Team:Bologna/Parts

From 2009.igem.org

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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201004 BBa_K201004] 
 
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| Regulatory
 
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| Lac natural operator O2 with RFP
 
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201005 BBa_K201005] 
 
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| Lac natural operator O1 with RFP.
 
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201006 BBa_K201006] 
 
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| Regulatory
 
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| Lac natural operator Osym with RFP.
 
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K079031 BBa_K079031]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K079031 BBa_K079031]   
| Reporter
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K079032 BBa_K079032]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K079032 BBa_K079032]   
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<br>
 
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These parts should have been use in T-REX device, but we didn't succed in cloning them on standard plasimids.
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<br><br>
[https://2009.igem.org/Team:Bologna/Parts ''Up'']
[https://2009.igem.org/Team:Bologna/Parts ''Up'']

Revision as of 20:36, 21 October 2009

ProvaBol2.png
HOME TEAM PROJECT SOFTWARE MODELING WET LAB PARTS HUMAN PRACTICE JUDGING CRITERIA



Submitted Parts

NAME TYPE FRAGMENT PLASMID LENGTH (bp) SEQUENCED
1 BBa_K201000 Generator LacI repressed GFP generator with BBa_K201007 BBa_PSB1A2 986 -
2 BBa_K201001 Generator LacI repressed GFP generator with BBa_K201008 BBa_PSB1A2 986 Yes
3 BBa_K201002 Generator LacI repressor constitutive supplier BBa_PSB1A2 1351 Yes
4 BBa_K201002 Generator LacI repressor constitutive supplier BBa_PSB3K3 1351 -
5 BBa_K201003 Generator GFP generator BBa_PSB1A2 918 Yes
6 BBa_K201003 Generator GFP generator BBa_PSB3K3 918 -
7 BBa_K201004 Regulatory Lac natural operator O2 with RFP BBa_PSB1A2 733 -
8 BBa_K201005 Regulatory Lac natural operator O1 with RFP. BBa_PSB1A2 733 -
9 BBa_K201006 Regulatory Lac natural operator Osym with RFP. BBa_PSB1A2 732 Yes
10 BBa_K201007 Regulatory LacI repressed promoter BBa_J23118 BBa_PSB1A2 776 -
11 BBa_K201008 Regulatory LacI repressed promoter BBa_J23100 BBa_PSB1A2 776 -


Improved Parts

NAME TYPE FRAGMENT LENGTH (bp)
1 BBa_K079031 Reporter GFP reporter protein under the control of the BBa_J23118 promoter 957
2 BBa_K079032 Reporter GFP reporter protein under the control of the BBa_J23100 constitutive promoter 957


Added Parts

NAME TYPE FRAGMENT LENGTH (bp)
1 BBa_K201010 DNA Cis-Repressing sequence, to be used for T-REX device 105
2 BBa_K201011 DNA Trans-Repressor (4) sequence, to be used for T-REX device 103
3 BBa_K201012 DNA Trans-Repressor (7) sequence, to be used for T-REX device 100

These parts should have been use in T-REX device, but we didn't succed in cloning them on standard plasimids.

Up