Team:Chiba/Notebook/Calendar/22 September 2009
From 2009.igem.org
(Difference between revisions)
(→To judge character of LuxR mutants) |
(→26:35) |
||
Line 94: | Line 94: | ||
Image:Chiba_2209_2635_1000.jpg|(3 )AHL 1000nM | Image:Chiba_2209_2635_1000.jpg|(3 )AHL 1000nM | ||
</gallery> | </gallery> | ||
+ | |||
+ | == ''E''. coli Painting (2) == |
Revision as of 09:15, 23 September 2009
(21_September_2009 <|>23_September_2009)
Contents |
To judge character of LuxR mutants (1)
Yesterday's operation is here.
Today's operation
11:00-
We transplanted E.coli by 48 pins to NC filter and cultured it.
22:30
We transplanted E.coli, which has been cultured on NC filter, to solid medium which contains each concentration of AHL.
AHL concentration is : 0, 10, and 1000 nM
We decided this time is T=0 and observed condition of fluorescence every 30 min.
Pictures are here.