Team:Chiba/Notebook/Calendar/22 September 2009

From 2009.igem.org

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We decided this time is T=0 and observed condition of fluorescence every 30 min.
We decided this time is T=0 and observed condition of fluorescence every 30 min.
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'''Mutants' Location'''
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<table width="300" border="1" cellpadding="0"  cellspacing="0" bordercolor="#000000"><tr>
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<td width="150">Wild Type x 3 well</td>
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<td width="150">Mutant 8 x 3 well</td>
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</tr>
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<tr>
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<td>Mutant 1</td>
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<td>Mutant 9</td>
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</tr>
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<tr>
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<td>Mutant 2</td>
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<td>Mutant 10</td>
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</tr>
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<tr>
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<td>Mutant 3</td>
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<td>Mutant 11</td>
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</tr>
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<tr>
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<td>Mutant 4</td>
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<td>Mutant L1</td>
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</tr>
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<tr>
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<td>Mutant 5</td>
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<td>Mutant L2</td>
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</tr>
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<tr>
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<td>Mutant 6</td>
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<td>Negative Control</td>
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</tr>
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<tr>
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<td>Mutant 7</td>
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<td>(Nothing)</td>
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</tr>
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</table>
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'''Pictures are here.'''
'''Pictures are here.'''

Revision as of 10:45, 23 September 2009

>Go to the Notebook page

(21_September_2009 <|>23_September_2009)


Contents

To judge character of LuxR mutants (1)

Yesterday's operation is here.


Today's operation


11:00-

We transplanted E.coli by 48 pins to NC filter and cultured it.


22:30

We transplanted E.coli, which has been cultured on NC filter, to solid medium which contains each concentration of AHL.

AHL concentration is : 0, 10, and 1000 nM


We decided this time is T=0 and observed condition of fluorescence every 30 min.


Mutants' Location

Wild Type x 3 well Mutant 8 x 3 well
Mutant 1 Mutant 9
Mutant 2 Mutant 10
Mutant 3 Mutant 11
Mutant 4 Mutant L1
Mutant 5 Mutant L2
Mutant 6 Negative Control
Mutant 7 (Nothing)


Pictures are here.

observed condition of fluorescence every 30 min

22:35 Start

(1) AHL 0 nM

(2) AHL 10 nM

(3 )AHL 1000nM

23:05

(1) AHL 0 nM

(2) AHL 10 nM

(3 )AHL 1000nM

23:35

(1) AHL 0 nM

(2) AHL 10 nM

(3 )AHL 1000nM

24:05

(1) AHL 0 nM

(2) AHL 10 nM

(3 )AHL 1000nM

24:35

(1) AHL 0 nM

(2) AHL 10 nM

(3 )AHL 1000nM

25:05

(1) AHL 0 nM

(2) AHL 10 nM

(3 )AHL 1000nM

25:35

(1) AHL 0 nM

(2) AHL 10 nM

(3 )AHL 1000nM

26:05

(1) AHL 0 nM

(2) AHL 10 nM

(3)AHL 1000nM

26:35

(1) AHL 0 nM

(2) AHL 10 nM

(3 )AHL 1000nM

E. coli Painting (2)

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