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Team:Bologna
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| - | The device consists of two new BioBricks | + | The device consists of two new BioBricks: |
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<li><font color="#000080"><b>TRANS-repressor</b></font>, complementary to the CIS-repressing and placed under the control of a different promoter. | <li><font color="#000080"><b>TRANS-repressor</b></font>, complementary to the CIS-repressing and placed under the control of a different promoter. | ||
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| - | CIS-repressing and TRANS-repressor were designed using our [[Team:Bologna/Software#1|BASER]] | + | CIS-repressing and TRANS-repressor were designed using our [[Team:Bologna/Software#1|BASER]] software. |
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| - | + | As shown in the figure, transcription of the target gene produces a mRNA strand, starting with the Cis element, which is translated into proteins by ribosome. Trans’ promoter induction produces a transcript that binds with the Cis part. The <b>RNA duplex</b> prevents ribosome from binding to RBS, <b>repressing protein synthesis</b>. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate. </font> | |
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'''How can we test the device?''' | '''How can we test the device?''' | ||
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Revision as of 16:40, 21 October 2009
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Project Summary
Which is our idea?
The project aims to design a new device to control the synthesis of a protein in Escherichia coli regardless of the protein to be controlled. This "general-purpose" standard device acts on translation to allow a faster silencing of protein expression if compared to standard regulated promoters. We named this device T-Rex (Trans Repressor of Expression).
How does T-Rex work?
The device consists of two new BioBricks:
CIS-repressing and TRANS-repressor were designed using our BASER software.
As shown in the figure, transcription of the target gene produces a mRNA strand, starting with the Cis element, which is translated into proteins by ribosome. Trans’ promoter induction produces a transcript that binds with the Cis part. The RNA duplex prevents ribosome from binding to RBS, repressing protein synthesis. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate. </font>
How can we test the device?
In order to test and characterize our T-REX device, we developed the following genetic circuit:
The T-REX device is proposed as a universal and fast switch in synthetic gene circuits.
More details about our work in the Project section.
Acknowledgements
- Cultural Association San Sebastiano
