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Team:Chiba/Notebook/Calendar/22 September 2009
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(21_September_2009 <|>23_September_2009)
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To judge character of LuxR mutants
Yesterday's operation is here.
Today's operation
We transplanted E.coli, which has been cultured on NC filter, to solid medium which contains each concentration of AHL.
AHL concentration is : 0, 10, and 1000 nM
We decided this time is T=0 and observed condition of fluorescence every 30 min.
以下に写真があります。
LuxR Mutantの個性決める実験の写真だけうｐ
22:35 Start
 (1) AHL 0 nM |
 (2) AHL 10 nM |
 (3 )AHL 1000nM |
23:05
 (1) AHL 0 nM |
 (2) AHL 10 nM |
 (3 )AHL 1000nM |
23:35
 (1) AHL 0 nM |
 (2) AHL 10 nM |
 (3 )AHL 1000nM |
24:05
 (1) AHL 0 nM |
 (2) AHL 10 nM |
 (3 )AHL 1000nM |
24:35
 (1) AHL 0 nM |
 (2) AHL 10 nM |
 (3 )AHL 1000nM |
25:05
 (1) AHL 0 nM |
 (2) AHL 10 nM |
 (3 )AHL 1000nM |
25:35
 (1) AHL 0 nM |
 (2) AHL 10 nM |
 (3 )AHL 1000nM |
26:05
 (1) AHL 0 nM |
 (2) AHL 10 nM |
 (3)AHL 1000nM |
26:35
 (1) AHL 0 nM |
 (2) AHL 10 nM |
 (3 )AHL 1000nM |
