Team:Bologna

From 2009.igem.org

Revision as of 11:42, 21 October 2009 by Andrea.samore (Talk | contribs)

ProvaBol2.png
HOME TEAM PROJECT SOFTWARE MODELING WET LAB PARTS HUMAN PRACTICE JUDGING CRITERIA


Ely9Copia.jpg


Project Summary

Which is our idea?

The project aims to implement a protein synthesis regulation system in Escherichia coli that acts at translational level regardless of the target gene to be downregulated. This "general-purpose" device could allow a faster control of protein expression. Our device was named T-Rex (Trans Repressor of Expression). It consists of two new BioBricks, i.e. the Trans-repressor and the Cis-repressing.


How can we achieve our goal?

The TRANS-repressor is a non-coding DNA sequence that acts as a silencer of the CIS-repressing mRNA. In fact, the Cis-repressing sequence includes a TRANS-repressor complementary region ending with a ribosome binding site (RBS). Moreover, it is assembled upstream of the target gene coding sequence. When the TRANS-repressor and the CIS-repressing mRNAs bind together, the RBS recognition by the ribosome is prevented. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate.
The TRANS-repressor sequence was determined by a computational analysis performed to minimize the interference with the genomic mRNAs and to maximize the base-pairing interaction to the CIS-repressing RNA.


Project3b.png


We developed the following genetic circuit in order to test and characterize our T-Rex device:


Circuit2.jpg




The T-REX device is proposed as a universal and fast switch in synthetic gene circuits.


More details about our work in the Project section.

Acknowledgements


LogoUnibo.jpg









Ser In Ar.jpg





  • Cultural Association San Sebastiano
SSebastiano.jpg









Locations of visitors to this page


Up