Team:Chiba

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:::::<Font Size="7">'''''E''.coli Time Manager '''</Font>  <html><a href="http://www.chiba-u.ac.jp/e/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/5/5f/Logo_chiba-u.gif" align="right" width="250"></a></html>
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{| style="color:white;" cellpadding="2" cellspacing="0" border="0" width="100%" align="center" class="menu" |align="center"
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!align="center"|[[Team:Chiba|Home]]
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!align="center"|[[Team:Chiba/Project|The Project]]
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!align="center"|[[Team:Chiba/Team|The Team]]
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!align="center"|[[Team:Chiba/Parts|Parts]]
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!align="center"|[[Team:Chiba/Reference|Reference]]
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!align="center"|[[Team:Chiba/Notebook|Notebook]]
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!align="center"|[[Team:Chiba/Notebook/protocol|Protocols]]
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!align="center"|[[Team:Chiba/Links|Links]]
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!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
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!align="center"|[[Team:Chiba/Contact|Contact]]
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'''[[Team:Chiba/Project|The Project]]'''
'''[[Team:Chiba/Project|The Project]]'''
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*[[Team:Chiba/Project#Introduction|Introduction]]
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'''1,''' [[Team:Chiba/Project#Introduction|Introduction]]
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*[[Team:Chiba/Project#Project_Design|Project Design]]
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'''2,''' [[Team:Chiba/Project#Project_Design|Project Design]]
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*[[Team:Chiba/Project#Conclusions|Conclusions]]
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'''3,''' [[Team:Chiba/Project#Experiments,_Results_&_Discussion|Experiments, Results & Discussion]]
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'''[[Team:Chiba/Project/Delay_Switch|Delay Switch]]'''
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*[[Team:Chiba/Project/Delay_Switch#Experiments_(2008)|Experiments 2008]]
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3-1, [[Team:Chiba/Project#Making_LuxR_Mutants|Making LuxR Mutants]]
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*[[Team:Chiba/Project/Delay_Switch#Results_(2008)|Results 2008]]
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3-2, [[Team:Chiba/Project#Characterization_of_LuxR_Mutants|Characterization of LuxR Mutants]]
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*[[Team:Chiba/Project/Delay_Switch#Experiments_(2009)|Experiments 2009]]
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3-3, [[Team:Chiba/Project#Demonstration|Demonstration]]
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*[[Team:Chiba/Project/Delay_Switch#Results_(2009)|Results 2009]]
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'''5,''' [[Team:Chiba/Project#Conclusions|Conclusions]]
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'''[[Team:Chiba/Project/Parallel|Parallel Activation]]'''
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*[[Team:Chiba/Modeling|Modeling]]
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*[[Team:Chiba/Project/Parallel#Experiments|Experiments]]
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*[[Team:Chiba/Project/Parallel#Results|Results]]
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'''[[Team:Chiba/Project/Series|Series Activation]]'''
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*[[Team:Chiba/Modeling|Modeling]]
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*[[Team:Chiba/Project/Series#Experiments|Experiments]]
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*[[Team:Chiba/Project/Series#Results|Results]]
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<Font Size="6">''E''.coli Time Manager Since 2008</Font>  <html><a href="http://www.chiba-u.ac.jp/e/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/5/5f/Logo_chiba-u.gif" align="right" width="250"></a></html>
 
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{| style="color:white;" cellpadding="2" cellspacing="0" border="0" width="100%" align="center" class="menu" |align="center"
 
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!align="center"|[[Team:Chiba|Home]]
 
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!align="center"|[[Team:Chiba/Team|The Team]]
 
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!align="center"|[[Team:Chiba/Parts|Parts]]
 
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!align="center"|[[Team:Chiba/Reference|Reference]]
 
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!align="center"|[[Team:Chiba/Notebook|Notebook]]
 
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!align="center"|[[Team:Chiba/Notebook/protocol|Protocols]]
 
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!align="center"|[[Team:Chiba/Links|Links]]
 
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!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
 
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!align="center"|[[Team:Chiba/Contact|Contact]]
 
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__NOTOC__
__NOTOC__
'''Welcome to the Chiba team Wiki for iGEM 2009!'''
'''Welcome to the Chiba team Wiki for iGEM 2009!'''
[[Image:Team-Chiba-members.JPG|right|frame|Team Photo]]
[[Image:Team-Chiba-members.JPG|right|frame|Team Photo]]
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I’ll introduce Team Chiba to you.  Team Chiba joined iGEM in 2006. Its members belong to The Dep. of Chemistry and so we did not know much biology.  The members therefore had to start from studying the basics of biology. This was the characteristic, but what I believe to be the biggest weak point for the Team.
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We introduce Team Chiba to you.  Team Chiba joined iGEM in 2006. Its members belong to The Dep. of Chemistry and so we did not know much biology.  The members therefore had to start from studying the basics of biology. This was the characteristic, but what I believe to be the biggest weak point for the Team.
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This year, However, Team Chiba has a member who belongs to the Dep. of biology! He is nothing but an undergraduate student but I expect that the energy of our team will be made better by his existence.
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This year, However, Team Chiba has a member who belongs to the Dep. of biology! He is nothing but an undergraduate student but we expect that the energy of our team will be made better by his existence.
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This years project is making the most of last year's project. Last year, we tried to devise a series of delay-switches by designing a "switching consortia". We got limited, in success in generating delay swithces, but I think that there is a great deal of room for improvement.
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This year's project is making a new version of last year's project. Last year, we tried to devise a series of delay-switches by designing a "switching consortia". We got limited, in success in generating delay swithces, but we think that there is a great deal of room for improvement.
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The Registration of our team is very late, in June, and our team has only 5 people. I don't know what will become of our team, but I think the best for our team that all members our team enjoy to the full! Thank you. (Asia iGEM Workshop)
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The Registration of our team is very late, in June, and our team has only 5 people. We don't know what will become of our team, but we think the best for our team that all members our team enjoy to the full! Thank you. (Asia iGEM Workshop)
== Project - ''E''. coli Time Manager Since 2008 ==
== Project - ''E''. coli Time Manager Since 2008 ==
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What we working since 2008 is to make a function of "Demonstrate its role in a group" timer.A concrete system is the one of causing the color change because "Receiver cell" perceives the signal molecule that "Transmitter cell" makes. The color change occurs when the signal molecule increases gradually and it reaches the density that the reception cell finally feels.The term that necessary to this accumulation becomes the time of the timer. 
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::Since 2008, we have been constructing the bacteria timer that "work together".
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The mechanism is very simple; (1) the "Transmitter cells" generates the signal molecules, whose concentration gradually increases, (2) when it reaches a certain level, the "Receiver cells" switch on the expression of any given genes. Precise control of the time of delay of this entire process, one can pre-set the time of expression of genetic functions in a predicable manner.
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We devised to make "Felt time" shared in tens of thousands of cells in the system by using the molecule that was able to pass the cell membrane freely(Asyl-Homoserine Lactone(AHL) groups) as a signal molecule.As a result, cells can act the action at the same time in good order in splinters as the group, not begin to be "Shift" shines.
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By using Asyl-Homoserine Lactones(AHLs) that can freely pass through the cell membrane as signal molecules, the time can be shared, in real time, by all cells within the pot. This way, receiver (timer) cells would take the action all at once in right timing, minimizing the distribution in each cell's response time.
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This year, we are trying to make a platform for generating an animated pictures using series of new timer cells we have constructed.
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This year's purpose is to to make the timer that functions to several step floor by using a new timer cell with built-in this "Match at time" function.
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We challenge to making animation with this timer.  
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<A HREF="http://kserv.jp/"><IMG SRC = "http://chem.tf.chiba-u.jp/igem/img/knowledge.jpg" ALT = "" width="180px"></A> 
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<A HREF="http://www.neo-morgan.com/"><IMG SRC = "http://chem.tf.chiba-u.jp/igem/img/neomorgan.png" ALT = ""></A> 
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<A HREF="http://www.greiner-bio-one.co.jp/top.html"><IMG SRC = "http://chem.tf.chiba-u.jp/igem/img/greiner.gif" ALT = ""></A><br><br>
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<a href="http://www.keyence.co.jp/"><img src="http://chem.tf.chiba-u.jp/igem/img/keyence.gif"></a>  
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<a href="http://www.cosmobio.co.jp/"><img src="http://chem.tf.chiba-u.jp/igem/img/cosmo.jpg"></a><br><br>
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<A HREF="http://www.stratagene.com/homepage/"><IMG SRC = "http://chem.tf.chiba-u.jp/igem/img/stratagene.gif" ALT = "" width="180px"></A><br><br>
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<a href="http://chiba-kougaku-dosokai.jp/"><img src="http://chem.tf.chiba-u.jp/igem/img/kougaku.gif" width="180px"></a> 
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<A HREF="http://www.chiba-u.ac.jp/"><IMG SRC = "http://chem.tf.chiba-u.jp/igem/img/chiba-u.gif" ALT = "" width="180px"></A> <br><br>
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<a href="http://pulsar.tc.chiba-u.jp/~dosokai/" style="color:#666666;">Suzuna-no-kai, Chiba Univ.</a>
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Latest revision as of 03:57, 22 October 2009

E.coli Time Manager


The Project

1, Introduction

2, Project Design

3, Experiments, Results & Discussion

3-1, Making LuxR Mutants

3-2, Characterization of LuxR Mutants

3-3, Demonstration

5, Conclusions

Welcome to the Chiba team Wiki for iGEM 2009!

Team Photo

We introduce Team Chiba to you. Team Chiba joined iGEM in 2006. Its members belong to The Dep. of Chemistry and so we did not know much biology. The members therefore had to start from studying the basics of biology. This was the characteristic, but what I believe to be the biggest weak point for the Team.

This year, However, Team Chiba has a member who belongs to the Dep. of biology! He is nothing but an undergraduate student but we expect that the energy of our team will be made better by his existence.

This year's project is making a new version of last year's project. Last year, we tried to devise a series of delay-switches by designing a "switching consortia". We got limited, in success in generating delay swithces, but we think that there is a great deal of room for improvement.

The Registration of our team is very late, in June, and our team has only 5 people. We don't know what will become of our team, but we think the best for our team that all members our team enjoy to the full! Thank you. (Asia iGEM Workshop)

Project - E. coli Time Manager Since 2008

Since 2008, we have been constructing the bacteria timer that "work together".

The mechanism is very simple; (1) the "Transmitter cells" generates the signal molecules, whose concentration gradually increases, (2) when it reaches a certain level, the "Receiver cells" switch on the expression of any given genes. Precise control of the time of delay of this entire process, one can pre-set the time of expression of genetic functions in a predicable manner. By using Asyl-Homoserine Lactones(AHLs) that can freely pass through the cell membrane as signal molecules, the time can be shared, in real time, by all cells within the pot. This way, receiver (timer) cells would take the action all at once in right timing, minimizing the distribution in each cell's response time. This year, we are trying to make a platform for generating an animated pictures using series of new timer cells we have constructed.

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Suzuna-no-kai, Chiba Univ.