Team:Bologna/Wetlab

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<a href="https://2009.igem.org/Team:Bologna/Characterization">PARTS CHARACTERIZATION <br><br> <img src="https://static.igem.org/mediawiki/2009/3/34/1429i13504psb1a2y100cgn170esp1%2C4_v1.png" width="115" height="75"></a></a></i></font>
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Circuit characterization.</i></font>
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!align="center"|[[Team:Bologna|<font color="#ffffff">HOME</font>]]
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!align="center"|[[Team:Bologna/Team|<font color="#ffffff">TEAM</font>]]
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!align="center"|[[Team:Bologna/Project|<font color="#ffffff">PROJECT</font>]]
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!align="center"|[[Team:Bologna/Modeling|<font color="#ffffff">MODELING</font>]]
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!align="center"|[[Team:Bologna/Wetlab|<font color="#ffffff">WETLAB</font>]]
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!align="center"|[[Team:Bologna/Lab-Notebook|<font color="#ffffff">LAB-NOTEBOOK</font>]]
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!align="center"|[[Team:Bologna/Software|<font color="#ffffff">DRY LAB</font>]]
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!align="center"|[[Team:Bologna/Parts|<font color="#ffffff">SUBMITTED PARTS</font>]]
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To test the ratio between the production of an high copy number plasmid (PSB1A2) and a low copy number one (PSB3K3), we assembled two circuits. The open loop GFP circuits are realized with a 1429 promotor and the standard biobrick I13504.
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<a href="https://2009.igem.org/Team:Bologna/T-REx Story">STORY OF A T-REX<br><br> <img src="https://static.igem.org/mediawiki/2009/f/f8/T-Rex.png" width="115" height="75"></a></a></i></font>
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The sad story of a little T-REX who couldn't find his home.</i></font>
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<a href="https://2009.igem.org/Team:Bologna/Lab-Notebook">NOTEBOOK <br><br> <img src="https://static.igem.org/mediawiki/2009/2/26/Diary123.jpg" width="115" height="75"></a></a></i></font>
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<font face="Times New Roman" font size="5"><i> Our wetlab notebook.</i></font>
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<a href="https://2009.igem.org/Team:Bologna/WetlabProtocols">PROTOCOLS <br><br> <img src="https://static.igem.org/mediawiki/2009/b/b3/Biohazard.png" width="105" height="90"></a></i></font>
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Here you can find all our experimental protocols.</i></font>
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PSB1A2 with high copy number plasmid and a low copy number were transformed in DH5alfa bacterial cells according to the standard protocol.
 
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One colony from each plate was picked up and let grow overnight in LB medium at 37°C. One milliliter for each of the two samples was collected by O/N cultures and spinned at 6000-8000 rpm for three minutes. The supernatant was harvested and the pellet resuspended. Slides were prepared for the fluorescence bacteria image acquisition.
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Finally, images were elaborated with the fluorescence visualization software and these are the results:
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|[[Image:1429i13504psb1a2y100cgn170esp1,4_v1.png|center|thumbnail|385 px|High copy number plasmid (PSB1A2)]]
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|[[Image:1429i13504psb3k3y100cgn170esp1,4_v1.png|center|thumbnail|385 px|Low copy number plasmid (PSB3K3)]]
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Latest revision as of 03:04, 22 October 2009

ProvaBol2.png
HOME TEAM PROJECT SOFTWARE MODELING WET LAB PARTS HUMAN PRACTICE JUDGING CRITERIA


PARTS CHARACTERIZATION

Circuit characterization.


STORY OF A T-REX

The sad story of a little T-REX who couldn't find his home.


NOTEBOOK

Our wetlab notebook.


PROTOCOLS

Here you can find all our experimental protocols.