Team:Chiba/Notebook/Calendar/13 September 2009

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=== Check of Sender's AHL generation (3) ===
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=== Check of Sender's AHL generation ===
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Revision as of 16:17, 14 September 2009

>Go to the Notebook page

(12_September_2009 <|>14_September_2009)

Contents

Check of Sender's AHL generation (3)

  • Main culture

7:10-


  • Measuring OD

12:20

OD600=1.98


  • Wash

12:40

First, we dispensed culture solution about 12.5 mL to each 10 centrifuging tubes.


12:45

Next, we centrifuged samples 3 times(rpm=3000, 5 min, 20 degrees Celsius).


  • Centrifugation

14:35

We centrifuged one of samples, and cultured the others at 37 degrees Celsius.

And then,



Check of Sender's AHL generation

18:05 Start


18:35


19:05


19:35

20:05

20:35

21:05


21:35


22:05