Team:Brown/Notebook Recipes/SMMP


Making SMMP Medium ( Recovery Medium for Electroporated Staphylococcus epidermidis cells)

• Making 100ml of SMMP 1. 55.5 parts SMM Buffer a. Sucrose 18.8263g b. Maleic Acid 0.2554g c. MgCl2 6 H2O 0.4473g d. dd H2O 55ml

2. 4 parts 7% Panassy Broth a. 100% Panassy Broth 2.8ml b. ddH2O 40ml

3. 0.5 parts 10% BSA a. ddH2O 4.95ml ➢ Autoclave the solution. 10x BSA 50ul