"
Team:Chiba/Notebook/Calendar/22 September 2009
From 2009.igem.org
| Line 6: | Line 6: | ||
| - | == To judge character of LuxR mutants (1) == | + | == To judge character of LuxR mutants (1)-2 == |
Yesterday's operation is [https://2009.igem.org/Team:Chiba/Notebook/Calendar/21_September_2009 here]. | Yesterday's operation is [https://2009.igem.org/Team:Chiba/Notebook/Calendar/21_September_2009 here]. | ||
| Line 135: | Line 135: | ||
== ''E''. coli Painting (2) == | == ''E''. coli Painting (2) == | ||
| + | |||
| + | == To judge character of LuxR mutants (2)-1 == | ||
| + | We poured 1 mL of LB-Amp, Cm liquid medium in 96 deep well and added glycerol stocks. | ||
| + | |||
| + | |||
| + | 11:45- | ||
| + | |||
| + | We cultured and shook it at 37 degrees Celsius. | ||
Revision as of 10:50, 23 September 2009
(21_September_2009 <|>23_September_2009)
Contents |
To judge character of LuxR mutants (1)-2
Yesterday's operation is here.
Today's operation
11:00-
We transplanted E.coli by 48 pins to NC filter and cultured it.
22:30
We transplanted E.coli, which has been cultured on NC filter, to solid medium which contains each concentration of AHL.
AHL concentration is : 0, 10, and 1000 nM
We decided this time is T=0 and observed condition of fluorescence every 30 min.
Mutants' Location
| Wild Type x 3 well | Mutant 8 x 3 well |
| Mutant 1 | Mutant 9 |
| Mutant 2 | Mutant 10 |
| Mutant 3 | Mutant 11 |
| Mutant 4 | Mutant L1 |
| Mutant 5 | Mutant L2 |
| Mutant 6 | Negative Control |
| Mutant 7 | (Nothing) |
Pictures are here.
observed condition of fluorescence every 30 min
22:35 Start
 (1) AHL 0 nM |
 (2) AHL 10 nM |
 (3 )AHL 1000nM |
23:05
 (1) AHL 0 nM |
 (2) AHL 10 nM |
 (3 )AHL 1000nM |
23:35
 (1) AHL 0 nM |
 (2) AHL 10 nM |
 (3 )AHL 1000nM |
24:05
 (1) AHL 0 nM |
 (2) AHL 10 nM |
 (3 )AHL 1000nM |
24:35
 (1) AHL 0 nM |
 (2) AHL 10 nM |
 (3 )AHL 1000nM |
25:05
 (1) AHL 0 nM |
 (2) AHL 10 nM |
 (3 )AHL 1000nM |
25:35
 (1) AHL 0 nM |
 (2) AHL 10 nM |
 (3 )AHL 1000nM |
26:05
 (1) AHL 0 nM |
 (2) AHL 10 nM |
 (3)AHL 1000nM |
26:35
 (1) AHL 0 nM |
 (2) AHL 10 nM |
 (3 )AHL 1000nM |
E. coli Painting (2)
To judge character of LuxR mutants (2)-1
We poured 1 mL of LB-Amp, Cm liquid medium in 96 deep well and added glycerol stocks.
11:45-
We cultured and shook it at 37 degrees Celsius.
