB	SUBMITTED PARTS	HUMAN PRACTICE

Our Team

Project Description

Which is our idea?

Our project aims the realization of a device for gene expression regulation at translation level, regardless of the target gene.

How can we achieve this?

We designed a 50bp non coding sequence (CIS - repressing) to be assembled upstream of the target gene RBS, while its complementary sequence (TRANS - repressor) is to be placed under the control of another promoter. The TRANS - repressing contains also a cover, that is a short sequence complementary to the RBS. When the TRANS-repressor element is present, it binds to the CIS-repressing, forming a RNA duplex and producing an obstruction that prevents the ribosome binding to the RBS.

What can we use it for?

After doing some tests to prove that the presence of CIS – repressing doesn't alter the cell normal activities, we are trying to realize some simple circuits in order to characterize our device. Several are the possible future applications, but at the moment we are aiming to oscillators and flip-flop memories.

More details about our work at the Project section.

@@ Line 53: / Line 53: @@
 <html>
-<script type="text/javascript">
+<center>
-/*
+<a href="http://www2.clustrmaps.com/counter/maps.php?url=https://2009.igem.org/Team:Bologna" id="clustrMapsLink"><img src="http://www2.clustrmaps.com/counter/index2.php?url=https://2009.igem.org/Team:Bologna" style="border:0px;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onerror="this.onerror=null; this.src='http://clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://clustrmaps.com';" />
-Simple Image Trail script- By JavaScriptKit.com
+</a>
-Visit http://www.javascriptkit.com for this script and more
-This notice must stay intact
-*/
-var trailimage=["http://www.asbafo.net/appoggio/ser_pul/logo.jpg", 11, 17] //image path, plus width and height
-var offsetfrommouse=[10,-20] //image x,y offsets from cursor position in pixels. Enter 0,0 for no offset
-var displayduration=0 //duration in seconds image should remain visible. 0 for always.
-if (document.getElementById || document.all)
-document.write('<div id="trailimageid" style="position:absolute;visibility:visible;left:0px;top:0px;width:1px;height:1px"><img src="'+trailimage[0]+'" border="0" width="'+trailimage[1]+'px" height="'+trailimage[2]+'px"></div>')
-function gettrailobj(){
-if (document.getElementById)
-return document.getElementById("trailimageid").style
-else if (document.all)
-return document.all.trailimagid.style
-}
-function truebody(){
-return (!window.opera && document.compatMode && document.compatMode!="BackCompat")? document.documentElement : document.body
-}
-function hidetrail(){
-gettrailobj().visibility="hidden"
-document.onmousemove=""
-}
-function followmouse(e){
-var xcoord=offsetfrommouse[0]
-var ycoord=offsetfrommouse[1]
-if (typeof e != "undefined"){
-xcoord+=e.pageX
-ycoord+=e.pageY
-}
-else if (typeof window.event !="undefined"){
-xcoord+=truebody().scrollLeft+event.clientX
-ycoord+=truebody().scrollTop+event.clientY
-}
-var docwidth=document.all? truebody().scrollLeft+truebody().clientWidth : pageXOffset+window.innerWidth-15
-var docheight=document.all? Math.max(truebody().scrollHeight, truebody().clientHeight) : Math.max(document.body.offsetHeight, window.innerHeight)
-if (xcoord+trailimage[1]+3>docwidth || ycoord+trailimage[2]> docheight)
-gettrailobj().display="none"
-else
-gettrailobj().display=""
-gettrailobj().left=xcoord+"px"
-gettrailobj().top=ycoord+"px"
-}
-document.onmousemove=followmouse
-if (displayduration>0)
-setTimeout("hidetrail()", displayduration*1000)
-</script>
 </html>