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Team:Cambridge/Notebook/Week10
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Activator constructs 50, 51, 52 and the part B0015 were mini-prepped from the overnight culture and run on a gel for extraction and purification. Ligations between each of the activators and B0015 were then carried out. | Activator constructs 50, 51, 52 and the part B0015 were mini-prepped from the overnight culture and run on a gel for extraction and purification. Ligations between each of the activators and B0015 were then carried out. | ||
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Revision as of 09:50, 24 September 2009
Categories :
Project :
-
Overview
Sensitivity Tuner
--- Characterisation
--- Modelling
Colour Generators
--- Carotenoids (Orange/Red)
--- Melanin (Brown)
--- Violacein (Purple/Green)
The Future
Safety
Notebook :
Team Logistics :
Week 10
Monday
Wet Work
Threshold Devices
Confirmed successful transfer of 40 (pBad followed by GFP) into the low copy plasmid pSB3K3. Transformed 40 in pSB3K3 into arabinose strain.
Confirmed successful construction of pBad + activator (50, 51, and 52) + B0015.
Transformation of TOP10 with activator constructs 50, 51, 52 and the part B0015 in preparation for ligation to pigment parts.
Tuesday
Dry Work
Wet Work
Transformants from yesterday put into overnight culture.
Wednesday
Wet Work
Activator constructs 50, 51, 52 and the part B0015 were mini-prepped from the overnight culture and run on a gel for extraction and purification. Ligations between each of the activators and B0015 were then carried out.
Thursday
Friday
