Team:Chiba/Notebook/Calendar/12 September 2009
From 2009.igem.org
(Difference between revisions)
(→Lab Notebook) |
(→Check of Sender's AHL generation (2)) |
||
Line 8: | Line 8: | ||
== Check of Sender's AHL generation (2) == | == Check of Sender's AHL generation (2) == | ||
+ | ===08:15 Main culture=== | ||
- | + | ===Measuring OD=== | |
- | + | *13:35 OD<sub>600</sub>=1.02 | |
- | + | *14:45 OD<sub>600</sub>=1.42 | |
- | + | *16:20 OD<sub>600</sub>=1.64 | |
- | + | *18:30 OD<sub>600</sub>=1.72 | |
- | 13:35 | + | |
- | + | ||
- | OD<sub>600</sub>=1.02 | + | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | 14:45 | + | |
- | + | ||
- | OD<sub>600</sub>=1.42 | + | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | 16:20 | + | |
- | + | ||
- | OD<sub>600</sub>=1.64 | + | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | 18:30 | + | |
- | + | ||
- | OD<sub>600</sub>=1.72 | + | |
'''We stopped culture and the experiments.''' | '''We stopped culture and the experiments.''' |
Revision as of 18:28, 23 September 2009
(11_September_2009 <|>13_September_2009)
Contents |
Check of Sender's AHL generation (2)
08:15 Main culture
Measuring OD
- 13:35 OD600=1.02
- 14:45 OD600=1.42
- 16:20 OD600=1.64
- 18:30 OD600=1.72
We stopped culture and the experiments.
Check of Sender's AHL generation (3)
- Prior culture
19:00
We did prior culture using glycerol stock(XL10G, plac-LuxI, +LVA) .
E. coli Painting
- Results of the experiment at 9, September.