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Team:Chiba/Notebook/Calendar/3 September 2009
From 2009.igem.org
(Difference between revisions)
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振とうしている間にT=0の時点での試料のOD値および蛍光強度を測定した。/While shaking, we measured OD and GFP fluorescence intensity of sample. | 振とうしている間にT=0の時点での試料のOD値および蛍光強度を測定した。/While shaking, we measured OD and GFP fluorescence intensity of sample. | ||
| - | |||
| - | :<table width=" | + | OD |
| - | :<td width=" | + | :<table width="500" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr> |
| - | :<td width=" | + | :<td width="100">0 μM AHL</td> |
| - | :<td width=" | + | :<td width="100">0.597</td> |
| - | :<td width=" | + | :<td width="100">0.590</td> |
| + | :<td width="100">0.589</td> | ||
| + | :<td width="100">0.043(control)</td> | ||
:</tr> | :</tr> | ||
:<tr> | :<tr> | ||
| - | :<td> | + | :<td>100 μM AHL</td> |
| - | :<td> | + | :<td>0.592</td> |
| - | :<td> | + | :<td>0.595</td> |
| + | :<td>0.591</td> | ||
:</tr> | :</tr> | ||
:</table> | :</table> | ||
| - | |||
| - | :<table width=" | + | GFP Fluorescence Intensity |
| - | :<td width=" | + | :<table width="500" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr> |
| - | :<td width=" | + | :<td width="100">0 μM AHL</td> |
| - | :<td width=" | + | :<td width="100">2.218</td> |
| - | :<td width=" | + | :<td width="100">2.043</td> |
| + | :<td width="100">2.099</td> | ||
| + | :<td width="100">1.748(control)</td> | ||
:</tr> | :</tr> | ||
:<tr> | :<tr> | ||
| - | :<td> | + | :<td width="100">100 μM AHL</td> |
| - | :<td> | + | :<td>2.139</td> |
| - | :<td> | + | :<td>2.114</td> |
| + | :<td>2.121</td> | ||
:</tr> | :</tr> | ||
:</table> | :</table> | ||
| - | |||
| - | :<table width=" | + | 18:30 プレートに試料をとり、OD値と蛍光強度を測定した。 |
| - | :<td width=" | + | |
| - | :<td width=" | + | OD |
| - | :<td width=" | + | :<table width="400" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr> |
| - | :<td width=" | + | :<td width="100">0.624</td> |
| + | :<td width="100">0.635</td> | ||
| + | :<td width="100">0.613</td> | ||
| + | :<td width="100">0.040</td> | ||
:</tr> | :</tr> | ||
:<tr> | :<tr> | ||
| - | :<td> | + | :<td>0.631</td> |
| - | :<td> | + | :<td>0.617</td> |
| - | :<td> | + | :<td>0.601</td> |
:</tr> | :</tr> | ||
:</table> | :</table> | ||
| - | |||
| - | :<table width=" | + | GFP Fluorescence Intensity |
| - | :<td width=" | + | :<table width="400" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr> |
| - | :<td width=" | + | :<td width="100">2.119</td> |
| - | :<td width=" | + | :<td width="100">2.035</td> |
| - | :<td width=" | + | :<td width="100">2.038</td> |
| + | :<td width="100">1.718</td> | ||
:</tr> | :</tr> | ||
:<tr> | :<tr> | ||
| - | :<td> | + | :<td>2.044</td> |
| - | :<td> | + | :<td>2.183</td> |
| - | :<td> | + | :<td>2.105</td> |
:</tr> | :</tr> | ||
:</table> | :</table> | ||
| + | |||
| + | |||
| + | |||
| + | 19:24 同様 | ||
| + | |||
| + | OD | ||
| + | :<table width="400" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr> | ||
| + | :<td width="100">0.741</td> | ||
| + | :<td width="100">0.724</td> | ||
| + | :<td width="100">0.717</td> | ||
| + | :<td width="100">0.040</td> | ||
| + | :</tr> | ||
| + | :<tr> | ||
| + | :<td>0.718</td> | ||
| + | :<td>0.698</td> | ||
| + | :<td>0.678</td> | ||
| + | :</tr> | ||
| + | :</table> | ||
| + | |||
| + | |||
| + | GFP Fluorescence Intensity | ||
| + | :<table width="400" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr> | ||
| + | :<td width="100">2.071</td> | ||
| + | :<td width="100">2.050</td> | ||
| + | :<td width="100">2.102</td> | ||
| + | :<td width="100">1.666</td> | ||
| + | :</tr> | ||
| + | :<tr> | ||
| + | :<td>2.449</td> | ||
| + | :<td>2.263</td> | ||
| + | :<td>2.344</td> | ||
| + | :</tr> | ||
| + | :</table> | ||
| + | |||
| + | |||
| + | |||
| + | 20:23 同様 | ||
| + | |||
| + | OD | ||
| + | :<table width="400" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr> | ||
| + | :<td width="100">0.774</td> | ||
| + | :<td width="100">0.742</td> | ||
| + | :<td width="100">0.742</td> | ||
| + | :<td width="100">0.040</td> | ||
| + | :</tr> | ||
| + | :<tr> | ||
| + | :<td>0.735</td> | ||
| + | :<td>0.723</td> | ||
| + | :<td>0.760</td> | ||
| + | :</tr> | ||
| + | :</table> | ||
| + | |||
| + | |||
| + | GFP Fluorescence Intensity | ||
| + | :<table width="400" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr> | ||
| + | :<td width="100">2.158</td> | ||
| + | :<td width="100">2.227</td> | ||
| + | :<td width="100">2.364</td> | ||
| + | :<td width="100">1.646</td> | ||
| + | :</tr> | ||
| + | :<tr> | ||
| + | :<td>2.815</td> | ||
| + | :<td>2.767</td> | ||
| + | :<td>2.756</td> | ||
| + | :</tr> | ||
| + | :</table> | ||
| + | |||
| + | |||
| + | |||
| + | 21:18 | ||
| + | |||
| + | OD | ||
| + | :<table width="400" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr> | ||
| + | :<td width="100">0.861</td> | ||
| + | :<td width="100">0.813</td> | ||
| + | :<td width="100">0.825</td> | ||
| + | :<td width="100">0.039</td> | ||
| + | :</tr> | ||
| + | :<tr> | ||
| + | :<td>0.755</td> | ||
| + | :<td>0.818</td> | ||
| + | :<td>0.849</td> | ||
| + | :</tr> | ||
| + | :</table> | ||
| + | |||
| + | |||
| + | GFP Fluorescence Intensity | ||
| + | :<table width="400" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr> | ||
| + | :<td width="100">2.062</td> | ||
| + | :<td width="100">2.775</td> | ||
| + | :<td width="100">2.673</td> | ||
| + | :<td width="100">1.814</td> | ||
| + | :</tr> | ||
| + | :<tr> | ||
| + | :<td>3.615</td> | ||
| + | :<td>3.510</td> | ||
| + | :<td>3.453</td> | ||
| + | :</tr> | ||
| + | :</table> | ||
| + | |||
| + | |||
| + | |||
| + | 22:56 中止 | ||
Revision as of 07:13, 14 September 2009
(2_September_2009 <|>4_September_2009)
- 本培養/main culture
10:30 昨日作成したAmp-Cm液体培地30 mLとプレ培養した溶液300 μLを三角フラスコにとり、37℃で振とうした。/we took the liquid medium that we had made yesterday to conical flask 30 mL and added prior culture 300 uL, then started shake culture at 37 degrees Celsius.
- グリスト作成/Making glycerol stock
50%グリセロール300 μLとプレ培養した溶液300 μLを1.5 mLマイクロチューブにとり、Deep Freezerに保存した。/We kept mixture of 300 μL of 50% glycerol and 300 μL of solution which is cultured yesterday in deep freezer.
- トランスファーカーブ作成実験/Making transfer curves
16:00 本培養が終わった溶液を10倍希釈し、48穴Deep wellに入れた。/We put the solution that has completed main culture in 48 deep wells.
17:15 48穴Deep wellを30℃で振とうした。/We shook 48 deep wells at 30 degrees Celsius.
振とうしている間にT=0の時点での試料のOD値および蛍光強度を測定した。/While shaking, we measured OD and GFP fluorescence intensity of sample.
OD
0 μM AHL 0.597 0.590 0.589 0.043(control) 100 μM AHL 0.592 0.595 0.591
GFP Fluorescence Intensity
0 μM AHL 2.218 2.043 2.099 1.748(control) 100 μM AHL 2.139 2.114 2.121
18:30 プレートに試料をとり、OD値と蛍光強度を測定した。
OD
0.624 0.635 0.613 0.040 0.631 0.617 0.601
GFP Fluorescence Intensity
2.119 2.035 2.038 1.718 2.044 2.183 2.105
19:24 同様
OD
0.741 0.724 0.717 0.040 0.718 0.698 0.678
GFP Fluorescence Intensity
2.071 2.050 2.102 1.666 2.449 2.263 2.344
20:23 同様
OD
0.774 0.742 0.742 0.040 0.735 0.723 0.760
GFP Fluorescence Intensity
2.158 2.227 2.364 1.646 2.815 2.767 2.756
21:18
OD
0.861 0.813 0.825 0.039 0.755 0.818 0.849
GFP Fluorescence Intensity
2.062 2.775 2.673 1.814 3.615 3.510 3.453
22:56 中止
※写真は本培養の開始までです。
