Team:Chiba

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:::::<Font Size="7">'''''E''.coli Time Manager '''</Font>  <html><a href="http://www.chiba-u.ac.jp/e/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/5/5f/Logo_chiba-u.gif" align="right" width="250"></a></html>
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{| style="color:white;" cellpadding="2" cellspacing="0" border="0" width="100%" align="center" class="menu" |align="center"
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!align="center"|[[Team:Chiba|Home]]
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!align="center"|[[Team:Chiba/Project|The Project]]
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!align="center"|[[Team:Chiba/Team|The Team]]
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!align="center"|[[Team:Chiba/Parts|Parts]]
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!align="center"|[[Team:Chiba/Reference|Reference]]
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!align="center"|[[Team:Chiba/Notebook|Notebook]]
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!align="center"|[[Team:Chiba/Notebook/protocol|Protocols]]
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!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
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!align="center"|[[Team:Chiba/Contact|Contact]]
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'''[[Team:Chiba/Project|The Project]]'''
'''[[Team:Chiba/Project|The Project]]'''
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*[[Team:Chiba/Project#Introduction|Introduction]]
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'''1,''' [[Team:Chiba/Project#Introduction|Introduction]]
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*[[Team:Chiba/Project#Project_Design|Project Design]]
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'''2,''' [[Team:Chiba/Project#Project_Design|Project Design]]
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*[[Team:Chiba/Project#Conclusions|Conclusions]]
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'''3,''' [[Team:Chiba/Project#Experiments,_Results_&_Discussion|Experiments, Results & Discussion]]
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'''[[Team:Chiba/Project/Delay_Switch|Delay Switch]]'''
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*[[Team:Chiba/Project/Delay_Switch#Experiments_(2008)|Experiments 2008]]
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3-1, [[Team:Chiba/Project#Making_LuxR_Mutants|Making LuxR Mutants]]
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*[[Team:Chiba/Project/Delay_Switch#Results_(2008)|Results 2008]]
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3-2, [[Team:Chiba/Project#Characterization_of_LuxR_Mutants|Characterization of LuxR Mutants]]
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*[[Team:Chiba/Project/Delay_Switch#Experiments_(2009)|Experiments 2009]]
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3-3, [[Team:Chiba/Project#Demonstration|Demonstration]]
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*[[Team:Chiba/Project/Delay_Switch#Results_(2009)|Results 2009]]
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'''5,''' [[Team:Chiba/Project#Conclusions|Conclusions]]
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'''[[Team:Chiba/Project/Parallel|Parallel Activation]]'''
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*[[Team:Chiba/Modeling|Modeling]]
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*[[Team:Chiba/Project/Parallel#Experiments|Experiments]]
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*[[Team:Chiba/Project/Parallel#Results|Results]]
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'''[[Team:Chiba/Project/Series|Series Activation]]'''
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*[[Team:Chiba/Modeling|Modeling]]
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*[[Team:Chiba/Project/Series#Experiments|Experiments]]
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*[[Team:Chiba/Project/Series#Results|Results]]
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<Font Size="6">''E''.coli Time Manager Since 2008</Font>  <html><a href="http://www.chiba-u.ac.jp/e/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/5/5f/Logo_chiba-u.gif" align="right" width="250"></a></html>
 
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{| style="color:white;" cellpadding="2" cellspacing="0" border="0" width="100%" align="center" class="menu" |align="center"
 
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!align="center"|[[Team:Chiba|Home]]
 
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!align="center"|[[Team:Chiba/Team|The Team]]
 
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!align="center"|[[Team:Chiba/Parts|Parts]]
 
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!align="center"|[[Team:Chiba/Reference|Reference]]
 
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!align="center"|[[Team:Chiba/Notebook|Notebook]]
 
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!align="center"|[[Team:Chiba/Notebook/protocol|Protocols]]
 
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!align="center"|[[Team:Chiba/Links|Links]]
 
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!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
 
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!align="center"|[[Team:Chiba/Contact|Contact]]
 
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__NOTOC__
__NOTOC__
'''Welcome to the Chiba team Wiki for iGEM 2009!'''
'''Welcome to the Chiba team Wiki for iGEM 2009!'''
[[Image:Team-Chiba-members.JPG|right|frame|Team Photo]]
[[Image:Team-Chiba-members.JPG|right|frame|Team Photo]]
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I’ll introduce Team Chiba to you.  Team Chiba joined iGEM in 2006. Its members belong to The Dep. of Chemistry and so we did not know much biology.  The members therefore had to start from studying the basics of biology. This was the characteristic, but what I believe to be the biggest weak point for the Team.
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We introduce Team Chiba to you.  Team Chiba joined iGEM in 2006. Its members belong to The Dep. of Chemistry and so we did not know much biology.  The members therefore had to start from studying the basics of biology. This was the characteristic, but what I believe to be the biggest weak point for the Team.
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This year, However, Team Chiba has a member who belongs to the Dep. of biology! He is nothing but an undergraduate student but I expect that the energy of our team will be made better by his existence.
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This year, However, Team Chiba has a member who belongs to the Dep. of biology! He is nothing but an undergraduate student but we expect that the energy of our team will be made better by his existence.
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This years project is making the most of last year's project. Last year, we tried to devise a series of delay-switches by designing a "switching consortia". We got limited, in success in generating delay swithces, but I think that there is a great deal of room for improvement.
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This year's project is making a new version of last year's project. Last year, we tried to devise a series of delay-switches by designing a "switching consortia". We got limited, in success in generating delay swithces, but we think that there is a great deal of room for improvement.
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The Registration of our team is very late, in June, and our team has only 5 people. I don't know what will become of our team, but I think the best for our team that all members our team enjoy to the full! Thank you. (Asia iGEM Workshop)
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The Registration of our team is very late, in June, and our team has only 5 people. We don't know what will become of our team, but we think the best for our team that all members our team enjoy to the full! Thank you. (Asia iGEM Workshop)
== Project - ''E''. coli Time Manager Since 2008 ==
== Project - ''E''. coli Time Manager Since 2008 ==
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We are constructing delay switches to control/preset the timing of target gene expression.
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::Since 2008, we have been constructing the bacteria timer that "work together".
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The mechanism is very simple; (1) the "Transmitter cells" generates the signal molecules, whose concentration gradually increases, (2) when it reaches a certain level, the "Receiver cells" switch on the expression of any given genes. Precise control of the time of delay of this entire process, one can pre-set the time of expression of genetic functions in a predicable manner.
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Our project uses two classes of bacteria: senders and receivers. Senders produce signaling molecules, and receivers are activated only after a particular concentration of this molecule is reached. The combinatorial use of senders/receivers allows us to make a‘switching consortium’which activates different genes at the preset times.
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By using Asyl-Homoserine Lactones(AHLs) that can freely pass through the cell membrane as signal molecules, the time can be shared, in real time, by all cells within the pot. This way, receiver (timer) cells would take the action all at once in right timing, minimizing the distribution in each cell's response time.
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This year, we are trying to make a platform for generating an animated pictures using series of new timer cells we have constructed.
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私たちが2008年から取り組んでいるのは、細菌同士の化学通信機構を利用した「集団で発揮する」タイマー機能です。具体的なシステムは「送信機細胞」がつくるシグナル分子を「受信機細胞」が感知することで色変化を起こすというものです。シグナル分子がじわじわと増えてゆき、遂に受信細胞が感じる濃度に達した時に色変化が起きます。この蓄積にかかる時間がタイマー時間となるわけです。
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私たちは、細胞膜を自由に通過できる分子(アシル化ホモセリンラクトン群)をシグナル分子として用いることで系内の何万もの細胞に「感じている時間」を共有させることができるよう工夫をしました。これによって、細胞達はばらばらに「ずれて」光り始めるのではなく、集団として一糸乱れず同時にアクションを起こすことができます。この「時間合わせ」機能を内蔵する新しいタイマー細胞を用いて、数段階に機能するタイマーを作るのが今年の目的です。
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<A HREF="http://kserv.jp/"><IMG SRC = "http://chem.tf.chiba-u.jp/igem/img/knowledge.jpg" ALT = "" width="180px"></A> 
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<A HREF="http://www.neo-morgan.com/"><IMG SRC = "http://chem.tf.chiba-u.jp/igem/img/neomorgan.png" ALT = ""></A> 
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<A HREF="http://www.greiner-bio-one.co.jp/top.html"><IMG SRC = "http://chem.tf.chiba-u.jp/igem/img/greiner.gif" ALT = ""></A><br><br>
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<a href="http://www.keyence.co.jp/"><img src="http://chem.tf.chiba-u.jp/igem/img/keyence.gif"></a>  
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<a href="http://www.cosmobio.co.jp/"><img src="http://chem.tf.chiba-u.jp/igem/img/cosmo.jpg"></a><br><br>
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<A HREF="http://www.stratagene.com/homepage/"><IMG SRC = "http://chem.tf.chiba-u.jp/igem/img/stratagene.gif" ALT = "" width="180px"></A><br><br>
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<a href="http://chiba-kougaku-dosokai.jp/"><img src="http://chem.tf.chiba-u.jp/igem/img/kougaku.gif" width="180px"></a> 
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<A HREF="http://www.chiba-u.ac.jp/"><IMG SRC = "http://chem.tf.chiba-u.jp/igem/img/chiba-u.gif" ALT = "" width="180px"></A> <br><br>
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<a href="http://pulsar.tc.chiba-u.jp/~dosokai/" style="color:#666666;">Suzuna-no-kai, Chiba Univ.</a>
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Latest revision as of 03:57, 22 October 2009

E.coli Time Manager


The Project

1, Introduction

2, Project Design

3, Experiments, Results & Discussion

3-1, Making LuxR Mutants

3-2, Characterization of LuxR Mutants

3-3, Demonstration

5, Conclusions

Welcome to the Chiba team Wiki for iGEM 2009!

Team Photo

We introduce Team Chiba to you. Team Chiba joined iGEM in 2006. Its members belong to The Dep. of Chemistry and so we did not know much biology. The members therefore had to start from studying the basics of biology. This was the characteristic, but what I believe to be the biggest weak point for the Team.

This year, However, Team Chiba has a member who belongs to the Dep. of biology! He is nothing but an undergraduate student but we expect that the energy of our team will be made better by his existence.

This year's project is making a new version of last year's project. Last year, we tried to devise a series of delay-switches by designing a "switching consortia". We got limited, in success in generating delay swithces, but we think that there is a great deal of room for improvement.

The Registration of our team is very late, in June, and our team has only 5 people. We don't know what will become of our team, but we think the best for our team that all members our team enjoy to the full! Thank you. (Asia iGEM Workshop)

Project - E. coli Time Manager Since 2008

Since 2008, we have been constructing the bacteria timer that "work together".

The mechanism is very simple; (1) the "Transmitter cells" generates the signal molecules, whose concentration gradually increases, (2) when it reaches a certain level, the "Receiver cells" switch on the expression of any given genes. Precise control of the time of delay of this entire process, one can pre-set the time of expression of genetic functions in a predicable manner. By using Asyl-Homoserine Lactones(AHLs) that can freely pass through the cell membrane as signal molecules, the time can be shared, in real time, by all cells within the pot. This way, receiver (timer) cells would take the action all at once in right timing, minimizing the distribution in each cell's response time. This year, we are trying to make a platform for generating an animated pictures using series of new timer cells we have constructed.

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Suzuna-no-kai, Chiba Univ.