Parts Submitted to Registry

Registry Code	Type	Sequence Description / Notes	Length
	Coding	Produces dark brown pigment output. The gene (melA) codes for a tyrosinase which produces the pigment from L-tyrosine. Production of the pigment requires the addition of copper and L-tyrosine supplements (the copper acts as a cofactor for the gene product) but no other precursors. The BioBrick sequence includes the native ribosome binding site.	1844 bp
	Composite	CrtEBI with rbs. This Composite Biobrick is created by standard assembly of 3 basic Biobricks coding for enzymes CrtE, CrtB and CrtI (each with rbs). Together, enzymes CrtE, CrtB and CrtI convert colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene). The red lycopene pigment can then be used as a coloured signal output, e.g. for biosensors.	3385 bp
Generator	CrtEBI under constitutive promoter. This Biobrick is created by putting enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274100 under constitutive promoter R0011. Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone).	3448 bp
Generator	CrtEBI under pBad promoter. This Biobrick is created by putting enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274100 under arabinose-induced promoter I0500. Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone).	1844 bp

Team:Cambridge/Parts